Byonoy Absorbance One User manual
Manufacturer:
Byonoy GmbH
Bernstorffstr. 118
22767 Hamburg, Germany
00 49 40 5379 866 00
service@byonoy.com
www.byonoy.com
Instructions for Use
Absorbance One
Valid for REF Number ABS CU A 01
App Version 1.2.0
Instructions for Use
Absorbance One
Version 1.1 (05/08/2021)
©Copyright 2021 by Byonoy GmbH. All rights reserved.
II
Dear customer,
We are delighted that you have chosen the Absorbance One. To take full advantage of the
instrument's performance and to enjoy your instrument for many years, please read these
instructions for use carefully before installation and commissioning. Operate the instrument
in accordance with these instructions. The operating-safety and function of the instrument
can only be guaranteed if both the general safety regulations and accident prevention reg-
ulations of the legislator as well as the safety instructions in this manual are observed. We
accept no liability for any damage resulting from improper use or incorrect operation.
Ensure that the manual is always accessible and is read and understood by all
persons operating the instrument.
This user manual may only be used according to its intended purpose. It may not be repro-
duced, changed, or translated into another language without the prior written consent of
Byonoy GmbH.
This document is subject to technical changes and updates.
III
TABLE OF CONTENTS
1
General information.......................................................................................................................1
2
Overview of the Absorbance One...................................................................................... 3
2.1 Scope of application..................................................................................................................................3
2.2 Measurement method..............................................................................................................................3
2.3 Absorbance One System .......................................................................................................................3
3
Preparing the product for use.............................................................................................. 6
3.1 Unpacking, storage, and transport.................................................................................................6
3.2 Supplied materials.......................................................................................................................................6
3.3 Working environment...............................................................................................................................6
3.4 App installation procedure...................................................................................................................7
4
Operation........................................................................................................................................... 8
4.1 Computer connection...............................................................................................................................8
4.2 Initialisation.......................................................................................................................................................8
4.3 Perform a measurement .........................................................................................................................8
4.4 Results..................................................................................................................................................................9
5
Signals and troubleshooting.................................................................................................10
5.1 Signal lights ....................................................................................................................................................10
5.2 Troubleshooting.........................................................................................................................................10
6
Maintenance and cleaning.....................................................................................................12
6.1 Maintenance..................................................................................................................................................12
6.2 Cleaning ............................................................................................................................................................12
6.3 Technical support .....................................................................................................................................13
6.4 Repairs ...............................................................................................................................................................13
7
Technical specifications..........................................................................................................14
8
Guarantee.........................................................................................................................................15
9
Declaration of conformity.......................................................................................................16
General information
1
1
General information
Important: Follow the instructions for use
Any commissioning or handling of the instrument requires precise knowledge and notice of
this manual. The device is intended for the described use only.
In this manual, particularly important remarks are labeled as followed:
Warning:
This is a warning that indicates an immediately or potentially dangerous situa-
tion, which, if not avoided, will result in death or serious injury.
Caution:
Caution indicates a potentially hazardous situation, which, if not avoided, could
result in minor or moderate injury.
Information:
This is a piece of information indicating certain properties that must be ob-
served.
Liability for function and damage
Liability for the function of the device shall, in any case, pass to the owner or operator if the
device is improperly maintained, repaired, or modified by persons who do not belong to the
authorized service personnel, or if it is handled in a way that does not comply with its in-
tended use. The service and operation of the product must be in accordance with this man-
ual. Byonoy shall not be liable for damages resulting from non-observance of the information
above. Warranty and liability conditions of the terms of sale and delivery of Byonoy are not
extended by the information above.
Information
An incorrect working environment can lead to a reduction in service life, dam-
age to the device, or measurement errors.
To avoid measurement errors, it is essential to ensure that the instrument is
connected properly and that the experiments are carried out correctly.
Incorrect cleaning of the instrument can reduce its service life and can cause
damage to the instrument.
Follow all safety instructions on the device and in the attached documents.
Follow all general precautions that apply to electrical instruments.
General information
2
Disposal of used electrical and electronic equipment
The symbol on the product or its packaging indicates that this product is not to be treated
as normal household waste. It must be disposed of at a collection point for the recycling of
electrical and electronic equipment. By contributing to the correct disposal of this product,
you protect the environment and the health of your fellow human beings. Recycling helps to
reduce the consumption of raw materials. For further information on how to recycle this
product, please contact your local authority or municipal waste disposal centres.
Working with biological and harmful material
The Absorbance One is
not
to be used for the measurement of biohazardous substances.
Always observe the manufacturer's hazard information pertaining to the substances to be
measured.
