Nikon LABOPHOT2-POL User manual

Thank you very much for
purchasing a Nikon Microscope.
This microscope is a high precision
instrument with a very delicate
structure and varied functions.
Please thoroughly read this manual
first to use the microscope correctly.
CAUTIONS
DAvoid Strong Shocks!
Handle the microscope gently. taking
care to avoid strong shocks.
fJWhen Carrying the Microscope
When carrying the microscope, support
the bottom of the microscope base. The
Instrument weighs about 7. 9kg. Do not
hold the overhanging portion of the base.
IiJPurpose
Use the microscope only for microscopIc
observations. Do not use It for any other
applications.
mPlace of Use
Avoid exposing the microscope to place
where it may be subject to dust,
vibrations, high temperatures, mOisture
or direct sunlight.
mlnstallation
Install the microscope apart from wall In
such a way that the lamp unit warning
label IS vIsible.
G)lnput Voltage
Confirm that the Input voltage to the mlcro~
scope corresponds to your line voltage.
BLight Source
Use a 6V~30W halogen lamp Do not
use lamps other than the one specified on
p.41 (Electrical Specifications). If a lamp
of more than the suggested wattage IS
used, the light adjusting circuit may be
damaged.
(;]When the Lamp Is On
Take care not to touch the lamp housing
or bring Inflammable substances such as
gasoline. thinner and alcohol near It, as
some parts of the lamp hOUSing become
very hot.
mChanging the Lamp-bulb
Before replaCing the Lamp~bulb, turn off
the main switch and disconnect the power
source plug. Before replaCing the
halogen lamp (6V~30W), be sure that It is
cool enough Do not touch the glass part
With
your bare hands.
mJDirt on the Lens
Do not leave dust, dirt or finger marks on
the lens or bulb surfaces. They will
prevent you from obserVing the specimen
clearly.
IDFocus Knobs
Never attempt to adjust the tightness of
the
rlght~ and left~hand focus knobs by
turning one while holding the other. It
may cause problems. Avoid forCing the
coarse focus knobs to turn past their
limits since that may cause problems.

CONTENTS
I .NOMENCLATURE ························1
II.ASSEMBLY····································4
III.MiCROSCOPy··························· 11
N .OPERATIONS IN DETAIL ......... 17
1 .Orientation of the Dia·polarizer ...... 17
2. Focusing and Centering the
Bertrand
Lens' ............................. 18
3. SWing Out the Top Lens of the
Condenser ................................. .. ·19
4.1 !4A & Tint Plate ........................ 19
5. Centering the Objectives ............... 20
6. Stage Rotation ....· .. ...................... ·20
7. Focusing .................................... 21
8. Centering the Condenser ............... 22
9. Use of Condenser Aperture
Diaphragm ................................. 23
10. Use of Field Diaphragm .............. ·24
11 .Optical Path Change·Over........· .. ·24
12 .Interpupillary DistanceAdjustment·25
13. Diopter Adjustment ..................... 25
14. Oil Immersion Manipulation ........ ·26
15. Brightness and Contrast
Adjustment ............................... 27
16. Objectives and Eyepieces ............ 29
V .OPTIONAL ACCESSORIES ...... 30
1. Senarmont Compensator .............. ·30
2. Quartz Wedge .............................. 31
3. Pin Hole Eyepiece ........................ 32
4. Attachable Mechanical Stage" ....... 32
5. Universal Epi-illuminator ............... 33
VI.TROUBLESHOOTING ............... 35
ELECTRICAL SPECIFICATIONS ... 41
CARE AND MAINTENANCE
DCleaning the Lenses
To clean the lens surfaces. remove dust
uSing a soft brush or gauze. Only when
removing finger marks or grease, use a
soft
cotton cloth, lens tissue, or gauze
lightly
moistened With pure alcohol
(methyl alcohol or ethyl alcohol). For
cleaning the objectives
of immersion oil
use only xylene. Do not use xylene for
cleaning the surface of the entrance lens
of the eyepiece tube or the prism surface
of the Ultra-Wide Eyepiece Tube "UW".
Observe sufficient caution In handling
alcohol and xylene (they are inflammable),
and the ON-OFF of the power source
sWitch.
fjCleaning the Painted Surfaces
Avoid the use of any organic solvent (for
example,
thinner, ether, alcohol) for
cleaning the painted surfaces and plastic
parts
of the instrument. We recommend
you use the Silicon cloth.
8lNever Attempt to Dismantle!
Never attempt to dismantle the
instrument because you may
impair the
functions.
mWhen Not in Use
When not in use, cover the Instrument
with the accessory vinyl cover, and store
it
in a place free from moisture and
fungus. It
is especially recommended
that the objectives and eyepieces be kept
In an airtight container containing
desiccant.
GiPeriodic Checking
To maintain the best performance of the
Instrument, we recommend that the
Instrument be periodically checked. (For
details
of this check, contact your
authOrIZed Nikon distributor.)
*Please note as per your Nikon warranty.
"Any defects or damage directly or
indirectly caused by the
use of
unauthorized replacement parts
and/or
performed by unauthorized personnel"
will void the warranty.
..
II

1
I • NOMENCLATURE
I . NOMENCLATURE
Intermediate tube
Nosepiece centering
screw
Centering revolving
nosepiece
CF Achromat P objective
(Strain-free)
Specimen clip
Circular graduated
stage
Stage
clamp screw
Condenser centering
screw
Condenser aperture
diaphragm control ring
Field
diaphragm
control ring
Power switch
Brightness adjuster
Binocular eyepiece tube
Eyepiece tube
clamp screw
Bertrand lens
centering screw
Analyzer knob
Intermediate tube
clamp screw
Revolving nosepiece
clamp screw
Coarse-focus torque
adjustment ring
Fine-focus scale
Lamp hOUSing
Arm rest
Fig.1-1

II. ASSEMBLY
II. ASSEMBLY
To assemble the microscope, follow the procedures given from
CD to @. For details, read p.5 to p. 10.
%@
a®---...6
t
~CV
@
~Ptionall
Fig.2
Tools: hexagon wrench (accessory), plus screwdriver, minus
screwdriver, coin, etc.

