Milton Roy Spectronic 501 Specifications

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WARM.UPlPRE.OPERATIONAL SEQUENCE
a. PluS power cord into appropriate power source
b. TLrn on spectrophotometer (move back panel toSgle switch to
the right).
c. The message "SELF TEST" will appear on the 501/601's display
while the instrument performs a pre-operation sellcheck.
d. Approximately twenty seconds after you switch the power on,
"SELFTIEST" will be replaced by the message "GOING TO Xxx
NM" (XXX = pre-programmed wavelength value), and then by
wavelength and data values.
NOTE
For effective operation, the spectrophotometer must warm up
for one-half hour after being turned on. Allow an additional l5
minutes for stabilization when you turn a lamp on or off.
Allow an additiooal 5 minutes for stabilization when switching
the LAMP SAVE function for the tungsten lamp on or off.
NOTE
Check the voltage label plate on the spectrophotometer's back
panelto determine what voltage (100, 115, 220, or 240) has been
set before you plug your SPECTRONIC 501 or 601 in. lf the
voltage shown on the label plate is not the same as the voltage of
your power source, do not plug the instrument in-call your
authorized lnl:-a Sales/Service representative to ar-
range for a voltage conversion.
s=__-/
b:&.
BACK PAI\IEL
SPECTRONIC
601
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KEYBOARD
SAMPLE COMPARTMENT

SUMMARY OF KEYBOARD FUNCTIONS
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oDisplay or set lower limit. If data falls
below this limit, "out-of-range" flag (an
asterisk "*") will appear on the display.
Displays or sets tungsten lamp save (on,/
off).
Display or set lamp interchange wave-
length (SPECTRONIC 601 only).
Display or set upper limit. If data ex-
ceeds limit, an "out-of-range" flag (an as-
terisk "*") will appear on the display.
Display or set signal averaging function.
'Iirrn Deuterium Lamp on or off (601
only).
Tirrn 'Iirngsten-Halogen Lamp on or olf
(601 only).
-
.Iry Set transmittance data to 100% or ABS
[ ,i'-111& .l El^,t data to o.oA.
Move monochromator up in wave-
length.
Move monochromator down in wave-
length.
NOTE
The < YES > and < NO > keys function
as "accept" or "reject" keys only when
the spectrophotometer is used in con-
junction with an APPLIPAK cartridge
and the Accessory Control Module (or
certain diagnostics).
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f,j----;ml @ Display or set concentration lactor'
@@ Access diagnostic tests.
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O @@ @ Numericentrykeys.
f,j-,-]ffil @ Edit current test parameters'
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f--"1-,1,x-] ( "*,o')
List current test parameters on display
(or printer).
Catalog list of stored tests on display (or
printer).
Choose a stored test from those included
in catalog.
NOTE
< SECOND FUNCTION > <EDIT> ...
< LtsT > < CATAI_OG >
<SELECT> are active only when the
Accessory Control Module is installed
and turned on.
lll
f,.-,-Hl A.tirrut" a key's second function.
Itgg- Send data to optional RS-232 interface.
I ser,ro I tor printer, Accessory Control Module.
or remote computer. @
(G-l D"l"t" "roneous data entry.
Set Tiansmittance, Absorbance, or Con-
centration data mode. f,i','-JFl@

