Allsheng Auto-Pure Series User manual
Operation Manual
V4.0
Auto Pure Series
Nucleic Acid Purification
System
Hangzhou Allsheng Instruments Co., Ltd.
DOMINIQUE DUTSCHER SAS
Forewords
Thank you for purchasing Auto Pure series Nucleic Acid Purification System.
In order to use the instrument properly, please read carefully this manual
before operating and keep it for future reference.
Opening Check
Please check the instrument and Appendix with the packing list when you
first open the package. If you find anything missing or incorrect, please
contact the distributor.
HANGZHOU ALLSHENG INSTRUMENTS CO., LTD.
Address: Building 1&2, Zheheng Science Park, Zhuantang Town, Xihu
District,Hangzhou, Zhejiang 310024, China
Telephone: +86 571 88802738 88948289
Email: info@allsheng.com
Website: www.allsheng.com
File No.: AS153
Version: 4.0, Jul. 2020
DOMINIQUE DUTSCHER SAS
Safety Warnings and Guidelines
Warning
Please read this Manual carefully before operation.
Safety Tips
The operation, maintenance and repair of the Instrument should comply
with the basic guidelines and cautions as below. Improper use of the
instrument may cause damage to the system, inaccurate results, or
potentially nullify warranties.
Operation without reading the manual may cause damage or
even electrical shock.
Indoor use only
Read the Manual carefully before operation, only qualified and
The operator should not open or repair the Instrument without
Vendors authorization, if not, there might be cause potential
damages or injuries and affect the warranty.
DOMINIQUE DUTSCHER SAS
The Instrument should be placed in a position with low humidity,
less dust, and keep it away from water, sunshine and strong light
source. Make sure of adequate ventilation, no corrosive gases, no
strong magnetic interference and to avoid any heat sources.
Before connecting to power, make sure the voltage used is same
as the instrument required, and the maximum rated load should
be sufficient for the instrument.
Please replace the power cord with same specs if the power cord
is damaged. Please make sure theres nothing covered the power
cord and keep it away from crowds when in use.
Power off the instrument after operation and please disconnect
the plug if long time no use of the instrument and cover it with
something to prevent from dust.
Under the following circumstances, please disconnect the power
immediately and contact with your distributor.
Liquids into the Instrument;
Drenched by rain or water
During operation, the surface temperature of heating block inside
operation window could be very high. To avoid possible scald or
boiling of the liquid, do not touch the metal part when operating.
DOMINIQUE DUTSCHER SAS
3ThemaintenanceofInstrument
The inner side of drawer should be cleaned periodically by the cloth with
alcohol.
If there are any stains on the Instrument, clean them with cleansing
cream.
4Thelimitingconditionoftransportationandstorageenvironment
Ambient temperature range: 10 35
Relative humidity : 70%
Atmosphere range: 500~1060hpa
No corrosive gas and a well ventilated room.
Indicates disposal instruction.
DO NOT throw this unit into a municipal trash bin when this
unit has reached the end of its lifetime.To ensure utmost
protection of the global environment and minimize
pollution,please recycle this unit.
DOMINIQUE DUTSCHER SAS
Contents
Chapter 1 Brief Introduction...............................................................................1
Chapter 2 Specification....................................................................................2
1. Normal operating conditions..........................................................................2
2. Basic parameter & Performance.................................................................... 2
3. Overall Dimension...........................................................................................3
Chapter 3 Product Introduction......................................................................... 4
1. Structure......................................................................................................... 4
2. Operation panel..............................................................................................6
Chapter 4 Operation...........................................................................................7
1. Preparation..................................................................................................... 7
2. Connect the power......................................................................................... 8
3. Install the reagent kit......................................................................................8
4. Install the magnetic rod cover......................................................................10
5. Remove magnetic rod cover.........................................................................11
6. Operation......................................................................................................12
Chapter 5 Troubleshooting...............................................................................29
Chapter 6 Spare Parts List.................................................................................31
Chapter 7 Abbreviation and Symbols...............................................................32
DOMINIQUE DUTSCHER SAS
Auto Pure series Operation Manual Chapter 1 Brief Introduction
1
Chapter 1 Brief Introduction
Auto Pure series Nucleic Acid Purification System use the magnetic rod to
adsorb, transfer and release magnetic particles to transfer the operating
sample and in this way to purify DNA/RNA, protein and cell etc. The
Purification system able to handle 1 20,1~24,1~32 or 1 48 samples
simultaneously with special reagent kit or 96 well reaction plate, and when
using the different reagent, would be able to extract DNA/RNA from animal
or plant tissue, blood and body fluid etc.
