Nikon LABOPHOT2-POL User manual

Thank you very much for
purchasing a Nikon Microscope.
This microscope is a high precision
instrument with a very delicate
structure and varied functions.
Please thoroughly read this manual
first to use the microscope correctly.
CAUTIONS
DAvoid Strong Shocks!
Handle the microscope gently. taking
care to avoid strong shocks.
fJWhen Carrying the Microscope
When carrying the microscope, support
the bottom of the microscope base. The
Instrument weighs about 7. 9kg. Do not
hold the overhanging portion of the base.
IiJPurpose
Use the microscope only for microscopIc
observations. Do not use It for any other
applications.
mPlace of Use
Avoid exposing the microscope to place
where it may be subject to dust,
vibrations, high temperatures, mOisture
or direct sunlight.
mlnstallation
Install the microscope apart from wall In
such a way that the lamp unit warning
label IS vIsible.
G)lnput Voltage
Confirm that the Input voltage to the mlcro~
scope corresponds to your line voltage.
BLight Source
Use a 6V~30W halogen lamp Do not
use lamps other than the one specified on
p.41 (Electrical Specifications). If a lamp
of more than the suggested wattage IS
used, the light adjusting circuit may be
damaged.
(;]When the Lamp Is On
Take care not to touch the lamp housing
or bring Inflammable substances such as
gasoline. thinner and alcohol near It, as
some parts of the lamp hOUSing become
very hot.
mChanging the Lamp-bulb
Before replaCing the Lamp~bulb, turn off
the main switch and disconnect the power
source plug. Before replaCing the
halogen lamp (6V~30W), be sure that It is
cool enough Do not touch the glass part
With
your bare hands.
mJDirt on the Lens
Do not leave dust, dirt or finger marks on
the lens or bulb surfaces. They will
prevent you from obserVing the specimen
clearly.
IDFocus Knobs
Never attempt to adjust the tightness of
the
rlght~ and left~hand focus knobs by
turning one while holding the other. It
may cause problems. Avoid forCing the
coarse focus knobs to turn past their
limits since that may cause problems.

CONTENTS
I .NOMENCLATURE ························1
II.ASSEMBLY····································4
III.MiCROSCOPy··························· 11
N .OPERATIONS IN DETAIL ......... 17
1 .Orientation of the Dia·polarizer ...... 17
2. Focusing and Centering the
Bertrand
Lens' ............................. 18
3. SWing Out the Top Lens of the
Condenser ................................. .. ·19
4.1 !4A & Tint Plate ........................ 19
5. Centering the Objectives ............... 20
6. Stage Rotation ....· .. ...................... ·20
7. Focusing .................................... 21
8. Centering the Condenser ............... 22
9. Use of Condenser Aperture
Diaphragm ................................. 23
10. Use of Field Diaphragm .............. ·24
11 .Optical Path Change·Over........· .. ·24
12 .Interpupillary DistanceAdjustment·25
13. Diopter Adjustment ..................... 25
14. Oil Immersion Manipulation ........ ·26
15. Brightness and Contrast
Adjustment ............................... 27
16. Objectives and Eyepieces ............ 29
V .OPTIONAL ACCESSORIES ...... 30
1. Senarmont Compensator .............. ·30
2. Quartz Wedge .............................. 31
3. Pin Hole Eyepiece ........................ 32
4. Attachable Mechanical Stage" ....... 32
5. Universal Epi-illuminator ............... 33
VI.TROUBLESHOOTING ............... 35
ELECTRICAL SPECIFICATIONS ... 41
CARE AND MAINTENANCE
DCleaning the Lenses
To clean the lens surfaces. remove dust
uSing a soft brush or gauze. Only when
removing finger marks or grease, use a
soft
cotton cloth, lens tissue, or gauze
lightly
moistened With pure alcohol
(methyl alcohol or ethyl alcohol). For
cleaning the objectives
of immersion oil
use only xylene. Do not use xylene for
cleaning the surface of the entrance lens
of the eyepiece tube or the prism surface
of the Ultra-Wide Eyepiece Tube "UW".
Observe sufficient caution In handling
alcohol and xylene (they are inflammable),
and the ON-OFF of the power source
sWitch.
fjCleaning the Painted Surfaces
Avoid the use of any organic solvent (for
example,
thinner, ether, alcohol) for
cleaning the painted surfaces and plastic
parts
of the instrument. We recommend
you use the Silicon cloth.
8lNever Attempt to Dismantle!
Never attempt to dismantle the
instrument because you may
impair the
functions.
mWhen Not in Use
When not in use, cover the Instrument
with the accessory vinyl cover, and store
it
in a place free from moisture and
fungus. It
is especially recommended
that the objectives and eyepieces be kept
In an airtight container containing
desiccant.
GiPeriodic Checking
To maintain the best performance of the
Instrument, we recommend that the
Instrument be periodically checked. (For
details
of this check, contact your
authOrIZed Nikon distributor.)
*Please note as per your Nikon warranty.
"Any defects or damage directly or
indirectly caused by the
use of
unauthorized replacement parts
and/or
performed by unauthorized personnel"
will void the warranty.
..
II

