ALP CL-32S User manual
QC-200-S-005-1706E
AUTOCLAVE
(Steam Sterilizer)
CL- 32S / 32L
40S / 40M / 40L
Instruction Manual
● Make sure to read this manual to use this product safely.
● Place this manual in a convenient location for easy reference.
2
Contents
1. Preliminary notes P. 3
2. For the safety use P. 3 t o 6
3. Part names P.7 to 8
4. Installation P. 9 t o 1 5
5. Operation method P.16 to 38
6. Safety device P. 3 9
7. Trouble shooting P.40 to 43
8. Maintenance P.44 to 51
9. Specifications P. 5 2
10. Disposal and dismantlement P. 5 3
11. Appendix P.54 to 56
3
１．Preliminary notes
● Thank you for choosing ALP products.
Keep this manual at your hand for your quick reference whenever operating this autoclave.
● Do not use this autoclave for improper purpose. Strict observance of this manual is required.
● Please do understand and observe safety instructions written in this manual.
Failure to follow safety instructions may cause trouble and / or death.
● Following documents are attached to this autoclave. Please check and if some of them are
missing, contact local distributor.
・ Instruction Manual
・ Certificate of Quality
● The lid is not opened until putting the power on.
(Refer to ４．４ Power cable connection and protective grounding)
２．For the safety use
Preamble
Autoclave is being operated with high temperature and high pressure. Wrong installation
and /or failure to observe safety operation according to the instruction manual may cause
death or injury of operator and / or person near the autoclave, and / or destruction of
things.
Please read this chapter carefully then operate this autoclave. Important statements are
illustrated as follows. Please understand each meaning and observe these cautions.
The meaning of each illustration is as follows;
：Failure to this warning may cause death or serious injury of person.
：Failure to this caution may cause injury and / or destruction of
things.
WARNING
Prohibited
Strictly Observed
CAUTION
4
●How to use autoclave
●Installation
Do not use power strip. (Refer to ４．４ Power cable connection and protective grounding)
If power cable is bundled or tangled and /or wrongly connected, these may cause fire and / or get a shock.
Protective grounding conductor must be connected to protective grounding terminal.
Connection to gas pipe or water pipe may cause explosion, get a shock and /or breakage.
Do not install autoclave in the place where chemicals such as flammable materials, explosive
materials, and /or corrosive gas are kept.
Installing autoclave in above mentioned place may cause serious accident and/or trouble of autoclave.
●Cleaning
Do not use cleaning products for cleaning the inside of the chamber.
It may cause explosion due to corroded metallic parts.
This autoclave sterilizes objects with high temperature and high pressure.
Do not operate putting following materials and / or dangerous substances
(Refer to 11.3 Dangerous substances list) in chamber.
・Explosive materials ・Flammable materials ・Acid or Alkaline materials
・Corrosive materials ・Non heat-resistance materials
In case of sterilization of saline objects such as seawater, discharge saline water after
sterilization. Then put fresh water and operate several times without load. If you spill saline
objects, be sure to wipe off.
Failure to do them may cause explosion due to corroded chamber and pipes.
Do not disassemble and / or modify autoclave.
Autoclave being pressure vessel, disassemble and / or modification of autoclave may cause accident and / or
breakage.
Do not move autoclave holding lid nor push it hard.
Lid may jolt out of alignment which leads to serious accident and / or trouble.
Do not get autoclave wet.
If electric component get wet, it may be broken, get a shock, and even cause fire.
This autoclave should be used indoors. Do not install it outdoors.
There are many possibilities of malfunction and/or breakage of autoclave if it is used outdoors.
This autoclave must be installed on level and hard floor.
Installation without stability may cause malfunction and /or trouble.
Do not use liquid which corrode metal and/or paint (EX. thinner, hypochlorous acid, chlorine
dioxide, acetyl hydroperoxide) for cleaning the exterior.
Metallic parts may corrode.
Do not splash chlorine chemical and/or cleaning products (EX. hypochlorous acid, chlorine
dioxide, acetyl hydroperoxide) to the exterior during cleaning the floor.
Metallic parts may corrode.
