Byonoy Absorbance One User manual

Manufacturer:
Byonoy GmbH
Bernstorffstr. 118
22767 Hamburg, Germany
00 49 40 5379 866 00
[e-mail]
www.byonoy.com
Instructions for Use
Absorbance One
Valid for REF Number ABS CU A 01
App Version 1.2.0
Instructions for Use
Absorbance One
Version 1.1 (05/08/2021)
©Copyright 2021 by Byonoy GmbH. All rights reserved.

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Dear customer,
We are delighted that you have chosen the Absorbance One. To take full advantage of the
instrument's performance and to enjoy your instrument for many years, please read these
instructions for use carefully before installation and commissioning. Operate the instrument
in accordance with these instructions. The operating-safety and function of the instrument
can only be guaranteed if both the general safety regulations and accident prevention reg-
ulations of the legislator as well as the safety instructions in this manual are observed. We
accept no liability for any damage resulting from improper use or incorrect operation.
Ensure that the manual is always accessible and is read and understood by all
persons operating the instrument.
This user manual may only be used according to its intended purpose. It may not be repro-
duced, changed, or translated into another language without the prior written consent of
Byonoy GmbH.
This document is subject to technical changes and updates.

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TABLE OF CONTENTS
1 General information.......................................................................................................................1
2 Overview of the Absorbance One...................................................................................... 3
2.1 Scope of application..................................................................................................................................3
2.2 Measurement method..............................................................................................................................3
2.3 Absorbance One System .......................................................................................................................3
3 Preparing the product for use.............................................................................................. 6
3.1 Unpacking, storage, and transport.................................................................................................6
3.2 Supplied materials.......................................................................................................................................6
3.3 Working environment...............................................................................................................................6
3.4 App installation procedure...................................................................................................................7
4 Operation........................................................................................................................................... 8
4.1 Computer connection...............................................................................................................................8
4.2 Initialisation.......................................................................................................................................................8
4.3 Perform a measurement .........................................................................................................................8
4.4 Results..................................................................................................................................................................9
5 Signals and troubleshooting.................................................................................................10
5.1 Signal lights ....................................................................................................................................................10
5.2 Troubleshooting.........................................................................................................................................10
6 Maintenance and cleaning.....................................................................................................12
6.1 Maintenance..................................................................................................................................................12
6.2 Cleaning ............................................................................................................................................................12
6.3 Technical support .....................................................................................................................................13
6.4 Repairs ...............................................................................................................................................................13
7 Technical specifications..........................................................................................................14
8 Guarantee.........................................................................................................................................15
9 Declaration of conformity.......................................................................................................16

General information
1
1 General information
Important: Follow the instructions for use
Any commissioning or handling of the instrument requires precise knowledge and notice of
this manual. The device is intended for the described use only.
In this manual, particularly important remarks are labeled as followed:
Warning:
This is a warning that indicates an immediately or potentially dangerous situa-
tion, which, if not avoided, will result in death or serious injury.
Caution:
Caution indicates a potentially hazardous situation, which, if not avoided, could
result in minor or moderate injury.
Information:
This is a piece of information indicating certain properties that must be ob-
served.
Liability for function and damage
Liability for the function of the device shall, in any case, pass to the owner or operator if the
device is improperly maintained, repaired, or modified by persons who do not belong to the
authorized service personnel, or if it is handled in a way that does not comply with its in-
tended use. The service and operation of the product must be in accordance with this man-
ual. Byonoy shall not be liable for damages resulting from non-observance of the information
above. Warranty and liability conditions of the terms of sale and delivery of Byonoy are not
extended by the information above.
Information
An incorrect working environment can lead to a reduction in service life, dam-
age to the device, or measurement errors.
To avoid measurement errors, it is essential to ensure that the instrument is
connected properly and that the experiments are carried out correctly.
Incorrect cleaning of the instrument can reduce its service life and can cause
damage to the instrument.
Follow all safety instructions on the device and in the attached documents.
Follow all general precautions that apply to electrical instruments.

General information
2
Disposal of used electrical and electronic equipment
The symbol on the product or its packaging indicates that this product is not to be treated
as normal household waste. It must be disposed of at a collection point for the recycling of
electrical and electronic equipment. By contributing to the correct disposal of this product,
you protect the environment and the health of your fellow human beings. Recycling helps to
reduce the consumption of raw materials. For further information on how to recycle this
product, please contact your local authority or municipal waste disposal centres.
Working with biological and harmful material
The Absorbance One is
not
to be used for the measurement of biohazardous substances.
Always observe the manufacturer's hazard information pertaining to the substances to be
measured.
The instrument does not produce any toxic or harmful gases or substances. During the
measurement, make sure that there are no toxic or harmful substances in the cuvette.