1
I • NOMENCLATURE
I . NOMENCLATURE
Intermediate tube
Nosepiece centering
screw
Centering revolving
nosepiece
CF Achromat P objective
(Strain-free)
Specimen clip
Circular graduated
stage
Stage
clamp screw
Condenser centering
screw
Condenser aperture
diaphragm control ring
Field
diaphragm
control ring
Power switch
Brightness adjuster
Binocular eyepiece tube
Eyepiece tube
clamp screw
Bertrand lens
centering screw
Analyzer knob
Intermediate tube
clamp screw
Revolving nosepiece
clamp screw
Coarse-focus torque
adjustment ring
Fine-focus scale
Lamp hOUSing
Arm rest
Fig.1-1

II. ASSEMBLY
II. ASSEMBLY
To assemble the microscope, follow the procedures given from
CD to @. For details, read p.5 to p. 10.
%@
a®---...6
t
~CV
@
~Ptionall
Fig.2
Tools: hexagon wrench (accessory), plus screwdriver, minus
screwdriver, coin, etc.

Bertrand lens
centering screw
Analyzer clamp screw
Analyzer rotation ring
Analyzer scale
Coarse
focus knob
Fine
focus knob
Power cord
Condenser focus
knob
Condenser clamp
screw
ND filter knob
I. NOMENCLATURE
Eyepiece
Diopter ring
Bertrand lens turret
Bertrand lens
focus ring
1/4'" & tint plate
Vernier
Stage rotation
clamp screw
Condenser top lens
swing~out knob
(Optional)
Fig.1-2
2

5
II. ASSEMBLY
CD Leveling Foot Screw
• For stable installation of the
microscope, manipulate the
adjustment screw at the
rear-right of the base
(Fig.2-1).
Fig.2-1
(2) Lamp and Lamp Housing
ICAUTIONS I
• Use the specified lamp "Nikon Halogen 6V 30W".
e Use the specified Halogen Lamp. See Electrical Specifications
on p.4 1 tor details .
• Keep the lamp cover on or use gloves when mounting the lamp
bulb, so as not to touch the surface directly with your fingers.
If the bulb surface IS contaminated with finger marks or dirt,
wipe them off with lens tissue. Be sure to remove the lamp
cover after mounting the lamp.
• Remove the lamp housing cover pressing the cover detaching
buttons (Fig. 2-2).
elnsert the lamp Into the socket pin holes until it reaches the limit
(Fig. 2-3).
• Reattach the cover.
Cautions when dismounting
• Turn oft the power switch. (Assure that It is off.) Do not touch the
lamp-bulb Immediately after turning It off because It is very hot.
Wait until it cools enough for replacing.
• Power supply cord should be kept away trom lamp housing while the
lamp hOUSing is hot.
Fig.2-2 Fig.2-3