CAUTION
WARNING
WARNING
CAUTION
WARNING
5
●Notes on the operation
Be sure to close lid to prevent pinching dusts before operation.
If there is something between lid and chamber which prevents lid from closing, the lid may burst at high pressure
which cause fatal accident or serious injury. Then operator may get burned by steam leaked from chamber.
Be sure to slide lid lock lever slowly to close position (left end) before operation.
If lock is not enough, operator can not start operation (Er4 is shown). But if operator can start operation by
malfunction of detection part, lid may explode at high pressure which cause fatal accident or serious injury.
Objects (including disposable plastic bag) must be put in stainless wire basket or stainless bucket
when sterilization.
If operator loads objects into chamber directly, it may cause choking pipes which may lead to serious accident.
Do not open lid forcibly during operation.
Opening lid forcibly during operation may cause fatal accident or serious injury.
Do not open lid until chamber pressure goes down to “0MPa” when operation completed or
operation interrupted.
Steam spouts out of chamber by residual pressure which may cause burn injury.
In case of sterilizing liquid objects, cool down objects enough after sterilization. Then unload
objects slowly.
Liquid objects keep high temperature after sterilization complete since temperature of liquid cool down slower than
temperature in chamber. Therefore extreme temperature change when unloading may cause high temperature liquid
spouting. It may cause burn injury.
In case of sterilizing liquid objects, metallic or heat-resistance glass container must be used.
A glass container without heat-resistance may be easy to break by extreme temperature change and operator may
be injured by this breakage.
In case of sterilizing liquid objects (EX. liquid media), do not close container with lid or bung which
has no breathability.
It may cause rise of pressure in container, because temperature in container cool down slowly. Especially glass
container is easy to explode if it is damaged.
Do not touch autoclave more than necessary.
If operator touches autoclave more than necessary, it may cause serious accident.
Do not open drain valve under following conditions.
- during operation
- chamber temperature is high
- chamber pressure is not 0MPa
It may cause burn injury by blow-out of boiling water and / or steam, water lack, and/or malfunction.
Do not touch power switch(breaker) by wet hand.
Operator may get electric shock.
Use a heat-resistance glove when operator unloads objects after operation complete.
If operator touches chamber and/or objects without heat-resistance glove, operator may get burned.
Do not touch exhaust bottle and exhaust hose during air evacuation and exhaust.
It may cause burn injury. Do not touch them until cooling down enough.
Do not touch stainless lid directly when opening or closing lid.
It may cause burn injury. Do not touch it until cooling down enough.
Genuine accessories and spare parts made by ALP Co., Ltd. must be used.
Using other company’s products may cause accident and /or breakage of autoclave.
WARNING
CAUTION
6
●Notes on power failure
In case of power failure during operation, autoclave works as follows.
(Refer to 3. Part names Operation panel about LAMP, KEY, and DISPLAY)
1. Power is cut and stop working.
2. When power is supplied again, PROCESS INDICATION BAR lights up to show the status
when power failure happened. Also, TEMPERATURE DISPLAY shows current chamber
temperature. In case of sterilization, TIMER DISPLAY shows remaining time of
sterilization, too.
3. Cool down the inside of the chamber enough.
(Press EXHAUST KEY to shorten cooling time if needed.）
4. When TEMPERATURE DISPLAY indicates 80℃, COMPLETE LAMP blinks.
5. Press STOP KEY to return to ready condition, and COMPLETE LAMP lights off.
6. After checking if pressure gauge indicates 0MPa, slide the lid lock lever slowly to open
position (right end) with pressing front part of the lid cover. Then, open lid slowly.
7. If sterilization is incomplete, set the condition of operation again then sterilize objects.
(Refer to 5. Operation method)
Do not open lid until pressure gauge indicates 0MPa and TEMPERATURE DISPLAY indicates below
80℃ when operation interrupted.
Steam spouts out of chamber by residual pressure which may cause burn injury.
Do not open drain valve under following conditions.
- chamber temperature is high
- chamber pressure is not 0MPa
It may cause burn injury by blow-out of boiling water and / or steam, water lack, and/or malfunction.
Be sure to set automatic exhaust OFF when sterilizing liquid object or glass container.