This manual presents basic information on the use ofthe SPECTRONIC 501,/601 spectrophotometer 'lbrms contained with < >
are names of keys found in the keyboard or appropriate alphanumeric key entries.
LIMITED WARRANTY
SPECTRONIC instrumentation and related accessories are
warrantied against defects in materials and workmanship
for a period of (1) year from the date of delivery. This
warranty is provided only if the warranty registration
card is returned to Milton Roy within lilteen (15)days after
delivery.
This warranty covers all parts except those specified
below and applies only to equipment which has been
installed and operated in accordance with the operator's
instruction manual and which has been serviced only by
authorized Milton Roy dealers or service personnel. This
warranty does not apply to equipment and accessories
that have been damaged by accident, neglect or condi-
tions beyond Milton Roy's control.
This warranty does not apply to lamps, glassware and
similar expendable components. Howevel such parts and
components may be warranted by their manufacturer.
Milton Roy is not responsible under this warranty for
FCC COMPLIANCE STATEMENT FOR U.S.A. USERS
This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accord-
ance with the instruction manual, may cause interlerence
to radio communications. It has been tested aod found to
comply with the limits in effect at the time oI manulacture
for a Class A computing device pursuant to Subpart J of
Part 15 of FCC Rules, which are designed to provide rea-
loss in operating performance due to environmental
conditions.
THIS WARRANTY IS IN LIEU OF ALL WARRANTIES,
EXPRESS, IMPLIED OR STATUTORY INCLUDING, BUT
NOT LIMITED TO WARRANTIES OF FITNESS FOR A
PARTICULAR PURPOSE OR OF MERCHANTABILITY OR
OTHERWISE, and states Milton Roy's entire and exclusive
liability and the Customer's exclusive remedy for any
claim in connection with the sale or furnishing ol services,
goods or parts, their design, suitability for use, installa-
tion, or operations. The Milton Roy Company will in no
event be liable for any direct, indirect, special or con-
sequential damages whatsoever, including loss of good-
will, whether grounded in tort (including negligence),
strict liabiliiy or contract, and Milton Roy's liability under
no circumstances will exceed the contract price for the
goods and/or services for which liability is claimed.
sonable protection against such interference when oper-
ated in a commercial environment. Operation of this
equipment in a residential area is likely to cause inter-
ference in which case the user at his own expense will be
required to take whatever measures may be required to
correct the interference.
NOTE
This operator's instruction manual contains information, instructions and specifications that were believed accurate
at the time this manual was written. However, as part of our on-going program of product development, the specifi-
cations and operating instructions may be modified or changed from time-to-time. The Milton Roy Company
reserves the right to change such operating instructions and specifications. Under no circumstances shall Milton Roy
be obligated to notity purchasers of any future changes in either this operator's instructions manual or any other
instructions or specifications relating to the SPECTRONIC 501,2601 and their accessories nor shall Milton Roy be
liable in any way lor its failure to notify purchasers ol such changes.
Copyright O 1985, 1988 by Milton Roy Company.
All rights reserved.
SPECTRONIC and -€ are registered trademarks ol Milton Roy Company.

CONTENTS
SECTION
1
2
PAGE
OPERATOR FEATURES 2
INSTALLATION 3
2.1 Contents Check
2.2 Operational Check
KEYBOARD FUNCTIONS 4
DETAILED FEATURE DESCRIMION 6
4.1 High and Low Limits
4.2 Signal Averaging
4.3 'lirngsten Lamp
4.4 Lamp Selection (601 only)
4.5 Deuterium Lamp (601 only)
4.6 Lamp Interchange Wavelength (601 only)
OPERATION 8
5.1 Basic Spectrophotometric Measurements
5.2 Test Parameter Storage
MAINTENANCE & TROUBLESHOOTING 9
6.1 Performance Evaluation
6.2 Replacement Parts
6.3 Diagnostics
6.4 MaintenanceandTroubleshooting
6.4.1 Troubleshooting Chart
6.4.2 Error Messages
6.5 Lamp Replacement
6.5.1 Tirngsten-Halogen Lamp
6.5.2 Deuterium Lamp (601 only)
6.6 Lamp and Mirror Alignment
6.7 Fuse Replacement
RS-232.CINTERFACE_INTRODUCTION 15
R$232-C FIRMWARE SETUP PROCEDURE 17
R}232-C PROGRAMMING GUIDELINES 19
APPENDIX A
ALPHABETICAL LIST OF COMMANDS 21
APPENDIX B
PROGRAM EXAMPLES
APPENDIX C
KEYBOARD CODES
TITLE
3
4
7
8
9
22
335109100018

SECTION 1. OPERATOR FEATURES
FRONT
1. Sample Compartment Door.
2. Maintenance/Lamp Access Door provides access for
lamp replacement and alignment.
3. Sealed Keyboard controls instrument functions and
enters parameter data. Tactile and audible response indi-
cates data entry.
4. Lamp Save allows you to use the tungsten lamp at lower
illumination level (to extend lamp life).
5. Lamp Indicator shows "T" when Tirngsten-Halogen lamp
is on; "D" when deuterium lamp is oni and "8" when both
lamps are on.
6. 16-character Alphanumeric Display shows parameters
and data values, as well as operating and error messages.
SAMPLE COMPARTMENT
7. Cell Holder Base is removable for installation of acces-
sory sample holders, e.g., multipurpose cell holder, ambi-
ent flowcell, thermoelectric flowcell and automatic cell
positioner.
8. lOmm pathlength Cuvette Holder is removable for instal-
lation of accessory cell holders, e.g., thermal cell holders,
test-tube holder, and long pathlength cell holder.
9. Filter Holder holds filters up to 7mm thick.
10. Sample Compartment Windows.
11. Capped hole available for installation of accessory plug
connector.
BACK PANEL
12. Optional Recorder Interface connector.
13. Sample compartment accessory connector.
14. RS232 Interface connector-used for computer interface,
printer, and accessory control box.
15. Power Switch.
16. Line luse
17. Power cord
18. Maintenance/Lamp cover access screw
19. Exhaust fan
335101100018

