Teledyne 5800 Operating instructions


Foreword
This instruction manual is designed to help you gain a thorough understanding of the operation of
the equipment. Teledyne Isco recommends that you read this manual completely before placing the
equipment in service.
Although Teledyne Isco designs reliability into all equipment, there is always the possibility of a
malfunction. This manual may help in diagnosing and repairing the malfunction.
If a problem persists, call or e-mail Teledyne Isco technical support for assistance. Simple
difficulties can often be diagnosed over the phone. For faster service, please have your serial
number ready.
If it is necessary to return the equipment to the factory for service, please follow the shipping
instructions provided by technical support, including the use of the Return Material
Authorization (RMA) specified. Be sure to include a note describing the malfunction. This will
aid in the prompt repair and return of the equipment.
Teledyne Isco welcomes suggestions that would improve the information presented in this manual
or enhance the operation of the equipment itself.
Teledyne Isco is continually improving its products and reserves the right to change product
specifications, replacement parts, schematics, and instructions without notice.
Contact Information
Customer Service
Phone: (800) 228-4373 (USA, Canada, Mexico)
(402) 464-0231 (Outside North America)
Fax: (402) 465-3022
Email: [email protected]
Technical Support
Phone: Toll Free (800) 775-2965 (Syringe Pumps and Liquid Chromatography)
Email: [email protected]
Return equipment to: 4700 Superior Street, Lincoln, NE 68504-1398
Other Correspondence
Mail to: P.O. Box 82531, Lincoln, NE 68501-2531
Email: [email protected]


Isco 5800 Refrigerated Sampler
v
Isco 5800 Refrigerated Sampler
Safety Information The Isco 5800 Refrigerated Sampler is a “definite purpose”
device, intended for use only with compatible Isco equipment.
Except as described in this manual, do not use this product with
any other manufacturers’ equipment, or for any other purpose.
Use for any purpose not described in this manual could cause
personal injury or property damage.
Electrical Requirements The sampler requires either 100 VAC/50 Hz, 115 VAC/60 Hz, or
230 VAC/50 Hz AC power. See , on page 20. Refer to the
nameplate and ratings label inside the refrigerator
compartment. The power input is through the mains power cord
at the back of the sampler. The sampler’s compressor is protected
by an internal thermal cut-out. Additionally, the sampler
controller circuitry is protected by an internal 5.0 ampere PTC
(Positive Temperature Coefficient) device.
WARNING
Electrocution hazard. Never manipulate electrical switches
or power connections with wet hands or when your feet are
in contact with water.
WARNING
AC electrical power must meet the applicable electrical
code requirements for your installation and must be
provided with an earth ground connection. If necessary,
consult with a certified electrician to ensure that AC power
is provided in accordance with the local electrical code.
The AC power cord of this device is equipped with a three-prong
grounding plug designed to mate with a grounded power outlet.
Grounding minimizes the possibility of electrical shock.
It is the user’s responsibility to ensure that the AC power source
is properly grounded. If in doubt, have the outlet checked by a
qualified electrician.
If the available AC power outlet only accepts two prongs, or if it
is determined that the outlet is improperly grounded, the outlet
must be replaced by a qualified electrician before attempting to
power this device.
WARNING
Never modify the AC power cord or use a three-prong to
two-prong adapter.
WARNING
If this device’s power cord is frayed or otherwise damaged,
discontinue its use immediately. The AC power cord is not
user-serviceable and must be returned to the factory for
repair or replacement.

Isco 5800 Refrigerated Sampler
vi
General Warnings Before installing, operating, or maintaining this equipment, it is
imperative that all hazards and preventive measures are fully
understood. While specific hazards may vary according to
location and application, take heed in the following general
warnings:
WARNING
This instrument has not been certified for use in
“hazardous locations” as defined by the National Electrical
Code.
WARNING
Avoid hazardous practices! If you use this instrument in
any way not specified in this manual, the protection
provided by the instrument may be impaired; this will
increase your risk of injury.
WARNING
Servicing of this product without proper training and
qualifications can potentially expose you to electrical and
mechanical hazards that can result in serious or fatal injury.
Servicing must be performed only by trained and qualified
personnel.
WARNING
Removing a module exposes you to electrical and
mechanical hazards. Always disconnect from AC power
before attempting to remove any module. Only trained
service personnel may remove or replace these modules.
WARNING
The sampler has a safety interlock that prevents the pump
from operating when the pump housing band is open. The
pump is extremely powerful and can injure you severely if
the sampler activates the pump during
maintenance/servicing. Remove power from the sampler
before opening the pump housing.
Additional safety information can be found in Appendix B.