The instrument does not produce any toxic or harmful gases or substances. During the
measurement, make sure that there are no toxic or harmful substances in the cuvette.
Overview of the Absorbance One
3
2
Overview of the Absorbance One
2.1
Scope of application
The Absorbance One is an optical laboratory instrument for measuring the absorbance (op-
tical density) of biological or non-biological samples in cuvettes, according to the specifica-
tions described in this user manual. The Absorbance One is intended for research and other
non-in-vitro-diagnostic analyses only. It is to be operated by trained laboratory personnel
and is intended for professional use.
2.2
Measurement method
The Absorbance One is designed to carry out sensitive absorbance measurements. It
measures the optical density (OD) of samples at defined wavelengths.
Absorption
Absorption refers to the amount of light absorbed by a medium. Absorption reduces trans-
mission. Transmission is the ratio of incident to transmitted light. Accordingly, the degree of
transmission is calculated as follows
T = (I/I0)
where I is transmitted light and I0is incident light.
Optical Density
Optical density is a measure of the attenuation of light radiation after it has passed through
a medium. Optical density is the logarithmic quantity that describes the reciprocal of the
transmittance T:
OD = log(I0/I)
where I is the transmitted light and I0is incident light.
Optical density is the absorbance of the sample plus other attenuating effects such as scat-
tered light due to turbidity. It is therefore necessary to avoid scattered light in order to meas-
ure absorbance correctly.
If no other attenuating effects are present, absorbance = optical density.
2.3
Absorbance One System
Absorbance One Reader
The Absorbance One is an absorbance-based measuring device, i. e. a measuring instrument,
with which the absorbance values of a cuvette can be read, recorded and provided for fur-
ther processing. The Absorbance One is a solid-state, single-beam, single-wavelength cu-
vette photometer.
Overview of the Absorbance One
4
The cuvette is inserted manually into the slot from top of the device. An LED is used as a
status indicator for the device.
On the back of the Absorbance One there is a USB-C port for connecting the instrument to
a computer via the included USB-C cable.
Power consumption
The Absorbance One is an analytical instrument with very low power consumption. It is pow-
ered by a USB-C cable and the total power consumption is generally less than 2.5 watts.
Absorbance One App
The Absorbance One App is used to operate the Absorbance One Reader and process the
results. After the App is started and the reader is connected, the main window appears au-
tomatically.
Figure 1.
Overview of the Absorbance One App.
The Absorbance One App displays every measurement as a separate card. Multiple meas-
urements are then correspondingly arranged in a stack. Two buttons are arranged in the
upper left corner, and a series of status indicators are displayed on the right.
Overview of the Absorbance One
5
Consumables
With the Absorbance One, Macro, Semi-micro and Micro cuvettes can be used.
Figure 2.
Type of cuvettes, which can be used with the Absorbance One.
When using cuvettes, always check the specifications of the cuvette manufacturer. Not all
cuvettes of a particular manufacturer are the same in design, materials, or configuration.
Preparing the product for use
6
3
Preparing the product for use
3.1
Unpacking, storage, and transport
Unpacking
Remove the packaging material and carefully place the device on a firm and level surface.
Check the device for external damage and check the materials supplied (see chapter 3.2).
The device is packed in a specially designed cardboard box. Keep the packaging material. If
the device must be returned for repair, the original packaging material must be used.
Storage and protection during the intervals of normal use
Protect the device from dust during prolonged storage.
When storing the device, ensure that the temperature and humidity are within
the specified range (see chapter 7).
Transportation
Before transportation, unplug the instrument and ensure there is no cuvette inside the de-
vice. Depending on the transport distance, use the original packaging material. Make sure
that the new location meets the requirements described in chapter 3.3.
For transport, ensure the product is well-packaged to avoid damage in transit.
3.2
Supplied materials
When unpacking the device, please check that the following components are present:
• Absorbance One Reader
• USB-C cable
• USB stick
3.3
Working environment
Due to its small size, the Absorbance One can be operated very well in various working en-
vironments.
However, the following information should be observed. Ignoring it may lead to measurement
errors and a reduction in the expected lifetime of, or damage to, the device:
The device should stand on a level surface, free from dust and vibrations.
Do not operate the device near heat sources or under direct sunlight.
Preparing the product for use
7
When operating the device, ensure that the temperature and humidity are
within the specified range (see chapter 7).
Place the instrument in a horizontal position secured against falling.
3.4
App installation procedure
The App is compatible with Windows 10.
To install the Absorbance One App, insert the USB stick into the computer and follow the
steps below:
• Locate and select the USB drive in the document browser.
