PerkinElmer 200 UV/VIS Series User manual

Series 200 UV/VIS Detector
User’s Manual

Release History
Part Number Release Publication Date
09936504 BJune 2000
Any comments about the documentation for this product should be addressed to:
User Assistance
PerkinElmer
710 Bridgeport Avenue
Shelton, Connecticut 06484-4794
U.S.A.
Or emailed to: [e-mail]
Notices
The information contained in this document is subject to change without notice.
Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes
no warranty of any kind with regard to this document, including, but not limited to, the
implied warranties of merchantability and fitness for a particular purpose.
PerkinElmer shall not be liable for errors contained herein for incidental consequential
damages in connection with furnishing, performance or use of this material.
NOTE: TotalChrom is the updated version of the software previously marketed as
Turbochrom. This product is compatible with TotalChrom and Turbochrom version 6.1.x.
Copyright Information
This document contains proprietary information that is protected by copyright.
All rights are reserved. No part of this publication may be reproduced in any form
whatsoever or translated into any language without the prior, written permission of
PerkinElmer, Inc.
Copyright © 2003PerkinElmer, Inc.
Produced in the USA.
Trademarks
Registered names, trademarks, etc. used in this document, even when not specifically
marked as such, are protected by law.
PerkinElmer is a registered trademark of PerkinElmer, Inc.
TotalChrom Client/Server and TotalChrom Workstation are trademarks of PerkinElmer, Inc.
Turbochrom is a trademark of Applera Corporation.

Electromagnetic Compatibility (EMC)
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S - 1
Electromagnetic Compatibility (EMC)
United States
This product is classified as a digital device used exclusively as industrial, commercial, or
medical test equipment. It is exempt from the technical standards specified in Part 15 of the
FCC Rules and Regulations, based on Section 15.103 (c).
Europe
All information concerning EMC standards will be in the Declaration of Conformity and
these standards will change as the European Union adds new requirements.
S
Safety and Regulatory
Information

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Safety and Regulatory Information
Your Series 200 UV/VIS Detector was manufactured and tested at the PerkinElmer facility
in Shelton, Connecticut. The following Safety and RegulatoryInformation is provided for
your information and should be read before proceeding to the installation section.
Symbols and Conventions Used in this Manual
The following graphic symbols and graphic text formats are used in this manual to set apart
important safety information.
A warning indicates an operation that could
cause
personal injury
if precautions are not
followed.
A caution indicates an operation that could
cause
instrument damage
if precautions are
not followed.
Notes emphasize significant information in a
procedure or description.

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Symbols Used on the Instrument
There are two different types of warning symbols that appear on the instrument.
Additional graphic symbols used on the instrument are the following:
Figure S-1. Additional symbols used on the instrument.
This symbol indicates an operation (or condi-
tion on the instrument) that could cause
per-
sonal injury
if precautions are not followed.
This can be any type of hazard.
When you see this symbol, refer to the safety
pages in the manual for further information.
Consulter les documents d’accompagnement.
This symbol indicates the danger of electric
shock, if precautions are not followed, due to
the presence of high voltage.
Attention. Risqué de choc électrique.
Alternating voltage.
Protective conductor terminal.
Indicates the off position of the main power switch.
Indicates the on position of the main power switch.
This symbol indicates a hot surface.

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Warnings/Labels on the Instrument (Label Location on Rear Panel)
Figure S-2. Location of labels on the Series 200 UV/VIS rear panel.
Label No.
(in photo)
Explanation
1
Safety information
2
Instrument name, part number, serial number, CE mark
3
Fuse, voltage, frequency and power information
4
CAUTION
Do not restrict air intake or exhaust
Attention: N’obstruez pas l’arrivée ou l’évacuation de l’air.
5
WARNING
Grounding circuit continuity is vital for safe operation of equipment.
Never operate equipment with grounding conductor disconnected.
Débrancher le cordon d’alimentation avant d’ouvrir da continuité des
masses est essentielle. Pour un fonctionnement sans danger. Ne jamais uti-
liser l’équipment si le fil de terre n’est pas raccordé.
6
WARNING
For protection against fire hazard, replace only with same type and rating
of fuse.
Afin d’assurer la protection contre les risques d’incendie, remplacer
uniquement par un fusible de même type et de même courant nominal.
7
WARNING
To avoid electrical shock, disconnect power supply before changing fuse.
Disconnect supply whenever cover is removed.
Avertissement: Afin d’eviter les risques d’électrocution, mettez l’appareil
hors tension avant de changer le fusible. Mettez l’appareil hors tension
chaque fois que vous retirez le couvercle.
1
2
3
7
6
5
4

