3D Histech PANNORAMIC MIDI RX 1.0 User manual

PANNORAMIC® CONFOCAL RX 1.0
USER’S GUIDE
May 13, 2021 –Rev.1
FOR RESEARCH USE ONLY. NOT FORUSE IN DIAGNOSTIC PROCEDURES.

PANNORAMIC® CONFOCAL RX 1.0
TABLE OF CONTENTS
MAY 13, 2021
1
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Table of Contents
Table of Contents ................................................................................................................. 1
Disclaimer............................................................................................................................4
Declaration of Conformity......................................................................................................6
About this User's Guide.........................................................................................................7
Character Formats and Symbols.............................................................................................8
Notes Regarding Operational Safety.......................................................................................9
Notes on Warranty...............................................................................................................13
Terms and Abbreviations..................................................................................................... 14
1Product Description......................................................................................................15
1.1 Product overview 15
1.1.1 Intended use......................................................................................................15
1.1.2 Features and benefits ........................................................................................ 16
1.1.3 Warning and information labels ...........................................................................17
1.1.4 Base unit............................................................................................................17
1.1.5 Connector panel................................................................................................ 18
1.2 System overview 19
1.2.1 Pannoramic® Confocal RX System....................................................................... 19
1.2.2 Hardware options..............................................................................................20
1.2.3 Control software ................................................................................................21
2Installation.................................................................................................................. 22
2.1 Operation system of the control computer 22
2.2 Control computer ports 24
2.3 Connectors and cables 24
2.4 Power the scanner on/off 25
3Operating Slides and Trays............................................................................................26
3.1 Preparing slides and trays 26
3.2 Affixing barcode stickers to slides 27
3.3 Inserting and removing slides 28
4Software Description ....................................................................................................31
4.1 Starting / exiting Pannoramic® Scanner Software for research control software 31
4.1.1 Starting Pannoramic® Scanner Software for research control software ...................31
4.1.2 Exiting Pannoramic® Scanner Software for Research control software....................31

PANNORAMIC® CONFOCAL RX 1.0
TABLE OF CONTENTS
MAY 13, 2021
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
4.2 Main window functions 32
4.3 Hotkeys 51
5Scanning Modes and Settings ....................................................................................... 53
5.1 Brightfield Scanning Mode 53
5.1.1 Profile settings panel .........................................................................................54
5.1.2 Microscope controlpanel................................................................................... 61
5.1.3 Quick preview functions..................................................................................... 72
5.1.4 Quick microscope functions................................................................................ 72
5.2 Fluorescence Microscope Mode 74
5.2.1 Profile Settings Panel.........................................................................................74
5.2.2 Microscope Control Panel...................................................................................85
5.2.3 Quick preview functions.....................................................................................97
5.2.4 Quick microscope functions................................................................................97
5.2.5 Bottom menu bar of the main window .............................................................. 100
5.3 Planner mode 101
5.3.1 General functions at the main menu ................................................................... 101
5.3.2 General functions at the tray menu.....................................................................103
5.3.3 General functions at the slide menu................................................................... 104
5.3.4 Slide view with card.......................................................................................105
5.3.5 Table view mode ............................................................................................. 108
5.3.6 Card collection view mode............................................................................ 109
5.4 Profile and preview overview on the planner 109
5.5 Progress features 110
6Settings and Services ...................................................................................................111
6.1 Settings 111
6.2 Services 120
7Point Spread Function.................................................................................................130
8Using Profiles.............................................................................................................132
9Troubleshooting and Maintenance ...............................................................................134
9.1 Activation of the Control Software 134
9.2 Recovery functions 139
9.3 Safety information 139
9.3.1 Cleaning the device.......................................................................................... 140
9.3.2 Cleaning the objectives................................................................................. 140

PANNORAMIC® CONFOCAL RX 1.0
TABLE OF CONTENTS
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
9.3.3 Cleaning the trays ........................................................................................ 140
9.3.4 Preventive maintenance ................................................................................ 141
9.4 Troubleshooting 141
9.4.1 Fluorescent applications....................................................................................143
9.5 Maintenance 144
9.5.1 Opening the service door ................................................................................. 144
9.5.2 Replacing fuses of the main power switch .......................................................145
9.5.3 Removing and refilling the pipette................................................................. 146
9.5.4 Removing a slide.......................................................................................... 149
9.5.5 Installing and removing filter cubes ................................................................150
9.5.6 Transporting.................................................................................................153
9.5.7 Removing and cleaning the condenser............................................................154
10 Technical Data............................................................................................................157

