ChromTech UV-3000 User manual

UV-3 SERIES SPECTROPHOTOMETER
Contents
SAFETY ………………………………………………………………….……… 1
General ………………………………………………………………………. 1
Electrical …………………………………………………………………….. 1
Warning ………………………………………………………………………… 1
Working Principle ………………………………………………………… 2
Unpacking Instructions …………………………………………………… 2
Specifications ……………………………………………..……………… 3
Installation …………………………………………………………………… 3
Operation ………………………………………………………..……………… 4
Prepare the Spectrophotometer ………………………………………… 4
Description of keys ………… …………………………………… 5
Turn on spectrophotometer ……………………………………………… 6
Basic operation…………………………………………………………… 7
Analyse Sample …………………………………………………………… 12
Basic Mode …………………………………………………………….. 12
Quantitative ….……………………………………………………….. 14
WL Scan ………………………………………………………………... 20
Kinetics …….………………………………………………………………… 25
DNA/Protein ……………………………………………………………… 28
Multi Wavelength ………………………………………………………….. 31
Utility ……………………………………………………………………… 33
Appendix A ………………………………………………….………… 41
Appendix B ………………………………………………….………… 42
Appendix C ………………………………………………….………… 44
Lamp replacement………………………………………………….…… 44
Replace W lamp………………………………………………….……44
Replace D2 lamp………………………………………………….… 45
Battery replacement………………………………………………….…… 46
Trouble shooting………………………………………………….………… 47

UV-3 SERIES SPECTROPHOTOMETER
Safety:
The safety statements in this manual comply with the requirements of the
HEALTH AND SAFETY AT WORK ACT, 1974.
Read the following before installing and using the instrument and its
accessories. The ChromTech UV-3 SERIES should be operated by
appropriate laboratory technicians.
General:
The apparatus described in this manual is designed to be used by properly
trained personnel in a suitable equipped laboratory. For the correct and safe
use of this apparatus it is essential that laboratory personnel follow generally
accepted safe procedures in addition to the safety precautions called for in this
manual.
The covers on this instrument may be removed for servicing. However, the
inside of the power supply unit is a hazardous area and its cover should not be
removed under any circumstances. There are no serviceable components
inside this power supply unit. For ChromTech UV-3 SERIES, avoid touching
the high voltage power supply at all times.
Some of the chemicals used in spectrophotometry are corrosive and/or
inflammable and samples may be radioactive, toxic, or potentially infective.
Care should be taken to follow the normal laboratory procedures for handling
chemicals and samples.
Electrical:
Before switching on the apparatus, make sure it is set to the voltage of the
local power supply (see Fig.1-1).
Fig.1-1
Voltage Switch
The power cord shall be inserted in a socket provided with a protective earth
contact. The protective action must not be negated by the use of an
extension cord without a protective conductor.
Warning:
Any interruption of the protective conductor inside or outside the apparatus or
disconnection of the protective earth terminal is likely to make the apparatus
dangerous. Intentional interruption is prohibited.
2

UV-3 SERIES SPECTROPHOTOMETER
Whenever it is likely that the protection has been impaired, the apparatus shall
be made inoperative and be secured against any unintended operation.
NEVER touch or handle the power supply on ChromTech UV-3 SERIES due to
the high voltage.
The protection is likely to be impaired if, for example, the apparatus
 Shows visible damage
 Fails to perform the intended measurements
 Has been subjected to prolonged storage under unfavorable conditions
 Has been subjected to severe transport stresses
WorkingPrinciple:
The spectrophotometer consists of five parts: 1) Halogen or deuterium lamps
to supply the light; 2) A Monochromator to isolate the wavelength of interest
and eliminate the unwanted second order radiation; 3) A sample compartment
to accommodate the sample solution; 4) A detector to receive the transmitted
light and convert it to an electrical signal; and 5) A digital display to indicate
absorbance or transmittance. The block diagram (Fig 1) below illustrates the
relationship between these parts.
Block diagram for the Spectrophotometer
100%T
0
Light Mono- Sample Detector Display
Source chromator Compartment
Fig1-2
In your spectrophotometer, light from the lamp is focused on the entrance slit
of the monochromator where the collimating mirror directs the beam onto the
grating. The grating disperses the light beam to produce the spectrum, a
portion of which is focused on the exit slit of the monochromator by a
collimating mirror. From here the beam is passed to a sample compartment
through one of the filters, which helps to eliminate unwanted second order
radiation from the diffraction grating. Upon leaving the sample compartment,
the beam is passed to the silicon photodiode detector and causes the detector
to produce an electrical signal that is displayed on the digital display.
Unpacking Instructions:
Carefully unpack the contents and check the materials against the following
packing list to ensure that you have received everything in good condition.
Packing List
Description Quantity
 Spectrophotometer .................................................. 1
 Mains Lead .............................................................. 1
 Cuvettes .................................................................. Set of 4, glass
............................................................ Set of 2, quartz
 Dust Cover............................................................... 1
 Manual..................................................................... 1
3

