WPI EVOM3 User manual

WORLD
PRECISION
INSTRUMENTS
EVOM3
Epithelial Volt/Ohm Meter
Serial No._____________________
www.wpiinc.com
INSTRUCTION MANUAL
121520

EVOM3
World Precision Instruments i
Copyright © 2020 by World Precision Instruments. All rights reserved. No part of this publication may
be reproduced or translated into any language, in any form, without prior written permission of World
Precision Instruments, Inc.
CONTENTS
QUICK START ................................................................................................................................... 1
Resistance................................................................................................................................... 1
Voltage......................................................................................................................................... 1
TEER Calculations...................................................................................................................... 2
ABOUT THIS MANUAL ................................................................................................................... 3
INTRODUCTION .............................................................................................................................. 3
EVOM3 Features ....................................................................................................................... 4
EVOM3 Benets......................................................................................................................... 4
STX2-PLUS Features................................................................................................................. 5
STX2-PLUS Benets .................................................................................................................. 5
Notes and Warnings................................................................................................................. 5
Tips and Advice.......................................................................................................................... 6
PARTS LIST........................................................................................................................................ 7
Unpacking................................................................................................................................... 8
INSTRUMENT DESCRIPTION ........................................................................................................ 9
EVOM3 Meter............................................................................................................................. 9
STX2-PLUS Electrodes.............................................................................................................. 9
Theory of Operation...............................................................................................................10
Main Display.............................................................................................................................10
Setup Page................................................................................................................................11
Plate Selection Page and Plate Preview.............................................................................11
Mode Units ...............................................................................................................................12
Blank Handling Page ..............................................................................................................13
Setting the Date and Time....................................................................................................13
Probe Null.................................................................................................................................14
Storing Data..............................................................................................................................15
Viewing Data in Microsoft Excel or Other Spreadsheet Program...............................16
USB Testing ..............................................................................................................................17
SYSTEM MESSAGES......................................................................................................................18
Last Cell Warning ....................................................................................................................18
Power Level Messages...........................................................................................................18
Reloading a Backup File after Power Failure....................................................................20
OPERATIONS..................................................................................................................................21
Instrument Diagnostics and Calibration............................................................................21
Making Resistance Measurements.....................................................................................22
Making Voltage Measurements...........................................................................................24

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MAINTENANCE ..............................................................................................................................26
Cleaning/Maintaining the STX2-PLUS Electrodes............................................................26
Disinfecting the STX2-PLUS Electrodes .............................................................................26
Sterilizing the STX2-PLUS Electrode...................................................................................27
Rechloriding the Electrodes .................................................................................................27
Storing the Electrodes ...........................................................................................................28
Testing the STX2-PLUS Electrodes......................................................................................28
ACCESSORIES.................................................................................................................................29
TROUBLESHOOTING ...................................................................................................................31
SPECIFICATIONS............................................................................................................................35
APPENDIX A: KCL TESTING MIXTURES.....................................................................................36
APPENDIX B: FILE FORMATS ......................................................................................................36
Resistance (*.CSV)..................................................................................................................36
Voltage (*.csv) ..........................................................................................................................36
APPENDIX C: CONTINUOUS RECORDING VIA USB (PYTHON) ON A PC..........................36
Running the External Data Connection Output Program (PC, Mac, Linux) .............. 37
Sample CSV recording ...........................................................................................................39
APPENDIX D: 24 MM DIAMETER (6-WELL) INSERTS .............................................................40
APPENDIX E: IMPROVING THE ACCURACY AND REPEATABILITY OF THE SYSTEM ....... 40
APPENDIX F: RESISTANCE CALCULATIONS.............................................................................42
Resistance.................................................................................................................................42
Resistance Value of the “Blank” Insert ...............................................................................42
Unit Area Resistance ..............................................................................................................43
TEER Calculations....................................................................................................................44
APPENDIX G: FREQUENTLY ASKED QUESTIONS...................................................................44
DECLARATION OF CONFORMITY ..............................................................................................45
WARRANTY .....................................................................................................................................47
Claims and Returns ................................................................................................................47
Repairs.......................................................................................................................................47

