Boeco S-300 User manual

S-200/S-220/S-300
Spectrophotometer
INSTRUCTION MANUAL
Version V1.1

Instructions for installation and use
Appliance / Affair: Spectrophotometer
S-200/S-220/S-300 Spectrophotometer
Thank you for your purchase of S-200/S-220/S-300 spectrophotometer. In order to ensure proper use and
your safety, please read this manual carefully and keep it well before using the instrument.
Information contained in this manual is subject to change without notice for product appearance and technical
data. Enquiries are welcome.

Instructions for installation and use
Appliance / Affair: Spectrophotometer
IMPORTANT
Precautions on Electromagnetic Wave Interference
(1 )Possible Electromagnetic Wave Interference Caused by This Instrument
When this instrument is used in a residential area or an adjacent area thereto,
it may cause interference to radio and television reception.
To prevent this, use the specified system connection cables in strict
accordance with the instruction manual.
The instrument is designed to minimize possible electromagnetic wave
interference caused by it if the specified cables are connected properly.
However, there is no guarantee that electromagnetic wave interference will not
be caused by the instrument.
If the instrument does cause interference to radio or television reception, which
can be determined by turning off and on the instrument, the user is encouraged
to try to correct the interference by one or more of the following measures:
 Increase separation between the instrument and radio/TV receiver.
 Connect the instrument to an outlet on a circuit different from that to which
the radio/TV receiver is connected.
(2 )Possible Electromagnetic Wave Interference Affecting This Instrument
If this instrument is used near an intense electromagnetic source, interference
noise may be given to the instrument to incur an adverse effect on its
performance or functionality.
To prevent this, use the specified system connection cables in strict
accordance with the instruction manual.
The instrument is designed to minimize possible electromagnetic wave
interference affecting it if the specified cables are connected properly.
However, there is no guarantee that electromagnetic wave interference will not
occur in this instrument.
If the instrument does incur electromagnetic wave interference, which can be
determined by turning on and off possible sources of electromagnetic wave
interference nearby, the user is encouraged to try to correct the interference by
one or more of the following measures:
 Reorient the instrument.
 Increase separation between the instrument and possible sources of
electromagnetic wave interference.
 Increase separation between the power cable of the instrument and
possible sources of electromagnetic wave interference.
 Connect the instrument to an outlet on a circuit different from that to which
possible sources of electromagnetic wave interference are connected.
 Confirm that any other device connected with the instrument is not

Instructions for installation and use
Appliance / Affair: Spectrophotometer
affected by electromagnetic wave interference.
Warranty on Product
The Model S-200/S-220/S-300 spectrophotometer is warranted to operate
according to the specifications given in the instruction manual, provided it is
used in accordance with the instructions described in the manual.
(1) Scope of Warranty
(a) Any parts which prove to be defective in design or workmanship during
the warranty period will be repaired without charge.
(b) A substitute part may be used for repair, or replacement with an
equivalent product may be made instead of repair.
(c) Such system components as a personal computer and printer to be
updated frequently for improvement may not be available in original versions at
the time of replacement.
(2) Warranty Period
One year from the date of initial installation.
(3) Availability of Technical Support Service
Technical support service for this instrument is available within regular working
hours on workdays predetermined by us.
(4) Limitations and Exclusions on Warranty
Note that this warranty is void in the following cases, even if they occur within
the warranty period.
(a) Failure due to operation at a place not meeting the installation
requirements specified by us
(b) Failure due to power supply voltage/frequency other than specified by us
or due to abnormality in power supply
(c) Corrosion or deterioration of the tubing due to impurities contained in
reagent, gas, air or cooling water supplied by the user
(d) Corrosion of the electric circuits or deterioration of the optical elements
due to highly corrosive atmospheric gas
(e) Failure due to use of hardware, software or spare parts other than
supplied by us
(f) Failure due to improper handling or maintenance by the user
(g) Failure due to maintenance or repair by a service agent not approved or
authorized by us
(h) After disposal of this instrument, or after its resale without our approval
(i) Failure due to relocation or transport after initial installation
(j) Failure due to disassembly, modification or relocation not approved by us
(k) Consumables, and failure of parts that have reached the end of specified
useful life
(l) Failure of parts excluded from the warranty in the instruction manual or
other documents