PANNORAMIC® CONFOCAL RX 1.0
DISCLAIMER
MAY 13, 2021
4
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Disclaimer
Copyright © 2001-20213DHISTECH Ltd.
All rights reserved.
Intellectual property related to the 3DHISTECH products are as follows:
•P1300124;PCT/HU2014/000020; EP 14745228.8.;US 14/771.380
•P0700404;PCT/HU2008/000065;EP 08762668.5.;US 12/663.537
•P1700279; PCT/HU2018/050030
•P1700483
•P1500394;PCT/HU2016/050039; EP 16840280.8.; US 15/756.370
•P0700409; US2008/0306964
•P0800181;PCT/HU2009/000027; EP 09721248.4.
•P0600435;PCT/HU2007/000047;EP 2024772; US12/301.781
•P0600177;PCT/HU2007/000018; EP 1.994.398;US12/281.566
•P0900741; PCT/HU2010/000130;EP 10812989.1.
•P0900142; PCT/HU2010/000027;EP 10726183.6
•P101036; PCT/IB2005/050351;EP 1789831; US 7.663.078
•P0301911; PCT/HU04/00019;EP 1644767; US 7327515
The above-mentioned grantedpatents,grantedutility model, registered trademarks, pending
internationaland national patent applications and the othercorresponding IP are the sole
properties of the 3DHISTECH Ltd.
3DHISTECH Ltd. is not liable for damage of whatever nature (including, but not limited to,
general or specificdamage, indirect damage, consequentialdamage orincidentaldamage,
includingthe results of the analysis of the digitizedslides, for example, change of health status
related to erroneous diagnosis from the digitized slide(s)) that stems from or is associated with
use of Product, digitized slides, quality of staining,quality of stainedslides, qualityof used
method of staining.3DHISTECHLtd. assumesno responsibility forthe functionalityand fault-
free condition of your “application programs” (Workflows, VBA macros, Commander scripts).

PANNORAMIC® CONFOCAL RX 1.0
DISCLAIMER
MAY 13, 2021
5
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
CAUTION
For Research Use Only. Not for use in diagnosticprocedures. This product has not been approved
or cleared as a medical device by theU.S. Food and Drug Administration,nor any otherregulatory
authority. Thedata and images obtainedor viewed using this product are not intended for clinical
or diagnosticuse.
For performanceevaluationonly, the performance characteristics of this product have not been
established.
Third-party disclaimer
3DHISTECH Ltd. is not liable for damage of whatever nature (including,but not limited to, general
or specificdamage, indirect damage, consequentialdamageor incidentaldamage,including
damage resulting fromlost profits, interruptionof business,loss of business information,
pecuniary loss or similar damage) that stems fromor is associated with the incorporated part of
this Product that is made by a third-party company.
THE SOFTWARE IS PROVIDED “AS IS” AND WITHOUT WARRANTY OF ANY KIND, EXPRESSED,
IMPLIEDOR OTHERWISE, INCLUDING WITHOUTLIMITATION, ANY WARRANTY OF
MERCHANTABILITY OR FITNESS FOR A PARTICULARPURPOSE.
FurtherInformation
For the latest information on 3DHISTECH products and services, please visit our website at the
following URL: http://www.3dhistech.com.
Company Address
3 Öv Street, 1141 Budapest –HUNGARY

PANNORAMIC® CONFOCAL RX 1.0
DECLARATION OF CONFORMITY
MAY 13, 2021
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Declaration of Conformity
3DHISTECH Ltd. declares that the product Pannoramic® ConfocalRX is designedand produced
with consideration of the quality management system certified accordingto the ISO 13485:2016
standard, and conformsto standardslisted below:
IEC 61010-1:2010 (ed. 3), “Safety requirements for electrical equipment for measurement, control,
and laboratory use –Part 1: General requirements”
EN 61326-1:2013 “Electrical equipment for measurement, control and laboratory use –EMC
requirements –Part 1: General requirements”
EN 61010-1:2010 Safety requirements for electrical equipment for measurement,control, and
laboratory use –Part 1: General requirements
EN 61326-1:2013
EN 55011:2009+A1:2010
EN 61000-3-2:2006+A1:2009+A2:2009
EN 61000-3-3:2013
Further informationmaybe obtained from the manufacturer:
3DHISTECH Ltd.
3 Öv Street, 1141 Budapest –HUNGARY

