WPI EVOM EVA-MT-03 Series User manual

WORLD
PRECISION
INSTRUMENTS
EVA-MT-03-XX
EVOM™ Auto Automated TEER Measurement System
with 24 & 96 Capability (v. 3)
Serial No._____________________
www.wpiinc.com
INSTALL GUIDE
092823

EVA-MT-03
World Precision Instruments i
Copyright © 2023 by World Precision Instruments. All rights reserved. No part of this publication may
be reproduced or translated into any language, in any form, without prior written permission of World
Precision Instruments, Inc.
CONTENTS
ABOUT THIS MANUAL ................................................................................................................... 1
INTRODUCTION .............................................................................................................................. 2
Features....................................................................................................................................... 2
Notes and Warnings:................................................................................................................ 3
Parts List...................................................................................................................................... 3
Unpacking................................................................................................................................... 4
INSTRUMENT DESCRIPTION ........................................................................................................ 5
EVOM™ Auto System................................................................................................................ 5
Autosampler............................................................................................................................... 6
Interface Unit ............................................................................................................................. 7
SYSTEM SETUP ................................................................................................................................ 8
Setting up the System.............................................................................................................. 8
Installing the Electrode Array Head....................................................................................11
Starting the System ................................................................................................................14
Lost Connection ......................................................................................................................17
Installing the Rinse Stations ................................................................................................18
Installing the Well Plates........................................................................................................19
Selecting a Plate Brand and Plate Prole..........................................................................20
Verifying Alignment.................................................................................................................21
APPENDIX A: USING EXPERT MODE TO POSITION WELL PLATE.......................................25
Saving a Prole Setting ..........................................................................................................26
Emergency Stop ......................................................................................................................26
Well Plate Adjustments..........................................................................................................27
Adjusting the Electrode Array Position..............................................................................28
APPENDIX B: USING AN ETHERNET CONNECTION ..............................................................32
APPENDIX C: RESTORE FACTORY DEFAULTS .........................................................................32
APPENDIX D: ADDING LINK TO IPAD HOME SCREEN..........................................................35
DECLARATION OF CONFORMITY ..............................................................................................38
WARRANTY .....................................................................................................................................39
Claims and Returns ................................................................................................................39
Repairs.......................................................................................................................................39

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ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can cause
damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can cause
personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1—The EVOM™ Auto with 96 high throughput screening (HTS) electrode array simplies
transepithelial electrical resistance (TEER) measurement in 96-well HTS plates from MatTek,
Corning, and Millipore multiwell plates. The system is also available for 24-well plates.
The EVOM™ Auto automates measurements of TEER in epithelial or endothelial
monolayers cultured on 24-well or 96-well high throughput screening (HTS) plates
utilizing our innovative EVOM technology, qualitatively measuring cell monolayer
health and quantitatively measuring cellular conuence by reaching an increase or a
plateau in tissue resistance.
Automated measurement of tissue resistance in cell culture microplates provides the
advantages of speed and precision, minimizes the chances of contamination, and
ensures the rapid availability of measured resistance data. EVOM™ Auto applies a
12.5 Hz square wave current with switching polarity, which mitigates the chances of
charging the sample and nullies the chances of measurement technique having any
adverse eects on the sample. Therefore, it is considered non-invasive measurement.
EVOM™ Auto is designed for the non-destructive, high throughput screening of
epithelial monolayer conuence and barrier function studies in cell cultures.

