Nippon Genetics FastGene User manual

NIPPON Genecs EUROPE GmbH Mariaweilerstraße 28-30, 52349 Düren, Germany,
Tel: [phone], Fax: [phone], E-Mail: info@nippongenecs.de, www.nippongenecs.eu
FAS-BG LED BOX
GP-04LED Manual

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
2

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
3
Content
P. 5
P. 5
P. 5
P. 6
P. 6
P. 7
P. 8
P. 8
P. 9
P. 9
P. 9
P. 10
P. 11
P. 13
P. 14
P. 15
P. 16
P. 17
P. 18
1. GENERAL INFORMATION......................................................................................................
1.1 Warranty.................................................................................................................
 1.2Regulatorynoce..................................................................................................
 1.3Safetyinformaon................................................................................................
 1.4Contactinformaon............................................................................................
2. TECHNICAL SPECIFICATIONS...............................................................................................
3. SYSTEM OPERATION...............................................................................................................
 3.1Productcontentandoverview...........................................................................
 3.2Inialsetup.............................................................................................................
 3.3Agarosegelscanning(Blue/GreenLEDlight).................................................
 3.3.1Gelposioning...................................................................................................
 3.3.2Exposuremecorrecon.................................................................................
 3.3.3Imageviewermenuandexternalstoragetransfer....................................
 3.4DNAextraconfromagarosegels...................................................................
 3.5Membrane/Petridishscanning(Whiteepilight)...........................................
 3.6Colorimetricgelscanning(WhiteLEDlighttransilluminator)....................
 3.7.Generalsengs...................................................................................................
 3.8.Connecnganexternalprinter.........................................................................
 3.9.Connecnganexternal(touch)monitor.........................................................

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
4

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
5
1. GENERAL Informaon
Thankyouforpurchasingthe FastGene®FAS-BGLEDBOXImagingSystem,astand-alonegeldocumen-
taonsystem.TheBlue/GreenLEDtechnologypermitsthedeteconofDNAwithhighestsensivityand
withoutharmingyoureyes,skinoryoursample.WiththewhiteLEDlightyoucanimageproteingelsstained
withcoomassieorsilverstaining.ThewhiteLEDepi-illuminaonallowsthedocumentaonofopaquesur-
facessuchasPetridishesorWesternBlotmembranes.
PleasereadcarefullythefollowingnotestobeabletoproperlyusetheFastGene®FAS-BGLEDBOXImaging
System.
1.1 Warranty
TheFastGene®FAS-BGLEDBOXiswarrantedagainstdefectsinmaterialsandworkmanshipfor1year.In
caseofanydefectsoccurringintheinstrumentoraccessoriesduringthiswarrantyperiod,NIPPONGenecs
EUROPEwillrepairorreplacethedefecvepartsatitsdiscreonwithoutcharge.
The following defects, however, are specically excluded:
 -Defectscausedbyimproperoperaon.
 -RepairormodicaondonebyanyoneotherthanNIPPONGenecsEUROPE
  oranauthorizedagent.
 -Damagecausedbysubstungalternaveparts.
 -UseofngsorsparepartssuppliedbyanyoneotherthanNIPPONGenecsEUROPE.
 -Damagecausedbyaccidentormisuse.
 -Damagecausedbydisaster.
 -Corrosioncausedbyimpropersolventorsample.
Foranyinquiryorrequestforrepairservice,contactNIPPONGenecsEUROPEoryourlocaldistributor.
Pleasesendamessagecontaininginformaonaboutthemodelandserialnumberofyourinstrument.
1.2 Regulatory noce
IMPORTANT:
ThisNIPPONGenecsEUROPEinstrumentisdesignedandceredtomeetsafetystandardsandEMC
regulaons.Ceredproductsaresafetousewhenoperatedinaccordancewiththeinstruconmanual.
Thisinstrumentmustnotbemodiedoralteredinanyway.Alteraonofthisinstrumentwill:
Voidthemanufacturer’swarranty
VoidthesafetyandEMCcercaon
Createapotenalsafetyhazard
NIPPONGenecsEUROPEisnotresponsibleforanyinjuryordamagecausedbytheuseofthisinstrument
forpurposesotherthanthoseforwhichitisintended,orbymodicaonsoftheinstrumentnotperformed
byNIPPONGenecsEUROPEoranauthorizedagent.

