Nikon SMZ1000 User manual

Stereoscopic Zoom Microscope
SMZ1000 / SMZ800
Instructions
M248 E 06.2.NF.3

1
Thank you for purchasing the Nikon product.
This instruction manual is written for the users of Nikon
Stereoscopic Zoom Microscopes.
To ensure correct usage, read this manual carefully before
operating the instrument.
•It is prohibited to reproduce or transmit this manual in part or
whole without Nikon’s expressed permission.
•The contents of this manual are subject to change without notice.
•Although every effort has been made to ensure the accuracy of
this manual, if you note any points that are unclear or incorrect,
contact your nearest Nikon representative.
•Some of the products described in this manual may not be
included in the set you have purchased.
•If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be
impaired.
Warning/Caution Symbols Used in This Manual
Though Nikon products are designed to provide you with the
utmost safety during use, incorrect usage or disregard of the
instructions may cause personal injury or property damage. For
your own safety, read the instruction manual carefully and
thoroughly before using the product. Do not discard this manual.
Always keep it near the product for easy reference.
Inside this instruction manual, safety instructions are indicated
with the symbols shown below. Be sure to follow the instructions
marked with these symbols for your safety.
Symbol Meaning
Disregarding instructions marked with this
symbol may lead to death or serious injury.
Disregarding instructions marked with this
symbol may lead to injury or property
damage.
WARNING
CAUTION

2
WARNING
1. Intended use of this product
This product is intended only for microscopy. Do not
use it for any other purpose.
2. Do not disassemble
Disassembly may result in damage to the instrument.
Never disassemble any part except as described in this
operation manual. Contact your Nikon representative if
you notice any malfunction of this instrument.
3. Check the input voltage
When using an illuminator, check that the input voltage
displayed on the power supply of the illuminator
matches the operating voltage. Contact your Nikon
representative if the displayed voltage does not match the
operating voltage. Use of an improperly matched
illuminator may result in damage to equipment.
4. Power cord
Always turn the power switch off and unplug the power
cord when replacing the lamp of an illuminator as failure
to do so may result in electric shock or equipment
damage.
5. Heat from the light source
When using an illuminator, do not place cloth or paper or
highly flammable materials, such as gasoline, benzene,
thinner or alcohol, near the lamp as there is a danger of
fire.
•Always use lamps that are of the specified rating only.
For the lamp rating, please refer to the operation
manual.
•Always turn the power switch off and unplug the
power cord when replacing the lamp of a illuminator
as failure to do so may result in electric shock or
equipment damage.
Be sure to always follow these guidelines.

3
CAUTION
1. Caution when replacing lamps
The lamp and the surrounding area become very hot
during and immediately after using the illuminator. Be
careful not to burn yourself. In addition, when replacing
a lamp be sure the lamp has cooled sufficiently.
2. Check the light source
Always use lamps that are of the specified rating only.
Use of a lamp having a different specification may result
in damage to equipment. For the lamp rating, please
refer to the operation manual.

4
1. Installation location
Note the following points when installing the stereoscopic
microscope.
•Install the microscope in a location with the temperature between
0° and 40°C, and humidity of less than 80%. If installed in a hot
and humid location, mold may form on the lenses or condensation
may occur inside, resulting in reduced performance or damage to
the microscope.
•Do not install the microscope in a location subject to direct
sunlight.
•Install the microscope in a location that is not subject to vibration.
•Install the microscope in a location that is free from dust and dirt.
2. Handle the microscope carefully
The stereoscopic microscope is a precision optical instrument.
Handle it carefully and do not subject it to impact. Shock from
impact during transportation or operation as well as forcible
operations may cause damage to the instrument.
3. Cleaning the lenses
Do not let dust, fingerprints, etc. to get on the lenses. Dirt on the
lenses will adversely affect the view of the image. If any lenses
become dirty, clean them as described below.
•Use an air blower to blow dust away. If this does not suffice, brush
away the dust with a soft brush or gently wipe it away with a piece
of gauze.
•Only if the lenses become dirty with fingerprints or grease stains,
slightly dampen a piece of soft, clean cotton cloth with absolute
alcohol (ethyl alcohol or methyl alcohol) and gently wipe away the
dirt. Do not use the same part of the cloth more than once.
•Since absolute alcohol is highly flammable, be careful in handling
it so that it does not ignite.
•Observe the manufacturer’s handling instructions when handling
absolute alcohol.

