AccuStrata FLUOROTEK 200 User manual

Revision 1.0 August 1, 2016
www.accustrata.com
FLUOROTEK 200
Handheld Fluorometer
Instruction Manual
AccuStrata, Inc.
Parklawn Business Park
11900 Parklawn Dr., Suite 203
Rockville MD 20852
[phone]
ATTENTION: You Must Read This Manual before Using

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AccuStrata Fluorotek 200 User’s Manual
Contents
1. Introduction .......................................................................................................................................... 2
1.1. Description.................................................................................................................................... 2
1.2. Quick View Diagram...................................................................................................................... 2
1.3. Inspection and Setup .................................................................................................................... 2
1.3.1. Inspection.............................................................................................................................. 2
1.3.2. Setup ..................................................................................................................................... 2
1.4. General Information, Precautions and Cleaning........................................................................... 3
2. Instrument Operation ........................................................................................................................... 3
2.1. Instrument Power Up.................................................................................................................... 3
3. Sample Analysis Guidelines................................................................................................................... 4
3.1. Handling Samples.......................................................................................................................... 4
3.2. Positioning Samples ...................................................................................................................... 4
3.3. General Data Acquisition Instructions .......................................................................................... 5
3.4. Reviewing Data ............................................................................................................................. 7
3.4.1. Review Data on Fluorotek 200.............................................................................................. 7
3.4.2. Review Data with PC Software.............................................................................................. 7
4. Software................................................................................................................................................ 7
4.1. System Requirements ............................................................................................................... 7
4.2. Software Installation................................................................................................................. 7
4.3. Troubleshooting......................................................................................................................10
5. Applications.........................................................................................................................................11
5.1. EnZtek Product Line ....................................................................................................................11
6. Specifications ......................................................................................................................................12
7. Warranty .............................................................................................................................................13
7.1. Terms ..........................................................................................................................................13

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AccuStrata Fluorotek 200 User’s Manual
1. Introduction
1.1. Description
The Fluorotek 200 is a small form factor handheld fluorometer that is optimally designed for use
in the detection of bacteria. Dual channel design allows users to test for a variety of bacteria
using various assays.
1.2. Quick View Diagram
1.3. Inspection and Setup
1.3.1. Inspection
Upon receiving your device, please inspect everything carefully and make sure all accessories
are present. All devices include:
The Fluorotek 200 handheld unit.
Light-blocking cap for sample holder
A USB drive containing PC software and pdf documents for quick start and user’s
manual.
1.3.2. Setup
Battery installation:
1. On the back side of the instrument, remove the battery panel by pressing slightly on the
corrugated tab with thumb and pulling down.

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2. Install 4 - AA batteries as indicated at the bottom of the battery housing.
3. Replace the battery protection panel by inserting the panel into the groves and sliding up
into position.
1.4. General Information, Precautions and Cleaning
The sample compartment is designed to accommodate standard polypropylene 0.5 ml
Polymerase Chain Reaction tubes as shown below:
Any other sample holder besides those displayed above are not guaranteed to fit
properly in the sample holder. Do not use solvents inthe test tube that may penetrate or
degrade the integrity of the tube. See tube manufacture instructions for further
information.
If a sample is accidentally spilled inside the sample compartment, invert the Fluorotek
200 unit to drain the fluid from the compartment. Then carefully wipe the inside of the
compartment with a clean cotton swab or cloth. Allow the unit to dry before using.
The Fluorotek 200 is NOT waterproof and should be kept away from water and moisture.
Do not expose the Fluorotek 200 unit to temperatures below 5 ⁰C or above 40 ⁰C.
2. Instrument Operation
2.1. Instrument Power Up
To turn on the Fluorotek 200, toggle the power switch on the top right side of the unit to the left.
To turn the unit off, toggle the power switch right.
If the Fluorotek 200 is left on for more than 30 minutes, the unit will power off automatically.
Once the system is turned on, a LOADING SCREEN will appear followed by the HOME SCREEN:
Once the HOME SCREEN appears, the system is ready for use.

