NuAire NU-99338JE User manual

NuAire Ultra Low Temperature
Freezer User Manual
OM0278
OM0278
Revision 2 May/2021
1 of 32
Release date: 5/17/2021
Introduction
Models:
NU-99338JE
NU-99486JE
NU-99578JE
NU-99728JE
NU-99828JE
NU-99420JE
NU-99338JGA
NU-99578JGA
NU-99728JGA
NU-99338JG
NU-99578JG
NU-99728JG
NU-99828JG
NU-99420JG

NuAire Ultra Low Temperature
Freezer User Manual
OM0278
OM0278
Revision 2 May/2021
2 of 32
Release date: 5/17/2021
Contents:
Product Features
1.0 It merits your trust from beginning to end
2.0 Temperature Control
3.0 Security System
4.0 Refrigeration system
5.0 User friendly design
6.0 Safety Label and Safety Precautions
7.0 Usage Precautions
Installation
8.0 Installation environment
9.0 Installation Location
10.0 Before Use Preparation
11.0 Initially Powering up
12.0 Operation after a power Outage
13.0 Freezer Parts
13.1 Vertical Type
13.2 Horizontal Type
14.0 Control Panels
15.0 How to Use the Freezer
15.1 Unlocking the Freezer
15.2 Setting the inner temperature
15.3 Setting the high temperature alarm
15.4 Setting the security code
15.5 Setting the startup delay timer
15.6 Alarm Test and low battery Test
15.7 Setting the USB storage time
15.8 Exporting USB data
16.0 Display and Alarm
17.0 Cleaning and Maintenance
18.0 Recycling the Rechargeable Battery
19.0 Optional Accessories
20.0 Troubleshooting
21.0 Refrigeration and Wiring Diagram
21.1 Refrigeration Diagram
21.2 Wiring diagram
22.0 Specifications, Technical Data and Packing list
22.1 Specification
22.2 Technical Data
22.3 Packing List
Warning:
A review should be conducted to establish risk associated with electromagnetic
compatibly. In the documentation for the user, a statement shall be included
indicates potential difficulties in ensuring electromagnetic compatibility in other
environments due to conductive as well as radiated disturbances.

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Freezer User Manual
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Release date: 5/17/2021
1.0 It merits your trust from beginning to end.
This Product is suitable for the ultra-low temperature storage of products in
applications such as clinical pharmaceutical, scientific research, and epidemic
institutions. It also can be used in blood stations, hospitals, centers for disease
prevention and control, science and research institutions, electronic and chemical
laboratories, biomedical engineering institutions, and open sea fishery companies to
store red blood cells, white blood cells, viruses, germs, skin, bones, bacteria, sperm,
biological products, electronic components, and low temperature testing samples of
special products, etc.
2.0 Temperature Control
Temperature is controlled by computer and numerically displayed and regulated in
units of 1°C; temperature range: -40°C to -86°C.
3.0 Security System
•Various malfunction alarms (high temperature alarm, power failure alarm, probe
failure alarm, hot condenser alarm, high ambient temperature alarm, door open
alarm, low battery alarm).
•Two types of alarms (Buzzer sounding alarm, Flashing light alarm).
•Protective functions (password protection, power up delay protection).
•All independent components are safely grounded.
4.0 Refrigeration system
•Optimized multiple refrigeration technology with top brand compressors offering
better refrigeration capability.
•Excellent temperature stability.
•Exclusive sealing structure of multiple doors and hot tubing for condensation
prevention and front reduction.
•Specially designed low temperature computer control, to prevent the normal
redundant.
•System from being erroneously controlled by the low temperature compressor.
5.0 User friendly design
•Equipped with LED display which can show the inner temperature, set temperature,
ambient temperature and input voltage. The display can also be used to set the
high/low temperature alarm and inner temperature, as well as show any
malfunction alarms.
•Designed with adjustable shelves suitable for product storage.
•Safe lock design prevents accidental opening of doors.
•Broad ambient temperature range design, suitable for usage to 32°C environments.
•Innovative design with integrated door locks and compact leveling legs, which are
flexible and convenient.
•Automatically open and close condensation fan to save energy.
•Equipped with networks, remote alarm functions which are advanced and practical.
•USB standard.