SECTION 2, INSTALLATION
2.1 CONTENTS CHECK
After carefully unpacking the contents, check the mate-
rials against the packing list to ensure that you have
received everything in good condition.
If any part is missing or damaged, or you find any other
defect, please contact your dealer or Milton Roy sales
representative.
OPERAIIONAL CHECK
a. Set the unit on a clean lab bench or work area.
NOTE
Take care to allow enough clearance behind the spec-
trophotometer for efficient operation of the exhaust
fan.
b. Check the voltage label plate on the spectrophotom-
eter's back panel to determine what voltage (100,
I20,220, ot 240) has been set before you plug your
SPECTRONIC 501 or 601 in. If the voltage shown on
the label plate is not the same as the voltage of your
power source, do not plug the instrument in-call
your authorized Milton Roy Sales/Service represen-
tative to arrange for a vollage conversion.
c. If the power outlet is satisfactory, plug in the spec-
trophotometer's power cord.
d. TLrn power switch on.
e. Check to make sure that the "Power-On Sequence"
operates properly ('SELFifEST" message, followed
by "GOING TO - NM', and then by the normal
data display).
f . Allow thirty minutes for warm-up.
g. Check lamp alignment by performing the Lamp
Alignment Diagnostic (Section 6.6).

SECTION 3. KEYBOARD FUNCTIONS
< SECOND FUNCTION > activates a key's second func-
tion. The function, or its abbreviation, is printed just
above the individual key.
<SEND> transmits data to the optional serial port
accessory. (For a more detailed description, refer to
Sections 7 through l0 of this manual.) If the optional
printer, or an external computet is connected to the
RS-232C output on the rear panel of the instrument:
. Press < SEND > . The data on the display will be
transmitted to the computer or printer.
<SECOND FUNCTION> <SELECT> allows you to
load a stored test (requires the Accessory Control Mod-
ule and an APPLIPAK cartridge).
< CLEAR > restores a normal data display and is used
to exit several diagnostic tests.
<%T/A/C>:
a. Changes the data mode:
c Press <%oT / A/C > to switch from Absorbance to
Transmittance mode.
. Press <y"T/A/C> again to enter the Concen-
tration mode.
. Press <%T/A,/C> to return to the Absorbance
mode.
b. Can be used to enter an absorbance value. Make sure
that the spectrophotometer is in the absorbance
mode, then:
. Enter < appropriate value > .
. Ptess <YoT / A/C> .
. Display will read "NEW ABS ZERO."
. Display will show the wavelength and new absorb-
ance value.
The absorbance value that you enter will take the
place of any previous value. The difference between
the new value and the previous value is produced by
adjusting, or "offsetting" from zero (e.9., if your pre-
vious ABS value was 0.92, and you entered 0.97, the
value of zero will be adjusted, or offset, to +0.05).
c. Calculates a factor when you enter a concentra-
tion value standard solution:
. Make sure that spectrophotometer is in concen-
tration mode.
. Place solution of known concentration into the
sample compartment.
. Enter (appropriate value) for the concentration
value. The display will read ENTRY followed by
the value.
. Press < %T/A./C>.
. The display will read "CALCULATE FACTORJ' then
"FACTOR=," followed by the new value.
. The display will now return to the normal mode,
showing the wavelength and newly-entered con-
centration values.
The concentration value that you enter will be
divided by the current Absorbance value to calculate
a new factor between * 9999 and + 9999.
< SECOND FUNCTION > < 100'/"T /ZERO A> sets
transmittance to 100%, or absorbance or concentration
to zero.
3.7 <YES>:
a. Moves the monochromator up in wavelength.
b. Causes the monochromator to go to a user-specified
wavelength:
. Enter < appropriate value > .
. Press <YES>. The message GOING TO '.## NM
will be displayed until the monochromator is in the
correct position.
c. Accepts prompted values that appear during test
type programming (requires Accessory Control Mod-
ule and APPLIPAK cartridge).
d. Moves forward through a parameter list or EDIT list
(requires Accessory Control Module and APPLIPAK
cartridge).
3.8 <NO>:
a. Moves the monochromator down in wavelength.
b. Causes the monochromator to go to a user-specified
wavelength:
. Press < appropriate value>
. Press < NO > . The message GOING TO ### NM is
displayed until the monochromator is in the cor-
rect position.
c. Rejects prompted values that appear during test type
programming test type (requires Accessory Control
Module and APPLIPAK cartridge).
d. Move backward through a parameter list or EDIT list
(requires Accessory Control Module and APPLIPAK
cartridge).
3.9 < SECOND FUNCTION> <cO TO X> causes the
monochromator to go to a user-specified wavelength:
. Enter <appropriate value>. Display will show EN-
TRY value.
. Press <SECOND FUNCTION>.The display will now
read "2NDl' followed by the value.
. Press <GO TO tr>. The message-GOING TO ###
NM- will be displayed until the monochromator is in
the correct position.
3.10 <.> <t> and <0> through <9> are used to
enter positive and negative decimal and integer values.
3.11 < SECOND FUNCTION> <EDIT> changes any of
the prompted parameters of the current test (requires
Accessory Control Module and APPLIPAK cartridge).
3.12 <SECOND FUNCTION> <LIST> displays the para-
meters and their values for the current test (requires
the Accessory Control Module and APPLIPAK car-
tridge).
3.13 <SECOND FUNCTION> < CATALOG > lists the cata-
log numbers and names of user-stored tests (requires
the Accessory Control Module and an APPLIPAK car-
tridge).
3.I4 < SECOND FUNCTION> <FACTOR>:
a. Set a known factor for use with the concentration
data mode:
. Enter (appropriate value). The display will read
ENTRY followed by the value.
r Press < SECOND FUNCTION > . The display will
read 2ND, followed by the value.
o Press <FACTOR>. This value will be used to con-
vert absorbance data into concentration units.
3.4
3.6
335103.100018