UV-3 SERIES SPECTROPHOTOMETER
Specifications:
Model UV-3000/
UV-3000PC
UV-3100/
UV-3100PC
UV-3200/
UV-3200PC
UV-3200S/
UV-3200PCS
UV-3300/
UV-3300PC
Wavelength Range 190-1100nm
Spectral Bandwidth 4.0nm 2.0nm 1.8nm
0.5/1.0/2.0/4.0nm 1.0nm
Optical System Single Beam, Grating 1200lines/mm
Wavelength Accuracy ±0.5nm ±0.3nm
Wavelength
Repeatability 0.3nm 0.2nm
Scanning Speed Hi, Med.,Low.Max.3000nm/min
Photometric Accuracy ±0.3%T ±0.2%T
Photometric
Repeatability ±0.2%T ±0.15%T
Photometric Range -0.3-3A, 0-200%T.0-9999Conc.
Stray Light 0.05%T
Stability ±0.002A/h 0.001A/h
Display LCD(320x240) or PC Model
Baseline Flatness ±0.002A(200-1000nm) ±0.001A(200-1000nm)
Sample Compartment Accommodates 100mm path-length cuvette with optional holder
Light Source Halogen & Deuterium lamp (pre-aligned)
Output USB Port & Parallel Port (Printer)
Power Requirement AC 220V/50Hz or AC 110V/60Hz
Dimensions (W x D x H) 480x 360 x 160mm 600x 450 x 200mm
Weight 14kg 20kg
Installation:
1. After carefully unpacking the contents, check the materials with the
packing list (page 2) to ensure that you have received everything in
good condition.
2. Place the instrument (Fig.1-3) in a suitable location away from direct
sunlight. In order to have the best performance from your instrument,
keep it as far as possible from any strong magnetic or electrical fields
or any electrical device that may generate high-frequency fields. Set
the unit up in an area that is free of dust, corrosive gases and strong
vibrations.
LCD
Control Panel
Compartmen
Holder Changer
4
Fig.1-3

UV-3 SERIES SPECTROPHOTOMETER
3. Remove any obstructions or materials that could hinder the flow of air
under and around the instrument.
4. Use the appropriate power cord and plug into a grounded outlet.
Power Switch
Fuse
Power Cord
USB Port
Fan
Parallel Port
Fig.1-4
5. Turn on your spectrophotometer. Allow it to warm up for 15 minutes
before taking any readings. We suggest you then do the Calibrate
System with the Search 656.1nm to set the wavelength to the
deuterium lamp emission line.
NOTE:
This symbol means Caution, Risk of Danger.
Operation:
Prepare the spectrophotometer
Fig 2 is the control panel. User can perform all operations by pressing the
keys and all the results and operation information are displayed on the LCD.
5