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Freezer User Manual
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Release date: 5/17/2021
6.0 Safety Labels and Safety Precautions
Dear Customer
Please read this manual for safe operation and use of this product.

NuAire Ultra Low Temperature
Freezer User Manual
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Revision 2 May/2021
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Release date: 5/17/2021
When CO2/LN2 backup is activated, the location place must be well ventilated. Increased CO2
in the air may be harmful and even fatal. If the ventilation is poor, other methods should be
considered in order to ensure safe working environments.
If there is a leakage of petroleum gas or other flammable gas, close the gas supply
valve and open doors and windows to ventilate the air. Do not plug in or unplug your
freezer unit in order to avoid potential explosion or fire.
Only professional technicians or service personnel can install the unit.
Failure to do so may cause electric shock or result in a fire hazard.
The freezer must be securely installed on a firm floor. On an uneven surface may result
in the product tipping over thereby causing injury and damage.
Please use the dedicated power supply marked on the product label to avoid fire and
electric shock.
If the voltage being used is 10% higher than the rated voltage, a regulator with a
capacity of 4000 W or higher must be installed.
If the power cord needs to be extended, the cross-section of the extended cable must
be no less than 14Awg and no longer than 9ft for products of 220V~240V/50Hz or
220V~/60Hz and no less than 12Awg and no longer than 3m for products of
115V~/6Hz to avoid fire or electric shock.
Your ULT unit is equipped with a standard three-prong power plug (grounded)
complying with the standard three-prong socket (grounded) rated 16 a
(220V~240V/50Hz or 220V~/60Hz) or rated 20A (115V~/60Hz). Removal of the ground
prong be securely plugged into the socket.
A loose plug in the socket may cause fire.
The power socket intended for your ULT usage must be grounded to avoid electric
shock.
If the socket does not meet this requirement, the condition must be corrected by a
qualified technician before using the UL unit.
The replacement of any spare parts (battery, etc.) shall be conducted by approved
technicians.
Never install your ULT in an unprotected area. If the unit is exposed to moisture,
there is a danger of electric shock.
Your ULT must not be installed in a damp area or an area subjected to water spray,
as may reduce the degree of insulation and thereby cause electrical leakage or
electrical shock.
Never directly pour water into the unit. The water may cause electrical shock or short
circuit.

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Freezer User Manual
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Do not place heavy objects on top of the unit, as a falling object may cause injury.
Never use gas lines, water mains, telephone lines or lightning rods as the grounding
device for you ULT unit. This type of improper grounding may cause electric shock or
other danger.
Do not touch any electrical components, switches or power cord with wet hands.
Such actions may lead to electric shock.
When unplugging the power cord from the socket, please grip the plug itself and pull
it out. Do not pull on the power cord as this may strip the wires out of the plug
thereby causing electric shock and fire.
Should there be any Malfunction in the equipment, power off the unit and unplug the
power cord from the power supply. Continues operation in an abnormal condition
may result in electric shock and fire.
User must not dismantle, repair or modify the equipment. Such operations may result
in fire or personal injury.
Before any repair and maintenance of the freezer, please disconnect the power to
avoid electric shock or injury to personnel.
When repairing and maintaining your freezer, take precautions not to inhale any
chemicals or aerosols floating inside and outside the unit. They may be harmful to
your health.
If poisonous, radioactive or other harmful materials need to be stored in the unit, the
Equipment should be located in a safe zone. Improper use of the equipment with such
materials may harm the environment or operator’s health.
If the unit is not to be in use for a long period of time, make sure the power cord is
unplugged. Deteriorated insulation of the power cord may lead to electric shock or
fire.
The freezer needs to be disposed by specialized personnel.
Do not use nay unapproved electrical components with the freezer.
Never store flammable, explosive or volatile materials in the unit, nor use any
flammable spray near the unit, as this may cause an explosion or fire.
Never store corrosive chemicals with acid or alkaline properties in the unit, as this
can lead to damage to internal components of the unit.
Do not use any glass containers with the unit.
These containers may crack at low temperatures and cause injury.

