NanoEnTek JuLi Stage User manual


2 JuLI™ Stage –User Manual
All the materials in this user manual are protected by Korean and International copyright laws.
They cannot be reproduced, translated, published or distributed without the permission of the
copyright owner.
JuLI™Stage User Manual
Website: www.nanoentek.com
E-mail: sales@nanoentek.com
Manufactured by
NanoEntek, Inc.
851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea
Tel: +82-2-6220-7940
Fax: +82-2-6220-7999
NanoEntek America, Inc.
220 Bear Hill Road, Suite 102, Waltham, MA 02451, USA
Tel: +1-781-472-2558
Fax: +1-781-790-5649
The information in this user manual is described as accurately as possible.
Firmware and software changes and updates may change without prior consent or notification.
Copyright © 2014 by NanoEntek, Inc.
All right reserved. Published in Korea
Documentation: NESMU-JST-001E
Revision history:
V.0.0 OCT 2014
V.0.5 MAY 2015
V.1.0 SEP 2015
V.1.5 MAR 2016
V.2.0 MAY 2017
V.2.5 MAR 2023

Contents
JuLI™ Stage - User Manual 3
1Safety and compliance .............................................................................................5
1.1 General safety precautions .........................................................................................5
1.2 Safety symbols............................................................................................................6
1.3 Intended use...............................................................................................................7
2Product overview......................................................................................................8
2.1 Scope of delivery ........................................................................................................9
3Description..............................................................................................................10
3.1 Front view.................................................................................................................10
3.2 Side view..................................................................................................................11
3.3 Rear view..................................................................................................................11
3.4 Control box ...............................................................................................................12
4Installation...............................................................................................................13
4.1 Unpacking the instrument..........................................................................................13
4.2 Installing the instrument............................................................................................14
4.3 Operating environment..............................................................................................17
4.4 Cautions ...................................................................................................................17
5User interface..........................................................................................................20
6Basic operation.......................................................................................................22
6.1 Moving......................................................................................................................22
6.2 Selection...............................................................................................................24
6.3 Position zoom ...........................................................................................................26
6.4 Mouse wheel.............................................................................................................27
7Operation.................................................................................................................29
7.1 Preview menu...........................................................................................................29
7.2 Setup menu ..............................................................................................................39
7.3 Data menu................................................................................................................52
7.4 Settings menu...........................................................................................................55
7.5 Quick Workflow (setting up an experiment)...............................................................59
8Cleaning and Maintenance.....................................................................................60
9Troubleshooting......................................................................................................62
10 Specifications..........................................................................................................63
10.1 Product specifications...............................................................................................63
10.2 Ordering information .................................................................................................64
10.3 Warranty...................................................................................................................65
11 JuLI™Stage EDIT Software.....................................................................................66
11.1 Software overview ....................................................................................................66
11.2 Operation..................................................................................................................68
11.2.1 Main viewer................................................................................................69
11.2.2 Image editor...............................................................................................75

Contents
4 JuLI™ Stage –User Manual
11.2.3 Movie Maker...............................................................................................78
11.2.4 Movie Maker –Single.................................................................................79
11.2.5 Movie Maker –Sequence...........................................................................84
11.2.6 Movie Maker –Matrix.................................................................................88
12 JuLI™Stage STAT Software....................................................................................90
12.1 Software overview.....................................................................................................90
12.2 Operation..................................................................................................................92
12.2.1 Main viewer................................................................................................93
12.2.2 Analysis modules .......................................................................................99
12.2.2.1 Growth Curve...........................................................................100
12.2.2.2 Scratch Basic...........................................................................125
12.2.2.3 Attached Cell Counting.............................................................128
12.2.2.4 Whole Intensity Level...............................................................133
12.2.3 Plate Editor ..............................................................................................138

Safety and compliance
JuLI™ Stage - User Manual 5
1 Safety and compliance
1.1 General safety precautions
If water or other material enters the instrument, adaptor, or power inlet,
disconnect the power cord and contact a service person. For operating
environment, refer to Product Specifications.
Do not touch the main plug or power cord with wet hands.
Always ensure that the power supply input voltage matches the voltage
available at your location.
This instrument is air-cooled and its surfaces may become hot during operation.
During installing, leave a space of more than 10 cm (4 inches) around the
instrument and do not place any objects between the instrument and the walls.
Do not install the instrument on a slant or a place prone to vibrations, which
induces the risk of instrument malfunction or damage of the instrument.
Never insert any objects into the air vents of the instrument as this could result
in electrical shock, personal injury, and equipment damage.
Plug the power cord firmly into the wall outlet and AC adapter.
To avoid potential shock hazard, make sure that the power cord is properly
grounded.
Be sure to position the equipment such that it is easy to disconnect.
Turn off the instrument before unplugging the power cord and/or moving the
instrument.
If the instrument is dropped or broken, disconnect the power cord and contact
a service person. Disassembling the case will void the warranty.
Use only authorized accessories (adaptor, power cord, and USB drive).

