Thermo Scientific Multidrop Combi nL User manual

Thermo Scientific
Multidrop Combi nL
User Manual
Rev. 1.2, Cat. no. N07171

Copyright
Copyright 2019 Thermo Fisher Scientific Inc. All rights reserved. First edition published in 2007.
Reproduction of the accompanying user documentation in whole or in part is prohibited.
Trademarks
“FILLit” is a trademark and “Microtiter”, “Multidrop” and “Nalgene” are registered trademarks of Thermo
Fisher Scientific Inc. and its subsidiaries.
“Decon” is a registered trademark of Decon Laboratories Limited.
“Micro-90” is a registered trademark of International Products Corp.
“Microside SQ” is a registered trademark of Global Biotechnologies, Inc.
“Teflon” and “Virkon” are registered trademarks of E.I. du Pont de Nemours and Company or its affiliates.
All other trademarks and registered trademarks are the property of their respective holders.
Disclaimer
Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate
technological developments. This manual is subject to change without prior notice as part of continuous
product development. Although this manual has been prepared with every precaution to ensure accuracy,
Thermo Fisher Scientific assumes no liability for any errors or omissions, nor for any damages resulting from
the application or use of this information. This manual supersedes all previous editions.
Remarks on screenshots
Screenshots may be slightly different on your system depending on the firmware version.
No liability for consequential damages
Thermo Fisher Scientific shall not be liable for any indirect or consequential damages whatsoever arising out of
the use or inability to use this product.
Power failure
The system requires uninterrupted power supply in order to operate correctly. Thermo Fisher Scientific has no
responsibility whatsoever for system malfunctions arising from power failures.
Manufacturer
Life Technologies Holdings Pte. Ltd.
(a part of Thermo Fisher Scientific Inc.)
33, Marsiling Industrial Estate Road 3, #7-06,
Singapore 739256

Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 3
About This User Manual
This user manual is written for the actual end user, for example,
research scientist or laboratory technician, and provides information on
the Thermo Scientific Multidrop Combi nL, including the installation
and operating instructions.
This user manual is designed to give you the information to:
lReview safety precautions
lSet up the Multidrop Combi nL
lUse the Multidrop Combi nL internal software
lCarry out dispensing procedures
lPerform basic cleaning and maintenance procedures
lTroubleshoot the instrument performance
This user manual also describes all the features and specifications of the
Multidrop Combi nL instrument as well as ordering information.
Read the manual in its entirety before operating the instrument.
Keep the user manual for future reference. The user manual is an
important part of the instrument and should be readily available.
For PC software-related issues, refer to the Thermo Scientific FILLit
Software for Multidrop Combi nL User Manual (Cat. no. N17562).
For the latest information on products and services, visit our websites
at:
http://www.thermoscientific.com
http://www.thermoscientific.com/multidrop
In our efforts to provide useful and appropriate documentation, we
would appreciate any comments you may have on this user manual to
your local Thermo Fisher Scientific representative.
Intended users
How to use this
user manual
For more
information

About This User Manual
Safety symbols and markings
4Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific
These symbols are intended to draw your attention to particularly
important information and alert you to the presence of hazards as
indicated.
The following symbols and markings appear on the type label and the
instrument itself.
Power ON s
Power OFF s
Serial number s
Catalog number s
Date of manufacture s
Consult instructions for use s
WEEE symbol This product is required to comply with the European
Union’s Waste Electrical & Electronic Equipment (WEEE) Directive
2012/19/EU. s
Safety symbols
and markings
Safety symbols and
markings
used on the
Multidrop Combi nL

About This User Manual
Safety symbols and markings
Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 5
The following symbols and markings appear in this user manual.
Warning Risk of electric shock. s
Warning Biohazard risk. s
Warning Risk of injury to the user(s). s
Caution Risk of damage to the instrument, other equipment or loss of
performance or function in a specific application. s
Note Marks a hint, important information that is useful in the
optimum operation of the system, or an item of interest. s
Warning and other
markings used in
the documentation

