new brunswick Innova 43 User manual

43/43R
Incubator Shakers
MANUAL NO: M1320-0050
Revision E
May 28, 2008
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 44 TALMADGE ROAD EDISON, NJ 08818-4005
Telephone: 1-732-287-1200 1-800-631-5417
Fax: 732-287-4222 Telex: 4753012 NBSCO
Internet: http://www.nbsc.com E-mail: bioinfo@nbsc.com

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Innova 43 & 43R Incubator Shakers M1320-0050 User’s Guide

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INTERNATIONAL OFFICES:
BELGIUM
New Brunswick Scientific NV-SA
Stationsstraat 180/4
3110 Rotselaar
België/Belgique
Tel: 32 (0)16 56 28 31
Fax: 32 (0)16 57 27 53
E-mail: sales@nbsnv-sa.be
GERMANY
New Brunswick Scientific GmbH
In Der Au 14
D-72622 Nürtingen
Deutschland
Tel: 49 (0)7022 932490
Fax: 49 (0)7022 32486
E-mail: sales@nbsgmbh.de
CHINA
New Brunswick Scientific Co., Inc.
A903, 904 Yin Hai Building
No. 250, Cao Xi Road
Shanghai 200235, P.R. China
Tel: 86 21 6484 5955 or 5966
Fax: 86 21 6484 5933
E-mail: nbschc@online.sh.cn
THE NETHERLANDS
New Brunswick Scientific BV
Kerkenbos 1101, 6546 BC Nijmegen
P.O Box 6826, 6503 GH Nijmegen
Nederland
Tel: 31 (0)24 3717 600
Fax: 31 (0)24 3717 640
E-mail: sales@nbsbv.nl
FRANCE
New Brunswick Scientific SARL
12-14, Rond Point des Champs Elysées
75008 Paris
France
Tel: 33 (0)1 5353 1511
Fax: 33 (0)1 5353 1557
E-mail: sales@nbssarl.fr
UNITED KINGDOM
New Brunswick Scientific (UK) Ltd.
17 Alban Park
St. Albans, Herts. AL4 0JJ
United Kingdom
Tel: 44 (0)1727 853855 or 0800 581331
Fax: 44 (0)1727 835666
E-mail: bioinfo@nbsuk.co.uk
Web: www.nbsuk.co.uk
New Brunswick Scientific Co., Inc. User’s Guide

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CAUTION!
This equipment must be operated as described in this manual.
If operational guidelines are not followed, equipment damage and
personal injury can occur.
Please read the entire User’s Guide before attempting to use this
unit. Do not use this equipment in a hazardous atmosphere or with
hazardous materials for which the equipment was not designed.
New Brunswick Scientific Co., Inc. (NBS) is not responsible for any
damage to this equipment that may result from the use of an
accessory not manufactured by NBS.
Innova 43 & 43R Incubator Shakers M1320-0050 User’s Guide

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Copyright Notice
New Brunswick Scientific Company, Inc.
Box 4005 44 Talmadge Road
Edison, New Jersey 08818-4005
Copyright 2007 New Brunswick Scientific Co., Inc.
All Rights Reserved.
Reproduction, adaptation, or translation without prior written permission from
New Brunswick Scientific is prohibited.
Disclaimer Notice
New Brunswick Scientific Co., Inc. reserves the right to change
information in this document without notice. Updates to information in this
document reflect our commitment to continuing product development and
improvement.
Manual Conventions
NOTE: Notes contain essential information that deserves
special attention.
CAUTION!
Caution messages appear before procedures which, if
caution is not observed, could result in damage to the
equipment.
WARNING!
Warning messages alert you to specific procedures or
practices which, if not followed correctly, could result in
serious personal injury.
Bold Text in boldface type emphasizes key words or
phrases.
This particular Warning message, whether found in the
manual or on the unit, means HOT SURFACE–and
therefore represents a potential danger to touch.
CRUSH WARNING!
Crush Warning messages alert you to specific
procedures or practices regarding heavy objects which,
if not followed correctly, could result in serious personal
injury .
Biohazard-related messages.
New Brunswick Scientific Co., Inc. User’s Guide