PANNORAMIC® CONFOCAL RX 1.0
ABOUT THIS USER'S GUIDE
MAY 13, 2021
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
About this User's Guide
The purposeof this manual is to assist the user in the safe and reliable operation of the product
described. Before operating thedevice, you must carefully read this instructionmanual, and
adhereto all WARNING and CAUTION notices (refer to chapter Notes RegardingOperational
Safety for more information), anda copy should be kept in the close proximity or near the
scanner for reference whenever required.
This User's Guide is a part of the Pannoramic® ConfocalRX systemidentified by the relevant
article numbers included in the OrderingGuide.

PANNORAMIC® CONFOCAL RX 1.0
CHARACTER FORMATS AND SYMBOLS
MAY 13, 2021
8
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Character Formats and Symbols
The following character formats and symbols call your attentionin this manual:
Example (text in bold)
Words or characters that appear on thescreen. These include field names, screen and window
titles, push-button and menu names, paths or options.Keys on the keyboard. For example,
function keys (such as F11) or the Ctrl+Shotkey.
Example (text in bold and italics)
Cross-references withinthis document or to other documents.
Warning!
It might cause irreversible damage or harmto the user of the product or the product itself if
this instruction is not followed.
Caution!
It might cause the product to not work properly if this instruction is not followed and alerts
to a potential danger situation.
Notice:
Calls attention to some important features.

PANNORAMIC® CONFOCAL RX 1.0
NOTES REGARDING OPERATIONAL SAFETY
MAY 13, 2021
9
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Notes Regarding Operational Safety
The below section of this User’s Guide contains information andwarnings of a kind that must be
followed by owner/operatorpersonnel. Warning and advisorysymbolswhichareused on the
base unit of the Pannoramic® Confocal RX and in section 1.1.3 Warningand Information Labels
havethefollowing meanings:
Disconnect mains plug!
Remember to unplugthe power cord before you open the Pannoramic® Confocal RX main
unit for maintenance.
Crush hazard!
There is crush hazard at the following areas of the device:
•At the movement area of the slide tray during operation. Please note that the
digitizing process has to be interrupted and the tray moved via the software to the
output position before removing it.
•The tray feeder door, the cross-beltarea of the tray guide, and thesafety plate
during operation.Please notethat the digitizing process has to be interrupted and
the tray moved via software to the output position before removal.
•The two hinges of the service door.
•The inner side of the door opener mechanism.
•When reaching by the pipette unit into the farthest area of the device.
The Pannoramic® Confocal RX, includingany of its original accessory components,shouldnot be
operated in any other way than described in chapter 1.1.1 Intended use.Any of thefollowingnotes
should bestrictly observed:
Warning!
The Pannoramic® Confocal RX may notbeoperated by other than properly instructed
persons. Operatingpersonnel must be fully aware of the potential dangers which a
particular field of application involves. The Pannoramic® Confocal RX is a precision
instrument which maysuffera significant reduction in operational functions or even physical
destruction following interventionperformed in any nonconforming manner. If the
equipment is used in a manner not specified by the manufacturer,the protection provided
by the equipmentmay be impaired.

PANNORAMIC® CONFOCAL RX 1.0
NOTES REGARDING OPERATIONAL SAFETY
MAY 13, 2021
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Warning!
If operating the deviceother than specified by the manufacturer,the levelof safety will
decrease.
Warning!
Insert the power cord plugs only into a grounding outlet. The protective action must not be
rendered ineffective by extension cables without protective conductor.The Pannoramic®
Confocal RXhas a built-in protection which mustnotberendered ineffective by extension
cables without protective (earth-ground) conductor.
Warning!
Never operate the device in explosion-risk environment.
Warning!
If protective devices are found to be ineffective, the Pannoramic® Confocal RX must be shut
down and precautions must be taken to prevent unintended usage.For necessary repair
work or work to restore proper operating condition, youshouldcontactthe 3DHISTECH
Customer Service.
Warning!
Check that your local line voltage is compatible with Pannoramic® Confocal RX
specifications before you turn power on.
Warning!
Only connect UL-approved SELV (safety extralow voltage)rated externaldevices to the
instrument to avoid the risk of electrical shock.