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QUICK START
Resistance
1. Power on the EVOM3 using the power switch on the rear panel.
2. If a lename other than the default (plate1) is required:
• Press and hold the Store button for 2 seconds to enter the Store Option menu.
• Select Filename to enter a new name.
• Press Clear .
• Use the on screen keypad to enter a new name.
• Press Enter .
NOTE: Auto indexing changes the name to a numeric sequence formatted with
the name, a prex and a sequential number (name_prexN).
3. Press
Setup to access the Setup menu.
• Select Mode–Set the mode to Resistance.
• Select Plate–Choose the plate size.
• Display Units– Select the display units in ohms or K-ohms.
• Resistance–Select the resistance range (auto or the desired range).
• Calibrate–Press Calibrate to calibrate the electrode. (This uses a 10K Ω
resistor to calibrate the cell ranges).
4. Press
Return to navigate back to the main menu.
5. Insert a USB thumb drive into the USB port on the side of the EVOM3 and begin
measuring by pressing the foot switch or touching the
Store button on the screen
to store and advance to the next weel. Tap the Next Well area on the right side of
the screen to advance in increments of one well without taking a measurement.
Repeat the sampling process.
6. Once the last well has been recorded, the le storing selection notication
appears. Press
Store . A system message will report if the USB thumb drive is
usable, or it will display an error. If the USB thumb drive is not plugged in or it
is not recognized, a warning message will appear. Press Cancel and try another
drive. The Store function cannot be used on an unrecognized drive. EVOM3
should respond with “File saved” within 15 seconds. If “Saving le...” is still seen
after 20 seconds, press the Return key and use another USB drive. Once a le has
been saved, open the le on a computer to verify the contents.
NOTE: The le saving routine can take up to 15 seconds to complete. See the
USB cautions on page 5.
7. Remove the USB thumb drive and transfer the data le to your computer as a
CSV le (Excel).
Voltage
1. Power on the EVOM3 using the power switch on the rear panel.
2. If a lename other than the default (plate1) is required:
• Press and hold the Store button for 2 seconds to enter the Store Option menu.

2 World Precision Instruments
• Select
Filename to enter a new name.
• Press Clear .
• Use the on screen keypad to enter a new name.
• Press Enter .
NOTE: Auto indexing changes the name to a numeric sequence formatted with
the name, a prex and a sequential number (name_prexN).
3. Press Setup to access the Setup menu.
• Select Mode–Set the mode to PD.
• Select Plate–Choose the plate size.
• Display Units– Select the display units in volts or millivolts. (The normal mode
is millivolts.)
• Calibrate (zero) the electrodes in saline. Press the Probe Null button. Push the
Null button. Wait for the second message stating ”Solution value 0.0” before
returning. This action removes any voltage osets on the electrodes.
• Press Return to navigate back to the main menu.
4. Insert a USB thumb drive into the USB port on the side of the EVOM3 and begin
measuring by pressing the foot switch or touching the
Store button on the screen.
Tap the Next Well area on the right side of the screen to advance to the next well.
Repeat the sampling process.
5. Once the last well has been recorded, the le storing selection notication
appears. Press
Store . A system message will report if the USB thumb drive is
usable, or it will display an error. If the USB thumb drive is not plugged in or it
is not recognized, a warning message will appear. Press Cancel and try another
drive. The Store function cannot be used on an unrecognized drive. EVOM3
should respond with “File saved” within 15 seconds. If “Saving le...” is still seen
after 20 seconds, press the Return key and use another USB drive. Once a le has
been saved, open the le on a computer to verify the contents.
NOTE: The le saving routine can take up to 15 seconds to complete. See the
USB cautions on page 5.
6. Remove the USB thumb drive to transfer the data le to your computer as a CSV
le (Excel).
TEER Calculations
To compute TEER, multiply the measured resistance by the surface area’s listed below.
For example, a 12 mm insert measures 565 Ω, the TEER is 565Ω * 1.13 or 638 Ω.
• 6 well plate (24 mm inserts) 4.52 cm2
• 12 well plate (12 mm inserts) 1.13 cm2
• 24 well plate 6.5 mm inserts) 0.3316 cm2
• 96 well plate (4.3 mm inserts) 0.145 cm2