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3. Sample Analysis Guidelines
3.1. Handling Samples
Keep the sample chamber dry. Do notspill liquid into the sample chamber.
If a spill occurs, immediately invert the unit to remove the liquid.
Allow to fully dry before proceeding.
Condensation on the outside of test tube may result in read errors. Ensure that the
sample temperature has reached ambient temperature before placing the tube into the
sample chamber.
Never reuse test tubes for multiple samples
Bubbles or aeration within the sample may result in read errors. To remove air bubbles
from the sample, hold vertically and rapidly tap the outside of the tube with fingers.
3.2. Positioning Samples
The orientation of the test tube may result in slightly different readings, especially for low
fluorescence readings. This is due to inconsistency in quality of the polymer test tube. For
proper use, ensure that the tab of the tube is oriented the same way for every measurement.

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3.3. General Data Acquisition Instructions
To take a measurement press the Measure button on the HOME SCREEN. This will load the
CHANNEL SCREEN. Select the desired channel to use for the measurement to load the
MEASUREMENT SCREEN.
To ensure that thetest tube does not influence the result, the tube and media must be zeroed.
To do this, insert the blank test tube (tube with media absent of fluorescing material) and press
Blank. This will bring up the TESTING SCREEN.
The value measured through this process will be subtracted from any future measurement
results. To reset the blank value to zero, use the Return button to load the HOME SCREEN.
Once the sample has been blanked, a measurement can be taken using the Measurement
button. This button will initialize the TESTING SCREEN followed by the RESULTS SCREEN.

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The top of the TEST SCREEN displays the number of minutes at which the measurement was
taken after the test was initiated. Once the measurement is complete, the RESULTS SCREEN is
displayed. The RESULTS SCREEN displays the time of the reading and the measured values,
which is:
𝑅𝑒𝑎𝑑𝑉𝑎𝑙𝑢𝑒 = 𝑅𝑎𝑤𝑅𝑒𝑎𝑑 − 𝐵𝑙𝑎𝑛𝑘𝑅𝑒𝑎𝑑
After the first read, a 10 minute timer is started. This is the average amount of aging required for
specific bacteria detection reagents to sufficiently fluoresce. Once the 10 minute time is
reached, a second measurement is automatically taken. While the timer is counting, the user
can save the first read to memory using the Save button or cancel the read by using the Return
button. To the right of the timer is the data save location. Each light channel has a set of
memory slots that are numbered. When a new reading is saved to the system, the value is
assigned to a memory slot that has an integer value associated with it. This integer value that
represents the data slot that the measured value will be stored in is displayed to the right of the
timer value.This number also corresponds to the table position of downloaded values on the
software application. The values can also be reviewed on the system itself.

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3.4. Reviewing Data
3.4.1. Review Data on Fluorotek 200
To review data that has been saved to the Fluorotek 200, press the Data button on the HOME
SCREEN. This will display the CHANNEL SCREEN. Select the wavelength that the data to be
reviewed was taken with. Finally, the DATA SCREEN will be displayed. The DATA SCREEN allows
the user to scroll through all of the datasets currently saved on the unit by pressing the <- and -
> position on the <-0000-> button.
3.4.2. Review Data with PC Software
First attach the Fluorotek 200 unit to the computer. Next, open the software application. Finally,
press the Read All Data button. The data will be displayed in tabulated form on the right of the
application screen. If the Fluorotek 200 unit is connected to the computer after the application
has started, First, press the Refresh COM List button followed by the Read ALL Data button.
See the software section of this manual for more details about the application.
4. Software
Users of the Fluorotek 200 handle held fluorometer are provided with a companion computer
application that can be used to read test results and transfer them to a computer for further
recordkeeping and analysis. The application is not required to use the Fluorotek 200.
4.1.System Requirements
Fluorotek 200 Software
Operating System
Windows® 7, 8, 8.1, 10
OS Bit
64 bit
Connection
USB 2.0
Please ensure that the 64-bit java JRE is installed on the PC.
4.2.Software Installation
Download the Fluorotek 200 Software Installer
ohttp://www.accustrata.com
Double click on the Fluorotek 200 installer

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Accept the installation
Press the Next> button
Choose the file system location to install the software
Choose the location on the Start Menu to place a shortcut to the software

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Review your installation selections and press Install
A setup menu will appear asking if you would like to install the USB Drivers for the Fluorotek 200.
These drivers are required to successfully communicate with the Fluorotek 200. Select Yes to
proceed.
The driver installation menu will appear. Press Extract to unpack the driver files.
The driver installation window will appear. Select Next>.

