novel XSZ-107T Quick start guide
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Biological Microscope
Model XSZ-107T
With an Epi-Fluorescence (YX- ) attachment
USER AND OPERATIONAL
MANUAL
This manual is for the biological microscope XSZ-107T. To ensure the safety and optimum performance of this
unit, please ensure a thorough familiarization with this manual so as to become comfortable with the use of this
unit.
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Novel XSZ-107T with YX- Epi- Fluorescence Attachment
Contents
1. Application ................................................................................................................................................................ 3
2. Safety Note ............................................................................................................................................................ 3
3. Maintenance and care .......................................................................................................................................... 4
4. Components of the microscope ................................................................................................................................ 5
5. Assem ly ................................................................................................................................................................... 6
6. Assem ly Procedure ......................................................................................................................................... 7
6.1 Installing the epi-florescence unit ................................................................................................................. 7
6.2 Installing the inocular head ............................................................................................................................... 7
6.3 Installing the Eyepieces ................................................................................................................................. 8
6.4 Installing the o jectives ................................................................................................................................ 8
7. Connecting the power cords ................................................................................................................................. 9
8. Adjustment Set Diagram ..................................................................................................................................... 10
.................................................................................................................................................................................... 11
9. Adjustment and Operation for rightfield microscopy ...................................................................................... 12
9.1 Brightness Adjustment ................................................................................................................................ 12
10. Focusing on the specimen .............................................................................................................................. 13
10.1 Focus on the specimen ............................................................................................................................... 13
10.2 Condenser adjustment ................................................................................................................................ 13
10.4 Adjusting the interpupillary distance .......................................................................................................... 14
10.5 Adjusting the dioptre .................................................................................................................................. 14
10.6 Adjusting the tension adjustment collar ..................................................................................................... 14
11. Using the Epi-Florescence attachment for specimen o servation ................................................................. 14
11.1 Preparation ................................................................................................................................................. 15
13. Technical Specifications ...................................................................................................................................... 16
13.1 Main specifications of the microscope XSZ-107T ....................................................................................... 16
13.2 Main specifications of the epi-florescence attachment YX-2 ..................................................................... 16
12.1 Fluorescent Filter Com inations ................................................................................................................. 17
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1. Application
The XSZ-107 series microscope is a multi-purpose io-microscope that has een specifically designed for
clinical and chemical examination. The application and use of this series of microscopes is very diverse and
extends to professional research, teaching demonstration, use in medical and health esta lishments,
la oratories, technology, research and tertiary education facility. Its accessories make the instrument
capa le of accomplishing a very diverse and wide range of practical use. The XSZ-107 can e adapted in
many ways including phase contrast, florescence, photography, camera depiction, television and computer
ased image processing. The XSZ-107 can also e used for routine work in the fields of iology,
acteriology, histology, cytology, haematology and pharmacology easily (to name a few).
The microscope unit is used in conjunction with the epi-fluorescent attachment which has een designed
for use with and including our iological microscopes such as the Novel N-400M, Novel N320M and the
Novel XSZ-107CCD.
. Safety Note
a. Carefully open the ox, avoid the accessories dropping to the ground and eing damaged.
The epi-fluorescent attachment is a precision instrument.
. Please ensure that the entire unit is kept out of direct sunlight, high temperatures, humid or
dusty environments.
c. Make certain that the power cords are installed correctly and that all other components are
connected firmly.
d. Do not open any of the lamp housings while they are turned on or for at least 10 minutes
after they have een turned off. These parts are extremely hot and can cause urns if
touched.
e. Always e sure to ground (earth) the equipment.
f. Verify that the voltage and the frequency of the AC mains outlet match the setting of the
voltage switch and the frequency switch on the rear of the power supply units.
g. Always use the power cord provided y NOVEL and make sure that the main switch is
moved to “O” (OFF) efore connecting the power cord plug to the wall outlet.
h. To prevent any hazard, always turn the main switch on the power supply unit to “O” (OFF),
unplug the power cord plug from the mains outlet efore replacing any ul or the fuse, and
wait for at least 10 minutes efore replacing the ul .
i. To prevent o struction of the air flow, it is important to make sure to leave enough space
around and a ove the lamp housing of the microscope as well as epi-florescence
attachment.
