RI SATURN 5 User manual

User Manual
SATURN 5 LASER SYSTEMS
™
only
0120
0120
[phone] | customer[email protected] | coopersurgical.com
Research Instruments Ltd, Bickland Industrial Park, Falmouth, Cornwall TR11 4TA, UK
Document 6-47-500UM(15) | DRF 4915 | 25th June 2019

CONTENTS
 
 
Intended Use 5
Indicaon for Use - USA Customers Only 5
Applicable Part Numbers 5
Microscope Compability 5
Installaon 6
 
Cauons and Warnings 7
Precauons 9
Guidance and Manufacturer’s Declaraon – Electromagnec Emissions (IEC 60601-1-2) 9
Guidance and Manufacturer’s Declaraon — Electromagnec Immunity 10
Safety and Informaon Symbols 12
 
System Components 13
Saturn 5 Laser System Component Part Numbers 14
Saturn 5 Acve Laser System Mirror Module Part Numbers 14
Saturn 5 Laser System Collimator Module Part Numbers 15
Hardware Overview 15
Laser Specicaons 16
Objecve Specicaons 16
Saturn Acve Specicaons 16
Electrical Specicaons 16
Mains Adaptor for Control Unit and Motor Module 16
Dimensions 16
Operang Condions 16
Storage/Transport Condions 16
 
Introducon 17
User Interface Icons 17
How to Zoom & Pan the Image 18
There are three ways to zoom into an area on the screen. 18
How to Set the Preset Zoom 18
How to Select Cameras 18
Camera Flipping 18
Changing Camera Sengs 19
Objecves 19
How to Select Objecves 19
How to Add Objecves 19

How to Remove Objecves 19
How to Check Objecve Calibraon 19
How to Congure the Foot Pedal/Keyboard 20
How to Perform Measurements 21
How to Take a Picture 21
How to Record Video 21
RI Viewer Gallery 22
 
Laser Assisted Hatching 28
Embryo Biopsy Procedure 28
Cleavage Stage Blastomere Biopsy 28
Trophectoderm/Blastocyst Biopsy 29
Blastocyst Collapse 29
 
Hole Size Selecon 31
Ablaon Procedure 31
 
How to Start Up 33
How to Congure the Laser for a New Procedure 33
How to Check Laser Target Alignment for Saturn 5 Acve Laser System 33
How to Check Laser Target Alignment for Saturn 5 Fixed Laser System 34
How to Check Hole Size Calibraon 34
How to Remove a Point from the Hole Size Calibraon Graph 35
Laser Target 35
Hole Size Indicator 35
Exclusion Zone 35
How to Adjust Hole Size/Pulse Width 36
Preset Pulse Widths 36
How to Fire the Laser (Single Pulse) 36
Biopsy Mode 37
How to Enable Biopsy Mode 37
How to Fire the Laser (Biopsy Mode) 37
How to Shut Down 37
 
 
Cleaning 39
Servicing 39
Disposal of Goods 39
 
 
Obligaon to Inform 42
Feedback 42

Secon 1
Preface
1
4
Research Instruments Ltd

Thank you for choosing the Saturn 5 Laser System.
This manual provides all necessary informaon to use the Research Instruments Ltd Saturn 5 Laser
System. The system should be operated by trained personnel only. All secons of this manual should
be read and understood fully before any operaon of the system. Please see the Intended Use for more
informaon.
If the operator is unsure of any of the informaon contained in this manual, they should contact
Research Instruments or an appointed representave before aempng to use this equipment.
In no event does Research Instruments Ltd (RI) assume the liability for any technical or editorial errors
of commission, or omission; nor is Research Instruments Ltd liable for direct, indirect, incidental, or
consequenal damages arising out of the use or inability to use this manual.
© This manual is protected by copyright, all rights reserved, and no part hereof may be photocopied or
reproduced in any form without the prior wrien consent of Research Instruments Ltd.
Saturn™ and RI Viewer™ are trademarks of Research instruments Ltd. All other brand names are
trademarks of their respecve owners.
The informaon in this manual is current at the me of publicaon. RI is constantly updang its
products, and, therefore, reserves the right to introduce changes in design, equipment and technical
features at any me. The latest version of the User Manual as well as RI Viewer soware can be
downloaded from soware.research-instruments.com.
The Saturn 5 Laser System manual belongs with the laser and should be passed on with the Saturn 5
Laser System if relocated to another clinic.