Safety and compliance
6 JuLITM Stage - User Manual
1.2 Safety symbols
Symbol
Meaning
Caution and Warning
Protective earth (Ground)
This instrument and consumables conforms to the EC
Declaration of Conformity.
This equipment has been tested and found to comply with
the limits for a Class A digital instrument, pursuant to Part
15 of the FCC Rules.
These limits are designed to provide reasonable
protection against harmful interference when the
equipment is operated in a commercial environment.
This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in
accordance with the instruction manual, may cause
harmful interference to radio communications. Operation
of this equipment in a residential area is likely to cause
harmful interference in which case the user will be
required to correct the interference at his own expense.

Safety and compliance
JuLI™ Stage - User Manual 7
1.3 Intended use
Warning! Use only as intended.
The JuLI™Stage system, including the original accessories may only be used in
conjunction with the methods described in this manual. NanoEntek does not
assume any liability for any other applications or procedures, including use of
individual subassemblies or components for other purposes. The manufacturer
does not assume any liability for any other kind of application, including individual
subassemblies and the addition of individual components. This exclusion of liability
also applies to all service or repair work which was not carried out by authorized
NanoEntek service personnel.
For research use only –not for use in diagnostic procedures
The JuLI™ Stage is a real-time live cell imaging system suitable for use in research
applications such as academic, pre-clinical, and for forensic applications only. Due
to its technical design the JuLI™ Stage is particularly suitable for cellular
applications.
Exclusion of Liability for Measurement Results
NanoEntek does not assume any liability for the correctness of measurement
results obtained with the NanoEntek JuLI™ Stagesystem nor for conclusions based
on these measurement results.

Product overview
8 JuLITM Stage - User Manual
2 Product overview
The JuLI™Stage is a real-time live cell imaging system that can acquire extended
image series directly from various cell culture plates (6 to 384 well), flasks, slides
and dishes in an incubator. The JuLI™ Stage supports a brightfield and three
fluorescence channels, three objective lenses, and sensitive filter-based optics to
optimize for various live cell assays. It combines ease-of-use with versatility by
offering simple workflows and robust analysis of different readout parameters.
The JuLI™ Stage is suitable for a wide variety of applications:
Live cell imaging (time lapse)
Cell culture quality control
Cell culture optimization
Cell-based assay optimization
Angiogenesis
Scratch-wound assay
Neurite growth
Protein expression
Kinetic imaging and cell differentiation
Cell growth monitoring
Stem cell development
Cell proliferation
Cytotoxicity
Cell migration

Product overview
JuLI™ Stage - User Manual 9
2.1 Scope of delivery
The JuLI™ Stage real-time live cell imaging system is shipped with the following
components.
Upon receiving the instrument, please check that all items listed below are included
in the shipment. If any part is missing or damaged, contact your local distributor or
sales@nanoentek.com.

Description
10 JuLITM Stage - User Manual
3 Description
3.1 Front view
Cover
Used to protect test samples from external light. Simply put on the cover if you use
the JuLI™ Stage on the bench and/or look at light sensitive samples.
Main body
The main body contains all of the mechanical, electrical, and optical parts.

Description
JuLI™ Stage - User Manual 11
3.2 Side view
LED arm
The arm above the sample holds the LED for the brightfield illumination.
Automatic XY-stage
The automatic XY-stage precisely navigates to the intended position. The holder is
manufactured after SBS standard so that you can insert all standard microplates (6
to 384 well plates). Using one of the JuLI™ Stage vessel holders you can image
petri dishes, culture flasks, or glass slides.
3.3 Rear view
Cooling slits
The JuLI™ Stage ventilates the internal parts using 2 fans behind the slits on the
back side. Please make sure that the cooling system can operate freely.
Connection port (device)
Here you can connect the JuLI™ Stagewith the control box via the white connection
cable (see chapter 2 “Product overview”).
Power inlet
Connect the instrument to an electrical outlet usingthe supplied power cord and the
appropriate plug, based on the electrical outlet configuration in your country.

Description
12 JuLITM Stage - User Manual
3.4 Control box
Serial and camera USB cables
The control box can be connected to the PC by connecting the serial and camera
USB cables to the USB repeaters. When properly connected the serial and camera
LEDs are on.
Connection port (control box)
Via the white connection cable the JuLI™ Stagedevice and the control box can be
connected.

Installation
JuLI™ Stage - User Manual 13
4 Installation
4.1 Unpacking the instrument
1. Open the box and remove the material foam from the box.
2. Carefully lift the instrument out of the box, holding it by the handholds in the
bottom of the base.
3. Place the instrument on a flat, levelled surface (table or incubator).
IMPORTANT!
Do not lift the JuLI™ Stage using the LED arm.