(Do not press EXHAUST KEY.)
Quick reduction of chamber pressure may cause breakage of glass container or blow-out of hot liquid.
When sterilizing liquid, it takes longer to cool. Be sure to check the temperature go down
sufficiently then unload the liquid container from chamber slowly, or you may get burned by
bumping of liquid.
Liquid objects keep high temperature after sterilization complete since temperature of liquid cool down slower than
temperature in chamber.
Use a heat-resistance glove when operator unloads objects after operation complete.
If operator touches chamber and/or objects without heat-resistance glove, operator may get burned.
Manual exhaust under 121℃ is desirable.
Exhausted steam from the chamber is not cool enough which may cause burn injury.
And water in exhaust cooling tank reduces faster therefore frequent water supply is required.
WARNING
CAUTION
7
３．Part names
Main body
Pressure gauge
Operation panel
Lid
cover
Power switch
（Breaker）
Lid lock lever
Power cable
Lid hold
(both side)
Exhaust cooling tank
(inside of the exterior)
Front caster with stopper
Exhaust bottle
Drain outlet
8
WARM
COMPLETE
TEMP
READY
PATTERN START
ENTER
TIME
STOP
EXHAUST
HEAT
EXHAUST
START
TIMER
STERILIZE
℃
MIN
O.T.
HR
MON DAY
4
15 5 1
113 181312212216172324 6 9
7210192014 8
Option
Operation panel
1. Pressure gauge
It indicates chamber pressure.
2. TEMPERATURE DISPLAY
It displays temperature in the chamber or setting
temperature. Also, it displays the date of an operation
reservation.
3. TIMER DISPLAY
It displays the remaining time to the process
completion. “down count” Also, it displays the time of
an operation reservation and error.
4. START KEY
Press it to start operation.
5. STOP KEY
Press it to stop operation on the way. Press it to reset
previous operation before starting operation.
6. PATTERN/ENTER KEY
Press it to fix an operation pattern and each setting.
7. TEMP/TIME KEY
Press it to check setting temperature, setting time
and the operation pattern. Press it to change setting
display’s blink.
8. CHANGE▲ KEY
Press it to increase the figure of the setting display.
9. CHANGE▼ KEY
Press it to decrease the figure of the setting display.
10. EXHAUST KEY
Press it to switch automatic exhaust ON and OFF or
set an automatic exhaust starting temperature. Press
it to exhaust manually.
11. START TIMER KEY
Press it to make a reservation of operation. Set an
operation starting time of month, day, hour, and
minute.
12. READY LAMP
The character lights up at the reservation condition.
The character blinks at setting change condition.
13. HEAT LAMP
The character lights up during heating or dissolving
process. The character blinks at dissolving setting
change condition.
14. STERILIZE LAMP
The character lights up during sterilizing process. The
character blinks at sterilizing setting change
condition.
15. EXHAUST LAMP
The character blinks during exhaust process.
The character lights up before exhaust process in
case of automatic exhaust ON.
16. WARM LAMP
The character lights up during warming process. The
character blinks at warming setting change
condition.
17. COMPLETE LAMP
The character lights up with the buzzer beeping at
the completion of all the processes. The character
blinks and reports whether lid can be opened when
pressing STOP KEY during operation.
PROCESS INDICATION BAR
They light up, blink, light off according to the status of
process.
18. HEATING-1 PROCESS LAMP
It blinks from start temperature of the sterilization to
the end of the air evacuation.
It blinks from start temperature of the dissolving to
the dissolving temperature.
19. HEATING-2 PROCESS LAMP
It blinks from the end of the air evacuation to the
sterilization temperature.
20. STERILIZATION PROCESS LAMP
It blinks during sterilization.
21. COOLING-1 PROCESS LAMP
It blinks to 100℃ after sterilization.
22. COOLING-2 PROCESS LAMP
It blinks from 99℃ after sterilization to the
completion.
23. WARMING PROCESS LAMP
It blinks during warming.
24． COOLING-3 PROCESS LAMP
It blinks from 99℃ after warming to the completion.
9
４．Installation
４．１ Transportation
This autoclave has casters. So it can move horizontally.
Do not pinch hands / feet when moving autoclave.
