Orange Photonics LIGHTLAB User manual

INSTRUCTION
MANUAL
LIGHTLAB™ CANNABIS POTENCY ANALYZER
BY ORANGE PHOTONICS
Version 1.3.3

INTRODUCTION

INTRODUCTION
Contents
Welcome_________________________________________________________________________________________________ 5
LightLab Packing List___________________________________________________________________________________ 6
LightLab Technology Overview ________________________________________________________________________ 7
What Does LightLab Measure?_________________________________________________________________________ 8
1. LightLab Setup_______________________________________________________________________________________ 10
2. Sample Analysis- Flower____________________________________________________________________________ 15
3. Sample Analysis- Concentrates_____________________________________________________________________24
4. Sample Analysis- Trim______________________________________________________________________________34
5. Sample Analysis- Wet Flower ______________________________________________________________________ 43
6. Sample Analysis- Raffinate _________________________________________________________________________53
7. Sample Analysis- Infused Product _________________________________________________________________ 62
8. Sample Analysis- Custom ___________________________________________________________________________ 72
9. Shutting Down_______________________________________________________________________________________ 82
10. Scale Calibration ___________________________________________________________________________________87
11. LightLab Settings __________________________________________________________________________________ 89
Replace Solvent.........................................................................................................................................89
Replace Column........................................................................................................................................91
Flush System..............................................................................................................................................93
Flushing Forward ________________________________________________________________________ 94
Flushing Backward ______________________________________________________________________ 95
Clearing Sample Input___________________________________________________________________ 96
Change Date/Time ..................................................................................................................................99
Export Data ................................................................................................................................................99
Reset Counter.........................................................................................................................................100
Device Name...........................................................................................................................................100
Support.....................................................................................................................................................101
Update.......................................................................................................................................................101
Sounds on/off.........................................................................................................................................102

INTRODUCTION
ProMode on/off.....................................................................................................................................102
Moisture Correction on/off...............................................................................................................102
12. Troubleshooting _________________________________________________________________________________ 103
Zero Timeout..........................................................................................................................................103
Description______________________________________________________________________________103
Causes and Fixes ________________________________________________________________________103
Air Bubbles Detected...........................................................................................................................103
Description______________________________________________________________________________104
Causes and Fixes ________________________________________________________________________104
Result Confidence is Marginal/Result confidence is Poor....................................................105
Description______________________________________________________________________________105
Causes and Fixes: _______________________________________________________________________105
Flow was out of range.........................................................................................................................107
Description______________________________________________________________________________107
Causes and Fixes: _______________________________________________________________________107
High Absorbance Sample...................................................................................................................108
Description______________________________________________________________________________108
Causes and Fixes ________________________________________________________________________108
Power on Self Test (POST) Fail ........................................................................................................109
Description______________________________________________________________________________109
Causes and Fixes ________________________________________________________________________109
13. Compliance and Safety Information____________________________________________________________ 113
Safety Information ...............................................................................................................................113
Compliance information ....................................................................................................................113
14. Contact Information _____________________________________________________________________________ 115
Support Contact Information...........................................................................................................115
Customer Dashboard...........................................................................................................................115
General Inquiries ..................................................................................................................................115
Sales Support..........................................................................................................................................115

INTRODUCTION

INTRODUCTION
Welcome
Welcome and thank you for choosing Orange Photonics.
Your LightLab cannabis potency analyzer comes to you fully calibrated and ready to use.
We are proud to serve established cannabis industry leaders and newcomers in support
of greater efficiency and higher quality products through data.
On behalf of the entire Orange Photonics team, we look forward to working with you.
Sincerely,
The Orange Photonics Team

INTRODUCTION
LightLab Packing List
The first time you use the LightLab unpack the components on a clean, flat surface and
familiarize yourself with each item.
Packing list:
Items
Units
LightLab Analyzer
1
Extraction Shaker and Power Supply
1
Fluidics Hardware
1
Flushing Hardware
1
Grinder
1
Instruction Manual
1
Power Supply
1
Sample Input Cover
1
Sample Warmer
1
Scale - 20g
1
Scale Calibration Weight
1
SD Card
1
SD Card Reader
1
Shaker Straps
2
Tweezers
1
USB Cable
1

INTRODUCTION
LightLab Technology Overview

INTRODUCTION
What Does LightLab Measure?
LightLab uses a combination of chromatography (chemical separation based on
molecule polarity) and spectroscopy (light based chemical analysis) to provide accurate
analysis of several components in complex mixtures. Your LightLab is factory calibrated
and will begin providing results directly out of the box for 6 major cannabinoids. The
following is an explanation of the results the LightLab generates along with an example
results screen:
The following is a list of results shown and their meaning:
THCA: Tetrahydrocannabinolic Acid. This is the “acidic” form of tetrahydrocannabinol
(THC). Cannabis plants naturally produce THCA and is the primary cannabinoid that
will be present in most cannabis strains. Typically, plants have 10-20% THCA. A higher
THCA number means a more potent plant.
Δ9THC: Delta 9 Tetrahydrocannabinol. This is the “active” or “neutral” form of THC.
This is the primary psychoactive cannabinoid seen in cannabis plants. Plants do not
directly produce Δ9THC. Instead, THCA is converted into Δ9THC through a process
called decarboxylation. Decarboxylation occurs when the plant is smoked, otherwise
heated or exposed to light. Typically, plants have 0-5% Δ9THC. High levels of Δ9THC in
plant material indicate the plant may not have been stored or cured well or may be old.
Total Potential Δ9THC: This number indicates the total quantity of Δ9THC if the
sample was completely decarboxylated. Decarboxylation is the conversion of THCA to
Δ9THC in the presence of heat or light. During the decarboxylation process, a CO2
molecule is released, so a THCA molecule will weigh less once it is converted to Δ9THC.
For that reason, the total “potency”, or how much psychoactive Δ9THC a user would be