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
6
1.3 Safety informaon
Pleasecarefullyreadthefollowingsafetyinformaonnotestobeabletoproperlyusethe
FastGene®FAS-BGLEDBOX:
Please wear appropriate personal protecve equipment. Please read the instrucons manual to properly
usetheFastGene®FAS-BGLEDBOX.UsinginstrumentswithFastGene®Blue/GreenLEDtechnologytrans-
illuminatorswillnormallynotdamagesamplesoreyesandskinoftheuser.However,prolongedexposure
ofhumannakedeyeswithlightirradiaoninthebluespectrummayincreasetheprobabilityofsuering
fromrenadiseases.Therefore,werecommendusingtheamberltershieldincludedinthepackage,when
handlingtheBlue/GreenLEDtransilluminatorwhiletheFastGene®FAS-BGLEDBOXisopenandtheBlue/
GreenLEDlightisturnedon.
OperatetheFAS-BGLEDBOXonlyifitisconnectedtoasafetysocket.
Placetheunitonalevelsurfacewithminimalchanceofdropping.
1.4 Contact informaon
NIPPONGenecsEUROPEGmbH
Mariaweilerstraße28-30
52349Düren,Germany
Tel: [phone]
Fax: [phone]
E-Mail:info@nippongenecs.de
Formoredetailedproductinformaon,contactdetails,quesons,ortroubleshoongplease
visitourwebsitewww.nippongenecs.euorcontactusviainfo@nippongenecs.de.
FastGene®isaregisteredtrademarkofNIPPONGenecsEUROPE GmbH.

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
7
2. Technical Specicaons
Lightsources Blue/GreenLEDtransilluminator
Whiteepilight
WhiteLEDlighttransilluminator
Transilluminatedarea 16x11,5cm
Light sources
General Info
Power 12V,4.16A
Dimensions(HxDxW) 23x25.4x20.7cm
GelTools Amberltershield
Weight 3.2kg
Catalog number
GP-04LED FAS-BGLEDBOX
Image capture
Sensor CMOSSensor
Camera 8MPixelcamera
Image format TIFF,JPEGandPNG
Exposureme 0.2-2sec,21exposuresteps
Display/Soware/Connecons
Display 5"ColorLCDTouchPanel
Soware Inbuiltcontrolsoware
Connecons 2xUSBPort(1xfront,1xback)
1xHDMIPort
Thermalprintersupport

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
8
DC power jack HDMI port
USB 2.0 port
GP-04LED Product Content
FAS-BGLEDBOX
Usermanual
A/CPowerTransformer(12V,4.16A,DC)
Amberltershield
Blackvelvetsheet
3.1 Product content and overview
3. System Operaon
Upper lid
Power buon
5" LCD Touch Screen
and control menu
USB 2.0 port
Light source switch
Amber lter shield
Black velvet sheet

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
9
3.2 Inial setup
Removealltapesthatholditemsinplaceforshipping.PlacetheFastGene®FAS-BGLEDBOXunitonaat
surface.Connecttheincluded DCadaptertothe backoftheunit thenplug intopoweroutlet. Pushthe
powerbuon.
Iniate the desired applicaon
Agarosegelscanning(Blue/GreenLEDlight)(secon3.3)
DNAextraconfromgel(secon3.4)
Membrane/Petridishscanning(Whiteepilight)(secon3.5)
Colorimetricgelscanning(WhiteLEDlight)(secon3.6)
3.3 Agarose gel scanning (Blue/Green LED light)
3.3.1 Gel posioning
OpenthelidoftheFastGene®FAS-BGLEDBOX.Slidetheblackvelvetsheetintothegrooveofthelidto
coverthewhitelightareaofthelid(seegurebelow).Theblackvelvetsheetreducesthebackground.
Placethegeltraywiththeagarosegelontheviewareaandclosethelid.Note:theviewareaiswater-
proof.Hence,theusageofthegeltrayisnotobligatory.
Upper lid Insert black velvet sheet