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INTRODUCTION
The EVOM™ Auto automates TEER measurements. The instrument is controlled
through a web browser, and the measurements are stored in the instrument and
downloaded to your computer or iPad (control device) through the web browser. At
present, the instrument supports MatTek , Corning, and Millipore 96 HTS well plates
using the 96-well electrode array, Corning 24 HTS plates using the 24C electrode
array, and Millipore 24 HTS plates using the 24M electrode array. EVOM™ Auto is
controlled wirelessly via Wi-Fi connectivity using an iPad (included with the system) or
any other device with Wi-Fi connectivity.
Features
Features Advantages Benets
Electrodes
available for 24-
and 96-well HTS
plates
Measures wells one column at
a time
Eciency gains with automation
Easily switch
between 24 and
96 plate types
Plug-n-play enables fast, easy
switching between plate types
and heads
Multi-plate compatibility utilzing
only one instrument means cost
and space savings
Automate your
measurements
Streamline your workow Minimize human errors
Three rinse
locations
Rinse electrodes multiple times
during a measuring sequence
Take control of protocol and dene
custom sequences
Crash protection Auto alignment detction pauses
measurements, preventing
equipment damage
Minimize electrode damage,
avoiding costly repairs and down
time
Auto-detection of
electrode head
Auto electrode detection
congures its position and
software options for your plate
Simple hardware setup without
conguration
Intuitive
touchscreen user
interface
User-friendly programming
sequences with basic selection
options
Easy-to-navigate system saves time
when conguring sequences
Create custom
plate prole
Adjust the programmed
coordinates
Fine tune the programming as
desired
Save up to three
plate proles per
plate type
Multiple users can operate
instrument with individually
saved settings
Ensures custom settings are
saved, preserving data integrity
Store all your
data or export to
Microsoft®Excel
Analyze your data in a manner
suitable to your workow
Flexibility to manage your data

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Notes and Warnings:
CAUTION: Never set anything (especially liquids) on top of the autosampler.
CAUTION: When you move or relocate the instrument, remove the front
cover/lid, which can fall o. For example, you may move the system in and out
of a cell culture hood. Always remove the EVOM™ Auto’s front cover anytime
the system is lifted and moved from one location in the lab to another and reinstall
the cover after the system has been placed at a new location.
CAUTION: To safely change the electrode array, the software must be in oine
mode OR the unit must be powered o. If one of these conditions is not met,
DO NOT try to change the electrode array. Removing the electrode array when
the system is in the fully active mode may put the system in an unresponsive,
disrupted communication mode that may necessitate a return to the factory.
TIP: Before you begin experimenting, verify the time zone setting is set correctly on
your iPad control tablet. For instructions, visit the support.apple.com page.
Parts List
After unpacking, verify that there is no visible damage to the equipment. Verify that all
items are included:
(1) Autosampler
(1) Electrode Array Kit for 96 HTS plates (Corning, Millipore, and MatTek), 24 HTS
Corning plates, or 24 HTS Millipore plates.* The kit includes all the required
matching accessories for the selected electrode array, as follows:
•EVA-EL-03-01 EVOM™ Auto 96 HTS Electrode Array for TEER includes:
(1) Electrode Array
(3) Depth adjustment xtures (C96 for Corning 96, M96 for Millipore 96, and
MT96 for MatTek 96)
(4) Plate Positioners (2 C96/MT96 for Corning and MatTek 96 HTS plates,
and 2 M96 for Millipore 96 HTS plates)
•EVA-EL-03-02 EVOM™ Auto 24C HTS Electrode Array for TEER includes:
(1) Electrode Array
(1) Depth adjustment xtures (C24 for Corning 24)
(2) Plate Positioners (2 C24 for Corning and Millipore 24 HTS plates)
•EVA-EL-03-03 EVOM™ Auto 24M HTS Electrode Array for TEER includes:
(1) Electrode Array
(1) Depth adjustment xtures (M24 for Millipore 24)
(2) Plate Positioners (2 C24 for Corning and Millipore 24 HTS plates)
(1) iPad Generation 9 Control Tablet