Isco 5800 Refrigerated Sampler
vii
Hazard Severity Levels This manual applies Hazard Severity Levels to the safety alerts,
These three levels are described in the sample alerts below.
CAUTION
Cautions identify a potential hazard, which if not avoided, may
result in minor or moderate injury. This category can also warn
you of unsafe practices, or conditions that may cause property
damage.
WARNING
Warnings identify a potentially hazardous condition, which
if not avoided, could result in death or serious injury.
DANGER
DANGER – limited to the most extreme situations
to identify an imminent hazard, which if not
avoided, will result in death or serious injury.

Isco 5800 Refrigerated Sampler
viii
Hazard Symbols The equipment and this manual use symbols used to warn of
hazards. The symbols are explained below.
Hazard Symbols
Warnings and Cautions
The exclamation point within the triangle is a warning sign alerting you of
important instructions in the instrument’s technical reference manual.
The lightning flash and arrowhead within the triangle is a warning sign alert-
ing you of “dangerous voltage” inside the product.
Pinch point. These symbols warn you that your fingers or hands will be seri-
ously injured if you place them between the moving parts of the mechanism
near these symbols.
Symboles de sécurité
Ce symbole signale l’existence d’instructions importantes relatives au
produit dans ce manuel.
Ce symbole signale la présence d’un danger d’électocution.
Risque de pincement. Ces symboles vous avertit que les mains ou les
doigts seront blessés sérieusement si vous les mettez entre les éléments
en mouvement du mécanisme près de ces symboles
Warnungen und Vorsichtshinweise
Das Ausrufezeichen in Dreieck ist ein Warnzeichen, das Sie darauf
aufmerksam macht, daß wichtige Anleitungen zu diesem Handbuch
gehören.
Der gepfeilte Blitz im Dreieck ist ein Warnzeichen, das Sei vor “gefährlichen
Spannungen” im Inneren des Produkts warnt.
Vorsicht Quetschgefahr! Dieses Symbol warnt vor einer unmittelbar drohen-
den Verletzungsgefahr für Finger und Hände, wenn diese zwischen die
beweglichen Teile des gekennzeichneten Gerätes geraten.
Advertencias y Precauciones
Esta señal le advierte sobre la importancia de las instrucciones del manual
que acompañan a este producto.
Esta señal alerta sobre la presencia de alto voltaje en el interior del pro-
ducto.
Punto del machacamiento. Sus dedos o manos seriusly serán dañados si
usted los coloca entre las piezas móviles cerca de estos símbolos.

ix
Isco 5800
Refrigerated Sampler
Table of Contents
Section 1 Introduction
1.1 Sampler Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.2 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.3 Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Section 2 Installation
2.1 Unpacking the Sampler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.2 5800 Sampler Installation Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.3 Positioning the Sampler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.4 Connecting Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
2.4.1 Backup Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
2.5 Installing a Bottle Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
2.5.1 Installing the Distributor Arm and Discharge Tube . . . . . . . . . . . . . . . . . . 2-7
2.5.2 24 Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8
2.5.3 4 10L (2.5 Gal) Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11
2.5.4 2 Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11
2.5.5 4, 20L (5 Gal) Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-12
2.5.6 1 Bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-12
2.6 Attaching the Suction Line. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
2.6.1 Vinyl Suction Line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
2.6.2 FEP-lined Suction Line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-15
2.7 Attaching a Strainer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16
2.7.1 Alternative to Strainers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-17
2.7.2 Maximum Unanchored Length . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-17
2.8 Routing the Suction Line and Strainer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-17
2.9 Connecting the Sampler to External Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-18
2.9.1 Standard Isco Instrument Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-18
2.9.2 Other Device Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-19
2.10 Configuring and Programming the Sampler . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-21
2.11 Locking the Sampler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-21
Section 3 Programming
3.1 Control Panel Description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.2 Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.3 Configuring the Sampler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
3.3.1 Set Clock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
3.3.2 Bottles and Sizes-Standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
3.3.3 Suction Line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
3.3.4 Liquid Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
3.3.5 Flow Mode Sampling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
3.3.6 Enable Pin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
3.3.7 Refrigeration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
3.3.8 Output Pins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
3.3.9 Tubing Life . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8

