KERN OBN-14 User manual

OBN-14-BA-e-1510
KERN & Sohn GmbH
Ziegelei 1
D-72336 Balingen
E-mail: [email protected]
Tel: +49-[0]7433- 9933-0
Fax: +49-[0]7433-9933-149
Internet: www.kern-sohn.com
User instructions
Fluorescence microscope
KERN OBN-14
OBN 141, OBN 147, OBN 148
Version 1.0
01/2015
Importør:
Impex Produkter AS
Gamle Drammensvei 107
1363 Høvik
www.impex.no
[email protected]
Tel.: [phone]

OBN-14-BA-e-1510 2
GB
KERN OBN-14
Version 1.0 01/2015
User instructions
Fluorescence microscope
Table of contents
1Before use ....................................................................................3
1.1 General notes............................................................................................................................ 3
1.2 Notes on the electrical system................................................................................................ 3
1.3 Storage ...................................................................................................................................... 4
1.4 Maintenance and cleaning....................................................................................................... 5
2Nomenclature...............................................................................7
3Technical data / Features ............................................................9
4Assembly....................................................................................11
4.1 Microscope head .................................................................................................................... 12
4.2 Objectives ............................................................................................................................... 12
4.3 Eyepieces................................................................................................................................ 12
4.4 Colour filter ............................................................................................................................. 12
4.5 Condenser............................................................................................................................... 13
5Operation....................................................................................13
5.1 Getting started........................................................................................................................ 13
5.2 (Pre-) focussing ...................................................................................................................... 14
5.3 Adjusting the interpupillary distance................................................................................... 15
5.4 Dioptre adjustment................................................................................................................. 15
5.5 Adjusting the magnification.................................................................................................. 16
5.6 Adjusting the Koehler illumination....................................................................................... 17
5.7 Using eye cups ....................................................................................................................... 20
5.8 Using oil immersion objectives ............................................................................................ 21
5.9 Fluorescence reflected light unit.......................................................................................... 22
6Changing the bulb .....................................................................33
7Changing the fuse......................................................................33
8Using optional accessories.......................................................34
8.1 Polarisation unit ..................................................................................................................... 34
8.2 Camera connection ................................................................................................................ 35
8.3 Dark field units........................................................................................................................ 35
8.4 Phase-contrast units.............................................................................................................. 36
9Trouble shooting........................................................................39
10 Service........................................................................................41
11 Disposal......................................................................................41
12 Further information....................................................................41

3 OBN-14-BA-e-1510
1 Before use
1.1 General notes
You must open the packaging carefully, to make sure that none of the accessories in
the packaging fall on the floor and get broken.
In general, microscopes should always be handled carefully because they are
sensitive precision instruments. When using or transporting the microscope it is
particularly important to avoid abrupt movements, as this may damage the optical
components.
You should also avoid getting dirt or finger prints on the lens surface, because in
most cases this will reduce image clarity.
To maintain the performance of the microscope, it must never be disassembled. So
components such as lenses and other optical elements should be left as they were
before use. Also the electrical parts on the rear and base of the device must not be
tampered with, as in this area there is an additional risk of triggering an electric
shock.
1.2 Notes on the electrical system
Before connecting to a mains power supply, you must make sure that you are using
the correct input voltage. The information to select the correct mains cable is located
on the device, on the rear of the product directly above the connection socket. You
must comply with this information. If you do not comply with these specifications, then
fires or other damage to the device could occur.
The main switch must also be switched off before the mains cable is connected. In
this way you will avoid triggering an electric shock.
If you are using an extension cable, then the mains cable you use must be earthed.
If the original fuse should blow, it must only be replaced by an appropriate fuse.
Suitable replacement fuses are included with the delivery.
When carrying out any procedures whereby you come into contact with the electrical
system of the device, such as, for example, changing the bulb or fuse, only carry out
these procedures when the power is disconnected.

