Cole Parmer MSU-600 Series User manual

Installation and Operating Instructions
MSU-600 Series Compound Microscopes
Models 78904-16, -18, -21, -24, -27, -30, -33

Introduction ____________________________________________________________________ 2
1.0 General safety instructions ____________________________________________________ 3
1.1 Dangers associated with the operation _______________________________________________________ 3
1.2 Photobiological safety LED, important safety instructions _______________________________________ 3
1.3 Prevention of biological and infectious hazards ________________________________________________ 3
1.4 Disinfection and decontamination ___________________________________________________________ 4
2.0 Models _____________________________________________________________________ 6
3.0 Components of the microscope ________________________________________________ 7
4.0 Preparing the microscope for use_______________________________________________ 8
4.1 Assembling steps __________________________________________________________________________ 8
5.0 Operation __________________________________________________________________ 10
5.1 Setting up the illumination ________________________________________________________________ 10
5.2 Placing the specimen slide _________________________________________________________________ 10
5.3 Focusing and slide protection ______________________________________________________________ 10
5.4 Adjusting the focusing tension _____________________________________________________________ 10
5.5 Eyepieces _______________________________________________________________________________ 11
5.6 Abbe condenser __________________________________________________________________________ 12
5.7 The eld (Köhler) diaphragm (A) ____________________________________________________________ 12
5.8 Adjusting the Aperture Diaphragm (B) _______________________________________________________ 12
5.9 Use of the S100x oil-immersion objective_____________________________________________________ 12
5.10 Safety device____________________________________________________________________________ 12
5.11 Illumination _____________________________________________________________________________ 13
5.12 Auto-O Function _____________________________________________________________________ 13
6.0 Phase contrast (model 78904-24) ______________________________________________ 13
6.1 Use of phase contrast______________________________________________________________________ 13
6.2 Using the Phase contrast slider _____________________________________________________________ 13
6.3 Using the Zernike phase contrast set ________________________________________________________ 13
6.4 Centering the phase rings__________________________________________________________________ 14
7.0 Maintenance and cleaning ____________________________________________________ 15
7.1 Cleaning the optics _______________________________________________________________________ 15
7.2 Maintenance of the stand __________________________________________________________________ 15
7.3 Replacing the fuse ________________________________________________________________________ 15
8.0 Digital cameras _____________________________________________________________ 16
9.0 Accessories and spare parts___________________________________________________ 16
10.0 Warranty __________________________________________________________________ 16
11.0 Supplementary Manuals _________________________________________________ 18-27
11.1 Darkeld (Model 78904-27)______________________________________________________________18-20
11.2 Metallurgical (Model 78904-30) __________________________________________________________21-23
11.3 Polarization (Model 78904-33) ___________________________________________________________24-27
Contents
Introduction
Thank you for purchasing the Cole-Parmer MSU-600 Series compound microscope. These powerful and ergonomic
MSU-600 microscopes are ideal for life sciences, biomedical research, materials science, and universities.
Please read this manual carefully before using this product to ensure correct and safe usage
• The content of this manual is subject to change without notice
• The appearance of the actual product can dier from the models described in this manual
• Not all equipment mentioned in this manual has to be part of the set you have purchased
2