Secon 1
1
Preface

Research Instruments Ltd
6-47-500 - Saturn 5 Acve Laser System
6-47-501 - Saturn 5 Fixed Laser System

Zeiss Axiovert 40/100/200/Observer
Nikon TMD, D200/300, TE200/300, TE2000, Ti, Ti2
Leica DMIRB, DMI3000B/4000B/6000B, DMIL, DMi8
Olympus IMT2, IX50/70, IX51/71/81, IX53/73/83
Applicable indicaons for use are subject to the regulaons of the country into which the device is sold.
only
0120
0120


Intended Use

For use in assisted reproducon procedures to ablate or thin the zona pellucida of an embryo to
facilitate assisted hatching or recovery of cells for pre-implantaon genec diagnosis (blastomeres).
The device can also be used on blastocyst stage embryos for biopsy of trophectoderm cells for pre-
implantaon diagnosis procedures, and blastocyst collapse prior to vitricaon procedures.
 Federal law restricts this device to sale by or on the order of a physician or a praconer
trained and cered in its use
The Saturn Laser System is intended to ablate a User defined section of a gamete or embryo
within ART.

Secon 2
Introducon to Saturn 5™ Laser Systems
2
6
Research Instruments Ltd

The PC is an intrinsic part of the Saturn 5 Laser System. It may be connected to a Local Area Network
(LAN) only. Only install RI Viewer soware. We recommend PC security features such as an-virus
soware/rewall be installed consistent with the facility’s IT policy and that each user of the system
has a password protected user prole assigned to the PC in order to prevent unauthorised access.
Installaon of the Saturn 5 Laser System should be carried out by a Research Instruments technician
or other RI-authorised personnel. Incorrect installaon could result in reduced power output, laser
misalignment or laser malfuncon. Relocaon of this system should be treated as a re-installaon and
should, therefore, be carried out by authorised personnel.
Please note that installaon and servicing is covered separately in the Saturn 5 Laser System Installaon
Manual (6-47-500IM), which is available to service providers.
A Patients Guide to Laser Assisted Hatching (LAH), Laser Assisted Biopsy (LAB) and Blastocyst
Collapse are provided on the RI Viewer software. RI Viewer software can be downloaded from
hp://soware.research-instruments.com
Once installed, the RI Viewer applicaon can be started by double clicking the RI Viewer icon on the
desktop.

Secon 3
3
Saturn™

Research Instruments Ltd


Laser radiaon is present within the control unit, the bre opc patch lead and through the micro-
scope to output at the objecve. The Saturn 5 Laser System has been designed to ensure that emis-
sion of laser radiaon at any point is within the limits spulated by internaonal safety standards,
IEC 60825-1:2014, and US 21CFR1040.10 for unprotected viewing (Class 1). As an extra precauon, an
infrared blocking lter has been incorporated into the opcal path leading to the eyepieces to reduce
laser emission by a further 99%.
For a descripon of controls and for the locaon of laser aperture on control unit, see Secon 4 -
Hardware Overview.
 disassemble or modify any part of the Saturn 5 Laser System, or substute any
component for any other. Doing so may result in exposure to dangerous levels of laser
radiaon. This voids the warranty and/or service contract.
 operate the laser unless it is properly mounted to a microscope.
 use the power cable and power supply adaptor supplied with the system.
The cable to the power supply is the Disconnect Device for this equipment. To remove all
electrical power from this product, disconnect the power cable from the electrical outlet.
Equipment should be posioned so as to allow easy access to the power cable. The appliance
coupler or mains plug is used as the disconnect and must remain readily operable.
 To avoid the risk of electric shock, this equipment must only be connected to
a mains supply with protecve earth.
 Not to be used in a paent environment
CAUTION Hazardous radiaon exposure may occur through the use of controls, execung
adjustments or the performance of procedures other than those specied herein. Servicing
and adjustment other than that specied in this manual should only be carried out by RI
or RI authorised agents.
This indicates cauonary text which should be followed to avoid injury to users or damage
to samples.
The system should be operated by qualied and trained personnel only.