INTRODUCTION
dosed with requires a conversion factor. The “total potential Δ9THC” factors in the loss
of weight of THCA when converting to Δ9THC. The equation used is as follows:
Total Potential Δ9THC= 0.877*THCA + Δ9THC
This number is an indication of the overall “potency” of the sample.
Total THC: This number is the sum of THCA + Δ9THC and is typically used to indicate
the overall THC cannabinoid content present in a sample. Note this number will always
be greater or equal to the “Total Potential Δ9THC”. We recommend when considering
overall potency to use Total Potential Δ9THC instead of total THC.
CBDA: Cannabidiolic Acid. CBDA is the CBD analog to THCA. It is the acidic from of CBD
that plants produce. Typical non-CBD specific strains will have 0-2% CBDA. CBD
Specific plants typically contain 5-20% CBDA. CBDA is not psychoactive.
CBD: Cannabidiol. CBD is the neutral form of CBDA. Cannabis plants do not create CBD
directly, however this cannabinoid can be formed through the same decarboxylation
process described above.
CBN: Cannabinol. CBN is a breakdown component of Δ9THC. It is mildly psychoactive
and also sedative. Fresh cannabis plants typically show no CBN. Very old plants may
contain 0-5% CBN. CBN can also be generated during extraction or distillation, and
commonly occurs at 0-5% levels in extracted samples. More CBN is typically
undesirable and is an indication of too much heat or exposure to environmental factors.
CBGA: Cannabigerolic Acid. CBGA is a precursor molecule to THCA and CBDA. When a
plant produces cannabinoids, it always produces CBGA first, then an enzymatic process
converts CBGA to THCA and/or CBDA. CBGA can be used as an indicator of harvest
readiness. If >1% CBGA is present in a sample, it typically means the plant can continue
to produce active cannabinoids. A CBGA value of <1% is typically desirable. Plants
commonly contain between 0-4% CBGA.

LIGHTLAB SETUP
1. LightLab Setup
1. Open LightLab and remove cap baton and power supply.
2. Plug in LightLab if power is available (LightLab has an 8-hour battery). Do not turn
the LightLab on yet. Note: LightLab has two charge settings on the charger (0.9A
and 1.8A). We recommend using the 1.8A setting for fastest charge time.

LIGHTLAB SETUP
3. Remove waste cap from baton and place on waste container
4. Remove solvent cap from baton and place on solvent container
5. Connect fluid lines from bottles to LightLab.

LIGHTLAB SETUP
6. Lift column holder.
7. Remove Selective Separation Column caps and insert into column holder.

LIGHTLAB SETUP
8. Close column holder onto Selective Separation Column.
9. Turn on instrument. A systems check will run. Once complete, the LightLab will ask
to start the warmup.

LIGHTLAB SETUP
10. Press “Start Warmup” to begin the warmup process. NOTE: the warmup can be
skipped if the solvent caps and fluid connections have not been disconnected and
the system has been run recently (within one hour).

SAMPLE ANALYSIS - FLOWER
2. Sample Analysis- Flower
Flower analysis should be used with any dried and cured cannabis flower material.
1. Select the type of sample to be measured (flower for this section). NOTE: if tests
remaining is zero, you will be prompted to replace the column, see Section
Replace Solvent
2. If Moisture Correction is enabled, enter the moisture content of the sample to be
run. If the moisture is not known, enter 0 to continue.

SAMPLE ANALYSIS - FLOWER
3. Add sample tags to the sample record if desired. These tags will be saved along
with the sample results and can be accessed later.
4. Prepare at least 100mg of sample by grinding the sample with the included
grinder. More than 100mg can be ground to get a homogenous sample if desired.
We recommend grinding more than 1000mg.
5. Place scale on level surface and turn on.

SAMPLE ANALYSIS - FLOWER
6. Check calibration of scale by placing 10g calibration weight on scale. Results
should be between 0.997 and 10.003. If not proceed to Section 4 to recalibrate
scale.
7. Place a vial cap on the scale and press “tare”. Ensure scale now reads zero with
cap resting on it. Press forward arrow on the LightLab screen.

SAMPLE ANALYSIS - FLOWER
8. Add 100mg (+/- 10mg) to the scale. Make sure all sample is within inner circle
of cap. Press forward arrow on the screen. NOTE: if moisture content was set to
a value greater than 25%, add 500mg (+/- 50mg) to the scale.
The sample may alternatively be weighed in a small weigh boat or the sample
vial. If a weigh boat is used, we recommend placing the whole weigh boat into
the sample vial so that no trichomes are lost during the transfer to the vial.
9. Type in the exact sample reading on the scale. 90-110mg is an acceptable range.
Press forward arrow on the screen. Note 100mg is 0.100g.
10. Pour sample into the vial. Ensure all the sample is inside the vial and not
trapped in the vial cap threads. Press forward arrow on the LightLab screen.

SAMPLE ANALYSIS - FLOWER
11. Connect the syringe to the solvent cap and pull plunger up to fill syringe. Fill
syringe with at least 10ml of solvent.
12. Invert the syringe so that the bubble floats to the tip. Depress the plunger to
remove the air bubbles and get the syringe volume to exactly 10ml. A tissue may
be used to catch any excess solvent.
NOTE: It is important to ensure the syringe has exactly 10ml of solvent for
accurate results.