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Read and accept the agreement terms by selecting the I accept this agreement radio button and
press Next.
You will receive a confirmation screen that says the driver packages have been installed.
An information screen will appear indicating the drivers have been successfully installed.
A final screen will appear saying that the installation has completed.
4.3.Troubleshooting
Q: Why won’t the software install in the beginning steps?
A: Some virus scanning software will block the installation of the software. Try temporarily
disabling your virus protection software.
Q: After double clicking the installer, a message appears saying that I need elevated
privileges. Why?
Some versions of windows require elevated privileges to install. To install with elevated
privileges, right click on the installer and select Run as Administrator:

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4.4.Software Overview
Port Selection- This button allows the user to select the communication port to which the
Fluorotek 200 unit is connected. There is also an AUTODETECT option which will have the
application automatically search for the appropriate communication port.
Port Refresh- This button updates the available communication ports. This can be used to
rediscover the Fluorotek 200 unit in the event that it is connected to the computer after the
application is launched.
Connection Test –This button will run the connection protocol to detect the Fluorotek 200 unit,
but will not download the data.
Data Read –This button will run the connection protocol and, if the Fluorotek 200 unit is
detected, will download and display the data in the Data Display section.
Erase Data –This button will clear the data from the Fluorotek 200 unit.
Save Data to File –This button will allow the user to save all of the data that is on the Fluorotek
200 to a file that can be imported into any spreadsheet software.
Exit Application –This button will shut down the application.
5. Applications
5.1. EnZtek Product Line
The Fluorotek 200 is optimized for use with EnZtek brand bacteria testing reagents. Please
consult the chart below in Specifications in order to determine the correct wavelengthat which to
test your sample.
NOTE: Threshold values are specific to each reagent. The Threshold values will be supplied
with the individual kits.

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6. Specifications
The Fluorotek 200 device specifications are:
Fluorotek 200
Size
7.1" x 3.8" x 1.3"
Weight
~ 1 lb
Resolution
12 bits
Display
160x104 pixels
Temperature
41⁰F -104⁰F | 5⁰C -40⁰C
Storage Temperature
Source
360 nm & 490 nm LED
Detector
Photodiode
Power
4-AA Batteries
Calibration Type
None
Error Message
None
Supported Sample Vial
0.5 ml Polymerase Chain Reaction tubes
Warm Up Time
3 seconds
Auto Shutdown Time
30 minutes
The software specifications are:
Fluorotek 200 Software
Operating System
Windows 7, 8, 8.1, 10
Java Runtime Engine
Java 8 +
Bit
64 bit
Connection
USB 2.0
EnZtekEnZquik Product line:
Assay Type
Catalog #
Wavelength
E. coli
EZ-1012S
360 nm
Enterococcus
EZ-1013S
360 nm
Fecal Coliforms
EZ-1014S
490 nm
Gram Negative Aerobic Bacteria
EZ-1017S
360 nm
Salmonella
EZ-1032S
360 nm
Staphylococcus aureus
EZ-1033S
360 nm
Total Aerobic Organisms
EZ-1040S
360 nm
Total Coliforms
EZ-1041S
490 nm
MRSA
EZ-1026S
360 nm
Miscellaneous Items:
Item
Sterile Cotton Swabs
Test Vials
Calibration Standards
Replacement Light Blocking Cap

