Amersham Pharmacia Biotech INV-907 User manual

1 Introduction
Valve INV-907 is a motorised rotary 7–port
valve. The valve is used in ÄKTA™
design
chromatography systems. It is powered from
the ÄKTAdesign P-900 series system pump, and
is controlled from UNICORN™control system.
The valve has 3 positions used for:
- loading a sample loop
- injecting the sample onto the column
- washing the system pump.
The valve can also be used to switch between
upflow and downflow in a column.
Features:
• Completely swept flow path minimises
eluent or sample ”memory effect”.
• Flow rates up to 100 ml/min.
• All wetted parts are plastic PEEK.
Safety
2 Installation
Unpacking
Unpack the valve and check the items against
the packing list. Inspect the items for obvious
damage which may have occurred during
transportation.
Installing the valve
1 Connect the valve with
two UniNet cables as a
part of the UniNet 2
chain.
2 Set the ID-switch at the
bottom to
the required valve number 0–9. The
number should correspond to that used in
UNICORN.
Note: All valves must have different
numbers.
3 Mount the valve vertically, in the way it
is shown in the user documentation of
your ÄKTAdesign chromatography
system.
4 Apply an identification number from the
labels supplied. The number should be the
same as that of the rotary switch at the
bottom of the valve. Place the number so
that it is easily readable from the front.
5 Connect the tubing.
CAUTION! Before connecting Valve
INV-907 ensure the power is switched OFF
at the system pump or the complete system.
WARNING! When using hazardous
chemicals, all suitable protective measures,
such as protective glasses, must be taken.
INV-907
3
Instructions
Valve INV-907
56-8101-01
Edition AC
UniNet 2 network
56-8101-01_AC.qxd 1998-10-26 11:45 Sida 1 (1,1)

6When used as an injection valve connect
the tubing as follows:
Port Connect to
1 Column inlet
2 Sample loop or bottom tubing
of Superloop
3 Injection port
4 Waste
5 Waste
6 Sample loop or top tubing of
Superloop
7 Outlet of the pump
7 When used as an upflow/downflow valve,
connect the valve as indicated in the user
documentation of your ÄKTAdesign
chromatography system.
3 Operation
The valve is controlled from UNICORN.
When used as an injection valve use
the instruction InjectionValve in
System Control:Manual:Flowpath. The valve can
be set in 3 positions:
• LOAD position 1 (home position)
• INJECT position 2
• WASTE position 3
If the valve has not been activated it is in
position 1.
1
2
3
45
6
7
1
2
3
45
6
7
1
2
3
45
6
7
Column
LOAD, position 1
Sample
syringe
Waste Waste
Pump
Column
INJECT, position 2
Waste
Pump
Column
WASTE, position 3
Waste
Pump
Valve INV-907
Instructions 56-8101-01 Edition AC
2
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Manual sample loading
The sample loop and Superloop can be loaded
with a syringe. Connect Superloop with the
bottom tubing connected to port 2 and the
upper tubing to port 6.
For large volumes use a luer
female/1/16” male union
connected to port 3.
For smaller volumes use an
injection needle together with
the injection fill port as
described below:
Check the fill port nozzle is screwed into
the fill port.
Note: The thread is left-handed.
1 Loosely thread the fill port screw into
valve port 3.
2 Insert the injection needle (0.7 mm o.d.)
into the injection fill port.
3 Tighten the fill port until the nozzle has
formed a seal around the needle tip. When
the seal is adjusted correctly, it feels as if
you are penetrating a septum at the end of
the injection fill port. The seal should
provide easy insertion and removal of
the needle.
4. Mount the syringe holder on the fill port.
Two techniques can be used for filling
the sample loop; partial or complete filling.
Type of filling Volume to load
Partial filling max 50% of the sample loop
volume
Complete filling 2-5 times the sample loop
volume
Partial Filling
Partial filling is used when high recovery is
required. Partial filling allows the injected
volume to be changed without changing
the loop and does not waste sample.
Partial filling is achieved as follows:
1 Set injection valve to position LOAD.
2 Load the syringe with a large volume of
buffer (5x the loop volume).
3 Fill the sample loop carefully with buffer.
4 Set the injection valve to position INJECT
before taking out the syringe.
Note: If the syringe is taken out when
the injection valve is in position LOAD,
self drainage will occur and air will enter
the sample loop.
5 Load the syringe with the required volume
of sample. No more than half (50%) a
loop volume of sample should be loaded
into the loop.
6 Insert the syringe in position 3 on
the injection valve. Set the injection valve
to position LOAD.
Note: Do not load the sample before
the valve is in position LOAD.
7 Gently load the syringe contents into
the loop.
8 Leave the syringe in position. The sample
will be injected onto the column when
the valve is switched to INJECT in
the method.
