Olympus KHC User manual

MODEL KHC INSTRUCTIONS
Contents
I. Standard Set 1
IT. Specifications .. ······· .. · .. ·····.... ·· ....···· .. ·· .... ·· .. ··· .. · .. · .. ····· .. ·.... ·· .. ······ .. ·1
m. Name of Parts·················································································2
N. Optical Path .. ·· .... ·· .. ····.... ·· ·· ···· .. · .. · .. · .. · .. ·· .. · ·· ·· · .. ·· .. · · 2
V. Structure and Assembly .. ·· ·· .. ·· ·3
VI. Method of Operation .. · · .. ·· ·4
A. Smoothening of Coarse Adjustment knob
B. Light Source
C. Focusing
D. Interpupillary Distance and Diopter Adjustments
E. Use of Oil Immersion Objective
F. Use of Condenser
G. Change of Bulb
vll. Optical Characteristics 6
W. Major Troubles with Microscope, their Possible Causes
and Applicable Steps to be Taken ·..· .. ··7
IX. Important Points to Remember · .. ·8

I. Standard Set
Main Body: (built-in light source, stage and revolving nosepiece)
Binocular Head
Objectives: 4x, lOx, 40x, lOOx,
Eyepiece ; Bi WFlOx
Condenser
Filter
Spare Lamps ; 20W
Eyepiece tube cap
Dustproof Cap (for projection lens)
Cargille Oil
Vinyl cover
Certificate of Inspection
IT. Specifications
1 set
1 set
lea.
1pr.
1pc.
1pc.
3pcs.
2pce.
1pc.
1 btl.
1pc.
1 copy
Height
Width
Depth
Net weight
Head: Binocular Head
Revolving nosepiece :
Focusing:
Coarse abjustmemt :
Fine adjustment:
Condenser:
Illumination:
Stage:
Dimensions :
45
0inclination,
rotatable through 360
0
horizontally;
Stopper to fix the head at any designed position.
Range of interpupillary distance adjustment: 55.5 - 74mm.
Dioptric adjustment is possible.
Quadruple indicator for Objectives' position
Vertical movement stage
Range of operation 28mm
Range of operation 2mm
N. A. 1.25
Variable iris-diaphragm
Range of vertical movement 15mm
Pre-centered 20W light source is built inside the base.
Coaxial single-handle square mechanical stage
Range of operation: Back-forth 30mm
Right-left 70mm
355mm
165mm
205mm
approx. 5.5kg without case
-1-

ill. Name of Parts
Eyepiece
Binocular
head
Coaxial knob for
slide monipulation
Coaxial coarse and
fine adjustment knobs
N. Optical Path
Objective
Stage
Condenser
Projection lens
Filler
Prism
Projection
Lens
Lamp
- 2 -

V. Structure and Assembly
A. Main Body
Head mount
Head i~ atta('hl~d upon this mount.
Arm
Slage back-forth
movement knob
Spl'('iml'n ca" be mO\'t"d back
and forth in "rangll of 30mm.
Slage right-left
movement knob
Sp('cimen can be mO\'cd ri..::hl
and l .. h in ,) ranlo1l' uf 70mm.
Pre-set focusing lock
Fine adjustment knob
StalCe can be moved \'erticaJly
in minute mo\'l'mcnt in il range
of 2mm.
Power source cord
1. Revolving Nosepiece
Revolving nosepiece
Stage clip
Stage
Condenser mount
Condenser locking screw
Condenser vertical movement knob
Conden~er can hl' mon .. J uv
and down in arange of lSmm
Projection lens
This is adetachable screw-in
type lens. This can be
remo\'cd when mirror is used.
Illumination switch
Push one end of the switch
marked with awhite dOl.
to switch on the illuminator.
The quadruple nosepiece moves very lightly and click-stops in the right position for the
optical path.
Mount the objectives in such an order that, when the nosepiece is turned clockwise
during observation, an objective of the next higher magnification will come into position.
2. Pre-set focus lock
When the lock is tightened, it will hinder the movement
of the stage further upward from that point. When the
stage is moved down once and again brought up closer
to the lens, it will stop at the position set by the lock.
This will help prevent the speciment to crash against
the objective and further facilitates an easy focusing
for the observer.
3 -