Secon 3
Saturn™ 5 Laser Systems Safety Warnings
3

Research Instruments Ltd
The embryo may be damaged if objecves other than the red Saturn 5 Laser
System objecve are used.
Do not modify this equipment without authorisaon of the manufacturer.
Where an ITO glass heated stage is ed, do not re the laser with the
microscope focussed into the ITO glass surface as this may damage the ITO coang.
Thermal lensing is a defocusing of the laser beam caused by changes to
the refracve index of the medium as it is heated. The degree of defocus depends on
the total beam energy deposited in the medium. While slight defocus has no eect on
the drilling properes, this eect may become a problem for higher beam energies. To
eliminate any signicant eects from thermal lensing, we recommend that the pulse
width be kept below 1000μs for clinical Laser Assisted Hatching (LAH) zona drilling.
Only a single opening should be made in the zona pellucida. Mulple openings
or those that are too small may prevent embryo hatching or lead to abnormal development.
Do not operate the pilot laser with an embryo in the eld of view. Exposure
to the pilot laser may damage the embryo.
The microscope itself must be maintained to a high standard. Problems such
as worn focus mechanisms or an insecure video camera may lead to unreliable focus and
image stability, and could lead to embryo damage.
Before performing any procedure, the pilot laser must be used to check
the alignment of the Acve posioning system. The system must be re-aligned when
necessary. See Secon 8 How to Check Laser Target Alignment.

Secon 3
3
Saturn™
9
Research Instruments Ltd
Emissions test  

RF emissions
CISPR 11
Group 1
The Saturn 5 Laser System uses
RF energy only for its internal
funcon. Therefore, its RF
emissions are very low and are
not likely to cause
any interference in nearby
electronic equipment.
RF emissions
CISPR 11
Class B
The Saturn 5 Laser System
is suitable for use in all
establishments, including
domesc establishments and
those directly connected to
the public low-voltage power
supply network that supplies
buildings used for domesc
purposes.
Harmonic emissions
IEC 61000-3-2
Class A
Voltage uctuaons/icker
emissions Complies

The Saturn 5 Laser System is intended for use in the electromagnec environment specied below. The
customer or the user of the Saturn 5 Laser System should assure that it is used in such an environment.
*Kanyo, K., Konc, J. “A follow-up study of children born aer diode laser assisted hatching.” European
Journal of Obstetrics and Gynaecology. 110: 176-180 (2003).

• To minimise the risk of damage to blastomeres, administer as few laser pulses as possible at the
lowest energy levels possible to achieve the prescribed zona drilling or thinning eect.
• Direct the laser beam toward a secon of the zona pellucida where the adjacent perivitelline space
is widest or next to an area of fragmentaon.
• A holding pipee should be used during laser treatment to minimise the risk of embryo movement.
• Small openings in the zona pellucida may lead to embryo constricon and abnormal development.
• To date there are no known reports showing a greater occurrence rate of major or minor defects
in children derived from laser-hatched embryos. Long-term follow-up data on children born from
laser-hatched embryos does not yet exist. A study of 134 such babies* found no increase in the major
congenital malformaons, chromosomal aberraons or minor congenital malformaons between
the LAH treated group and all deliveries at their hospital.
• The device is not aected by and does not present any reciprocal interference to the microscope.
• During normal use there is no contact with paent/sample.

Secon 3
Saturn™ 5 Laser Systems Safety Warnings
3

Research Instruments Ltd

Compliance with the emissions requirements of CISPR 22 Class A requires the following warning:
“This is a class A product. In a domesc environment this product may cause radio interference in
which case the user may be required to take adequate measures.”

