TAN Bead Maelstrom 9600 LH User manual

"TAN Bead” Nucleic Acid Extractor
(Non-Sterile)
Maelstrom 9600 LH
User Manual

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Table of Contents
1.
Introduction .................................................................. 4
2.
Overview ...................................................................... 8
3.
Installation .................................................................. 11
4.
Get started.................................................................. 11
5.
Edit program ............................................................... 14
6.
Communication .......................................................... 21
7.
Product Performance……………………………………24
8.
Cleaning and maintenance ........................................ 26
9.
Disposal...................................................................... 26
10.
Patent ......................................................................... 27
11.
Appendix ................................................................... 27
Revised: V1.0_202201

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About this manual
The label on the instrument, the User Manual, and other packaging material
may contain the following symbols:
The CE mark
In Vitro Diagnostic Medical Device
Authorized European Representative
Caution, consult accompanying documents
Hot surface, risk of burns
Watch your fingers and your hands
The symbol indicates that the product should not be discarded as unsorted
waste but must sent to separate collection facilities for recovery and
recycling
Catalog number
Serial number
Manufacturer
The date of manufacture
Non-Sterile
Temp Limit
(Operating)
Biologic Risks
Keep Dry
Keep Away From Sunlight
Consult Instructions for Use
Humidity Limit (Operating)

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1. Introduction
Maelstrom 9600 LH is an automated nucleic acid platform designed for high-
throughput applications. Specialized spin tips enable super mixing efficiency
of magnetic beads and larger processing volume. With intuitive interface and
flexible program, Maelstrom 9600 LH can boost laboratory productivity by
transforming routine operations into a walk-away solution.
Operation Principle
Maelstrom 9600 LH uses magnetic rods to collect and transfer magnetic
beads and spin tips to mix suspension. Rotating the spin tips will mix
suspension. Extended magnetic rods can collect magnetic beads efficiently.
Purified nucleic acids can be obtained after lysis, nucleic acids absorption,
wash and elution.
■ Flow chart for viral DNA/DNA extraction procedure
Intended Use:
 Maelstrom 9600 LH can be used in combination with TANBead Nucleic
Acid Extraction Kit to extract DNA/RNA from various human specimen
such as whole blood plasma or serum.
 Maelstrom 9600 LH can handle magnetic beads for purification of
nucleic acid.
Environmental Requirements
To avoid shortening of the lifespan of the instrument, use Maelstrom 9600
LH in a location that meets the following criteria:
 Choose a location that has good air circulation.

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 Place Maelstrom 9600 LH on the table that can bear the weight of
Maelstrom 9600 LH (30 kg).
 Do not use Maelstrom 9600 LH in a location where there is large
temperature variability or high humidity.
 Temperature: 4-40℃
 Maximum operating altitude: 2000 m
 Storage and transportation condition:
 Temperature: 4-60℃
 Humidity: 0-85%

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Safety instructions and guidelines
 This device can be used with potentially biohazardous materials. Use
appropriate personal protective equipment (gloves, safety goggles, lab
coat, etc.) for handling and disposing of biohazardous materials.
 Under normal condition, sound pressure level from Maelstrom 9600 LH
does not exceed 80 dB and cause a hazard. Please contact technical
support in case of higher sound pressure level.
 This device can be hazardous due to the use of chemical and
biohazardous substances.
 Users should adhere to their institutional guidelines for the handling and
disposal of all infectious substances used with this device.
 It is important to clean the device after every use. If samples or reagents
have been spilled, it is important to clean the instrument immediately to
avoid damage or contamination of samples.
 This device is to use with the compatible spin tips. Using incompatible
spin tips may cause poor extraction performance.
 Read this user manual in its entirety prior to operating the device.
Failure to read, understand, and follow the instructions in the manual
may result in damage to the device, injury to laboratory and operating
personnel or poor performance.

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Equipment list
No. Item
1 Power cord
2 A plate of spin tips
3 Magnetic separator
4 User Manual

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2. Overview
 Specifications
Model: Maelstrom 9600 LH
Weight: 30 kg
Dimensions: (L) 54.2 x (W) 28.5 x (H) 26.8 cm
Controller dimensions: 32 x 30 x 14 cm
Power supply: 100-240 V
Capacity: 96 samples per run
Process. volume:
50 μl – 1500 μl
Spin speed: 500 - 3000 rpm
Heater: 1 heated plates
Magnetic rod: 3500 gauss
Display: 240 64 Dots TFT LCD
 Back view of controller:

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 Side view of extraction platform:
 Shield plate:

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 Regulators of the sensors for detecting falling spin tip:
 Control panel:
 Buttons on control panel:

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3. Installation
1. Controller and extraction platform are to be connected by 25-pin power
cable and 25-pin signal line connectors.
2. Connect electrical inlet of controller to an electrical outlet.
4. Get started
Turn on the main power switch (located on the back of controller) to the (on)
position. Once Maelstrom 9600LH is switched on, the instrument will perform
a self-test and calibration. After booting, you will see a menu showing the list
of programs in the controller:
The following example operation procedure will help you understand:
The Display of the Controller The Following Operation Procedure
Select the
Program