• Open the installation file.
• Follow the steps in the Setup Wizard to complete the installation.
To install the App on the computer, the user must have administrator rights or
obtain such permissions.
Uninstalling the software
The Absorbance One App can be uninstalled using the computer’s standard uninstall proce-
dure.
Operation
8
4
Operation
Before commissioning the Absorbance One, you should carefully read and understand the
entire manual to familiarize yourself with the system.
4.1
Computer connection
The Absorbance One requires a connection to the computer for power supply, starting an
experiment, and data transfer. Insert the USB-C cable into the Absorbance One and the USB
port of the computer. The Absorbance One starts automatically when connected to a com-
puter via the USB-C cable and the software automatically establishes a connection.
Always use the included USB-C cable for the connection to your computer.
Connect the instrument directly to your computer and do not use an external
USB hub.
The device may only be operated at a USB port USB 2/USB 3 with 5 VDC and a
maximum of 3 A of a certificated computer (with certification mark of an ap-
proved testing laboratory). Use an adapter cable on USB 3.1 with type C plug
When the Absorbance One is switched on, the LED status light on the top of the instrument
will illuminate. Each time the instrument is switched on, an internal self-test is performed to
ensure there are no malfunctions.
4.2
Initialisation
An initialization occurs automatically after connection of the device to the computer and is
repeated at regular intervals thereafter. The initialization determines the zero point, which
is applied for the next measurement. The initialization can only be performed when the slot
is empty, and no cuvette is inside the device.
4.3
Perform a measurement
The Absorbance One has an auto-measurement function. Upon insertion of a cuvette, meas-
urement is triggered automatically, and the result is immediately displayed in the App.
During measurement, the status LED will display an amber colour. After measurement, the
status LED will turn green and the result of the measurement will be displayed as a white
card in the App.
In order to guarantee a correct measurement result, please pay attention to the following
information:
When inserting the cuvette, pay attention to its alignment, as cuvettes have a
designed direction.
Do not touch the device or the cuvette during measurement.
Operation
9
The side walls of the cuvette must be dry before it is inserted into the instru-
ment.
Insert the cuvette quickly and release it during measurement.
4.4
Results
After a successful measurement, the results will be automatically shown in the App.
Figure 3.
Sample card.
By clicking on the card the following functions are available:
• The result can be designated as a Sample or a Blank.
• In the Sample Card, an ID can be assigned.
• The result can be deleted.
• The measurement can be repeated.
If a blank is defined, all the other cards are automatically blank corrected.
Via the “Export” button, the results can be exported as a PDF report or CSV file.
The “New” button will delete all measurement cards and results.
Signals and troubleshooting
10
5
Signals and troubleshooting
5.1
Signal lights
Table 1.
Explanation of the signal lights
Activity of the signal light
Meaning
White, blinking
Self-test is being performed
White, continuous
Ready for measurement
Amber
Measuring
Green
Measurement complete
Red
Hardware error
5.2
Troubleshooting
Failed auto initialisation
The Absorbance One features an auto initialisation. In rare cases, the auto initialisation fails.
This can be recognized in the App, as the initialization will not be confirmed, and on the de-
vice itself, where the status LED will show green although no cuvette is in the device. In this
case, the initialization must be triggered manually. Ensure the slot is empty and no cuvette is
inside, then click the repeat button next to the initialization status indicator in the upper right
corner of the software. The status LED will turn white, whereupon the device is ready for the
measurement.
Figure 4.
Failed auto initialisation.
Signals and troubleshooting
11
Error codes
In the case of an issue with the device, error codes will appear in the software.
Table 2.
Description of error codes
Type of Error
Cause
Solution
Ambient light error
Too much ambient light is reach-
ing the device, or the device is de-
fective.
Reduce the amount of ambi-
ent light present.
Insufficient light er-
ror
The device is dirty, the slot is oc-
cupied, or the device is defective.
Please clean the slot and re-
move obstructions.
Unrecoverable er-
ror, Hardware error
Critical error.
Contact customer support.*
Timeout error
The measurement was disrupted
by a shadow in the device.
Repeat the measurement. En-
sure the cuvette is not dis-
turbed during the measure-
ment and no shadow is cast
on the slot.
Noise limit error
USB cable/USB hub problem or
defective device.
Please contact customer sup-
port.
Device communi-
cation error
Communication with device not
possible.
Please reconnect device.
Device is discon-
nected
Error communicating with the de-
vice.
Please reconnect device.