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ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can cause
damage to equipment or data. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can cause
personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1—EVOM3
INTRODUCTION
EVOM3 is the WPI’s newest instrument to measure Trans Epithelial Electrical
Resistance (TEER) measurement. The EVOM3 has been re-engineered to facilitate
ease of accurate, repeatable measurements and transfer of data. You no longer need
to log data by hand. The resistance or voltage information is written to a USB drive
in a format (CSV) that can be opened by most spreadsheet programs on either PC or
Mac. The processor controlled instrument is more stable and accurate. Included is a
footswitch for quick hands free recording to the USB drive. The touch screen interface
is easy to use and setup for setting control wells in resistance or voltage modes
and measurements. The auto ranging resistance feature allows for fast resistance
measurements, and an overrange display feature eliminates false readings. The
EVOM3 now has adjustable current levels in three xed ranges with two lower ranges
for sensitive membranes and high resistance ranges up to 100K Ω.
The STX2-PLUS electrode was redesigned for easier insertion into and 24-well
plates and to be location re-placeable in the insert for repeatable and consistent
measurements. The new shielded electrodes are now designed to minimize electrical
interference and to be more easily maintained.
Conuence of a cellular monolayer is determined by an increase or a plateau in tissue
resistance detected using the unique electronic circuit of the EVOM3 and the new

4 World Precision Instruments
STX2-PLUS electrode. The EVOM3 qualitatively measures cell monolayer health and
quantitatively measures cell conuence. The EVOM3 produces a low AC current that
avoids electrode metal deposits and adverse eects on tissues which can otherwise
be caused by higher DC currents. The EVOM3 uses low current and voltages and
is designed for non-destructive testing for epithelial monolayer conuence in cell
cultures. In addition, resistance readings are unaected by membrane capacitance or
membrane voltage. The accuracy and repeatability of the EVOM3-STX2-PLUS system
makes this instrument ideal for permeability, PD and other detailed membrane studies.
EVOM3 Features
• Low noise design oers greater resolution and accuracy
• Automatic 20X sample averaging improves accuracy and stability
• Adjustable xed measurement currents (2, 4 or 10 μA)
• Resistance auto ranging from 1 Ω to 100,000 Ω or with three xed current ranges
• Reliable low current, low voltage design prevents metal ion transport
• Fast resistance stabilization on low levels under 200 Ω with resolution to 0.1 Ω
• Ergonomic tilt stand for low glare operation
• Graphical display of popular plates (6, 12, 24, 96) for trend analysis
• The display shows the most recent set of parameters
• Automatic plate indexing operation with or without control well subtraction for
resistance and potential dierence (PD) measurements
• Continuous data logging via USB (PC, Mac, Linux)
• Saves date stamped data to a spreadsheet readable le on a USB drive
• Upgradable rmware
EVOM3 Benets
Eliminates errors and reduces experimental processing time
Auto data logging eliminates the need to track data by hand
The small footprint allows more bench space
Easy calibration and verication
Footswitch for hands-free recording
Prevent data loss with auto save and data recovery when battery
is low
TEER is easily computed by applying a unit area formula to the
resistance

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STX2-PLUS Features
• STX2-PLUS new electrode designed for 12 and 24-well plates.
• Weighted self-standing electrode for hands free stable measurements
• Shielded cable to minimize electrical and cell phone interference
STX2-PLUS Benets
• Keyed electrode base for repeatable placement gives more consistent results
eliminating the need for multiple readings.
• Easy to maintain
Notes and Warnings
Choice of current/range: The EVOM3 has three ranges of current from 2 µA to
10µA and each current range has its own resistive upper limit up to 100K Ω:
• 10K Ω range = 10 µA
• 50K Ω range = 4 µA
• 100K Ω range = 2 µA
The auto range will adjust the current as needed from 2 µA to 10 µA depending on
the resistance.
General rules
• The higher the current the greater the stability of the measurement. The xed
ranges may be more consistent with a xed current for some cell lines.
• If a cell line is not expected to read above 10K Ω, then choose that xed range.
Use of the charger: The EVOM3's Li-ion battery will last 8 hours on its own. The
charging time is 5.5 hours with the unit powered o. The EVOM3 charger should not
be plugged in all the time. The chager may be used to power the EVOM3 for normal
and long-term use.
NOTE: In the higher resistance ranges (50K -100K ) there may be a slight instability
added from the noise of the power supply, in that case unplug it during those
measurements.
USB Drives: WPI has supplied a tested USB thumb drive. Most drives of 32 Gb
(USB2.0) or smaller sizes have been tested.
CAUTION: Not all USB drives work on the EVOM3.
To test a USB drive, see "Sample Resistance File (*.CSV)" on page 16. This test saves
a le to the drive and reads it back. A system message will report if the USB thumb
drive is usable, or it will display an error. If the USB thumb drive is not plugged in or
it is not recognized, a warning message will appear. Press
Cancel and try again. The
Store function cannot be used on an unrecognized drive. EVOM3 should respond with
“File saved” within 15 seconds. If “Saving le...” is still seen after 20 seconds, press the
Return key and use another USB thumb drive. Once a le has been saved, open the
le on a computer to verify the contents.

