NanoEnTek JuLi Stage User manual

2 JuLI™ Stage –User Manual
All the materials in this user manual are protected by Korean and International copyright laws.
They cannot be reproduced, translated, published or distributed without the permission of the
copyright owner.
JuLI™Stage User Manual
Website: www.nanoentek.com
E-mail: [e-mail]
Manufactured by
NanoEntek, Inc.
851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea
Tel: [phone]
Fax: [phone]
NanoEntek America, Inc.
220 Bear Hill Road, Suite 102, Waltham, MA 02451, USA
Tel: [phone]
Fax: [phone]
The information in this user manual is described as accurately as possible.
Firmware and software changes and updates may change without prior consent or notification.
Copyright © 2014 by NanoEntek, Inc.
All right reserved. Published in Korea
Documentation: NESMU-JST-001E
Revision history:
V.0.0 OCT 2014
V.0.5 MAY 2015
V.1.0 SEP 2015
V.1.5 MAR 2016
V.2.0 MAY 2017
V.2.5 MAR 2023

Contents
JuLI™ Stage - User Manual 3
1Safety and compliance .............................................................................................5
1.1 General safety precautions .........................................................................................5
1.2 Safety symbols............................................................................................................6
1.3 Intended use...............................................................................................................7
2Product overview......................................................................................................8
2.1 Scope of delivery ........................................................................................................9
3Description..............................................................................................................10
3.1 Front view.................................................................................................................10
3.2 Side view..................................................................................................................11
3.3 Rear view..................................................................................................................11
3.4 Control box ...............................................................................................................12
4Installation...............................................................................................................13
4.1 Unpacking the instrument..........................................................................................13
4.2 Installing the instrument............................................................................................14
4.3 Operating environment..............................................................................................17
4.4 Cautions ...................................................................................................................17
5User interface..........................................................................................................20
6Basic operation.......................................................................................................22
6.1 Moving......................................................................................................................22
6.2 Selection...............................................................................................................24
6.3 Position zoom ...........................................................................................................26
6.4 Mouse wheel.............................................................................................................27
7Operation.................................................................................................................29
7.1 Preview menu...........................................................................................................29
7.2 Setup menu ..............................................................................................................39
7.3 Data menu................................................................................................................52
7.4 Settings menu...........................................................................................................55
7.5 Quick Workflow (setting up an experiment)...............................................................59
8Cleaning and Maintenance.....................................................................................60
9Troubleshooting......................................................................................................62
10 Specifications..........................................................................................................63
10.1 Product specifications...............................................................................................63
10.2 Ordering information .................................................................................................64
10.3 Warranty...................................................................................................................65
11 JuLI™Stage EDIT Software.....................................................................................66
11.1 Software overview ....................................................................................................66
11.2 Operation..................................................................................................................68
11.2.1 Main viewer................................................................................................69
11.2.2 Image editor...............................................................................................75

Contents
4 JuLI™ Stage –User Manual
11.2.3 Movie Maker...............................................................................................78
11.2.4 Movie Maker –Single.................................................................................79
11.2.5 Movie Maker –Sequence...........................................................................84
11.2.6 Movie Maker –Matrix.................................................................................88
12 JuLI™Stage STAT Software....................................................................................90
12.1 Software overview.....................................................................................................90
12.2 Operation..................................................................................................................92
12.2.1 Main viewer................................................................................................93
12.2.2 Analysis modules .......................................................................................99
12.2.2.1 Growth Curve...........................................................................100
12.2.2.2 Scratch Basic...........................................................................125
12.2.2.3 Attached Cell Counting.............................................................128
12.2.2.4 Whole Intensity Level...............................................................133
12.2.3 Plate Editor ..............................................................................................138

