Ryf Axio Lab.A1 User manual

Operating Manual Axio Lab.A1
Ryf AG Bettlachstrasse 2 2540 Grenchen tel [phone] fax [phone]
www.ryfag.ch

Carl Zeiss Copyright / Trade Marks Axio Lab.A1
2 430037-7144-001 04/2013
Knowledge of this manual is essential for the operation of the instrument. Please familiarize yourself with
the contents of this manual and pay special attention to instructions concerning safe operation of the
instrument.
The specifications are subject to change; the manual is not covered by an update service.
© Unless expressly authorized, dissemination or duplication of this document and commercial
exploitation or communication of its contents is not permitted. Persons in contravention of this
copyright are liable to pay compensation for damages.
All rights reserved, including rights created by patent grant or registration of a utility model.
All names of companies and products mentioned in this manual may be trademarks or registered
trademarks. Third party products are cited for information purposes only and this does not represent
approval or recommendation of these products.
Carl Zeiss Microscopy GmbH accepts no liability for the performance or use of such products.
Publisher: Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
[email protected]
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Königsallee 9-21
37081 Göttingen, Germany
Order No. 430037-7144-001
Date of issue: Version 1 - 19 April 2013

Axio Lab.A1 Contents / List of Illustrations Carl Zeiss
04/2013 430037-7144-001 3
CONTENTS
Page
1INTRODUCTION ...................................................................................................7
1.1 Notes on instrument safety ..........................................................................................7
1.2 Notes on ergonomics of the microscope....................................................................12
1.3 Notes on warranty ......................................................................................................13
2DESCRIPTION OF THE INSTRUMENT.................................................................14
2.1 Intended use................................................................................................................14
2.2 System overview.........................................................................................................16
2.3 Technical data..............................................................................................................21
2.3.1 Viewing height and tube angle.......................................................................................23
2.3.2 Assignment of dust covers, intermediate plate and base plate .........................................24
2.4 Control and functional elements on microscope.......................................................26
2.4.1 Stand models.................................................................................................................26
2.4.2 Stand for transmitted light..............................................................................................26
2.4.3 Stand for transmitted light polarization...........................................................................28
2.4.4 Stand for transmitted light and reflected light fluorescence .............................................30
2.4.5 Stand for reflected light..................................................................................................32
2.4.6 Stand for transmitted light conoscopy.............................................................................34
2.4.7 Ergo stands with TÜV certificate "Ergonomically tested" .................................................36
2.5 Control and functional elements on optional components ......................................37
2.5.1 Tubes/photo tubes .........................................................................................................37
2.5.2 Microscope stages..........................................................................................................41
2.5.3 Condensers....................................................................................................................43
2.5.4 Reflector turret 4x ..........................................................................................................45
3START-UP...........................................................................................................46
3.1 Installing standard components .................................................................................46
3.1.1 Unpacking and setting up microscope stand ...................................................................46
3.1.2 Mounting base plate for use of larger tubes....................................................................47
3.1.3 Attaching the binocular tube/photo tube ........................................................................48
3.1.4 Installing eyepieces or auxiliary microscope or diopter......................................................49
3.1.5 Screwing in objectives ....................................................................................................50
3.1.6 Fitting and removing push&click modules in the reflector turret.......................................51
3.1.7 Mounting a mechanical stage.........................................................................................52
3.1.8 Mounting rotary stage Pol ..............................................................................................54
3.1.9 Attaching condenser ......................................................................................................57
3.1.10 Installing or replacing 35 W halogen bulb or 3 W white light LED lamp............................58
3.1.11 Installing or changing the 12 V 50 W halogen lamp ........................................................60
3.1.12 Installing or replacing LED modules.................................................................................61

