Thermo Scientific Dionex UltiMate 3000 Series User manual

Thermo Scientific Dionex
UltiMate 3000 Series
LPG-3400XRS Pump
Operating Instructions
(Original Operating Instructions)
Revision: 1.1
Date: September 2013
© 2013 Thermo Fisher Scientific Inc.
Doc. No. 4820.4301

UltiMate 3000 Series:
LPG-3400XRS Pump
Operating Instructions

UltiMate 3000 Series:
LPG-3400XRS Pump
Operating Instructions Page I
Declaration of Conformity
(Original Declaration of Conformity)
Product: Thermo Scientific Dionex UltiMate 3000 - Pump
Types: ISO-3100SD, ISO-3100BM
HPG-3200SD, HPG-3200RS, HPG-3200BX
HPG-3400SD, HPG-3400RS
LPG-3400SD(N), LPG-3400RS, LPG-3400XRS
DGP-3600SD(N), DGP-3600RS
Dionex Softron GmbH herewith declares conformity of the above products with the
respective requirements of the following regulations:
•Low-Voltage Directive 2006/95/EC
•EMC Directive 2004/108/EC
The electrical safety of the products was evaluated based on the following standard:
•DIN EN 61010-1:2010
Safety requirements for electrical equipment for measurement, control and
laboratory use, Part 1: General Requirements
The Electromagnetic Compatibility (EMC) of the products was evaluated based on the
following standard:
•DIN EN 61326:2006
Electrical equipment for measurement, control and laboratory use
EMC Requirements
This declaration is issued for the manufacturer
Dionex Softron GmbH
Part of Thermo Fisher Scientific Inc.
Dornierstraße 4
D-82110 Germering
by the Managing Director, Rüdiger Obst, and
the Vice President HPLC, Fraser McLeod.
September 2, 2013

UltiMate 3000 Series:
LPG-3400XRS Pump
Page II Operating Instructions

UltiMate 3000 Series:
LPG-3400XRS Pump
Operating Instructions Page i
Table of Content
1Introduction................................................................................................................... 1
1.1 How to Use this Manual ............................................................................................ 1
1.2 Safety ......................................................................................................................... 3
1.2.1 Symbols on the Pump and in the Manual......................................................... 3
1.2.2 Safety Precautions ............................................................................................ 4
1.2.3 Consignes de Sécurité....................................................................................... 8
1.3 Intended Use ............................................................................................................ 12
1.4 Federal Communications Commission (FCC) Note................................................ 13
2Overview ...................................................................................................................... 15
2.1 Unit Description....................................................................................................... 15
2.2 Operating Principle.................................................................................................. 16
2.3 Functional Schematic............................................................................................... 17
2.4 Front Panel Elements............................................................................................... 18
2.5 Rear Panel ................................................................................................................ 19
2.5.1 Power Switch.................................................................................................. 20
2.5.2 Fuse Cartridge ................................................................................................ 20
2.5.3 USB Port......................................................................................................... 20
2.5.4 Digital I/O....................................................................................................... 20
2.6 Interior Components ................................................................................................ 21
2.7 Flow Path ................................................................................................................. 22
2.8 Fluid Connections.................................................................................................... 23
2.9 Rear Seal Wash System ........................................................................................... 24
2.10 Pump Head (Liquid Displacement Assembly) .................................................... 25
2.11 Mixing System..................................................................................................... 26
2.12 Purge Unit............................................................................................................ 27
2.13 Proportioning Valve............................................................................................. 27
2.14 Leak Sensor.......................................................................................................... 28
2.15 Vacuum Degasser ................................................................................................ 28
2.16 Operation with Chromeleon................................................................................. 29
2.17 Wellness and Predictive Performance ................................................................. 30
3Installation................................................................................................................... 31
3.1 Facility Requirements.............................................................................................. 31
3.2 Unpacking................................................................................................................ 32
3.3 Positioning the Pump in the UltiMate 3000 System................................................ 33
3.4 Connecting the Pump............................................................................................... 36
3.4.1 General Information ....................................................................................... 36
3.4.2 Connecting the USB Cable............................................................................. 36
3.4.3 Connecting the Power Cord............................................................................ 37
3.4.4 Connecting the Digital I/O ............................................................................. 37
3.5 Setting Up the Pump in Chromeleon ....................................................................... 45
3.5.1 Initial Installation............................................................................................ 45