5
Be sure to always follow these guidelines.
4. Cleaning painted or plastic parts
Use of silicon cloth is recommended when cleaning painted parts,
plastic parts and printed parts. If such a part becomes excessively
dirty, wipe it gently with gauze dampened in a mild detergent
solution. Do not use organic solvents (such as alcohol, ether, or paint
thinner) as this may result in deformation of the part or remove of
lettering.
5. Storage
Store the microscope in a location with low humidity where mold is
unlikely to form. Do not store the microscope in a location subject
to direct sunlight or high temperature and humidity. During storage,
place a plastic cover over the equipment to prevent dust
accumulation.
6. Regular inspections
Regular inspections are recommended in order to maintain peak
performance. Please consult your Nikon representative for details
about regular inspections.

6
Contents
Warning/Caution Symbols Used in This Manual ...........................1
Be sure to always follow these guidelines. ....................................2
WARNING ..........................................................................................2
CAUTION ...........................................................................................3
INomenclature ...................................................................................8
II Assembly .........................................................................................12
III Usage................................................................................................ 15
1Preparations For Observation ......................................................15
1. Adjust the torque of the focus knob.......................................15
2. Adjust the interpupillary distance. .........................................15
3. Adjust the diopter. .................................................................16
2Focusing ......................................................................................16
1. Check the working distance...................................................16
2. Focus on the sample. .............................................................16
3Zoom ...........................................................................................17
1. Change the zooming magnification. ......................................17
4If You Cannot Focus On The Sample Though The Zooming
Body Is At The Highest Position. ................................................18
IV Using Accessories ........................................................................19
1Reticles ........................................................................................19
2Eyepiece Tube ............................................................................. 19
1. P-BTL low eye-level eyepiece tube ....................................... 19
2. P-BERG tilted eyepiece tube ................................................. 20
3P-IER Eyelevel Riser .................................................................. 20
4Plan 1X ERGO Objective ...........................................................21
5SM-S4L 4 X 4 Stage L ................................................................ 22
VIlluminator .......................................................................................23
1Halogen Illuminators ................................................................... 23
1. Lamp ......................................................................................24
2. Attaching G-LS to the stand [C-PS & C-PSC] ...................... 25
3. Arm for halogen illuminators ................................................25
4. Power supplies ....................................................................... 27

7
5. Lighting area adjustment [G-LS]...........................................29
6. Attaching filters [C-DSLS] ....................................................29
2P-ICI2 Coaxial Episcopic Illuminator .........................................29
VI Photomicrographic and TV-related Accessories ..................32
1. Photomicrographic magnification ......................................... 33
2. Attaching the beam splitter ....................................................33
3. Photo reticles ......................................................................... 34
4. Photomicrography procedure ................................................ 34
Table 1: Total Magnification and Real Field ...................................... 36
Table 2: Observable Sample Heights [mm] .......................................38