II. ASSEMBLY
Q) Objectives
• Screw in the objectives to the holes of the revolving nosepiece in
such positions
that, when viewed from above, their magnifying
power increases as the nosepiece is revolved clockwise (Fig. 2-4).
Cautions when dismounting I
• Remove the NO filter cassette (Ex.)
If attached and lower the stage
by
turning the coarse focus
knob.
• Remove any specimen from
the stage.
• Hold the objective so as not to
drop it when unscrewing.
® Stage
• Lower the substage by turning the coarse focus knob.
• Loosen the stage clamp screw sufficiently.
Fig.2-4
• Fit the stage to the circular dovetail of the substage 00. Fasten the
stage with the stage clamp screw ~ (Fig. 2-5).
Fig.2-5 Fig.2-6
@ Specimen Clip
.Insert the two speciment clips into the holes on the stage surface
(Fig.
2-6).
® Condenser
• Raise the substage by turning the coarse focus knob.
• Lower the condenser carrier to its limit by turning the condenser
focus knob .
• Insert the condenser into the condenser carrier 00 with the numerical
aperture plate facing toward the user. Fasten it with the condenser
clamp screw ~ (Fig. 2-7).
• Raise the condenser carrier to its limit by turning the condenser
focus knob.
6

7
II. ASSEMBLY
Fig.2-7
(J) Dia-polarizer
Cautions when dismounting
• Remove the ND filter cassette
if attached .
• Lower the condenser carrier to
its
limit by turning the
condenser focus knob.
Remove the condenser by
releasing
the condenser clamp
screw.
• Fit the dia-polarizer into the bottom of the condensor (Fig. 2-8).
Fig.2-8 Fig.2-9
@ Intermediate Tube
• Mount the intermediate tube onto the microscope arm fitting the
notch of the circular devetail of the intermediate tube with the end of
the clamp screw. Tighten the clamp screw holding the mid-stem of
the hexgon wrench. Do not over tighten the screw holding the
L-shaped
holder of the wrench. (Fig. 2-9)
® 1/4A & Tint Plate
• Remove the screw from the side of the 1/4 A plate. Insert the plate
Into the compensator slot of the intermediate tube facing the
positioning notches toward the user.
Screw in the screw once removed (Fig. 2-10).
Fig.2-10
Fig.2-11

II. ASSEMBLY
@ Binocular Eyepiece Tube
e Mount the eyepiece tube onto the intermediate tube fitting the notch
of the circular dovetail of the eyepiece tube with the end of the
clamp screw. Tighten the clamp screw using the hexagon wrench.
(See @ and Fig.2-11 .)
ICautions when clamping I
eTighten the eyepiece tube clamp screw holding the stem of the
hexagonal wrench. If overtightened holding the plastic part,
optical-path change-over of the eyepiece tube may be
malfunctioned.
QJ) Eyepiece
e Use the same magnification eyepieces for both the right and left
eyes.
elnsert the eyepieces into the sleeves of the binocular eyepiece tube
by engaging the three grooves of the eyepiece with the three
protrusions of the sleeve (Fig. 2-12).
e When using eyeguard rubbers, put them onto the eyepieces
(Fig.2-13).
Fig.2-12
*Rotation clamp ring for the eyepiece
Fig.2-13
The rotation clamp ring is mounted on the eyepiece sleeve to
prevent the rotation of the eyepiece.
When removing the eyepiece, take care not to accidentally hold this
ring and pull it out together with the eyepiece.
Rotation clamp ring
for the eyepiece
Fig.2-14
8

9
II. ASSEMBLY
The rotation clamp ring must be removed when mounting the old
type of eyepiece. When attaching the ring again, note on the
following points.
uCaution when attaching rotation clamp ring
Hold the ring so that the ¢O.8 protrusion on the brim faces up.
There are two pawls on the ring and two grooves on the sleeve to
prevent the ring from failing off. Attach the ring so that these pawls
and grooves fits together.
O. 8-diameter protrusion
Pawls
Rotation clamp ring
for the eyepiece
Grooves
@ Filter on the Filter Receptacle
Binocular eyepiece
tube sleeve
Fig.2-15
Place the ¢45mm filter on the filter receptacle of the field lens part
(Fig. 2-16).
Fig.2-16
Fig.2-17
@ ND Filter Cassette (optional)
• Push down the ND filter cassette so the two protrusions of the
cassette bottom fit with the two mounting grooves on the rim of te
field lens part (Fig. 2-17) .
• When frequently lowering the stage, mount the ND filter cassette in
the direction as shown in Fig. 2-18 .
• When dismounting, push the cassette either to the left or right and
raise the opposite side.

