ICELL8® Imaging System User Manual
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Page 2 of 18
Table of Contents
I. Preface............................................................................................................................................................................. 3
II. Introduction..................................................................................................................................................................... 4
III. Startup......................................................................................................................................................................... 7
A. Starting the Hardware ................................................................................................................................................. 7
B. Starting the Software................................................................................................................................................... 8
IV. Operation..................................................................................................................................................................... 9
A. Setting Up the Chip..................................................................................................................................................... 9
B. Focusing on Cells...................................................................................................................................................... 12
C. Acquiring Images...................................................................................................................................................... 17
D. System Shutdown and Next Steps ............................................................................................................................ 17
Appendix A: Maintenance and Troubleshooting .................................................................................................................. 18
Table of Figures
Figure 1. Software interface for cell imaging. ........................................................................................................................ 4
Figure 2. Workflow diagram depicting single-cell isolation and imaging steps..................................................................... 5
Figure 3. ICELL8 Imaging System Components.................................................................................................................... 6
Figure 4. Starting the Mercury Burner.................................................................................................................................... 7
Figure 5. Starting the Camera. ................................................................................................................................................ 7
Figure 6. Turning on the Filter Controller and Stage Controller. ........................................................................................... 7
Figure 7. Starting the Micro-Manager software: Copyright window and Main window........................................................ 8
Figure 8. Opening the Microscope shutter.............................................................................................................................. 8
Figure 9. Main window and Live window.............................................................................................................................. 9
Figure 10. Main window and Multi-Dimensional Acquisition window. ................................................................................ 9
Figure 11. Live window with image of 6 x 6 well region..................................................................................................... 10
Figure 12. Multi-Dimensional Acquisition window. ............................................................................................................ 11
Figure 13. Seating a chip on the microscope holding platform. ........................................................................................... 11
Figure 14. Positioning the ocular tube over the chip. ........................................................................................................... 12
Figure 15. Moving the Motorized Stage to Position 0.......................................................................................................... 12
Figure 16. Setting the illumination channel to DAPI............................................................................................................ 12
Figure 17. Adjusting camera depth. ...................................................................................................................................... 13
Figure 18. Adjusting contrast................................................................................................................................................ 13
Figure 19. Adjusting microscope focus. ............................................................................................................................... 14
Figure 20. Finding a focal point for single cells. .................................................................................................................. 14
Figure 21. Moving the Motorized Stage to Position 143. ..................................................................................................... 15
Figure 22. Setting the illumination channel to Texas Red. ................................................................................................... 15
Figure 23. Brightly lit nanowells containing fiducials.......................................................................................................... 15
Figure 24. Checking cell-stain channels. .............................................................................................................................. 16
Figure 25. Entering chip number and directory location for images. ...................................................................................16
Figure 26. Acquisition of field of view images with DAPI and Texas Red filters. .............................................................. 17
Figure 27. Chip Holder. ........................................................................................................................................................ 17