Safety and compliance
JuLI™ Stage - User Manual 5
1 Safety and compliance
1.1 General safety precautions
If water or other material enters the instrument, adaptor, or power inlet,
disconnect the power cord and contact a service person. For operating
environment, refer to Product Specifications.
Do not touch the main plug or power cord with wet hands.
Always ensure that the power supply input voltage matches the voltage
available at your location.
This instrument is air-cooled and its surfaces may become hot during operation.
During installing, leave a space of more than 10 cm (4 inches) around the
instrument and do not place any objects between the instrument and the walls.
Do not install the instrument on a slant or a place prone to vibrations, which
induces the risk of instrument malfunction or damage of the instrument.
Never insert any objects into the air vents of the instrument as this could result
in electrical shock, personal injury, and equipment damage.
Plug the power cord firmly into the wall outlet and AC adapter.
To avoid potential shock hazard, make sure that the power cord is properly
grounded.
Be sure to position the equipment such that it is easy to disconnect.
Turn off the instrument before unplugging the power cord and/or moving the
instrument.
If the instrument is dropped or broken, disconnect the power cord and contact
a service person. Disassembling the case will void the warranty.
Use only authorized accessories (adaptor, power cord, and USB drive).

Safety and compliance
6 JuLITM Stage - User Manual
1.2 Safety symbols
Symbol
Meaning
Caution and Warning
Protective earth (Ground)
This instrument and consumables conforms to the EC
Declaration of Conformity.
This equipment has been tested and found to comply with
the limits for a Class A digital instrument, pursuant to Part
15 of the FCC Rules.
These limits are designed to provide reasonable
protection against harmful interference when the
equipment is operated in a commercial environment.
This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in
accordance with the instruction manual, may cause
harmful interference to radio communications. Operation
of this equipment in a residential area is likely to cause
harmful interference in which case the user will be
required to correct the interference at his own expense.

Safety and compliance
JuLI™ Stage - User Manual 7
1.3 Intended use
Warning! Use only as intended.
The JuLI™Stage system, including the original accessories may only be used in
conjunction with the methods described in this manual. NanoEntek does not
assume any liability for any other applications or procedures, including use of
individual subassemblies or components for other purposes. The manufacturer
does not assume any liability for any other kind of application, including individual
subassemblies and the addition of individual components. This exclusion of liability
also applies to all service or repair work which was not carried out by authorized
NanoEntek service personnel.
For research use only –not for use in diagnostic procedures
The JuLI™ Stage is a real-time live cell imaging system suitable for use in research
applications such as academic, pre-clinical, and for forensic applications only. Due
to its technical design the JuLI™ Stage is particularly suitable for cellular
applications.
Exclusion of Liability for Measurement Results
NanoEntek does not assume any liability for the correctness of measurement
results obtained with the NanoEntek JuLI™ Stagesystem nor for conclusions based
on these measurement results.

Product overview
8 JuLITM Stage - User Manual
2 Product overview
The JuLI™Stage is a real-time live cell imaging system that can acquire extended
image series directly from various cell culture plates (6 to 384 well), flasks, slides
and dishes in an incubator. The JuLI™ Stage supports a brightfield and three
fluorescence channels, three objective lenses, and sensitive filter-based optics to
optimize for various live cell assays. It combines ease-of-use with versatility by
offering simple workflows and robust analysis of different readout parameters.
The JuLI™ Stage is suitable for a wide variety of applications:
Live cell imaging (time lapse)
Cell culture quality control
Cell culture optimization
Cell-based assay optimization
Angiogenesis
Scratch-wound assay
Neurite growth
Protein expression
Kinetic imaging and cell differentiation
Cell growth monitoring
Stem cell development
Cell proliferation
Cytotoxicity
Cell migration

Product overview
JuLI™ Stage - User Manual 9
2.1 Scope of delivery
The JuLI™ Stage real-time live cell imaging system is shipped with the following
components.
Upon receiving the instrument, please check that all items listed below are included
in the shipment. If any part is missing or damaged, contact your local distributor or
[e-mail].

Description
10 JuLITM Stage - User Manual
3 Description
3.1 Front view
Cover
Used to protect test samples from external light. Simply put on the cover if you use
the JuLI™ Stage on the bench and/or look at light sensitive samples.
Main body
The main body contains all of the mechanical, electrical, and optical parts.