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3.2 Mounting optional components ................................................................................ 62
3.2.1 Mounting the light intensive co-observer unit ................................................................. 62
3.2.2 Mounting polarizer D or filter holder .............................................................................. 62
3.2.3 Mounting and centering the overview fixture.................................................................. 63
3.2.4 Inserting modulator disk in condenser 0.9 H Pol.............................................................. 64
3.3 Connecting to the power supply................................................................................ 65
3.4 Switching the microscope on/off............................................................................... 65
3.5 Basic setting of the microscope from an ergonomic point of view.......................... 66
3.5.1 Configuration of an ergonomic microscope workplace.................................................... 66
3.5.2 TÜV certificate ID:0000025994 "Ergonomically tested" .................................................. 67
3.5.3 Ergonomic configuration of the microscope workstation................................................. 67
3.5.4 Ergonomic adjustment of the microscope ....................................................................... 68
3.5.5 Setting interpupillary distance on the binocular tube....................................................... 70
3.5.6 Setting the viewing height ............................................................................................. 70
3.5.7 Adjusting for ametropia when using eyepiece reticles ..................................................... 71
4OPERATION ....................................................................................................... 72
4.1 Lighting and contrasting method in transmitted light ............................................. 72
4.1.1 Adjusting the transmitted light brightfield according to KÖHLER ..................................... 72
4.1.2 Adjusting the transmitted light darkfield according to KÖHLER........................................ 75
4.1.3 Setting the transmitted light phase contrast.................................................................... 76
4.1.4 Setting transmitted light polarization.............................................................................. 78
4.1.5 Setting transmitted light polarization with the conoscopy stand ...................................... 87
4.1.6 Determining the optical character of crystals................................................................... 87
4.1.7 Setting transmitted light polarization for conoscopic observation – determining the optical
character of crystals ....................................................................................................... 98
4.2 Lighting and contrasting method in reflected light................................................ 101
4.2.1 Adjusting the reflected light brightfield according to KÖHLER........................................ 101
4.2.2 Adjusting the reflected light darkfield........................................................................... 103
4.2.3 Adjusting reflected light polarization – Proof of bireflexion and reflexion-pleochroism.... 104
4.2.4 Adjusting reflected light fluorescence ........................................................................... 105
5CARE, FUSE REPLACEMENT AND SERVICE..................................................... 107
5.1 Instrument care......................................................................................................... 107
5.2 Instrument maintenance .......................................................................................... 108
5.2.1 Checking the instrument.............................................................................................. 108
5.2.2 Replacing the fuses in the stand ................................................................................... 108
5.3 Troubleshooting........................................................................................................ 109
5.4 Service ....................................................................................................................... 112
6ANNEX............................................................................................................. 113
6.1 List of abbreviations ................................................................................................. 113
6.2 Index.......................................................................................................................... 114
6.3 Industrial property rights ......................................................................................... 118

Axio Lab.A1 Contents / List of Illustrations Carl Zeiss
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LIST OF ILLUSTRATIONS
Fig. 1-1 "RADIATION" and "LED APERTURE" warning labels on Axio Lab.A1 for transmitted
and reflected light fluorescence ........................................................................................ 10
Fig. 1-2 "Hot surface below" warning label on Axio Lab.A1 for reflected light................................ 11
Fig. 2-1 Axio Lab.A1, stand transmitted light ................................................................................. 27
Fig. 2-2 Axio Lab.A1, stand transmitted light polarization............................................................... 29
Fig. 2-3 Axio Lab.A1, stand transmitted and reflected light fluorescence......................................... 31
Fig. 2-4 Axio Lab.A1, stand reflected light...................................................................................... 33
Fig. 2-5 Axio Lab.A1, stand transmitted light conoscopy................................................................. 35
Fig. 2-6 Axio Lab.A1 ergonomic stand with TÜV certificate "Ergonomically tested"......................... 36
Fig. 2-7 Binocular photo tube 30°/20 with fixed graduation 50:50.................................................. 37
Fig. 2-8 Binocular photo tube 30°/23 with toggle graduation 100:0/0:100 ..................................... 37
Fig. 2-9 Setting the viewing height on the binocular tube............................................................... 38
Fig. 2-10 Binocular ergo photo tube 8-38°/20 with fixed graduation 50:50....................................... 38
Fig. 2-11 Binocular ergo tube 8-33°/20 with vertical adjustment 50 mm........................................... 39
Fig. 2-12 Binocular ergo photo tube 20°/23 with vertical adjustment................................................ 40
Fig. 2-13 Binocular ergo photo tube 15°/23, telescopic with vertical adjustment ............................... 40
Fig. 2-14 Mechanical stage 75x30 R with specimen holder............................................................... 41
Fig. 2-15 Mechanical stage 75x30 R ergonomic with stationary drive................................................ 41
Fig. 2-16 Mechanical stage reflected light 75x30 R with specimen holding plate............................... 41
Fig. 2-17 Rotary stage Pol
................................................................................................................ 42
Fig. 2-18 Filter mount on luminous-field diaphragm operating ring for filter d=32x4 mm .................. 42
Fig. 2-19 Condenser 0.9/1.25 H, D, Ph1, Ph2, Ph3 with modulator disk............................................ 43
Fig. 2-20 Condenser 0.9/1.25 H ...................................................................................................... 43
Fig. 2-21 Overview fixture ............................................................................................................... 44
Fig. 2-22 Polarizers.......................................................................................................................... 44
Fig. 2-23 4-position reflector turret.................................................................................................. 45
Fig. 2-24 Nosepiece of the transmitted light polarization stand with mount for compensators........... 45
Fig. 3-1 Setting up the microscope ................................................................................................ 46
Fig. 3-2 Placing tools in the storage compartment.......................................................................... 46
Fig. 3-3 Stowing power cord in cover flap for shipping................................................................... 47
Fig. 3-4 Mounting the base plate................................................................................................... 47
Fig. 3-5 Attaching the binocular tube ............................................................................................ 48
Fig. 3-6 Installing the eyepieces ..................................................................................................... 49
Fig. 3-7 Installing the eyepiece reticle............................................................................................. 49
Fig. 3-8 Screwing in objectives....................................................................................................... 50
Fig. 3-9 Replacing the reflector module ......................................................................................... 51
Fig. 3-10 Replacing a mechanical stage............................................................................................ 52
Fig. 3-11 Setting friction torque....................................................................................................... 53
Fig. 3-12 Replacing snap-in rotary stage Pol, detachable specimen guide Pol and stage clips ............. 54
Fig. 3-13 Centring rotary stage Pol .................................................................................................. 55
Fig. 3-14 Centering objectives ......................................................................................................... 56
Fig. 3-15 Attaching condenser ........................................................................................................ 57
Fig. 3-16 Removing the cover.......................................................................................................... 58
Fig. 3-17 Removing the LED lamp.................................................................................................... 58