b. Display the current factor
o Press < SECOND FUNCTION > . The display witl
read 2ND.
o Press < FACTOR>.
3.15 <SECOND FUNCTION > <HI LIM> displays or sets
\ the highesl acceptable final result. Any result above the
iimil will be flagged as out-of-limits (see Section 4.1,
High and Low Limits).
3.16 <SECOND FUNCTION > <LO LIM> displays or sets
the lowest acceptable final result. Any result below the
limit will be flagged as out-of-limits (see Section 4.1,
High and Low Limits).
3.17 <SECOND FUNCTION> <DIAG> accesses the inter-
nal diagnostics. See Section 6.3, Diagnostics.
3.18 <SECOND FUNCTION > <SIG AV> displays or sets
the signal averaging value (see Section 4.2, Signal
Averaging).
3.19 < SECOND FUNCTION > <LMPSAV> turns the tung-
sten lamp save on and off (see Section 4.3, Tungsten
Lamp).
. Enter 0, <SECOND FUNCTION > <LMP SAV> to
turn off the lamp save mode.
. Enter l, <SECOND FUNCTION > <LMP SAV> to
turn on the lamp save mode.
. Press < SECOND FUNCTION > < LMP SAV > to dis-
play the status.
3.20 <SECOND FUNCTION> <TUNG> turns the tung-
sten lamp on or off (601 only).
3.21 <SECOND FUNCTION> <DEUT> turns the deu-
terium lamp on or off (for SPECTRONIC 601 only).
The current lamp status is indicated by the last charac-
ter on the right of the display.
. "T" indicates that the tungsten lamp is on.
. "D" indicates that the deuterium lamp is on.
. "B" indicates that the both lamps are on.
NOTE
If you attempt to:
1. turn the deuterium lamp off when the unit is below
the lamp interchange wavelength, or
2. turn the tungsten-halogen lamp off when the unit is
above the lamp interchange wavelength . . .
the message LAMP REQUIRED will be temporarily dis-
played, and the normal data display will return. This
cannot be over-ridden by continually pressing the key.
To turn both lamps off, enter <1> < SECOND
FUNCTION> <DIAG>. The lamps will remain off,
and the display will read "STANDBY . . .", until you
press another key to return to the normal data display.
3.22 <SECOND FUNCTION> <LMPCH> displays or sets
the lamp interchange wavelength (see Section 4.6,
Lamp Interchange Wavelength). (SPECTRONIC 601
only).
335103-100014