There is a risk of a fracture or bruise.
The autoclave must be moved by two or more persons.
Do not hit and /or get shock to autoclave nor hold lid cover. (Fig. 1)
It may cause breakage or damage of autoclave.
In case of lifting the autoclave, be sure to use a heavy machinery. Do not lift by humans.
It may cause a falling accident.
In case of lifting the autoclave by a forklift, be sure to insert the fork under the bottom plate
avoiding the casters. (Fig.2)
In case of lifting the autoclave by a crane, be sure to hang the bands to four corners of the
bottom plate. Put cushioning between bands and the autoclave. (Fig.3)
CAUTION
WARNING
Fig.1
Do not hold lid cover.
Fig.3
Cushioning
Fig.2
Fork
10
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４．２ Installation location
Autoclave must be installed as follows.
Refer to 4.3～4.5.
11
Do not install autoclave in the place where chemicals such as flammable materials, explosive
materials, and /or corrosive gas are kept.
Installing autoclave in above mentioned place may cause serious accident and/or trouble of autoclave.
This autoclave must be installed to a place where operator can read pressure gauge, or it may
cause serious trouble.
Do not bend exhaust hose when setting exhaust bottle, or it may cause serious accident and / or
breakage.
This autoclave must be installed on level and hard place.
Keep 10 to 15 cm space between autoclave and wall or other instruments.
Install autoclave avoiding humid and dusty place and keep away from direct sunlight.
Lock stoppers of front casters.
Do not install autoclave under electric components and alarms of the ceiling, or it may cause
malfunction of them by steam.
Be sure to use exhaust bottle, or steam from chamber is splattered to outside which may cause
burning.
Pour water to exhaust bottle up to LOW level.
If water level is under LOW level, steam spouts out directly which may cause burning.
Autoclave must not be installed outdoors.
Breakage of autoclave may happen if it is installed outdoors.
Do not use exhaust bottle for drain.
It may cause burn injury by blow-off of boiling water.
４．３ Storage / Installation condition
Autoclave must be stored / installed under following condition.
・Ambient temperature : 5 to 35℃ ・Atmospheric pressure ： 90 to 106kPa
・Relative humidity : 30 to 85％
Do not store / install autoclave locations as follows
・Outdoors
・Locations where receive direct sunlight
・Locations where autoclave get wet
・Dusty locations
・Sloping ground
・Locations with saline and /or sulfur in the air
・Locations except ambient temperature, relative humidity, and atmospheric pressure
・Locations where withstand load is lower than 150kg.
WARNING
CAUTION
12
４．４ Power cable connection and protective grounding
Lid is not opened until putting the power on. Slide lid lock lever to open position (right end)
after power on. Then open lid and unload all contents inside chamber.
(Refer to 4.5 Standard accessories)
Do not press START KEY during this operation. Otherwise contents inside chamber may
burn out due to the water lack.
Please note following points when power connection.
・Be sure to check voltage and allowable current value of outlet. Failure to connect outlet
with proper voltage and allowable current value may cause breakage.
(Refer to 9. Specifications)
・Be sure to connect the power cable to the breaker which meets below specifications.
CL-32S : AC220-240V, single phase, more than 15A
CL-32L : AC220-240V, single phase, more than 15A
CL-40S : AC220-240V, single phase, more than 20A
CL-40M : AC220-240V, single phase, more than 20A
CL-40L : AC220-240V, single phase, more than 20A
・Do not use power strip.
・When you connect power cable to distribution board directly, beware of faulty wiring and
electric shock.
・Grounding cable of the power cable must be connected to ground terminal of distribution
board.
Be sure to connect the power cable to the breaker which meets specifications of the autoclave.
Grounding cable must be connected to grounding terminal.
Do not bend and / or bundle power cable.
Improper wiring may cause fire or power leakage due to bad electrical failure or malfunction.
Do not put heavy things on power cable.
Improper wiring may cause fire or power leakage due to bad electrical failure or malfunction.
Do not connect grounding cable to gas pipe, water pipe, telephone line or lighting rod.
Improper connection may cause fire or power leakage due to bad electrical failure or malfunction.
