Ankom XT15 User manual

2052 O’Neil Rd, Macedon NY 14502
Telephone: [phone]
Fax: [phone]
www.ankom.com
Operator’s Manual
Rev F 5/9/17

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Table of Contents
Introduction ..................................................................................................................................................................5
Warranty.......................................................................................................................................................................5
Filter Bags .....................................................................................................................................................................5
Operating Environment ................................................................................................................................................5
Contact Information .....................................................................................................................................................6
Instrument Description.................................................................................................................................................7
Safety Precautions ........................................................................................................................................................8
Instrument Installation .................................................................................................................................................9
Fat Extraction Calculations .........................................................................................................................................10
Fat Extraction Support Items ......................................................................................................................................10
Sample Preparation ....................................................................................................................................................11
Fat Extraction step-by-step procedure using the ANKOMXT15 ....................................................................................17
Periodic Maintenance.................................................................................................................................................21
Troubleshooting & Replacement Parts.......................................................................................................................23
Diagnostics..................................................................................................................................................................24
Appendix A –Electrical Diagram (pg. 1 of 3) ..............................................................................................................29

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Operator’s Manual
Rev F 5/9/17 pg. 5
Introduction
ANKOM Technology designs, manufactures, and markets instruments and support products used by analytical
laboratories around the world in the environmental, agricultural, biomass, and food industries. ANKOM
Technology can provide you with products for determining or monitoring dietary fiber, detergent fiber, fat,
digestibility, microbial fermentation (anaerobic or aerobic) and more.
Committed to Total Customer Satisfaction, ANKOM designs every product based on a thorough assessment of
customer needs.
Congratulations on your purchase of the ANKOMXT15 Extraction System.We are confident that this product will
effectively serve your needs.
The ANKOMXT15 Extraction System was designed to automate the process of fat extraction. This reduces
technician variation, increasing precision. By carefully following the operating instructions in this manual you will
understand the details of sample and filter bag handling as well as the instrument controls, helping you to achieve
the best possible results.
Please review the entire contents of this manual before you begin operating this
product.
Warranty
ANKOM Technology warrants the ANKOMXT15 Extraction System against any defects due to faulty workmanship
or material for one year after the original date of purchase. This warranty does not include damage to the instrument
resulting from neglect or misuse. During the warranty period, should any failure result from defects in
workmanship or materials ANKOM Technology will, at its discretion, repair or replace the instrument free of
charge.
Extended warranties are available upon request.
Filter Bags
Use only ANKOM Technology filter bags (part # XT4) in your ANKOMXT15 Extraction System. Use of any other
filter mechanism will void the warranty. Filter bags can be purchased from ANKOM Technology or from your local
authorized ANKOM distributor.
Operating Environment
Your ANKOMXT15 Extraction System is designed to operate within the following environments:
Ambient Temperature Range: 15°−35°C
Power: 100V−120V ~ 50/60Hz 10A
220V−240V ~ 50/60Hz 5A

Operator’s Manual
pg. 6 Rev F 5/9/17
Contact Information
At ANKOM Technologywe are committed to your total satisfaction and therefore always available to help you get
the most from your ANKOM products. We are also very interested in any comments or suggestions you may have
to help us improve.
For any questions or suggestions regarding your instrument, please contact us at:
Telephone: [phone]
Fax: [phone]
Email: [e-mail]
www.ankom.com

Operator’s Manual
Rev F 5/9/17 pg. 7
Instrument Description
General Description
The ANKOMXT15 Extraction System is designed to extract Crude (Free) Fat using conventional solvents, typically
petroleum ether. The compounds extracted are primarily triacylglycerols together with a small quantity of related
lipids, traditionally termed “Crude Fat.” The analysis is achieved by measuring the loss of mass due to the
extraction of fat/oil from the sample encapsulated in a filter bag. The quantitative isolation of the sample is
accomplished by surrounding the sample in a sealed filter bag with a filtering capacity in the 2 to 3 micron range.
The filter bag has sufficient porosity to permit rapid solvent passage and is composed of polymeric material that is
resistant to the higher temperatures and solvents used in the instrument.
The quantitative isolation achieved by the filter bag permits the ANKOMXT15 Extraction System to automatically
process samples in batches of up to 15. With the solvent reservoir filled, simply place filter bags with sample in the
Extraction Vessel, lock the vessel in place, select the extraction time and temperature, and press “Start.” The
instrument will automatically fill the Extraction Vessel with solvent, extract the fat from the sample, and recycle the
solvent.
Below is a detailed view of the ANKOMXT15 Extraction System.
Keypad
Display
Power Switch (not visible)
Safety Valve Handle
Pressure Gauge
Extraction Vessel
Solvent Fill Port
Main Handle
Solvent Sight Level
Reflux Water Flow Control