UltiMate 3000 Series:
LPG-3400XRS Pump
Page ii Operating Instructions
3.5.2 Installing the Pump in Chromeleon ................................................................47
3.5.3 Configuring the Pump in Chromeleon............................................................48
3.6 Setting Up the Pump in DCMSLink ........................................................................52
3.7 When a Corona or Coulochem III Detector is connected ........................................53
4Preparation for Operation (Startup) ........................................................................55
4.1 Overview of Actions ................................................................................................55
4.2 General Precautions for Connecting and Handling Capillaries ...............................57
4.3 Solvent Reservoirs ...................................................................................................58
4.3.1 General Notes .................................................................................................59
4.3.2 Connecting the Solvent Reservoirs.................................................................60
4.4 Connecting Drain Tubing.........................................................................................62
4.5 Setting Up the Rear Seal Wash System ...................................................................62
4.6 Purging the Pump.....................................................................................................64
4.7 Equilibrating the System..........................................................................................66
5Operation and Maintenance ......................................................................................67
5.1 Power-Up .................................................................................................................67
5.2 Status Screen............................................................................................................68
5.3 Operation with Chromeleon.....................................................................................69
5.3.1 Connecting to Chromeleon.............................................................................69
5.3.2 Direct Control .................................................................................................70
5.3.3 Automated Control .........................................................................................72
5.4 Information for Operating the Pump........................................................................74
5.4.1 Choosing the Solvents ....................................................................................74
5.4.2 Using Pump Stroke Synchronization with the Autosampler ..........................76
5.4.3 Setting the Flow Rate, Flow Acceleration, and Flow Deceleration ...............81
5.4.4 Setting the Pressure Limits .............................................................................82
5.4.5 Recording the Pump Pressure.........................................................................82
5.4.6 Rear Seal Wash System ..................................................................................83
5.4.7 Purging the Pump ...........................................................................................84
5.4.8 Detecting Liquid Leaks in the Pump ..............................................................84
5.4.9 Adjusting the Screen Brightness or Contrast..................................................84
5.4.10 Vacuum Degasser ...........................................................................................85
5.5 Special Chromeleon Functions ................................................................................86
5.5.1 Predictive Performance...................................................................................86
5.5.2 Monitoring the Digital Inputs (Digital I/O)....................................................87
5.5.3 Operational Qualification and Performance Qualification .............................87
5.6 Shutting Down the Pump .........................................................................................88
5.7 Routine and Preventive Maintenance ......................................................................90
6Troubleshooting ..........................................................................................................93
6.1 Overview..................................................................................................................93
6.2 Error Codes on the Pump Display............................................................................94
6.3 Operating Problems..................................................................................................97

UltiMate 3000 Series:
LPG-3400XRS Pump
Operating Instructions Page iii
7Service ........................................................................................................................ 103
7.1 General Notes and Safety Precautions................................................................... 103
7.2 Eliminating Leakage.............................................................................................. 105
7.3 Pump Head, Pistons and Piston Seals.................................................................... 106
7.3.1 Visually Inspecting the Pump for Piston Seal Leakage................................ 109
7.3.2 Pump Head and Pistons................................................................................110
7.3.3 Replacing the Piston Seals............................................................................ 118
7.3.4 Replacing the Check Valve Cartridge .......................................................... 124
7.3.5 Replacing the Purge Unit.............................................................................. 125
7.3.6 Replacing the Proportioning Valve .............................................................. 126
7.4 Cleaning the Pump Head Components .................................................................. 127
7.4.1 Cleaning the Pistons ..................................................................................... 127
7.4.2 Cleaning the Check Valve Cartridges .......................................................... 128
7.5 Testing the Pump for Leakage ............................................................................... 129
7.6 Vacuum Degasser (Rinsing the Degassing Channels)........................................... 131
7.7 Replacing the Main Power Fuses........................................................................... 132
7.8 Updating the Pump Firmware................................................................................ 133
8Technical Information.............................................................................................. 135
9Accessories, Consumables, and Spare Parts .......................................................... 137
9.1 Standard Accessories ............................................................................................. 137
9.2 Optional Accessories ............................................................................................. 139
9.3 Consumables and Spare Parts ................................................................................ 140
10 Reference Information ............................................................................................. 143
10.1 Chemical Resistance of PEEK........................................................................... 143
10.2 Solvent Miscibility............................................................................................. 146
10.3 Properties of Common Solvents ........................................................................ 147
10.4 Safety Information about Flammable Solvents .................................................148
11 Appendix ─ Digital I/O (Pin Assignment) .............................................................. 151
12 Index........................................................................................................................... 155