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Do not climb on top of the unit or place any object on it. Falling equipment may cause
injury or property damage.
Do not use any hard objects such as nails and wires in opening or gaps such as air
ventilation ports. Accidental contact between a hard object and a moving part may
result in electric shock or injury.
Do not use electrical appliances inside the compartments of the appliance unless they
are recommended by the manufacturer.
The appliance must be positioned so that the plug is accessible.
The appliance must be placed on a solid and flat surface, or excessive vibration and
noise may occur when the appliance is operating.
If your cabinet is to be discarded, you must remove the door and leave the shelves in
place. This will reduce the possibility of danger.
If the supply cord is damaged, it must be replaced by the manufacturer, its service
agent or similarly qualified persons in order to avoid a hazard.
Users must not dismantle, repair or modify the equipment. Such improper
operations
may result in fire or personal injury.
CP/IP foaming materials are flammable and require professional processing for
disposal.
No modification of this equipment is allowed.
To avoid the risk of electric shock, this equipment must only be connected with a
grounded supply.
There should be at least 12.5 in space between the surrounding walls and the freezer
for ventilation.
Equipment cannot be run with increased levels of O2 , flammable gas or liquids.
After restarting your unit from a power outage or shutdown, ensure that all setting
are correct. Accidental changes in settings may damage the stored products.
In the event of a power outage and recovery, wait for at least 5 minutes before
turning the unit on again to avoid damage to compressors and refrigeration system.

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The air filter for the condenser should be cleaned regularly. A dirty filter could cause a
malfunction or the freezer temperature to rise.
During any repair operations, gloves should be worn to prevent getting injured by
sharp edges or corners.
Do not use bare hands to directly handle any stored products. The cold temperature
of products and the interior walls may cause frostbites.
Do not tilt the unit more than 45 degrees when moving it.
When moving the unit, please be cautious not to cause injury to personnel or damage
the unit.
Do not attempt to use the handle to lift or move the unit to avoid damaging the
freezer or injuring personnel.
Please open the lock first, then lift the handle.
Maximum weight per shelf is 50Kg/110lbs and weight of the entire unit should not
Exceed 200kg/440lbs. Heavy loads may cause damage to the shelving system.
Keep ventilation openings, whether the appliance enclosure or built-in structure, clear
Or obstructions.
Do not use mechanical devices or other means to accelerate the defrosting process,
unless preapproved by the manufacturer.
Do not damage refrigerant circuit.
Unauthorized opening of the top cover of the control cabinet prohibited to prevent
damage to the inside components or injury to the operator.
Turn the battery switch on before starting the unit; do not arbitrarily turn it off.
When the ULT unit has been placed in storage or not in use for a long time, its battery
should be tested for low capacity because the battery may have already released all
of its energy. Should this occur, please turn on the battery switch and run the unit for
about a week to fully charge up the battery.

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7.0 Usage Precautions
•When the unit operates normally, the unit frame at the front near the door may be
slightly warm; This phenomenon is normal because hot tubing is embedded there to
prevent condensation from forming on the frame.
•Before samples are loaded into the unit, make sure the unit temperature has
reached the set point then load the samples into the freezer in batches. Each batch
should not exceed 1/3 of the unit capacity, so that the temperature does not rise
while samples are being loaded.
•The temperature display indicates the temperature from the sensor location inside
the unit chamber, which may vary from the temperature at the center of the freezer
but will gradually reach the actual temperature of the freezer over time.
•Two access ports are installed in the back wall of the unit which can be used as the
pass-thru for thermocouple wires during testing and validation. Once all test wires
are through the access port, make sure that the gap in the port is sealed properly
with insulation materials. Failure to seal the access port may affect the operation of
the unit. The port ring in the outer wall can also accumulate frost and ice.
•When cleaning the unit, mild or neutral detergent solution should be used. Never
use a hard wire brush, acid, gasoline, powder detergent, polishing powder, or hot
water to clean the freezer, as these tools and materials can damage the paint
coating and plastic components. Never use gasoline or solution with volatile
chemicals to clean plastic or rubber parts.
•After the freezer runs for some time, a layer of frost usually forms on the interior
liner and inner doors. When this layer of frost becomes too thick, it can negatively
impact the refrigeration performance of the unit. Energy consumption can increase.
If the thickness reaches about .03 in / [5 mm], use the supplied scraper to remove
the frost.
•Before removing the frost, temporarily transfer the stored samples to another
freezer to prevent rising temperature from damaging the samples.
•Behind the interior walls, there are many refrigeration lines. Do not use a knife, an
ice pick, or a screwdriver to cut ice and frost. This may damage not only the liner
but also the refrigeration tubes.
•If the freezer is not in use for a period of time, turn off the power and backup
battery.
The power cord should be unplugged.

