RS Mini Galaxy E 050-600 User manual

CO
2
Incubator
Model No: 050-600
User Manual

1
Issue E, January 2009
Contents
ContentsContents
Contents
Section Page No
1. Product Description 2
2. Guidance Notes and Routine Checks 3
3. Installation 4
4. Setting the Hu idity Conditions 7
5. Control Panel Description 8
6. Progra ing Te perature, CO2 & Auto Zeroing 9
7. Progra ing the Alar Syste 10
8. The Alar Syste 11
9. Syste Alar s 13
10. Cleaning and Disinfecting 14
11. Technical Specification 15
12. Ordering Infor ation 16
13. Accessories 18
If this incubator is fitted with Model Options, User Instructions and infor ation is located at the back,
after Ordering Infor ation.

2
Section 1
Section 1Section 1
Section 1
Product Description
The MiniGala y E CO
2
Incubator is designed to ensure accurate and reliable operation. The unit
incorporates a touch sensitive keypad and two, 3 digit L. E. D. displays which allows easy setting and
onitoring of the cha ber condition.
The icroprocessor control syste ensures a rapid, controlled return to opti u cha ber conditions
after a door opening whilst also preventing any overshoot.
A ther al heating ele ent co pletely surrounds the incubator providing an even te perature within
the cha ber. The independently heated outer door is designed to ensure an even distribution of heat
thereby eli inating condensation on the inner door.
Incorporated into the unit is an over te perature safety syste . This operates independently of the
ain control syste .
A purpose designed hu idity control syste allows a high unifor relative hu idity and prevents
undesirable condensation in other parts of the cha ber. The anual control syste allows li ited
adjust ent over an approxi ate range of 95 – 97% r/h.
A solid state infra red sensor is used to control the level of CO
2
giving excellent reliability and is
unaffected by hu idity. The CO
2
syste has a se i-auto atic zero function which provides a si ple
process for the user to follow to aintain the correct CO
2
level within the cha ber.
There is a two level alar syste . The cha ber onitoring alar is progra able and will alert you
if te perature or CO
2
have not recovered within a preset ti e after the door has been opened. If not
required, this syste can be disar ed. Syste alar s occur only if a fault has developed which
requires user intervention to rectify.
Air circulation is achieved without the use of a fan eli inating this potential source of conta ination
and reducing s all sa ple evaporation within the cha ber.
The 50 litre cha ber and all internal co ponents are anufactured fro polished stainless steel.
The shelf rack syste is designed for co plete re oval and easy access to the s ooth interior for
quick and thorough cleaning.
The outer shell of the incubator is anufactured fro zinc plated stove ena elled steel to give a
durable corrosion resistant finish.
The outer door and top cover are vacuu for ed fro ABS plastic and they provide a high level of
insulation. The keypad and display are incorporated into the top cover per itting axi u usable
cha ber volu e.

3
Section 2
Section 2Section 2
Section 2
Guidance Notes and Routine Checks
2.1 To ensure that cha ber conditions re ain as stable as possible always ini ize the length
of ti e that the inner door is open. The agnetic door catch is specifically designed to ake door
opening and closing as easy as possible. When opening the inner door, wipe off any s all drops of
condensate that ay have for ed on the inner seal. This will avoid a build up of condensation.
2.2 For cell culture work – we reco end the use of copper sulphate (or recognised biocide) in
the hu idity tray. Tests have shown that as well as inhibiting bacterial growth in the tray this
can reduce conta ination on the cha ber walls (one s all teaspoonful copper sulphate
reco ended).
2.3 For IVF and other sensitive work – we do not reco end the use of any biocide in the
hu idity tray. To reduce the possibility of conta ination, the hu idity tray water should be
changed and the hu idity tray cleaned with 70% Alcohol / 30% distilled water solution every
10 – 14 days. To re ove / replace the hu idity tray: - turn the tray through 45° and re ove
for refilling. To replace the hu idity tray, carefully turn through 45° and pass the tray through
the front of the shelf racks.
2.4 Daily
2.4.1 Check that the te perature and CO
2
levels are reading within specification.
2.4.2 Check the reserve pressure in the CO
2
cylinder (nor ally 50 bar when full). The design of
the incubator ensures very low consu ption of CO
2
and during nor al working conditions a type K
cylinder should last approxi ately 12 onths. If there is a significant drop at the cylinder 50 bar
pressure, it eans that the cylinder is al ost e pty and should be replaced, ensuring that there are
no leaks at any of the joints.
2.5 Monthly
2.5.1 If not changing the hu idity tray water / cleaning hu idity tray regularly - top up the hu idity
tray (to a axi u volu e of 0.5 litres). The use of war water (approx. 37.0°C) will ensure a rapid
return to opti u cha ber conditions. After topping-up, check that the hu idity tray is central
between the racks and pushed back against the stops.