8
INomenclature
The figures on p.8 & 9 show a SMZ1000 zooming body, with P-BT
binocular eyepiece tube, C-W 10X eyepieces, Plan Apo 1X objective,
C-FMC focusing mount, and C-PS160 plain stand.
Eyepieces
10X, 15X, 20X and
30X eyepieces are
available.
Diopter ring
Refer to “Adjust the
diopter” on p.16.
Eyepiece tube
clamp screw
Zooming body
Outside diameter of the
part to be mounted the
focusing mount: ø76
Groove for mounting
accessories
Holds the Fluorescent
ring illuminator, Fiber
optic ring illuminator
and the like. Outside
diameter: ø60
Plan 1X, Achro 0.5X,
Plan 1X ERGO, Plan
Apo 0.5X, Plan Apo 1X,
ED Plan 1.5X and ED
Plan 2X objectives are
available. HR Plan Apo
series objectives for
SMZ1500 can be used
also, but the magnifi-
cation in this case
becomes 1.25 times the
magnification indicated
on the objective itself.
Refer to Table 1 on p.36.
Stage plate clamp
screw
Fixes the stage plate
to the stand base.
Zooming body
clamp screw
Fixes the zooming body
to the focusing mount.
Objective
Zooming knob
Changes the
magnification of the
sample image. Refer
to “Zoom” on p.17.
P-BT Binocular
eyepiece tube
Eyepiece sleeve
P-BTL low eye-level
eyepiece tube and P-
BERG tilted eyepiece
tube are also
available.
Coarse focus knob
Fine focus knob
C-PS160 Stand base
Various stands are
available.
Refer to “Focusing”
on p.16.
Refer to “Focusing”
on p.16.
Stage plate
The black or the
white side can be
chosen according to
the sample.
Screw for mounting
accessories
Holds the 1/4 lambda
plate and the like.
The screw on the
inside diameter:
M55 ×0.75
Stand

9
I
Screw hole for mounting
an Epi-arm for halogen
illuminator
Located on both sides
of the focusing mount.
Holds the G-EIA
flexible arm or Epi-arm.
Located on both sides
of the stand base. M5
C-FMC Focusing mount
Screw hole for
mounting accessories
Clamp tool
Used for the stage plate clamp
screw, eyepiece tube clamp
screw, etc. Same size as the
small hexagonal wrench
supplied with the zooming body.
Stored in the top of the pillar.
Zooming knob clicks ON/OFF
screw (inside)
Refer to “ON/OFF of the Zooming
Knob Clicks” on p.17.
Torque adjustment ring
(Innermost ring)
Pillar
Adjusts the torque of the
focus knob. (Refer to
“Adjust the focus knob
torque” on p.15.)
Pillar clamp screw
(Inside)
Focusing mount
clamp screw
Fixes the focusing mount
to the pillar.

10
Eyepiece
10X, 15X, 20X and
30X eyepieces are
available.
Diopter ring
Refer to “Adjust the
diopter” on p.16.
Eyepiece tube
clamp screw
Zooming body
Outside diameter of the
part to be mounted on
the arm: ø76
Groove for mounting
accessories
Holds the Fluorescent
ring illuminator, Fiber
optic ring illuminator and
the like.
Outside diameter: ø60
Screw for mounting
accessories
Holds the 1/4 lambda
plate and the like.
The screw on the inside
diameter: M55
× 0.75
Zooming body
clamp screw
Fixes the zooming
body to the arm.
Objective
Plan 1X, Achro 0.5X, Plan
1X ERGO, Plan Apo 0.5X,
Plan Apo 1X, ED Plan
1.5X and ED Plan 2X
objectives are available.
HR Plan Apo series
objectives for SMZ1500
can be used also, but the
magnification in this case
becomes 1.25 times the
magnification indicated on
the objective itself. Refer
to Table 1 on p.36.
Zooming knob
Changes the
magnification of the
sample image.
Refer to “Zoom” on
p.17.
P-BT Binocular
eyepiece tube
Eyepiece sleeve
P-BTL low eye-level
eyepiece tube and P-
BERG tilted
eyepiece tube are
also available.
Focus knob
Refer to “Focusing”
on p.16.
Arm fixing screw
Holds the arm. The
position of the arm
can be chosen from
either high or low.
Hole for clip
Holds the clip for
retaining the sample.
C-PS Focusing
stand
Various stands
available.
Stage plate
The black or the
white side can be
chosen according to
the sample.
Vertical slider
Arm
The illustrations on p.10 & 11 show a SMZ800 zooming body, with P-BT
binocular eyepiece tube, C-W 10X eyepieces, Plan 1X objective, and
C-PS plain focusing stand.