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Warning for Front Panel
You may need to open the front panel to replace a lamp. Chapter 7,
Maintenance
, explains
this procedure. The following illustration contains safety information for the procedure.
Figure S-3. Front Panel with door open (for a lamp replacement)
Turn the Detector power switch to the OFF
position, and unplug the AC line cord from the
AC outlet before loosening the Allen bolt.
Allen bolt and Allen wrench
used in lamp replacement
procedure.
Location of label

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Electrical Warnings – General
The instrument should be used according to
the instructions provided in this manual. If
used otherwise, the protection provided by the
instrument may be impaired.
Connect the instrument to an AC line power
outlet that has a protective ground connection.
To ensure satisfactory and safe operation of
the instrument, it is essential that the protec-
tive ground conductor (the green/yellow lead)
of the line power cord is connected to true elec-
trical ground. Any interruption of the protec-
tive ground conductor, inside or outside the
instrument, or disconnection of the protective
ground terminal may impair the protection
provided by the instrument.
Do not operate the instrument with any covers
or parts removed.
Do not attempt to make adjustments,
replacements or repairs to this instrument
except as described in the accompanying User
Documentation. Only a PerkinElmer service
representative or similarly trained and
authorized person should be permitted to
service the instrument.
Use only fuses with the required current rating
and of the specified type for replacement.
Whenever it is likely that the electrical protec-
tion of the Series 200 UV/VIS Detector has been
impaired, power the Series 200 UV/VIS Detec-
tor off, disconnect the line cord from the elec-
trical outlet, and secure the instrument against
any unauthorized operation.

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Observe the following guidelines for electrical safety:
•
Service on electrical components should be performed only by a qualified PerkinElmer
service representative or similarly trained and authorized person.
•
Always turn off the all components to the Series 200 UV/VIS Detector and disconnect the
power cord from the electrical outlet before you remove any covers.
•
Never operate the Series 200 UV/VIS Detector with any covers or parts removed.
•
Use only fuses with the required current rating and of the specified type for replacement.
Do not use makeshift fuses or short-circuit the fuse holder.
•
The instrument is to be positioned in a clean area free of dust, smoke, vibration, and cor-
rosive fumes, out of direct sunlight, and away from heating or cooling units or ducts.
•
The instrument must be positioned so the power switch on the rear panel can be easily
reached by the operator.
•
There must be an adequate and stable power source for all system components.
This instrument contains a lithium battery
that provides backup power for retaining data
stored internally. There is a danger of battery
explosion should the battery inadvertently be
replaced with an incorrect type or model of
lithium battery. The battery must be replaced
by a PerkinElmer service representative or
similarly trained and authorized person
.
The
expended battery is to be disposed of in
accordance with the battery manufacturer's
recommendations.

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Environmental Considerations
The Series 200 UV/VIS Detector was designed for indoor use and will function correctly
under the following ambient conditions:
Temperature
10 – 35 °C
Relative Humidity
20 – 80% noncondensing
Altitude
0 – 2000 m
Safe operation
5 - 40 °C
Do not operate the Series 200 UV/VIS Detector in
an explosive atmosphere.