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3.3.10 Program Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
3.3.11 Language . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
3.3.12 System IDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
3.3.13 Run Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
3.3.14 Exit Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
3.4 Sampling Program Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
3.5 Programming Steps. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-15
3.5.1 Program Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-15
3.5.2 Pacing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-15
3.5.3 Sample Every
__ Hours, __ Minutes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-16
3.5.4 Sample Every
__ Pulses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-16
3.5.5 __ Bottles per Sample Event . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-18
3.5.6 Switch on Time or Number of Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-18
3.5.7 Switch Bottles/Sets Every __ Hours, __ Minutes . . . . . . . . . . . . . . . . . . . . 3-19
3.5.8 First Switch Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-19
3.5.9 __Samples per Bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-19
3.5.10 Run Continuously? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-19
3.5.11 Enter Sample Volume Use Defined Sample . . . . . . . . . . . . . . . . . . . . . . . 3-20
3.5.12 Volumes Dependent on Flow? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-20
3.5.13 Sample Volume __ ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-20
3.5.14 Flow Pulses,
Analog Input . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-20
3.5.15 10 ml for Every
__ Pulses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-21
3.5.16 Sample Volume at
20 mA: __ ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-21
3.5.17 __ Composite Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-21
3.5.18 Suction Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-21
3.5.19 Pause & Resume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-22
3.5.20 No Delay to Start
Set Start Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-23
3.5.21 Auto Re-Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-24
3.5.22 First Sample At: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-24
3.5.23 Start Flow Count At: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-24
3.5.24 Select Days: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-24
3.5.25 First Valid Day . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-24
3.5.26 Maximum Run Time
__ Hours . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-24
3.6 Programming Examples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-24
Section 4 Operation
4.1 Start a Sampler Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.1.1 Start Time Delay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.1.2 The Run State . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
4.1.3 Completed Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
4.2 Pause or Stop a Running Program. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
4.3 Post Sampling Activities. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
4.3.1 View the Log . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
4.3.2 Retrieve the Temperature Log . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
4.3.3 Sampling Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
4.3.4 Remove Sample Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
4.4 Grab Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8
4.5 Calibrate Sample Volumes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9
4.6 Operate the Pump Manually . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-10

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Section 5 Maintenance
5.1 Periodic Maintenance Checklist. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.1.1 Pump Inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.1.2 Pump Tube Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
5.1.3 Cleaning the Pump Rollers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
5.1.4 Cleaning the Pump Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
5.1.5 Cleaning Wetted Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
5.1.6 Distributor Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
5.1.7 Sampler Cleaning Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
5.2 Sampler Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7
5.2.1 Test ‘RAM’ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7
5.2.2 Test ‘ROM’ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8
5.2.3 Test Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8
5.2.4 Test Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8
5.2.5 Test Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-9
5.2.6 Test Distributor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-9
5.2.7 Test Flow Meter Port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-10
5.2.8 Refrig Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-10
5.2.9 PCB Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-11
5.2.10 Input Voltage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-11
5.2.11 Battery Voltage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-11
5.2.12 Loaded Battery Volts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-12
5.2.13 Analog Input . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-13
5.3 Temperature Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-14
Appendix A Replacement Parts List
A.1 5800 Refrigerated Sampler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-2
A.2 Bottle Configurations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-32
Appendix B General Safety Procedures
B.1 Hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-1
B.2 Planning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-2
B.3 Adverse Atmospheres . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-2
B.4 Entering Manholes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-2
B.4.1 Traffic Protection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-3
B.4.2 Removing the Covers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-3
B.4.3 Other Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-3
B.4.4 Emergencies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-4
B.4.5 Field Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-4
B.5 Lethal Atmospheres in Sewers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-4
List of Figures
1-1 5800 Sampler features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
2-1 Minimum space requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
2-2 Installing the distributor arm (A) and discharge tube (B) . . . . . . . . . . . . . . . . . . . 2-5
2-3 Insert the keyed end first so that it faces the back of the
refrigerated compartment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
2-4 The 24-bottle rack is held in place by a latch on the left side . . . . . . . . . . . . . . . . 2-7
2-5 Installing the standard locating base . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8
2-6 Four-bottle, 20L locating base: Bottle mouth placement . . . . . . . . . . . . . . . . . . . . 2-9
2-7 One 20 liter bottle configuration with discharge tube through the bottle cap . . 2-10
2-8 Attaching the suction line to the pump tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-12
2-9 Connecting FEP-lined suction line (A) to pump tube (B) . . . . . . . . . . . . . . . . . . . 2-12