4
Section 3
Section 3Section 3
Section 3
Installation
3.1 Unpack the incubator by lifting it fro the delivery pallet ensuring it is supported by the base.
At least two people are required for safe lifting. In the unlikely event of any transit da age this
ust be reported within 7 days.
3.2 The following ite s are supplied with your new incubator:-
3 Non-tip shelves (securely packed separately)
2 shelving racks (securely packed separately)
18 Silicone Rubber Suction Feet. Supplied packed in accessory bag
1 Hu idity tray (securely packed separately)
1 Mains power cord supplied packed in accessory bag
3 of PVC 6 bore plastic tubing supplied in accessory bag
1 CO
2
HEPA-vent filter (connected to PVC tube in accessory bag)
2 Hose clips supplied packed in accessory bag
4 Adjustable feet (To ensure adequate airflow for correct operation of the relative hu idity
control syste the incubator feet must be fitted. Feet are supplied fitted to the unit.
4 Anti-slip pads for the adjustable feet
1 Black sensor cover (in situ)
If required, further shelves can be purchased up to a axi u of 6. The hu idity tray has a fixed
position at the lowest shelf rack position. The hu idity tray should always be left in place even if the
incubator is not hu idified.
3.3 In order to use the incubator you will require:-
a) A 220/240V 50 Hz or 100/120V 50/60 Hz earthed electrical supply - ini u
capacity 3 a ps (5 a ps High Te perature Deconta ination Models).
Or
A 100/120V 50/60 Hz earthed electrical supply - ini u capacity 6 a ps (8
a ps High Te perature Deconta ination Models).
b) CO
2
gas cylinder (100% CO
2
vapour withdrawal) together with a two
stage regulator for pressure control to 0.35 BAR. (5lbf / in
2
).
c) Copper Sulphate or other biocide for the hu idity reservoir (optional).
3.4 The incubator is designed to operate at a cha ber te perature of 4.0°C above a bient and
at an absolute ini u a bient te perature of 15°C if being used at 37°C. Therefore care
should be taken to avoid placing the incubator in draughts, direct sunlight or near a radiator.

5
The operating temperature range indicated above excludes any heat generating
equipment operating within the unit. The presence on any such appliance will affect the
operational temperature characteristics.
3.5 To ensure adequate airflow for correct operation of the relative hu idity control syste the
incubator feet must be fitted to insert the adjustable feet, front pair first, tilt the incubator
backwards, screw the feet in to the required depth. Repeat the operation, tilting the incubator
forward for the rear pair.
3.6 Place the incubator in the working position. The unit is designed to be sited on a level surface
capable of bearing 32kg (approx – actual weight will depend on options fitted). It is
reco ended that a spirit level be used on the centre of the iddle shelf to position the
incubator, the feet can be individually adjusted to co pensate for uneven surfaces. Fit the
anti-slip pads to the front feet. Purpose designed stands and stacking asse blies are also
available.
3.7 Asse ble the shelf racks as follows:- Carefully re ove any packaging. Fit 7 silicone rubber
suction cup feet to the shelf racks in positions indicated on the picture below left with an ‘*’.
Repeat for the opposite shelf rack.
To fit the shelf racks to the incubator:- The racks are ‘handed’ and the suction cup arked ‘R’
goes to the rear of the cha ber as shown in the centre picture above. The suction cups will
adhere to the cha ber walls dry, but can be da pened with distilled water to increase
adhesion if required. Ensure the shelf racks are fitted squarely to the cha ber to enable the
shelves to sit on a level plane (picture above right).
3.8 Connect the CO
2
using the 6 plastic tubing to the atching CO
2
inlet on the rear of the
incubator. Use the tubing clips provided to eli inate CO
2
leaks. Turn on the gas supply with
the pressure set to 0.35 BAR (5lbf/ in2). Re ove the black protective cover fro sensor
(keep for use when disinfecting). Ensure that the white porous sensor cover re ains in place.
WARNING!
CO
2
levels.CO
2
is harmful in high concentrations. Any gases that may escape from the
unit have to be managed via adequate room ventilation or the use of an e haust system.
Check compliance with the ma imum permitted workplace concentration for CO
2
when
operating all units.

