UVP biodoc-it 2 Guide

81-0358-01 Rev E
BioDoc-It®2Imaging System
Installation and User Instructions
UVP, LLC Ultra-Violet Products Ltd.
2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]
Web Site: www.uvp.com

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Table of Contents
Table of Contents................................................................................................................................................... 2
Introduction........................................................................................................................................................... 3
System Components .............................................................................................................................................. 4
Specifications ............................................................................................................................................................4
Built-In Touch Screen Computer................................................................................................................................5
Cameras and Lenses..................................................................................................................................................5
Ethidium Bromide (EtBr) Emission Filter ...................................................................................................................5
Darkroom ..................................................................................................................................................................5
Transilluminator........................................................................................................................................................6
LCD Touch Screen ......................................................................................................................................................6
VisionWorks touch Software.....................................................................................................................................6
Fluorescent Focus Target ..........................................................................................................................................6
USB Flash Drive .........................................................................................................................................................6
Optional Equipment ..................................................................................................................................................6
Setup Instructions.................................................................................................................................................. 7
Components ..............................................................................................................................................................7
Connecting the Power Cables ...................................................................................................................................7
Installing Emission Filters..........................................................................................................................................7
Camera Setup and Installation..................................................................................................................................7
The camera and zoom lens are assembled at the UVP factory.................................................................................7
Note: The zoom lens shipped may appear different than pictured...........................................................................7
Using the System ................................................................................................................................................... 8
Powering Up the Tablet ............................................................................................................................................9
Operating the VisionWorks touch Interface..............................................................................................................9
Using the Transilluminator .......................................................................................................................................9
Using the Epi (Overhead) White Light.....................................................................................................................10
Using the UV Gel Viewer Window...........................................................................................................................10
Image Focusing .......................................................................................................................................................10
Image Zooming .......................................................................................................................................................10
Touch Screen Interface ........................................................................................................................................ 11
Setting User Preferences .........................................................................................................................................11
Identifying the Touch Screen Buttons and Functions ..............................................................................................13
Using Templates .....................................................................................................................................................18
Connecting to a Network ........................................................................................................................................19
Installing Drivers or Additional Software ................................................................................................................19
Service Procedures .............................................................................................................................................. 20
Return Procedure ....................................................................................................................................................20
Replacement Parts and Accessories........................................................................................................................20
Troubleshooting ......................................................................................................................................................21
Replacing Transilluminator Bulbs ...........................................................................................................................21
Follow these steps to replace the UV tubes in the system’s transilluminator:........................................................21
Technical Support....................................................................................................................................................22

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Introduction
The BioDoc-It2Imaging System enables simple documentation of fluorescent and non-fluorescent gels,
film plates and assays with the ability to save images to a USB flash drive or network location for later
quantitative analysis or enhancement for publication.
Images are saved in a variety of selectable formats, allowing saved images to be read by most PC or Mac
programs. The BioDoc-It2Imaging System is a cost effective solution for capturing quality images in a
compact standalone package, as no external computer is required.
The BioDoc-It2comes standard with the GelCam 315 camera, which includes a 5.0 MP (2592 x 1944)
resolution, an 8-48mm f/1.2 zoom lens, and 16-bit file bit depth. Multiple transillumination options are
available, including single UV (302nm), 2UV™ (302/365nm) and 3UV™ (302/365/254nm) versions with
illumination areas ranging from 20 x 20cm to 25 x 26cm
*System/software configurations may vary by country. Contact UVP or authorized distributor for details.

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System Components
Refer to the packing slip and pictured components below for specific parts and components included with
the system.
10” Integrated Touch
Screen
White Light and UV
Power Buttons
Wide Access
Door
USB Port (Not Shown)
Two-Position Filter Tray,
Including EtBr Filter
Tablet power switch
UV Gel Viewer
Window
GelCam 315 Camera and
Manual Zoom Lens
Transilluminator
Controls
Side Access Door

