Omni Bead Ruptor Cryo Cooling Unit User manual

Bead RuptorTM
Cryo Cooling Unit
User Manual

Data herein has been veried and validated. It is believed adequate for the intended use of
the instrument. If the instrument or procedures are used for purposes over and above the
capabilities specied herein, conrmation of the validity and suitability should be obtained;
otherwise OMNI International, a PerkinElmer company does not guarantee results and
assumes no obligation or liability. This publication is not a license to operate under, or a
recommendation to infringe upon, any process patents.
This product is warranted to be free from defects in material and workmanship for a
period of ONE YEAR from the date of delivery. OMNI International, a PerkinElmer company
will repair or replace and return free of charge any part which is returned to its factory
within said period, transportation prepaid by user, and which is found upon inspection to
have been defective in materials or workmanship. This warranty does not include normal
wear from use; it does not apply to any instrument or parts which have been altered by
anyone other than an employee of OMNI International, a PerkinElmer company nor to
any instrument which has been damaged through accident, negligence, failure to follow
operating instructions, the use of electric currents or circuits other than those specied on
the plate axed to the instrument, misuse, or abuse. OMNI International, a PerkinElmer
company reserves the right to change, alter, modify, or improve any of its instruments
without any obligation whatever to make corresponding changes to any instrument
previously sold or shipped.
THE FORGOING OBLIGATION IS IN LIEU OF ALL OBLIGATIONS AND LIABILITIES INCLUDING
NEGLIGENCE AND ALL WARRANTIES OF MERCHANTABILITY OR OTHERWISE, EXPRESSED
OR IMPLIED IN FACT OR BY LAW, AND STATE OUR ENTIRE AND EXCLUSIVE LIABILITY
AND BUYERS EXCLUSIVE REMEDY FOR ANY CLAIM OF DAMAGES IN CONNECTION WITH
THE SALE OR FURNISHING OF GOODS OR PARTS, THEIR DESIGN, SUITABILITY FOR USE,
INSTALLATION, OR OPERATION. OMNI INTERNATIONAL, A PERKINELMER COMPANY WILL
IN NO EVENT BE LIABLE FOR ANY SPECIAL OR CONSEQUENTIAL DAMAGES WHATSOEVER,
AND THEIR LIABILITY UNDER NO CIRCUMSTANCES WILL EXCEED THE CONTRACT PRICE FOR
THE GOODS FOR WHICH LIABILITY IS CLAIMED.
This product has been engineered for safety; however, basic safety precautions and
common sense must always be demonstrated when using any electrical product.
- DO NOT attempt to modify any part of this product.
- DO NOT allow the machine to be submerged in any liquid.
- DO NOT use in any setting other than an indoor laboratory.
- Use this product only for its intended purpose.
- DO NOT use attachments not recommended by the manufacturer.
- DO NOT operate the product if it is damaged in any way.
- Keep this product away from heated surfaces.

Overview
1.
2.
3.
4.
5.
6.
7.
8.
The Bead Ruptor Cryo Cooling Unit consists of the following:
Description Quantity
Bead RuptorTM Cryo Cooling Unit 1
Insulated Cryo Hose 1
Bead RuptorTM Cryo Lid 1
External Thermometer 1
Compressed Air Hose 1
Tool Kit 1
User Manual 1
Not Included
Hose Barb - 3/8” or equivalent 1
1. Lid
2. Airow Control Knob
3. Insulated Cryo Hose
4. Bead RuptorTM Cryo Lid
5. Cold Air Outow
6. Compressed Air Input
7. Compressed Air Hose
8. External Thermometer

Installation
Removing the Bead RuptorTM Bead Mill Homogenizer Lid
Locate Lid Hinges.
1.
2.
Lift Lid and remove 4 screws using the provided Allen Key. Remove Lid
Replace with Bead RuptorTM Cryo Lid (Black)
3.
4.
Replace the four screws and tighten using the provided Allen Key.
Align black Cryo lid onto the Bead RuptorTM.

Installation
Install the External Thermometer
Position the Thermometer Probe under the Bead Ruptor lid as shown,
using the Couple Clip to secure the thermometer probe wire under the
Bead Ruptor lid.
Place remaining Couple Clips as shown. Guide
the thermometer probe wire through the clips.
Plug the thermometer probe wire into the
External Thermometer.
NOTE: The Digital Thermometer has been
preset to Celsius with alarms to be triggered if
temperatures reach below -10C or over 70C.
1.
2.
3.
Couple clip
Thermometer probe
wire
Thermometer probe

