SCIEX ExionLC Manual

ExionLC™PDA Detector
Operator Guide
October 2015RUO-IDV-05-1842-B

This document is provided to customers who have purchased SCIEX equipment to use in the operation of such SCIEX
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Chapter 1 Introduction...............................................................................................................................5
Electrical Precautions.........................................................................................................................................................5
AC Mains Supply..........................................................................................................................................................5
Protective Earth Conductor..........................................................................................................................................6
Environmental Precautions................................................................................................................................................6
Electromagnetic Environment......................................................................................................................................6
Decommissioning and Disposal....................................................................................................................................6
Ventilation Precautions......................................................................................................................................................7
Chemical Precautions.........................................................................................................................................................7
Static Electricity Precautions .............................................................................................................................................7
Equipment Use and Modification.......................................................................................................................................8
Chapter 2 Hazard Symbols.........................................................................................................................9
Occupational Health and Safety Symbols...........................................................................................................................9
Documentation Symbols and Conventions.......................................................................................................................10
Chapter 3 Overview..................................................................................................................................12
Chapter 4 Operating Instructions............................................................................................................17
Precautions......................................................................................................................................................................17
Preparation Before Operation....................................................................................................................................17
Precautions for Wavelength Calibration and Wavelength Accuracy Check................................................................18
Precautions During Operation....................................................................................................................................18
Turn On the Module.........................................................................................................................................................18
Chapter 5 Service and Maintenance Information...................................................................................20
Maintenance, Inspections, and Adjustment.....................................................................................................................20
Prior to Inspection and Maintenance.........................................................................................................................20
Periodic Inspection and Maintenance List..................................................................................................................21
Clean or Replace the Flow Cell........................................................................................................................................22
Remove the Flow Cell................................................................................................................................................22
Clean the Flow Cell....................................................................................................................................................23
Install the Flow Cell...................................................................................................................................................27
Replace the D2 Lamp.......................................................................................................................................................28
Replace the Dust Filter.....................................................................................................................................................31
Clean the Module Surfaces..............................................................................................................................................33
Chapter 6 Troubleshooting......................................................................................................................34
Corrective Action for Bubbles..........................................................................................................................................39
Insulation Tube................................................................................................................................................................39
Corrective Action against Leaks from Cell Window.........................................................................................................40
Appendix A Error Messages.....................................................................................................................41
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Appendix B Status Panel..........................................................................................................................44
Appendix C Consumables and Spares.....................................................................................................46
Revision History........................................................................................................................................47
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Contents

This guide describes the basic operation and troubleshooting for the ExionLCTM PDA Detector . Read this guide
thoroughly before using the product and operate the product in accordance with the instructions in this guide.
This guide provides safety instructions and precautions to make sure that the user operates the system safely.
Follow all Warning and Caution instructions provided in the guide. For more information, refer to the Hardware
User Guide for the ExionLCTM system.
Keep this guide for future reference. Make sure that it is accessible to the operator of the system.
Electrical Precautions
WARNING! Electrical Shock Hazard. Do not remove the covers. Removing the covers
might cause injury or malfunctioning of the system. The covers need not be removed
for routine maintenance, inspection, or adjustment. Contact the SCIEX FSE for repairs
that require the covers to be removed.
For information on system electrical specifications, refer to the Site Planning Guide.
AC Mains Supply
Connect the system to a compatible AC mains supply as instructed in this guide.
WARNING! Electrical Shock Hazard. Use only qualified personnel for the installation
of all electrical supplies and fixtures, and make sure that all installations adhere to
local regulations and safety standards.
WARNING! Electrical Shock Hazard. Make sure that the system can be disconnected
from the AC mains supply outlet in an emergency. Do not block the AC mains supply
outlet.
CAUTION: Potential System Damage. Do not unpack or connect any system components.
The FSE will unpack, connect, and configure the system for the proper operating voltage.
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Introduction

