Luigs & Neumann LNscope User manual

Operating Manual
LNscope
Upright microscope
www.luigs-neumann.com

1. Read this first
1.1 Before operation
Read the documentation carefully, especially with regard to all tips and safety markings. Do
not operate the system in a potentially explosive or humid environment. In the case of a visible
damage, do not switch on the system and contact Luigs & Neumann GmbH immediately.
1.2 Note
Reproduction of any part of this document in any form or by any means without prior written
authorization is prohibited.
1.3 Warranty
Luigs & Neumann warrants LNscope hardware, electronic and software against defects in
material and workmanship for a period of two years from the date of shipment.
All repairs should be carried out by Luigs & Neumann.
Replacements and repairs must be done using the freight prepaid by Luigs & Neumann.
Returned parts must be packed in their original boxes or in new boxes with similar standards
with regard to safe transport.
1.4 Warranty does not cover
- Any loss, damage and product malfunction caused by
misuse and improper or inadequate care.
- Consumables and maintenance items
1.5 Specifications
The LNscope is for indoor use only.
- Operating temperature................................... : 16°C - 30°C
- Relative humidity during operation................. : non-condensing
- Power requirements....................................... : 100/120/220/240 VAC, 50/60Hz
This system may be operated only on AC grounded power.
1.6 Facility Requirements
Follow these guidelines, setting up the LNscope system in order to get the best performance.
- The system be screwed to a stable, rigid surfacemust .
1.7 Harmonization legislation
All parts of the system comply with the essential requirements laid down in the harmonization
legislation mentioned below
Niederspannungsrichtlinie:
2014/35/EU Richtlinie des Europäischen Parlaments und des Rates vom 26. Februar 2014;
Amtsblatt der EU L96, 29/03/2014, S. 357 – 374 (ab 20.04.2016)
Low Voltage Directive:
2014/35/EU Directive of the European Parliament and of the Council of 26. February 2014
Official Journal of the EU L96, 29/03/2014, p. 357 – 374 (from 20.04.2016)
EMV-Richtlinie:
2014/30/EU Richtlinie des Europäischen Parlaments und des Rates vom 26. Februar 2014;
Amtsblatt der EU L96, 29/03/2014, S. 79 – 106 (ab 20.04.2015)
EMC Directive:
2014/30/EU Directive of the European Parliament and of the Council of 26. February 2014
Official Journal of the EU L96, 29/03/2014, p. 79 – 106 (from 20.04.2016)
1

The LNscope system has been developed for scientific research. This instrument has been
designed to be used to observe magnified images of specimens in routine and research
applications. Do not use this instrument for any purpose other than its intended use.
The device may not be rebuilt or dismantled for other purposes. Only trained persons should
work with this equipment. Failure to comply with the safety instructions and work procedures
described in the manual may result in personal injury or damage to the unit . These damages
are not covered by the guarantee. Due to on-going development, some illustrations in the
manual may differ from the supplied system. We welcome any user feedback that may result
in future improvement of this manual.
2.1 Cleaning System
1. When cleaning, the system must be disconnected from the power supply.
2.To clean the lenses and other glass components, simply blow dirt away using a
commercially available blower and wipe gently using a piece of cleaning paper
(or clean gauze). If a lens is stained with fingerprints or oil smudges, wipe it with
gauze slightly moistened with commercially available pure alcohol.
Since the pure alcohol is highly flammable,
it must be handled carefully.
Be sure to keep it away from open flames or
potential sources of electrical sparks –– for example,
electrical equipment that is being switched on or off.
Additionally remember to use it only in a well-ventilated room.
3. Never attempt to disassemble any part of the microscope.
4. When disposing of this unit, check the regulations and rules of your local
government and be sure to observe them.
2.2 Distribution configurations
The system is offered in two versions:
a - LNscope without Transmitted light unit (System for InVivo Setups)
b - LNscope with Transmitted light unit (System for Invitro Setups)
Accessories such as Olympus parts can be upgraded with any version.
2.3 Maintenance / Repair
The LNscope system is maintenance-free.
2.4 Warning Indication
A warning sticker is attached to a part where special precaution is required when handling and
using the system. Always heed the warning.
2. User instructions
2
Warning indication
position
Lamp housing (U-LH100-3/U-LH100IR)
(Warning against high temperature)

3. Table of Contents
1. Read this first............................................................................................
2. User instruction.........................................................................................
3. Table of Contents..........................……………………...............................
4. Instructions................................................................................................
5. Overview LNscope....................................................................................
6. LNscope-main body + accesories.............................................................
7. Mount the LNscope onto the Breadboard.................................................
8. Mount the transmitted light unit…............................ .................................
9. Mounting the condenser-carrier................................................................
10. Holder for Infraredfilter............................................................................
11. Motorized Focus axis..............................................................................
12. LN scope without Trinocular tube............................................................
13. Trinocular Tube.......................................................................................
14. Condenser...............................................................................................
15. Swing nosepiece.....................................................................................
16. Manual setting of Height with the Handwheel.........................................
17. Set the Height of the microscope head unit............................................
18. Lamp housing..........................................................................................
19. Mounting the Dodt-Grandient-Contrast (DGC).......................................
20. Frost Filter...............................................................................................
21. Mounting of the Reflected light fluorescence illuminator BC-RFA..........
22. Technical data.........................................................................................
3
1
2
3
4
5
6
7 / 8
9 / 10
11
12
13
14 /15 /16
17 / 18
19 / 20
21 / 22
23 / 24
25 / 26
27
28
29
30 /31 / 32
33 / 34 / 35