OBN-14-BA-e-1510 4
Under no circumstances should you touch the integrated halogen bulbs either during
operation or directly after use. These bulbs produce significant heat and therefore
there is a risk that the user could be severely burnt. So before handling the bulbs,
you must check that they have cooled down.
The microscope housing, on the other hand, is constructed so that no significant heat
radiation emanates from the device and that there is absolutely no risk of the user
burning themselves from the housing outer surfaces.
1.3 Storage
You should ensure that the device is not exposed to direct sunlight, temperatures
which are too high or too low, vibrations, dust or a high level of humidity.
The ideal temperature range is between 0 and 40°C and a relative humidity of 85%
should not be exceeded.
The device should always be located on a rigid, smooth, horizontal surface.
When the microscope is not being used, you should cover it with the enclosed dust
protective cover. When doing this, the power supply is stopped by switching off at the
main switch and unplugging the mains cable. If the eyepieces are being stored
separately, the protective caps must be fitted to the tube connectors. In most cases,
if dust and dirt gets inside the optical unit of a microscope this can cause irreversible
errors or damage.
The best way to store accessories which consist of optical elements, such as, for
example, eyepieces and objectives, is in a dry box with desiccant.

5 OBN-14-BA-e-1510
1.4 Maintenance and cleaning
In any event, the device must be kept clean and dusted regularly.
If any moisture should be occur, before you wipe down the device you must ensure
that the mains power is switched off.
When glass components become dirty, the best way to clean them is to wipe them
gently with a lint-free cloth.
To wipe oil stains or finger prints off the lens surface, moisten the lint free cloth with a
mixture of ether and alcohol (70 / 30 ratio) and use this to clean the lens.
You must be careful when handling ether and alcohol, as these are highly flammable
substances. You must therefore keep it away from naked flames and electrical
devices which can be switched on and off, and only use it in well-ventilated rooms.
However organic solutions of this type should not be used to clean other components
of the device. This could lead to damage to the paint finish. To do this, it is sufficient
to use a neutral cleaning product.
You could also use the following cleaning products to clean the optical components:
Special cleaner for optical lenses
Special optical cleaning cloths
Bellows
Brush
When handled correctly and checked regularly, the microscope should give many
years of efficient service.
Should repairs still be necessary, please contact your KERN dealer or our Technical
Department.

OBN-14-BA-e-1510 6
NOTE:
The Fluorescence microscopes of the series OBN-14
can be divided into two main components:
Compound microscope (KERN Professional Line)
Fluorescence reflected light unit
The following user manual refers at first merely to the
compound microscope.
The disquisition on the fluorescence reflected light unit is
carried out at the end of chapter 5. Operation.

7 OBN-14-BA-e-1510
2 Nomenclature
Eyepiece
Microscope head /
Tube
Adjusting screw
Microscope head
Nosepiece
Objective
Object holder
Specimen
stage
Aperture
adjustment
Condenser
Condenser
Field lens with
Field diaphragm
Control dial
X –Y axis
Specimen
stage
Fine
adjustment
knob
Coarse
adjustment
knob
Dimmer
Tube connectors with
dioptre adjustment ring
Centring screw
Condenser
Centring screw
Condenser
Adjusting screw
Condenser
Camera adapter connection
with adjusting screw
Trinocular toggle rod

OBN-14-BA-e-1510 8
Rear view
Adjusting ring
torque
Carrying
handle
Main switch
Power
connection
Coarse
adjustme
nt knob
Fine
adjustment
knob
Fuse
Condenser
Focus dial

9 OBN-14-BA-e-1510
3 Technical data / Features
Modell
KERN
Standard configuration
Optical system
Tube
Illumination
OBN 141
Infinity
Trinocular
LED + 3W-LED Epi Fluorescence (B/G)
OBN 147
Infinity
Trinocular
Halogen + 100W Epi Fluorescence (B/G)
OBN 148
Infinity
Trinocular
Halogen + 100W Epi Fluorescence (B/G/UV/V)
Eyepieces:
WF 10x / Ø 20 mm
Objectives:
4x / 10x / 20x / 40x / 100x
Product dimensions:
970x345x440 mm
Packaging dimensions:
530x220x490 mm
Net weight:
17 kg
Gross weight:
23 kg
Input voltage:
AC 100-240V, 50-60Hz
Output voltage:
DC 1.2-6V
Fuse:
2A 5x20 mm