UltiMate 3000 Series:
LPG-3400XRS Pump
Page iv Operating Instructions

UltiMate 3000 Series:
LPG-3400XRS Pump
Operating Instructions Page 1
1 Introduction
1.1 How to Use this Manual
The layout of this manual is designed to provide quick reference to the sections of interest
to the reader when operating the Thermo Scientific™ Dionex™ pump. However, in order
to obtain a full understanding of the pump, Thermo Fisher Scientific recommends that you
review the manual thoroughly before beginning operation.
The descriptions in this manual apply to the LPG-3400XRS pump of the UltiMate™ 3000
series.
The following conventions apply to the descriptions throughout this manual:
•The terms "the pump" or "the device" is used throughout the manual.
•If not otherwise stated, the descriptions for the Viper™ capillary connections apply also to
the nanoViper™ and possible other Viper capillary connections.
•The device configuration may vary. Therefore, not all descriptions necessarily apply to
your particular pump.
•It may happen that the representation of a component in this manual is different from the
real component. However, this does not influence the descriptions.
•The descriptions in this manual refer to firmware version 5.7 and Chromeleon™ 6.80
Service Release 13. If you want to operate the pump from Chromeleon 7, note the
information on page 29.
This manual is provided "as is". Every effort has been made to supply complete and
accurate information and all technical specifications have been developed with the utmost
care. The information contained in this manual should not be construed as a commitment
by Thermo Fisher Scientific. Thermo Fisher Scientific assumes no responsibility for any
errors that may appear in this document that is believed to be complete and accurate at the
time of publication and, in no event, shall Thermo Fisher Scientific be liable for incidental
or consequential damages in connection with or arising from the use of this document. We
appreciate your help in eliminating any errors that may appear in this document.
The information contained in this document is subject to change without notice.
All rights reserved, including those for photomechanical reproduction and storage on
electronic media. No part of this publication may be copied or distributed, transmitted,
transcribed, stored in a retrieval system, or transmitted into any human or computer
language, in any form or by any means, electronic, mechanical, magnetic, manual, or
otherwise, or disclosed to third parties without the express written permission of Thermo
Fisher Scientific Inc.

UltiMate 3000 Series:
LPG-3400XRS Pump
Page 2 Operating Instructions
Trademarks
Analyst is a registered trademark of AB Sciex.
Compass and Hystar are trademarks of Bruker Daltonics.
Luer-Lok is a trademark of Beckton, Dickinson and Company.
Empower is a trademark of Waters Corp.
PEEK is a trademark of Victrex PLC.
Microsoft and Windows are registered trademarks of Microsoft Corp.
All other trademarks are property of Thermo Fisher Scientific Inc. and its subsidiaries.