Installation
Install Intake and Output Hoses
1.
2.
3.
NOTE: Compressed ISO8573 Class 2 or better air is required to use the Bead
RuptorTM Cryo unit: Total oil < 0.1 mg/m3, vapor pressure dew point < -50C. The
compressed air hose can be tted to a standard medical air regulator using a 3/8”
hose clamp.
Recommended Dry Air Guidelines
• Obtain a high-pressure cylinder of Medical Air. Medical Air is a blend of
nitrogen and oxygen that contains virtually no traces of oil or water vapor.
OR
• Between an air compressor and the Bead RuptorTM Cryo Unit inlet, use a
refrigerated air dryer or desiccant dryer. These systems are specied in the
lowest dew point that can be reached.
• DO NOT use inlet pressure below 55 PSI or above 120 PSI.
Connect the hose from air supply to the rear of
the Bead RuptorTM Cryo unit.
Note: An optional hose with a “quick connect”
coupling has been included with the Bead
RuptorTM Cryo.
An additional tting is required to connect the
hose to the air supply.
Hose Barb - 3/8” or equivalent.
Connect the straight end of the Insulated Cryo
Hose to the Bead RuptorTM Cryo Unit.
Connect the 90 elbw end of the Insulated Cryo
Hose to the Bead RuptorTM Homogenizer Lid.

Operation
Liquid Nitrogen
Pre - Cooling
1.
3.
2.
WARNING: Wear eye, face, hand and skin protection when working with liquid
nitrogen. Operate in a well ventilated area.
PRE-COOLING - follow pre-cooling procedure before homogenizing samples.
NOTE: Do not load samples into the Bead Ruptor processing chamber prior
to pre-cooling.
- Purge air lines by running dry air for 30
seconds at the MAX setting.
- Turn knob to the Off position.
Close the lid of the Bead RuptorTM Cryo unit and turn the air ow control
knob counterclockwise to begin the ow of air into the processing chamber.
Pre-cool the Bead RuptorTM Homogenizer processing chamber to 0C.
WARNING: DO NOT pre-cool the processing chamber below 0C.
Add 0.5 L or 16 oz. of liquid nitrogen to the
chamber, ll to 3 inches (7.6 cm) below the top
of the chamber.
NOTE: The copper coils in the chamber must
be completely covered by liquid nitrogen.

Operation
Liquid Nitrogen
Homogenizing Samples
Add 0.5 L or 16 oz. of liquid nitrogen to the
chamber of the Bead RuptorTM Cryo unit, ll to
3 inches (7.6 cm) below the top of the chamber.
NOTE: The copper coils in the chamber must
be completely covered by liquid nitrogen.
CAUTION: 55-120 PSI is the recommended air pressure for use with the
Bead RuptorTM Cryo. Pressure above 120 PSI could damage internal
components.
Close the Bead RuptorTM Homogenizer lid.
- Load samples into the Bead RuptorTM
Homogenizer.
- Install Finger Plate
- Set the desired time, speed dwell and
number of cycles on the Bead RuptorTM
Homogenizer.
WARNING: BR Cryo is compatible with
1.5 mL, 2 mL and 7 mL tubes only.
Ensure that the knob of the Bead RuptorTM
Cryo is in the o position and all hoses are
connected correctly.
1.
2.
3.
4.

Operation
Liquid Nitrogen
Homogenizing Samples
WARNING: Avoid direct skin contact with Bead RuptorTM Cryo and Bead
RuptorTM Homogenizer components after exposure to liquid nitrogen. Always
use protective gloves.
Turn Airow control knob counter clockwise
to begin cooling.
Allow air to ow until the External
Thermometer reads 0C.
Once the temperature has reached 0C,
press RUN on the Bead Ruptor to begin
homogenization.
When the cycle has ended:
- Turn o compressed air supply
- Turn o Bead RuptorTM Cryo
- Open the lid of the Bead RuptorTM
Homogenizer and remove samples.
CAUTION: Allow liquid nitrogen to evaporate
before attempting to move the Bead RuptorTM
Cryo unit.
5.
6.
7.
8.

Operation
Dry Ice
Pre-Cooling
1.
3.
2.
Liquid nitrogen will provide the best results.
However, dry ice and ethanol may be used.
WARNING: Wear eye, face, hand and skin protection when working with Dry Ice.
Operate in a well ventilated area.
PRE-COOLING - follow pre-cooling procedure before homogenizing samples.
NOTE: Do not load samples into the Bead Ruptor processing chamber prior to
pre-cooling.
- Purge air lines by running dry air for 30
seconds at the MAX setting.
- Turn knob to the Off position.
Close the lid of the Bead RuptorTM Cryo unit and turn the air ow control
knob counterclockwise to begin the ow of air into the processing chamber.
Pre-cool the Bead RuptorTM Homogenizer processing chamber to -5C.
WARNING: DO NOT pre-cool the processing chamber below -5C.
- Add 0.5 L (16 0z.) of dry ice to the Bead RuptorTM
Cryo chamber.
- Add 0.5 L (16 oz.) of ethanol to the Bead RuptorTM
Cryo chamber and let the mixture sit for five minutes
to allow the coil to cool.
NOTE: The copper coils in the chamber must be
completely covered by the ethanol and dry ice.
WARNING: Do not ll more than 3/4 of the
chamber volume as leaking may occur.