V&P Scientific SPINVESSEL VP 418SV2-H-1LFB-CC User manual

V&P Scientific, Inc. 1 TECHNICAL NOTE 457
TECHNICAL NOTE 457
OPERATION MANUAL FOR
CIRCLE HEATER SPINVESSEL®
VP 418SV2-H-1LFB-CC
*US and Foreign Patents Pending
Figure 1. Basic Parts of Circle Heater SpinVessel® VP 418SV2-H-1LFB-CC
(SpinVessel® Controller not shown).
WARNING!!!!!
The VP 418SV2-H-1LFB-CC Circle Heater SpinVessel®will be very hot when operating, so
exercise caution. Do not handle red aluminum heating portion of the unit while it is operating,
it will be HOT!!
Temperature
Controller
Circle
Heater
SpinVessel
Motor Unit

V&P Scientific, Inc. 2 TECHNICAL NOTE 457
Figure 2. Parts of the Circle Heater SpinVessel® VP 418SV2-H-1LFB-CC.
Figure 3. Back of Temperature Controller (left). Cable and plugs (right)
for connecting Circle Heater to Temperature Controller.
Load
Power
Sensor
Load
Sensor
On/Off
Switch
Temperature
Controller
SpinVessel
Controller
Temperature
Sensor
Temperature
Controller
Power Cord
Temperature
Controller
Cable
USB
Drive
USB
Cable
Temperature
Sensor
Hole Plugs
SpinVessel
Controller
Power
Supply and
Cable
SpinVessel
Controller
Power Cord
SpinVessel
Controller
Cable
Circle Heater
SpinVessel
SpinVessel
SpinBase

V&P Scientific, Inc. 3 TECHNICAL NOTE 457
Figure 4. Connecting parts of the Circle Heater SpinVessel®.
SET-UP
CIRCLE HEATER SPINVESSEL INSTALLATION
1. Carefully remove Circle Heater Spin Vessel, Temperature Controller and SpinVessel Controller from the
packaging inside the shipping box. Place the Circle Heater Spin Vessel on a solid work surface that is clear of
heat-sensitive items, such as paper or equipment, or heat-conducting material. Both the Temperature and
SpinVessel Controllers can be placed next to the Circle Heater Spin Vessel or up to 6 feet away with respective
Controller Cables. Test on the benchtop before using with a liquid handler to become familiar with its
operation.
2. The Temperature Controller Cable, which is attached to the back of the Circle Heater, has two plugs on the
free end for connecting to the Temperature Controller (Figure 3, page 2). Connect the round 3-pin plug of
the Connector Cable into the receptacle marked “Load” on the back of the Temperature Controller as shown
in Figure 3. Also, on the back of the Temperature Controller, connect the 2-prong plug of the Controller Cable
into the receptacle marked “Sensor”.
3. See Technote 378B for instructions on setting up and running the SpinVessel part of the Circle Heater Spin
Vessel. A laptop or PC is needed to control the SpinVessel.
4. Connect the power cord to the power receptacle on the back of the Temperature Controller. Connect the
power cord to a 120 V outlet.
Temperature
Controller
SpinVessel
Controller
Temperature
Sensor
Controller
Temperature
Controller
Power Cord
Temperature
Controller
Cable
< 6 feet >
USB
Cable
Temperature
Sensor
SpinVessel
Controller
Power Supply
and Cable
SpinVessel
Controller
Power Cord
SpinVessel
Controller
Cable
< 6 feet >
Circle Heater
SpinVessel
SpinVessel
Motor Unit

V&P Scientific, Inc. 4 TECHNICAL NOTE 457
5. The Circle Heater has two temperature sensors, one internal and one external. The internal one controls the
heating of the Circle Heater. The external one, composed of a control box and a non-contact infrared sensor,
can monitor the temperature of the heated and mixed solution (see Figure 5). The Temperature Sensor Controller
can be easily attached to the Circle Heater by engaging the magnets that are inside the Controller and with the
ones in the side of the Heater. Just be careful to keep fingers away from the back of the Controller when doing
this as the magnets are strong and fingers can be pinched!
6. There are 2 holes for Temperature Sensor placement, use the one that works best for the pipetting head to be
used. The other one can be covered with the provided plug.
Figure 5. Attach the external Temperature Sensor Controller.
Figure 6. Position the external Temperature Sensor and take a reading by pushing the blue button.
Temperature
Sensor
Opening for
Pipet Tip
Access
Temperature
Sensor
Push Button
Temperature
Sensor
Display
Hole for
Temperature
Sensor
Temperature
Sensor
Temperature
Sensor
Controller
Magnets
Holes for
Temperature
Sensor
Temperature
Sensor
Hole Plug