11
Screw hole for mounting an
Epi-arm for halogen illuminator.
Screw holes are located on
both sides of the arm.
Holds the G-EIA flexible
arm or Epi-arm.
Screw hole for mounting
6V-10W halogen illuminator
Holds the G-LS 6V-10W
halogen illuminator.
Hexagonal wrench
hanger
Holds the supplied
hexagonal wrenches.
Zooming knob clicks ON/OFF screw
(inside)
Refer to “ON/OFF of the Zooming
Knob Clicks” on p.17.
Hexagonal wrench
(Large)
Pillar
Supplied with the
focusing stand.
Used for changing
the height of the arm.
Hexagonal wrench
(Small)
Supplied with the
zooming body. Used
for the eyepiece tube
clamp screw and
zooming knob clicks
ON/OFF screw.
I
Nomenclature

12
II Assembly
☞• An equipment model name in the bold-faced brackets [ ] indicates
that the following description is exclusively for that model.
1[C-PS160]
Attach the pillar to the stand.
Insert the pillar straight into the stand with the
groove of the pillar located down as far as the
pillar reaches to the bottom. Clamp the pillar
using the clamp tool.
2Place the stand on the level surface.
3Mount the stage plate.
[C-PS160]
Fit the stage plate into the stand base. Tighten
the stage plate camp screw using the clamp
tool to fix the stage plate.
[C-PS & C-PSC]
Fit the stage plate into the stand base while
pushing it against the rim in the direction
shown by the arrow in the illustration.
4[C-PS160]
Fix the C-FMC focusing mount to the
pillar.
Face the focusing mount to the front and
tighten the focusing mount clamp screw.
Note) When fixing the focusing mount to the
upper part of the pillar, securely
tighten the clamp screw so as not to
fall the focusing mount.
Pillar
Groove

13
II
5[C-PS & C-PSC]
The stand arm can be lowered. (If you
do not need to lower the arm, jump to
step 6.)
Use the hexagonal wrench (large - supplied
with the focusing stand) to loosen the arm
fixing screw. Reattach the arm using the
screw hole at the lower side of the vertical
slider. The arm will become 55 mm lower
than its original position. Before tightening
the screw, make sure that the two pins on the
arm fit in the grooves on the vertical slider.
6Mount the zooming body.
Lightly tighten the zooming body clamp
screw to hold the zooming body on the
focusing mount or arm.
Note) Do not over-tighten the zooming body
clamp screw since it may cause
damage to the instrument.
7Screw the objective into the bottom of
the zooming body.
8Mount the eyepiece tube.
Align the groove on the eyepiece tube with
the positioning pin on the zooming body, push
the eyepiece tube toward the back to attach.
Tighten it to the zooming body using the
supplied hexagonal wrench (small) or the
clamp tool supplied with the C-PS160.
Groove
Positioning pin
Groove
Pin

14
9Insert the eyepieces into the eyepiece sleeves.
Be sure that it is inserted all the way until it touches the end of the sleeve.
Note) When inserting the eyepiece, hold the rubber cover of the eyepiece.
Holding the diopter ring causes a failure. Especially, be careful
with the 10X eyepieces because their rubber covers obstruct the
view of the sleeve end.
10 The hexagonal wrenches and clamp tool can be stored in the
pillar of the stand.
Refer to the figures on p.9 and p.11.
Assembly II