Instructions for installation and use
Appliance / Affair: Spectrophotometer
(m) Failure due to acts of God, including fire, earthquake, storm, lightning,
social disturbance, riot, crime, insurrection, war (declared or undeclared),
radioactive pollution, contamination with harmful substances, etc.
(n) Failure of the hardware, or damage to the system software, application
software, data or hard disk due to computer virus infection
(o) Failure of the personal computer connected with the instrument, or
damage to the system software, application software, data or hard disk due to
power interruption or momentary power voltage drop caused by lightning or the
like
(p) Failure of the personal computer connected with the instrument, or
damage to the system software, application software, data or hard disk due to
disconnection of main power to the personal computer without taking the
specified normal shutdown procedure
(5) Disclaimer of Warranty
(a) Any express warranties other than the explicit conditions indicated in (1)
are excluded from this warranty.
(b) Any other implied warranties of merchantability and fitness for a particular
purpose are not included in this warranty. No liability is assumed for direct or
indirect damages arising out of explicit or implied warranties.
(c) Oral or written information or advice given by our dealers, distributors,
agents or employees without our express permission shall not create a
warranty or in any way increase the scope of this warranty.
Installation, Relocation and After-sale Technical Service
Installation of this instrument shall be carried out by or under supervision of
qualified service personnel of Boeckel + Co (GmbH + Co) KG or its authorized
service agent.
Before installation of the instrument, the user shall make preparations for
satisfying the installation requirements in accordance with the instruction
manual.
If relocation of the instrument becomes necessary after initial installation
(delivery), please notify your local sales representative or nearest service office
of Boeckel + Co (GmbH + Co) KG.
Disposal this instrument
When you discard equipment, please check and discard a related statute etc.
or ask the service section of Boeckel + Co (GmbH + Co) KG.
Other Precautions
(1) Handling of Chemicals and Samples
(a)The user is responsible for following relevant legal standards and
regulations in handling, storage and discarding of chemicals and samples used
in analytical operations of this instrument.
(b) Reagents, standard solutions and accuracy-control samples shall be
handled, stored and discarded as instructed by the respective suppliers.

Instructions for installation and use
Appliance / Affair: Spectrophotometer
(2) Notice on Instruction Manuals
(a)Information contained in the instruction manuals furnished with the
instrument is subjected to change without notice for product improvement.
(b) This manual is copyrighted by Boeckel + Co (GmbH + Co) KG with all rights
reserved.
(c) No part of this manual may be reproduced or transmitted in any form or by
any means without our express written permission.

Instructions for installation and use
Appliance / Affair: Spectrophotometer
SAFETY SUMMARY
CAUTION
For your safety please read the following precautions carefully before
using the instrument.
General Safety Guidelines
 For safe handling of this product, please follow the instruction procedure in
the manual for this product.
 Pay special attention to follow all the hazard warnings on the product and
in the manual. Failure to do so can cause injury to you or damage to the
product.
 After installation, please do not move the equipment. A vibration might
affect the adjustment of the product.
 The hazard warnings, which appear on the warning labels on the product
or in the manual, have one of the following alert headings consisting of an
alert symbol and a signal word, DANGER, WARNING, or CAUTION.
DANGER: Indicates an imminently hazardous situation that, if not
avoided, will result in death or serious injury. (It does not
apply to this equipment.)
WARNING: Indicates a potentially hazardous situation that, if not
avoided, can result in death or series injury.
CAUTION: Indicates a hazardous situation that, if not avoided, will or
can result in minor to moderate injury, or serious damage to
the product.
: The alert symbol shown precedes every signal word for hazard
warnings, and appears in safety related descriptions in the manual.
In addition, the following “Attention” and “Note” are not directly related to the
safety of a person:

Instructions for installation and use
Appliance / Affair: Spectrophotometer
△ Attention: It is used to present warnings, which are not directly related to
personal injury hazards. It is used to indicate prevention against damage to
the equipment.
◇ Note: This is used to indicate instructions that enable you to operate the
equipment accurately and perform accurate measurements.