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Fig. 3-18 Inserting the LED lamp ......................................................................................................58
Fig. 3-19 Attaching condenser .........................................................................................................59
Fig. 3-20 Removing the cover...........................................................................................................60
Fig. 3-21 Removing the 12V 50W halogen bulb ...............................................................................60
Fig. 3-22 Installing the 12V 50W halogen bulb.................................................................................60
Fig. 3-23 Removing the cover...........................................................................................................61
Fig. 3-24 Removing the LED module.................................................................................................61
Fig. 3-25 Installing the LED module ..................................................................................................61
Fig. 3-26 Mounting the polarizer D ..................................................................................................62
Fig. 3-27 Mounting the overview fixture...........................................................................................63
Fig. 3-28 Modulator disk in condenser 0.9 H Pol...............................................................................64
Fig. 3-29 Mains power supply on rear side of stand ..........................................................................65
Fig. 3-30 Mains switch on left side of microscope.............................................................................65
Fig. 3-31 Light intensity control and FL/TL toggle switch....................................................................65
Fig. 3-32 TÜV certificate "Ergonomically tested" ..............................................................................67
Fig. 3-33 Ergonomic adjustment of the microscope ..........................................................................68
Fig. 3-34 Setting the interpupillary distance on the binocular tube ....................................................70
Fig. 3-35 Setting the viewing height on the binocular tube ...............................................................70
Fig. 4-1 Microscope settings in transmitted light brightfield ............................................................73
Fig. 4-2 Setting the height stop on the condenser carrier ................................................................74
Fig. 4-3 Center darkfield stop on condenser, achromatic-aplanatic 0.9 H D Ph DIC ..........................76
Fig. 4-4 Centering the phase stop (bright ring in condenser) to the phase ring
(dark ring in objective).......................................................................................................77
Fig. 4-5 Components for transmitted light polarization ...................................................................79
Fig. 4-6 Gamma direction ..............................................................................................................80
Fig. 4-7 Determining the direction of oscillation nJ’using a synthetic fiber as an example ................81
Fig. 4-8 Schematic diagram of the color charts according to Michel-Lévy
.........................................83
Fig. 4-9 Components for circular polarization contrast ....................................................................86
Fig. 4-10 Axio Lab.A1 for transmitted light conoscopy ......................................................................88
Fig. 4-11 Determining optical character............................................................................................89
Fig. 4-12 Components for transmitted light polarization on conoscopy stand ....................................91
Fig. 4-13 Gamma direction ..............................................................................................................91
Fig. 4-14 Determining the direction of oscillation nJ’using a synthetic fiber as an example.................92
Fig. 4-15 Schematic diagram of the color charts according to Michel-Lévy.........................................94
Fig. 4-16 Components for circular polarization contrast on conoscopy stand .....................................97
Fig. 4-17 Axio Lab.A1 for transmitted light conoscopy ......................................................................99
Fig. 4-18 Determining the optical character....................................................................................100
Fig. 4-19 Microscope settings in reflected light brightfield...............................................................102
Fig. 4-20 Components for reflected light fluorescence ....................................................................106
Fig. 5-1 Replacing the fuses in the stand ......................................................................................108

