Walter Products OL-D-100 User manual

INSTRUCTION MANUAL
for
Olivia Series
(A) CONTENTS
A. Contents…….……………………………………………………………………...…………………..……..………….Cover
B. Illustration of Microscope…….…………………………………………………………….………………..………..…....1
C. Unpacking and Preparation for Use…..…………………………………………………………………….…….………2
D. Features……………………………………………………………………………………………………….……………..2
E. Operating Procedure……….……………………………………………………………………….….………..……..…2,3
F. Maintenance and Care…………………………………………………………………………………….…………..……3
G. ElectricalMaintenance..………….………………………………………………………………..………………………4
H. Troubleshooting………………..…….……………………………………………………………..……..…………………..4
I. Warranty……….……………………………………………………………………………………………………………..4
“PLEASE READ INSTRUCTIONS BEFORE USING MICROSCOPE”
Model:
OL-D-100
10x Eyepiece
Separate
Course & Fine
Focus Knob
Mechanical Stage
Head
Revolving
Nosepiece
Illuminator Switch
Base
Stage Clips
DIN Objectives
Iris Diaphragm
LED Illuminator
Source
Ocular Tube
Nosepiece
Objective Turret
Arm-Ergonomic design
for convenient lifting
Abbe Condenser
Head Locking Screw
X-Y Control Torque

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OLIVIA SERIES MICROSCOPES
(C) UNPACKING & PREPARATION
The Olivia Series microscopes are supplied with an expanded two-part Styrofoam/polystyrene case. This case should be used for
storage, transport, and shipping. Microscopes that are not properly packed are usually damaged during transport andshipping.Ifyour
microscope arrives damaged during transport, contact the transportdept.tofilea claim. KEEPSTYROFOAMBOXFORFUTUREUSE
FOR SHIPPING AND/OR REPAIR.
Unpack the microscope and its parts carefully. Do not throw away any boxes or packing materials until the contents of the shipping
container have been checked against the packing list of your order. In a clean, dry, dust free environment, place the microscope and
parts on a sturdy desk or table for initial use and/or assembly. DO NOT TOUCH ANY LENS SURFACES WHILE HANDLING THE
MICROSCOPE. THIS MAY ADVERSLEY AFFECT IMAGE QUALITY.
(D) FEATURES AND DEFINITIONS
EYEPIECE: Locked in, 10X wide field eyepiece with built in calibrated pointer. Optional 16X wide field eyepiece is available.
DIOPTER ADJUSTMENT: The diopter is used to focus the eyepiece in conjunction with the fixed eyepiece to give equal
sharpness to both viewers.
HEAD: 360º rotatable. Choices of Monocular (for one viewer), Double 45º Dual View (for two viewers), 90º Dual Video (one
viewer and camera mount), or Binocular (for viewing with both eyes).
OBJECTIVES: Lower functional component of the optical system. Available with DIN standard 4x, 10x, 40x and 100xR oil. With
the 10x Eyepiece the superior-quality objectives provide magnification of 40x, 100x, 400x and 1000x.
FOCUSING KNOBS: The microscope is equipped with separate coarse and fine focusing knobs or your choice of coaxial focusing
referring to the separate and course knob mounted on the same axial. The course knob is the larger knob on the
outside and the fine knob is smaller and located on the inside.
CONDENSER: The microscope is equipped with either a 0.65 N.A. Condenser or 1.25 N.A. Abbe Condenser. The condenser fills
the back lens element of the objective to improve the resolution of an image.
DIAPHRAGM: The microscope is equipped with either a Disc or Iris Diaphragm. The disc diaphragm is located beneath the stage
and rotates exposing holes with various apertures to help achieve better resolution. Use large apertures for lower
magnifications and smaller apertures for higher magnifications.
ILLUMINATOR: LED light source, which illuminates the specimen. LED is a cooler, white light source with a low voltage providing
hours of use for your microscope.
STAGE: Includes a X-Y built-in mechanical stage with 0.1mm increments for precise measurement. To ensure long life for the
mechanical gears, always turn the focusing knobs slowly and uniformly. Use the Control Torque knobs to move the specimen
slide in the x-y direction.
SAFETY RACK STOP: Controls the maximum upward movement of the stage. When properly adjusted prevents high-powered
objectives from breaking a specimen slide.