6 World Precision Instruments
Tips and Advice
Out of Range Error
If you see –––– (5 dashes) on the main resistance display, then the resistance reading
is out of range. Check the ohms range setting to see if it can be increased to display a
higher resistance or set it to Auto . If the display still shows ––––, then go to the Blank
Handling screen and check the settings and the reading. A saved reading will show a
space (“ “) in the grid if the display shows out of range. Do not forget to reset the blank
to zero or go back to a control well resistance when you are nished.
NOTE: The 100K Range can read 5% over to 105K Ω.
Zeroing the blank handling value
1. Select Setup .
2. Then, press
Short Electrodes .
3. Press the
Blank Handling button.
4. Set the reading to zero.
5. Return to the
Setup page and open the electrodes to remove the short.
Zeroing the probe null oset
1. Select Setup .
2. Then, press
Short Electrodes .
3. Press the
Probe Null button.
4. Set the reading to zero.
5. Return to the
Setup page and open the electrodes to remove the short.
Resistance levels that are unexpectedly lower or higher can be attributed to a number
of things besides an incompletely grown membrane. Any of these items can change
the resistance reading:
NOTE: Many of these conditions are eliminated with the STX2-PLUS electrodes.
• Electrode resistance (Keep the electrodes clean and chlorided.)
• Fluid levels (Try to maintain the same volume for every insert. A variation of 50µL
can have a large eect in a small insert.)
• Changing CO2levels and temperature drift can account for large changes in the
resistance reading. The use of a warming plate can reduce this eect.
• We recommend that both uid levels are at the same height so that you do
not have any pressure dierentials and the apical well is lled rst so that you
do not dislodge the membrane from the lter by hydrostatic pressures. If the
membrane detaches itself, then it was not adhering to the inserts lter. A small
gap from dislodged tissue at the edge of the membrane can open a clear path for
the EVOM3 to read a lower resistance. Instead of the current going through the
membrane, the electricity is going around the membrane. Electricity will take the
path of least resistance and the EVOM3 will measure that as a lower resistance.

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NOTE: Cleaning the electrode often corrects most of these issues. See "Maintenance"
on page 26.
In the STX2-PLUS manual is a KCl molarity test. This test will demostrate electode
functionality.
Charging the EVOM3
The EVOM3 battery may also be charged with a Mini-B USB cable P/N 803026 (not
supplied).
Parts List
After unpacking, verify that there is no visible damage to the sensor. Verify that all
items are included:
(1) EVOM3 Epithelial Volt Ohm Meter
(1) STX2-Plus Electrode set
(1) 300749 USB drive 32 GB (For storage and this contains a Python 3.8 program for
continuous digital monitoring of a target insert. See page 36.)
(1) 503535 USB cable
(1) 99673 1000Ω Test Resistor
(1) 803025 A/C power cord and charger
(1) 13142 Foot switch
Instruction Manuals for the EVOM3 and STX2-Plus electrode can be downloaded at
https://www.wpiinc.com/manuals.
13142
803025
503535
STX2-PLUS
300749
EVOM3 99673
Fig. 2—The EVOM3 includes these parts.
NOTE: An 99672 EVOM2 to EVOM3 Electrode Adapter is sold separately. The
STX2, STX3 and all STX100s require the use of this adaptor with the EVOM3. See
"Accessories" on page 29.

8 World Precision Instruments
Unpacking
Upon receipt of this instrument, make a thorough inspection of the contents and
check for possible damage. Missing cartons or obvious damage to cartons should be
noted on the delivery receipt before signing. Concealed damage should be reported
at once to the carrier and an inspection requested. Please read the section entitled
“Claims and Returns” on page 47 of this manual. Please contact WPI Customer
Service if any parts are missing at [phone] or [email protected].
Returns:
Do not return any goods to WPI without obtaining prior approval (RMA
# required) and instructions from WPI’s Returns Department. Goods returned
(unauthorized) by collect freight may be refused. If a return shipment is necessary, use
the original container, if possible. If the original container is not available, use a suitable
substitute that is rigid and of adequate size. Wrap the instrument in paper or plastic
surrounded with at least 100 mm (4”) of shock absorbing material. For further details,
please read the section entitled “Claims and Returns” on page 47 of this manual.