Features
Friendly interface with easy operation
Touch screen with 3 shortcut key or mouse operation
Heating function in Lysis and elution
UV steriliza tion
Quiet operation without vibration
One stop operation to avoid contamination
Rapid extraction:10~60 minutes/time
Premium magnetic particles leads to high yield
Extendable Ethernet remote control and WiFi
Extendable APP software for mobile phone and PAD w/android system
DOMINIQUE DUTSCHER SAS
Auto Pure series Operation Manual Chapter 2 Specification
2
Chapter 2 Specification
1. Normal operating conditions
Environmental Temperature: 10 C35 C
Relative Humidity 70%
Input: AC 100 240V 50Hz/60Hz
2. Basic parameter & Performance
Table 1 Basic parameter & Performance
Model
Parameter
Auto Pure
32A
Auto Pure
48A
Auto Pure
20A
Auto Pure
20B
Auto Pure
24D
Auto Pure
24BT
Principle Magnetic Particle Method
Throughput 1~32 1~48 1~20 1~20 1~24 1~24
Kits
96
Deep well
plate
96
Deep well
plate
2ml Kits 5ml Kits 10ml Kits+
2ml tubes
5ml Kits+
2ml tubes
Sample
Volume/ L50~1000 50~1000 50~3000 50~5000 50~10000 50~5000
Stability CV 5%
Lysis temp. Ambient temperature ~120
Elution temp. Ambient temperature ~120
Heating time Heating time Ambient temperature ~120 4minutes
Temperature
Accuracy
±1
Operation
interface 7inch touch screen, 3 shortcut buttons and mouse is available
Built in protocol 8groupsofpresetprotocols,100groupsofprotocolscanbestored
Protocol
management New, Edit, Delete, Save as
Expansion
interface Standard USB, ethernet port and WIFI are available
Network Extensible Ethernet remote control, WiFi, 4G network
Pollution control UV light
Exhaust way By Fan
Data storage Available, with built in SD card
Max.input
power 450W
DOMINIQUE DUTSCHER SAS
Auto Pure series Operation Manual Chapter 2 Specification
3
Dimension
(W×D×H) 400mm×470mm×450mm 400mm×520mm×450mm
Weight kg 28kg 30kg
3. Overall Dimension
Dimension W×D×H
Auto Pure 32A 400mm×470mm×450mm
Auto Pure 48A 400mm×470mm×450mm
Auto Pure 20A 400mm×520mm×450mm
Auto Pure 20B 400mm×520mm×450mm
Auto Pure 24D 400mm×520mm×450mm
Auto Pure 24BT 400mm×520mm×450mm
DOMINIQUE DUTSCHER SAS
Auto Pure series Operation Manual Chapter 3 Product Introduction
4
Chapter 3 Product Introduction
This chapter basically introduce the instrument structure, operation buttons,
display panel as well as the preparations before operate. For the first time
user, please make sure to read this chapter before start.
1. Structure
1.1. Front
1.2. Back
Display
Shortcut key
Operation Window
Socket
Fuse
Power Switch
USB port
Ethernet port
Debu
gg
lin
g
p
ort
Fan
DOMINIQUE DUTSCHER SAS
Auto Pure series Operation Manual Chapter 3 Product Introduction
5
1.3. Auto Pure 20A/20B/24D/24BT Inside view
Note: The only difference between the experiment module of above
products is the shape of the heating strip.
1.4. Auto Pure 32A/48A Inside view
Note: The only difference between the experiment module of above
products is the shape of the heating strip.