Operator’s Manual
pg. 8 Rev F 5/9/17
Safety Precautions
Flammable Hazard: Static electricity is to be avoided as it is a spark hazard.
Hot Surfaces: Do not touch the extraction vessel during operation. The surface
can exceed 70°C (158°F). Failure to observe this caution may result in injury.
Hazardous Voltages: Do not operate the instrument with the electrical
compartment open. Hazardous voltages are present during operation. Failure to
observe this caution may result in electrical shock or electrocution.
Hazardous Materials: The ANKOMXT15 Extraction System comes equipped with
a charcoal filter which traps any residual vapor in the vent system. By properly
maintaining the charcoal filter, volatiles will not escape the vent system. We
recommend replacement of the filter every 3 months or after 100 extraction runs.
Trace amounts of vapor may also be present when samples are removed. It is
recommended that the ANKOMXT15 Extraction System be placed in a well
ventilated environment, away from any heat or ignition sources. Organic
solvents may be flammable. Follow safe laboratory practices according to both
local and federal regulations when installing this instrument and when handling
any organic solvents.
WARNING: Attempts to override safety features or to use this instrument in a
manner not specified by ANKOM Technology voids the warranty and may result
in serious injury or even death.
This system is designed to meet and/or exceed the applicable standards of CE,
CSA, NRTL and OSHA.
Please review the entire contents of this manual before you begin operating this
instrument.

Operator’s Manual
Rev F 5/9/17 pg. 9
Instrument Installation
Site Requirements
To install and operate the ANKOMXT15 Extraction System you will need the following:
Adjustable wrench
Chilled or tap water supply not to exceed 23°C
Adequate power (see “Operating Environment” section)
Drain
Instrument Installation Procedure
To install the ANKOMXT15 Extraction System, follow the procedure detailed below.
1.
Remove the instrument from the shipping container and place in an area that is free of dust and excessive
moisture, and is well ventilated (away from any heat or ignition sources).
Because the ANKOMXT15 Extraction System is a closed system, there is very
limited exposure to solvents. When used in conjunction with a charcoal filter on
the vent line the instrument produces less than 1ml of volatiles. It is the user’s
responsibility to determine whether venting of this instrument requires placement
in a hood. Please follow both local and federal regulations when installing this
instrument.
2.
Connect the tube from the top fitting to the water supply and the one from the bottom fitting to the drain.
Ensure that there are no kinks or bends in any of the tubing connected to the
instrument.
3.
Connect the vent flask to the Vent Flask Connector on the instrument and run the tubing to the inline
charcoal filter. Periodically check the vent line to verify that it is free from any blockage or kinks.
Generally, there should not be any significant solvent in the vent flask. Any solvent visible in the flask
may indicate a problem with the water supply to the instrument.
Change the Charcoal Filter every 3 months or after 100 extraction runs.
4.
Plug the power cord into a grounded receptacle.
Charcoal Filter
Water Supply
Drain
Vent Flask
Connector

Operator’s Manual
pg. 10 Rev F 5/9/17
Fat Extraction Calculations
Crude Fat Calculation
Crude Fat contained within a food or feed sample can be calculated using the following formula:
% Crude Fat
=
100 x (W2–W3)
W1
Where:
W1
=
Original weight of sample
W2
=
Weight of pre-extraction dried sample and filter bag
W3
=
Weight of dried sample and filter bag after extraction
For Liquid Samples, W2and W3in the equation above will include the weight of Diatomaceous Earth (DE).
For Meat Samples with > 15% fat and plant samples with > 20% fat:
% Crude Fat
=
100 x ((W2–W4) –W3)
W1
Where:
W4
=
Weight of the weigh tin
Total Fat Calculation
If hydrolysis is done prior to fat extraction, then Total Fat contained within a food or feed sample can be calculated
using the following formula:
% Total Fat
=
100 x (W2–(W3+ (C1–C2)))
W1
Where:
W1
=
Original weight of sample
W2
=
Weight of dried sample, filter bag, and Diatomaceous Earth (DE) after hydrolysis
W3
=
Weight of dry extracted sample, filter bag, and DE
C1
=
Blank filter bag dry weight after hydrolysis
C2
=
Blank filter bag weight after extraction
The Hydrolysis procedure can be found in the ANKOM HCl Operator's Manual.
Fat Extraction Support Items
The following support items are needed to perform the fat extraction procedure:
Item
Recommended Product
Electronic Balance with four-place readout
ANKOM #TB Balance Hardware
ANKOM #TBS Balance Software
Filter Bags
ANKOM #XT4
Bag Holder (used for adding sample to an empty filter bag)
ANKOM #101.2
Heat Sealer for sealing the filter bags
ANKOM #HS (120V), #HSi (220V)
Solvent Resistant Marker
ANKOM #F08
Desiccant Pouch or Weigh Tin Desiccator for drying
ANKOM #X45, #X49
Diatomaceous Earth (DE) - used with liquid samples
ANKOM #DE1, DE2
Dryer (capable of temperatures from 60-120°C) - optional
ANKOM #RD (120V), #RDI (220V)
Oven for drying (capable of maintaining 102°C±2°)
Ventilated oven trays to allow for sufficient oven air flow
Weigh Tins (used with samples of > 20% fat)
Sample
Spoon