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(1) Interface Unit
(1) Interface Unit Cable
(1) Power Supply Unit
(2) Wi-Fi Adaptor
(1) Instruction Manual is available online at www.wpiinc.com/manuals.
* The system includes one electrode array and its accessories. Other electrode arrays
are sold separately. The electrode array is chosen when ordering. Options include 96-
well electrode array, Corning 24-well electrode array, and Millipore 24-well electrode
array.
Unpacking
Upon receipt of this instrument, make a thorough inspection of the contents and
check for possible damage. Missing cartons or obvious damage to cartons should be
noted on the delivery receipt before signing. Concealed damage should be reported
at once to the carrier and an inspection requested. Please read the section entitled
“Claims and Returns” on page 39 of this manual. Please contact WPI Customer
Service if any parts are missing at [phone] or [email protected].
Returns: Do not return any goods to WPI without obtaining prior approval (RMA
# required) and instructions from WPI’s Returns Department. Goods returned
(unauthorized) by collect freight may be refused. If a return shipment is necessary,
use the original container, if possible. If the original container is not available, use a
suitable substitute that is rigid and of adequate size. Wrap the instrument in paper or
plastic surrounded with at least 100mm (four inches) of shock absorbing material. For
further details, please read the section entitled “Claims and Returns” on page 39 of
this manual.
NOTE: Before shipping your system back to WPI, please contact [email protected]
for assistance to ensure proper packaging and avoid damage during shipping.

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INSTRUMENT DESCRIPTION
EVOM™ Auto System
Fig. 2—The EVOM™ Auto System, includes the iPad, the autosampler, the interface unit and
the power supply (not shown).
The EVOM™ Auto consists of these major components:
•Autosampler – The robot contains the electrodes, a 3-compartment rinse
stations, and a well plate area. The autosampler may be placed inside an
incubator for long term studies, if desired. It may also be used inside a cell culture
laminar hood.
•Interface Unit – This unit connects through a cable to the autosampler. This unit
is a Wi-Fi access point to which the tablet (laptop or iPad) connects to interface
with the instrument.
•Power Supply – The power supply provides power to the autosampler and the
interface unit. The cable plugs into the interface unit power supply port and a
standard wall outlet.
•iPad – The tablet with Wi-Fi capabilities runs a web browser to communicate with
the system.
•Wi-Fi Adapter – The Wi-Fi adapter plugs into one of the four USB ports on the
right side of the interface unit. It has a range of at least 7 m (25 ft.), and it allows
the iPad to communicate with the interface unit.

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Autosampler
Fig. 3—The autosampler takes the TEER measurements of the HTS well plate.
Here are the primary components of the autosampler.
Electrode Array Head – The electrode array rests in the home position (shown in Fig.
3), just to the left of the rinse stations. The LED on the electrode array will:
• Flash blue when good measurements are being made.
• Be a steady red on power up. This shows the unit has power but is not yet in
communication with the interface unit.
•Blink red when a bad measurement is made. (See “Online/Oine Toggle” on
page 9.)
• Be a steady blue when it is in communication with the interface unit.
Well Plate Area – The HTS well plates are positioned in the well plate area.
Rinse Stations – Three rinse stations are located on the left side of the autosampler.
These may be used for stabilizing, cleaning or chloridizing.
Rinse Station 1
Rinse Station 2
Rinse Station 3
Interface Unit Connection Port – One end of the autosampler cable connects
here and the other end connects to the interface unit. The autosampler power and
communications travel through this cable.

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Looking at the autosampler measurement plate from the top, you can see the:
• Rinse Station Pocket where the rinse station slides into place.
•Plate Positioner Sockets for installing the Plate Positioner used for positioning
the well plate precisely.
• Spring loaded Well Plate Handle used to hold the well plate in place.
• Thumb Well for ease of grasping a well plate.
Fig. 4—Top view of the measurement plate on the autosampler
Interface Unit
Fig. 5—(Left) Side view of the interface unit, showing the front.
Fig. 6—(Right) Back view of the interface unit.
Power Button – Press the power button to turn on the interface unit and the
autosampler. When it is powered on, a blue ring illuminates the button.
Autosampler Cable –This cable plugs into the autosampler cable connector on
the interface unit and the interface unit connection port on the left side of the
autosampler.
Interface Unit Power Supply Port – Plug the power supply cable into this port.
Ethernet Connection Port – This port may be used to connect to your local area
network through a standard ethernet cable. Using this option requires interfacing with
your local network and observing all your IT policies.
USB Ports (4) – Use one of these ports to connect the Wi-Fi adapter.