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Set the
Temperature
UV LAMP
Confirm the
Selected
Program
Mount the
Spin Tips

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Eject the
Spin Tips
STATUS:
RUNNING
STATUS:
PAUSE
STATUS:
STOP ?
STATUS:
END

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5. Edit program
Programs (protocol) can be edited in PC and be transferred to the controller
via AutoMag (Version 5.2) program.
5.1 Connection
(1) Switch on Maelstrom 9600 LH.
(2) Connect (A) of transmission cable to the Maelstrom 9600 LH controller.
(Fig 2)
(3) Connect (B) to the USB port of PC. (Fig 3)
Fig 1: RS232 transmission cable
Fig 2: RS232 port of Maelstrom 9600 LH Fig 3: USB port of PC

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5.2 To start AutoMag software
(A)
Enter letters as User name. (maximum characters: 10)
(B)
Enter “0” as a default Password. (maximum characters: 8)
(C)
COM port: In drop-down menu, select the used USB port.
NOTE: The used USB port can be checked in window system control
/device manage/port.
(D)
Controller version: In drop-down menu, select “2D_M16” and click
Enter button to start AutoMag software.

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5.3 Edit a program by AutoMag software
Parameter Name Function
Program No File name (00~99)
Program Name Program name (maximum characters: 15)
Plate Plate position (1-8, 0 is program end.)
Tip Mount/Eject spin tips (ON=Mount, OFF=Eject)
Pre-Heat Warm up temperature (0-150℃)
Heating Execute program temperature (0-150℃)
Mixing(M) Spin time (0-999 minutes)
Speed(RPM) Spin speed (500-3000 RPM)
Shuttle Spin at the lower and the upper alternately.
Collect(s) Collect magnetic beads (0-999 second)
Volume
Buffer volume (50-1500μl)
Vapor(M) Stand for alcohol vaporization (0-999 minutes)
Pause Pause and wait to restart by user for adding
buffer/ enzyme or change reaction plate.

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 NOTE:

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Sample programs:
(1) SHORTTEST
STEP1: The extraction head will mount spin tips on the left half part of a spin
tip rack. After mounting the spin tips, the user can replace the spin tip rack
with a reagent deep well plate. After placement of reagent deep well plate,
press “Pause” to continue.
STEP2: The 9600 LH will perform mixing for 0.1 minutes and collecting for 10
seconds in plate 1. After that, it will move out the plate holder and beep
(Pause: ON setting). The user can place the next reagent deep well plate and
press “Pause” button to continue.
STEP3: The 9600 LH will perform mixing for 0.1 minutes and collecting for 10
seconds in plate 2. After that, it will move out the plate holder and beep
(Pause: ON setting). The user can place the next reagent deep well plate and
press “Pause” button to continue.
STEP4: The 9600 LH will perform mixing for 0.1 minutes and collecting for 5
seconds in plate 3. After that, it will move out the plate holder and beep
(Pause: ON setting). The user can place the next reagent deep well plate and
press “Pause” button to continue.
STEP5: The 9600 LH will perform mixing for 0.1 minutes and collecting for 10
seconds in plate 4. After that, it will move out the plate holder and beep
(Pause: ON setting). The user can place the next reagent deep well plate and
press “Pause” button to continue.
STEP6: The 9600 LH will perform mixing for 0.1 minutes and ejection of spin
tips in the left half part of reagent deep well plate.
(2) EJECT-TIP
STEP1: The extraction head will mount spin tips from the left half part of spin
tip rack. After mounting of spin tips, it will move out plate holder. The user can
take away the spin tip rack and place an empty deep well plate. After that,
press “Pause” button to continue.
STEP2: The 9600 LH will eject spin tips in the left half part of deep well plate.

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5.4 Instructions of keys
File:
New: Create a new file
Open: Open an old file
Save: Save a file
End: Close the file
Edit:
Cut: You can cut a whole row of data when you
select a certain row. You can also cut data of
selected cells.
Copy: Copy data of a whole row
Paste: Paste copied data
Move up: Move up a whole row of data
Move down: Move down a whole row of data
Insert: Insert a row of blank space
Delete: You can delete a whole row of data when
you select a certain row. You can also delete data
of selected cells.
COM Port：
Choose a COM port. There 16 COM ports.
COM1 is a preset port.
Assign a new COM port with the drop-down menu.

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5.5 Transmission of programs between the PC and M9600 LH
controller
 During program transmission, please do not switch off the machine and
the PC.
 Import: Transfer the program from the machine to the PC (A).
 Export: Transfer the programs from the PC to the machine (B).
(A) Example of program transmission (import):
There are 3 programs installed in the controller. You can use “AutoMag” to
retrieve these programs.
- Type “0” and press import button to get the program #0 (SHORTTEST)
from the controller. The details of the program will appear if the connection
between the PC and the controller is established.
- Type “1” and press import button to get the program #1 (EJECT-TIP) from
the controller.