Mascom MC513000 User manual

Operating Manual
GC-APPI Inferface for:
Orbitrap FusionTM TribridTM MS,
and Orbitrap FusionTM LumosTM
TribridTM MS
P/N: MC513000
Revision B

GC-APPI Interface Page 2
GC-APPI-Interface
© 2018 MasCom Technologies GmbH
Original Operating Instructions
The manual describesthe installation and
operation of the GC-APPI-Interface for the
Orbitrap Fusion seriesMS systems.
Revision History:
Revision A: published in December 2018
Revision B: published in May 2019
MasCom Technologies GmbH
Sophie-Germain-Str. 4
D-28201 Bremen
Germany
Tel.: [phone]
[email protected]
Trademarks
Fusion, Tribrid, Lumos, Orbitrap,
Thermo Scientific,Trace, Trace GC Ultra, and
Xcalibur are registered trademarks of Thermo
Fisher Scientific Inc. in the United States.

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Safety Instructions
Please read all ofthese instructions carefully
before starting the installation of theGC-APPI-
Interface and follow all recommendations.
1) Set themass spectrometerto “off”mode.
2) Cool down the heated capillary of the
mass spectrometer
3) When removing theESI sprayer be aware
thatthe heated capillary may still be hot.
4) Caution: don’t squeeze your fingers when
closing the cover of the interface
5) Please use nitrile gloves, or similar ones,
to ensureyour own safety and avoid
contamination.
6) Always unplug the heater cable before
detaching the interface from the mass
spectrometer. Never connect the
interface heatercables when the APPI
interface is not attached tothe mass
spectrometer. There is ahigh risk of over-
heating because the fanis not running.
Caution: hot surface with high risk to get burned!
During operation, the Interface housing may warm
up to 65 deg. C. When the interface is opened, the
heater is automatically turned off but thesource
may still be very hot (upto 330 deg. C, depending
on the operating temperature).
We also recommend watching the GC-APPI-
Interface installation video tofamiliarize yourself
with the procedure. This video has been provided
on a USB stick with theinterface. Alternatively,
you candownload it using the following link:
http://www.mascom-
bremen.de/html/en/downloads/gc-appi-
information

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Important information
Any changes or modification to the GC-APPI
interface, undertaken without our consent, may
endanger the functionality and/or safety, and
will result in the termination of the declaration
of conformity.
Tools required for source switching
Changing from ESI toGC-APPI on the Exactive or
LTQ Orbitrap series instruments only requires a
few tools:
Figure A: Screwdriver for removing the spray cone
and for tighten the plug on the APPI lamp
Figure B: 5/16-wrench for the dopant gas
connection
Figure C: 6 mm Allen screwdriver for locking and
unlocking the interface on the Orbitrap Fusion
systems

GC-APPI Interface Page 5
Figure D: Special tool for removing the transfer
tube on Obritrap Fusion only
Tools used for theGC column connection on the
GC injector are described in the GC manual.

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Installation of the GC-APPI Interface
Before installing the GC-APPI-Interface you have
to remove the ESI sprayer. Make sure the mass
spectrometer is set to “Off”, and the heated ion
transfer tubeis set to room temperature (see
Figure 1 and 4).
Caution: Removing the ESI sprayer while the
instrument is on or in standby will load the default
Tune file and eventually start the capillary heating.
Figure 1: Instrument in “Off”mode
Disconnect the waste hose from the bottom of the
TNG source,as well as all gas, syringe, and
electrical connections.
Figure 2: Instrument with ESI sprayer

GC-APPI Interface Page 7
Carefully removethe ESIsprayer from the mass
spectrometer interface andput it aside in a safe
place.
Remove the Thermo ion sweep coneas shown in
Figure 3, which is the removable spray shield that
sits just over theion transfer tube. Using a small
flat-head screwdriver, loosen the set screws on
the sides of the spray shield.
Caution: Theion sweep cone may still be hot.
Be sure to cooldown the ion transfer tube.
Figure 3: TNG source with sweep gas cone
Temperature setting is done on the TUNE page
(see Figure 4).
Figure 4: Ion transfer tube temperature setting
35

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The next step (Figure 5 and 6) is for
Orbitrap Fusion Lumos only:
Place the Vespel disk (P/N: MC502620) on the
original ion transfer tube(no need to change it as
on the OrbitrapFusion) with the rounded edge
towards the APPI-Interface (see Figure 6).
Figure 5: Ion source without sweep cone
Figure 6: Ion transfer tube with Vespel disk
The next steps (Figure 5x, 6x and 7x)
are for Orbitrap Fusion only:
After removing the ESI sprayer, you canaccess to
the transfer tube of the mass spectrometer.
Remove the transfer tubewith the special tool

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Figure 5x: Special tool for transfer tube removal
Figure 6x: Standard (A) and GC-APPI (B) transfer
tubes (P/N: MC501730)
(see Figure 5x) or with the 5/16wrench as
described in the operating manual of the mass
spectrometer.
Caution: Exchanging the transfer tubes at high
temperature is difficult and may damage them.
Replace the standard transfer tube (A) with the
GC-APPI transfer tube (B) (P/N: MC501730, see
Figure 6x,), and place the Vespel disk (P/N:
MC502610) on to the transfer tube(Figure 7x).
Make sure the disk is clean andfree of dust.
Figure 7x: GC-APPI transfer tube with Vespel disk
A
B

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All followings steps are for both Fusion type
systems.
Bring the closed GC-APPI Interface in frontof the
Mass Spectrometer and arrange the cable for the
APPI lamp close to its final position. Mount the
interface, open it and lock it (Figure 7a, 7b, 8aand
8b) with a6 mm Allen wrench.
Figure 7a: Locked position of the leftGC-APPI-
Interface connector
Figure 7b: Locked position of the rightGC-APPI-
Interface connector
Figure 8a: Mounting of the GC-APPI-Interface