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Every Instrument manufactured by the
New Brunswick Scientific Co., Inc. is warranted
to be free from defects in material and workmanship.
This apparatus is warranted for three (3) years against faulty components
and assembly. During the first two (2) years of this warranty period,
New Brunswick Scientific Co., Inc. (NBS) will also cover labor charges
associated with the repair of this instrument. Our obligation under this
warranty is limited to repairing or replacing the instrument or part
thereof which shall, within three (3) years after the date of
shipment, prove to be defective after our examination.
This warranty does not extend to any NBS products that have been
subjected to misuse, neglect, accident or improper installation
or application; nor shall it extend to products that have
been repaired or altered outside the NBS
factory without prior authorization from
New Brunswick Scientific Co., Inc.
W A R R A N T Y
Innova 43 & 43R Incubator Shakers M1320-0050 User’s Guide

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TABLE OF CONTENTS
1OVERVIEW........................................................................................................................ 11
2INSPECTION & UNPACKING OF EQUIPMENT ....................................................... 13
2.1 INSPECTION OF BOXES ................................................................................................... 13
2.2 PACKING LIST VERIFICATION ........................................................................................ 13
2.3 UNPACKING OF EQUIPMENT ........................................................................................... 13
2.4 OUT OF BOX CONCERNS ................................................................................................ 13
2.5 WARRANTY REGISTRATION ........................................................................................... 13
3PREPARING THE LOCATION....................................................................................... 14
3.1 PHYSICAL LOCATION ..................................................................................................... 14
3.2 ENVIRONMENT............................................................................................................... 14
3.3 ELECTRICAL REQUIREMENTS......................................................................................... 14
3.4 SPACE REQUIREMENTS .................................................................................................. 15
3.5 NARROW DOORWAYS .................................................................................................... 15
4INSTALLATION................................................................................................................ 17
4.1 LEVELING THE SHAKER ................................................................................................. 17
5FEATURES......................................................................................................................... 19
5.1 CONTROLS ..................................................................................................................... 19
5.2 LCD DISPLAY ............................................................................................................... 20
5.3 CHANGING SCREENS ...................................................................................................... 20
5.4 DISPLAY ICONS .............................................................................................................. 21
5.5 ALARMS......................................................................................................................... 22
5.6 LID................................................................................................................................. 23
5.7 SPILL PAN/WATER RESERVOIR ...................................................................................... 23
5.8 SOFTWARE INTERFACES................................................................................................. 24
5.9 INTERIOR LIGHT(S) ........................................................................................................ 24
5.10 HEATER ......................................................................................................................... 24
5.11 REFRIGERATION (43R ONLY)......................................................................................... 24
5.12 SERVICE ACCESSIBILITY ................................................................................................ 25
6GETTING STARTED........................................................................................................ 26
6.1 PLATFORM ASSEMBLIES ................................................................................................ 26
6.2 INSTALLATION OF PLATFORM ........................................................................................ 26
6.3 INSTALLATION OF CLAMPS ............................................................................................ 26
6.4 ELECTRICAL CONNECTIONS ........................................................................................... 27
6.5 OPTIONAL GASSING MANIFOLD KIT .............................................................................. 28
6.6 OPTIONAL GERMICIDAL UV LAMP ................................................................................ 30
6.7 OPTIONAL PHOTOSYNTHETIC LAMPS ............................................................................. 30
6.8 OPTIONAL HUMIDITY MONITOR .................................................................................... 31
6.9 FILLING THE WATER RESERVOIR ................................................................................... 33
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6.10 DRAINING THE WATER RESERVOIR................................................................................ 34
7OPERATION ...................................................................................................................... 35
7.1 SAFETY PRECAUTIONS ................................................................................................... 35
7.2 OPENING THE LID .......................................................................................................... 35
7.3 STARTING THE SHAKER.................................................................................................. 36
7.4 USING THE LCD SCREENS ............................................................................................. 36
7.4.1 Display Screen ...................................................................................................... 36
7.4.2 Summary Screen.................................................................................................... 39
7.4.3 Setup Screen.......................................................................................................... 40
7.4.4 Lamps Screen........................................................................................................ 41
7.4.5 RS232 Screen ........................................................................................................ 43