Special features for the new upright microscope from Luigs & Neumann
4.1 The LNscope microscope contains the following features:
- Superior optical imaging properties by using original microscope components
- Upgrade and retrofit of accessories from Olympus for the upright microscope BX51WI
- Suitable for microscopy tasks such as bright field, imaging, laser scanning up to
two-photon systems
- Motorized focus
- Motorized positioning of the condenser (optional)
- Flexible design of modular microscope body allows:
stepless height adjustment of objective and reflected light illuminator by
a manual Z drive allows working with different heights of probes and platforms
fast exchange device for condenser and condenser carrier for the use of in vivo and
in vitro platforms
easy removal of the transmission light device for even more space while,
for example, in vivo structures reality jet ball system or treadmill are used
- Sturdy construction with stainless steel guide columns, results in an
outstanding stability, thermal drift and vibration free characteristics.
- Slim design for the use of up to 16 manipulator units
- Ground connection for electrophysiological measurements
- Z-Drive compatible with LN controls (e.g. SM7, Mini units, Junior units)
4.2 Advantages over competitors
- The modular design of the microscope covers all task fields for which different
microscope types were needed in the past
InVitro (slice and hole cell measurements)
InVivo (imaging and measurements of anesthetized animals on platforms or
stereotaxic frame )
InVivo (imaging and measurements of wide-awake animals on stereotaxic frame,
Virtual reality jet ball systems, treadmills or mobile home cage system)
- Compatible with controls of LN manipulators. The customer can use one control for
Microscope and Manipulator
- Stepless height adjustment of objective and reflected light illuminator by
a manual Z drive for maximum flexibility in height
- Ground connection for electrophysiological measurements
- Customized designs are easy to implement and use available on request due to
the modular design
4. Instructions
4

5. Overview: LNscope
Condenser arm
Transmitted light unit
Microscope
main column
Hand wheel
for the manual
height adjustment
Clamping lever
Height adjustment
Focus unit, motorized
Frost filter
Nosepiece arm
Tubusplate
Microscope mount
Clamping screw
for the
microscope head Unit
Light shielding tube
5

Lamphouse
DGC
LNscope
microscope main body
incl. motorized focus-drive
Transmitted light unit
Condenser
carrier
manual / motorized
Condenser
Objective
Nosepiece
Tube-plate
Reflected light
Fluorescence illuminator
Trinocular tube
OLYMPUS
U-TLU
OLYMPUS
U-TV1x-2
OLYMPUS
U-CMAD3
Fourfold
changer
C-mount
1"
C-mount
0,5"
Camera
IR-Filter
6. LNscope-main body + accessories
6
Overview

7. Mount the LNscope onto the Breadboard
7.1 Fasten the LNscope-main body onto the breadboard
with the four screws
metric: 4x M6x40
inch : 4x 1/4" -20 UNC x1 1/2"
breadboard
LNscope main body
7

50mm
2"
100mm
4"
8

8. Mount the transmitted light unit
8.1 Place the transmitted light unit onto the microscope mount and
screw it tightly.
transmitted light unit
microscope mount
metric: M6x25
inch : 1/4"-20 UNC x1"
9

275mm
11"
50mm
2"
225mm
9"
10

9. Mounting the condenser-carrier
9.1 Slide the condenser-carrier onto the condenser-adapter and
fix it with the clamp-screw on the side
condenser carrier
condenser adapter
clamp screw
11
clamp screw

spring washer
Infra-red filter
FL-000 100
Holder
for
Infraredfilter
10. Holder for Infraredfilter
Holder for
Infraredfilter
12

11.2 Connect the motor cable of the motorized Focus axis onto
the appropriate LN-Controller SM10.
Connecting cable
motorized Focus axis
Focus axis
11. Motorized Focus-axis
11.1 The Focus axis can be moved finely and quickly over the LN-Keypad.
LN-Controller SM10
traverse path: 28mm
13

12. LN scope without Trinocular tube
Olympus
U-TLU
Olympus
U-TV1x-2
Olympus
U-CMAD3
LN fourfold
changer
C-mount
0,5" or 1"
Camera
14
Overview

12.1
12.2
12.3
12.4
U-TLU
U-TV1x2
15

12.7
Camera
12.5
U-CMAD3
12.6

Olympus
U-TV1x-2
Olympus
U-CMAD3
LN fourfold
changer
C-mount
0,5"
Camera
C-mount
1"
13. LNscope with Trinocular tube
17
Overview

13.1
13.2
18
Trinocular tube

14. Condenser
14.1 Slide the Condenser onto the Condenser carrier and
fix it with the clamping screw on the side
condenser
condenser carrier
clamp screw
19
Mounting the Condenser