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Installation
8.0 Installation Environment
•Ambient temperature: 10°C to 32°C. The ideal temperature is 18°C to 25°C. If
necessary, use an air-conditioning system to achieve the requires ambient condition.
•Environment humidity: less than 80% RH. At an environment of 32°C, humidity
should be less than 57% RH.
•The intended location should be low in particulate count.
•The intended location should be vibration and shock free.
•Highest recommended elevation for safe usage: 2,000m above sea level.
•Input voltage: rated voltage ± 10%.
•An ULT is sensitive to its operating environment. If a unit is not installed in the
conditions mentioned above, it may not operate reliably.
•The unit is intended for indoor use only.
Do not dispose of electrical appliances as unsorted municipal waste, rather use
separate collection facilities. Contact your local government for information regarding
the collection systems available.

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9.0 Installation Location
For the equipment to achieve optimal operating conditions, an intended installation
location should satisfy the following requirements.
•Do not install the unit in a confined space. The doorway should be large enough for
the unit to freely be transferred in or out of the room if necessary. This is to allow
the unit to be repaired easily and timely to avoid damage to property.
•The location for installation should be flat and firm.
•There should be good ventilation and no direct sunlight.
•The freezer unit cannot share the same power socket with other equipment. The
power plug should be securely connected to the power socket.
•The power cord for the freezer should not be twisted or pinched.
•If the power cord needs to be extended, the cross-section of the extended cable
must not be no shorter than 2mm(14 gauge wire) and no longer than 3m(9.8ft).
•Before using the freezer, check the voltage supply. A voltage stabilizer to deliver
rated voltage ± 10% is recommended for areas in which the voltage is known to be
unstable. The voltage stabilizer should be rated at least 4000W.
•The freezer must be grounded.
•If the power socket is connected with a ground terminal, make sure to inspect it for
proper connection before using the equipment.
•Do not ground the freezer through gas lines, water mains, telephone lines and
lightning rods, as this may lead to electric shock.
•After installation, the power plug must be easily accessible to unplug in case of an
emergency. Nothing should block the ventilation port of the freezer.

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10.0 Before Use Preparations
1. Remove packing materials and
straps.
Remove all packing materials and
straps for transportation.
2. Check the supplied accessories
Check the items in the packing
box according to the packing list.
If they do not match each other,
please contact manufacturer.
3. Installation environment
These should be at least 12.5 in
space between the surrounding
walls and the freezer for
ventilation.
4. Adjust Leveling legs
Rotated the leveling legs clockwise
to extend them out to level the
unit to the floor and ensure that
the unit does not move while in
use.
5. Placement
After adjusting and cleaning the
unit, do not immediately connect
the power cord. The freezer needs
to be placed in its intended
location for at least 24 hours
before connecting the power to
ensure it will operate normally.