INTRODUCTION
Axio Lab.A1 Notes on instrument safety Carl Zeiss
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1INTRODUCTION
1.1 Notes on instrument safety
Axio Lab.A1 microscopes have been designed, produced and tested in compliance with DIN EN 61010-1
(IEC 61010-1) and IEC 61010-2-101 safety requirements for electrical measuring, control and laboratory
instruments.
These instruments meet the requirements of EC Directive IVDD 98/79/EC (In Vitro Diagnostic); they carry
the mark.
This operating manual includes information and warnings which must be observed by the user.
The following warning and instruction symbols are used in this manual:
CAUTION
This symbol indicates a potential hazard to the user.
CAUTION
Hot surface!
CAUTION
UV radiation emitted!
CAUTION
Disconnect the instrument from the power supply before opening!
CAUTION
This symbol indicates a potential hazard to the instrument or system.
NOTE
This symbol denotes a warning to which special attention must be paid.
Axio Lab.A1 microscopes, including original accessories, may only be used for the microscopy methods
described in this manual.
The following instructions must be carefully observed:
The manufacturer is unable to assume any liability for any other use, including individual
modules and individual components. This also applies to all service and repair work not
performed by authorized service personnel. Furthermore, all warranty rights will be forfeited.
The socket into which the power cable is plugged must be earthed. The grounding effect must
not be made ineffective by an extension cable that does not have a protective ground wire.

INTRODUCTION
Carl Zeiss Notes on instrument safety Axio Lab.A1
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Should it be determined that protective measures are no longer effective, the instrument must
be taken out of service and secured against unintended use. Please contact a Zeiss service
agency or the Carl Zeiss Microscopy Service to repair the instrument.
Axio Lab.A1 microscopes are equipped with a power supply unit incorporated in the stand,
which allows line voltages ranging from 100 V to 240 V ±10 % (50/60 Hz) to be used without
requiring any change of voltage on the unit.
Always pull the mains plug before opening the instrument or replacing a fuse!
Only fuses for the rated power may be used. The use of provisional fuses or short-circuiting the
fuse-holder is prohibited.
Axio Lab.A1 microscopes are not equipped with any special devices for protection from
substances that are corrosive, potentially infectious, toxic, radioactive, or other substances that
could be hazardous to health. Make sure to observe all legal regulations, particularly the
relevant national accident prevention regulations when handling such substances.
Dirt and dust may impair the performance of the instrument. Therefore, the instrument must be
protected from these influences as far as possible and covered with the dust cap when not in
use. Always check whether the instrument is switched off before you cover it.
Obstruction or covering of ventilation slits may result in heat build-up which may damage the
instrument and in extreme cases cause fire. Always keep the ventilation slits clear and ensure
that no objects enter the instrument through the ventilation slits.
Instruments may only be operated by trained personnel. The latter must be aware of the
possible hazards involved with microscopy and the respective application. Axio Lab.A1
microscopes are high-precision instruments whose functional capabilities may be impaired or
destroyed when handled improperly.
Instruments should not be operated in an explosive environment.
Instruments should only be operated on a solid, non-combustible surface.

INTRODUCTION
Axio Lab.A1 Notes on instrument safety Carl Zeiss
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LED Risk Group 2 to IEC 62471, LED radiation will be emitted.
Never look into the LED beam of the illuminating device – either with or without optical
instruments. Failure to observe this precaution may results in eye injuries!
Combustible and easily inflammable materials should not be held close to the light beam.
Be sure to read the safety data sheets for the Immersol 518 N£, Immersol 518 F£and
Immersol W£.
Immersol 518 N£immersion oil irritates the skin. Avoid any contact with skin, eyes and
clothing.
In the case of inadvertent skin contact, wash the oil off with plenty of water and soap.
In the case of inadvertent eye contact, immediately rinse the eye with plenty of water for at
least five minutes. Seek medical attention if irritation persists.
Proper disposal of immersion oil 518 N£: do not discharge into the surface water or sewerage
system.
Do not put defective microscopes into the household waste; they must be disposed of in
accordance with WEEE Directive 2002/96/EC.
Samples must likewise be properly disposed of in accordance with statutory provisions and
internal work instructions.

INTRODUCTION
Carl Zeiss Notes on instrument safety Axio Lab.A1
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Warning labels on Axio Lab.A1 stands
Warning label: Hot surface!
Affixed to all stands with transmitted light illumination.
Fig. 1-1 "RADIATION" and "LED APERTURE" warning labels on Axio Lab.A1 for transmitted and
reflected light fluorescence