Instructions for installation and use
Appliance / Affair: Spectrophotometer
SAFETY SUMMARY (Continued)
General Safety Guidelines (Continued)
Before using
 Before using this product, please make sure you read and understand the
instructions.
 Please keep this manual in a safe and easily accessible place so that you
can use it when necessary.
 Please make sure to use this product properly and follow the instructions
as specified in this manual.
 Please make sure to understand and follow the instructions regarding
safety in this manual.
 If you do not follow the instructions in this manual, an inaccurate analysis
may result or bodily injury may occur.
 Because of danger, please make sure not to modify or alter the product,
make sure not to use unspecified parts, and make sure not to operate the
equipment by removing/defeating the safety device(s).
 When using chemicals, please make sure to ventilate the room well. If
there is not enough ventilation, it may be hazardous to your health.
 Although we have carefully considered the instructions written on the
products and manuals, it is possible for an unexpected event to occur.
When operating the equipment, aside from following the instructions, be
very cautious.

Instructions for installation and use
Appliance / Affair: Spectrophotometer
SAFETY SUMMARY (Continued)
General Safety Guidelines (Continued)
Precautions for Installation • Maintenance • Relocation and After Sale Technical
Service
 Before installation, confirm that there are no missing items or standard
accessories. If there is something missing or damaged, or you have
noticed any problems, please contact our nearest representative.
 Operating the equipment without a standard part can damage the
equipment and cause safety concerns. If that occurs, please follow the
instruction of the installer.

Installation of this instrument shall be carried out by or under supervisions of qualified service
personnel of Boeckel + Co (GmbH + Co) KG or its aut
horized service agent.
 When relocation of this instrument becomes necessary after initial
installation (delivery), please notify your local Boeckel + Co (GmbH + Co)
KG sales representative or nearest Boeckel + Co (GmbH + Co) KG
service office. Technical support service for this instrument is available
from service agent approved or authorized by Boeckel + Co (GmbH + Co)
KG within regular working hours or workdays.
 Please do not perform any other operations that are not included in the
manual. If any problem occurs with the equipment, please contact the
agent from whom you purchased it or the service department of Boeckel +
Co (GmbH + Co) KG.

Instructions for installation and use
Appliance / Affair: Spectrophotometer
SAFETY SUMMARY (Continued)
WARNING: Poisoning from Organic Solvent Gas
Handling Organic Solvents
 The organic solvent vapor may be harmful to your health.
WARNING: Eye Injury from Organic Solvents
Handling Organic Solvents
 Please wear protective glasses when using organic solvents. If the organic
solvent should get into your eye, flush your eye immediately under running
water for at least 5 minutes while keeping your eyelids open. See a
physician for appropriate treatment.

Instructions for installation and use
Appliance / Affair: Spectrophotometer
SAFETY SUMMARY (Continued)
WARNING: Electrical Shocks from Improper Grounding
 When wiring the personal computer, power supply for the thermostatic cell
holder and the like, please make sure to use the 3-prong wire (with
ground) provided.
WARNING: Electrical Shock from Contact
 High voltage is used inside of the equipment, be sure to turn off the unit
before connecting the power cord.
CAUTION: Burns from high Temperature
 The lamp will become very hot during operation.
 Make sure that the instrument is switch off, the power cord is pulled off
and the D2 lamp and the tungsten lamp is cooled off when replacing the
lamp.
CAUTION: Fatigue due to Prolonged Work
 Viewing the display in your work can cause eye and physical fatigue if you
continue to work in the same posture for extended periods.
 When working with the display for a prolonged period, for your health,
make sure to take breaks for 10 to 15 minutes every hour in order to rest
your eyes and body.
CAUTION: Indoor ventilation
 If using UV zero detection system in a small room for a long time, it may
cause indoor nitrogen concentration increases and the oxygen
concentration drops, which will affect human health.
 Please install exhaust fan or open the windows frequently to maintain
good indoor ventilation.