7.4.6 Calibrate Screen ................................................................................................... 44
7.4.7 Programs Screen................................................................................................... 45
7.5 PROGRAMMING THE SHAKER ......................................................................................... 45
7.5.1 Timer Only ............................................................................................................ 45
7.5.2 Programmed Steps................................................................................................ 46
7.5.3 Creating a Program.............................................................................................. 46
7.5.4 Editing a Program ................................................................................................ 49
7.5.5 Running a Program............................................................................................... 50
7.6 MUTING THE AUDIBLE ALARM ...................................................................................... 50
7.7 TEMPERATURE OFFSET CALIBRATION ........................................................................... 50
7.7.1 Calculating the Offset Value................................................................................. 51
7.7.2 Setting the Offset................................................................................................... 51
7.8 USING CALSPEED ........................................................................................................... 52
7.9 POWER INTERRUPTION ................................................................................................... 52
8MAINTENANCE................................................................................................................ 54
8.1 ROUTINE MAINTENANCE ............................................................................................... 54
8.2 CLEANING EXTERNAL &INTERNAL SURFACES.............................................................. 54
8.3 BIOHAZARD DECONTAMINATION ................................................................................... 54
9SERVICE & ACCESSORIES ........................................................................................... 56
9.1 TROUBLESHOOTING ....................................................................................................... 56
9.2 PRODUCT RETURNS........................................................................................................ 58
9.3 OPENING THE SERVICE COMPARTMENT ......................................................................... 59
9.4 FUSE REPLACEMENT ...................................................................................................... 59
9.5 REPLACING CABINET LIGHTS......................................................................................... 60
9.6 REPLACING OPTIONAL PHOTOSYNTHETIC LAMPS.......................................................... 61
9.7 ADJUSTING LID TENSION ............................................................................................... 62
9.8 REPLACING OPTIONAL GERMICIDAL LAMP.................................................................... 63
9.9 BELT REPLACEMENT OR ADJUSTMENT .......................................................................... 64
9.10 REPLACEMENT PARTS.................................................................................................... 67
9.11 ACCESSORIES ................................................................................................................. 67
9.11.1 Platforms............................................................................................................... 67
9.11.2 Flask Clamps for Platforms.................................................................................. 68
Innova 43 & 43R Incubator Shakers M1320-0050 User’s Guide

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9.11.3 Replacement Clamp Hardware Kits ..................................................................... 69
9.11.4 Test Tube Racks & Other Accessories.................................................................. 69
9.11.5 Optional Gas Manifold Kit ................................................................................... 70
9.11.6 Optional Photosynthetic Lamps............................................................................ 70
9.11.7 Optional UV Germicidal Lamp............................................................................. 70
9.11.8 Optional Humidity Monitor .................................................................................. 70
9.11.9 Optional Utility Basket ......................................................................................... 70
9.11.10 Optional Remote Alarm .................................................................................... 71
10 DRAWINGS, GRAPHS & TABLES ............................................................................ 72
10.1 SCHEMATICS .................................................................................................................. 72
10.2 LOAD &SPEED GRAPHS................................................................................................. 73
10.3 LIST OF DRAWINGS ........................................................................................................ 75
10.4 LIST OF TABLES ............................................................................................................. 76
11 SPECIFICATIONS......................................................................................................... 77
11.1 CERTIFICATIONS ............................................................................................................ 78
11.1.1 CE Declaration of Conformity.............................................................................. 79
12 APPENDIX: REMOTE PROGRAMMING ............................................................... 80
12.1 SETTING UP WITH HYPERTERMINAL.............................................................................. 80
12.2 OVERVIEW OF COMMAND SETS ..................................................................................... 81
12.3 INDEX TO COMMAND CODES ......................................................................................... 82
12.4 SET COMMANDS ............................................................................................................ 83
12.5 PROFILE CONTROL COMMANDS ..................................................................................... 83
12.6 REPORT REQUEST COMMANDS ...................................................................................... 85
12.7 SET/GET DATE &TIME.................................................................................................. 85
13 APPENDIX: PRODUCT RETURNS........................................................................... 87
13.1 RETURN PROCEDURE ..................................................................................................... 87
13.2 RETURN AUTHORIZATION AND DECONTAMINATION CERTIFICATE ................................ 87
14 INDEX.............................................................................................................................. 89
New Brunswick Scientific Co., Inc. User’s Guide