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7. Warranty
7.1. Terms
TERMS OF USE, LIMITED WARRANTY & LIABILITY WAIVER
ACCUSTRATA, INC. (“AccuStrata”) offers all of its products with the following terms and
conditions and notices as follows. By accepting and/or using anAccuStrataproduct, you hereby
acknowledge and agree to the following terms and conditions, and acceptance of these terms
and conditions are a condition precedent to any purchase/sale agreement between you and
AccuStrata.
EXCLUSIVE OBLIGATION: The AccuStrataproduct you are purchasing has been designed for
a specific use within a set of suitable operating conditions, as set forth in its User Manual, or as
indicated otherwise by AccuStrata. Any use of the AccuStrata Product for any purpose or under
any conditions, other than those specified, shall render any limited warranty void, and shall
expressly invalidate any liability of AccuStratafor damages as a result of such misuse. USER
LIMITATION: You may not modify, copy, distribute, transmit, display, perform, reproduce,
publish, license, create derivative works from, transfer, or sell, any information, software,
products or services obtained from or created by AccuStrata to any third party, without the
express written consent of AccuStrata to do otherwise. Any violation of this provision shall give
rise to an indemnification of AccuStrata by you, for any third party claims arising out of such
violation.
THIRTY (30) DAY LIMITED WARRANTY: All AccuStrata products are warranted against any
manufactured defect for a period of thirty (30) days from date of purchase, unless such product
is a custom-work for you and not a standard AccuStrata product. Any product qualifying as a
custom-work shall not be warranted against any defects for any purpose, and your acceptance
of such custom-work shall relieve AccuStrata of any liability for any purpose. WITH THE
EXCEPTION OF THE LIMITEDWARRRANTY ABOVE, YOU AGREE ANY ACCUSTRATA
PRODUCT IS PROVIDED AS IS, EXCLUSIVE OF ANY WARRANTY, INCLUDING, WITHOUT
LIMITATION, ANY IMPLIED WARRANTY OF MERCHANTABILITY, FITNESS FOR A
PARTICULAR PURPOSE, NON-INFRINGEMENT, OR ANY OTHER WARRANTY,
EXPRESSED OR IMPLIED. LIMITATION OF LIABILITY: You agree and acknowledge,
AccuStrata shall have no liability to you whatsoever for any direct, indirect, punitive,incidental,
special consequential damages arising out of or connected with the use or misuse of its
products. In particular, given the nature of AccuStrata products, you agree and acknowledge,
under no circumstances whatsoever shall AccuStrata be liable to you for any consequential
damages for damage to any non-AccuStrata product or service, arising from the failure, use or
misuse of a AccuStrata product, including, but not limited to, any vacuum system, engine,
vehicle, factory, or the like. In the event, a court of law with proper jurisdiction finds AccuStrata
liable to you for any purpose, you agree and acknowledge AccuStrata’s maximum liability shall
not exceed the purchase price of one unit of product giving rise to such liability, or $250.00,
whichever is greater.
ENTIRE OBLIGATION: These terms and conditions express the entire obligation of AccuStrata
with respect to its products. If any part of these terms and conditions are deemed void, invalid,
unenforceable or illegal, including, but not limited to, the warranty disclaimers, liability
disclaimers and liability limitations set forth above, then the unenforceable clause or sentence
may be disregarded with the remainder of these terms and conditions valid and enforced. In the

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event the unenforceable clause or sentence leaves a void in these terms and conditions, a
provision closely matching the intent of the unenforceable provision should be deemed inherent
within these terms and conditions, slightly modified to render such provision valid and
enforceable.
GENERAL: These terms and conditions are governed by the laws of the State of New Jersey,
USA. You hereby consent to the exclusive jurisdiction and venue of the Courts of New Jersey,
in all disputes arising out of or relating to the use of this product. Use of this product is
unauthorized in any jurisdiction that does not give effect to all provisions of these terms and
conditions. MODIFICATION OF TERMS AND
CONDITIONS: AccuStrata reserves the right to change the terms, conditions, and notices under
which their products are offered.

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AccuStrata Fluorotek 200 User’s Manual