Overview of the Absorbance One
3
2 Overview of the Absorbance One
2.1 Scope of application
The Absorbance One is an optical laboratory instrument for measuring the absorbance (op-
tical density) of biological or non-biological samples in cuvettes, according to the specifica-
tions described in this user manual. The Absorbance One is intended for research and other
non-in-vitro-diagnostic analyses only. It is to be operated by trained laboratory personnel
and is intended for professional use.
2.2 Measurement method
The Absorbance One is designed to carry out sensitive absorbance measurements. It
measures the optical density (OD) of samples at defined wavelengths.
Absorption
Absorption refers to the amount of light absorbed by a medium. Absorption reduces trans-
mission. Transmission is the ratio of incident to transmitted light. Accordingly, the degree of
transmission is calculated as follows
T = (I/I0)
where I is transmitted light and I0is incident light.
Optical Density
Optical density is a measure of the attenuation of light radiation after it has passed through
a medium. Optical density is the logarithmic quantity that describes the reciprocal of the
transmittance T:
OD = log(I0/I)
where I is the transmitted light and I0is incident light.
Optical density is the absorbance of the sample plus other attenuating effects such as scat-
tered light due to turbidity. It is therefore necessary to avoid scattered light in order to meas-
ure absorbance correctly.
If no other attenuating effects are present, absorbance = optical density.
2.3 Absorbance One System
Absorbance One Reader
The Absorbance One is an absorbance-based measuring device, i. e. a measuring instrument,
with which the absorbance values of a cuvette can be read, recorded and provided for fur-
ther processing. The Absorbance One is a solid-state, single-beam, single-wavelength cu-
vette photometer.

Overview of the Absorbance One
4
The cuvette is inserted manually into the slot from top of the device. An LED is used as a
status indicator for the device.
On the back of the Absorbance One there is a USB-C port for connecting the instrument to
a computer via the included USB-C cable.
Power consumption
The Absorbance One is an analytical instrument with very low power consumption. It is pow-
ered by a USB-C cable and the total power consumption is generally less than 2.5 watts.
Absorbance One App
The Absorbance One App is used to operate the Absorbance One Reader and process the
results. After the App is started and the reader is connected, the main window appears au-
tomatically.
Figure 1.
Overview of the Absorbance One App.
The Absorbance One App displays every measurement as a separate card. Multiple meas-
urements are then correspondingly arranged in a stack. Two buttons are arranged in the
upper left corner, and a series of status indicators are displayed on the right.

Overview of the Absorbance One
5
Consumables
With the Absorbance One, Macro, Semi-micro and Micro cuvettes can be used.
Figure 2.
Type of cuvettes, which can be used with the Absorbance One.
When using cuvettes, always check the specifications of the cuvette manufacturer. Not all
cuvettes of a particular manufacturer are the same in design, materials, or configuration.

Preparing the product for use
6
3 Preparing the product for use
3.1 Unpacking, storage, and transport
Unpacking
Remove the packaging material and carefully place the device on a firm and level surface.
Check the device for external damage and check the materials supplied (see chapter 3.2).
The device is packed in a specially designed cardboard box. Keep the packaging material. If
the device must be returned for repair, the original packaging material must be used.
Storage and protection during the intervals of normal use
Protect the device from dust during prolonged storage.
When storing the device, ensure that the temperature and humidity are within
the specified range (see chapter 7).
Transportation
Before transportation, unplug the instrument and ensure there is no cuvette inside the de-
vice. Depending on the transport distance, use the original packaging material. Make sure
that the new location meets the requirements described in chapter 3.3.
For transport, ensure the product is well-packaged to avoid damage in transit.
3.2 Supplied materials
When unpacking the device, please check that the following components are present:
• Absorbance One Reader
• USB-C cable
• USB stick
3.3 Working environment
Due to its small size, the Absorbance One can be operated very well in various working en-
vironments.
However, the following information should be observed. Ignoring it may lead to measurement
errors and a reduction in the expected lifetime of, or damage to, the device:
The device should stand on a level surface, free from dust and vibrations.
Do not operate the device near heat sources or under direct sunlight.

Preparing the product for use
7
When operating the device, ensure that the temperature and humidity are
within the specified range (see chapter 7).
Place the instrument in a horizontal position secured against falling.
3.4 App installation procedure
The App is compatible with Windows 10.
To install the Absorbance One App, insert the USB stick into the computer and follow the
steps below:
• Locate and select the USB drive in the document browser.
• Open the installation file.
• Follow the steps in the Setup Wizard to complete the installation.
To install the App on the computer, the user must have administrator rights or
obtain such permissions.
Uninstalling the software
The Absorbance One App can be uninstalled using the computer’s standard uninstall proce-
dure.