UltiMate 3000 Series:
LPG-3400XRS Pump
Operating Instructions Page 3
1.2 Safety
The CE Mark label and cTUVus Mark safety label on the rear panel indicate that the pump
is in compliance with the related standards.
1.2.1 Symbols on the Pump and in the Manual
The table shows the symbols used on the pump:
Symbol Description
Alternating current—Courant alternatif
Power supply is on (─)—Le module est mis sous tension (─) and Power supply
is off (O)—Le module est mis hors tension (O)
Refer to the Operating Instructions to prevent risk of harm to the operator and to
protect the instrument against damage.
Référez-vous à ce manuel pour éviter tout risque de blessure à l'opérateur et/ou
protéger l'instrument contre tout dommage.
Label according to the "Measures for Administration of the Pollution Control of
Electronic Information Products" (China RoHS) guideline
Étiquette "Measures for Administration of the Pollution Control of Electronic
Information Products" (China RoHS)
WEEE (Waste Electrical and Electronic Equipment) label—For more
information, see the WEEE Information section in the "Installation and
Qualification Documents for Chromatography Instruments" binder.
Étiquette DEEE (Déchets d'Equipements Electriques et Electroniques)—Pour
plus d'informations, référez-vous au chapitre WEEE Information dans le
classeur "Installation and Qualification Documents for Chromatography
Instruments"
At various points throughout the manual, messages of particular importance are indicated
by certain symbols:
Tip: Indicates general information, as well as information intended to
optimize the performance of the device.
Important: Indicates that failure to take note of the accompanying information
could cause wrong results or may result in damage to the device.
Important: Indique que ne pas tenir compte de l'information jointe peut conduire
à de faux résultat ou endommager l'instrument.
Warning: Indicates that failure to take note of the accompanying information
may result in personal injury.
Avertissement: Indique que ne pas tenir compte de l'information jointe peut entraîner
des blessures corporelles.
˜

UltiMate 3000 Series:
LPG-3400XRS Pump
Page 4 Operating Instructions
1.2.2 Safety Precautions
When working with analytical instrumentation, you must know the potential hazards of
using chemical solvents. Wear appropriate protective clothing.
Tip: Before initial operation of the pump, make yourself familiar with the
contents of this manual.
For the safety precautions in French, see page 8.
Warning: All users of the device must observe the following safety precautions
and all additional safety precautions in this manual to avoid the
possibility of personal injury or damage to the device when
operating the device or carrying out any maintenance or service
procedures.
Observe any warning labels on the pump and see the related sections
in these Operating Instructions.
•Protective equipment
When performing any work on or near the HPLC system, wear personal protective
equipment (protective clothing, safety gloves, safety glasses) as required by the hazard of
the mobile phase and sample. For information about the proper handling of a particular
substance and for advice on specific hazards, refer to the material safety data sheet for the
substance you are using. Observe the guidelines of Good Laboratory Practice (GLP).
An eyewash facility and a sink should be close to the device. If any substance splashes on
the eyes or skin, wash the affected area and seek medical attention.
•Hazardous substances
Many organic solvents, mobile phases and samples are harmful to health. Be sure that
you know the toxic and infectious properties of all substances that you are using. You
may not know the toxic or infectious properties of many substances that you are using. If
you have any doubt about a substance, treat it as if it contains a potentially harmful
substance. For advice on the proper handling of a particular substance, refer to the Safety
Data Sheet (SDS) of the manufacturer. Observe the guidelines of Good Laboratory
Practice (GLP).
Dispose of waste substance in an environmentally safe manner that is consistent with all
local regulations. Do not allow flammable, toxic, and/or infectious substances to
accumulate. Follow a regulated, approved waste disposal program. Never dispose of
flammable, toxic, and/or infectious substances through the municipal sewage system.