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SYSTEM SETUP
The autosampler is packed with the lid taped in place. Remove all the components
from the packing materials and remove the protective tape.
CAUTION: When you move or relocate the instrument, remove the front
cover/lid, which can fall o. For example, you may move the system in and out
of a cell culture hood. Always remove the EVOM™ Auto’s front cover anytime
the system is lifted and moved from one location in the lab to another and reinstall
the cover after the system has been placed at a new location.
Setting up the System
1. Remove the safety tape from the lid and remove the lid from the autosampler.
2. Connect the interface unit to the autosampler using the interface cable. Connect
one end to the interface unit connection port on the autosampler (Fig. 7) and
connect the other end to the autosampler cable connection port on the interface
unit (Fig. 8). Line up the pins on the connector with the port and press the
connector rmly into place. Rotate the knurled ring to secure the cable.
Fig. 7—Line up the pins and connect the interface cable to the autosampler.
Fig. 8—Line up the pins and connect the interface cable to the interface unit.

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3. Connect the power supply wall adapter to the wall outlet and insert the barrel
connector into the interface unit power supply port on the back side of the
interface unit.
Fig. 9—Connect the power cable to the interface unit.
4. Install the provided Wi-Fi adapter into any one of the four USB ports on the back
of the interface unit.
Fig. 10—The Wi-Fi adapter is installed in one of the four USB ports on the back of the
interface unit.
NOTE: You must install the electrode array before powering on the system.

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5. The autosampler ships with a red electrode array lock in place (Fig. 11). To
remove it:
• Pinch the lock at the top (Fig. 12) and lift the slide on the side (Fig. 13).
• Push the locking mechanism towards the right till the clasp releases from the
slot in the base plate. (Fig. 14)
• Firmly grasp the electrode port cap and pull it out of the port socket. (Fig. 15)
Fig. 11—The autosampler ships with a red lock to prevent the electrode array connection
port from moving during transit.
Fig. 12—(Left) Pinch the latch at the top of the lock.
Fig. 13—(Right) Release the slide from the slot in the base and push it up so it moves freely.
Fig. 14—(Left) Push the locking mechanism till the clasp releases from the slot in the base.
Fig. 15—(Right) Pull the electrode port cap out of the port socket.

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6. Make sure that there are no well plates installed on the autosampler and make
sure that the rinse station block is not installed on the autosampler plate.
Rinse
Station
Pocket
Electrode Array Connection Port
Well Plate Area
Fig. 16—This autosampler has no well plate and no rinse station installed. When the lock is
removed, you have access to the electrode array connection port.
CAUTION: Never set anything (especially liquids) on top of the autosampler.
Installing the Electrode Array Head
1. Locate the electrode array box and carefully remove the electrode array. It is
shipped with a red protective sleeve to prevent damage to the electrode tips.
Fig. 17— The electrode array is packaged with a protective sleeve secured with a heavy
Velcro strap.

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2. Remove the red electrode protection shield by releasing the Velcro strap.
Fig. 18—Remove the protective sleeve.
3. Make sure that the autosampler is in the home position on the left side of the
unit.
4. Locate the electrode array connection port on the back wall of the autosampler.
Fig. 19—The electrode array connection port is on the back wall of the autosampler.
5. Place the electrode array in front of the connection port and line it up. Hold the
top left corner of the autosampler with your left hand. With the electrode array
in your right hand and with the electrode tips pointing down, line it up with the
connection port. Press rmly until it stops and you hear a click (Fig. 20). Then give
it a little tug (without pushing the button on the side) to ensure it says in position.
This helps with alignment of the electrode with the plate.
NOTE: To remove the electrode array, depress the button on the left side of the
array with your thumb and pull it straight out of the connection port. (Fig. 21)