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Specifications
Power Requirements: 100/115V, 50/60Hz; 3.1 Amps at 120 Volts
230V, 50/60Hz; 1.55 Amps at 230 Volts
Mains supply voltage fluctuations are not to exceed 10 percent of the nominal
supply voltage
Pollution Degree: 2
Installation Category: II
Altitude: Up to 2000m
Ambient Temperature: 5°C to 40°C
Humidity: Maximum relative humidity of 80% for temperatures up to 31°C, decreasing
linearly to 50% maximum relative humidity at 40°C
Built-In Touch Screen Computer
Size
10.1“ touch screen
Screen
Multi-touch, 1280 x 800 Resolution
Operating System
Windows® 10 Home (32 bit)
Hard Drive Capacity
64 GB Flash Storage
Memory
2 GB RAM
Wireless Networking Capability
802.11 b/g/n Wi-Fi and Bluetooth 4.0
Connectivity Ports
1 USB (side of system)
6 USB (rear of system)
Software
VisionWorks touch Software
Cameras and Lenses
The BioDoc-It2315 is equipped with the GelCam 315 Camera, with a resolution of 5.0MP. The system is
equipped with an 8-48mm zoom lens, allowing the user to zoom in and out on the sample within the
darkroom.
The GelCam 315 uses a USB 2.0 connection. All camera settings are factory pre-set for optimum
performance when viewing gels and films under low light level conditions. Contact UVP Technical Support
before making any adjustments to camera settings.
Ethidium Bromide (EtBr) Emission Filter
The ethidium bromide (50mm2) UV-blocking bandpass interference filter blocks UV and IR radiation emitted
from the transilluminator. The filter is placed in the two-position emission filter assembly and allows
visualization of fluorophores from 580-630nm, targeting the ethidium bromide emission peak of 605nm.
Additional filters are available for other specific fluorophores, including custom filters. Filters can also be
removed when imaging non-fluorescent media (including protein gels, colony plates, etc.) in order to
produce brighter images. Contact UVP for ordering information.
Darkroom
Darkroom features include:
Integrated 10” touchscreen interface
Integrated transilluminator and epi (overhead) LED white lights
UV-safe gel viewer window built into the darkroom door
Side doors for easy access to the interior of the darkroom (for placement or excision of samples)
Two-position emission filter selector
UV safety interlock switch to disable UV transillumination when the main darkroom door is opened

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Fluorescent Focus Target
UV/White Converter Plate
Transilluminator
The BioDoc-It2Imaging System includes an integrated UVP Benchtop transilluminator. UVP offers a variety
of transilluminator configurations, including models with multiple wavelengths and variable intensities.
LCD Touch Screen
The BioDoc-It2Imaging System contains a fully integrated 10.1-inch color touchscreen computer. The touch
screen allows the user to perform a variety of tasks, including previewing, capturing, saving and printing
images, as well as selecting preference options, without the use of an external mouse or keyboard.
For users who prefer not to use the touch screen interface, an external keyboard and mouse can be used
via any available system USB ports.
VisionWorks touch Software
Image acquisition functions for the BioDoc-It2are controlled using the VisionWorks touch software interface.
Software features include image preview, capture and save functions.
For more information, see the Touch Screen Interface section in this manual.
Fluorescent Focus Target
The UVP Fluorescent Focus Target fluoresces when placed on a
UV transilluminator or when exposed to overhead UV. The Target
provides sharp fluorescent images to aid in adjusting the lens and
camera settings for ideal imaging results.
USB Flash Drive
The removable USB flash drive, included with the system, has 8GB memory (minimum) and connects to the
system to allow for saving and transferring of images.
Optional Equipment
UVP offers a variety of optional equipment to support the needs of varying laboratory environments. Refer to
Replacement Parts and Accessories at the end of this manual for optional equipment part numbers.
Thermal Printer
The thermal printer provides archive quality, 256 grayscale prints and five optional cost-effective print
sizes.
Converter Plates
The UV/White Converter Plate allows imaging of non-
fluorescent stained media with an ultraviolet transilluminator.
The converter plate is specially coated to convert 302nm UV
to white light.
The Visi-Blue™Converter Plate (not shown) converts UV
to a safe 460-470nm wavelength designed for use with blue
excitation samples and SYBR Green, SYPRO Orange and
GFP stains.

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Connection for the
main darkroom
power
Connection for
jumper cord to the
transilluminator
Setup Instructions
Components
When unpacking the BioDoc-It2Imaging System, the following items will be included:
1. BioDoc-It2system with integrated transilluminator
2. Camera and zoom lens
3. Power, jumper and USB cables
4. Ethidium bromide (EtBr) emission filter
5. USB flash drive (8GB minimum)
6. Supporting documentation
7. USB flash drive containing VisionWorks touch Software and
8. USB flash drive containing VisionWorks Acquisition and Analysis Software (only for use on an external
computer)
Place the darkroom on a flat surface which can provide adequate support for up to 50 pounds.
WARNING: Do not attempt to perform any setup procedures while the system is plugged in or powered on
unless otherwise instructed.
CAUTION: Do not install the system in areas with high moisture, dust or high temperatures. Keep the
equipment away from motors or any other large magnetic equipment. This system is designed for indoor use
only.
Connecting the Power Cables
1. Plug the main power cable into the back of the
darkroom and the other end into a surge-protected
power outlet.
2. Connect the jumper cable from the darkroom to the rear
of the transilluminator.
Installing Emission Filters
To install the 50mm2ethidium bromide (EtBr) filter and any other emission filters:
1. Carefully remove the filter from the protective plastic case, holding the filter at the edges to prevent
placing fingerprints on the glass surface.
2. The 2-position filter tray is located at the top of the system, below the camera lens hole. Place the
emission filter in the desired position by inserting the filter through the camera lens hole and into the
desired filter tray position. Note: The camera and lens must be removed to access the filter tray.
Additional and replacement emission filters are available through UVP. Refer to the Replacement
Parts and Accessories section of this manual for ordering information.
Camera Setup and Installation
The camera and zoom lens are assembled at the UVP factory.
Note: The zoom lens shipped may appear different than pictured.
1. Remove the cap from the lens.
2. If not already assembled, attach the step-
up ring and diopter to the lens. The step-
up ring and diopter will only fit one way.
3. Using the two brass thumb nuts provided,
secure the camera assembly bracket to
the darkroom bracket located on the top
of the BioDoc-It2. Ensure that a light-tight
Step-up ring
Diopter
Camera assembly bracket