UltiMate 3000 Series:
LPG-3400XRS Pump
Operating Instructions Page 5
•Hazardous gases
Install the HPLC system in a well-ventilated laboratory. If the mobile phase or sample
includes volatile or flammable solvents, do not allow them to enter the workspace. If the
mobile phase or sample includes volatile or flammable solvents, avoid open flames and
sparks.
•Electrostatic discharge
Discharge of electrostatic energy may lead to sparking and can constitute a fire hazard.
This effect is particularly pronounced in insulating capillaries und with non-conductive
solvents (for example, pure acetonitrile).
Take appropriate measures to prevent the generation of static electricity near the HPLC
system. For example, make sure that the air humidity level in the laboratory is
sufficiently high and provide proper ventilation, wear anti-static clothing or shoes,
prevent accumulation of air bubbles in waste lines, and use grounded waste containers.
Use only non-conductive capillaries to direct solvents into the waste container. With
electrically conductive capillaries make sure that they are properly grounded.
•Self-ignition of solvents
Do not use solvents for which the self-ignition temperature is below 150 °C. In case of
leakage, these solvents may self-ignite on a hot surface.
•Capillaries, capillary connections, open connections
♦Capillaries, especially non-metallic capillaries may burst, slip out of their fittings or
may not be screwed in. This may result in substances spraying out of the open
connections.
♦In an UltiMate 3000 system, some components are made of PEEK™. This polymer
has superb chemical resistance to most organic solvents. However, it tends to swell
when in contact with trichlormethane (CHCl3), dimethyl sulfoxide (DMSO), or
tetrahydrofuran (THF). In addition, it is attacked by concentrated acids, such as,
sulfuric acid and nitric acid or a mixture of hexane, ethyl acetate, and methanol. In
both cases, capillaries may start leaking or they can burst. Swelling or attack by
concentrated acids is not a problem with brief flushing procedures.
♦Do not use tubing that is stressed, bent, kinked, or damaged.
♦Capillary connections can be contaminated by harmful substances or harmful
substances can escape from open connections.
♦Always wear safety glasses when handling fused silica tubing, for example, during
installation or when cutting capillaries to the length.
•Disconnect the pump from all power sources before removing the panels. When the
panels are removed, dangerous electrical connections will be exposed. The enclosure
must be opened only by Thermo Fisher Scientific service personnel.
•Always replace blown fuses with original spare part fuses authorized by Thermo Fisher
Scientific.

UltiMate 3000 Series:
LPG-3400XRS Pump
Page 6 Operating Instructions
•Replace faulty communication cables.
•Replace faulty power cords. Never use a power cord other than the power cords provided
for the device.
•Use only the original spare parts and accessories authorized for the device by Thermo
Fisher Scientific.
•The pump is primed with 2-propanol. During initial operation of the pump, make sure
that the solvents used are miscible with 2-propanol. Otherwise, follow the appropriate
intermediate steps.
•When operating the HPLC system, always set a lower pressure limit for the pump. This
prevents damage resulting from leakage or from running the pump dry.
•To prevent damage to the pump when lifting or moving, always lift the unit by the
bottom sides or sides. Do not lift the pump by the bottom front or front panel door. This
may damage the door.
•The open front panel door is not designed to carry weight. Do not place any heavy
objects on the open front panel door; this may damage the door.
•After operation, rinse out buffers and solutions that form peroxides.
•Before switching from buffer to organic solution, rinse the analytical system thoroughly
with deionized or HPLC grade water.
•When switching to another solvent, ensure that the new solvent is miscible with the one
contained in the HPLC system. If the solvents are not miscible, the system can be
damaged, for example, by flocculation.
•If a leak occurs, turn off the pump immediately, stop the pump flow, and remedy the
situation.
•Use only standard solvents (HPLC grade) and buffers that are compatible with all parts
that may be exposed to solvents.
•Avoid looking directly into the pump light LED and do not use light focusing instruments
for viewing the light beam. The high luminosity of the lamp can be harmful to the eyes.
•To avoid that the pressure calibration of the pump is impaired, turn on the pump only
when the pump pressure is down. To ensure that the pressure is down, open the purge
valve before turning on the pump.
•Never run the pump dry. Damage to the pistons or the piston seals could result.
•Before you start operating the pump, check the seal wash reservoir level and refill as
needed. After turning on the pump, wait until the wash solution has passed the entire
pump head.
•Always use fresh rear seal wash solution.

UltiMate 3000 Series:
LPG-3400XRS Pump
Operating Instructions Page 7
•Thermo Fisher Scientific advises against recycling the solvents. This may impair the
performance of the seals.
•If the pump flow is interrupted for longer periods (> 1 hour), turn off the lamps in any
UV or RF detector connected to the pump. This will prevent evaporation in the flow cell.
•Always use the frits recommended by Thermo Fisher Scientific to prevent particulate
matters from entering the HPLC system. Using other frits may considerably affect the
system performance.
•Before interrupting operation for several days or more or when preparing the pump for
transport, observe the precautions for shutting down the pump (→page 88).
•Do not use the pump in ways other than those described in these Operating Instructions.
•Keep the operating instructions near the device to be available for quick reference.