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CAUTION: To safely change the electrode array, the software must be in oine
mode OR the unit must be powered o. If one of these conditions is not met,
DO NOT try to change the electrode array. Removing the electrode array when
the system is in the fully active mode may put the system in an unresponsive,
disrupted communication mode that may necessitate a return to the factory.
NOTE: The electrode array needs to be installed before trying to power up the
unit, otherwise the software will not recognize the device and will not connect.
Fig. 20—Hold the top of the autosampler with one hand, line up the electrode array with
the connection port and press the electrode array into the connection port until it clicks.
Fig. 21—Depress the button on the left side of the electrode array until you hear a click.
Then, pull the array out to remove the electrode array. No light on the electrode array
indicates the unit is o, and a red light on the electrode array indicates you are in oine
mode. In either case, it is safe to remove the electrode array.

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Starting the System
NOTE: Before you begin using the iPad, your IT department should register the
device so that it is properly licensed to the organization. An iCloud account should be
established to upload les. When you receive it, the iPad is congured to begin using
the autosampler, but WPI recommends that you register it to your organization for
optimal use of the device.
NOTE: On the iPad, the screen timeout is set to the maximum. If you are planning
to use a dierent device to operate the EVOM™ Auto, set the screen timeout to the
maximum. If the device screen turns o or goes into the sleep mode, it will disrupt the
web browser connectivity with the autosampler. In this case, the web-browser window
needs to be closed and launched each time to reestablish the connection
1. Power on the system by pressing the power button on the interface unit. Make
sure the LED in the electrode array head is lit red (Fig. 19). The blue ring around
the button on the interface unit illuminates (Fig. 23).
Fig. 22—The electrode array LED is illuminated red.
Fig. 23—The blue ring illuminates around the power button when the unit is powered on.
2. Allow the system to boot up. On the iPad, navigate to the Setting area and select
Wi-Fi. Select your EVOM system when it appears as an available network on
the list of Wi-Fi networks. (This may take up to 5 minutes. The electrode array
will move to its home position as a self test during this time to establish the
connection). The network will be named EVOM_AUTO_X where X is unique to the
system. The password is rems2rems2.

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3. If there is more than one EVOM™ Auto systems being used with the iPad, you
will need to go to the Wi-Fi settings on the iPad to select your device in order
to establish a connection. Sometimes the bookmarked URL may not work to
establish a connection. If a connection is unsuccessful, ensure that the iPad is
connected to the correct Wi-Fi network for your EVOM™ Auto. Then, click on the
WPI icon (Fig. 26). If you are not using the provided iPad tablet, open a browser
and navigate to https://192.168.54.1:5000.
NOTE: If you are using more than one EVOM™ Auto simultaneously, you may
need to go to the Wi-Fi settings and select the appropriate device.
Fig. 24—EVOM Auto icon located on the iPad’s desktop.
NOTE: You may use a computer browser to access the software. Once you are
connected, open your browser and enter the URL 192.168.54.1:5000.
TIP: Add an icon to your iPad home screen to open the application in full screen
mode. See “Appendix D: Adding Link to iPad Home Screen” on page 35.
4. The Initializing window displays (Fig. 25) showing the progress. The window briey
displays a ready message when the initialization is complete (Fig. 26). Then, the
Main window appears (Fig. 27).
Fig. 25—Initialization screen.
Fig. 26—Ready message displays briey.

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Fig. 27—Main window.
This is your main window. Keep the online slider enabled to perform experiments.
• The Experiment menu to select a sequence and start an experiment.
• The Manage Sequence menu to create or modify a sequence.
• The Expert menu lets you adjust the plate electrode coordinates (positioning).
• The Maintenance menu lets you perform electrode maintenance or cleaning, like
chlorodizing or disinfection.