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seal is made between the end of the lens and the rubber gasket beneath the darkroom bracket.
4. Plug the camera cable into the top of the camera and the other end into a USB port on back of the
darkroom.
Camera and lens
assembly
Camera USB cable
Darkroom bracket
Brass thumb nut
Camera bracket
Rubber gasket

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Using the System
Powering Up the Tablet Computer
1. Firmly press the POWER button on the system, and hold for 3 seconds to power on the internal
computer. Wait for the Windows startup screen. This may take a few moments.
2. Once the computer completely boots, the software will load automatically. If it does not, double click on
the VisionWorks icon.
3. To SHUT DOWN the computer, briefly press the POWER button.
Note: The tablet will remain on battery power if the main power cable to the whole system is unplugged.
Operating the VisionWorks touch Interface
Upon startup, the internal computer will proceed through the boot-up process. When complete, the Windows
desktop will appear. The software interface, similar to the one below, will open automatically shortly
thereafter.
To exit the software interface, press either the close (X) or minimize (_) buttons at the top right corner of the
software window.
Refer to Touch Screen Interface in this manual for further instructions on using the software.
Using the Transilluminator
Once the BioDoc-It2darkroom is plugged in to a power outlet, power is supplied to all components. This
includes the jumper cable that supplies power to the transilluminator.
To use the transilluminator:
1. Open the darkroom door and place the main power switch located on the front of the transilluminator in
the ON (I) position. (Note: It is recommended to always leave this power switch in the ON (I) position.)
2. Use the rotary knob on the front of the transilluminator to select from the available lighting wavelengths
or intensities (transilluminator settings vary by model).
3. Locate the UV power button on the front of the BioDoc-It2system, to the right of the touch screen. Press
this switch until it glows green. The transillumination is now active.
4. Press the switch again to turn off the transilluminator.

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Refer to the transilluminator manual for additional instructions on using the transilluminator.
Note: The BioDoc-It2integrates a UV interlock switch which will inactivate the UV transilluminator when the
main darkroom door is open. This switch is located on the upper right corner of the darkroom door opening
and is only accessible when the main door is open.
Using the Epi (Overhead) White Light
1. Locate the White power button on the front of the BioDoc-It2system, to the right of the touch screen.
Press this switch until it glows green. The epi (overhead) white light is now active.
2. Press the switch again to turn off the epi (overhead) white light.
Using the UV Gel Viewer Window
The UV Gel Viewer Window, built into the main darkroom door, allows users to view the interior of the
darkroom without opening the main door. The window glass is UV blocking while providing a clear view to
the transilluminator surface for sample viewing.
To open the window cover, pull down on the metal tab. The cover will pop open. To close the cover, pivot it
up until it reengages with the two small magnets.
NOTE: Close the UV Gel Viewer Window prior to capturing light-sensitive images.
Image Focusing
Prior to capturing an image, prepare the image focus as follows:
1. Remove the blue protective film from the Fluorescent Focus Target (see the “Fluorescent Focus
Target”section of this manual for more information).
2. Turn on the transilluminator and place the Target on the transilluminator surface.
3. Using the VisionWorks touch, press Start Preview to begin viewing the sample within the system.
Adjust the focus, zoom and aperture controls using the Focus,Zoom and Aperture rings on the
camera lens until an ideal image is visible.
Refer to the Touch Screen Interface section in this manual for further instructions on using the software.
Image Zooming
The BioDoc-It2is equipped with an optical zoom lens, meaning that the system uses the lens’optics to make
the sample appear closer/larger on the screen. Optical zoom is adjusted using the Zoom ring on the camera
lens.
However, it may be desirable to use digital zooming to move in closer on the image. Digital zoom enlarges a
portion of the image, simulating optical zoom. Thus, the camera crops a portion of the image and enlarges
the cropped portion to fill the imaging area on the screen.
To use digital zoom functionality:
1. With a preview or captured image on the screen, use the “+”and “–” buttons located on the right side
of the image.
2. Tap and drag to move around on the zoomed-in image.
Notes regarding digital zoom operation:
The software’s digital zoom feature utilizes WYSIWYG, or “what you see is what you get,” meaning that
a zoomed preview image will result in a zoomed capture image.
A zoomed capture image will save and print as shown on the screen (WYSIWYG).