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Internal Warning Labels
WARNING
Ultraviolet radiation from this device can
cause eye injury; wear suitable protective
goggles when changing or adjusting the
lamp.
If the detector has been operated recently,
the lamp assembly will be very hot. Check
carefully before touching to avoid personal
injury. Replace lamp with PerkinElmer part
number N292-0149.
NITROGEN
INPUT
3 PSI MAX.
Do not exceed 3 PSI for Nitrogen input
Hot

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Nitrogen Purge
In some cases (for example, in cold rooms or when operating at wavelengths of 200 nm or
lower), it is recommended that you purge the monochromator (via the purge inlet) with puri-
fied nitrogen during use. The flow of nitrogen gas will help reduce or minimize thermal
expansion/contraction of the monochromator, and, in cold rooms, prevent moisture from con-
densing inside the monochromator housing. Use only connecting tubing that is free of soften-
ers or placticizers. Purge with nitrogen at approximately 1-2 psi. (3 psi maximum). Allow 10
to 12 minutes for the monochromator to become completely filled. Continue this nitrogen
flow while the instrument is in use. Purging is particularly important when the instrument
is removed from a cold room and placed in a high-humidity room at ambient temperature.
Detector Flowcell
The standard 12
µ
L flowcell has a normal flow of 1 mL/min of 50/50 ACN/H
2
O. For addi-
tional information, refer to Chapter 7,
Maintenance
for the specific procedures on flowcell
maintenance.
If buffers are being used, flush the flowcell
with water to remove all buffers prior to
maintenance. Buffer may precipitate and
clog the flowcell.
Flowcell backpressure should not exceed 500
psi. Damage to the flowcell and detector
occurs at higher pressures.
Do not overtighten the flowcell connections.
Damage to the flowcell could result.

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Storage Conditions
The Series 200 UV/VIS Detector may be stored under the following conditions:
•
Ambient temperature is –20 to +60 °C
•
Ambient relative humidity is 20 to 80% noncondensing
•
Altitude is in the range 0 to 12 000 m
Dimensions and Weight
The physical dimensions of the Detector are:
Weight:
11.4 kg (25 lbs.)
Height:
16.7 cm (6-9/16 in.)
Width:
33.8 cm (13-5/16 in.)
Depth:
48.6 cm (19-1/8 in.)
Additional Electrical Considerations and Warnings
Installation Category
This instrument is able to withstand transient overvoltage according to Installation Cate-
gory II as defined in IEC 1010-1.
Pollution Degree
This equipment will operate safely in environments that contain nonconductive foreign mat-
ter up to Pollution Degree 2 in IEC 1010-1.
Observe the following guidelines:
•
Service on electrical components should be performed only by a qualified PerkinElmer
service representative or similarly trained and authorized person.
•
Be sure the power cord is the correct one for your laboratory. The line cord used must
meet the national safety agency’s guidelines for the particular country of use.
•
Some power cords available from PerkinElmer are illustrated below:
Do not operate the instrument with any covers
or parts removed.

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Susceptabilit
y to RF Interference
The Series 200 UV/VIS detector complies with the EMC standards for RF emission. How-
ever, it has a small RF susceptibility. An RF field strength of 3 V/M in the frequency range
of 80 to 1000 MHz may cause a 1 x 10
-3
AU deflection on the chromatographic baseline. This
implies that if a device, such as a 5W walkie-talkie carried by a security guard, is used at a
distance of 10 feet from the detector, a spike or peak on the chromatographic baseline of 1 x
10
-3
AU may occur. If you are concerned that such an event may occur, we recommend that
walkie-talkie restriction notices be posted in the laboratory. Cell phones, beepers, and other
similar devices operate in a much higher frequency range and do not cause interference.
Fuses
The following fuses are in the power entry module to protect the instrument:
100, 120, 220, 230/240 VAC at 50/60 Hz: 1.6 Amp, Type T (H - high interrupting capacity), 5
x 20 mm, 250V rated, P/N 0999-1690.
Use the 240V selection on the power entry module for 230/240 VAC nominals.
Note
Service on electrical components should be performed
only by a qualified PerkinElmer service representative
or similarly trained and authorized person The fuses in
the power entry module can be changed by the user.
North America
NEMA-5-15
0998-8986
Europe
CEE 7
"Schuko"
0999-1415
Europe
Switzerland
0999-1413
Europe
Italy
0999-1422
Old British Standard
BS 546
India
0999-1423
British Standard
BS 1363
United Kingdom
0999-1414
Australia
ETSA S/86
0999-1417
Israel
0999-1424
Denmark
0999-1416
Japan
0999-1425