The Saturn 5 Laser System is intended for use in the electromagnec environment specied below. The
customer or the user of the Saturn 5 Laser System should ensure that it is used in such an environment.
 

 

Electrostac
discharge (ESD)
IEC 61000-4-2
± 6 kV contact
± 8 kV air
± 6 kV contact
± 8 kV air
Floors should be wood, concrete
or ceramic le. If oors are
covered with synthec material,
the relave humidity should be at
least 30 %.
Electrical fast
transient/burst
IEC 61000-4-4
± 2 kV for power
supply lines
± 1 kV for input/
output lines
± 2 kV for power
supply lines
± 1 kV for input/
output Lines
Mains power quality should be
that of a typical commercial or
hospital environment.
Surge
IEC 61000-4-5
± 1 kV line(s) to
line(s)
± 2 kV line(s) to earth
± 1 kV dierenal
Mode
± 2 kV common
mode
Mains power quality should be
that of a typical commercial or
hospital environment.
Voltage
dips, short
interrupons and
voltage variaons
on power supply
input lines
IEC 61000-4-11
<5% UT
(>95% dip in UT ) for
0.5 cycle
40% UT
(60% dip in UT) for 5
cycles
70% UT
(30% dip in UT) for 25
cycles
<5 % UT
(>95 % dip in UT) for
5s
<5 % UT
(>95 % dip in UT) for
0.5 cycle
40 % UT
(60 % dip in UT) for
5 cycles
70 % UT
(30 % dip in UT) for
25 cycles
<5 % UT
(>95 % dip in UT)
for 5s
Mains power quality should be
that of a typical commercial
or hospital environment. If
the user of the Saturn 5 Laser
System requires connued
operaon during power mains
interrupons, it is recommended
that the Saturn 5 Laser System be
powered from an uninterrupble
power supply or a baery.
Power frequency
(50/60 Hz)
magnec eld
IEC 61000-4-8
3 A/m 0.3 A/m The power frequency magnec
eld should be measured in the
intended installaon locaon to
assure that it is suciently low.
UT is the a.c. mains voltage prior to applicaon of the test level.

Secon 3
3
Saturn™
11
Research Instruments Ltd

test





Conducted RF
IEC 61000-4-6
Radiated RF
IEC 61000-4-3
3 Vrms
150 kHz to 80 MHz
3 V/m
80 MHz to 2.5 GHz
3 Vrms
3 V/m
Portable and mobile RF communicaons
equipment should be used no closer to any part
of the Saturn 5 Laser System including cables,
than the recommended separaon distance
calculated from the equaon applicable to the
frequency of the transmier.

d = 1,2√P
d = 1,2√P 80 MHz to 800 MHz
d = 2,3√P 800 MHz to 2 , 5 GHz where P
is the maximum output power rang of
the transmier in was (W) according to
the transmier manufacturer and d is the
recommended separaon distance in metres
(m).
Field strengths from xed RF transmiers, as
determined by an electromagnec site survey,
a should be less than the compliance level in
each frequency range. b
Interference may occur in the vicinity of
equipment marked with the following symbol:
 At 80 MHz and 800 MHz, the higher frequency range applies.
 These guidelines may not apply in all situaons. Electromagnec propagaon is aected by
absorpon and reecon from structures, objects and people.
a Field strengths from xed transmiers, such as base staons for radio (cellular/cordless) telephone
and land mobile radios, amateur radio, AM and FM radio broadcast and TV broadcast cannot be predicted
theorecally with accuracy. To assess the electromagnec environment due to xed RF transmiers,
an electromagnec site survey should be considered. If the measured eld strength in the locaon in
which the Saturn 5 Laser System is used exceeds the applicable RF compliance level above, the Saturn
5 Laser System should be observed to verify normal operaon. If abnormal performance is observed,
addional measures may be necessary, such as re-orienng or relocang the Saturn 5 Laser System.
b Over the frequency range 150 kHz to 80 MHz, eld strengths should be less than 3 V/m.