3. Condenser
Insert the condenser (1) from underneath into the con-
denser
(g) and secure it in position by tightening the
locking screw (3J.
B/';-':Binocular Head
CD
Dioptric adjustment ring
Turn this ring to adjust
the dioptric difference
between the right and
left eyes.
Eyepiece tube seal
Holdin~ these seats,
pull the lubes apart or
push them closer (or
interpupilbry distance
adjustmpnt.
Head mounting ring
Tht' head is £ixt.'d 10 th ..
main booy by thi~ ring.
Interpupillary dislance scale
/Eyepiece
tube
Put in the eyepiece.
Clamping screw
Th~ head is fixed· at tll<'
desired position by this screw.
oHow to Mount the Head
Place
the head on the receptacle at the upper end
of the arm and turn the mounting ring te fix it.
VI. Method of Operation
A. Smoothening of Coarse Abjustment Knob
This coarse adjustment knob operates rather heavily
However. such can be adjusted to the operator's
preference. Hold both knobs firmly, turn them
counterclo kwise (as shown in the illustration) to make
it operate lightly, and clockwise heavily.
B. Light Source
Connect the cord (from the base) with the power source. Press the switch on the white-
dotted side to turn on the lamp.
- 4 -

c. Focusing
1. Turn the fine adjustment knob to bring the index line to the middle of the two lines
(movement range).
2. Place the specimen on tne stage and secure it with the clips.
3. Put the lOX objective in position.
4. Looking from side of the microscope, move the stage upward carefully by the coarse
adjustment knob until the specimen is brought very close to the tip of objective.
(** Be most careful not to hit the objective.)
5. Looking through the eyepiece, gradually lower the stage to obtain rough focus.
6. Turn the nosepiece to set the desired objective in position. Accrately focus with the
fine adjustment knob.
7. The specimen may be moved by the slide manipulation knob, built on the stage so
that the particular spot to be observed comes into the center of the view field.
**** For convenience in the later operations, tighten up the pre-set focus lock when the
specimen is roughly in focus at step 5, above. (See for V -A-2, Pre-set focus lock)
D. Inter-pupillary Distance and Dioptor Adjustments
1. The interpupillary distance is adjusted by
a. Holding both eyepiece tube seats,
b.
Looking into the eyepieces, and
c.
Pulling apart or pushing closer the seats until the view field is observed in one.
When
the adjustment is made, read the figure on the scale; this will make the
adjustment easier from the next time on, as it can be done by merely setting the
tUbes
at the given figure.
2. Diopter is adjusted by
a. Looking through the right eyepiece with the right eye,
b. Focusing on
the specimen by the coarse and fine adjustment knobs,
c. Then
looking into the left eyepiece with the left eye, and
d.
Turning the diopter ring until the specimen is brought into focus correctly.
When looking into the left eyepiece with the left eye, do not touch the coarse or fine
adjustment knob.
E. Use of Oil Immersion Objective
First, bring the specimen into focus with a low magnification objective (lOX). Apply a
drop of cargille oil on the specimen as well as at the tip of the oil immersion objective.
Turn the nosepiece to bring the oil immersion objective above the specimen. This can be
done
without the fear of crashing them into each other. Focus accurately with the fine
adjustment knob and make observations. Upon completion of observations, carefully wipe
off cargille oil on the oil immersion objective with apiece of gauze moistened with xylol.
**** Cargille oil left on the objective can ruin the lens greatly. An utmost care is
required in this point.
-5-

F. Use of Condenser
If an objective of low magnification (less than 10X) is in use, lower the condenser
slightly to avoid an uneven illumination as well as for a better result. The upper-lens
of the condenser may be removed. Removal of the upper-lens gives a better result than
lowering of the condenser. When the oil-immersion objective is in use, move it up to
the top of its movement range.
G. Change of Bulb
Lay down the microscope, and loosen two screws CD underside the base with a coin
or something like that. Remove the cover and change the used lamp with anew one.
vI. Optical Characteristics
Objective
Magnification
I
4x
1
10x
1
40x
I
100x
"",
".
Numerical
I
0.1
1
0.25
I
0.65
1
1.3
'"
aperture (N. A.)
'"
Working distance
I
19.771
II
",5.1
0.4 0.11
"'"
(W. D.)
Eyepiece
"'.
Focal length
I
29.2
i
15.7
I
4.3
I
1.8
""
Total magnification
I
40x
I
100x
I
400x
11'OOOX
WF 10x (Number
Depth of focus
I
112.5
I
18.0
I
2.0
I
0.6
of viewfield 18)
I
Actual viewfield
I
4.5
I
1.8
I
0.45
I
0.18
- 6 -