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The following sym ols are found on the system. Study the meaning of the sym ols and always use
the equipment in the safest possi le manner.
Sym ol
Explanation
Indicates that the surface ecomes hot, and should not e touched with are hands。
Indicates that the high voltage (upper 1KV)
inside, improper
handing could result in an
electric shock to the use.
Before use, carefully read the user manual .Improper handing could r
esult in personal
injury to the user and/or damage to the equipment.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
● This manual is written for the XSZ-107T with an epi-fluorescent attachment YX-2 and efore
equipping it with any other ordinary microscope, e sure to learn how to use the microscope.
3. Maintenance and care
3.1 All the lenses have een adjusted properly, please do not dismount any lenses y oneself as this
will render any warranties / guarantees void.
3.2 The nosepiece and coarse / fine focus parts are very delicate and should under no
circumstances e dismantled y anyone who has not received correct training on how to repair
any faults that may occur.
3.3 Keep the units clean at all times. Do not place any hazardous chemicals near the units as the
lenses will get damaged and shall not e replaced under warranty.
3.4 If, under certain circumstances, certain contaminants end up on the lenses (i.e fingerprints, oil
smudges) one can clean these off with a soft cloth, tissue paper or gauze which has een slightly
immersed in pure alcohol or ether. Note that the alcohol or ether is extremely flammable;
please take extra precaution when using this method by keeping the cleaning solution and
cloth or material away from potential sources of electrical spark or fire.
3.5 Do not attempt to use any organic (live) solvents to clean the microscope or epi-florescence
unit and other glass components. To clean the microscope and exterior of the epi-florescence
unit, use of a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
3.6 While in use, if at any moment, some liquid of any form splashes onto the microscope,
immediately switch the unit off and remove any liquid that may still e visi le.
3.7 Do not dis-assem le any parts of the microscope or the epi-florescence unit as this will
compromise the function or reduce the overall performance of the unit.
3.8
Always place the unit in a cool, dry level surfaced location. When not in use, keep the unit
covered with the provided dust cover and make sure that the microscope as well as attached
epi-florescence unit has sufficiently cooled efore storing away
.
Safety Symbols
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4. Components of the microscope
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5. Assembly
Assembly Diagram
The Following figure shows the completed installation of the components: The corresponding figures show the
assem ly steps.
•Before installation, ensure the unit is clean as well as every component, do not store unpacked
units in a room which has high temperatures, high humidity or a dusty environment.
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6. Assembly Procedure
6.1Installing the epi-florescence unit
Place the epi-florescence unit on top of the microscope ody and tighten
the holding screw firmly to hold the unit in place
6.2
Installing the binocular head
Insert the inocular viewing head into the epi-florescence
unit and then mount it onto the microscope and ensure it is in
the correct position and olt it down to ensure no movement
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6.3 Installing the Eyepieces
Insert the eyepiece into the eyepiece tu e until they are level with the
eyepiece tu es as shown
6. Installing the objectives
•Adjust the coarse focus kno until the support device of the
mechanical stage reaches its lowest limit position.
•Screw the lowest magnification o jective into the nosepiece from
the left or the right side and then revolve the nosepiece clockwise
and place one o jective per open socket until all the o jectives are
installed.
Clean the objectives regularly as the lenses are susceptible to dust.
When operating the microscope, always use the 10x (yellow band)
objective to find the specimen first, then rotate the nosepiece to
magnify the specimen.
When rotating the nosepiece, always make sure one hears the
“click” sound to know the objective is in place and ready for
observation.
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7.
Connecting the power cords
•The ca les and power cords are vulnera le when ent or twisted;
never su ject the power cords to excessive force.
•Set the main switches to the off “0” position efore
connecting any of the power cords.
•Insert the power cord jack into the power of the microscope unit
Safely.
7.1 Charging of Battery and switching on
• The “I“ position power is ON. You can use the unit plugged in, and
the attery will charge while in use.
• The “II” position indicator renders the power as OFF. You cannot
use incident illumination as the LED will e off. If plugged in
the internal attery unit will automatically charge.
• “0” power is OFF. There will e no charging of the attery and no
illumination of the LED even if the unit is plugged in to the AC
mains power supply.