Installation
14 JuLITM Stage - User Manual
4.2 Installing the instrument
Installation: Please make sure to carefully follow the installation instructions
in this manual in order to ensure safe operation. The final installation should
look as shown in the following connection overview:
PC ►USB-Repeater ►USB-Cables ►Control Box ►Connection Cable ►JuLI™ Stage
Make sure no cable is under tension.
1. Connect the PC with the repeater cables:
Connect the two repeater cables to the USB3 ports on the back of the PC.
Connect the PC to the power outlet using the power cord and turn on the
PC.
The blue light on the repeater cables should be on.

Installation
JuLI™ Stage - User Manual 15
2. Connect the other end of the repeater cables to the USB cables of the
control box.
The LEDs on the control box should be red. Protect the cable connection
with the plastic cover.
3. Plug the white connecting cable into the control box using the end of the cable
without the ferrite core.
4. Connect the other end of the connection cable with ferrite core to the JuLI™
Stage. Make sure that the connection cable is tightly connected to the
connection port. Then connect the power cord.
☞Note: Make sure that the JuLI™ Stage has enough space for the cables
and proper ventilation on the back side (~ 10cm).

Installation
16 JuLITM Stage - User Manual
5. Turn on the JuLI™ Stage using the switch on the control box.
6. Start the JuLI™ Stage control software and follow the warm-up guidelines.
First use: Before operating the JuLI™ Stagesystem for the first time, we strongly
recommend allowing the instrument to equilibrate to room temperature for
20 minutes outside the incubator while the device is turned on.
If the JuLI™ Stage is to be operated inside an incubator, an additional warm-up
time of 2 hours is required to equilibrate it to 37 °C. During this time the JuLI™
Stage must be switched on.
IMPORTANT!
When installing the JuLI™ Stage in the incubator, avoid the connection cables to be
under any tension. Only the JuLI™ Stage itself may be placed in the incubator, but
not the control box or other system components.

Installation
JuLI™ Stage - User Manual 17
IMPORTANT!
Use the cover if the instrument operates on the table to protect test samples from
external light.
4.3 Operating environment
Allow at least 10 cm (4 inches) free space at the back of the instrument to allow
for proper ventilation and prevent overheating of electronic components.
Set up the JuLI™ Stage away from direct light sources, such as windows.
Ambient room lighting can enter the imaging path and affect the image quality.
Place the JuLI™ Stage on a level surface away from vibrations from other
pieces of equipment.
Operating temperature range: 5 - 40°C (41 - 104°F)
Relative humidity range: 20 - 95 %.
4.4 Cautions
IMPORTANT!
First use: Before operating the JuLI™ Stagesystem for the first time, we strongly
recommend allowing the instrument to equilibrate to room temperature for
20 minutes outside the incubator while the device is turned on.
If the JuLI™ Stage is to be operated inside an incubator, an additional warm-up
time of 2 hours is required to equilibrate it to 37 °C. During this time the JuLI™
Stage must be switched on.
In an incubator, the JuLI™ Stage and PC must be left turned on at all times.

Installation
18 JuLITM Stage - User Manual
The JuLI™ Stage is optimized for a standard size cell culture incubator.
It is recommended that the temperature of the JuLI™ Stage instrument can
equilibrate to the temperature of the incubator.
Environmental requirements
When you use the JuLI™ Stage inside an incubator, make sure that the temperature
of the laboratory never exceeds 15℃to 25℃. Temperature outside this range may
affect the performance of the JuLI™ Stage.
The well plate’s cover should be wetted using the cell culture media before
monitoring.
This step is recommended to avoid condensation inside the well plate cover for
monitoring.
☞Note: This procedure can increase the risk for contamination. When properly
warmed up some vessels do not show condensation and can be imaged without
pre-wetting the lid. This should especially be considered for critical samples.
Avoid exposing the JuLI™ Stageto UV light.
UV light may degrade components, including plastics. Damage from UV exposure
is not covered under the manufacturer’s warranty.
Always wipe the surfaces with ethanol-soaked paper towels.
Do not directly spray ethanol anywhere on the JuLI™ Stage.
☞Note: The objectives are especially prone to damage by excessive ethanol.
Please always clean the objectives with ethanol-wetted objective cleaning paper.

Installation
JuLI™ Stage - User Manual 19
Internet Connection and Windows Updates on Control PC
If the control PC is connected to the internet, the Windows®operating system is
automatically updated (and rebooted if necessary). In order to avoid errors during
operation, we recommend:
Check for Windows Updates regularly and make sure that all available security
updates have been downloaded and installed before starting a measurement.
Disable internet access temporarily during screening campaigns to avoid
unattended installation of updates or reboots during a screening run.

User interface
20 JuLITM Stage - User Manual
5 User interface
Preview menu
ThePreview menucontains all functions to quicklyset up thevessel layout, imaging
conditions, and acquire snapshots and short movies.
Setup menu
The Setup menu includes all functions of the Preview menu (except snapshot and
short movie functions) and additionally the experiment settings. For the definition of
the experiment you can set an experiment name, layout, manual focus (supported
by a focus info map), or autofocus, stitched or scratch mode, image format output,
and time lapse preferences. This is where you start your experiments.
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