UV-3 SERIES SPECTROPHOTOMETER
6
MNO +/-/.
9
WXYZ 0
8
TUV
7
PQRS
ESC
100%T
OABS
OPEN SAVE
GOTO
λ
PRINT ENTER
F1 F2 F3 F4
1
CLEAR
4
GHI
5
JKL
3
DEF
2
ABC
START
STOP
LCD
FUNCTI
ON
KEY
NUMBER
KEY
CONTROL
KEY
Fig 2
 Description of keys
【ESC】 Exit to previous screen or cancel the operation;
【100%T /0Abs】 Blank or scan the base line;
【OPEN】 open data or curve saved before;
【SAVE】 Save data or curve;
【START/STOP】 Start testing or scanning sample;
【GOTOλ】 Set wavelength;
【PRINT】 Print test results or screen
【ENTER】 Confirm the inputted data or selected item; Go into next
setup or screen;
【F1】- 【F4】 Function based on the information on the screen;
Note: No symbols printed on the Function Keys! For convenient description in the manual, we
call them F1, F2, F3 and F4 respectively.
【0】-【9】 Input number or letter, consecutively press a numeric key
to select a character;
【+/-/.】 Input +,- or dot;
【CLEAR】 Clear all characters when you are inputting or clear curve
displays on the screen;
6

UV-3 SERIES SPECTROPHOTOMETER
【＜】,【＞】Change “x” scale; Search point after scan; 【＜】 clear a
character;
【∧】,【∨】Change “y” scale; Search peak after scan; Scroll items for
selecting; Change capital/small letter last typed in; Browse
the items for selection;
 Turn on spectrophotometer
Turn on spectrophotometer by pressing the Power Switch (IO). The
instrument starts to initiate and the steps are as below:
1.The instrument will warm up D2 lamp first(Fig 3), then initialize
com-port and printer, then start kernel, after positioning filter, auto-cell
changer(if installed) and D2 / W lamps, the screen display as Fig 3A. 15
minutes pass or press【ESC】,the screen display as Fig 4,Select “No” to skip
to main menu( Fig 6) or select “Yes”(recommended) to calibrate system (Fig
5).The calibrating process include “get dark current”, “searching 656.1nm” and
“check energy”. After finish the calibration system, go to main menu too (Fig 6).
2 .If the data in memory has been lost, the instrument will directly
calibrate system without any choice for you.
3. If no auto-cell changer installed
， “cell #1” will disappear in Fig6
Fig 3
Shanghai Mapada Instruments Co., Ltd
D2
W
Wait until EasyRTOS booted:
WL : 656.1nm D2 Lamp Warming
D2
W
Wait until EasyRTOS booted:
Init Printer . . . . . . . . . . .
√
Init Comm Port . . . . . . . .
√
Start Kernel . . . . . . . . . .
√
Positioning . . . . . . . . . .
√
Warm up 15 minutes . . . ?
WL : 656.1nm 16:20:05
Press ESC to skip ...
Init AD Converter . . . . . .
√
Filter Positioning . . . . . .
√
Lam p Positioning . . . . . .
√
Fig 3A
Note: The instruments only display one checking step at a time, not display as Fig 3A
indicated.
7

UV-3 SERIES SPECTROPHOTOMETER
D2
W
Wait until EasyRTOS booted:
WL : 656.1nm 16:20:18
System calibrating? No
Warm up 15 minutes. . . . √
Fig 4
D2
W
Wait until EasyRTOS booted:
Search 656 .1nm . . . . . . √
Check Energy. . . . . . . . . . √
Calibrate System . . . . . . . ?
WL : 656.1nm Step to 190nm
Fig 5
Note: The instruments only display one checking step at a time, not display as Fig 5
indicated.
WL : 656.1nm 16:31: 35
D2
W
Cell #1
Cancel Move Dn Select
2. Quantitative Mode
3. Wavelength Scan
4. Kinetics Mode
5. DNA/Protein Mode
7. System Utility
6. Multi-Wavelength Mode
Main Menu For UV3100
1. Basic mode
Fig 6
 Basic operation
Blank
 Push the blank cuvette into the lightpath.
 Press the key 【0Abs/100%T】for blanking
Note:1. If the reference solution is too thick, “Energy Low…” will appear
following the “Blanking…”on the screen (Fig 7).If “Energy too Low…” appears
following the “Blanking…”, the test will be paused and “Warning…”will appear
on the screen.(Fig 8).
2. If no automatic changer installed “cell #1” and “Max E” will disappear in
Fig 7
8

