PBS Biotech PBS 0.1 MAG User manual

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Single-Use Bioreactors with
Vertical-WheelTM Technology
User Manual
MINI
BIOREACTORS
TM
MAG MAG

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The PBS MINI Bioreactor System is a single-use cell culturing
device, intended primarily for culture of mammalian cells in
therapeutic protein, vaccine, gene therapy, and cell therapy
applications. This system consists of a MINI Single-Use Vessel
with the patented Vertical-Wheel™ Impeller and a non-disposable
MINI Universal Base Unit that holds the vessel. The Base Unit can
accommodate either a PBS 0.1 Single-Use Vessel or a PBS 0.5
Single-Use Vessel.
The agitation rate in the Single-Use Vessel is controlled by
the speed adjust dial on the MINI Bioreactor Base Unit and is
displayed on the digital speed display next to the dial. To regulate
temperature, pH, and dissolved oxygen (DO) levels in the culture,
the system must be placed in a warm room or an incubator with
appropriate temperature and gas controls.
The MINI Bioreactor Base Unit may be powered by an AC power
source, either at a voltage of 120V or 240V depending on the
model. It is environmentally sealed for use in up to 100% humidity
(condensing) condition and is constructed to be cleanable with
70% isopropyl alcohol, or equivalent.
The PBS 0.1 and PBS 0.5 Single-Use Vessels are primarily
constructed from injection-molded polycarbonate, and they are
sterilized by gamma radiation to be ready-for-use by the customer.
All product-contact surfaces in the Single-Use Vessels consist
of components certied to be Animal-Derived Component Free
(ADCF) and compliant to USP Class VI, ISO 10993, or equivalent.
About the PBS MINI
PBS Biotech Inc.
© 2017 PBS Biotech, Inc. All rights reserved. All text, images, graphics and other materials in
this manual are subject to the copyright and other intellectual property rights of PBS Biotech
Inc. Under the copyright laws, this manual may not be copied, in whole or in part, without
written consent of PBS Biotech, Inc. The marks appearing in this manual including, but not
limited to: (i) PBS Biotech and the PBS Biotech logo; (ii) Vertical-Wheel™ Bioreactor and its
logo, emblem, slogans and model names and designs are trademarks of PBS Biotech Inc.,
and registered in the U.S. and other countries. Every eort has been made to ensure that the
information in this manual is accurate. PBS Biotech is not responsible for printing or
clerical errors.

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PBS MINI User Manual IL00266 rev C
Universal Base Unit
Provides agitation power and
illumination to either Single-Use
Vessel size.
PBS 0.1 Single-Use Vessel
Contains and mixes up to 100 mL
of cell culture at a controlled speed
with the integrated Vertical-Wheel™
Impeller.
PBS 0.5 Single-Use Vessel
Contains and mixes up to 500 mL
of cell culture at a controlled speed
with the integrated Vertical-Wheel™
Impeller.
Vent Cap
Contains a 0.22-micron sterilizing-
grade lter membrane for gas
exchange. Can be removed from the
Single-Use Vessel for liquid handling.
Vertical-Wheel™ Impeller
Rotates vertically inside the
Single-Use Vessel to facilitate
ecient mixing, in combination
with the unique shape of the
bioreactor vessel.
Speed Adjust Dial
Allows variable speed control of the
impeller, from 5 RPM to 100 RPM.
Digital Speed Display
Oers enhanced visualization of
microcarrier suspension and cell
culture in the Single-Use Vessel.
White LED Lights
Oer enhanced visualization of
microcarrier suspension and cell
culture in the Single-Use Vessel.
LED Light Switch
Turns the LED lights on or o.
External DC Power Cord
(not shown)
Supplies power to the Base Unit from
an A/C power source. The power
supply on the cord should be kept
outside of the incubator by running
the thin DC extension cable through
the incubator door seal.
SEL and RST Buttons
Reserved for factory setup and
programming. No eect on function
of PBS MINI.
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1. Place a PBS MINI Bioreactor Base Unit in an incubator or in a
warm room and plug it in to an electrical outlet.
2. Add cell culture medium or other applicable solution into a PBS
0.1 or PBS 0.5 Single-Use Vessel by removing the Vent Cap.
Perform this operation in a biosafety cabinet or other controlled
environment to maintain sterility in the Single-Use Vessel.
3. Conrm the Speed Adjust Dial is set to 0 RPM.
4. Install the Single-Use Vessel in the Base Unit by matching the
back of the Vessel with the dovetail groove on the Base Unit
and sliding it down the groove. Note that there will be a sizeable
vertical gap between the bottom of the PBS-0.1 Single-Use
Vessel and the Base Unit even when it is installed properly.
5. Turn the Speed Adjust Dial to set the agitation rate in the Vessel
to deliver the required process condition.
6. Turn on the LED lights to visualize the Vertical-Wheel™ Impeller
rotation, as needed. The LEDs should be turned o whenever
possible when working with a light-sensitive process.
Installing the PBS MINI