Isco 5800 Refrigerated Sampler
Table of Contents
xii
2-10 Strainers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
2-11 External device connection at rear of sampler . . . . . . . . . . . . . . . . . . . . . . . . . . 2-15
2-12 5800 Sampler to Isco flow meter cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16
2-13 5800 Sampler unterminated connect cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16
2-14 Quick disconnect box 60-2004-228 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-17
2-15 5800 battery backup module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-19
3-1 Control panel and keypad buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3-2 Suction line length . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
3-3 Time paced sampling program flow chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13
3-4 Flow paced sampling program flow chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-14
3-5 Suction Head Height . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-21
3-6 Example screens for Pause & Resume option . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-22
4-1 5800 Sampler to RS-232 serial communication cable (DB9-pin version shown) . 4-6
4-2 Sample temperature report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
4-3 Sliding the 24-bottle rack out . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8
5-1 5800 Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
5-2 Rollers (A) and guides (B) on the pump rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
5-3 Wetted Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
5-4 Temperature verification with the 24-bottle rack . . . . . . . . . . . . . . . . . . . . . . . . 5-13
5-5 Temperature verification with the bottle locating base . . . . . . . . . . . . . . . . . . . . 5-13
List of Tables
1-1 5800 Sampler Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
1-2 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
2-1 Bottle Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
2-2 Strainers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
2-3 Unterminated Connect Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-17
3-1 Control Button Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3-2 Flow Pulse Intervals at Various
Input Currents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-16
4-1 5800 Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
5-1 Replacement Pump Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
B-1 Hazardous Gases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-6
List of Examples
Defining the Sample Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-23
Time Paced Sampling Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-24
Flow Paced Sampling Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-26
Flow-proportional Constant Time Variable Volume Program. . . . . . . . . . . . . . . . . . . 3-28
Resetting the Pump Tube Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5

1-1
Isco 5800
Refrigerated Sampler
Section 1 Introduction
In this section…
•Sampler Overview
•Features, page 1-2
•Specifications, page 1-4
1.1 Sampler Overview The 5800 Refrigerated Sampler is an automated wastewater
sampler that can collect liquid samples from a variety of sources
including open channels, sewers, and storm pipe conduits.
This indoor/outdoor use sampler is made of durable UV-resistant
plastic that provides exceptional resistance to corrosion and
weathering. Most notably, the refrigerator body is made from
rotationally molded linear low-density polyethylene. This unique
construction material was selected for its strength, durability,
and its ability to withstand temperature extremes. The system is
rated for –29 to 49 °C (–20 to 120 °F) operation. The hollow
cavities are filled with foam insulation. To protect the non-CFC
system, all refrigeration parts are powder coated with
heat-treated polyester.
Unattended sample collection events can be triggered by time
intervals (time pacing), or by flow volume measured by an
external flow measurement device (flow pacing or constant
volume variable time). Isco flow meters are compatible flow
measurement devices, as well as any device that can provide a 5
to 15 VDC pulse or contact closure of at least 25 mS duration, or
a linear 4-20 mA current loop signal. An external device can also
disable the sampler until measured site conditions are met, at
which time the sampler begins the automated collection.
The sampler’s pump can deliver the liquid through a 9 mm (3/8")
suction line at transport velocities of at least 0.9 meters per
second (3.0 ft/sec) at 0.9 m (3.0 ft) of head. The transport velocity
at 7.6 m (25 ft) of head is 0.67 m/sec (2.2 ft/sec). The maximum
lifting height of the sampler is 8 m (28 ft). To produce accurate
and repeatable samples, the pump uses a pump revolution
counter and non-contacting liquid detector.
The collected samples are stored inside the refrigerated
compartment and cooled for sample preservation. Samples can
be deposited into a single bottle (composite), or collected as
discrete samples, each into a separate bottle of a multi-bottle
rack (sequential), or a combination of these modes. The cooled
samples are maintained until an operator can remove them from
the sampler and deliver them to the laboratory.