Once charging of the internal attery has commenced, a red power
indicator shall e o served. If the internal attery has reached its full
charge capacity, the red light will extinguish and a green light will
appear. Once in use, if one disconnects from the main AC power – the
internal attery will hold enough current to illuminate the LED for up
to 8 hours without requiring a re-charge.
If the battery is completely drained, the microscope can be
used, plugged into the wall outlet. It is used on the same
application as a laptop.
•Red Light - When charging the microscope, if the LED
power indicator light is red, the battery is not fully
charged.
•Green Light - When the LED power indicator light green,
the battery is fully charged.
•
If the unit has been stored for more than a week without any
AC power, it is advisable to charge the unit up over a
minimum of 18 hours before normal operation.
Insert the power cord into the epi- fluorescence power module safely
- Or -
Insert the power cord into the attery ackup epi-florescence power
module safely. If the attery ackup epi-florescence power module has
een stored for more than a week without any AC power, it is advisa le to
charge the unit up over a minimum of 18 hours efore normal operation.
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8.
Adjustment Set Diagram
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9. Adjustment and Operation for brightfield microscopy
9.1 Brightness Adjustment
9.1.1 Connect the power cord and set the
main power switch on to the (on) position “I” .
9.1.2 Turning the rightness adjustment
kno , increase the rightness of the illumination set to the
users’ satisfaction.
9.1.3
Ensure the epi-florescence
attachment is set to the “0” setting, or in the middle setting
9.1.4 Place the specimen slide on the stage
in the centre and use the stage swing arm to secure the
slide in place.
9.1.5 Turn the vertical and horizontal
adjustment kno s of the mechanical ruler to move the
specimen to the required position.
•Be careful when changing objectives to another
magnification. If you are finished working with a
magnification with a short working distance (i.e. 40x /
100x OIL) and want to change to another one, be aware
that you may touch the specimen.
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10. Focusing on the specimen
10.1 Focus on the specimen
Focus on the specimen using the 10x o jective. To avoid
touching the specimen during focusing, you should raise the
stage to its maximum height adjustment and o serve
whether it is close to the o jective at first, then using the
fine focus slowly lower the stage while o serving through
the eyepieces until a clear crisp image is seen.
10. Condenser adjustment
Turn the condenser focusing kno to move the condenser up or
down. Raise the condenser when using the high magnification
o jectives, and conversely lower it when using a lower
magnification o jective.
•The condenser and objective are coaxial. The
condenser has been adjusted and pre-set before leaving the
factory, and the maximum height has been pre-set, so the end-
user has no need to adjust it oneself.
10.3 Aperture Iris Diaphragm Adjustment
Turn the aperture iris diaphragm lever to adjust the aperture iris
diaphragm.
•The aperture iris diaphragm is designed for the
adjustment of the numerical aperture, not for
brightness.
•Generally, setting the aperture iris diaphragm to 70 % –
80 % of the numerical aperture (N.A.) of the objective
in use will provide an image with good contrast. If one
chooses to observe the image of the aperture iris
diaphragm, one can remove one eyepiece and look
down the eyepiece tube. One will observe a dark ring
encroaching from the sides of the tube at the end of the
viewable range.
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10.4 Adjusting the interpupillary distance
The interpupillary distance range of the microscope head is 55mm ~
75mm.
Push the eyepieces together and then while looking through the
eyepieces, move the distance apart until the left and right fields of view
coincide correctly.
10.5 Adjusting the dioptre
Turn the eyepiece to adjust the dioptre while looking through it.
•
The diopter range of the eyepiece is approximately 5 diopter.
The number aligned to the line on the viewing head is the dioptre in
use.
10.6 Adjusting the tension adjustment collar
Turn the tension adjustment collar with your fingers. When the collar is
turned in the direction of the arrow in the figure displayed tension of the
coarse adjustment kno increases. Turning the collar in the opposite
direction as indicated decreases the tension.
If the stage descends on its own weight of the specimen goes out of
focus for no reason even when rought into focus using the fine
focus adjustment kno – it means that the tension collar needs to
e adjustment with more tension. Turn the tension ring in the
direction indicated and the specimen shall remain in focus.