V&P Scientific, Inc. 5 TECHNICAL NOTE 457
OPERATION
HEATING BLOCK OPERATION USING THE TEMPERATURE CONTROLLER
1. Turn on the Temperature Controller using the on/off power switch (see figure 7). The Controller already has a
temperature programmed so the Circle Heater will immediately begin to heat. The red number in the upper
display is the current temperature of the heater. The green number in the lower display is the value set during
operation. See the Tempo Controller Operating Instructions (Appendix) for more details on the features of
the display.
2. To turn off the Circle Heater without turning the power switch off, press the down arrow key until the green
lower display reads “OFF”.
Figure 7. Front View of Temperature Controller for the Circle Heater.
3. The upper limit temperature on the Temperature Control Panel is set to 200oC at the factory. The temperature
of the Spin Bases should not be higher than 120oC because of the Polypropylene SpinVessels. Polypropylene
will melt at temperatures greater than 130oC.
4. Temperature set value will need to be empirically determined. Since there is no direct contact between the
polypropylene SpinVessel and the heating element, heat transfer must occur through the air and to a limited
extent through the Spin Base of the SpinVessel motor unit. Therefore, the temperature setting for the Controller
will be much higher than the actual temperature in the solution in the SpinVessel.
5. It is best to pre-heat the Circle Heater and the solution to be mixed as it takes a long time for the solution to
come to temperature otherwise. Heat the solution in a beaker so that it can be poured into the 1 liter SpinVessel
already placed in the Circle Heater’s Spin Base (see figure 8 next page).
6. If the lid was on during the pre-heating of the Circle Heater, be careful when handling it as it will be very hot!
Place it back on top after pouring the heated solution into the 1 liter SpinVessel.
7. Note that the position of the Circle Heater in the photos has the SLAS dimensioned SpinVessel Motor Unit in
a “landscape” position as it would be held in most microplate locators on a liquid handler deck. The magnets
that hold the Temperature Sensor Controller will be in the front and if the lid is placed so that the “VP” label is
toward the back, the opening in the lid will mirror the position of the Motor Unit underneath.
On/Off
Power Switch
Upper Display:
Process Value
Lower Display:
Set Value
Scroll
Key
Up/Down
Arrows
Return
Key
Temperature Control Panel

V&P Scientific, Inc. 6 TECHNICAL NOTE 457
8. Since the hole for the pipet tips is so large, the solution will gradually lose heat over time. Testing has shown
an approximately 4 degree reduction over 3 hours with 60oC water. When the Controller was set at 190oC the
sides of Circle Heater got to 78oC, so be careful when handling the Circle Heater.
Figure 8. Pouring pre-heated solution in SpinVessel in the Circle Heater. Replacing the lid in the
proper orientation for use with 96 channel pipet head. Independent check of solution temperature.
OPERATION of the SpinVessel
Please refer to the technical information provided in Technote 378B. Please contact V&P Scientific for more
information.
TEMPERATURE CONTROLLER OPERATING INSTRUCTIONS
Supplied as an attached document.

V&P Scientific, Inc. 7 TECHNICAL NOTE 457
Product Maintenance
GENERAL PRODUCT CARE
When not in use, turn the power switch off.
Do not place the Temperature Controller in chambers with temperatures above 40oC.
Do not submerse Circle Heater or Temperature Controller into liquid or allow liquid to come into contact with
any openings.
Do not for any reason open the Temperature Controller enclosure or the heating element compartment of the
Heating Block. This will void the warranty.
For any other questions regarding the Controller, please refer to the Temperature Controller Instructions
(Appendix).
If technical assistance is required, contact:
V&P Scientific, Inc.
Ph: 858-455-0643
[email protected]
WARRANTY
V&P Scientific, Inc. warrants this product to be free from defects in material and workmanship when used under
normal laboratory conditions for one year. This warranty begins on the date of delivery from V&P Scientific.
In the event this product fails under normal laboratory conditions within the specified period of time because of
a defect in material or workmanship, V&P Scientific will, at its option, repair or replace the product. Damage to
the product caused by user negligence is not covered.
Please keep the special shipping carton in case the unit needs to be shipped back to V&P Scientific. Please
contact V&P Scientific at the above address for return authorization and shipping instructions.
This warranty is made in lieu of other warranties expressed or implied including the warranties of merchantability
and fitness for a particular purpose. V&P Scientific shall not be liable for loss or damages arising from the use
of these products nor for consequential damages of any kind.