UV-3 SERIES SPECTROPHOTOMETER
WL : 656.1nm Energy Low. . .
Blanking
D2
W
Cell #1
Max E
Unit Mode Factor Standard
Fig 7
NOTE: 1.Blanking is automatic after a wavelength change
DO NOT OPEN SAMPLE COMPARTMENT LID DURING
BLANKING.
2. The dark current doesn’t be taken after power on, if you bypass the
calibrating system. It is recommended to take the dark current after
warm up. (See page 38)
Warning
……
D2
W
Cell #1
Max E
WL : 656.1nm Energy too Low. . .
Unit Mode Factor Standard
Fig 8
Set wavelength (Example: set wavelength in “Basic mode”)
 Press【GOTOλ】(Fig 9).
WL : 656.1nm 12: 35: 27
0.001 Abs
D2
W
Cell #1
Max E
Unit Mode Factor Standard
Fig 9
 Use numeric keypad to input wavelength (Fig 10).
9

UV-3 SERIES SPECTROPHOTOMETER
WL : 656.1nm 12: 35: 27
0.001 Abs
D2
W
Cell #1
Max E
Please input wl: 656.1
Fig 10
 Press 【ENTER】 to change the wavelength from 656.1nm to
450.0nm,and then blank; After blanking, the screen displays as Fig
11.
WL : 450.0nm 12: 35: 27
0.000 Abs
D2
W
Cell #1
Max E
Unit Mode Factor Standard
Fig 11
open or delete data or curve (Take the “WL scan” test for example)
Press 【3】in Fig.6 go into “WL scan”. After【OPEN】being pressed,
the first file (ABC.wav) in memory will appear on the bottom lines of
screen .Showed as Fig 12. Press 【∧】or【∨】 to browse the files stored
in memory. Then if
1. The key.【ENTER】be pressed, the file selected will be opened and
displays on the screen. Fig 13.
Note (1).The file selected must match “WL scan” test’s type. if not ，
the “file type error…” will appear on the right of top line.
(2).Different test has different file type. Refer to table 1 on page9.
2. The key 【CLEAR】be pressed the file selected will be deleted by
selecting ”Yes”.
10

UV-3 SERIES SPECTROPHOTOMETER
D2
W
Cell #1
WL : 680.0nm %T: 12: 35: 27
200.0 Wavelength (nm) 680.0
From:
200 .0
To:
680 .0
Step:
1 .0nm
XScale
YScale
0 %T 120.0
Setup Mode Search Smooth
Fig 12
D2
W
Cell #1
WL : 680.0nm %T: 12: 35: 27
200.0 Wavelength (nm) 680.0
From:
200 .0
To:
680 .0
Step:
1 .0nm
XScale
YScale
0 %T 120.0
Setup Mode Search Smooth
Fig 13
Table 1
Test File Type
Quantitative Curve ***.fit
Quantitative Test Result ***.qua
WL Scan ***.wav
Kinetics ***.kin
DNA/Protein ***.dna
Multi WL ***.mul
WL Validity ***. wlv
Accu. Validity ***.phv
Save data or curve (Example: Save curve in “WL scan”)
 Press the key 【SAVE】 in Fig13 to save curve.
 Name the curve by pressing the numeric keypad (Fig 14), press
the key【ENTER】 to confirm.
. Note(1). Pressing numeric key continually to scroll characters and
pressing 【∧】,【∨】to alter capital letter to miniscule.
Table 2 shows all characters built in.
(2) If the name already exists in memory, the warning
“duplicated name, are you sure ？” will appear .
“Yes”for overwrite and“No”for Exit.
11