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Innova 43 & 43R Incubator Shakers M1320-0050 User’s Guide

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1
1O
OV
VE
ER
RV
VI
IE
EW
W
The Innova 43/43R Incubator Shakers are large-capacity orbital shakers that utilize an eccentric
counterbalanced drive mechanism. They provide horizontal plane rotary motion in either a
1-inch (2.54 cm) or a 2-inch (5 cm) diameter circular orbit, depending on the model. A
Proportional/Integral (PI) microprocessor controller with instantaneous digital feedback controls
the speed over the entire speed range.
The Innova 43R provides temperature control from 20º below ambient (as low as 4ºC) to 80 C,
and the Innova 43 from 5º above ambient to 80ºC. Naturally, both these ranges depend on
relative humidity and other ambient factors, as well as the options installed in the unit. Ambient
temperature is measured at one meter from the exterior of the unit.
Erlenmeyer flasks (up to 6 liters in size) as well as a wide variety of tubes and plates can be
accommodated using the New Brunswick Scientific shaker accessories described in Section 9.11.
These are easily accessed on the platform.
To facilitate moving the unit, each shaker is equipped with casters that roll freely when the feet
are raised.
The Innova 43/43R may be operated in the following ways:
Continuously: at a set speed and temperature, until user intervention.
In a timed mode: run at a set speed, time and temperature for a period of up to 99.9
hours, after which the shaker automatically shuts off.
Via the shaker’s programmable controller: run through multiple temperature and
speed changes for an extended period of time.
Via computer through an RS-232 interface.
See Section 7 for more details on these various modes of operation.
For safe operation, the Innova 43/43R shakers are designed with a safety switch that
automatically stops the shaker mechanism when the lid is opened.
The Innova 43/43R is equipped with visual and/or audible alarms that alert the user to the
following conditions:
The end of a timed run
Deviations from speed setpoint
Deviations from temperature setpoint
Power failure
Lid open
New Brunswick Scientific Co., Inc. User’s Guide

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To accommodate customer needs, a wide variety of platforms can be used with the Innova
43/43R:
Universal platforms are the most flexible, providing hole patterns for flask clamps,
test tube racks and other accessories.
Dedicated platforms are supplied with flask clamps attached; they are designed solely
and expressly for this purpose.
Test tube racks, microplate holders, and test tube rack holders are also available (a
universal platform is needed for all test tube racks and holders).
For further information on these accessories, see Section 9.11.
We also recommend that you consult the load and speed graphs provided in Section 10.2.
Innova 43 & 43R Incubator Shakers M1320-0050 User’s Guide