EVOM3
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INSTRUMENT DESCRIPTION
EVOM3 Meter
Fig. 3—USB port, Electrode connection
Fig. 4—Foot switch, Future expansion, reset, power connection, power switch
STX2-PLUS Electrodes
Fig. 5—STX2-PLUS electrode

10 World Precision Instruments
When using the electrode with a 24-well plate, WPI recommends the following uid
levels:
• Apical well – 250 µl
• Basal well – 750-1000 µl (when using a Corning 347x plate).
Place the electrodes into the well so the outer tip passes between the outer edge of
the insert and the inner well of the insert. Release the electrode so that it stands free,
then after a few seconds record the reading. Slide the cable on the table toward the
plate and electrode to prevent tipping.
Theory of Operation
The EVOM3 system uses a four electrode sensor, two for current sourcing and two for
voltage measurement.
The EVOM3 passes a known constant current through the membrane on two
electrodes and measures the voltage needed to pass that current on the other two
electrodes and computes resistance using Ohms law. The polarity of the current
changes from positive current to negative current 12.5 times per second. This polarity
change avoids leaving a charge on the membrane and negates any voltage osets due
to the membrane potential or from the electrodes. The amount of current and voltage
is low (2, 4 or 10µA) to minimize any tissue stimulation and to prevent the migration
of metal ions.
Main Display
Touch to short electrodes
Preview the plate by its
layout
File name, defined in the
storage options menu
Current cell in plate Battery indicator
Range (V, mV, Ω, KΩ)
Press to record and index
to the next well. Press and
hold to open the storage
options menu
Fig. 6—The main EVOM3 display.
A negative resistance number will appear in red test. This can be caused by a faulty
electrode in media, (see "Troubleshooting" on page 31) or a blank setting that is
over the measurement (see "Blank Handling Page" on page 13).

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Setup Page
Press Setup on the main screen to open the Setup page.
Fig. 7—Setup page
Select Plate – Select the number of wells and the sampling pattern.
Mode Units – Choose the Units for sampling. (Options include Millivolts, Volts, Ohms
and K Ohms.)
Blank Handling – Lets you subtract the control blank from the current resistance
measurement.
Short Electrodes – Shorts V1-V2. and is used to nullify the galvanic charge in the
electrodes when the electrodes are placed in a saline solution for 4–8 hours to
remove the charge. See also "Probe Null" on page 14.
Date/Time – Select the Date and Time formats for your data les.
Calibrate – Calibrate the electrode. (This uses a 10K Ω 0.1% resistor.) See "Instrument
Diagnostics and Calibration" on page 21.
Probe Null – Neutralize the oset charge on V1-V2..
Store Screen– Store data les, congure how the les are named and if they are
automatically numbered.
Plate Selection Page and Plate Preview
1. Press Select Plate on the Setup page to access the Plate Selection screen.
Fig. 8—Choose the number of wells and the sampling pattern.
2. Select the radio button for the number of wells in your plate.
3. Select the sampling pattern from the options in the bottom half of the window.

12 World Precision Instruments
4. Press
Return to save the setup. When you press Preview from the main screen
you will see the plate preview les like the samples below.
Fig. 9—(Left) 6-well plate preview
Fig. 10—(Right) 12-well plate preview
Fig. 11—(Left) 24-well plate preview
Fig. 12—(Right) 48-well plate preview
Fig. 13—96-well plate preview. (Press scroll to see the additional wells.)
NOTE: An out of resistance range will display ---- (5 dashes) on the screen. Adjust the
range to correct the error. A saved well under preview will show a space (“ “) if the
display was recorded out of range. The 100K range can read 5% over the 105K Ω.
Mode Units
1. Press Mode Units on the Setup page to access the Select Volts or Ohms screen.
Fig. 14—Choose the unit of measurement and (if you are using Ohms) the range.

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2. Choose your units. Options include Millivolts, Volts, Ohms and K Ohms. If you
choose ohms, then you must also select an Ohms Range.
Fixed Current Variable Current
10 K 10 µA Auto 2 µA to 10 µA
50 K 4 µA
100 K 2 µA
3. Press
Return .
Blank Handling Page
1. Press Blank Handling on the Setup page to subtract the control well blank from the
current resistance measurement.
Fig. 15—(Left) Blank Handling window.
Fig. 16—(Right) The main screen shows the oset underneath the reading.
2. Insert the probe into an empty well lled with media. Press
Reading to make a
measurement.
3. Select the
Blank Enable radio button to enable the oset.
4. Press
Return . The resistance oset shows under the reading on the main screen.
If the blank (oset) is greater than the resistance reading, the reading will display
negative numbers in red text. The saved data le reects the subtraction.
NOTE: This includes uid and electrode resistances.
Setting the Date and Time
1. Press Time/Date on the Setup page to set the date and time format.
Fig. 17—The Set Date/Time screen lets you set the date and time and choose the display format.
2. Press Set Date . Set the appropriate date and press Return .