II. ASSEMBLY
Fig.2-18 Fig.2-19
@ Power Cord
e Connect the socket of the power cord to the AC inlet on the rear of
the base (Fig. 2-19). Plug in the other end of the cord to an AC line
receptacle
with the ground conductor (earth conductor).
For 100-120Varea------------------------------~
e Use only the following power supply cord set.
UL listed, detachable cord set.
3-conductor grounding type SVT,
No. 18 AWG rated a minimum 1 25V, 7A.
el n case of using the extension cord, use only the power supply
cord including PE wire.
For220-240Varea------------------------------~
e Use only the 3-pole power supply cord type H05VV-F or
H05VVH2-F, which must be approved according to DIN VDE
0625. The plug and the outlet are to be approved according to
DIN VDE 0620 and DIN VDE 0625, respectively.
e Class I equipment should be connected to PE (protective earth)
terminal.
eln case of using the extension cord, use only the power supply
cord including PE wire.
@ Hexagon Wrench
e The hexagon wrench IS stored
in the back of the microscope
sta nd .
Take it
out as shown in
Flg.2-20.
Fig.2-20
10

11
III. MICROSCOPY
III. MICROSCOPY
Fig.3-1
Fig.3-2
-""' ... ----- .... ,
;,,; '\
" \
, '
f'MoTe ,
:~I
II~/
Fig.3-3
Fig.3-4
1) Put the NCB 11 filter (or
necessary filter) on
the filter
receptacle
of the field lens part
(Flg.3-1).
2) Mount the optional ND filter
cassette, if used (Fig. 3-2).
(Refer to II. ASSEMBLY- @
p.9.)
3) Turn on the power switch rn to
light the lamp.
Move the brightness adjuster
and align it with the 3rd scale
line from the right ~ (Fig. 3-3).
4) Pull the analyzer knob of the
intermediate tube rn to remove
the analyzer from the optical
path. Turn the Bertrand lens
turret to the position "0" ~ to
remove the Bertrand lens from
the optical path (Fig.3-4).

Fig.3-5
Fig.3-6
Fig.3-7
Fig.3-8
Ill. MICROSCOPY
5) Move the filter knobs of the ND
filter cassette
to the limit to
bring all ND filters into the
optical path (Fig.
3-5).
6) Revolve the revolving nosepiece
to swing in the 10 x objective to
the optical path. Assure that
the nosepiece properly settles in
position (Fig. 3-6).
7} Place the specimen (glass slide)
on the stage
with its cover glass
facing
up and fasten it with the
two specimen clips (Fig. 3-7).
8} Raise the condenser uppermost
using the condenser focus knob
(Fig.
3-8).
12

1Il. MICROSCOPY
13
Fig. 3-10
Binocular
part: 100%
.....
Fig.3-11
Fig.3-12
Fig.3-9
9) Fully open the
field and aperture
diaphragms
(Fig. 3-9).
10) Bring the object portion of
specimen into the optical path
(Fig. 3-1
0). (Object portion IS
just above the condenser
lens.)
11) When the trinocular eyepiece
tube
is used, change the
optical path
of the eyepiece
tube to enter 100% of the light
into the binocular part
(Fig.3-11).
(See p. 24, Operations in
Detail-11.)
12) Focus on the specimen
looking in the eyepiece by
manipulating the coarse/fine
focus knob (Fig.3-12).

Fig .3-13
Fig.3-14
Fig.3-15
Ill. MICROSCOP
13) Adjust the diopter (Fig. 3-13).
(See p. 25, Operations in
Detail-1 3.)
14) Adjust the interpupillary
distance (Fig. 3-1
4).
(See p. 25, Operations in
Detail-1 2.)
15) Center the obj.ective
(Fig.3-15).
(See p. 20, Operations in
Detail-5.)
14