15
III Usage
1Preparations For Observation
1Adjust the torque of the focus knob.
Adjust the torque of the focus knob so as not to fall down the zooming
body on its own weight.
[C-FMC]
Turn the torque adjustment ring using the
clamp tool. Turning the ring in the direction
of the arrow indicated on the side surface of
the focusing mount will increase the torque.
Note) • Do not twist the left and right focus
knobs.
• Do not turn the knob beyond its
turning limit.
• Do not turn the fine focus knob while
holding the coarse focus knob or vice
versa. Otherwise, the damage to the
instrument will result.
[C-PS and C-PSC]
Rotating the left and right focus knobs in the
opposite direction as shown in the illustration
will increase the torque.
2Adjust the interpupillary distance.
This adjustment should be performed every
time the observer is changed since the
interpupillary distance differs between
individuals. Adjust the interpupillary distance
so that the view field for each eye is merged
into one. Move while holding each sleeve
with both hands.
Increasing the torque.
(To reduce the torque,
turn the knob in the direction
opposite to the arrow.)
TORQUE
Increasing the torque.

16
3Adjust the diopter.
This adjustment should be performed every time the observer is changed
since the eyesight differs between individuals.
1Turn the diopter rings on both eyepieces to set them at the 0 position
(match the 0 line with the index line).
2Turn the zooming knob to the highest magnification. Focus on the
sample using the focus knob. (Refer to “Focusing”.)
3Turn the zooming knob to the lowest magnification. Peering
through the left eyepiece with the left eye, focus on the sample using
the diopter ring on the left eyepiece. Then, peer through the right
eyepiece with your right eye and focus on the sample using the
diopter ring on the right eyepiece.
4Repeat steps 2and 3until the image is kept focused even though the
zooming magnification is changed. This adjustment ensures sharp
image throughout the zooming range.
2Focusing
1Check the working distance.
The distance between the focus plane and the bottom of the objective is
called the working distance. Focusing is easier if beforehand the distance
between the specimen and the bottom of the objective is set to the working
distance. The working distance differs depending on the objective being
used. Please refer to Table 1 on p.36.
2Focus on the sample.
Turning the focus knob moves the zooming body up and down and brings
focus on the sample.
[C-FMC]
Using the fine focus knob will permit the delicate focusing. Especially, it
is effective for the high magnification observation.

17
III
Usage
3Zoom
1Change the zooming magnification.
Turning the zooming knobs on the left and right side of the zooming body
will change the magnification of the sample image.
■Total Magnification
The zooming knob has the indication of the zooming magnification.
The total magnification can be found by multiplying the magnification
of the objective times that of the eyepieces.
Note) When the HR Plan Apo series objective for the SMZ1500 is used,
the total magnification will be obtained by multiplying by 1.25.
■ON/OFF of the Zooming Knob Clicks
The clicks on the zooming knob can be
eliminated.
1Insert the supplied hexagonal wrench
(small) or the clamp tool supplied
with the C-PS160 into the screw hole
on the rear of the zooming body.
2Turning the inside screw in the
direction opposite to the arrow
eliminates the clicks (OFF). Turning
the same screw in the direction of the
arrow puts on back the clicks (ON).

18
Auxiliary
adapter
III
Usage
4If You Cannot Focus On The Sample Though
The Zooming Body Is At The Highest Position.
When you use the 0.5X objective or observe a tall sample, you may not be
able to focus on the sample though turning the focus knob to raise the
zooming body to its highest position.
[C-PS160]
Exchange the pillar for the C-EP extension pillar available on order.
Attach the C-EP extension pillar to the stand in the same manner as for the
standard pillar. (Hold the side having the groove downward. Refer to
item 1. on p.12.) Put the stopper ring on the pillar and then the focusing
mount. Tighten the focusing mount clamp screw.
Note) Contact the bottom surface of the focusing mount with the stopper
ring and securely tighten each clamp screw.
[C-PS & C-PSC]
Use the C-ER auxiliary adapter available as
an option. (This adapter can’t be used for the
SMZ1000.)
☞• Refer to the Table 2 on p.38 for the sample heights which may be
viewed.
Extension pillar
Correct Wrong
Stopper ring