*Please send the logfile to customer support. The logfile can be found under
“C:\Users\(username)\AppData\Local\Vir-
tualStore\Program Files\(x86)\Byonoy\Absorbance One App\app”.
Alternatively, you can search “AbsorbanceOne-
App.exe.log” in your file browser.
Maintenance and cleaning
12
6
Maintenance and cleaning
6.1
Maintenance
The Absorbance One is maintenance-free. Each time the instrument is switched on, an in-
ternal self-test is carried out to ensure there are no malfunctions.
There are no parts within the Absorbance One that can be serviced by the customer. It is
only necessary to ensure that the device is kept clean.
The accuracy, linearity and reproducibility of the device can be checked by the manufac-
turer. Please contact the manufacturer.
6.2
Cleaning
Before cleaning, detach USB-C cable from the instrument and remove the cuvette.
Cleaning the housing
The surfaces of the housing should be cleaned regularly. This can be done using a moistened
cloth or similar textile. Do not soak the cloth. Wipe away any residual moisture from the sur-
face of the device. Do not use scouring agents.
Cleaning the slot and the optical components
To clean the slot of the Absorbance One, use cotton swaps. If needed, the cotton swaps can
be slightly soaked with 80% ethanol to carefully remove dust or contamination. Carefully
clean the center of the filter and lens, which are in the optical path. See Figure 4 for a detailed
view of the filter and lens position.
Figure 5.
Cross section of the reader with inserted cuvette. 1 LED, 2 Filter, 3 Cuvette, 4 Ball
lens, 5 Photodiode, 6 PCB Board.
Maintenance and cleaning
13
Information and warnings for cleaning
When cleaning, pay attention to the following information. Failure to observe this information
may result in damage to the instrument. This can lead to a reduction of the service life or
measurement errors:
Always avoid spraying liquid directly onto the surfaces of the Absorbance One.
This is especially important for the inside of the slot, where there are optical el-
ements that are very sensitive and essential for the functioning of the instru-
ment.
Never clean the inside of the slot with sharp or abrasive scourges, and do not
use aggressive solvents or corrosive agents.
Biological hazard: Always wear gloves during cleaning operations that may in-
volve contact with biological or generally hazardous materials or liquids.
6.3
Technical support
In case of problems with the instrument, please contact the manufacturer’s service depart-
ment or your local representative.
Biological hazard: It is your responsibility to decontaminate the instrument and
all accessories before servicing and before returning the instrument or acces-
sories to the manufacturer.
For decontamination of the Absorbance One, follow the government guidelines for inactiva-
tion of organisms used in biological laboratories.
6.4
Repairs
Repairs on the device may only be carried out by the manufacturer. Please contact the ser-
vice department. The product warranty is voided if the device is modified by unauthorized
persons, or different parts are installed.
Technical specifications
14
7
Technical specifications
Table 3.
Performance and technical data
Parameter
Value
Type of product
Single beam, single wavelength cuvette pho-
tometer
Product name
Absorbance One
Housing material
Aluminium
Temperature (storage/operation)
5–50 °C
Relative tolerated humidity
(storage/operation)
Max. 70 %
Measurement method
Absorbance
Cuvette types
Macro, Semi-micro and Micro cuvettes
Light source
LED
Wavelength range
340-1000 nm
Detection
Photodiode
Measurement range
0–5.0 OD
Resolution
0.001 OD
Accuracy*
≤0,5 % + 0.005 OD from 0.0–3.0 OD
Reproducibility**
≤0.5 % + 0.005 OD from 0.0–3.0 OD
Linearity***
≤0,5 % from 0,0–3.0 OD
Connection to computer
USB 2/USB 3 with 5 VDC and max. 3 A
Dimensions
33 mm x Ø 64 mm
Power supply
5 VDC
Nominal value/Characteristic of the fuse
1 A/very fast-acting
Power input
2.5 W
Weight
150 g
* Accuracy is the maximum deviation between the determined value and the true value.
** Reproducibility is the maximum deviation between the determined values when the measurement
is repeated directly.
*** Linearity is the maximum deviation between the true and the determined increase of the value.
Guarantee
15
8
Guarantee
The standard warranty period of the Absorbance One is 24 months. If a defect manifests
itself in your device during the warranty period, please contact the service department di-
rectly.
The instrument may only be operated in technically perfect condition. In the event of defects
that could endanger employees or third parties, the device may only be used again after it
has been repaired by the manufacturer.
This warranty does not cover damage caused by improper use or external mechanical influ-
ences, transport damage, or unauthorized intervention in the device by unauthorized per-
sons.
Declaration of conformity
16
9
Declaration of conformity
This manual suits for next models
1
Table of contents