About This User Manual
Instrument safety and guidelines for use
6Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific
lAlways follow basic safety precautions when using the Multidrop
Combi nL to reduce the risk of injury, biohazardous
contamination, fire, or electric shock.
lRead this user manual in its entirety prior to operating the
instrument. Failure to read, understand, and follow the instructions
in the manual may result in damage to the instrument, injury to
laboratory and operating personnel or poor instrument
performance.
lObserve all “Warning”, “Caution”, and “Note” statements as well as
safety symbols and markings on the instrument and in the
documentation.
lThe Multidrop Combi nL is intended for laboratory research use
only. Observe proper laboratory safety precautions, such as wearing
protective clothing and following approved laboratory safety
procedures.
lUse of the Multidrop Combi nL in ways other than those described
in the documentation supplied with the equipment may result in
injury to persons or damage to the property. Avoid unintended use
of the equipment, for example, using incompatible materials,
making unauthorized modifications, using incompatible or
damaged parts, using unapproved auxiliary equipment or
accessories, or operating equipment in excess of maximum ratings.
lDo not touch the dispensing valves or the tubing inlet to ensure
trouble-free dispensing. Thermo Fisher Scientific assumes no
liability for the use of third-party dispensing valves.
lPreventative maintenance instructions should be followed closely to
keep the instrument in the best condition for maximum reliability.
A poorly maintained instrument will not give the best results.
Instrument safety
and guidelines
for use

Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 7
Contents
Intended users...................................................................................3
How to use this user manual ............................................................. 3
For more information .......................................................................3
Safety symbols and markings.............................................................4
Safety symbols and markings used on the Multidrop Combi nL .... 4
Warning and other markings used in the documentation...............5
Instrument safety and guidelines for use............................................ 6
Introduction to the MultidropCombi nL................................................ 12
Intended use.................................................................................... 13
Principle of operation......................................................................13
Pressure/vacuum system ............................................................... 13
Dispensing applications................................................................13
Calibrations..................................................................................14
Advantages of using Multidrop Combi nL ...................................... 14
Functional Description ............................................................................... 15
Instrument layout............................................................................15
Front view.................................................................................... 15
Back view ..................................................................................... 16
Installation .................................................................................................... 17
Delivery check................................................................................. 17
Checking delivery......................................................................... 17
Unpacking ...................................................................................17
Requirements.................................................................................. 18
Precautions and limitations .............................................................18
Installation setups............................................................................18
Releasing the transport lock..........................................................19
Installing the priming vessel ......................................................... 20
Installing the pressure/vacuum system reservoir............................ 20
Operational check ...........................................................................23
Routine Operation........................................................................................ 26
Dispensing valves ............................................................................26
Calibration................................................................................... 27
Reagent reservoir............................................................................. 28
Control panel.................................................................................. 29
Keyboard...................................................................................... 29
Keys .......................................................................................... 29
Display.........................................................................................30
Navigating ................................................................................... 31
Dispensing ...................................................................................... 33
Chapter 1
Chapter 2
Chapter 3
Chapter 4

Contents
8Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific
Using FILLit PC software ...............................................................34
Using internal software....................................................................34
Dispensing parameters ....................................................................34
Plate type .....................................................................................34
Dispensing volume and liquid...................................................... 36
Column/Row selection.................................................................37
Full plate...................................................................................37
Column selection ......................................................................38
Setting the column volume .......................................................38
Row selection............................................................................40
Setting the row volume .............................................................40
Selecting columns and rows (Selected wells)..............................41
Protocol settings.............................................................................. 42
Dispensing speed..........................................................................43
Shaking ........................................................................................ 44
Dispensing offsets.........................................................................45
Height.......................................................................................46
X/Y offset.................................................................................. 47
Predispensing volume................................................................... 47
Dispensing direction .................................................................... 48
Running a protocol .........................................................................50
Save and edit protocol..................................................................51
Select protocol..............................................................................54
Delete protocol ............................................................................54
Instrument options..........................................................................56
Device information ......................................................................56
Computer interface ......................................................................57
Startup protocol ...........................................................................58
Instrument settings.......................................................................59
Calibration................................................................................... 60
Liquid factor (standalone calibration)........................................ 60
Accuracy check..........................................................................61
Factor calculation...................................................................... 63
Screen saver..................................................................................... 65
Shutdown........................................................................................65
Emergency situations ......................................................................66
Maintenance .................................................................................................67
Maintenance checklist..................................................................... 67
Regular and preventive maintenance ...............................................68
How to clean the tubings .............................................................69
Daily maintenance ....................................................................69
Removing foreign particles........................................................69
Disinfecting the liquid path.......................................................72
Routine maintenance of the dispensing valves (tips).....................72
Daily maintenance ....................................................................72
Tip washing ..............................................................................73
Emptying ..................................................................................73
Periodic maintenance ................................................................73
Chapter 5