Secon 3
Saturn™ 5 Laser Systems Safety Warnings
3
12
Research Instruments Ltd

 
In accordance with Annex II of the European Medical Device Direcve 93/42/EEC,
as amended by Direcve 2007/47/EC under the supervision of noed body SGS
Cauon: US Federal law restricts this device for sale to or on the order of a
licensed healthcare praconer
Indicates the need for the user to consult the instrucons for use for
important cauonary informaon such as warnings and precauons that
cannot, for a variety of reasons, be presented on the medical device itself
Manufacturer
Date of Manufacture
Reference number
Serial Number
Refer to Instrucon manual
Consult instrucons for use
Direct current (DC)
Class 1 laser product (IEC60825-1:2014)
Do not dispose of product with normal waste
Disposal of according to EU WEEE Direcve
This way up
Fragile, handle with care
Stacking limited to 3 units
Keep dry
0120
0120
Only
CLASS 1

3
Source: BS EN ISO 15223-1:2016
BS EN 60601-1:2006+A12:2014

Secon 4
4
13
Saturn™5 Laser Systems Product Overview
Research Instruments Ltd

Welcome to the User Manual for the Research Instruments Saturn 5 Laser System. This manual covers
the set-up, operaon and maintenance of the system.
This device is for use in assisted reproducon procedures to ablate or thin the zona pellucida of an embryo
to facilitate assisted hatching or recovery of cells for pre-implantaon genec diagnosis (blastomeres).
The device can also be used on blastocyst stage embryos for the biopsy of trophectoderm cells for pre-
implantaon diagnosis procedures, and blastocyst collapse prior to vitricaon procedures.
The system is used with an inverted microscope, PC and camera to provide an opcal system and means
of ring a laser. The system cannot be used without a PC or RI Viewer soware.
The Saturn 5 Laser System is oered in two opons, Fixed or Acve models. The Acve model allows
the user to move the posion of the laser to the target area on the sample. The Fixed model has the
laser in a xed posion and the user moves the sample to the target.
All laser movement (Acve model only) and laser ring is user controlled.

Note: Actual components may dier from those shown.
The Saturn 5 Laser System comes supplied with the following parts: a control unit containing the lasers,
a mirror module, armoured patch lead and a special objecve. If you have a Saturn 5 Acve Laser System
then an addional motor module will be supplied. The laser beams are delivered to the specimen through
a bre opc patch lead, collimator module (that is part of the mirror module for a Fixed system or the
motor module for an Acve system) and the special objecve.
A foot pedal for ring the laser is available as an oponal extra. This is aached to the PC by a USB cable.
The motor module receives coordinates via the USB which moves the motors to those coordinates. The
control unit then receives commands from the soware to re for a specic length of me.
Mirror Module
Power Supply
Motor Module
Patch Lead
Objecve
Control Unit

Secon 4
Saturn™ 5 Laser Systems Product Overview
4
14
Research Instruments Ltd
The following tables give part numbers of system components and microscope adaptors. All parts are
supplied or manufactured by Research Instruments Ltd.
 
5-47-010 Control Unit with 1.5m Patch Lead
5-47-100 Motor Module (Saturn 5 Acve Laser System)
1-10-133 12V 3.3A Medical PSU (x2 for Saturn 5 Acve Laser System)
5-45-500 Laser Objecve (red) 40x
5-45-507 Laser Objecve (red) 20x*

 
5-46-163 Mirror module for Olympus IX53/73/83 le
5-46-150 Mirror module for Olympus IX50/70 le
5-46-160 Mirror module for Olympus IX50/70 right
5-46-151 Mirror module for Olympus IX51/71/81 le
5-46-152 Mirror module for Olympus IX51/71/81 right
5-46-159 Mirror module for IMT2
5-46-135 Mirror module for TMD
5-46-132 Mirror module for Nikon Diaphot 200/300
5-46-130 Mirror module for TE 200/300
5-46-140 Mirror module for TE2000 with Integra Ti
5-46-141 Mirror module for TE2000 without Integra Ti
5-46-142 Mirror module for Nikon Ti with Integra Ti
5-46-144 Mirror module for Nikon Ti with X-Y
5-46-146 Mirror module for Nikon Ti2 with Integra
5-46-148 Mirror module for Nikon Ti2 with XY stage
5-46-158 Mirror module for Axiovert 40
5-46-110 Mirror module for Axiovert 200/Observer
5-46-120 Mirror module for DMIRB
5-46-122 Mirror module for DMI3000B
5-46-121 Mirror module for DMI4000B/6000B
5-46-124 Mirror module for DMi8

*Not cleared for use in USA market.