RECHARGEABLE MODELS: Choose from Rechargeable or Non-Rechargeable models. RC= Rechargeable Nickel Metal Hydride
(NiMH) batteries included for up to 10,000 hours of bulb life.
SPECIFICATIONS (With 10X Eyepiece):
Objective
Total Magnification
Field of View
N.A.
4X
40X
4.5mm
0.10
10X
100X
1.8mm
0.25
40X
400X
0.45mm
0.65
100X Oil
1000X
0.18mm
1.25
(E) OPERATING PROCEDURE
I. How to use your Microscope
(For models equipped with a standard disc diaphragm)

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1. Select a position to work where little direct light falls on the instrument. Avoiding placing the microscope near or facing a
large window as direct light may fall directly on the microscope and adversely affect the contrast and resolution.
2. Turn the disc diaphragm so that the number 2 or 3 opening is aligned with the in-stage condenser.
3. Place a low powered objective into position and adjust the plain side of the mirror until the field is evenly illuminated. No
adjustments are necessary if the microscope is equipped with an illuminator; simply plugitinto anygroundedreceptacle.
4. Place the micro-slide specimen to be observed under the spring stage clips. If using a mechanical stage, pull back the
lever on the left side of the stage, insert the slide, then bring the crescent shaped holder into contact with the slide. Be
certain that the cover slip of the slide is facing towards the objective; otherwise you will not be able to focus your
specimen at high magnifications.
5. Position the specimen so that it is centered over the in-stage condenser.
6. Focus the objective on your specimen by turning the LARGE COARSE ADJUSTMENT KNOBS until the image of your
specimen is bright and clear. Microscopes will always lower the objective to a point that they know is beneath the focal
plane and focus upwards. Now you can bring the specimen into sharp focus by turning the SMALLER FINE FOCUS
KNOBS.
7. With the specimen now in sharp focus rotate the nosepiece to the other objectives and focus using only the fine focus
knobs. Since the optics on the Microscopes is Par-focal and Par-centered only a slight turn of the fine focus knobs with
be necessary.
NOTE: It is important to note that because of our built–in stop the 4X and 10X objectives can nevercome intocontact withyour
microscope slides. The 40XR and 100XR may occasionally touch the micro-slide but because these objectives have
retractable mounts your slide will not be damaged.
8. Adjust the disc diaphragm until proper specimen contrast is reduced.
9. You are now ready for microscope observation.
II. How to use the microscopes equipped with a 1.25 Abbe-condenser with or without the 100XRoil immersionobjective.
Same as steps I.1 through I.6 above
7. FOCUSING THE CONDENSER. The sub-stage Abbe condenser is mounted beneath the stage in a spiral-focusing
mount. To focus the condenser, loosen the large lock screw facing you as you look at the front of the
condenser. Twist the base of the condenser to the left to lower and to the right to raise. Adjust the condenser until the
illumination of the field is uniform.
8. Adjust the condenser iris diaphragm to match the N.A. of the objective. This is done by first closing the iris and opening it
slowly until the entire filed is evenly and brightly lit and in good focus. If the objective is changed to a higher power, the
iris must be adjusted to the new objective.
9. USE OF THE 100XR, OIL IMMERSION OBJECTIVE. Oil immersion objectives are used for the highest magnification.The
microscope tube is racked up and the 100XR objective swung into place. Place a drop of good quality immersion oil on
the slide and focus down with the coarse adjustment until the lens makes contact with the oil. Continue (more slowly) to
focus down with coarse adjustment until the color or a blurred outline of the specimen just appears. Now complete the
focusing with the fine adjustments.
In using the 100X objective, the most favorable resolution is obtained with the Abbe condenser nearly touching the slide
specimen. Ideally, a drop of immersion oil is placed between the condenser and the slide, as well as between the slide
and the 100X objective. Although this practice is not always followed in routine study, it is the only way to take full
advantage of the inherent resolution of the 1.25 N.A. Abbe condenser.
(G) MAINTENANCE & CARE OF YOUR MICROSCOPE
Cleaning of the optical surfaces - Never take objectives or eyepieces apart. They should be cleaned on the instrument since
they are not easily removed. To clean lens surfaces, first remove dust using a soft brush or blow off with a small syringe. Use a
cotton-tip applicator and a small amount of xylene. Clean only the front lens element of the objective and the top lens of the
eyepiece. Wipe again with a clean cotton-tip and, finally, blow or brush off the lens surfaces. The mirror or illuminator lens
surfaces may be cleaned in the same manner, but better results may be obtained by wiping with a soft lint free cloth.