Operator’s Manual
Rev F 5/9/17 pg. 11
Sample Preparation
Sample Preparation Procedure for Crude Fat –f fo or r L LO OW W f fa at t s sa am mp pl le es s
To prepare meat samples with ≤ 15% fat by weight or plant samples with ≤ 20% fat by weight for fat extraction,
follow the procedure detailed below.
1.
Using a Solvent Resistant Marker, number all of the empty filter bags you will
use during the fat extraction procedure. Although it is not necessary in the
determination of fat, it is recommended that you weigh and record the weights of
all the filter bags prior to filling them in order to calculate the moisture value for
the samples.
% Moisture = 100 x ((Filter Bag Weight + Sample Weight) - Weight after drying)
Sample Weight
Measuring moisture can also help verify the operation of your drying oven.
2.
Place an empty filter bag in the Bag Holder in an open position.
3.
Tare the weight of the empty filter bag and the holder together.
4.
Add 1.5 –2g of sample to the filter bag. Keep all particles away from the sealing
area of the filter bag.
5.
Record the weight of the sample (W1).
6.
Set the Heat Sealer dial to 6. (The setting may vary from sealer to sealer.)
7.
Seal the filter bag within 4mm of its open end. Keep the
sealer arm down for 2 –3 seconds after the red sealer light
turns off (to cool the seal). The seal can be seen as a solid
melted stripe along the top edge of the filter bag (as shown
to the right). If the seal is not strong, re-seal the bag.
8.
Repeat steps 1 –7 for all filter bags that will be used in the ANKOMXT15 Extraction System.
(Up to 15 bags can be processed during one procedure.)
9.
Dry all samples at 102°C±2° for 3 hours to remove moisture prior to the
extraction.
10.
Remove the samples from the oven and place them in a Desiccant Pouch.
11.
Allow the samples to cool to room temperature. This should take about 10 –15
minutes.
12.
Re-weigh each bag (W2) immediately after removing from the Desiccant Pouch.
The samples are now ready for the fat extraction procedure.
Seal

Operator’s Manual
pg. 12 Rev F 5/9/17
Sample Preparation Procedure for Crude Fat –f fo or r H HI IG GH H f fa at t s sa am mp pl le es s
To prepare meat samples with > 15% fat by weight or plant samples with > 20% fat by weight for fat extraction,
follow the procedure detailed below.
1.
Using a Solvent Resistant Marker, number all of the weigh tins and empty
filter bags you will use during the fat extraction procedure. Use one weigh tin
per filter bag. Although it is not necessary in the determination of fat, it is
recommended that you weigh and record the weights of all the filter bags
prior to filling them in order to calculate the moisture value for the samples.
% Moisture = 100 x ((Filter Bag Weight + Sample Weight) - (Weight after drying - Tin Weight))
Sample Weight
Measuring moisture can also help verify the operation of your drying oven.
2.
Dry all weigh tins in an oven at 102°C±2° for 15 minutes.
3.
Remove weigh tins from the oven and place them in a
Weigh Tin Desiccator or in a Desiccant Pouch.
4.
Allow weigh tins to cool to room temperature.
5.
Weigh and record tin weights (W4).
6.
Place an empty filter bag in the Bag Holder in an open position.
7.
Tare the weight of the empty filter bag and the holder together.
8.
Add 1.5 –2g of sample to the filter bag. Keep all particles away from the
sealing area of the filter bag.
9.
Record the weight of the sample (W1).
10.
Set the Heat Sealer dial to 6. (The setting may vary from sealer to sealer.)