UV-3 SERIES SPECTROPHOTOMETER
(3) The length of filename is less than 4.
D2
W
Cell #1
WL : 680.0nm %T:
12: 35: 27
200.0 Wavelength (nm) 680.0
From:
200.0
To:
680.0
Step:
1.0nm
XScale
YScale
Please input File Name:2
0 %T 120.0
Fig 14
Table 2
key representing key representing key representing
0 0,+,-,* ,/ 1 1,#,?,:,I 2 2,A,B,C,=
3 3,D,E,F,% 4 4,G,H,I,{5 5,J,K,L,}
6 6,M,N,O,~7 7,P,Q,R,S, 8 8,T,U,V,“
9 9,W,X,Y,Z +/-/. -,.,
Print test report (For example: Print the report in “Basic mode”, Fig 16)
Press the key【PRINT】to print the report (curve or data you have opened or
tested, Fig 16).
WL : 546.0nm
12: 35: 27
0.221 Abs
D2
W
Cell #1
Max E
Unit Mode Factor Standard
Fig 15
Fig 16
Before measurement
12
 Make a blank reference solution by filling a clean cuvette (or test tube) half
full with distilled or de-ionized water or other specified solvent. Wipe the

UV-3 SERIES SPECTROPHOTOMETER
cuvette with tissue to remove the fingerprints and droplets of liquid.
 Fit the blank cuvette into the 4-cell linear changer and place the cuvette in
the slot nearest you. For the ChromTech UV-3 SERIES, push the
changer so that the cuvette is in the light path (Push the rod in). Close the
lid.
Analyze Sample
For different user requirements, we have provided different test methods.
Basic Mode
Push the blank cuvette into the light path. In main menu (Fig6),press【1】to
enter “Basic mode” test . After automatically blanking, it will display as Fig 17
(automatic changer installed) or Fig 18 (automatic changer uninstalled) and
wait for the operator. 【ESC】to exit.
Note: .If no automatic changer installed “cell #1” and “Max E” will disappear
in Fig17
WL : 656.1nm 12: 35: 27
0.000 Abs
D2
W
Cell #1
Max E
Unit Mode Factor Standard
Fig17
WL : 656.1nm 12: 35: 27
0.000 Abs
D2
W
Max E
Unit Mode Factor Standard
Fig18
☺ Test
There are three modes (T%, Abs, Conc / factor) for you to select by
pressing 【F2】to make choice.
13

UV-3 SERIES SPECTROPHOTOMETER
WL : 656.1nm 12: 35: 27
0.104 Abs
D2
W
Cell #1
Max E
Unit Mode Factor Standard
Fig 19
1. Abs mode
Push the blank cuvette into the light path. Press 【F2】 to select Abs
mode ,Press 【0Abs/100%T】for Blanking , and then Push the sample into
light path to take reading(Fig 19 )
2. T% mode
The operation is the same as Abs test mode but pressing【F2】 to select
T% mode .
3. Conc/Factor mode
Press 【F1】 to select a concentration unit (Fig 20). If no unit is
suitable for your test, please select the item “Other”, press【Enter】and
input a new unit by pressing the numeric keypad (Fig 21).
WL : 656.1nm 12: 35: 27
D2
W
Cell #1
Max E
Please select unit: %
0.000 mg/ml
F factor 2.000
Fig 20
WL : 656.1nm
12: 35: 27
D2
W
Cell #1
Max E
Please input self defined unit: c
0.000 mg/ml
F factor 2.000
Fig 21
4. Push the blank cuvette into the light path and press 【0Abs/100%T】for
Blanking. There are now two choices for you to take:
4.1 Press【F3】to input known F value, Fig 22. Then push the sample into
14

