3
CONTENTS
1 INTRODUCTION............................................................................................................................................................................. 4
2 CONDITIONS OF SERVICE........................................................................................................................................................... 4
3 PRINCIPLE OF OPERATION......................................................................................................................................................... 5
4 SPECIFICATIONS........................................................................................................................................................................... 6
5 CONTROLS......................................................................................................................................................................................... 8
6 CUVETTE CHAMBER .................................................................................................................................................................... 9
7 INSTALLATION & START-UP...................................................................................................................................................... 10
8 GENERAL OPERATIONS............................................................................................................................................................. 12
8.1 REFERENCING...........................................................................................................................................................................12
8.2 MEASUREMENT AT LOW LEVELS (<0.050A).................................................................................................................................12
8.3 DILUTION .................................................................................................................................................................................. 12
8.4 BACKGROUND ABSORBANCE CORRECTION .................................................................................................................................13
9 SOFTWARE OPERATION............................................................................................................................................................ 14
9.1 NUCLEIC ACIDS.........................................................................................................................................................................14
9.1.1 dsDNA, ssDNA, RNA.................................................................................................................................................14
9.2 PROTEIN METHODS ...................................................................................................................................................................16
9.2.1 Colorimetric Protein methods..................................................................................................................................... 16
9.2.2 Direct UV Absorbance determination of Proteins ...................................................................................................... 20
9.3 CELL DENSITY MEASUREMENT....................................................................................................................................................21
9.4 OTHER......................................................................................................................................................................................22
9.5 SINGLE/MULTI λ........................................................................................................................................................................22
9.5.1 Single measurement................................................................................................................................................... 22
9.5.2 Multi Wavelength........................................................................................................................................................ 23
9.6 SCANNING.................................................................................................................................................................................24
9.7 OTHER METHODS ...................................................................................................................................................................... 25
9.7.1 To Program a New Method (or re-program an existing method)............................................................................... 25
9.7.2 Run a Method.............................................................................................................................................................31
10 INSTRUMENT SET-UP ................................................................................................................................................................ 33
10.1 SET CONSTANTS.................................................................................................................................................................33
10.2 COMMUNICATIONS...............................................................................................................................................................34
10.3 SET TIME/DATE................................................................................................................................................................... 35
10.4 AUTO-PRINT....................................................................................................................................................................... 35
11 PRINTING..................................................................................................................................................................................... 36
12 CONNECTION TO PC .................................................................................................................................................................. 37
12.1 SETTING UP THE BIOWAVE TO A PC LINK .............................................................................................................................. 37
12.2 USING THE BIOCHROM DATA CAPTURE SPREADSHEET INTERFACE SOFTWARE
(80-2112-23).............................................................................................................................................................................37
13 SERVICING AND MAINTENANCE............................................................................................................................................... 38
13.1 REPLACING THE LAMPS........................................................................................................................................................38
13.2 ROUTINE MAINTENANCE ...................................................................................................................................................... 39
13.3 DECONTAMINATION .............................................................................................................................................................39
13.4 CALIBRATION ...................................................................................................................................................................... 39
14 CE MARKING ............................................................................................................................................................................... 40
15 GUARANTEE................................................................................................................................................................................ 40
16 HEALTH & SAFETY CERTIFICATE AND DECLARATION OF DECONTAMINATION STATUS ............................................... 41
APPENDIX 1 – CALCULATIONS.............................................................................................................................................................. 42
NUCLEIC ACIDS......................................................................................................................................................................................42
PROTEIN UVABSORBANCE METHOD .......................................................................................................................................................43
BACKGROUND ABSORBANCE .................................................................................................................................................................. 43
16.1 OTHER METHODS ................................................................................................................................................................43
APPENDIX 2 -CONNECTING TO HYPERTERMINAL AND DOWNLOADING RESULTS...................................................................... 44