Valve INV-907
Instructions 56-8101-01 Edition AC 3
INV-907
3
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Valve INV-907
Instructions 56-8101-01 Edition AC
4
Complete filling
In this method a surplus of sample is used to
ensure that the sample loop is filled
completely, allowing reproducible sample
volumes. In preparative applications
the sample volume should be at least 2 times
the volume of the sample loop. For analytical
reproducibility a sample volume 5 times
the volume of the sample loop should be used.
With complete filling the sample volume can
only be changed by changing the loop size.
Complete filling is achieved as follows:
1 Set valve to position LOAD.
2 Load the syringe with sample (2-5 times
the loop volume).
3 Gently load the syringe contents into
the loop.
4 Leave the syringe in position. The sample
will be injected onto the column when
the valve is switched to INJECT in
the method.
Note: If the syringe is taken out before
the sample is injected onto the column, self
drainage will occur and the loop will be
emptied.
Emptying the sample loop
When emptying the sample loop a buffer
volume of approx. 5 times the sample loop
volume should be used to flush the loop and
ensure that all sample is injected onto
the column.
Storage
Overnight: The valve can be left filled with a
buffer.
Weekend or Long time storage: Flush the valve
with water and then fill it with 20% Ethanol.
4 Maintenance
Period Action
Every 12 months Change channel plate and
or when required distribution plate
Cleaning-in-place
Pump a cleaning or sanitizing agent through
the valve. The standard recommendation is to
pump 1 M NaOH for 30 minutes and then
wash out with buffer.
Changing channel plate and
distribution plate
A replacement kit, Valve Kit INV–907, is
available, see ordering information in
Reference Information.
1 Ensure that the valve is in position 1 and
then disconnect it from the pump.
2 Remove the 4 screws on the front using
the supplied 3 mm Allen key. Loosen each
one equally in turn so the distribution
plate comes off parallel to the valve body.
CAUTION! Only spare parts approved or
supplied by Amersham Pharmacia Biotech
may be used for maintaining and servicing
the valve.
3
INV-907
Distribution
plate
Channel
plate
WARNING! NaOH is injurious to
health. Avoid spillage.
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Valve INV-907
Instructions 56-8101-01 Edition AC 5
3Slide the screws out.
4 Remove the distribution plate containing
the ports.
5 Remove the old channel plate and insert a
new one.
6 Remount a new distribution plate so that
the text 3is horizontal and to the right of
the central tubing connection. Using
the Allen key, tighten the 4 screws in turn,
a little at a time, until the distribution
plate is fixed to the valve body.
5 Trouble shooting
If the suggested actions do not correct the fault,
call Amersham Pharmacia Biotech.
Fault Action
The valve is not switching
1 Check the connection to the pump.
The valve should be connected to
the
UniNet 2 socket, not
the UniNet 1 socket.
2 Check the ID-switch on the valve.
The ID number should correspond
to the number set in UNICORN.
3 Check the
UniNet cable and
replace if required.
The valve is switching to wrong position
The valve parts may have been
incorrectly assembled after
replacement.
1 Check that the distribution plate
marking 3is horizontal.
External leakage
1 Check the tubing connections.
Tighten or replace if required.
Internal leakage Internal leakage are easy to detect
at the small hole on the underside
of the valve body.
1 Internal valve parts may be worn.
Change channel plate and
distribution plate according to
section 4.
High back pressure (clogging)
1 Do cleaning-in-place according to
the instructions in section 4.
2 Change channel plate and
distribution plate according to
section 4.
Other faults Contact Amersham Pharmacia
Biotech.
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Valve INV-907
Instructions 56-8101-01 Edition AC
6
Reference information
Description
The valve consists of two main parts:
• Housing which
encloses
the motor and
electronics.
• Valve body with
a rotating
central core.
As the channel
plate is turned by
the motor, different ports are connected.
Valve switching is controlled from
UNICORN by reading the actual position of
the channel plate.
The geometry of the valve assures that
the flow path is completely swept so that
solvent or sample ”memory effect” is virtually
non-existing. The valve rotates the shortest
way to the next position (max. 180°).
The material used in the switching parts
ensures both long mechanical and chemical
lifetime.
The valve housing contains no user
replaceable items.