Instructions for installation and use
Appliance / Affair: Spectrophotometer
SAFETY SUMMARY (Continued)
Electricity
(a) The voltage for the Spectrophotometer system and personal computer
must be a single-phase AC 100V to 240V; Variations in the voltage and
noise generated in the power line will cause adverse effects on the
spectrophotometer and may also cause accidents.
(b) Please make sure that grounding is provided together with the power
supply wires, and make sure that it is connected with a grounding
resistance of less than 4. Defective grounding may not only cause lower
resistance against noise from the outside but it can also cause the Mass
Spectrometer and personal computer to generate static electricity, which
may involve the danger of electrical shocks.
(c) A high voltage circuit is used inside the Spectrophotometer. Do not open
the covers when this circuit is operational because of the danger from
electrical shock.
Fire Extinguishers
(a) Do not smoke or use fire within 3 meters of the Spectrophotometer
(b) Make sure to keep a fire extinguisher near the Spectrophotometer at all
times. Obtain an ABC Powder extinguisher that can be used for normal fires,
oil fires, and electrical fires.

Instructions for installation and use
Appliance / Affair: Spectrophotometer
Functional characteristics
This series includes 2 models. S-200 is visible spectrophotometer, S-220/S-300 is UV-VIS
spectrophotometer.
Feature
Small and beautiful
appearance
4.3 inches, 480×272 resolution, TFT colorful liquid crystal touch screen
size: like A3 paper, 400(W)×280(D)×160(H) mm
weight: 4 kg
Easy operation One-button operation interface into the measurement function
High-speed wavelength move, arrive to any specified wavelength within 1 second
For S-200 spectrophotometer, when sampling interval is 0.2nm, its scanning
speed is 2400nm/min.
Unique application method manager changes the original function-oriented
operation method and user sample measurement method operation mode.
Energy saving and
environmental
protection
High conversion efficiency switching power supply, 100-240V AC voltage input,
and complete riddance of low grid voltage
S-200 spectrophotometer uses 2000 hours lifetime imported halogen lamp
S-220/S-300 spectrophotometer uses low-power, high brightness and 109 times
lifetime pulses xenon lamp
More ports Serial printer port for thermal printer
USB port for PC
SD card port saves data and measurement methods
Options port for connecting and controlling several options
More options Auto 5-cell holder
Auto sample sipper
Flow cuvette holder
10, 20, 30, 50, 100mm rectangular long-path cuvette holder
Micro-cuvette holder
Tube holder (only for S-200)
Electronic thermostat TC cuvette holder (only for S-220/S-300)

Instructions for installation and use
Appliance / Affair: Spectrophotometer
The instrument
Keypad
The instrument uses touch display screen, all operation can be by touch screen. Two shortcut key further
enhance easy operation.
BACK. Return to the previous menu.
MEASURE. Begin to a new measurement.
SD card slot

Instructions for installation and use
Appliance / Affair: Spectrophotometer
Operation environment
Power Voltage: AC 100V-240V, Frequency: 50/60Hz±1Hz, Capacity: more than 200W
Grounding line resistance of 10Ω or less is required
The input power mutations, without interference from other large electrical equipment
Operating
temperature 5-35℃
In order to perform a measurement under the most stable condition, we recommend
that the instrument is used in an air conditioned room of 20-25℃.
Storage temperature:-20-55℃
Operating
humidity
Less than 85%.
We recommend that the instrument is used under 30%～70% humidity.
Storage humidity: less than 85%。
Workbench Width: more than 550mm, Depth: more than 400mm, Load bearing: more than 15kg,
horizontal and reliable workbench.
If there are other auxiliary devices, it is necessary to enlarge workbench area.
More than 200mm space at the both sides of the instrument, avoid being close to the
wall.
Height of the instrument: about 160mm. In order to easy operation, please choose
suitable workbench.
Connect with
other equipment
Connect with PC (additional optional software and computer). Indirect specify type
thermal printer (options) and other accessories.
When main unit connects with above equipments, please switch off the power of the
main unit and other equipments.
All grounding cords are in good condition and can be connected with the grounding
line of the main unit.