Protective Earth Conductor
The mains supply must include a correctly installed protective earth conductor. The protective earth conductor
must be installed or checked by a qualified electrician before the system is connected.
WARNING! Electrical Shock Hazard. Do not intentionally interrupt the protective
earth conductor. Any interruption of the protective earth conductor will create an
electrical shock hazard.
Environmental Precautions
Use qualified personnel for the installation of electrical mains, heating, ventilation, and plumbing supplies and
fixtures. Make sure that all of the installations comply with local bylaws and biohazard regulations. For more
information about the required environmental conditions for the system, refer to the Site Planning Guide.
WARNING! Biohazard. For biohazardous material use, always comply with local
regulations for hazard assessment, control, and handling. This system or any part is
not intended to act as a biological containment.
Electromagnetic Environment
CAUTION: Potential Wrong Result. Do not use this device in close proximity to sources of
strong electromagnetic (EMC) radiation (for example, unshielded intentional RF sources),
as EMC radiation might interfere with the proper operation and cause a wrong result.
Make sure that a compatible electromagnetic environment for the equipment can be maintained so that the device
will perform as intended.
Decommissioning and Disposal
Before decommissioning, decontaminate the system following local regulations.
When removing the system from service, separate and recycle different materials according to national and local
environmental regulations.
Do not dispose of system components or subassemblies, including computer parts, as unsorted municipal waste.
Follow local municapal waste ordinances for proper disposal provisions to reduce the environmental impact of
WEEE (waste, electrical and electronic equipment). To safely dispose of this equipment, contact a local Customer
Service office for complimentary equipment pickup and recycling.
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Introduction

Note: SCIEX will not accept any system returns without a completed Decontamination Form. Contact an FSE
to obtain a copy of the form.
Ventilation Precautions
The venting of fumes and disposal of waste must comply with all of the federal, state, provincial, and local health
and safety regulations. It is the customer's responsibility to make sure that the air quality is maintained in compliance
with local health and safety regulations.
Chemical Precautions
•Determine which chemicals have been used in the system prior to service and regular maintenance. Refer to
Safety Data Sheets for the health and safety precautions that must be followed with chemicals.
•Work in a well-ventilated area.
•Always wear assigned personal protective equipment, including powder-free neoprene or nitrile gloves, safety
glasses, and a laboratory coat.
•Follow required electrical safe work practices.
•Avoid ignition sources when working with flammable materials, such as isopropanol, methanol, and other
flammable solvents.
•Take care in the use and disposal of any chemicals. Potential risk of personal injury if proper procedures for
handling and disposing of chemicals are not followed.
•Avoid skin contact with chemicals during cleaning and wash hands after use.
•Comply with all of the local regulations for the storage, handling, and disposal of biohazardous, toxic, or
radioactive materials.
•(Recommended) Use secondary containment trays beneaththe solvent bottles and the waste collection container
to capture potential chemical spills.
Static Electricity Precautions
Liquid chromatography (LC) uses flammable organic solvents as the mobile phase. LC systems are also often used
where large amount of flammable substances are present. Thus, there is a risk of accidents involving fire or
explosion.
The major cause of these accidents is static electricity. Devising preventative measures for static electricity can be
difficult, because the symptoms before an accident vary and can be hard to detect, because such accidents occur
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Introduction

as a result of several simultaneous incidents. For recommended methods for preventing static electricity accidents,
refer to the Hardware User Guide for the ExionLCTM system.
Equipment Use and Modification
WARNING! Electrical Shock Hazard. Do not remove the covers. Removing the covers
might cause injury or malfunctioning of the system. The covers need not be removed
for routine maintenance, inspection, or adjustment. Contact the SCIEX FSE for repairs
that require the covers to be removed.
WARNING! Personal Injury Hazard. Use SCIEX-recommended parts only. Use of parts
not recommended by SCIEX or use of parts for any use other than their intended
purpose can place the user at risk of harm or negatively impact system performance.
Use the system indoors in a laboratory that complies with the environmental conditions recommended in the Site
Planning Guide.
If the system is used in an environment or in a manner not prescribed by the manufacturer, then the protection
provided by the equipment might be impaired.
Unauthorized modification or operation of the system might cause personal injury and equipment damage, and
might void the warranty. Erroneous data might be generated if the system is operated either above or below the
recommended environmental conditions or operated with unauthorized modifications. Contact an FSE for information
on servicing the system.
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Introduction

This section lists the hazard symbols and conventions used in the laboratory environment, on the system, and in
the documentation.
Occupational Health and Safety Symbols
This section describes some occupational health and safety symbols found in the documentation and laboratory
environment.
Table 2-1 General Hazard Symbols
DescriptionSafety Symbol
Personal Injury Hazard
Table 2-2 Chemical Hazard Symbols
DefinitionSafety Symbol
Biohazard
Explosion Hazard
Toxic Chemical Hazard
Fire Hazard
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Hazard Symbols