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
10
Slidethelightsourceswitchonthefrontsideoftheinstrumenttothecyanlightsource(seegurebelow).
Press„B/G“(Blue/GreenLEDlight)onthecontrolmenu,displayedontherightsideofthescreen.
ThiswillturnontheBlue/GreenLEDlight.Aliveimageofthegelwillbedisplayedonthescreen.
3.3.2 Exposure me correcon
Usetheupanddownbuonsonthecontrolmenutochangeandcorrecttheexposuremeunltheappro-
priateexposureofthegelisreached.Theexposuremecanbeadjustedfrom-10(shortexposureme)to
+10(longexposureme).Themiddlebuondisplaysthecurrentexposurelevel(e.g.-2)
Capture
>
<
-2
Exposure
B/G WL
epi WL back
Connuous
Capture
Seng Image
Viewer

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
11
Press"Capture"tosavetheimagetotheinternalstorageoftheFAS-BGLED
BOX.Theimagecanlaterbetransferredonanexternalstoragedevice(USB-
drive).
If"CounnuousCapture"isselected,theFAS-BGLEDBOXwillautomacally
recordveimageswithanexposurerangingfrom-2to+2.Allveimageswill
besavedontheinternalstorageofthesystemandcanlaterbetransferredon
anexternalstoragedevice(USB-drive).
Capture
Connuous
Capture
3.3.3 Image viewer menu and external storage transfer
Pressthe"ImageViewer"buontoentertheimageviewermenuforviewing,
prinngandtransferringrecordedimages.
Image
Viewer
Thetopoftheimageviewermenudisplaysthename,exposurelevel,dateand
meofeachcurrentlydisplayedimage.Scrollthroughtherecordedimagesby
pressingtheleandrightbuons.
Rename
Return
Zoom In Zoom Out
Delete DeleteAll
Negative Print
Save Saveall
< >
20220202001
EV: -2
2022/02/02
10:23:43
Imagename,exposurelevel,
dateandme
Scrollthrough
recordedimages

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
12
InsertanexternalstoragedeviceinoneofthetwoUSB-ports(frontorback)of
theFAS-BGLED BOX.Press"Save"to transferthecurrentlydisplayedimage
totheexternalstoragedevice.Press"Saveall"totransferallimagescurrently
storedintheinternalstorage.
Tochangethenameoftheimagepress"Rename".Theimagenamecancontain
amaximumof15characters.
Save Saveall
Rename
Press"Negave"todisplaythecurrentimageinnegavecolours.Press"Save"
totransferthenegaveimageversiontotheexternalstoragedevice. Negative Save
IfanexternalprinterisconnectedtotheFAS-BGLEDBOXviaUSB(seesecon
3.8),thecurrentlydisplayedimagecanbedirectlyprintedbypressing"Print". Print
Press"Delete"todeletethecurrentlydisplayedimage.Press"DeleteAll"
todeleteallimagescurrentlystoredintheinternalstorage.
Usethe„Zoomin“and„Zoomout“buonstoenlargeaspecicareaofthegel.
Pleasenotethatthezoomfunconcanonlybeusedforviewingpurposesand
anenlargedimageviewcannotbesavedortransferred.
Delete DeleteAll
Zoom In Zoom Out
Press"Return"toreturntothepreviousdisplaymenu. Return

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
13
3.4 DNA extracon from agarose gels
TheFastGene®FAS-BGLEDBOXisequippedwithanamberltershieldtolterouttheBlue/GreenLED
excitaonlight.ThelterenablesvisualizaonofDNAbandsfortheextraconofDNAfromtheagarosegel.
Slidethelightsourceswitchonthefrontsideoftheinstrumenttothecyanlightsource.
OpentheupperlidoftheFAS-BGLEDBOX.TheBlue/GreenLEDlightwillautomacallyswitcho.
Posionthecentralopeningoftheamberltershieldinthehangingtab.Makesurethetwobentareasare
placedagainstthelid.
TurntheBlue/GreenLEDlightbackonbypressingthe"B/G"buon. B/G WL
epi
WL
back
ExcisevisibleDNAfragmentsfromthegelbylookingthroughtheamberltershield.
Upper lid
Amber lter shield