Technical specifications
Operating data
Max Flow rate 100 ml/min
Max Pressure 25 MPa (250 bar, 3600 psi)
Back pressure <50 kPa at 100 ml/min with water
Leakage < 0.1 µl/min at 25 MPa
pH stability range 1–13, 1–14 (<1 day exposure)
Viscosity Max. 5 cP
Switch time <260 ms between two
adjacent positions
Operating life time >50 000 cycles, two
adjacent positions
Environment +4 to +40 °C
20-95% relative humidity
84-106 kPa (840-1060 mbar)
atmospheric pressure
Physical data
Internal volume
Pos 1 LOAD
Port 1-7 9 µl
Port 2-3 5 µl
Port 4-6 8 µl
Pos 2 INJECT
Port 1-2 7 µl
Port 3-5 6 µl
Port 6-7 9 µl
Pos 3 WASH
Port 2-4 8 µl
Port 5-7 9 µl
Flow channel
diameter 0.8 mm
Valve principle Motor controlled valve
Functions Switching 3 positions controlled
from UNICORN
Degree of
protection IP 43
Wetted materials PEEK (polyetheretherketone)
Chemical
resistance The wetted parts are resistant to
organic solvents and salt buffers
commonly used in chromatography
of biomolecules, except
100% ethylacetate, 100% hexane
and 100% tetrahydrofuran (THF)
Power requirement
32 V DC ±10% from the system
pump
Power consumption
Up to 9 W
UniNet 2 address 0–9
Inlet and outlet tubing
UNF 10-32 2B ”Fingertights”
for capillary tubing 1/16”
outer diameter
Dimensions,
H x W x D 135 x 80 x 120 mm
Weight 1.2 kg
EMC Standards This product meets the
requirement of the EMC Directive
89/336/EEC through the
harmonized standards
EN 50081-1 (emission) and
EN 50082-1 (immunity)
Note: The declaration of
conformity is valid for the
instrument when it is
• used in laboratory locations
• used in the same state as it
was delivered from Amersham
Pharmacia Biotech except for
alterations described in the
user manual
• connected to other CE labelled
Amersham Pharmacia Biotech
instruments or other products
as recommended.
3
INV-907
Housing
Valve
body
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Valve INV-907
Instructions 56-8101-01 Edition AC 7
Accessories and spare parts
Item Quantity Code no.
per pack
Valve INV-907 including
one UniNet cable
(fill port, needle and
syringe holder are
not included) 1 18-1108-40
Injection kit INV-907
including fill port
needle and syringe
holder 1 18-1110-89
Valve kit INV-907
including channel plate
and distribution plate 1 18-1109-05
Sample loops
100 µl 1 18-1113-98
500 µl 1 18-1113-99
1 ml 1 18-1114-01
2 ml 1 18-1114-02
Superloop, 10 ml 1 18-1113-81
Superloop, 50 ml 1 18-1113-82
Superloop, 150 ml 1 18-1023-85
Cable UniNet, 0.7 m 1 18-1109-74
Mounting bracket 1 18-1109-11
Teflon tubing, i.d. 1/8",
o.d. 3/16" 3 m 18-1112-47
Tubing connector for 3/16"
o.d. tubing 10 18-1112-49
Ferrule for 3/16" o.d.tubing 10 18-1112-48
Stop plug, 5/16” 5 18-1112-50
Stop plug, 1/16" 5 18-1112-52
Union Luer
female/1/16" male 2 18-1112-51
Union
1/16" female/M6 male 6 18-1112-57
Union
M6 female/1/16" male 8 18-1112-58
PEEK tubing, i.d. 0.75 mm,
o.d. 1/16" 2 m 18-1112-53
Teflon tubing, i.d. 0.75 mm,
o.d. 1/16" 2 m 18-1112-54
PEEK tubing, i.d. 1.0 mm,
o.d. 1/16" 2 m 18-1115-83
Fingertight connector 1/16" 10 18-1112-55
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Important user information
WARNING!
The Warning sign is used to call attention to the
necessity to follow an instruction in detail to avoid
personal injury. Be sure not to proceed until the
instructions are clearly understood and all stated
conditions are met.
CAUTION!
The Caution sign is used to call attention to
instructions or conditions that shall be followed to
avoid damage to the product or other equipment. Be
sure not to proceed until the instructions are clearly
understood and all stated conditions are met.
Note
The Note sign is used to indicate information
important for trouble-free or optimal use of the
product.
Should you have any comments on this instruction, we
will be pleased to receive them at:
Amersham Pharmacia Biotech
SE–751 84 Uppsala
Sweden
Trademarks
ÄKTA and UNICORN are trademarks of Amersham
Pharmacia Biotech Limited or its subsidiaries.
Amersham is a trademark of Nycomed Amersham plc.
Pharmacia and Drop Design are trademarks of
Pharmacia & Upjohn Inc.
Terms and Conditions of Sale
All goods and services are sold subject to the terms
and conditions of sale of the company within the
Amersham Pharmacia Biotech group which supplies
them. A copy of these terms and conditions of sale is
available on request.
Adresses
Amersham Pharmacia Biotech UK Limited
Amersham Place
Little Chalfont
Buckinghamshire
England HP7 9NA
Amersham Pharmacia Biotech AB
SE-751 84 Uppsala
Sweden
Amersham Pharmacia Biotech Inc
800 Centennial Avenue
PO Box 1327
Piscataway
NJ 08855 USA
© Amersham Pharmacia Biotech AB 1998
– All rights reserved
TC information ab, Uppsala. Printed in Sweden by TK i Uppsala AB, October 1998
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