Instructions for installation and use
Appliance / Affair: Spectrophotometer
Specification ( )Ⅰ
S-200
Wavelength range 320-1100nm
Wavelength controlled
variable
0.2nm
Wavelength accuracy ±1nm
Wavelength repeatability ≤0.5nm
Transmittance accuracy ±0.5％T (NIST 930 Filter)
Transmittance repeatability
0.2％T
Baseline flatness ±0.002Abs (330-1090nm)
Noise level ≤0.001Abs (500nm )
Baseline stability ≤0.001Abs/h (500nm, 2 hours after warm up )
Spectral bandwidth 6nm±1.2nm
Stray light ≤0.5％T
Wavelength scan speed 2400nm/min (0.2nm sampling interval, without filter
switchover)
Wavelength move speed To any specified position within 1 second
Light source WI lamp
Detector silicon photodiode
Display screen 4.3 inches, 480×272 colorful touch LCD screen
Printer specified 80-column thermal printer (series port)
Metering mode Single beam
Dimension (W×D×H) 400×280×160mm
Weight About 4 kg
Power requirement AC, 100-240V, (50/60Hz)
Power consumption 100VA
Communication ports Serial printer port connects thermal printer
USB port connects PC
SD card port saves data and measurement methods
Accessories port connects and controls several options
Optional Accessories Auto 5-cell holder
Auto sample sipper
Flow cuvette holder
10, 20, 30, 50, 100mm long-path cuvette holder
Micro-cuvette holder
Tube holder

Instructions for installation and use
Appliance / Affair: Spectrophotometer
Specification ( )Ⅱ
S-220/S-300
Wavelength range 190-1000nm
Wavelength controlled
variable
0.2nm
Wavelength accuracy ±2nm
Wavelength repeatability ≤1nm
Transmittance accuracy ±1％T (NIST 930 Filter)
Transmittance repeatability
0.5％T
Baseline flatness ±0.005Abs (200-990nm)
Noise level ≤0.005Abs (250nm)
Baseline stability ≤0.005Abs/h (250nm, after 2 hours warm up)
Spectral bandwidth 5nm±1nm
Stray light ≤0.5％T
Wavelength scan speed 300nm/min (0.2nm sampling interval, without filter
switchover)
Wavelength move speed To any specified position within 1 second
Light source pulsed-xenon lamp
Detector silicon photodiode
Display screen 4.3 inches, 480×272 colorful touch LCD screen
Printer specified 80-column thermal printer (series port)
Metering mode Single beam
Dimension (W×D×H) 400×280×160mm
Weight About 4 kg
Power requirement AC, 100-240V, (50/60Hz)
Power consumption 100VA
Communication ports Serial printer port connects thermal printer
USB port connects PC
SD card port saves data and measurement methods
Accessories port connects and controls several options
Optional Accessories Auto 5-cell holder
Auto sample sipper
Flow cuvette holder
10, 20, 30, 50, 100mm long-path cuvette holder
Micro-cuvette holder
Electronic thermostat TC cuvette holder

Instructions for installation and use
Appliance / Affair: Spectrophotometer
The operation interface
Shortcut appear on every interface and can quickly switch to different measurement functions.
Measurement appear on every measure interface for basic measurement and setting
Data Process appear on scan interface. For the results of the scan data processing and according to
different scanning characteristic, automatically vary corresponding processing ways.
Graph display buttons appear only in scan interface for graphical display changes
Status bar show in every interface to display current time, wavelength and readings in transmission or
absorbance
Clicking the wavelength in the Status bar, it will go to the setting screen for wavelength setup.
Clicking the reading in the Status bar, it will go to the large display measurement interface to achieve simple
transmittance and absorbance read function.
The value in the status bar of S-200 is real-time reading value of current wavelength.
The value in the status bar of S-220/S-300 is the last time measured value.
Status bar Shortcut
Measurement Data process

Instructions for installation and use
Appliance / Affair: Spectrophotometer
Word input keyboard
This keyboard appears when there is a need for word input, for example, sample name, user and document
name.
Close the keyboard
Clear
Switching lowercase, digit and sign
Enter
Numeric input
Numeric input appears at the area where can input digit, for example, wavelength value and scanning time.