Bertrand lens
centering screw
Analyzer clamp screw
Analyzer rotation ring
Analyzer scale
Coarse
focus knob
Fine
focus knob
Power cord
Condenser focus
knob
Condenser clamp
screw
ND filter knob
I. NOMENCLATURE
Eyepiece
Diopter ring
Bertrand lens turret
Bertrand lens
focus ring
1/4'" & tint plate
Vernier
Stage rotation
clamp screw
Condenser top lens
swing~out knob
(Optional)
Fig.1-2
2

5
II. ASSEMBLY
CD Leveling Foot Screw
• For stable installation of the
microscope, manipulate the
adjustment screw at the
rear-right of the base
(Fig.2-1).
Fig.2-1
(2) Lamp and Lamp Housing
ICAUTIONS I
• Use the specified lamp "Nikon Halogen 6V 30W".
e Use the specified Halogen Lamp. See Electrical Specifications
on p.4 1 tor details .
• Keep the lamp cover on or use gloves when mounting the lamp
bulb, so as not to touch the surface directly with your fingers.
If the bulb surface IS contaminated with finger marks or dirt,
wipe them off with lens tissue. Be sure to remove the lamp
cover after mounting the lamp.
• Remove the lamp housing cover pressing the cover detaching
buttons (Fig. 2-2).
elnsert the lamp Into the socket pin holes until it reaches the limit
(Fig. 2-3).
• Reattach the cover.
Cautions when dismounting
• Turn oft the power switch. (Assure that It is off.) Do not touch the
lamp-bulb Immediately after turning It off because It is very hot.
Wait until it cools enough for replacing.
• Power supply cord should be kept away trom lamp housing while the
lamp hOUSing is hot.
Fig.2-2 Fig.2-3

II. ASSEMBLY
Q) Objectives
• Screw in the objectives to the holes of the revolving nosepiece in
such positions
that, when viewed from above, their magnifying
power increases as the nosepiece is revolved clockwise (Fig. 2-4).
Cautions when dismounting I
• Remove the NO filter cassette (Ex.)
If attached and lower the stage
by
turning the coarse focus
knob.
• Remove any specimen from
the stage.
• Hold the objective so as not to
drop it when unscrewing.
® Stage
• Lower the substage by turning the coarse focus knob.
• Loosen the stage clamp screw sufficiently.
Fig.2-4
• Fit the stage to the circular dovetail of the substage 00. Fasten the
stage with the stage clamp screw ~ (Fig. 2-5).
Fig.2-5 Fig.2-6
@ Specimen Clip
.Insert the two speciment clips into the holes on the stage surface
(Fig.
2-6).
® Condenser
• Raise the substage by turning the coarse focus knob.
• Lower the condenser carrier to its limit by turning the condenser
focus knob .
• Insert the condenser into the condenser carrier 00 with the numerical
aperture plate facing toward the user. Fasten it with the condenser
clamp screw ~ (Fig. 2-7).
• Raise the condenser carrier to its limit by turning the condenser
focus knob.
6

7
II. ASSEMBLY
Fig.2-7
(J) Dia-polarizer
Cautions when dismounting
• Remove the ND filter cassette
if attached .
• Lower the condenser carrier to
its
limit by turning the
condenser focus knob.
Remove the condenser by
releasing
the condenser clamp
screw.
• Fit the dia-polarizer into the bottom of the condensor (Fig. 2-8).
Fig.2-8 Fig.2-9
@ Intermediate Tube
• Mount the intermediate tube onto the microscope arm fitting the
notch of the circular devetail of the intermediate tube with the end of
the clamp screw. Tighten the clamp screw holding the mid-stem of
the hexgon wrench. Do not over tighten the screw holding the
L-shaped
holder of the wrench. (Fig. 2-9)
® 1/4A & Tint Plate
• Remove the screw from the side of the 1/4 A plate. Insert the plate
Into the compensator slot of the intermediate tube facing the
positioning notches toward the user.
Screw in the screw once removed (Fig. 2-10).
Fig.2-10
Fig.2-11

II. ASSEMBLY
@ Binocular Eyepiece Tube
e Mount the eyepiece tube onto the intermediate tube fitting the notch
of the circular dovetail of the eyepiece tube with the end of the
clamp screw. Tighten the clamp screw using the hexagon wrench.
(See @ and Fig.2-11 .)
ICautions when clamping I
eTighten the eyepiece tube clamp screw holding the stem of the
hexagonal wrench. If overtightened holding the plastic part,
optical-path change-over of the eyepiece tube may be
malfunctioned.
QJ) Eyepiece
e Use the same magnification eyepieces for both the right and left
eyes.
elnsert the eyepieces into the sleeves of the binocular eyepiece tube
by engaging the three grooves of the eyepiece with the three
protrusions of the sleeve (Fig. 2-12).
e When using eyeguard rubbers, put them onto the eyepieces
(Fig.2-13).
Fig.2-12
*Rotation clamp ring for the eyepiece
Fig.2-13
The rotation clamp ring is mounted on the eyepiece sleeve to
prevent the rotation of the eyepiece.
When removing the eyepiece, take care not to accidentally hold this
ring and pull it out together with the eyepiece.
Rotation clamp ring
for the eyepiece
Fig.2-14
8