UltiMate 3000 Series:
LPG-3400XRS Pump
Page 8 Operating Instructions
1.2.3 Consignes de Sécurité
Si vous utilisez d'instrumentation analytique, vous devez connaître les risques d'utilisation
de produit chimiques.
Veuillez noter: Avant de commencer à utiliser l'instrument, assurez-vous que vous
vous êtes familiarisés avec le contenu de ce manuel.
Avertissement: Toutes les personnes utilisant l’instrument doivent observer les
consignes de sécurité suivantes et dans les autres chapitres de ce
manuel pour éviter une mise en danger de sa personne ou de
dommage à l’instrument pendant l’utilisation et des opérations de
maintenance ou service de l’instrument.
Observez les étiquettes d'avertissement sur l'instrument et référez-
vous aux sections correspondantes dans ce mode d'emploi.
•Equipment de protection
Pour tous les travaux sur le système HPLC ou à proximité, portez l'équipement de
protection personnel (vêtements de protection, gant de sécurité, lunettes de protection)
qui correspond aux risque découlant de la phase mobile et/ou de l'échantillon. Pour les
informations sur la manipulation correcte des composés et des recommandations pour les
situations de risque spécifiques, veuillez consulter la fiche de données de sécurité des
substances que vous utilisez. Veuillez respecter des directives des Bonnes Pratiques de
Laboratoire (BPL).
Une installation permettant de se laver les yeux ainsi qu'un lavabo doivent se trouver à
proximité du système. Si une substance, quelle qu'elle soit, entre en contact avec vos
yeux ou votre peau, rincez abondamment la zone affectée à l’eau, puis.
•Substances dangereuses
De nombreux solvants organiques, phases mobiles et échantillons sont nuisibles à la
santé. Informez-vous de propriétés toxicologiques et infectieuses de toutes les substances
que vous utilisez. Les propriétés toxicologiques et infectieuses de nombreuses substances
peuvent être mal connues. Au moindre doute concernant une substance, traitez-la comme
s'il contenait une substance potentiellement dangereuse. Pour des instructions comment
utiliser correctement des composés particuliers, veuillez consulter à la fiche de données
des sécurités du fabricant respectif. Veuillez respecter des directives des Bonnes
Pratiques de Laboratoire (BPL).
Débarrassez-vous de tous les déchets de substances de manière écologique,
conformément à la règlementation en vigueur au niveau local. Empêchez impérativement
l'accumulation de solvants inflammables, toxiques et/ou infectieux. Suivez un
programme d'élimination des déchets règlementé et approuvé. Ne jetez jamais de
solvants inflammables, toxiques et/ou infectieux dans le système municipal d'évacuation
des eaux usées.

