SCIEX ExionLC Manual

ExionLC™PDA Detector
Operator Guide
October 2015RUO-IDV-05-1842-B

This document is provided to customers who have purchased SCIEX equipment to use in the operation of such SCIEX
equipment. This document is copyright protected and any reproduction of this document or any part of this document is
strictly prohibited, except as SCIEX may authorize in writing.
Software that may be described in this document is furnished under a license agreement. It is against the law to copy, modify,
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agreement may prohibit the software from being disassembled, reverse engineered, or decompiled for any purpose. Warranties
are as stated therein.
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For research use only. Not for use in diagnostic procedures.
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©2015 AB SCIEX
AB Sciex Pte. Ltd.
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Chapter 1 Introduction...............................................................................................................................5
Electrical Precautions.........................................................................................................................................................5
AC Mains Supply..........................................................................................................................................................5
Protective Earth Conductor..........................................................................................................................................6
Environmental Precautions................................................................................................................................................6
Electromagnetic Environment......................................................................................................................................6
Decommissioning and Disposal....................................................................................................................................6
Ventilation Precautions......................................................................................................................................................7
Chemical Precautions.........................................................................................................................................................7
Static Electricity Precautions .............................................................................................................................................7
Equipment Use and Modification.......................................................................................................................................8
Chapter 2 Hazard Symbols.........................................................................................................................9
Occupational Health and Safety Symbols...........................................................................................................................9
Documentation Symbols and Conventions.......................................................................................................................10
Chapter 3 Overview..................................................................................................................................12
Chapter 4 Operating Instructions............................................................................................................17
Precautions......................................................................................................................................................................17
Preparation Before Operation....................................................................................................................................17
Precautions for Wavelength Calibration and Wavelength Accuracy Check................................................................18
Precautions During Operation....................................................................................................................................18
Turn On the Module.........................................................................................................................................................18
Chapter 5 Service and Maintenance Information...................................................................................20
Maintenance, Inspections, and Adjustment.....................................................................................................................20
Prior to Inspection and Maintenance.........................................................................................................................20
Periodic Inspection and Maintenance List..................................................................................................................21
Clean or Replace the Flow Cell........................................................................................................................................22
Remove the Flow Cell................................................................................................................................................22
Clean the Flow Cell....................................................................................................................................................23
Install the Flow Cell...................................................................................................................................................27
Replace the D2 Lamp.......................................................................................................................................................28
Replace the Dust Filter.....................................................................................................................................................31
Clean the Module Surfaces..............................................................................................................................................33
Chapter 6 Troubleshooting......................................................................................................................34
Corrective Action for Bubbles..........................................................................................................................................39
Insulation Tube................................................................................................................................................................39
Corrective Action against Leaks from Cell Window.........................................................................................................40
Appendix A Error Messages.....................................................................................................................41
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Appendix B Status Panel..........................................................................................................................44
Appendix C Consumables and Spares.....................................................................................................46
Revision History........................................................................................................................................47
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Contents

This guide describes the basic operation and troubleshooting for the ExionLCTM PDA Detector . Read this guide
thoroughly before using the product and operate the product in accordance with the instructions in this guide.
This guide provides safety instructions and precautions to make sure that the user operates the system safely.
Follow all Warning and Caution instructions provided in the guide. For more information, refer to the Hardware
User Guide for the ExionLCTM system.
Keep this guide for future reference. Make sure that it is accessible to the operator of the system.
Electrical Precautions
WARNING! Electrical Shock Hazard. Do not remove the covers. Removing the covers
might cause injury or malfunctioning of the system. The covers need not be removed
for routine maintenance, inspection, or adjustment. Contact the SCIEX FSE for repairs
that require the covers to be removed.
For information on system electrical specifications, refer to the Site Planning Guide.
AC Mains Supply
Connect the system to a compatible AC mains supply as instructed in this guide.
WARNING! Electrical Shock Hazard. Use only qualified personnel for the installation
of all electrical supplies and fixtures, and make sure that all installations adhere to
local regulations and safety standards.
WARNING! Electrical Shock Hazard. Make sure that the system can be disconnected
from the AC mains supply outlet in an emergency. Do not block the AC mains supply
outlet.
CAUTION: Potential System Damage. Do not unpack or connect any system components.
The FSE will unpack, connect, and configure the system for the proper operating voltage.
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Introduction