PANNORAMIC® CONFOCAL RX 1.0
NOTES REGARDING OPERATIONAL SAFETY
MAY 13, 2021
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Warning!
The Pannoramic® ConfocalRX does not include any specialfacilities to protect from samples
with an etching, potentially infectious, toxic, radioactive, or otherhealth-damagingeffect.
You are under obligation to comply with allcurrently binding legislation, notably,national
accident prevention rules when handling samples of this kind.
Warning!
Use of this instrument in a dry environment, especially if syntheticmaterial is present
(syntheticclothing, carpets,etc.) may cause damagingelectrostatic dischargesthat may
cause erroneous results.
Warning!
A defective Pannoramic® Confocal RX is not classified as domesticwaste. It must be
properly disposed in accordancewith currently valid legalrequirements.
Caution!
In the event of any non-conformingtypeof use, including non-conforming use of assemblies
or single parts, no liability willbe assumed by the Manufacturer. This shallalso apply to
service or repair work of any kind, which is performed by other than authorized service
personnel. Allclaims for warranty and warrantyservices willbenulland void in any such
case.
Caution!
Dirt or dust may adversely impact the operating capability of the Pannoramic® Confocal RX.
For this reason, measures must be taken to eliminatesuch influences on a maximum
possibledegree. During periods of non-use, the Pannoramic® ConfocalRX mustbe
protected with a dust cover. Check that power is turned off before you put the cover on .

PANNORAMIC® CONFOCAL RX 1.0
NOTES REGARDING OPERATIONAL SAFETY
MAY 13, 2021
12
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Caution!
Do not position the equipmentso that it is difficult to operate and to disconnect the device
if necessary.
Notice:
The safety level of the equipment afterservicing willbe identical with levelof theoriginal
equipment.
Notice:
Electric power to the Pannoramic® ConfocalRX is supplied via an external mains power
supply unit. It allows for operation at line voltages in the range of 100 V to 240 V ±10%, 50/60
Hz, without any extra facilities required for voltage transformation.
There is no further operator (user) action required in case of residual risks.
Special attention should bepaid to allinformation provided and the procedures described in
section 8.3 Safety information.

PANNORAMIC® CONFOCAL RX 1.0
NOTES ON WARRANTY
MAY 13, 2021
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Notes on Warranty
3DHISTECH Ltd. –as the Product Manufacturer –warrantsthe Pannoramic® ConfocalRX to be
free from faults in material and workmanship at the moment of installation. Defects must be
notified immediately on identificationand maximumefforts must beundertaken in order to
minimizedamage. Onreceiving noticeof a fault, the Product Manufacturer willbe under
obligation to remove the fault, at his own option, either by providingadequate repair services or
by delivering a faultless product for replacement. No warrantywill be accepted in cases of natural
wear (notably, wear and tear) or improper product handling.
The Product Manufacturer will refuse any liability in the event of damage resulting from faulty
operation, negligenceor unauthorized interventioninto the product, notably,from the
replacement of product parts or the usage of accessory parts from other manufacturers.Any
such case will void the user’s claim for warranty.
Except for actions which are explicitly described in this User’s Guide, you are prohibited from
performing anykind of maintenanceor repair work at the Pannoramic® Confocal RX. Only
authorized 3DHISTECH Servicepersonnelor specifically authorized representatives areallowed to
handlerepair work. In case of malfunction contact3DHISTECH ServiceSupport first.

PANNORAMIC® CONFOCAL RX 1.0
TERMS AND ABBREVIATIONS
MAY 13, 2021
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Terms and Abbreviations
Term
Description
CSV
Comma Separated Value
FOV
Field of View
NA
Numerical Aperture
Photobleaching
A phenomenon that leads to a dramatic loss of fluorescence emission intensity (a
fluorophore permanently loses the ability to fluoresce) due to photon-induced chemical
damage and covalent modification, therefore fluorescent signals cannot be properly
detected.
SWAP
A file that is used as a temporary location on the computer to store information that is not
currently used by the RAM.
Virtual Slide / Slide
A digital image of a thin glass plate on which specimens are mounted for microscopic study:
a dynamic, interactive image that you can manage, save, magnify, zoom, name, evaluate,
annotate, mark, comment, send it to a colleague electronically for co-operation or advice,
and so on.
Table 1: Terms and abbreviations