UltiMate 3000 Series:
LPG-3400XRS Pump
Operating Instructions Page 9
•Gaz dangereux
Installez le système HPLC dans un laboratoire bien ventilé. Si la phase mobile ou
l’échantillon contient des solvants volatils ou inflammables, vous devez assurer qu'ils ne
pénètrent dans l'espace de travail. Si la phase mobile ou l’échantillon contient des
solvants volatils ou inflammables, évitez les flammes nues et les sources d’étincelles à
proximité.
•Décharge électrostatique
Décharge électrostatique peut provoquer la formation d'étincelles et peut présenter un
risque d’incendie. Veuillez noter que des solvants fluides dans les capillaires peuvent se
charger automatiquement. Cet effet se peut produire particulièrement forte dans les
capillaires isolants et avec des solvants non-conducteurs (par exemple, l'acetonitrile pur).
Prenez des mesures appropriées pour éviter les charges électrostatiques à proximité du
système HPLC. Par exemple, s'assurez qu'il y a une humidité de l'air suffisante et une
ventilation adéquate dans la laboratoire, portez des vêtements ou équipement de
protection antistatique, évitez l'accumulation de bulles d'air dans les lignes de déchets et
utilisez des réservoirs à déchets mis à la terre.
Utilisez uniquement des capillaires non-conducteurs pour diriger solvants au réservoir de
déchets. Capillaires électriquement conducteur devrait être mis à la terre.
•Inflammation spontanée des solvants
N’utilisez aucun solvants avec une température d‘auto-inflammabilité inférieure à
150° C. Si une fuite se produit, ces solvants peuvent s’auto-enflammer au contact d’une
surface chaude.
•Capillaires, connecteur capillaires, connexions ouvertes
♦Des capillaires, en particulier les capillaires non-métalliques, pourraient fendre ou
glisser des connecteurs ou ne peuvent pas être vissés. Ceci peut en résulter aussi que
des substances pourraient jaillir des connexions ouvertes.
♦Dans un système UltiMate 3000, certaines composantes sont en PEEK. Bien que ce
polymère présente une excellente résistance chimique à la plupart des solvants
organiques, il a tendance à gonfler lorsqu'il est en contact prolongé avec du
chloroforme (CHCl3), du diméthyle sulfoxide (DMSO) ou du tetrahydrofuran (THF).
De plus, il est attaqué par des acides concentrés tels que l'acide sulfurique et l'acide
nitrique ou d'un composé du hexane, éthyle acétate et méthanol. Ceci peut causer des
capillaires de fuite ou risquer des capillaires d’éclater. Ces acides peuvent cependant
être utilisés dans le cadre de procédures de nettoyage, à condition que l’exposition soit
brève.
♦N'utilisez pas de capillaires écrasés, pliés, abimés ou endommagés.
♦Les connecteurs capillaires pour pourrait être contaminé par des substances
dangereuses ou des substances dangereuses pourrait sortir des connexions ouvertes.
♦Portez des lunettes de protection lorsque vous manipulez des capillaires en silice
fondue (pendant l'installation, découpe, etc.).

UltiMate 3000 Series:
LPG-3400XRS Pump
Page 10 Operating Instructions
•Quand les capots de protection de l’appareil sont démontés, vous êtes exposés à des
connexions électriques sous haute tension deviennent accessibles. Débranchez
l'instrument de toute source d'alimentation électrique avant de retirer les capots. Ne
démontez les capots de protection que si cela est explicitement demandé au cours de ces
instructions. Les capots de protection devraient être démontés uniquement par le
personnel de service de Thermo Fisher Scientific.
•Remplacez toujours les fusibles grillés par des fusibles de rechange autorisés par Thermo
Fisher Scientific.
•Remplacez les câbles de communication défectueux.
•Remplacez les cordons d'alimentation électrique défectueux. Utilisez uniquement les
cordons d’alimentation électrique spécifique à l’instrument.
•Utilisez seulement des pièces de rechange originales et des accessoires autorisés par
Thermo Fisher Scientific.
•La pompe est stockée sous 2-Propanol. Au cours démarrage de la pompe, assurez-vous
que les solvants utilisés soient miscibles avec le 2-Propanol. Sinon, suivez les étapes
intermédiaires appropriées.
•Réglez toujours une limite de pression minimum pour la pompe HPLC. Ceci prévient les
dommages résultant de fuites ou de long-terme fonctionnement à sec de la pompe.
•Lorsque vous soulevez ou l’instrument, tenez-le toujours par le dessous ou par les côtés
de l'unité. Soulever l’instrument par la partie avant inférieure ou par le panneau avant
peut endommager la porte.
•Ne placez aucun objet lourd sur la porte ouverte du panneau avant. Ceci pourrait
endommager la porte.
•Après utilisation, purgez le système des tampons et des susceptibles de former des
peroxydes.
•Lorsque vous passez d’une solution saline à un solvant organique, effectuez un rinçage
intermédiaire du système HPLC à l'eau dé-ionisée ou qualité HPLC.
•Lorsque vous passez à un autre solvant, assurez-vous que le nouveau solvant soit
miscible avec celui qui se trouve dans la pompe. Dans le cas contraire, la pompe peut être
endommagée; par exemple, par des floculations!
•Si une fuite se produit, arrêtez immédiatement l’instrument, stoppez le débit de la pompe
et remédiez au problème.
•Utilisez uniquement des solvants (qualité HPLC) et des solutions salines compatibles
avec les matériaux exposés phase mobiles.
•De nombreux solvants organiques et solutions salines sont toxiques. Informez-vous des
propriétés toxicologiques de toutes les phases mobiles que vous utilisez.