Protective Earth Conductor
The mains supply must include a correctly installed protective earth conductor. The protective earth conductor
must be installed or checked by a qualified electrician before the system is connected.
WARNING! Electrical Shock Hazard. Do not intentionally interrupt the protective
earth conductor. Any interruption of the protective earth conductor will create an
electrical shock hazard.
Environmental Precautions
Use qualified personnel for the installation of electrical mains, heating, ventilation, and plumbing supplies and
fixtures. Make sure that all of the installations comply with local bylaws and biohazard regulations. For more
information about the required environmental conditions for the system, refer to the Site Planning Guide.
WARNING! Biohazard. For biohazardous material use, always comply with local
regulations for hazard assessment, control, and handling. This system or any part is
not intended to act as a biological containment.
Electromagnetic Environment
CAUTION: Potential Wrong Result. Do not use this device in close proximity to sources of
strong electromagnetic (EMC) radiation (for example, unshielded intentional RF sources),
as EMC radiation might interfere with the proper operation and cause a wrong result.
Make sure that a compatible electromagnetic environment for the equipment can be maintained so that the device
will perform as intended.
Decommissioning and Disposal
Before decommissioning, decontaminate the system following local regulations.
When removing the system from service, separate and recycle different materials according to national and local
environmental regulations.
Do not dispose of system components or subassemblies, including computer parts, as unsorted municipal waste.
Follow local municapal waste ordinances for proper disposal provisions to reduce the environmental impact of
WEEE (waste, electrical and electronic equipment). To safely dispose of this equipment, contact a local Customer
Service office for complimentary equipment pickup and recycling.
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Introduction

Note: SCIEX will not accept any system returns without a completed Decontamination Form. Contact an FSE
to obtain a copy of the form.
Ventilation Precautions
The venting of fumes and disposal of waste must comply with all of the federal, state, provincial, and local health
and safety regulations. It is the customer's responsibility to make sure that the air quality is maintained in compliance
with local health and safety regulations.
Chemical Precautions
•Determine which chemicals have been used in the system prior to service and regular maintenance. Refer to
Safety Data Sheets for the health and safety precautions that must be followed with chemicals.
•Work in a well-ventilated area.
•Always wear assigned personal protective equipment, including powder-free neoprene or nitrile gloves, safety
glasses, and a laboratory coat.
•Follow required electrical safe work practices.
•Avoid ignition sources when working with flammable materials, such as isopropanol, methanol, and other
flammable solvents.
•Take care in the use and disposal of any chemicals. Potential risk of personal injury if proper procedures for
handling and disposing of chemicals are not followed.
•Avoid skin contact with chemicals during cleaning and wash hands after use.
•Comply with all of the local regulations for the storage, handling, and disposal of biohazardous, toxic, or
radioactive materials.
•(Recommended) Use secondary containment trays beneaththe solvent bottles and the waste collection container
to capture potential chemical spills.
Static Electricity Precautions
Liquid chromatography (LC) uses flammable organic solvents as the mobile phase. LC systems are also often used
where large amount of flammable substances are present. Thus, there is a risk of accidents involving fire or
explosion.
The major cause of these accidents is static electricity. Devising preventative measures for static electricity can be
difficult, because the symptoms before an accident vary and can be hard to detect, because such accidents occur
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Introduction

as a result of several simultaneous incidents. For recommended methods for preventing static electricity accidents,
refer to the Hardware User Guide for the ExionLCTM system.
Equipment Use and Modification
WARNING! Electrical Shock Hazard. Do not remove the covers. Removing the covers
might cause injury or malfunctioning of the system. The covers need not be removed
for routine maintenance, inspection, or adjustment. Contact the SCIEX FSE for repairs
that require the covers to be removed.
WARNING! Personal Injury Hazard. Use SCIEX-recommended parts only. Use of parts
not recommended by SCIEX or use of parts for any use other than their intended
purpose can place the user at risk of harm or negatively impact system performance.
Use the system indoors in a laboratory that complies with the environmental conditions recommended in the Site
Planning Guide.
If the system is used in an environment or in a manner not prescribed by the manufacturer, then the protection
provided by the equipment might be impaired.
Unauthorized modification or operation of the system might cause personal injury and equipment damage, and
might void the warranty. Erroneous data might be generated if the system is operated either above or below the
recommended environmental conditions or operated with unauthorized modifications. Contact an FSE for information
on servicing the system.
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Introduction

This section lists the hazard symbols and conventions used in the laboratory environment, on the system, and in
the documentation.
Occupational Health and Safety Symbols
This section describes some occupational health and safety symbols found in the documentation and laboratory
environment.
Table 2-1 General Hazard Symbols
DescriptionSafety Symbol
Personal Injury Hazard
Table 2-2 Chemical Hazard Symbols
DefinitionSafety Symbol
Biohazard
Explosion Hazard
Toxic Chemical Hazard
Fire Hazard
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Hazard Symbols

Table 2-3 Electrical Hazard Warning Symbols
DefinitionSafety Symbol
Electrical Shock Hazard
Table 2-4 Mechanical Hazard Symbols
DefinitionSafety Symbol
Hot Surface Hazard
Table 2-5 Radiation Hazard Symbols
DefinitionSafety Symbol
Laser Radiation Hazard
Documentation Symbols and Conventions
The following symbols and conventions are used throughout the guide.
DANGER! Danger signifies an action which leads to severe injury or death.
WARNING! Warning signifies an action that could cause personal injury if precautions
are not followed.
CAUTION: Caution signifies an operation that could cause damage to the system or
corruption or loss of data if precautions are not followed.
Note: Note emphasizes significant information in a procedure or description.
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Hazard Symbols