UV-3 SERIES SPECTROPHOTOMETER
light path to take reading of concentration
4.2 Push sample of known concentration into the light path
Press【F4】to input known Conc value, Fig 23. Then push the sample
into light path to take reading of concentration.
Note:1.You can select wavelength at any time by pressing【GOTOλ】.
After your selection, instrument always blanks automatically.
2. If F value is more than 9999, the “out of range” will display on
screen.
WL : 656.1nm
12: 35: 27
D2
W
Cell #1
Max E
Please input F factor: 4
0.000 mg/ml
Fig 22
WL : 656.1nm
12: 35: 27
0.000 mg/ml
D2
W
Cell #1
Max E
F=4.000
Unit Mode Factor Standard
Fig 23
☺ Print Test Report
Press 【PRINT】to print test results (Fig 24).
Fig 24
Quantitative
Press【2】in Main Menu for “Quantitative” Test (Fig 25). Press【ESC】to exit.
Note: .If no automatic changer installed “cell #1” will disappear in Fig25.
15

UV-3 SERIES SPECTROPHOTOMETER
WL : 700.0nm Abs : 12: 35: 27
D2
W
Cell #1
Search
Quantitative Test
ID Abs Conc . (mg/L
)
WL
(
nm
)
700 .0
C=1.000*A^1 r=1.000
Scroll
Unit Fit Curve
Fig 25
☺ How to operation
1. Press 【F1】to select unit of concentration (Fig 26).
WL : 700.0nm Abs: 12: 35: 27
D2
W
Cell #1
Please select unit: g/L
Quantitative Test
ID Abs Conc . (mg/L
)
WL
(
nm
)
700 .0
C=1.000*A^1 r=1.000
Search
Scroll
Fig 26
2 Press 【GOTOλ】 to select correction methods and enter the
wavelength. There are three correction methods (single WL,
Iso-absorbance and 3 point, Fig 27) .
Note: Please refer to the Appendix B for the correction method.
WL : 700.0nm Abs : 12: 35: 27
D2
W
Cell #1
Quantitative Test
ID Abs Conc . (mg/L
)
WL
(
nm
)
700 .0
C=1.000*A^1 r=1.000
Correction method: Single WL
Search
Scroll
Fig 27
3.Press 【F2】in Fig 25 for more items to select .See Fig 28.
16

UV-3 SERIES SPECTROPHOTOMETER
WL : 700.0nm Abs : 12: 35: 27
D2
W
Cell #1
Calibration table
No Conc . (mg/L) Abs
WL
(
nm
)
700 .0
C=1.000*A^1 r=1.000
Method Params Standard Show Curve
Fig28
3.1Press 【F1】in Fig 28 to select fitting method. There are 4 methods for
you to choose: Linear fit, linear fit through zero, square fit and cubic fit.
3.2Press 【F2】in Fig 28 to enter directly a known standard curve.Fig29.
WL : 700.0nm Abs : 12: 35: 27
D2
W
Cell #1
Calibration table
No Conc . (mg/L) Abs
WL(nm)
700 .0
C=1.000*A^1 r=1.000
Input K1=1.033
Fig29
The constants to be entered are depending on which fitting method selected. The
table below lists their relation:
Fitting Method Fitting Equation constants
linear fit through zero C=K1×A K1, r*
Linear fit C=K0+K1×A K0,K1,r*
square fit C=K0+K1×A+K2×A2K0,K1,K2
cubic fit C=K0+K1×A+K2×A2+K3×A3K0,K1,K2,K3
* r : regression coefficients, default=1
3.3 Press 【F3】in Fig 28 to establish a standard curve by measuring a
group of standard samples. See Fig 30.
3.3.1 Enter standard concentrations of samples by pressing the
Numeric keypad followed by【ENTER】.Press 【∧】or【∨】 to
modify the inputted data (Fig31).
Press【ESC】to finish inputting and to exit (Fig 32).
17