Contents
Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 9
How to clean a leaking dispensing valve.................................... 73
How to clean a clogged dispensing valve ................................... 75
Replacing a dispensing valve...................................................... 75
Replacing a tubing set and a manifold.......................................77
How to clean the reagent reservoir ...............................................78
How to clean the plate carrier....................................................... 79
How to clean the priming vessel................................................... 79
Disposal of materials .......................................................................80
Decontamination procedure............................................................80
How to refit the transport lock........................................................ 82
Packing for service........................................................................... 83
Service contracts.............................................................................. 84
Disposal of the instrument ..............................................................85
Technical Specifications ........................................................................... 86
General specifications...................................................................... 86
Safety specifications......................................................................... 87
Live parts .....................................................................................87
In conformity with the requirements............................................... 87
Remote control to Multidrop Combi nL......................................... 88
Frequently Asked Questions...................................................................... 89
Q&As ............................................................................................. 89
Troubleshooting Guide ............................................................................... 91
Error and warning codes .................................................................91
Hazards...........................................................................................93
Electrical ......................................................................................93
Mechanical................................................................................... 94
Environmental ............................................................................. 94
Defects and abnormal stresses.......................................................94
Ordering Information................................................................................... 95
Multidrop Combi nL......................................................................95
List of accessories ............................................................................ 95
Certificate of Decontamination................................................................. 97
Chapter 6
Chapter 7
Chapter 8
Chapter 9
Appendix A

Figures
10 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific
Figures
Figure 1–1. Multidrop Combi nL microplate dispenser .................................12
Figure 2–2. Multidrop Combi nL front view................................................... 15
Figure 2–3. Close-up of the Multidrop Combi nL keyboard and display.......16
Figure 2–4. Multidrop Combi nL back view...................................................16
Figure 3–5. Transport lock and transport lock tag present ...........................19
Figure 3–6. Removing the transport lock.......................................................19
Figure 3–7. Transport lock storage ................................................................20
Figure 3–8. Inserting the priming vessel .......................................................20
Figure 3–9. Removing the red luer plugs.......................................................21
Figure 3–10. Connecting the hosing to the pressure/vacuum system.......... 21
Figure 3–11. Connecting the hosing to the reagent reservoir.......................22
Figure 3–12. Close-up of the manifold ..........................................................22
Figure 3–13. Reagent reservoir connected to the instrument.......................23
Figure 3–14. Priming vessel inserted into place ...........................................24
Figure 4–15. Principle of the pressure/vacuum dispensing system .............26
Figure 4–16. Eight dispensing valves inserted into the manifold.................26
Figure 4–17. Reagent reservoir connected.................................................... 28
Figure 4–18. Removing the reagent filter......................................................29
Figure 4–19. Keyboard of the Multidrop Combi nL .......................................29
Figure 4–20. Main view on the display of the Multidrop Combi nL .............30
Figure 4–21. Example of dispensing with selected wells.............................42
Figure 4–22. Calibration curve vs. factor and volume changes ....................64
Figure 5–23. Removing the reagent filter......................................................70
Figure 5–24. Cleaning the tubings using a reagent basin.............................70
Figure 5–25. Detaching a dispensing valve tube end ...................................71
Figure 5–26. Reconnecting a dispensing valve tube end..............................72
Figure 5–27. Removing the reagent filter with tube .....................................74
Figure 5–28. Unfastening the dispensing valve ............................................76
Figure 5–29. Pulling the dispensing valve downwards.................................76
Figure 5–30. Replacing the dispensing valve ................................................ 77
Figure 5–31. Replacing the tubing set and manifold ....................................78
Figure 5–32. Unfastening the priming vessel................................................80
Figure 5–33. Transport lock storage location ................................................82
Figure 5–34. Refitting the transport lock.......................................................83
Figure 5–35. Transport lock fastened ............................................................83