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2
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NS
SP
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EC
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TI
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ON
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&U
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PA
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GO
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2.1 Inspection of Boxes
After you receive your order from New Brunswick Scientific, inspect the boxes carefully
for any damage that may have occurred during shipping. Report any damage
immediately to the carrier and to your local NBS Customer Service Department.
2.2 Packing List Verification
Verify against your NBS packing list that you have received all of the correct materials.
2.3 Unpacking of Equipment
CRUSH WARNING!
Do not attempt to lift the Innova 43/43R by hand.
Always use a lifter or other suitable equipment when raising or handling
the unit.
To unpack your Innova 43/43R, you will need the following tools:
Claw hammer
Scissors to cut nylon strapping
Tool to remove 3-inch (7.6 cm) metal staples
Save all packing materials and this user’s guide/operating manual.
2.4 Out of Box Concerns
If any part of your order was damaged during shipping, is missing pieces, or fails to
operate properly, please fill out the Customer Satisfaction Form 6300, included with your
equipment, and return it by fax.
2.5 Warranty Registration
Please complete and return your warranty card or register electronically at our Website:
www.nbsc.com
New Brunswick Scientific Co., Inc. User’s Guide

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3
3P
PR
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EP
PA
AR
RI
IN
NG
GT
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N
3.1 Physical Location
It is essential that the instrument be situated in an area where there is sufficient space for
the shaker to clear walls and potential obstructions during operation. The surface on
which the unit is placed must be smooth, level, and able to support the shaker under full
load operating conditions.
CRUSH WARNING!
Do not attempt to lift the Innova 43/43R by hand.
Always use a lifter or other suitable equipment when raising or
handling the unit.
NOTE:
The Innova 43/43R has casters and leveling feet. When you need to move
the unit across the floor, make sure the feet are raised enough so that the
casters can roll freely without scraping the floor with the feet. At all other
times, make sure the feet are down, to avoid any unintended rolling.
3.2 Environment
The shaker is designed to operate optimally in the following ambient conditions:
10º to 35°C
20 to 80% Relative Humidity (non-condensing)
3.3 Electrical Requirements
The Innova 43/43R can be equipped to run on:
100 Volts, 50 or 60 Hz, 1500 VA maximum
120 Volts, 60 Hz, 1500 VA maximum
230 Volts, 50 Hz, 1500 VA maximum
Check your shaker’s Electrical Specification Plate (located on the back of the unit) to be
sure the electrical requirements of your unit match the output of your electric supply. If
they do not match, contact your NBS representative.
Innova 43 & 43R Incubator Shakers M1320-0050 User’s Guide

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3.4 Space Requirements
Allow at least four inches (10 cm) around the shaker for proper ventilation and for access
to Power Switch and RS-232 port accessibility on the right side. Also allow enough
room above the shaker for the lid to be fully open (see Figure 1).
Be sure to keep the power plug and power outlet easily accessible to facilitate unplugging
the unit, as needed.
Figure 1: Innova 43/43R Front View
DEPTH:
32 inches (81 cm)
including front
panel protrusions WIDTH:
45.5 inches
(115.6 cm)
HEIGHT:
40.6 inches
(103 cm) with feet
NOTE:
Open lid adds
23.5 inches
(59.7 cm).
PEDAL TO
OPEN LID
HANDLE TO
OPEN LID
NOTE:
Be sure to allow at least four inches (10.5 cm) around shaker for
ventilation, access to power cord (rear panel), and access to power
switch and RS-232 port (right side).
3.5 Narrow Doorways
If you need to move the Innova 43/43R through a doorway that is less than 32.5 inches
(82.5 cm) wide, remove the front window cover to reduce the depth of the unit to 30.3
inches (77 cm):
1. Carefully remove the five screws on the underside of the plastic window cover,
retaining them for reuse.
New Brunswick Scientific Co., Inc. User’s Guide

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2. Being very careful not to hit the control knob or button, slide the window cover
upward to remove it from the spring clips on the front of the unit.
When the shaker is in the desired location, reinstall the window cover by reversing the
procedure.
Innova 43 & 43R Incubator Shakers M1320-0050 User’s Guide