Cleaning and Lubricating of Mechanical parts - This type of maintenance should be done by an authorized technician and will
help insure many years of trouble free use of your microscope.
Adjustment for body drift - Tension control adjustment collar is located between arm and coarse focus adjustment knob.Witha
small jewelers type screwdriver, loosen locking setscrew located in one of four holes on the collar. Rotate collar clockwise until
stage drift is eliminated. Re-tighten locking set screw to prevent accidental movement of collar.

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(H) ELECTRICAL MAINTANENCE (BULB REPLACEMENT)
The LED Rechargeable models ensure your microscope is fully charged for your first time use. It is necessary to
plug your microscope adapter to a suitable power outlet and allow charging for approx. 8-10 hours to be fully
charged. Gives up to 50 hours of continuous use per charge. Battery life –500 recharge cycles.
To replace the bulb, swing open the bottom door on the bottom of the base for easy access to change the light bulb.
LED- (Light Emitting Diode) bulbs generate cool illumination, contain a low voltage, and emit a white light for easy
viewing. (Replacement Bulb: Part No. LBB13)
SYMBOLS –This microscope may have various symbols attached. Please be aware of their meanings.
- “ATTENTION, CONSULT ACCOMPANYING DOCUMENTS” - “HIGH TEMPERATURE PARTS SYMBOL”
- “PROTECTIVE EARTH SYMBOL” - ”DOUBLE INSULATED SYMBOL”
(I) TROUBLE SHOOTING
Stage drops out of focus
If you notice that when you focus on a specimen the stage drops out of focus the tension on the stage must be adjusted. To fix
look on the coarse focus arm on the left side of the microscope there are 4 little holes. Take the wrench that is supplied and
attach it to any to of the holes. Then give a quarter turn clock-wise. Tighten only enough to keep stage from drifting down and out
of focus.
Coarse focus is very hard to turn
If the coarse focus is very hard to turn, take the wrench that is supplied and attach it to the tension adjustment holes behind the
focus knob and turn until the tension for focusing is how you like it.
Objectives
If you are using any type of liquid on the slides and then view through the microscope, make sure that you clean the objectives
immediately after you are done. You can clean with paper towel and xylene. If you do not clean after contact with liquid the
objective lens ill become very dirty and hinder the viewing quality of the objective.
The Eyepiece
If view is hindered check for fingerprints on the eyepiece or eyepiece lens could be scratched. Do not scratch eyepiece with your
fingernail, as this will remove the optic coating. Only clean outer lens do not attempt to take eyepiece apart. Cleanbyusing acan
of compressed air for dust or breath on lens and wipe with lens paper.
Rack Stop Screw
In the microscope illustration, you see a stage rack stop screw. This screw sets how far up the stage will move. Students have a
tenancy to screw this all the way down. This limits the upper movement of the stage and objects will not come intofocus. Simply
turn the screw back up, the more the screw comes up the higher the stage will come up. Do not turn the screw to far up because
the stage may hit the 40x and 100x objectives.
Slide and Cover Slip
If the slide does not come into focus check to see that the slide is not upside down. Make sure the cover slide is facing up.
Also make sure the slide is not dirty, spots will show up if it is. Also make sure that the cover slip over the specimen is not too
thick, use a 0.17mm thick cover slip over your specimen.
(I) WARRANTY
Walter Products Inc. warrants their Walter brand microscopes to be free from manufacturer defects in materials or
workmanship for the life of the microscope under normal use and service. The warranty does not cover damage resulting from
abuse or misuse, repairs or alterations performed by other than our authorized repair technicians, or damage occurring in
transit. If your microscope is damaged during shipment, please contact the courier immediately to file a claim. We will repair
your microscope should any problems arise at our discretion with the exception of bulbs, batteries, or fuses. Warranty does
not cover lenses that have become inoperable as a result of misuse or lack of normal maintenance. This offer is limited only
to microscopes sold and used in the United States or Canada. Should you have any other questions regarding our warranty,
please contact our customer service department. Please have on hand your original purchase order number if you are calling
to arrange for a return authorization.
Please Note: When arranging for a return authorization whether it is a repair, return or for credit, please contact
the vendor directly from whom you initially purchased your microscope from.