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Quality Control/Good Laboratory Practices
Quality Control
The user should develop appropriate quality control procedures for the Series 200 UV/VIS
Detector to ensure suitability for its intended use. These procedures typically consist of peri-
odic performance verifications and routine inspections and suitability tests.
Certificate of System Control
Each Series 200 UV/VIS Detector is carefully built and tested in a controlled system in
accordance with the requirements specified in its applicable PerkinElmer Final Assembly
and Test Specification.
Each instrument is certified to meet its functional and performance specification upon
release to shipment. The integrity of this quality system is routinely audited and is certified
by the British Standards Institution as meeting all requirements of ISO 9001:1987.
Instrument Performance Verification (IPV)
To maintain functional performance, PerkinElmer recommends a yearly Instrument Perfor-
mance Verification (IPV) of the Series 200 UV/VIS Detector and its other system compo-
nents by a PerkinElmer Service Engineer to ensure its operation within published
specifications. These tests consist of measuring the most important UV/VIS characteristics
such as noise drift and wavelength accuracy. Certification is available for regulatory compli-
ance. Contact your local PerkinElmer Sales and Service office.
Routine Inspection and
Suitability Test
Prior to any sample analysis, a system suitability test, which closely resembles the intended
assay, should be performed to ensure that the LC system is operating within established cri-
teria (e.g., peak resolution, peak asymmetry, precision, retention time, column plate count,
pressure limits, signal/noise ratio, etc.)
While the Universal Test Mix (UTM) P/N 0089-0893 can be used for a system check, we rec-
ommend that you develop a separate system suitability test and acceptance criteria for each
of your assays.
Hazardous Chemicals
Before using mobile phase solvents, you should be thoroughly familiar with all hazards and
safe handling practices. Observe the manufacturer’s recommendations for use, storage and
disposal. These recommendations are normally provided in the material safety data sheets
(MSDS) supplied with the solvents.

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Definitions in Warning for Hazardous Chemicals
Some chemicals used with this instrument
may be hazardous or may become hazardous
after completion of an analysis. The responsi-
ble body (e.g., Lab Manager) must take the
necessary precautions to ensure that the sur-
rounding workplace and instrument opera-
tors are not exposed to hazardous levels of
toxic substances (chemical or biological) as
defined in the applicable Material Safety
Data Sheets (MSDS) or OSHA, ACGIH, or
COSHH documents. Venting for fumes and
disposal of waste must be in accordance with
all national, state and local health and safety
regulations and laws.
Responsible body:
“Individual or group responsible for the use and maintenance of equip-
ment, and for ensuring that operators are adequately trained.” [per IEC
1010-1, Amendment 2]
Operator:
“Person operating equipment for its intended purpose.” [per IEC 1010-1,
Amendment 2]
OSHA:
Occupational Safety and Health Administration (United States)
ACGIH:
American Conference of Governmental Industrial Hygienists
COSHH:
Control of Substances Hazardous to Health (United Kingdom)

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Series 200 Operating Precautions
To protect yourself from harm and to prevent malfunctioning of the system, please observe
the following guidelines when operating the UV/VIS Detector:
•
Before using the instrument, pay attention to the warnings presented in the beginning of
the manual.
•
Wear the appropriate protective clothing, including safety glasses and gloves, when pre-
paring samples and solutions for use with this instrument.
•
Always follow the correct safety procedure and the manufacturer's recommendations
when using solvents.
•
Keep solvents with a boiling point less than 30
°
C under helium (10 kPa) in an optional
solvent cabinet (for example, the SEC 4).
•
Do not keep buffer solutions in the system when the instrument is not in use, since salt
may crystallize out.
•
Do not use acidic chlorides with pH less than 7; they can cause corrosion. Chloroform and
tetrachloromethane decompose slowly and form HCl.
•
Do not use chloride TRIZMA buffers since they form HCl in equilibrium. Use phosphate
buffers instead.
•
Do not use inorganic acids, concentrated organic acids or acidic buffer solutions.
•
Ensure that the system is flushed with degassed solvent.
•
Ensure that the other instruments in the system are ready to run.
•
Ensure that all connecting communications and power cables are correctly installed for
your analysis.
•
It is possible to clean the exterior surfaces of the instrument with a soft cloth dampened
with a mild detergent and water solution. Do not use abrasive cleaners or solvents.