1.0 General safety instructions
Intended use: As a non-medical device
This microscope is intended for general observation of cells and tissues. The microscope is intended to be used with
transmitted/reected illumination and with the specimen xed on a slide.
1.1 Dangers associated with the operation
• Improper use could result in injury, malfunction or damage to property. It must be ensured that the operator
informs every user of existing hazards
• Danger of electrocution. Disconnect the power to the entire lighting system before installing, adding or
changing any component
• Not to be used in corrosive or explosive environments
• Avoid direct exposure of eyes to the collimated light beam or direct light from the light guides or bers
• To avoid a hazard to children, account for all parts and keep all packing materials in a safe place
1.2 Photobiological safety LED, important safety instructions
• Avoid direct eye exposure to any LED light source while it is on
• Before looking into the eyepieces of the microscope, lower the intensity of the LED illumination to a low level
• Avoid high-intensity exposure and long exposure to LED light because this can cause acute damage to the
retina of the eye
1.3 Prevention of biological and infectious hazards
Infectious or bacterial or viral biohazard substances under observation may be a risk to the health of humans and
other living organisms. Special precautions should be taken:
• Biological hazards: keep a logbook of all the biological substances or pathogenic microorganisms that were
under observation with the microscope and show it to everybody before they use the microscope or before
they do some maintenance work on the microscope! Agents can be bacterial, spores, enveloped or non-
enveloped virus particles, fungi or protozoa
• Contamination hazard:
• A sample that is properly enclosed with a cover glass, never comes in direct contact with the microscope
parts. In that case prevention of contamination lies in the handling of the slides, as long as the slides are
decontaminated before use and are treated normally and are not damaged, there is virtually zero risk of
contamination
• A sample that is mounted on a slide without cover glass, can come in contact with components of the
microscope and be a hazard to humans and/or the environment. Therefore, check the microscope and
accessories on possible contaminations. Clean the microscope surfaces and its components as thoroughly
as possible and should you identify a possible contamination, inform the local responsible person in your
organization
• Microscope operators could be contaminated from other activities and cross-contaminate components
of the microscope. Therefore, check the microscope and accessories on possible contaminations. Clean
the microscope surfaces and its components as thoroughly as possible and should you identify a possible
contamination, inform the local responsible person in your organization. It’s recommended to wear sterile
gloves when preparing the slides and manipulating the microscope in order to reduce contamination by the
operator
• Infection hazard: direct contact with the focusing knobs, stage adjustments, stage and eyepieces/tubes of
the microscope can be a potential source of bacterial and/or viral infections. The risk can be limited by using
personal eyeshades or eyepieces. You can also use personal protections such as operation gloves and/or safety
goggles which can be frequently changed to minimize the risk
• Disinfectant hazards: before cleaning or disinfection check if the room is adequately ventilated. If not,
wear respiratory protective equipment. Exposure to chemicals and aerosols can harm human eyes, skin and
respiratory system. Do not inhale vapours. During disinfection, do not eat, drink or smoke. Used disinfectants
must be disposed according to local or national regulations for health and safety
3

1.4 Disinfection and decontamination
• Exterior casing and mechanical surfaces must be wiped with a clean cloth dampened with a disinfectant
• Soft plastic parts and rubber surfaces can be cleaned by gently wiping a clean cloth dampened with a
disinfectant. Discoloration can occur if alcohol is used
• The front lens of eyepieces and objectives are sensitive to chemicals. We recommend not to use aggressive
disinfectants but to use lens paper or a soft ber-free tissue damped in cleaning solution. Cotton swabs can also
be used. We recommend you use personal eyepieces without eyeshades in order to minimize risk
• Never immerse or dip the eyepiece or objective into a disinfectant liquid! This will damage the component
• Never use abrasive compounds or cleaners that can damage and scratch coating surfaces of optics
• Clean and disinfect all possible contaminated surfaces of the microscope or contaminated accessories properly
before storing for future use. Disinfection procedures must be eective and appropriate
• Leave the disinfectant on the surface for the required exposure time, as specied by the manufacturer. If the
disinfectant evaporates before the full exposure time, reapply disinfectant on the surface
• Against bacteria, use a 70% aqueous solution of isopropanol (isopropyl alcohol) and apply for at least 30
seconds. Against viruses, we recommend to refer to specic alcohol or non-alcohol based disinfection products
for laboratories
Before returning a microscope for repair or maintenance through a Cole-Parmer dealer, a RMA (return authorization
form) and a decontamination statement must be lled in! This document must be shipped together at all times with
the microscope.
Handle with care
• This product is a high quality optical instrument. Delicate handling is required
• Avoid subjecting it to sudden shocks and impacts
• Impacts, even small ones, can aect the precision of the objective
Handling the LED
Note: Always disconnect the power cord from your microscope before handling the LED bulb and power unit and
allow the system approximately 35 minutes to cool down to avoid burns
• Never touch the LED with your bare hands
• Dirt or ngerprints will reduce the life span and can result in uneven illumination lowering the optical
performance
• Use only MSU-600 Series original replacement LEDs
• Use of other products will cause malfunctions and void warranty
• During use of the microscope the power unit will get hot; never touch it while in operation and allow the system
approximately 35 minutes to cool down to avoid burns
Dirt on the lenses
• Dirt on or inside the optical components, such as eyepieces, lenses, etc., aect the image quality of your system
negatively
• Always try to prevent your microscope from getting dirty by using the dust cover, prevent leaving ngerprints
on the lenses and clean the outer surface of the lens regularly
• Cleaning optical components is a delicate matter. Please read the cleaning instructions further on in this manual
4