If connector with grounding terminal is not provided, make the connector with grounding terminal.
Do not touch power connector by wet hand.
Operator may get electric shock.
Power cable must be singly-connected to the commercial power with proper voltage and
amperage.
Improper connection may cause malfunction or trouble.
WARNING
CAUTION
13
４．５ Standard accessories
① Read the cautions of sticker which covers power switch(breaker), then remove it.
② Turn on power switch, then slide lid lock lever to open position (right end) and open lid.
③ Unload all standard accessories as below.
④ Set every accessory and pour water into exhaust water cooling tank following below
instructions.
●Base plate・・・Set it on the bottom of chamber.
Purpose of use：To protect objects from direct heat generated by heater.
Level of base plate is guide for proper water level.
●Exhaust bottle・・・Set as the figure of “４．２ Installation location”.
Purpose of use：Receive steam and air in the chamber.
●Stainless wire basket・・・ Use it during operation.
Purpose of use：Load objects into it for operation.
●Chamber drain tray・・・ Use it when draining water in the chamber.
Purpose of use：Receive drain water in the chamber.
Base plate, 1pc.
Exhaust bottle(3L), 1pc.
Base plate hook, 1pc.
(CL-40L only)
Chamber drain tray, 1pc.
Stainless wire basket
CL-32S : φ30×20cm 2pcs.
CL-32L : φ30×30cm 2pcs.
CL-40S : φ38×20cm 2pcs.
CL-40M : φ38×30cm 2pcs.
CL-40L : φ38×40cm 2pcs.
14
●Base plate hook(for CL-40L only)・・・Keep it with this instruction manual.
Purpose of use：Tool for unloading base plate from bottom of chamber at the time of
maintenance.
○Pouring water into exhaust cooling tank
Mixture of steam and air in chamber is evacuated during operation.
They get cold through exhaust cooling tank, then they are evacuated to exhaust bottle.
Pour water into the tank referring to following procedure.
（Tap water is available.）
① Detach the right side panel using phillips-head screw driver.
② Pull out the plug from the funnel as figure 1.
③ Keep silicon hose straight and pour water into the tank as figure 2 on the next page.
Ideal water supply quantity : About 10L or water level of figure 3
④ Stopple the funnel.
⑤ Attach the right side panel using phillips-head screw driver.
Pouring water into the tank is not required in every operation.
However when steam is exhausted from exhaust bottle and/or water lack in the tank is
found by yearly maintenance (Refer to 8.3 Yearly maintenance), pour water following
above procedure.
Funnel
Plug
Figure 1
3 to 4cm
Figure 3
15
Be sure to set base plate during operation.
Operation without base plate may cause burning of object which leads to fire.
Do not bend exhaust hose when setting exhaust bottle, or it may cause serious accident and / or
breakage.
Do not pour too much water into exhaust cooling tank.
It makes overflow which may cause malfunction and/or breakage of autoclave.
Be sure to stopple the funnel after pouring water into exhaust cooling tank.
Water in exhaust cooling tank reduces faster therefore frequent water supply is required.
Be sure to use exhaust bottle, or steam from chamber is splattered to outside which may cause
burning.
Pour water to exhaust bottle up to LOW level.
If water level is under LOW level, steam spouts out directly which may cause burning.
Do not use exhaust bottle for drain.
It may cause burn injury by blow-off of boiling water.
WARNING
CAUTION
Ideal water supply quantity：
About 10L or 90% of tank capacity
Detail
Figure 2
Keep the silicon
hose straight.
16
５．Operation method
Be sure to read this chapter carefully and understand well before operation and use autoclave
properly, or it may cause fatal accident at worst as well as mechanical trouble.
●Flow chart of basic operation
WARNING
３. Water supply
１. Turn on power switch(breaker)
２. Close drain valve
４. Load object
５. Close and lock the lid
８. Press START KEY
７. Set operation pattern,
value of temperature and time
１０. Unload object
（below 80℃ in chamber）
１１. Close and lock the lid
９. Operation complete
６. Press STOP KEY
１２. Turn off power switch (breaker)
17
Before operation
In case of sterilization of objects such as electrolyte-containing saline (EX. sea water), be sure to
discharge water in chamber and clean it after sterilization complete. Then put fresh water and
operate several times.
