Q-sense E1 Manual

©Q-SENSE AB 2007
Q-SENSE E1 LIQUID HANDLING SET UP
Q-Sense E1 Pump and Flow System Setup
Part 1 – Ismatec Reglo Analog
Before switching the pump
on, check the voltage
setting and fuse on the
backside:
Dec 2007, Rev A
220-240V: use 250mA fuses
110-120V: use 500mA fuses
1
Insert the 3-stop color-coded tubing into the cassettes.
Voltage settings
window
2
Set the pump speed by
adjusting the 2-digit selector.
The pump tubing supplied by
Q-Sense has an ID of 0.38
mm, giving the following flow
rates:
3.2 rpm = 28 µl/min (select “01”)
160 rpm = 1.4 ml/min (select “99”)
For details and calibration function, see supplier’s manual.
Part 2 – Tubing connectors
The ferrule forms a tight
hen being screwed into
e
ed
d in
dicate the direction of
le
on.
seal as it is deformed
w
the flow module. Place th
ferrule and threaded
connector as shown
before attaching them.
Make sure to cut the
PTFE tubing at right
angles. It is recommend
to use the blue tube
cutting device include
the Liquid Handling Set.
Note that the arrows on
the top of the module
in
the flow for correct samp
temperature equilibrati
3
4
Out
In

Dec 2007, Rev A
©Q-SENSE AB 2007
Q-SENSE E1 LIQUID HANDLING SET UP
Use the brown adapter
(the perifit) to connect the
PTFE tubing and black
threaded nut to the soft
pump tubing (expanding
the narrow bore of the
pump tubing with a pipet
tip may help). If 1.5-2mL
tubes are used, the whi
lids can be used to guide
the tubing.
te
te
Note that the
lid needs to be vented to
work properly.
Place the pump
and
ample holder so that the
amples are drawn
ule.
mp
or details, please chec
s
s
through the flow mod
This setup will reduce the
need to clean the pu
tubing and prevents
leakage.
5
F k the E1 Operator Manual.
6