Logos Celena S User manual

ONSTAGE INCUBATION SYSTEM
INSTALLATION GUIDE
This Installation Guide provides instructions for installing the onstage incubation system for the CELENA®S.
For detailed instructions, refer to the provided user manuals.
1. Connect all tubes and cables.
2. Fill the humidifying column with 130 mL ultrapure water.
3. Turn on all gases to 1 bar of pressure.
4. Turn on the gas mixer and temperature controller and set the settings as desired.
ORDER OF INSTALLATION
CONNECTIONS
Gas mixer
Inputs
-CO2: green tube CO2 tank
- Air/O2: blue tube air pressure generator
- N2: yellow tube air pressure generator
Outputs
- Blue tubes Humidifying Column
- Heating connection Humidifying column
- Humidity sensor Heated lid
Temperature controller
- Channel 1 Heated lid
- Channel 2 Heated plate
Humidifying
column
CO2 tank
Gas mixer Heated lid
Heated
plate
CH 2
HEATER
SENSOR
Air pressure
generator
N2 tank
(Optional)
Temperature
controller
VC1707-01
© 2017 Logos Biosystems, Inc. All Rights Reserved. Logos Biosystems | www.logosbio.com
1. Place the CELENA S vessel holder frame on a flat surface.
2. Place the heated plate in the vessel holder frame.
3. Place a heating insert in the heated plate.
4. Place the heated lid on top of the heated plate.
5. Place the assembled incubator on to the CELENA S stage and connect the vessel holder frame to the vessel holder frame arm.
STAGE ASSEMBLY

ONSTAGE INCUBATION SYSTEM
INSTALLATION GUIDE
This Installation Guide provides instructions for installing the onstage incubation system for the CELENA®S.
For detailed instructions, refer to the provided user manuals.
VC1706-01
© 2017 Logos Biosystems, Inc. All Rights Reserved. Logos Biosystems | www.logosbio.com
GAS MIXER TEMPERATURE CONTROLLER HUMIDIFYING COLUMN
AIR PRESSURE GENERATOR HEATED LID AND PLATE
Use a wash bottle to fill the humidifying
column. Don’t exceed 130 mL. Don’t let
the water fall below 50 mL during use.
Don’t leave any input ports open in the
gas mixer. Close unnecessary ports with
dead-end tubing.
Turn on the gas mixer only after the
input pressure of all gases have been
set to 1 bar.
MAX - 130 mL
MIN - 50 mL
After placing your sample onto the
CELENA S, wait 20 minutes before
starting a time-lapse experiment.
Only use the provided tubes, cables,
and plugs to ensure electrical safety.
IMPORTANT POINTS
MAIN COMPONENTS
HEATING INSERTS