Secon 4
4

Saturn™5 Laser Systems Product Overview
Research Instruments Ltd
5-45-163 Collimator module for Olympus IX53/73/83
5-45-383 Collimator module for Olympus IX50/70
5-45-387 Collimator module for Olympus IX51/71/81
5-45-388 Collimator module for Olympus IX71/71/81 uor
5-45-580 Collimator module for IMT2
5-45-411 Collimator module for TMD
5-45-301 Collimator module for Nikon Diaphot 300/TE300
5-45-545 Collimator module for TE2000/Nikon Ti
5-45-546 Collimator module for TE2000/Nikon Ti uor
5-45-575 Collimator module for Axiovert 40
5-45-009 Collimator module for Axiovert 100
5-45-011 Collimator module for Axiovert 200/Observer
5-45-450 Collimator module for DMIRB
5-45-470 Collimator module for DMIL
5-45-461 Collimator module for DMI3000B
5-45-460 Collimator module for DMI4000B/6000B
5-45-463 Collimator module for DMi8



CLASS 1

LASER OUTPUT
KEEP CLEAN

PILOT m


Research Instruments Ltd, Bickland Industrial Park,
Falmouth, Cornwall, TR11 4TA, UK
surm

CS

FOLL
INSTRUCTIONS
FOR USE
UDI
1
11
21
PC 
USE AAPTOR

A

Secon 4
Saturn™ 5 Laser Systems Product Overview
4
16
Research Instruments Ltd
Control unit 220mmx180mmx34mm (8.6”x7.1”x1.3”)
 1.2Kg


  Pilot Laser
Output wavelength 1480nm 650nm
Power output from patch lead 400mW 180µW (max)
Maximum pulse width 2.0ms None
Laser Safety Classicaon* Class 1 Class 1
* IEC 60825-1:2014, US 21CFR 1040.10


 100-240VAC, 50-60Hz, 1.0 - 0.5A
 40W
 12VDC
 3.3A
 Mandatory 3-pin plug for earthing (grounding)
 Approx. 370μm (40x), 740μm (20x)
 1μm within calibraon area, 3μm outside (see page 33)

Manufacturer Actual Magnicaon
Olympus 40x, 20x
Nikon (CFI60) 36x, 18x
Leica 36x, 18x
Zeiss 44x, 22x
  
 0.49 0.25
 2.5mm 2.5mm
Parfocal distance 45mm (adaptor supplied for Nikon CFI60 systems)

* Not cleared for use in USA market.
40x or 20x is a nominal gure. Actual magnicaon will vary slightly when ed to dierent microscopes.
 10°C (50°F) to 42°C (108°F)
 15% to 85% RH (Non-condensing)
Pressure Pressure Range: 70kPa. to 108kPa

 -40°C (40°F) to 60°C (140°F)
 15% to 85% RH (Non-condensing)
Pressure Pressure Range: 70kPa. to 108kPa.


Secon 4
4

Saturn™5 Laser Systems Product Overview
Research Instruments Ltd

Icon  Icon 
Take Picture Laser Control
Take Video General Sengs
Stop Video
Recording
Addional Camera
Sengs
Open RI Viewer
Gallery
Take Measurements
Choose Objecve
Magnicaon Display Rulers Tool
Increase/Decrease
Zoom Level Instrucons For Use
User Interface Icons
RI Viewer is the soware that interacts with the Saturn 5 Laser System for use within an ART laboratory.
Connected to a camera aached to a microscope, it provides on-screen images of samples under the
microscope. It can record video and store sll images from the microscope in a PC’s le system. End
users should ensure their own PCs are cered compliant to IEC 60950-1.
The operator should be posioned to easily access the microscope and also view the image on the PC
monitor.
RI Viewer soware can be downloaded from hp://soware.research-instruments.com
Once installed, the RI Viewer applicaon can be started by double clicking the RI Viewer icon on the
desktop.