Operator’s Manual
Rev F 5/9/17 pg. 13
11.
Seal the filter bag within 4mm of its open end. Keep the
sealer arm down for 2 –3 seconds after the red sealer light
turns off (to cool the seal). The seal can be seen as a solid
melted stripe along the top edge of the filter bag (as shown
to the right). If the seal is not strong, re-seal the bag.
12.
Repeat steps 1 –11 for all filter bags that will be used in the ANKOMXT15 Extraction System.
(Up to 15 bags can be processed during one procedure.)
13.
Place the sealed filter bags in their corresponding weigh tins for pre-drying.
14.
Dry all samples at 102°C±2° for 3 hours to remove moisture prior to the extraction.
15.
Remove the weigh tins and filter bags from the oven and place them together
in a Weigh Tin Desiccator.
A Weigh Tin Desiccator is recommended because it will hold between 10 and 14
weigh tins with filter bags, depending on the size of the weigh tins. A Desiccant
Pouch will hold about 4.
16.
Allow the samples to cool to room temperature. This should take about 10 –15 minutes.
17.
Re-weigh and record the weights of each weigh tin (W4) and filter bag (W2) immediately after removing
from the Weigh Tin Desiccator.
The samples are now ready for the fat extraction procedure.
Seal

Operator’s Manual
pg. 14 Rev F 5/9/17
Sample Preparation Procedure for Crude Fat –f fo or r L Li iq qu ui id d s sa am mp pl le es s
To prepare liquid samples for fat extraction, follow the procedure detailed below.
1.
Using a Solvent Resistant Marker, number all of the empty filter bags you will
use during the fat extraction procedure. Although it is not necessary in the
determination of fat, it is recommended that you weigh and record the weights of
all the filter bags prior to filling them in order to calculate the moisture value for
the samples.
% Moisture = 100 x ((Filter Bag Weight + Sample Weight) - Weight after drying)
Sample Weight
Measuring moisture can also help verify the operation of your drying oven.
2.
Place an empty filter bag in the Bag Holder in an open position.
3.
Tare the weight of the empty filter bag and the holder together.
4.
Add Diatomaceous Earth (DE).
4.1 Add 1.0 –1.25 g of DE to the filter bag.
4.2 Using a pencil eraser (or similar sized utensil) make an indentation or a
space in the DE into which the liquid sample can be added using a pipette.
4.3 Record the weight and tare.
5.
Pipette 1.5 –2 g of liquid sample into the DE indentation in the filter bag.
(Do NOT use more liquid sample than can be absorbed by the DE.)
6.
Record the weight of the sample (W1).
7.
Set the Heat Sealer dial to 6. (The setting may vary from sealer to sealer.)
8.
Seal the filter bag within 4mm of its open end. Keep the
sealer arm down for 2 –3 seconds after the red sealer light
turns off (to cool the seal). The seal can be seen as a solid
melted stripe along the top edge of the filter bag (as shown
to the right). If the seal is not strong, re-seal the bag.
9.
Repeat steps 1 –8 for all filter bags that will be used in the ANKOMXT15 Extraction System.
(Up to 15 bags can be processed during one procedure.)
Seal

Operator’s Manual
Rev F 5/9/17 pg. 15
10.
Dry all samples at 102°C±2° for 3 hours to remove moisture prior to the extraction.
11.
Remove the samples from the oven and place them in a Desiccant Pouch.
12.
Allow the samples to cool to room temperature. This should take about 10 –15
minutes.
13.
Re-weigh each bag (W2) immediately after removing from the Desiccant Pouch.
The samples are now ready for the fat extraction procedure.

Operator’s Manual
pg. 16 Rev F 5/9/17
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Operator’s Manual
Rev F 5/9/17 pg. 17
Fat Extraction step-by-step procedure using the ANKOMXT15
To perform fat extraction on prepared samples, follow the procedure detailed below.
1.
Turn the instrument power ON.
The Display will briefly show the software version number followed
by the screen to the right.
The factory default solvent shown on the Display is "Pet Ether." However, the
instrument computer will store in memory the last solvent selection made and use
it as the default solvent shown on the Display screen every time the instrument is
ready for a new run.
2.
Select the solvent you want to use.
2.1 Press the Down Arrow (v) key on the Keypad until the desired solvent is shown on the Display.
2.2 Fill the solvent reservoir as needed.
2.21 Unscrew the Fill Cap.
2.22 Pour solvent into the Fill Port up to the dark blue level mark
on the Solvent Sight Level. (About 500 ml of solvent is
needed to reach the dark blue level mark.) Do not add solvent
up to the top of the Solvent Sight Level tube or it will start
flowing into the Vent Flask.
If using Diethyl Ether or a solvent mixture that includes Diethyl Ether (such as the
Mojonnier Mixture), you will need the O-ring Kit for High Penetration Solvents
(part # X95) to replace the standard O-rings on the top of the Extraction Vessel
and the Valve Kit for High Penetration Solvents (part # X55).
Warning: Do NOT use Acetone in this instrument because it will cause damage
to the valves.
2.3 Press ENTER on the Keypad to complete the solvent selection.
The Display should now show the screen to the right.
Remove/Insert
Samples <ENTER>
PET Ether
v <ENTER>