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4
4I
IN
NS
ST
TA
AL
LL
LA
AT
TI
IO
ON
N
CRUSH WARNING!
Do not attempt to lift the Innova 43/43R by hand.
Always use a lifter or other suitable equipment when raising or handling
the unit.
4.1 Leveling the Shaker
NOTE:
The Innova 43/43R has casters and leveling feet. When you need to move
the unit across the floor, make sure the feet are raised enough so that the
casters can roll freely without scraping the floor with the feet. At all other
times, make sure the feet are down, to avoid any unintended rolling.
Make sure that the shaker is placed on a level surface, in its intended location.
Lower all four adjustable feet as described below until they are solidly on the surface. If
the shaker is not level, adjust the feet as needed to achieve leveling:
1. Immobilize the top lock nut against the unit with one wrench whenever you adjust
the foot, to keep the threaded stud from falling out (see Figure 2).
Figure 2: Adjustable Foot (as shipped)
BOTTOM
OF UNIT
LOCK NUT
FLATS FOR
WRENCH
FOOT
1.75 inch
4.45 cm
2. With a second wrench against the flats of the threaded stud, just above the foot.
Rotate clockwise to lower the foot or counter-clockwise to raise the foot.
New Brunswick Scientific Co., Inc. User’s Guide

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3. Place a level on the top of the unit. If necessary, make further adjustments by
repeating all steps until the unit is level.
4. After installing a platform (see Section 6), fully load the shaker and do a test run at
normal speed (see Section 7). Make additional leveling adjustments if necessary.
Innova 43 & 43R Incubator Shakers M1320-0050 User’s Guide

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5
5F
FE
EA
AT
TU
UR
RE
ES
S
5.1 Controls
Figure 3: Front Panel (detail)
POWER SWITCH
START/STOP
SWITCH
(for shaking)
DISPLAY
CONTROL
KNOB
RS-232 PORT
START/STOP SWITCH This switch is used to start or stop the shaker. It will also
activate the timer when a timed run is desired. If the unit
is stopped and restarted, the timer automatically returns
to the beginning of a run.
CONTROL KNOB This knob is multifunctional. It is used to change
screens, and to select and change operating conditions.
RS-232 PORT See Section 5.8 for details.
POWER SWITCH This rocker switch is a circuit breaker that turns power on
and off to the entire Innova 43/43R.
NOTE:
In addition to the power switch, the power cord is also used to conduct
power or to break the power circuit to the shaker. Whenever power to the
shaker may be a hazard (during cleaning, maintenance or service work),
be sure to disconnect the power cord from the electrical outlet.
New Brunswick Scientific Co., Inc. User’s Guide

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5.2 LCD Display
When the unit is powered up, using the Power Switch located on the right side of the
machine (see Figure 3), the initial display screen will remain for a short time while the
system boots up. Then the main screen, called DISP for Display, appears (see Figure 4).
This screen will indicate the same parameters that were in effect when the power was
turned off.
Figure 4: Display Screen
ºC 23
RPM 120
DISP We 13 : 44
PARAMETERS ACTUAL
PARAMETER
VALUES
SCREEN NAME
AUDIBLE ALARM
MUTED
LID OPEN
PARAMETERS
LOCKED
DAY &
24-HOUR TIME
PROGRAM RUNNING
HEATER ON
TEMPERATURE OFFSET
For an explanation of the icons on the display screen, see Section 5.4.
Turning the Control Knob will highlight functions and/or values that can be changed.
Whenever you turn the Control Knob, the chamber light will go on (for details, see
Section 5.9).
For more information on working in the Display Screen, see Section 7.4.1.
5.3 Changing Screens
You can change screens displayed by highlighting the screen name field in the lower left
corner, pressing the Control Knob in until it clicks, rotating the Knob left or right (which
also makes clicking sounds) to the desired screen and clicking the Control Knob in again.
Table 1 describes the various screens:
Innova 43 & 43R Incubator Shakers M1320-0050 User’s Guide
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