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Solvents
•
Always use clean solvents. Solvents which have been distilled in glass (HPLC Grade) are
recommended.
•
Filter the solvents and buffers through a 0.5-micron medium as an additional precau-
tion.
•
Degas all aqueous and most organic solvents prior to use.
•
Store flammable solvents or solvents which may form hazardous by-products when the
instrument is shut down, by following the recommended shutdown procedure.
•
Check compatibility of solvent(s) with the type of column(s) being used.
•
Know the relative polarity and miscibility of the solvents being used.
Solvents with Low Boiling Points
Do not use liquids that have a boiling point less than 30 °C.
Buffers
Exercise care when using buffers in conjunction with organic solvents. NEVER LEAVE
BUFFERS IN THE SYSTEM OVERNIGHT. Buffers left in the system can form salt crystals
which may cause premature injector valve failure and plug the transfer tubing and sample
needle. To remove buffers, flush the system with water followed by methanol or isopropanol.
Remember to change the flush solvent from methanol or isopropanol to water before using
buffers.
Do not use carbon disulfide or other solvents
which have an auto-ignition temperature
below 110 °C.

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Corrosion
The following corrosion precautions apply to the standard (stainless steel) UV/VIS and the
biocompatible (titanium) UV/VIS Detectors.
Standard
All parts of the Series 200 UV/VIS Detector that contact mobile phase are made of stainless
steel, KelF, and quartz. Some of these materials are extremely sensitive to acid chlorides. If
you have questions about your mobile phase or flush solvent, contact a PerkinElmer repre-
sentative. Refer to the table below.
Table S-1.
Solvents Which May Corrode the Series 200.
Biocompatible
All parts of the Series 200 UV/VIS Detector that contact the mobile phase (from the pump)
and flush solvent are made of titanium, fluoropolymers, or glass. This makes the Series 200
UV/VIS Detector inert to the most common mobile phases that are used for biological assays.
If you have questions about your mobile phase, contact a PerkinElmer representative. Refer
to the table below.
Table S-2.
Solvents Which May Corrode the Series 200.
Aqua Regia (80% HCl, 20% HNO3)
Hydrogen Peroxide
Anhydrous Chloride
Dichloromethane
Sulfuric Acid (Conc.)
Hydrochloric Acid (20% and 37%)
Hydrofluoric Acid (20%, 50%, and 75%)
Copper Chloride
Bromine
Freon 12 (wet)
Chlorinated solvents
Hydrofluorosilicic Acid (20%)
Hydrobromic Acid (20%)
Ferric Chloride
Ferrous Chloride
Mercuric Chloride (Dilute)
Aqua Regia and sulfuric acid are known to
dissolve titanium.
Aqua Regia (80% HCl, 20% HNO3)
Hydrogen Peroxide
Dichloromethane
Anhydrous Methanol
Chlorinated solvents
Sulfuric Acid

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Solvents with Auto-Ignition Temperature Below 110 °C
Certain solvents have a temperature at which they are combustible upon contact with a
heated surface, even in the absence of a spark or other source of ignition. A list of common
solvents with their auto-ignition temperatures are in Table S-3.
Table S-3.
Auto-Ignition Temperatures of Common LC Solvents.
Do not use carbon disulfide or other solvents
which have an auto-ignition temperature
below 110 °C.
Solvent
Auto-Ignition
Temperature (°C)
Carbon Disulfide
100
Diethyl Ether
180
Cyclohexane
260
Hexane
261
Petroleum Ether (naphtha)
288
Pentane
309
Tetrahydrofuran
321
Dioxane
366
Propanol
404
iso-Octane
418
Ethanol
423
Isopropyl Ether
443
Dimethylformamide
445
Methanol
446
Isopropanol
455
Methylethylketone
474
Acetonitrile
524
Ethyl Acetate
524
Toluene
536
Acetone
538
Benzene
562