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PBS MINI User Manual IL00266 rev C
• To keep the cells evenly mixed during
sampling, a Base Unit should be used
in the biosafety cabinet to control
agitation rate.
• To remove sample from the very bottom
of the vessel, the Vertical-Wheel™
Impeller must be rotated so the vanes
are at the top and bottom of the wheel,
as illustrated.
Maintenance and Services
• The only required maintenance is to regularly clean the Base Unit
with a solution of 70% isopropyl alcohol, or equivalent.
• The Base Unit has no user-serviceable parts. Do not attempt to
disassemble the Base Unit.
Removing a Sample from the PBS MINI

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Issue with Powering Up
1. Verify that the Base Unit is plugged into a functional
electrical outlet.
2. If you are using a power strip or a surge protector, verify that
its switch is also turned on.
3. Unplug the Base Unit from the outlet and re-plug it into
the outlet.
Issues with Agitation
Note: The display gets updates from the motor several times
per revolution. This may result in the displayed rotational speed
oscillating between a few RPM.
1. Verify that the Base Unit is powered up properly.
2. Verify that the Single-Use Vessel sits all the way down in the
Base Unit to ensure proper magnetic coupling.
3. Verify that the Speed Adjust Dial is not in the o position.
4. Visually determine any faults in the Vertical-Wheel™ Impeller:
• Is there any visible damage to the impeller?
• Does the impeller rotate smoothly in its bearings?
Issues with LED Lights
1. Verify that the Base Unit is powered up properly.
2. Push down rmly on the LED Light Switch.
For further questions, please contact Customer Service at
customer[email protected].
Troubleshooting the PBS MINI

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FEATURES PBS 0.1 MAG PBS 0.5 MAG
Universal Base Unit
Size
• Width
• Depth
• Height
• Weight
5.2 in (13.3 cm)
6.4 in (16.3 cm)
6.1 in (15.5 cm)
2.5 lb (1.1 kg)
Vertical-Wheel™ agitation:
• Mechanism
• Speed range
• Speed control
• Display
Magnetic coupling
5-100 RPM
On/o, speed adjust dial, RPM
Digital display of agitation (RPM)
Environmental:
• Operational temperature
• Operational humidity
Ambient to 40°C
0% to 100% (condensing)
Electrical 120V/240V Universal external
detachable power supply
Safety and regulatory Low voltage operation,
NRTL, CE certied external power supply
Illumination of vessel Two embedded white LEDs
Single-Use Vessel
Size
• Width
• Depth
• Height
• Height (base with vessel)
2.6 in (6.6 cm)
1.8 in (4.6 cm)
5.1 in (13.0 cm)
7.8 in (19.8 cm)
4.1 in (10.4 cm)
2.4 in (6.2 cm)
7.9 in (20.1 cm)
10.0 in (25.4 cm)
Working volume range 20 – 100 mL 100 – 500 mL
Number of ports 1 2
Gassing Diusion through 0.22 micron lter
on vent cap
Liquid transfer Port with screw-on vent cap
Product contact materials All ADCF and USP Class VI/ ISO 10993
compliant
Technical Highlights for PBS MINI

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[phone]
www.pbsbiotech.com