UltiMate 3000 Series:
LPG-3400XRS Pump
Operating Instructions Page 11
•Ne regardez jamais directement la DEL pour l'éclairage intérieur dans la pompe et ne
regardez pas du faisceau lumineux par des instruments qui focalisent le rayon lumineux.
L'intensité lumineuse de la lampe peut être nocive pour les yeux.
•Afin d'éviter que le calibrage de pression de la pompe ne soit pas entravé, mettez en
marche la pompe seulement quand la pompe est sans pression. Toujours ouvrez la vis de
purge avant mettre la pompe en marche.
•Ne faites jamais fonctionner la pompe à sec. Il peut en résulter des dommages aux pistons
ou aux joints de piston.
•Avant de mettre en marche la pompe, assurez-vous que le réservoir de rinçage du joint
arrière est rempli. Attendez jusqu'à ce que le rinçage du joint arrière ait été pompé par
toutes les têtes de pompe.
•Utilisez toujours le liquide frais pour le rinçage du joint arrière.
•Thermo Fisher Scientific déconseille le recyclage de solvants. Ceci peut affecter les
performances des joints de piston.
•Si le débit de la pompe est interrompu pour des périodes prolongées (> 1 heure), éteignez
les lampes de tout détecteur UV ou RF raccordé à la pompe. Ceci empêchera
l'évaporation dans la cellule.
•Utilisez toujours les frittés recommandés par Thermo Fisher Scientific afin d'empêcher
les particules étrangères d'entrer dans le système HPLC. Utiliser d'autres frittés peut
affecter considérablement les performances du système.
•Avant d'interrompre le fonctionnement pendant plusieurs jours ou plus, observez les
précautions figurant en page 88.
•N'utilisez pas l'instrument de manière autre que celles décrites dans ce manuel.
•Conservez ce manuel á proximité de l’instrument pour pouvoir le consulter facilement.

UltiMate 3000 Series:
LPG-3400XRS Pump
Page 12 Operating Instructions
1.3 Intended Use
For Research Use Only. Not for use in diagnostic procedures. The device is designed to be
operated only be qualified and authorized personnel. All users must know the hazards
presented by the device and the used substances.
The pump is designed for laboratory research use in high-performance liquid
chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC)
applications. It is part of the UltiMate 3000 system, but can also be used with other HPLC
systems if adequate control inputs and outputs are available. A PC with USB port is
required.
The pump is operated with the Chromeleon Chromatography Management System. Being
part of the UltiMate 3000 system, the pump can also be operated with other data systems,
such as
•Xcalibur™, Compass™/HyStar™, Atlas, or Analyst®
To do so, installation of the DCMSLink (Dionex Chromatography Mass Spectrometry
Link) software is required in addition to the installation of the data system.
•Empower™
Installation of the Dionex Instrument Integration Software is required in addition to the
installation of the data system.
For more information, contact the Thermo Fisher Scientific sales organization for Dionex
HPLC Products.
Note that the pump may be operated only with accessories and spare parts recommended
by Thermo Fisher Scientific (→page 137) and within its technical specifications
(→page 135).
If there is any question regarding appropriate usage, contact Thermo Fisher Scientific before
proceeding.
Warning: If the device is used in a manner not specified by Thermo Fisher
Scientific, the protection provided by the device could be impaired.
Thermo Fisher Scientific assumes no responsibility and will not be
liable for operator injury and/or instrument damage. Whenever it is
likely that the protection is impaired, the instrument must be
disconnected from all power sources and be secured against any
intended operation.
Avertissement: Si l'instrument est utilisé de façon non spécifiée par Thermo Fisher
Scientific, la protection prévue par l'instrument pourrait être altérée.
Thermo Fisher Scientific n'assume aucune responsabilité et ne sera
pas responsable des blessures de l'operateur et/ou des dommages de
l'instrument. Si la protection de l'instrument n'est pas garanti à tout
moment, débranchez l'instrument de toutes les sources d'alimentation
électrique et assurez-vous que l'instrument n'est pas utilisé
involontairement.