Isco 5800 Refrigerated Sampler
Section 1 Introduction
1-3
Table 1-1 5800 Sampler Features
Item
(Fig. 1-1) Name Description
1 Control Panel Cover Protects the control panel display and keypad. Can be locked with user
supplied padlock.
2 Control Panel Supports user control and programming of sampler operation.
3 Discharge Tube Carries liquid to the sample bottle. For 24, 4, 2, and 10 liter one-bottle
configurations, the discharge tube is routed through the distributor arm.
For the 20 liter one-bottle configuration the discharge tube is routed
directly into the bottle.
4 Distributor Arm Directs collected liquids to the bottle. Movement of the distributor is con-
trolled by user-specified program settings.
5 Bottles Hold the collected samples.
6 Bottle Locator Rack Positions the bottles under the distributor.
7 Refrigerator Door Protects the collected samples inside the refrigerated compartment.
8 Latch Secures the door. The latch mechanism may be locked with a user-sup-
plied padlock.
9 Level Adjustment Feet The sampler includes two level adjustment feet at the front of the sam-
pler. Use these to keep the bottle rack or locating base level inside the
refrigerated compartment.
10 Liquid Detector and Pump
(cover removed)
The non-wetted liquid detector senses when the liquid is at the pump to
determine head height and sample volumes. The peristaltic pump col-
lects sample liquid and performs suction line rinses.
11 Lift Points (2 upper, 2 lower,
each side)
If you must lift the sampler, it can be safely handled at these points. Lift-
ing must be done by two people, one on each side, with the door closed
and latched.
12 External Device Connection Supports connections to external devices such as a flow meter for sam-
ple pacing and enable signals, and connection to a personal computer for
data collection.
13 Refrigeration Assembly Modular, corrosion-resistant refrigeration assembly cools the refrigerated
compartment to a user-selected temperature of 1 to 9°C (34 to 48°F).
14 AC Mains Line Cord Connects to AC power source.
The Mains Line Cord is the disconnect device.

Isco 5800 Refrigerated Sampler
Section 1 Introduction
1-4
1.3 Specifications
Table 1-2 Specifications
Size (HWD): 132 73 84 cm 52.0 28.8 33.2 in
Weight (empty): 88.5 kg 195 lb
Bottle Configurations: 13 configurations available:
24, 1-liter PP
24, 350-ml glass
4, 10-liter PE or glass
4, 20-liter PE
2, 10-liter PE or glass
1, 20-liter PE or glass
1, 10-liter PE or glass
24 ProPaks, 1-liter wedge
1 ProPak, 10-liter round
Refrigerator Body: Linear low-density polyethylene (LLDPE)
Power Requirements: 115 VAC ±10%, 60 Hz Sampler
Running current 4.0 amperes
230 VAC ±10%, 50 Hz Sampler
Running current 2.0 amperes
Installation Category: II
Pollution Degree: 3
Maximum Altitudea: 2,000 meters 6,562 feet
Humidity: 0 to 100%
Operational Temperature:b–29 to 49 °C –20 to 120 °F
Pump
Intake Suction Tubing Length: 1 to 30 m 3 to 99 feet
Material: Vinyl or FEP-lined polyethylene
Inside Diameter: 9 mm 3/8inch
Pump Tubing Life: Typically 1,000,000 pump counts.
Maximum Suction Lift: 8.5 m 28 feet
Typical Repeatability: ±5ml or ±5% of the average volume in a set, whichever is greater, at lifts up to
25 feet.
Typical Delivered Volume Accuracy
(Ability to repeat the delivered volume
for a set of samples collected under
the same conditions.):
±10ml or ±10% of programmed value, whichever is greater, at lifts up to 25
feet.
Typical Line Velocity at Head Height: 0.9 m: 0.91 m/s 3 ft: 3.0 ft/s
3.1 m: 0.87 m/s 10 ft: 2.9 ft/s
4.6 m: 0.83 m/s 15 ft: 2.7 ft/s
7.6 m: 0.67 m/s 25 ft: 2.2 ft/s
Liquid Presence Detector: Non-wetted, non-conductive sensor detects when liquid sample reaches the
pump to automatically compensate for changes in head heights.
Controller
Enclosure Rating: IP67 NEMA 4X, 6
Program Memory: Non-volatile ROM (Flash)