11.
Using the Epi-Florescence attachment for specimen observation
B
-
excitation
filter
G
-
excitation
filter
G
-
cutoff
filter
B
-
cutoff
filter
Protection
f
ilter
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11.1 Preparation
11.1.1 Verify that the plug from the AC mains outlet is connected firmly into the connector of the
power supply units.
11.1.2 Make sure the power cord is connected firmly.
11.1.3 When transmitted light o servation is required, pull out the filter system and make the hole
in the light path.
11.1.4 Be sure to use immersion oil when using fluorescent OIL o jectives.
11.1.5 When it is required to interrupt o servation for a short period, use the shield in the assistant
excitation filter part.
11.1.6 Precautions on the specimen colour fading:
11.1.6.1 The system employs high-intensity excitation light to ena le ight
o servation of dark fluorescent specimens. As a result, if high-power o jectives are
used frequently colour fading of the specimen occurs early, degrading the view
(contrast) of fluorescent images. So it is effective to use the shutter frequently to
avoid illuminating the specimen for a longer period than required.
11.1.6.2 Colour fading of the specimen can also e delayed using commercially
availa le colour fading preventing agent (DABCO, etc). The use of colour fading
preventing agent is recommended when you perform high-magnification
o servation frequently.
★ Note that colour fading prevention agent cannot e used with certain specimens.
1 .
Switching on Electrical Source of the LED driven epi-florescence unit
•
Set the main switch of the power supply unit to “1” (ON). The LED diode will e at full
capacity immediately after ignition.
•
To avoid shortening the urner life, do not turn the urner off and on repeatedly
•
The LED Diode is at full temperature and efficiency after 30 seconds and the aluminium
holder can warm considera ly after a out 10 minutes, it is advisa le to leave the unit off for
approximately 10 minutes efore moving the unit to allow sufficient cooling to occur.
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13.
Technical Specifications
13
.
1
Main specifications of the microscope XSZ-107T
Optical System
160mm path length
Viewing head
Siedentopf Binocular Head, inclined at 30° I
nterpupillary distance of
55 – 75mm
Eyepiece
Wide Field Eyepiece
WF 10x / 18
Magnification 10x
Focal length 25mm
Field of View 18mm
Nosepiece
Quadruple nosepiece
Focusing
Coaxial Coarse and Fine Focus adjustment, moving range 20mm, Fine Focus
Division 0.002mm
Condenser
ABBE condenser, NA = 1.25 with iris diaphragm
Stage
Dou le layer mechanical stage 140mm x 140mm.
Moving range of 75mm x 50mm
Illumination
3.6V 1w Ultra
-
Bright 350mA LED illumination
Power Supply
AC Input 200V or 110V
DC power output 5.5V
Battery assistance for power outages 3.6V 3800mAh (8 Hours operation)
13. Main specifications of the epi-florescence attachment YX-
Epi
-
Fluorescent
Illumination
Fluorescent Filter Specifications
B Exciting Light Filter System
G Exciting Light Filter
System
Illumination
3w Ultra Bright CREE
-
XML LED Diode
Protection Barrier
Barrier to Resist the Ultraviolet Light
Power Supply
Power supplier AC Input 220V/110V
(Interchangea le) 3.6v 1000mA constant current DC output
Power supplier AC Input 220V/110V
(Interchangea le) 3.6v 1000mA constant current DC output with attery
ack-up 10000 mAh
O jective
160mm
Fluorescence Free O jective
10
X
160mm
Fluorescence Free O jective 20
X
160mm
Fluorescence Free O jective 40X
160mm
Fluorescence Free O jective 100X
OIL
Immersion Oil
Fluorescence Free Oil
Vertical Illumination
●
Achromatic Optics System
● Filtering System
●
O servation Methods
•
Fluorescence
•
Transmitted Light (Brightfield)
Lamp Housing
●
Aluminium Lamp Housing
● CREE XM-L 3w LED Illuminator
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Operating
Environment
●Indoor Use
●Altitude: Max. 2000m
●Am ient Temperature: 5℃ to 40℃ (41℉ to 104℉)
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12.1
Fluorescent Filter Combinations
excitation
applications
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