15
III. MICROSCOPY
Image of field
diaphragm
Eyepiece
viewfield stop
Image of field
diaphragm
Eyepiece
~ viewfield stop
Fig.3-16
Fig.3-17
16) Focus and center the
condenser (Fig. 3-16).
(See p. 22, Operations In
Detall-8. )
17) Push In the analyzer knob to
put the analyzer Into the
optical path (Fig. 3-17).
18) Revolve the revolving
nosepiece to the objective to
be used and focus on the
specimen by manipulating the
fine or coarse focus knob.
(See Note: 2.)
When using an oil immersion
objective, apply oil to the
space between the top of the
objective and the specimen.
Take care not to produce
bubbles in the oil. (See p. 26,
Operations In Detail-14.)
19) When the ND filter cassette is
used, Adjust the brightness by
sliding the filter knob. (See
p. 27, Operations In
Detail-l 5.)
When the ND filter cassette is
not used, adjust the
brightness of the lamp by
manipulating the brightness
adjuster.
20) Adjust the viewfleld and the
aperture diaphragms by
manipulating their respective
control rings. (See p. 23 and
24, Operations In Detail-9
and -10.)

III. MICROSCOPY
Table 1
Orthoscopic microscopy
Conoscopic microscopy
10 x or IN
Top lens of higher
condenser
4x or
IN
lower
OUT
Bertrand lens OUT IN
lOx or
70%~80% of the
Circumscribed the
Aperture
higher
numerical aperture
circumference of the
of the objective
diaphragm
4x or
Fully opened
conoscoplc field of view
lower (or fully opened)
10x or
Circumscribed the
Circumference of the
Circumscribed the
higher
Field diaphragm
eyepiece field of view
circumference of the
4x or
lower
Fully opened orthoscopic field of view
Note 1 Manipulate the condenser centering screws if part of the
viewfield
is dark. If this doesn't help, check the following
items:
elnsertion/removal of the ND
filter
eTurning the revolving
nosepiece (click-stop position)
e Position of the condenser
eViewfield and aperture
diaphragms fully open
e Change-over of the optical
path of the eyepiece tube
e Mounting the lamp
e Mounting the condenser
e Placing the filter on the filter
receptacle
III. MICROSC;:OPY-5)
III. MICROSCOPY-6)
III. MICROSCOPY-B)
III. MICROSCOPy-g)
III. MICROSCOPY-ll)
II. ASSEMBLY-CZ)
Lamp and Lamp
Housing
II. ASSEMBLY-@
Condenser
II. ASSEMBLY-@
Filter on the Filter
Receptacle
Note 2 Check the following items when the specimen cannot be
focused.
e Placing the specimen III. MICROSCOPY-7)
eThickness of the cover glass (standard =0. 17mm)
16

17
N. OPERATIONS IN DETAIL (Operation part)
N. OPERATIONS IN DETAIL
1. Orientation of the Dia-polarizer
(Dia-polarizer and analyzer)
CD Place the specimen on the stage, focus on the specimen using the
objective 40 x .
® Set the analyzer scale on the intermediate tube to o.
G) Insert the dia~polarizer into the bottom of the condenser as shown in
Fig .4.
Dark cross image
Fig.4 Fig.5
® Remove the eyepiece from the sleeve or put the Bertrand lens into the
optical path. Observing the exit pupil of objective inside, rotate the
dia~polarizer so as to form a dark cross image on the exit pupil as
shown in Fig. 5.
eThe analyzer can be rotated by loosening the analyzer clamp screw rn
and rotating the rotation ring ~.
The rotation angle can be read in the range between O· to 180" with
the unit of 0.1· by the vernier.
The analyzer can be removed
from the optical path by pulling the
analyzer knob~. (Fig.6)
Note I As the depolarizer is built in the intermediate tube, you don't
need to be concerned about the relation between the
orientation of the polarizing plate and photomicrographic
device.
Fig.6

N. OPERATIONS IN DETAIL
2. Focusing and Centering the Bertrand Lens
(Bertrand lens of the intermediate tube)
• When objectives are replaced. turn the Bertrand lens focus ring under
the Bertrand lens turret and focus the Bertrand lens.
Centering is carried out uSing the condenser aperture diaphragm
Image and the two centering screws. (Fig. 7)
Centering procedure for the Bertrand lens is the same as that for the
condenser except that the condenser aperture diaphragm image is
used in place
of the field diaphragm image In condenser contering.
(Refer to Item 8.)
Note I Another Bertrand lens IS built in the optional tnnocular
eyepiece tube.
Turn the Bertrand lens nng to the position "B". and the
Bertrand lens will be brought into the optical path and a
conoscope image may be observed. (Fig. 8)
Fig.7 Fig.S
18