Magnetic rod
(Over the magnetic rod
Magnetic rod cover stand
Drawer
Heating strip
UV light
(
to
p
inside
)
Magnetic rod
(Over the magnetic rod cover)
Fan
(
bottom inside
)
Ma
g
netic rod cover stand
Drawer
Heating strip
UV light
(
to
p
insid
Fan
(
bottom inside
)
DOMINIQUE DUTSCHER SAS
Auto Pure series Operation Manual Chapter 3 Product Introduction
6
2. Operation panel
Display screen: Operate by touch screen or mouse which connect with USB
port
TAB: Select for the shortcut program
RUN: Run for the shortcut program
STOP: Stop operating
Display screen
TAB
RUN
STOP
DOMINIQUE DUTSCHER SAS
Auto Pure series Operation Manual Chapter 4 Operation
7
Chapter 4 Operation
1. Preparation
Take out the instrument from packing carton and tear off the tape on the
edge of operation window, then open it and take out the foam. First please
screw out the fixed screw of position as below .Second please screw out
the screw of position then you can take out the baffle and release the
moving component.
Note: Be careful in operating or the magnetic rod will be broken.
DOMINIQUE DUTSCHER SAS
Auto Pure series Operation Manual Chapter 4 Operation
8
2. Connect the power
Connect one end of power cord to the instrument socket and the other to power (AC100~240V), then
turn on the power switch.
3. Install the reagent kit
3.1Install the customized reagent kit for Auto Pure 20A & Auto Pure 20B.
Open the drawer and mount the customized reagent kit on the heating strip, make sure the sample
well marked with 1 should be on the left side, then push back the drawer slowly.
Note: The shape of customized reagent kit of above products is different, while the installation is the
same.
3.2Install the customized reagent kit for Auto Pure 24D/24BT
Open the drawer and place the kits with sample on the kits tray, then put the kits tray to the drawer
and press the kits with hand to make sure the kits fits the heating strip well , then push back the
drawer slowly and close the door.
Customized reagent kit
DOMINIQUE DUTSCHER SAS
Auto Pure series Operation Manual Chapter 4 Operation
9
3.3Install the 96 well reaction plate on Auto Pure 32A.
Take out the drawer and put the 96 well reaction plate which already filled with samples mounted on
the locating slot, make sure the plate with chamfering should be on the left side, then push back the
drawer slowly.
Customized
reagents kits
Kits tray
drawer
door
DOMINIQUE DUTSCHER SAS
Auto Pure series Operation Manual Chapter 4 Operation
10
4. Install the magnetic rod cover
4.1Install the magnetic rod cover for 20A/20B/24D/24BT
Insert the magnetic rod cover completely on the mounting groove, and the installation quantity
depends on the reagent kit number.
96 well reaction plate
Magnetic rod cover
DOMINIQUE DUTSCHER SAS
Auto Pure series Operation Manual Chapter 4 Operation
11
4.2Install the magnetic rod cover for Auto Pure 32A/48A
Insert the magnetic rod cover completely on the mounting groove, and the installation quantity
depends on the reagent kit number.
Note: Magnetic rod cover for Auto Pure32A at most is 4pcs, for Auto Pure at most is 6pcs.The
installation method is same.
5. Remove magnetic rod cover
Press the button and take out the magnetic rod cover as per below photo.
Auto-Pure 32A
Magnetic rod cover
DOMINIQUE DUTSCHER SAS
Auto Pure series Operation Manual Chapter 4 Operation
12
6. Operation
6.1Start up Interface
Turn on the instrument and make sure the drawer is closed before start, the screen will display the
start up interface.
After start, it will enter into the shortcut mode as below:
DOMINIQUE DUTSCHER SAS
Auto Pure series Operation Manual Chapter 4 Operation
13
6.2Program Run
6.3Shortcut mode
Under the shortcut mode, select the program needed and click Run, it will enter into the program run
interface.
The program can also be selected by press Tab button on the panel and then press Run for start or
Stop for terminate.
Auto Pure 20A Running interface
DOMINIQUE DUTSCHER SAS
Auto Pure series Operation Manual Chapter 4 Operation
14
Auto Pure 20B Running interface
Auto Pure 24BT Running interface
DOMINIQUE DUTSCHER SAS
Other manuals for Auto-Pure Series
3
This manual suits for next models
6