PANNORAMIC® CONFOCAL RX 1.0
PRODUCT DESCRIPTION
MAY 13, 2021
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
1Product Description
1.1 Product overview
1.1.1 Intended use
The Pannoramic® Confocal RX is a digital scanner microscope installed with an Aurox CC88
confocalunit and high-numericalaperture immersion and/or dry objectives designedto use
transmitted light and reflected light fluorescenceto digitize biologicalsamples and specimens
(histological sections, typically) based on confocalprinciples.
Warning!
The Pannoramic® ConfocalRX devicemust beoperated by a properly instructed person
only. Operating personnelmust be fully aware of the potential dangers whicha particular
field of application involves. ThePannoramic® ConfocalRX is a precision instrumentwhich
may suffer a significant reductionin operational functions or even physicaldestruction
following interventionperformed in any nonconformingmanner.
Warning!
After each scanning make sure to inspect the digital slide for over-exposed areas. In case the
slide is scanned based on improperly set parameters,it can highly affect the result because
of information loss.
Warning!
Any modificationto a hardware or software component of the PC will highly affect the
functionality of the scanner program.
Warning!
Do NOT install any other application than the Pannoramic® Scanner Softwarefor Research
control software application and itsauxiliary drivers on thecontrol computer.

PANNORAMIC® CONFOCAL RX 1.0
PRODUCT DESCRIPTION
MAY 13, 2021
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
1.1.2 Features and benefits
The Pannoramic® Confocal RX offers world-class brightfield andfluorescent whole slide scanning.
An outstanding0.16 µm/pixel(bothin Brightfield and Fluorescent modes) resolutionis achieved
with the 40×/1.2 NA (equivalent to 62× magnification) C-Apochromat objective.
Key features of the Pannoramic® Confocal RX scanner:
•Outstandingimage resolution achievedby usingAuroxCC88 confocalunit
•Widefield Fluorescent, Confocal Fluorescent, andBrightfield imaging
•Darkfield preview for low intensity samples
•Pre-Scan function for FL preview imaging
•Up to 4 filter cubes (quad or dual) for fluorescence
NOTE: The number of channels available depends on the typeof the applied fluorescent
illumination device.
•High-end fluorescent illumination device –Lumencor SPECTRA Light Engine
•Multiband filter support
•FL scanning with a low-noise, 16-bitscientific CMOS pco.edge 5.5 camera
•Software-aimed flat-field correction compensation method to create homogeneous slide
image
•12-slide automaticloadingand scanning
•Motorized objective changer
•Automated immersionfor high NA objectives
•Improved permanent autofocus
•Compact and robust device
•Good quality and stablemanufacturing
•High-resolutionpreview camera for 1D and 2D barcode reading
•Image sharpening(for both Fluorescenceand Brightfield) feature to improve the visual
appearanceof digitalslides
•Focus algorithm finds the focus whichis most appealingto the human eye

PANNORAMIC® CONFOCAL RX 1.0
PRODUCT DESCRIPTION
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
1.1.3 Warning and informationlabels
The following figure contains the labels used on the back panel, and on the inside of Pannoramic®
ConfocalRX main unit.
Figure1: Warning and information labels
1.1.4 Base unit
The following figure shows the main outerparts of the device.
Figure 2: Main parts of the base unit

PANNORAMIC® CONFOCAL RX 1.0
PRODUCT DESCRIPTION
MAY 13, 2021
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
1) Service door
2) Door securing lock
3) Slide tray feeder door
4) Cable outlet
5) Connector panel (USB port, power supply connector, fusebox)
6) Transport handle (4xatthe four corners of the device)
7) On/Off button
1.1.5 Connector panel
The following figure details the sub-assemblies of the connector panel.
Figure 3: Sub-assembliesofthe connectorpanel
1) USBport for controller PC
2) Fusebox
3) Main power switch
4) Main power inlet

PANNORAMIC® CONFOCAL RX 1.0
PRODUCT DESCRIPTION
MAY 13, 2021
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
1.2 System overview
The standard scope of delivery of the product includes the following hardwareand software
components.
1.2.1 Pannoramic® Confocal RX System
The following figure shows the main partsof the Pannoramic® Confocal RX system.
Figure 4: Pannoramic®Confocal RX system overview
1) Pannoramic® ConfocalRX base unit
2) Slide tray