Table 2-3 Electrical Hazard Warning Symbols
DefinitionSafety Symbol
Electrical Shock Hazard
Table 2-4 Mechanical Hazard Symbols
DefinitionSafety Symbol
Hot Surface Hazard
Table 2-5 Radiation Hazard Symbols
DefinitionSafety Symbol
Laser Radiation Hazard
Documentation Symbols and Conventions
The following symbols and conventions are used throughout the guide.
DANGER! Danger signifies an action which leads to severe injury or death.
WARNING! Warning signifies an action that could cause personal injury if precautions
are not followed.
CAUTION: Caution signifies an operation that could cause damage to the system or
corruption or loss of data if precautions are not followed.
Note: Note emphasizes significant information in a procedure or description.
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Hazard Symbols

Tip! Tip provides useful information that helps apply the techniques and procedures in the text for a specific
need and provides shortcuts, but is not essential to the completion of a procedure.
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Hazard Symbols

The ExionLCTM PDA Detector is a photodiode array (PDA), UV-Vis detector designed for use with an ExionLCTM
ultra high performance liquid chromatograph system (HPLC), and was developed to satisfy demands for greater
accuracy and sensitivity in analysis.
A photodiode array detector continuously monitors spectra over the entire wavelength range. Use of a deuterium
(D2) lamp as the light source allows high sensitivity measurement of chromatograms and absorbance spectra over
a wavelength range of 190 nm to 700 nm.
•Continuous monitoring of the absorbance spectra of eluted components can improve analysis reliability.
•Identification of components from absorbance spectra
The combined use of retention time with absorbance spectra provides greater accuracy in identification.
•Impurities check
Eluted peaks can be inspected to determine whether they are generated from a single component or mixed
with impurities.
•An improved optical system provides high spectral resolution and excellent S/N performance.
A variable slit is incorporated to enable even higher sensitivity according to the application.
•Polychomator temperature control reduces baseline drift caused by fluctuations in room temperature.
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3
Overview

Figure 3-1 Front
DescriptionItem
Display. Shows the statuses of lamps and the module.1
Dust filter. Prevents dust from being sucked into the external air inlet, which draws in air to cool
the inside of the module. Refer to Replace the Dust Filter.
2
Front cover. Open this cover to install or perform maintenance on the flow cell.3
Power switch. Turns the power on and off.4
Left-side cover. Open this cover to replace the lamp.5
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Overview

Figure 3-2 Behind the Front Cover and Left-side Cover
FunctionNameItem
Remove the lamp cover to replace the D2 lamp inside. Refer to Replace the
D2 Lamp.
When the lamp cover is removed, the lamp is automatically turned off to
ensure safety.
Lamp cover1
Connects to the flow cell.Flow cell connector2
Secures the tubing.Tubing holder3
The retaining plate and screw secure the coupling in place.Coupling holder4
Installation is performed using the coupling.Flow cell5
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Overview

Figure 3-3 Base Panel
FunctionNameItem
Connects to the provided drain tubing. If liquid leaks inside the module, it is
drained out through this opening.
Leakage drain outlet1
Protects the internal measurement components from vibration during
transport. It is removed after installation.
Shipping screw2
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Overview

Figure 3-4 Back
FunctionNameItem
Used for the interface of control signals and analysis data through the network.ETHERNET connector1
Reserved for use by service personnel.RS-232C connector2
Used for the input and output of analysis start and stop signals, and event
signals when an error occurs. These are used to connect to external equipment.
Refer to the Peripheral Devices Setup Guide for the Analyst®software.
External
input/output
terminals
3
Not applicable.INTEGRATOR
connectors (four
channels)
4
Contains the fuses.Fuse holders5
Connector for the power cable.Power cord
connector
6
Fan for internal cooling (keep a minimum of 100 mm between the rear of the
module and the wall.)
Cooling fan7
Used to initialize the module.INITIALIZE button8
Press this button to clear errors.CLEAR button9
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Overview

Precautions
Preparation Before Operation
•After starting up the module or turning on the lamp, it takes some time for the baseline to stabilize.
When high sensitivity is required, turn on the lamp in advance while taking into account the time required for
baseline stabilization. As a guide, stabilization takes about 1.5 hours following startup.
•To prevent liquid leakage from the flow cell, tighten the cell window fixing screw on the flow cell every six
months.
Refer to Corrective Action against Leaks from Cell Window.
Fittings
Always use the fittings provided with the module. Otherwise, interior parts of the cell might be damaged due to
high pressure generated within the cell when the cell plumbing is clogged.
Note: Any damage to the cell resulting from the use of fittings other than those provided voids the guarantee.
Refer to:
•Plumbing Between Column and Detector in the Hardware User Guide.
•Remove the Flow Cell
•Install the Flow Cell
Performance Checks
Perform these inspections daily to maintain module performance:
•Flow cell bubble removal and liquid leakage check
Refer to Corrective Action against Leaks from Cell Window.
•Lamp light intensity check
•Wavelength accuracy check
Refer to Service and Maintenance Information.
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Operating Instructions