Secon 5
RI Viewer ™
5

Research Instruments Ltd
There are three ways to zoom into an area on the screen.
1. Place the mouse at a point on the screen and click and hold the right mouse buon then release.
This will zoom into the spot where the mouse is placed. To zoom out, click and hold the right mouse
buon.
2. Place the mouse at a point on the screen and use the scroll wheel on the mouse (if available).This
will give the user control over how much digital zoom is given.
3. Using the tool bar at the boom of the screen, click the + icon to zoom in incrementally. To zoom
out by the same amount, click on the  icon on the tool bar. The magnifying glass icon will zoom in
and out by the preset zoom.
Whilst using digital zoom, a thumbnail image of the screen will appear in the top le of the screen. At
the top of this panel is the amount of magnicaon you are using. When zoomed out fully, this panel
will not appear on the screen.
Zooming in and zooming out can also be achieved using the foot pedal. (see page 20)

1. Click the  icon on the tool bar.
2. Drag the  slider to the required zoom.

1. Click the  icon on the tool bar. The Video Source drop-down box will have a list of
camera names referring to the cameras connected to the PC.
2. Click on the drop-down box to allow the selecon of cameras. Clicking on the required camera in
the list will change the live image to that of the selected camera.
 The list of available cameras is automacally populated to show the aached devices. Camera
names in this list cannot be altered by the user.

1. Click the  icon on the tool bar.
2. Tick(for camera devices that support these opons).
3. Tick  (for camera devices that support these opons).
Camera ipping is for microscopes where the camera image may be required to be inverted. This is
carried out during installaon where a camera port requires camera ipping to show the image correctly
on the screen. This will be carried out by a trained RI Service Engineer or company representave. When
camera ipping is required it is recommended to carry out Laser Target Alignment as per instrucons
before carrying out any procedure.


Secon 5
5

19
Research Instruments Ltd

Clicking the  icon on the tool bar will show a panel indicang the camera
name and frames per second (fps). Clicking on  will allow the user to adjust sengs
on the video source. Only sengs available to the video source chosen will appear.

Objecves can be changed on the microscope to enable the user to get a beer eld of view or beer
magnicaon. If you require accurate measurements of the image, the objecve chosen in RI Viewer
must be the same magnicaon as the objecve in use on the microscope.

1. Click the icon on the tool bar.
2. Click the required objecve magnicaon.

1. Click  icon on the tool bar.
2. Click the  buon.
3. Click . The magnicaon will be the same as the current magnicaon.
4. Click the required objecve to edit Objecve Magnicaon and Fine Adjustment values.

1. Click  icon on the tool bar.
2. Click the  buon.
3. Click  next to the objecve to be removed.

1. Click the  icon on the tool bar.

Secon 5
RI Viewer ™
5

Research Instruments Ltd
2. Click the  buon to open the
Objecve Calibraon panel.

1. Click the  icon on the tool bar.
2. From the Sengs panel click the .
3. Click the corresponding edit buon to assign a shortcut.
4. Hold down the key or press the foot pedal to assign.
5. Click .
Once the shortcuts are set up, press the shortcut keys or the corresponding foot pedal to acvate the
command.
3. Place an object of known dimensions (a stage micrometer
is supplied with each system for this purpose) in the eld of
view.
Ensure that the objecve selected in RI Viewer matches
that being used on the microscope.
4. The stage micrometer supplied measures 100µm between
the longer lines. For best accuracy, posion each end point
of the line at exactly the same relave posion on the
scale, for example at the right hand edge of each vercal
line.
5. Drag the rulers to the point where the stage micrometer
is going to be measured from and use the ne adjustment
up and down unl the ruler scale matches the stage
micrometer.