6
3.9 Connect the incubator to an earthed power supply using the power cord provided. The display
will illu inate i ediately. NB:- Do not progra e the CO
2
level yet
3.10 The cha ber set points are pre-progra ed at 37.0°C and 0% CO
2
. Leave the incubator
until the progra ed cha ber te perature has been reached.
3.11 Carefully unpack the hu idity tray. Fill with 0.5 litres of war distilled water (at approx.
37.0°C). For cell culture work – we reco end the use of copper sulphate (or recognised
biocide) in the hu idity tray (one s all teaspoonful copper sulphate reco ended).
For IVF and other sensitive work – we do not reco end the use of any biocide in the
hu idity tray. To reduce the possibility of conta ination, the hu idity tray water should be
changed and the hu idity tray cleaned with 70% Alcohol / 30% distilled water solution every
10 – 14 days. To re ove / replace the hu idity tray: - turn the tray through 45° and re ove
for refilling.. To fit the hu idity tray - Turn the hu idity tray through 45° and pull it forward as
shown in the pictures on the following page:-
NB – ensure that the Hu idity Tray is located centrally between the racks and pushed fully
back against the stop as shown above right.
3.13 Fit the shelves and leave the incubator for at least two hours (preferably overnight) to allow
conditions to stabilise.
3.14 Progra e the CO
2
level (i.e. 5%) and ensure that the CO
2
level stabilises at the
progra ed level
3.15 To carry out the Auto Zero, press the ‘∗’ and ‘↵’ keys si ultaneously. The Inner Door ust
be opened and closed according to the displayed instructions, after which the process is
co pletely auto atic.
3.16 The incubator is now ready for use.

7
Section 4
Section 4Section 4
Section 4
Setting the Humidity Conditions
If hu idification is required, the hu idity tray which is located on the lower shelf runners
should be pulled forward and filled with 0.5 litres of war distilled water for cell culture work –
we reco end the use of copper sulphate (or recognised biocide) in the hu idity tray. Tests
have shown that as well as inhibiting bacterial growth in the tray this can reduce
conta ination on the cha ber walls (one s all teaspoonful copper sulphate reco ended).
For IVF and other sensitive work – we do not reco end the use of any biocide in the
hu idity tray. To reduce the possibility of conta ination, the hu idity tray water should be
changed and the hu idity tray cleaned with 70% Alcohol / 30% distilled water solution every
10 – 14 days. To re ove / replace the hu idity tray: - turn the tray through 45° and re ove
for refilling.
DO NOT LEAVE WATER IN THE HUMIDITY TRAY IF THE INCUBATOR IS SWITCHED OFF , AS
THIS MAY RESULT IN DAMAGE TO THE CO
2
SENSOR.

8
Section 5
Section 5Section 5
Section 5
Control Panel escription
Control Panel escriptionControl Panel escription
Control Panel escription
The control panel consists of two, 3 seg ent LED displays, and 4 keys. The functions and
descriptions of these are described below.
∗ PROGRAMMING Key
+ PLUS Key
- MINUS Key
‘↵’ ACCEPT Key
The deci al points on the display for Te perature and CO
2
flash ON / OFF to signify that the Alar
Syste is not ar ed.
The picture above shows the screen in nor al operation, this is how the screen should be illu inated
when running nor ally.

9
Section 6
Section 6Section 6
Section 6
Programming Temperature, CO
Programming Temperature, COProgramming Temperature, CO
Programming Temperature, CO 2
2 2
2 & Auto Zeroing
& Auto Zeroing& Auto Zeroing
& Auto Zeroing
6.1 Programming Temperature & CO
6.1 Programming Temperature & CO6.1 Programming Temperature & CO
6.1 Programming Temperature & CO 2
22
2
The ‘∗’ Key allows Te perature and CO
2
to be progra ed within the following ranges:
Te perature Range: 10°C ( ust be 1°C above a bient) to 50°C.
CO
2
Range: 0.2% to 10%
Once the Te perature has been progra ed it ust be entered with the ‘↵’ Key, the CO
2
can then
be progra ed.
Press the ‘+ ’ and ‘-’ keys si ultaneously to enter the Engineering Mode. If this is inadvertently done,
press the ‘∗’ key to exit.
6.2 CO
6.2 CO6.2 CO
6.2 CO 2
2 2
2 Auto Zero
Auto ZeroAuto Zero
Auto Zero
The purpose of the Auto Zero syste is to reference the CO
2
Sensor to an at ospheric CO
2
level of
0.05%. Over a period of ti e the sensor zero ay drift causing an inaccuracy in the CO
2
level at the
progra ed value. It is reco ended that the CO
2
syste is Auto Zeroed onthly to ensure that
CO
2
level is correct.
To carry out the Auto Zero, press the ‘∗’ and ‘↵’ keys si ultaneously. The inner door ust be
opened and closed according to the displayed instructions, after which the process is co pletely
auto atic.
6.2.1 The door is opened for 60 seconds to co pletely degas the cha ber. A countdown is shown
on the display after which the instruction to close the door is displayed.
6.2.2 The door is then closed and a 10 inute countdown starts, to allow the cha ber conditions to
recover and the CO
2
sensor signal to stabilise, after which the sensor signal is auto atically re-
referenced. If the door is accidentally opened during the 10 inute countdown, close the door again to
reset the ti er and resu e the Auto Zero process until co plete.
6.2.3 The incubator will now re-open the CO
2
valve to allow the CO
2
level to recover to the
Progra e value.
6.2.4 Pressing the ‘ ∗ ’ key will auto atically ter inate the Auto Zero. Pressing any other key or
opening the door during the Auto Zero will not ter inate it.