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'I" ~ "0
• OLYMPUS OB-JECTIVE HAS
ITS COLOUR BAND
THE OLYMPUS OBJECTIVE, you just
purchased, has a colour band on it.
This new step has been adopted for
your convenience, that you may unders-
tand the magnification, if you only
see the colour, without looking at
the magni ficat ion number engraved on
the tube. Though there is no ment ion
about the colour band in the instruction
booklet, upon your recogni tion of our
new colour system, the olympus products
wait for your further efficient usage.
1= !/&J JfI
/'I'i: ~
If]
l-a'
,t;
~
lffi
~
Biological
use
Metallurgical use
Range
of
Colour
Plan
Fl Ach Plan
Ach magni
ficat ion
~
Purple 1.3X 1.3X 0
-Less than 2X
~
Brown
2X
2X 2X -Less
than 4X
,n;
Red
4X 4X 5X
5X
4 X -Less
than
7X
6X
.m
Orange IOX IOX IOX IOX
7X -Less
than 20X
1l.
Yellow
20X 20X 20X 20X 20X -Less
than 40X
'1t~
Brilliant
green 40X 40X 40X 40X 40X 40X -Less
than 60X
,~.f,1c
Cobalt blue 60X 60X
- Less thanlOOX
ik~j'
Light blue lOO X lOO X lOO X lOO X lOO XlOOX. and
over
is* Colour band
OLYMPUS OPTICAL CO .. LTD.

VIII. Major troubles with microscope, their possible
causes and applicable steps to be taken.
Troubles
IPossible causes 1Steps
to be taken
Slipping down of the
Loosened coarse See
Smoothening of Coarse
stage by itself
adjustment knob Adjustment knob
and
pro-
perly adjust it.
Coarse Adjustment
1. Axis
of Coarse
Adjust- 1. Take
reverse steps of
knob too heavy
ment knob overly
the above.
tightened
2. Hardened grease on the 2.
Notify the
sliding surface.
manufacturer.
-
Fine Adjustment knob
Hardened grease on the
Notify the manufacturer.
too heavy
sliding surface
Dust/Oirt on objective
1.
Eyepiece tubes are left
Wipe off the top lens of the
without eyepieces or
objective with clean gauze;
cap.
do not
touch inner lenses
2.
Fingerprints left on the
but notify the manufacturer
lens;
Lens
hitting the speci-
men;
or cargille oil left on
the
oil-Immersion lens.
Dust/Dirt on eyepiece 1. Careless storage.
1.
Be sure to store the
eyepieces after use in
the provided container
or in a desiccator
2. Fingerprints left on the
2.
Wipe off with clean
lens
surfece
gauze. Do not try to
disassemble the
eyepiece. Any change in
arrangement of lenses
can
deteriorate the
entire opical efficiency.
Scratches on the lens Lens was wiped with apiece
Immediately notify the
of cloth or leather while
manufacturer. The lens will
dust or filth is still left on. be replaced
at the actual
cost.
- 7 -

IX. Important points to remember
Dampness and dust is a taboo for the microscope. But, frequently, alab where the
microscope is used is not free of such. It is the best to store microscope after each use in
the provided container. If frequent observations make it impossible, at least cover the
instrument with the provided vinyl cover.
The
objectives and eyepieces should best be stored in a desiccator. Also recommended
is
to place apack of silicagel in the container. After the eyepiece is removed from the
instrument, be sure to cover the eyepiece tube with the provided cap. Never attempt to
disassemble or repair the mechanical parts of the microscope. It must be done by
specialists. Cleaning must be performed with utmost care. For expample, dust off with a
soft brush or blow off by a rubber ball where hands cannot reach.
Mirror (optional accessory)
In general, microscopy with KHC is conducted with the projection lens on. However, for
special illumination effect this mirror can be used for high magnification lens. and the other
side, concave surface, is good for observation with low magnification objl'ctives.
- 8 -