Operator’s Manual
pg. 18 Rev F 5/9/17
3.
Insert Filter Bags into the instrument.
3.1 Press down the Safety Valve Handle to release the Safety Valve
Pin and rotate the Main Handle upward until it stops.
3.2 Use the Extraction Vessel Handle to pull the Extraction Vessel
out.
3.3 Slide the Extraction Vessel toward you far enough to remove the
white PTFE Insert or to put the Bag Holder directly into the
PTFE Insert while it remains in the Extraction Vessel.
It is not necessary to remove the white PTFE Insert from the Extraction Vessel in
order to put the Bag Holder in place.
3.4 Put the Bag Holder (with up to 15 bags) into the PTFE Insert.
3.5 If you removed the PTFE Insert, put it back
into the Extraction Vessel, aligning the PTFE
Insert slot with the Extraction Vessel pin.
The PTFE Insert O-ring and the Extraction Vessel O-ring must be in
place before running the instrument.
The PTFE Insert slot must be aligned with the Extraction Vessel pin
before running the instrument.
PTFE Insert O-ring
Extraction Vessel O-ring
PTFE Insert
slot and
Extraction
Vessel pin
PTFE Insert
Bag Holder

Operator’s Manual
Rev F 5/9/17 pg. 19
3.6 Slide the Extraction Vessel into the instrument ensuring the
Extraction Vessel O-ring stays in place.
3.7 Pull the Main Handle downward, closing and locking the Extraction
Vessel in place. You will hear a click noise when the Safety Valve
Pin locks in place.
Before running the instrument, verify that the Extraction Vessel is locked in
place by attempting to rotate the Main Handle upward. If it is not locked,
repeat step #8 above.
3.8 Press ENTER on the Keypad.
The Display should now show the screen to the right. (The
time shown will be the last time entered.)
4.
Set the Extraction Time.
4.1 Using the arrow keys on the Keypad, select the desired Extraction time. (The recommended time is
60 minutes.)
4.2 Press ENTER on the Keypad to set the Time.
The Display should now show the screen to the right. (The
temperature shown will be the last temperature entered.)
5.
Set the Extraction Temperature.
5.1 Using the arrow keys on the Keypad, select the desired Extraction Temperature. (The
recommended maximum temperature is 90°C.)
5.2 Press ENTER on the Keypad to set the Temperature.
The Display should now show the screen to the right.
6.
Verify that the water supply is on.
7.
Run the instrument.
Press START on the Keypad.
The instrument will run through its automatic operation, showing
its status on the Display. When the instrument has completed its
automatic operation, the Display will show the screen to the right.
At this time, the only light shining on the Display should be the green
power light.
PET Ether
v <ENTER>
Close Vessel
Water On <START>
Select temp 90
(^v)... <ENTER>
Select time 60
(^v)... <ENTER>

Operator’s Manual
pg. 20 Rev F 5/9/17
8.
Determine the amount of fat that was extracted.
8.1 When the Pressure Gauge reads zero, remove the Extraction Vessel by pushing down on the Safety
Valve Handle to release the locking pin and then rotating the Main Handle to the open position.
8.2 Use the Extraction Vessel Handle to remove the Extraction Vessel.
Warning: The Extraction Vessel interior and top will be HOT when the
extraction process is complete.
8.3 Remove the PTFE Insert from the Extraction Vessel.
8.4 Remove the Bag Holder from the PTFE Insert.
8.5 Remove the filter bags from the Bag Holder.
8.6 Dry the samples for 15 –30 minutes in the oven at 102 ± 2°C or in the
ANKOMRD Dryer set at the factory setting of 110°C.
8.7 Remove the samples from the oven or dryer and place them in a Desiccant
Pouch.
8.8 Allow the samples to cool to room temperature. This should take about 10 –
15 minutes.
8.9 Re-weigh each filter bag (W3) immediately after removing from the Desiccant Pouch.
9.
Clean the fat from the bottom of the vessel using paper towel.
If too much fat accumulates in the bottom of the Extraction Vessel, an
overtemp fault will occur.