UV-3 SERIES SPECTROPHOTOMETER
WL : 700.0nm Abs : 12: 35: 27
D2
W
Cell #1
Setup Standard Conc.
C=1.000*A^1 r=1.000
Edit the number . . .
WL
(
nm
)
700 .0
ID Abs Conc. (mg/L)
1 0.062 0.062
2 0.061 0.061
3 0.062 0.061
4 0.061 0.061
Fig 30
WL : 700.0nm Abs : 12 : 35: 27
D2
W
Cell #1
Setup Standard Conc.
C=1.000*A^1 r=1.000
Input Standard Conc: 3
WL
(
nm
)
700 .0
ID Abs Conc. (mg/L)
1 0.062 0.062
2 0.061 0.061
3 0.062 0.061
4 0.061 0.061
Fig 31
3.3.2 Push the blank cuvette into the light path, press
【0Abs/%100T】,the instrument will step to the wavelength and
blank. See Fig 32.
D2
W
Cell #1
Calibration table
C=1.000*A^1 r=1.000
WL : 700.0nm Abs : Blanking …..
WL
(
nm
)
700 .0
Method Params Standard Show Curve
ID Abs Conc. (mg/L)
1 0.062 0.062
2 0.061 0.061
3 0.062 0.061
4 0.061 0.061
Fig 32
3.3.3 Pull the first sample cuvette of known concentration into the
light path, Press the key【START/STOP】to get values of
standard curve one by one (Fig 33).
3.3.4 Press【F4】to draw the curve. You can get a different curve by
pressing【F1】 to select a different fitting method.
See Fig 34 - Fig37.
For linear fits, “ r ” represent fitting coefficient of linear
18

UV-3 SERIES SPECTROPHOTOMETER
regression .r=1 is best fitting. Usually “ r ” is very close to 1.
Note: If there are few standard samples, it is not suitable for
selecting square fitting, especially cubic fitting, otherwise invalid
fitting result will be obtained.
D2
W
Cell #1
Calibration table
C=1.000*A^1 r=1.000
No Conc . (mg/L) Abs
1 2.000 0 .247
2 3 .000 0 .375
3 4 .000 0 .532
4 5 .000 0 .603
5 6 .000 0 .764
WL : 656.1nm Abs : 12: 35: 27
WL
(
nm
)
700 .0
Method Params Standard Show Curve
Fig 33
D2
W
Cell #1
Press (ESC) to return . . .
WL : 656.1nm Abs:
12: 35: 27
-1.0 Abs 4.0
0 Conc. 76
Fig 34 linear through zero fit
D2
W
Cell #1
Press (ESC) to return . . .
WL : 656.1nm Abs:
12: 35: 27
-1.0 Abs 4.0
0 Conc. 76
Fig 35 square fit
D2
W
Cell #1
Press (ESC) to return . . .
WL : 656.1nm Abs:
12: 35: 27
-1.0 Abs 4.0
0 Conc. 91438
Fig 36 cubic fit
19

UV-3 SERIES SPECTROPHOTOMETER
D2
W
Cell #1
Press (ESC) to return . . .
WL : 656.1nm Abs:
12: 35: 27
-1.0 Abs 4.0
0 Conc. 76
Fig 37 linear fit
3.3.5 Press 【SAVE】to save calibration if required
3.3.6 Press【ESC】to exit
4. Quantitative Test
Before test, the standard curve must be obtained. There are three
ways for you to obtained it (a, b or c).
a) Standard curve built up and saved in the instrument.
In Fig 33 press【OPEN 】and then press【∧】or【∨】 to select the
file with type ***.fit. At last press【ENTER】to confirm.
b) Known standard curve, which is not saved in the instrument.
See 3.2. For Fig 28 enter a known standard curve directly.
c) Use the standard samples for the test. First the standard curve must
be established using the method shown in 3.3.
Note: All sample results must be taken in screen (Fig25).
4.1 Push the blank cuvette into the light path and press 【0Abs/100%T】for
blanking.
4.2 Pull the sample cuvette into the light path, press the key
【START/STOP】, the results will be displayed on the screen (Fig 38).
WL : 700.0nm Abs : 12: 35: 27
D2
W
Cell #1
Quantitative Test
WL
(
nm
)
700 .0
C=1.000*A^1 r=1.000
Search
Scroll
ID Abs Conc . (mg/L)
1 0.062 0.062
2 0.061 0.061
3 0.062 0.061
4 0.061 0.061
Unit Fit Curve
Fig 38
4.3 If there is more than one sample, repeat step 4.2 for the next sample
4.4 Press 【SAVE 】 to save the results and fitting parameters
20