Isco 5800 Refrigerated Sampler
Section 1 Introduction
1-5
Flow Meter Signal Inputs: 5 to 15 volt DC pulse or 25 millisecond isolated contact closure for Isco flow
meters. 4-20 mA input for non-Isco flow meters.
Digital Alarms: 4 programmable outputs; 5 VDC, 100 mA
Number of Composite Samples: Programmable from 1 to 999 samples.
Software
Sample Frequency: 1 minute to 99 hours 59 minutes, in 1-minute increments. 1 to 9,999 flow
pulses.
Sampling Modes: • Constant Time, Constant Volume
• Variable Time, Constant Volume
• Constant Time, Variable Volume
(Variable time and variable volume modes are controlled by an external flow
meter signal)
Programmable Sample Volumes: 10 to 9,990 ml in 1 ml increments.
Sample Retries: If no sample is detected, up to 3 attempts; user selectable.
Rinse Cycles: Automatic rinsing of suction line up to 3 rinses for each sample collection.
Controller Diagnostics: Tests for RAM, ROM, pump, display, and electrical components.
Refrigeration
Temperature Accuracy ±1° C from average temperature over a 48 hour period.
Bottle configuration: 1 X 2.5 gal. polyethylene. Tested with setpoint 3°C, ambi-
ent temperature 20°C, in 30ml glycol bottle (not included) located on rack
between rear rack locations 3 and 4 (see figure below).
a. The maximum altitude rating is per European Norm 61010-1, which establishes safety requirements for electrical equip-
ment. The rating pertains to electrical creepage and clearances. The rating is not applicable to pump performance.
b. The display may take longer to show information at temperatures below 0°F.
Table 1-2 Specifications (Continued)

Isco 5800 Refrigerated Sampler
Section 1 Introduction
1-6

2-1
Isco 5800
Refrigerated Sampler
Section 2 Installation
In this section…
• Unpacking the Sampler
• 5800 Sampler Installation Instructions
2.1 Unpacking the
Sampler
Carefully unpack the shipment and locate the packing list. Verify
receipt of all components against this packing list. If there is any
damage to the shipping carton or any components, contact the
shipping agent and Teledyne Isco (or its authorized
representative) immediately.
WARNING
If there is any evidence that the sampler has been damaged
in shipping, do not plug it into an AC power source. Contact
Teledyne Isco or its authorized representative for advice.
Compare the contents of the boxes with the enclosed
packing slip. If there are any shortages, contact Teledyne
Isco immediately.
2.2 5800 Sampler
Installation Overview
The following sections provide general instructions for placing
the sampler into operation. In typical applications, the steps are:
1. Position the sampler. (Section 2.3)
2. Connect power. (2.4)
3. Install a bottle kit. (2.5)
4. Attach the suction line. (2.6)
5. Attach a strainer. (2.7)
6. Route the suction line and strainer. (2.8)
7. Connect external instruments. (2.9)
8. Configure and program the sampler. (2.10)
9. Lock the sampler. (2.11)
2.3 Positioning the
Sampler
There are a few considerations when selecting a site for the
sampler. The foremost concern should be personal safety.
WARNING
The installation and use of this product may subject you to
dangerous working conditions that can cause you serious
or fatal injuries. Take any necessary precautions before
entering the worksite. Install and operate this product in
accordance with all applicable safety and health
regulations, and local ordinances.

Isco 5800 Refrigerated Sampler
Section 2 Installation
2-2
WARNING
If this product is used in a manner not specified in this
manual, the protection provided by the equipment may be
impaired.
WARNING
This product has not been approved for use in hazardous
locations as defined by the National Electrical Code.
WARNING
The sampler is heavy. When lifting, use a two-person lift at
the points identified in Table 1-1. When possible, move the
sampler using a two-wheeled hand cart from the back. To
prevent damage to the refrigeration system, do not tip the
sampler more than 45°.
•Dangerous locations – If you must enter a manhole,
confined space, or other dangerous location to install the
suction line and strainer, observe standard safety
precautions. Refer to Appendix B for a discussion of
safety considerations.
•Support – The sampler should be installed on a surface
capable of safely supporting the sampler, full liquid
containers, and personnel.
•AC Power – The mains line cord is the disconnect device
should you need to remove power. Therefore, the
electrical power outlet should be accessible.
•Environmental – The sampler is designed for outdoor
use. Refer to Table 1-2 for environmental specifications.
When possible, avoid subjecting the sampler to chemical
attacks and direct sunlight.
•Avoid submersion – Although the control panel will
resist damage (rated IP 67; NEMA 4x, 6), the
refrigerator system and bottle compartment cannot
prevent liquid from entering. In the event of
submersion, liquid entering the refrigeration system
could permanently damage the sampler; liquid entering
the bottle compartment could contaminate the collected
samples. Liquid entering the electrical compartment for
the refrigeration system could result in a short circuit
and possible shock hazard.
•Liquid sample collection point – It is best to keep the
distance between the sampler pump and the collection
point as short as possible. The shorter distance will
increase pumping efficiency and pump tubing life. Also,
the pump inlet should be located above the liquid
surface to be sampled with no liquid traps. Gravity will
aid suction line rinses and allow the line to drain,
Other manuals for 5800
4
Table of contents
Other Teledyne Refrigerator manuals