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11.0 Initial Power
When the unit is started for the first time, please follow the procedures below.
1. While keeping the unit empty, plug in the power cord to dedicated power socket
that meets all electrical requirements.
2. Connect the freezer to the power supply,
turn on the power switch located on the
side of the freezer (as in the illustration
on the right), and then turn on the battery
switch.
3. If the unit has a backup cooling system
(optional) turn off the backup system.
4. Test the unit: Do not load the unit with any
samples. Power up the unit to let it run down to
-60°C. Let it run at -60°C for 8 hours then lower
it to -80°C. Observe the unit performance for 24
hours for normal cycling to ensure that it is
working properly.
5. Once the unit is confirmed to be operating properly, it is ready to be loaded with
samples. Load the unit with samples in batches of less than 1/3 of the unit’s
capacity. Make sure that the unit is capable of cycling for more than 8 hours.
6. If the unit has a backup cooling system (optional), turn it on.

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•Your ULT unit should be cared for by trained personnel. Every 2 to 4 hours, the
Unit’s working status should be inspected and recorded on a daily basis.
Should there be a malfunction in the unit, the freezer temperature rises. If the
problems cannot be corrected in a short time, please remove the stored samples,
move them to another unit that meets the temperature requirements to avoid
potential damage to samples.
•Before putting samples in the freezer to be stored, first check that the freezer’s
temperature is at the desired setpoint to prevent damage or loss.
•All ultra-low temperature storage units are low temperature storage equipment.
It is not recommended to load excessive amounts of samples into the unit at one
time. Overloading may result in inadequate performance. The freezer and the
compressors can become overheated. Samples must be loaded in batches, while
gradually decreasing temperature setting. The process should be repeated until
the final temperature is reached.
•Do not use any unauthorized mechanical tools or other means to accelerate the
defrosting process.
•Do not damage the refrigeration circuit.
•Do not use any unapproved electrical components in the freezer.
12.0 Operation after a Power Outage
Your ULT freezer control settings are stored in its memory system. Should there be
power outage and recovery, the unit can resume its operation based on the previous
settings.
•In the level of a power outage and recovery, be sure to wait for at least 5
minutes before turning the unit on again to avoid damaging the compressors and
refrigeration system.
•If the unit is not in use for a long period of time, make sure the power cord is
unplugged. Deteriorated insulation of the power cord may lead to electric shock
or fire.

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13.0 Freezer Parts
13.1 Vertical Type

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13.2 Horizontal type
14.0 Control Panel

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15.0 How to Use the Freezer
15.1 Unlocking the freezer
Before adjusting the settings, you must unlock the freezer.
1. Press key, adjust the number to “06”.
2. Press and hold “Set” key for 5 seconds; the “locked” indicator turns off. The unit
is
unlocked mode and can be set.
3. Press “Set” key again to select mode for setting the inner temperature, high
temperature alarm and low temperature alarm; the corresponding indicator will
light up as each function is selected.
15.2 Setting the inner temperature
1. Unlocked mode, press “Set” key to select “Inner Temp”, the temperature display
flashes and displays the setting value.
2. Then, press key, adjust temperature setting value.
Temperature setting range: -10 to -86°C. Recommended temperature setting
range: -40 to -86°C.
3. Do not touch the unit for 10 seconds. After adjustment, the unit automatically
enters into locked mode and the temperature display stops flashing, which
means the value set has been entered into the computer. Otherwise, the setting
is invalid.
For example: Set the inner temperature to -80°C.
4. After setting the inner temperature, the high temperature alarm and low
temperature alarm will automatically adjust to proper values. If the user has
special requirements, follow the steps below to adjust the values manually.
15.3 Setting the high temperature alarm
1. In unlock mode, press “Set” key to select “High Temp. Alarm”, the temperature
Display Flashes and display the setting value.
2. Press key, adjust the high temperature alarm setting value.
Temperature setting range: at least +5°C above the inner temperature.
3. Do not touch the unit for 10 seconds. After adjusting, the unit automatically
enters into Locked mode and the temperature display stops flashing, which
means the value set has been entered into the computer. Otherwise, the
setting is invalid.
For example: If inner temperature is set to -80°C, setting the high temperature
alarm to -75°C is recommended.