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
14
3.5 Membrane/Petri dish scanning (White epi light)
OpenthelidoftheFastGene®FAS-BGLEDBOX.Slidetheblackvelvetsheetintothegrooveofthelidto
coverthewhitelightareaofthelid(seegurebelow).Theblackvelvetsheetreducesthebackground.
Placethemembrane/Petridishontheviewareaandclosethelid.Slidethelightsourceswitchonthefront
sideoftheinstrumenttothewhitelightsource.
Press"WLepi"onthecontrolmenu,displayedontherightsideofthescreen.
Thiswillturnonthewhiteepilight.
B/G WL
epi
WL
back
Aliveimageofthemembrane/Petridishwillbedisplayedonthescreen.Refertosecon3.3.2forexpo-
suremecorreconandtosecon3.3.3forimageviewingandexternalstoragetransfer.
Upper lid Insert black velvet sheet

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
15
3.6 Colorimetric gel scanning (White LED light)
OpenthelidoftheFastGene®FAS-BGLEDBOX.Removetheblackvelvetsheetfromtheinsideofthelid.
Placethetransparentcolorimetric(protein)gelontheviewareaandclosethe
lid.Note:theviewareaiswater-proof.Slidethelightlourceswitchonthefront
side of the instrument to the white light source (see secon 3.5). Press "WL
back"onthecontrolmenu,displayedontherightsideofthescreen.Thiswill
turnonthewhiteLEDlighttransilluminator.
B/G WL
epi
WL
back
Aliveimageofthecolorimetricgelwillbedisplayedonthescreen.Refertosecon3.3.2forexposureme
correconandtosecon3.3.3forimageviewingandexternalstoragetransfer.
Upper lid Remove black velvet sheet

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
16
3.7 General sengs
Press"Seng"togettothegeneralsengsmenu. Seng
Thegeneralsengsmenu
displaysthefollowingscreen: UpdateS/W Sleep Date
Time Mouse
Save Format: JPEG
Color Type: Color
Thedevicesowarecanbeupdatedbypressing"UpdateS/W".Press"Sleep"toadjustthemeaerwhich
theFAS-BGLEDBOXgoesintosleepingmode.Youcanadjustthemebetween1min,5min,10min,30
minornever.Press"DateTime"tosetthedateandmeonthedevice.Itispossibletoconnectanexternal
mousetotheFAS-BGLEDBOXviaoneofthetwoUSB-ports.Acvatethe"Mouse"buontousethe
externalmouseanddeacvatethe"Mouse"buontousethedeviceviatouchscreen.
Mouse Mouse
Mouse acve Mouse inacve
Pressthebuonnextto"SaveFormat"tosettheimageletype.Thecurrentlyusedletypeisdisplayed
onthebuon.ImagelescanbesavedasTIF,JPEGorPNG.
TIF
JPEG
PNG
Pressthebuonnextto"ColorType"tosettheimagecolor.Thecurrentlyusedimagecolortypeisdisplay-
edonthebuon.TheimagecolorcanbesettoColororGray.

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
17
3.8 Connecng an external printer
ConnecttheexternalprintertotheFAS-BGLEDBOXviatheUSB-port.
PresstheImageviewerbuon.
Image
Viewer
Rename
Return
Zoom In Zoom Out
Delete DeleteAll
Negative Print
Save Saveall
< >
20220202001
EV: -2
2022/02/02
10:23:43
Press"Print"intheimageviewercontrolmenutodirectlyprintthedisplayedimageontheexternalprinter.

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
18
3.9 Connecng an external (touch) monitor
ConnecttheexternalmonitortotheHDMI-portoftheFAS-BGLEDBOX.Notethatthemonitorshould
beconnectedbeforeswitchingonthedevice.Theacvedisplayautomacallyswitchestotheexternal
monitor.ForusinganexternaltouchdisplayconnectitviatheHDMI-portandUSB-porttotheFAS-BG
LEDBOX.

NIPPON Genecs EUROPEFastGene® FAS-BG LED BOX
19

NIPPONGenecsEUROPEGmbH
[phone]
[phone]
info@nippongenecs.de
www.nippongenecs.eu