14 World Precision Instruments
3. Press
Date Format . Choose your preferred format and press Return .
Fig. 18—(Left) Set the date and press Return
Fig. 19—(Right) Choose the date format and press Return
4. Press
Set Time . Set the appropriate time and press Return .
5. Press
Time Format . Choose 12 hour or 24 hour and press Return .
Fig. 20—(Left) Set the time and press Return
Fig. 21—(Right) Choose the time format and press Return
Probe Null
1. Press Probe Null on the Setup page to access the NULL Probe Process screen.
Fig. 22—(Left) Null probe process.
Fig. 23—(Right) The potential dierence oset shows on the main screen under the reading.
2. Place the electrodes in a saline media.
3. Push the
NULL button. Wait for the second message stating ”Solution value 0.0”
before returning. This action removes any voltage osets on the electrodes and
neutralize the oset charge on V1-V2.
4. Select the Enable/Disable radio button to use the oset.
5. Press
Return . The potential dierence oset shows on the main screen under the
reading. An electrode check should show 0.0 mV. The saved data le reects the
oset.

EVOM3
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Storing Data
1. Press and hold the Store button on the main screen for 2 seconds or press Store
Screen on the Setting screen to congure your stored data les.
Fig. 24—Use the Store Options screen to save a data le.
2. Press
New Plate to clear any recorded data on the preview screen and from
memory.
CAUTION: This will clear any readings saved in the preview grid.
3. Press
File Name to enter a unique name for your data le. In the entry page, select
Clear , then type the le name, and press Enter when you nish. If you choose to
use your le name, auto-indexing must be disabled.
Fig. 25—Press Clear. Type the desire name. Press Enter.
4. If you prefer to use the
Auto Index function, press Store Prex to enter a unique
le name that will be appended with sequential numbers each time your le is
saved. Select the Auto Index radio button to enable the Store Prex and Auto Index
buttons .
5. Press Auto Index if you want the EVOM3 to automatically number the new data
les sequentially. Auto indexing changes the name to a numeric sequence
formatted with a prex and a sequential number (prexN).
6. Press Store Now to save the current data set. There is a time out if the USB write
fails. If this error occurs, the “Saving le…” notice will not change to “File saved.”
A le is typically saved in less than 20 seconds. If your le does not save, press
Return and try using a dierent USB drive.
7. Press
Return .

16 World Precision Instruments
Viewing Data in Microsoft Excel or Other Spreadsheet
Program
1. Remove the USB thumb drive from the EVOM3 and plug it into a free USB slot in
your computer.
2. Open the drive and look for les named
PlateX.CSV .
3. If your computer has Microsoft® Excel or another spreadsheet program the le
will open by clicking on the le. The well data is loaded into spreadsheet cells.
4. To transfer the data le to your computer, select the plate les and copy it to a
folder on your computer.
Sample resistance le shows:
• Date, time, plate size
• EVOM3 rmware revision
• Measurement mode with a blank or a voltage oset
• Units ohms or kilo ohms
• Recording direction, 1-4 (See "Plate Selection Page and Plate Preview" on
page 11.)
• Resistance scale (10K, 50K, 100K), and the last used scale is shown
• Well data in the plate grid with the blank oset applied.
Sample Resistance File (*.CSV)
Date 03/12/2020 13:11:56 Well Plate 24
EVOM3 Version 1.0
MODE OHMS oset 999.8
Units: OHMS
Direction 1
Resistance Scale 50k Ohms
0123456
A 19015 19015 19015 19015 19015 19015
B 19016 19016 19016 19016 19016 19016
C 19016 19016 19016 19016 19016 19014
D 19014 19014 19014 19014 19014 19017
Sample Voltage File(*.csv)
Date 03/12/2020 13:12:48 Well Plate 24
EVOM3 Version 1.0
MODE PD oset -0.0 mV
Units: mV
Direction 1
Resistance Scale 50k Ohms
0123456
A 0.0 0.0 0.0 0.0 0.0 0.0
B 0.0 0.0 0.0 0.0 0.0 0.0
C 0.0 0.0 0.0 0.0 0.0 0.0
D 0.0 0.0 0.0 0.0 0.0 -0.0