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15.4 Setting the low temperature alarm
1. In unlocked mode, press “Set” key to select “low Temp. Alarm”, the temperature
Display flashes and display the setting value.
2. Then, press key, adjust the low temperature alarm setting value.
Temperature setting range: above -91°C and at most -5°C below the inner
temperature.
3. After adjusting, do not touch the unit for 10 seconds. The unit automatically
enters into Locked mode and the temperature display stops flashing which means
the value set has been input into the computer. Otherwise, the setting is invalid.
For example: Set the low temperature alarm to -91°C.
15.5 Setting the security code
When used for the first time, the security code to unlock the control is set at “06”.
1. In unlock mode, simultaneously press and hold “Set” key and “Silence” key for 5
seconds until the temperature display shows “06”.
2. Then press key, adjust the security code. The choices are from 05 to
30.
3. After adjusting, do not touch the unit for 5 seconds. The unit automatically
enters locked mode which means the setting is valid.
(Note: please remember your code.)
For example: Change the security code from 06 to 05.
15.6 Setting the startup timer
In order to reduce the startup power surge after a power outage, your ULT
incorporates a startup delay feature, which can alter startup sequence of the
compressors in the freezer.
1. In unlocked mode, simultaneously press and hold “Set” and key for 5
seconds, the temperature display shows the factory default value of the time
delay “01” (1 minute).
2. Then, press key, adjust the value. The choices are from 01 to 10 (1
to 10 minutes are available).
For example: Change the time delay from 1 minute to 5 minutes.

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15.7 Alarm test and low battery test
1. In unlocked mode, simultaneously press and hold key and “Silence” key for 5
seconds, the buzzer alarm sounds, and the alarm indicator flashes.
2. At this time the 6-second battery current test is initially implemented.
If the battery capacity is low, the low battery indicator will flash 6 times. If the
Battery capacity is normal, the indicator will not light up or flash.
The choices are from 05 to 30.
3. After 6 seconds, the alarm test starts up.
All indicators will light up for 6 seconds without flashing and all electric display
Windows will indicate “8” for 6 seconds, which means all display functions are
working properly.
15.8 Setting the USB storage time
1. In unlocked mode, press and hold key for 3 seconds; the temperature
display steadily shows ”1P”.
(“1P/2P/3P/4P/5P” stands for “Years/Months/Days/Hours/Minuets”, respectively)
2. Press ‘Set” key, the temperature display shows years and flashes (default value:
10).
Press key, select years. The choices are from 10 to 99. If you want
to set the Year 2013, select 13.
Press “Set” key to save the changes; then, the temperature display steadily
shows “2P”.
Follow the procedure above to set “2P”, “3P”, “4P” and “5P” respectively, and
press “Set” key to save the changes.
The temperature display will then show “1P” again, at which point you can reset
1P to 5P.
For example: Set the time as 08:30, August 16, 2019.
3. After the settings are completed, press and hold “Set” for 5 seconds; the
changes will be Saved and exit the settings automatically. The temperature
display will then normally show the inner temperature.

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During the above procedure setting 1P to 5P, you can use key to adjust the
setting Individually.
For example: The temperature display shows 1P (Years), if you do not need to change Years
(1P) and Months (2P), you can adjust Days (3P) directly.
1. Press key to select Days (3P).
2. Press “Set” key, the temperature display shows the date and flashes.
3. Press key to adjust the current date.
4. Press “Set” key to save the change. Then, the temperature display will show
Hours (4P).
5. If you need to adjust the Hours, press “Set” key to change the setting.
If no adjustments need to be made, press key; the display shows Minutes
(5P), changes can then be entered.
6. After having finished the settings, press and hold “Set” for 5 seconds, the
changes will be saved and the settings menu will be automatically exited from.
The temperature display should show the inner temperature.
During the above procedure setting 1P to 5P and after finishing adjustment of any of the
settings, and if no other settings need to be changed, you can press and hold “Set” key for 5
seconds to save the changes and exit the settings.
15.9 Exporting USB data
1. In unlocked mode, insert the USB flash drive. The temperature display USB,
which means data is being exported.
2. When the temperature display shows ALL and exporting of the data is finished,
Please remove the USB flash drive.
the system memory can save data for up to 10 years.