Failure to maintain after sterilization may cause corrosion of bottom of chamber by electrolytic corrosion which
leads to rot.
Be sure to check if pressure gauge indicates “0MPa”.
If pressure gauge does not work properly, please contact local distributor.
If pressure gauge is wrong, it may cause serious accident and/or breakage of autoclave.
Do not bend exhaust hose when setting exhaust bottle, or it may cause serious accident and / or
breakage.
Do not touch power switch(breaker) by wet hand.
Operator may get electric shock.
５．１ Turn on power switch(breaker)
① Check if power cable is connected properly.
② Power ON.
５．２ Close drain valve
①Unload exhaust bottle from housing hole, then close drain valve.(Check if it is closed.)
Be sure to close drain valve.
If water leaks from chamber, it may cause water lack, malfunction and/or breakage of autoclave.
WARNING
CAUTION
Drain valve
Inside of the
housing hole
OPEN CLOSE
18
５．３ Water supply
5．3．1 Water supply
①Check if pressure gauge indicates “0MPa”.
②Open lid then pour water to the same level as base plate.
(CL-32S/32L : about 4L, CL-40S/40M/40L : about 6L)
Do not open lid until chamber pressure goes down to “0MPa”.
Steam spouts out of chamber by residual pressure which may cause burn injury.
Water level in camber is getting lower during operation. Check water level in every operation and
pour water to the same level if it’s under proper level.
Water put in chamber must be distilled one or purified one. Frequent cleaning of chamber is
requested when using city water which generates scale in it.
Do not spill water on the autoclave when pouring it. If you spill water, wipe up it.
Change water for sterilizing frequently. Water must be changed every day in case of daily use.
Do not use well water and industrial water which may cause corrosion and/or smudge.
Failure to use proper water may decrease life of the heating element and/or the chamber.
CAUTION
WARNING
Base plate
Heater
Proper level
Chamber
19
5．3．2 Drain water from exhaust bottle
① Unload exhaust bottle from housing hole and draw out exhaust hose from the
bottle.
② Pour water up to LOW level.
If the water level is over HIGH level, pour off the water down to LOW level.
③ Insert exhaust hose into the bottle.
The hose must not be folded when inserting. If folded, air cannot exhaust out from
the chamber and sterilization cannot be completed. Then, you cannot open the lid.
④ Insert the bottle into housing hole slowly.
Water level in exhaust bottle rises every operation. Keep the level between LOW and HIGH
at any time.
Operating over HIGH level is a cause of burn injury due to boiling over from exhaust bottle.
Operating under LOW level is also a cause of burn injury due to steam blowing.
At the time of drainage from exhaust bottle, check the bottle if it is cold enough, or it may cause
burn injury.
Exhaust bottle keeps high temperature soon after air evacuation and exhaust.
５．４ Load object
① Objects must be put in the stainless wire basket or the stainless bucket (optional
accessory), then set in the chamber slowly.
※ In case of CL-40L, use a step as below figure when loading the stainless wire
basket(optional accessory) or the stainless bucket (optional accessory) into the
chamber.
CAUTION
20
※Do not put too much objects in the chamber.
Too much objects in the chamber may cause sterilization incomplete.
（Quantity of objects to be put inside the chamber shall be less than 60% of the capacity
of chamber）
Be sure to use the stainless wire basket, the stainless wire basket with pan or the stainless
bucket.
In case of sterilization without above accessories, the chamber pressure become out of control
due to the objects choke up the pipe which leads to serious accident such as explosion.
Be sure that objects do not make contact with the inside of the lid.
If the lid is not closed properly, it may cause serious injury by blowing steam /or serious accident.
In case of sterilization of flagons such as vial container，screw cap bottle，conical flask, take out
cap or screw down it.
（Do not close mouth of flagons with impervious material such as silicon or rubber.）
In case of sterilization of liquid objects, quantity of object to be put inside container shall be less
than 80% of capacity of container.
Too much object in container may cause bumping when objects are taken out of the chamber after operation
complete.
WARNING
CAUTION
This manual suits for next models
4
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