Precautions for Wavelength Calibration and Wavelength
Accuracy Check
When conducting wavelength calibration or a wavelength accuracy check, perform one of the following flow cell
preparation procedures.
•Flush the flow cell with a mobile phase that does not exhibit absorption in the wavelength region above 230
nm (such as water, acetonitrile, methanol).
•Fill the flow cell with one of these mobile phases.
Note: The wavelength calibration and wavelength accuracy check include measurement of the lamp light
intensity as standard at the D2 lamp emission line wavelengths (656.1 nm, 486.0 nm) and holmium oxide filter
absorption wavelengths (360.8 nm, 287.6 nm, 241.5 nm). If the flow cell contains air bubbles or residue from a
light absorbing sample, wavelength calibration and wavelength accuracy check might not operate properly.
Precautions During Operation
•Be sure to close the cover during measurement. Opening the left or front covers during high sensitivity
measurement might cause an increase in baseline fluctuation and noise.
Precautions for Air Conditioner
•When performing high sensitivity measurement, turn on the power to the PDA Detector after the room
temperature is stabilized.
•Furthermore, when using a room air conditioner, keep it running during analysis to maintain a constant room
temperature.
Note: The PDA Detector controls the temperature of the optical system to reduce absorbance baseline drift due
to room temperature fluctuation. The detector measures ambient temperature for 15 minutes after the power
is turned on and defines the temperature control level according to the measured temperature. If the room
temperature is unstable after the power is turned on, the control temperature might be inaccurate and effects
of room temperature change such as baseline drift in sync with temperature fluctuation, might appear during
analysis. In addition, if the air conditioner is turned off, or if for some other reason the room temperature changes
too much, the temperature control on the optical system might become inadequate.
Turn On the Module
•Press the power switch.
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Operating Instructions

Figure 4-1 Power Button
The following events occur:
a. The status indicator light is green and the D2 and CONNECT LEDs begin blinking alternately. While this
is occurring, the module performs the motor home position detection and slit initialization operations.
b. The D2 LED blinks more quickly and the light source lamp starts to turn on. The CONNECT LED illuminates
when the module establishes communications with the system controller and acquisition computer.
c. After the light source lamp is illuminated, the D2 LED stops blinking and illuminates continuously.
Figure 4-2 Display
DescriptionItem
Green1
Orange2
Green blink3
Fast Green blinking4
Green or off5
Note: Whether the D2 lamp is turned on or not is determined by the setting at the time of the last module
shutdown. If an error occurs, the status indicator turns red.
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Operating Instructions

Regularly clean and maintain the system for optimal performance. Refer to Periodic Inspection and Maintenance
List on page 21.
WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Determine
whether mass spectrometer decontamination is required prior to cleaning or
maintenance. The customer must decontaminate the system prior to cleaning
or maintenance if radioactive materials, biological agents, or toxic chemicals
have been used with a mass spectrometer.
Maintenance, Inspections, and Adjustment
WARNING! Personal Injury Hazard. Contact the SCIEX representative if product
installation, adjustment, or relocation is required.
WARNING! Electrical Shock Hazard. Always turn off the power and then unplug the
instrument prior to performing inspection and maintenance. Otherwise, fire, electric
shock, or a malfunction might occur.
To maintain the performance of the module system and to obtain accurate measurement data, perform daily
inspection and periodic calibration.
•For daily maintenance and inspection, refer to Precautions.
•For planned maintenance, contact a SCIEX representative.
•For replacement parts, refer to Consumables and Spares.
•Replacement cycles described for periodic replacement parts are estimates. Replacement might be required
earlier than the described replacement cycles depending on usage environment and frequency.
Prior to Inspection and Maintenance
•Purge the mobile phase in the flow lines with water.
•Wipe away any dirt from the front panel and the main cover.
•Wipe away any dirt from the screen with tissue paper or a soft cloth moistened with water.
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5
Service and Maintenance
Information