Accu-Scope EXC-120 SERIES User manual

73 Mall Drive, Commack, NY 11725 ● PH [phone] ● F [phone] ● [e-mail] ● www.accu-scope.com
EXC-120 Microscope Series
Phase Contrast Supplement
Alignment of the Phase Contrast Components
Underside View of Phase Turret,
Variable Aperture Diaphragm
and 4 Annuli Holders, Adjustable (X-Y)
Components
Phase contrast consists of two components for each phase
contrast objective installed on the microscope:
Phase Retarder Ring inside each Ph objective. This gray
Phase ring is observed using the focusable Phase Centering
Telescope supplied with the system. In the following
instruction you will be advised when to substitute the
telescope for one of the microscope’s eyepieces
Annulus Ring in the condenser. There is a different
annulus for each objective—one ring corresponding to each
Ph objective. Its purpose is to shape the incoming light into
a hollow cylinder. The annuli are mounted in holders that are
individually movable in an X-Y manner— so as to be able to
align the image of each annulus with the gray phase ring in
each objective.
Front Turret Installed and Positioned
Currently in Optic Axis
Installation
Install the Ph objectives on the microscope’s nosepiece
starting with the 10x, and then rotate the nosepiece clockwise
and install the 20x, 40x and 100xR oil objectives in numerical
order.
Install the Phase Contrast Condenser/Turret into the
condenser carrier mounting ring and tighten the set screw
with the screwdriver that came with your microscope.
NOTE: The condenser mounting ring has been pre-centered
at the factory.
Select the B for brightfield position on the Condenser/Turret
by rotating it clockwise until the B clicks into place as shown.
Rotate the nosepiece and bring the 10x objective into
position.
Raise the Condenser/Turret to its highest position.
Place a familiar “prepared, stained specimen” on the
stage and focus the 10x objective on this specimen.
10x Annulus
20x Annulus
40x Annulus
100x Annulus

73 Mall Drive, Commack, NY 11725 ● PH [phone] ● F [phone] ● [e-mail] ● www.accu-scope.com
Centering Telescope Correctly Installed
Alignment
To align, you need to superimpose the image of each
annulus onto its corresponding phase ring in each
objective by using the Centering Telescope as described
in the sequence below.
Replace one eyepiece with the Centering Telescope:
Remove one of the eyepieces by gently pulling it out of
the eyetube.
Insert the Centering Telescope into the eyetube.
1. Looking through the Centering Telescope, focus the
telescope on the gray 10x Ph phase ring in the objective
until the gray ring is sharp.
2.With the 10x objective in position, select the annulus
position that matches the 10x objective in use by rotating
the Condenser/Turret clockwise until the 10x annulus
position is in front and clicks into place.
3. Move the 10x annulus on the underside of the
Condenser/Turret to align the image of the annulus to the
phase ring in the objective as it appears in the diagram
below as (c).
Repeat the above procedure 1-3 for each Phase
contrast objective.
When alignment of all objectives is completed, replace
the Centering Telescope with the eyepiece and retighten
the set screw – be careful not to overtighten the set
screw.
Misaligned
to the left
Misaligned
to the bottom
CORRECTLY
ALIGNED