Environment, storage and use
• Maximum altitude: 2000 m
• Pollution degree: 2
• This product is a precision instrument and it should be used in a proper environment for optimal use
• Install your product indoors on a stable, vibration free and level surface in order to prevent this instrument to
fall thereby harming the operator
• Do not place the product in direct sunlight
• The ambient temperature should be between 5 to +40°C and humidity should be between 20 to 80% RH.
Although the system is anti-mold treated, installing this product in a hot, humid location may still result in the
formation of mold or condensation on lenses, impairing performance or causing malfunctions
• Never turn the right and left focus knobs in opposite directions at the same time or turn the coarse focus knob
past its farthest point as this will damage this product
• Never use undue force when turning the knobs
• Make sure that the microscope system can dissipate its heat (re hazard)
• Keep the microscope approximately 15 cm free from walls and obstructions
• Never turn the microscope on when the dust cover is in place or when items are placed on the microscope
• Keep ammable uids, fabric, etc. well out of the way
Disconnect power
Always disconnect your microscope from power before doing any maintenance, cleaning, assembling or replacing
LEDs to prevent electric shocks
Prevent contact with water and other uids
Never allow water or other uids to come in contact with your microscope, this can cause short circuiting your device,
causing malfunction and damage of your system
Moving and assembling
• This microscope is a relatively heavy system, consider this when moving and installing the system
• Always lift the microscope by holding the main body and base of the microscope
• Never lift or move the microscope by its focusing knobs, stage or head
• When needed, move the microscope with two persons instead of one
5

2.0 Models
The MSU-600 Series compound microscope is available with observation methods such as brighteld, phase contrast,
and darkeld. Models with polarization and metallurgical attachments are suitable for materials science applications.
Please note: On coleparmer.com you can nd the latest updates about MSU-600 models and accessories
*The S40x, S60x and S100x objectives are equipped with a spring mount, to prevent damage to the front lens and the
slide. The Numeric Aperture - N.A. – of the objective is an indication for the resolving power of the objective
The total magnication can be calculated by multiplying the magnication of the eyepiece with the magnication of
the objective. The magnications are displayed in the table below:
Eyepiece Objective Magnication
10x 4x 40x
10x 10x 100x
10x 20x 200x
10x 40x 400x
10x 50x 500x
10x 100x 1000x
Model Type Objectives* Working
distance Stand Illumination Power Weight
78904-16 Binocular 4x/10x/S40x/S100x,
semi-plan 48 to 76 mm Rackless integrated X-Y
mechanical stage 3 W NeoLED 100–240 VAC,
50/60 Hz
24.1 lb
(10.9 kg)
78904-18 Trinocular 4x/10x/S40x/S100x,
semi-plan 48 to 76 mm Rackless integrated X-Y
mechanical stage 3 W NeoLED 100–240 VAC,
50/60 Hz
25.3 lb
(11. 5 kg)
78904-21 Trinocular 4x/10x/S40x/S100x,
plan 48 to 76 mm Rackless integrated X-Y
mechanical stage 3 W Kohler NeoLED 100–240 VAC,
50/60 Hz
25 lb
(11. 3 kg)
78904-24 Trinocular 10x/20x/S40x/S100x,
plan, phase contrast 48 to 76 mm Rackless integrated X-Y
mechanical stage 3 W Kohler NeoLED 100–240 VAC,
50/60 Hz
25 lb
(11. 3 kg)
78904-27 Trinocular 4x/10x/S40x/S100x,
plan, darkeld 48 to 76 mm Rackless integrated X-Y
mechanical stage 5 W LED 100–240 VAC,
50/60 Hz
25 lb
(11. 3 kg)
78904-30 Trinocular 5x/10x/20x/50x,
plan, metallurgical 48 to 76 mm Rackless integrated X-Y
mechanical stage
3 W Kohler NeoLED;
Epi-NeoLED illumination
100–240 VAC,
50/60 Hz
23.2 lb
(10.5 kg)
78904-33 Trinocular 5x/10x/20x/50x,
plan, polarization 48 to 76 mm 160 mm diameter
rotating stage
3 W Kohler NeoLED; 50 W
halogen Epi-illumination
100–240 VAC,
50/60 Hz
27.1 lb
(12.3 kg)
6

3.0 Components of the microscope
The names of the several parts are listed below and are indicated in the picture:
A. Camera focus adjustment part I. Dioptric adjustment
B. Trinocular tube J. Nosepiece for 5 objectives
C. Microscope head K. Objectives
D. Height adjustment condenser L. Stage with X-Y mechanical stage
E. Slide protection handle M. Condenser with iris diaphragm
F. Coaxial coarse-and ne adjustment N. X-Y stage controls
G. Light intensity adjustment knob O. Kohler iris diaphragm
H. Eyepieces P. Collector lens
Q. Auto-o sensor
A
B
C
D
E
F
G
N
M
P
O
Q
H
I
J
K
L
7

4.0 Preparing the microscope for use
Carefully remove the items from their packing and place them
on a at, rm surface. Please do not expose the microscope to
direct sun light, high temperatures, damp, dust or acute shake.
Please make sure the worktable is at and horizontal.
When moving the microscope, use the left hand to hold the
transport handle (A) at the back of the microscope and with
the right hand the bottom of the microscope.
Caution! Holding the microscope by the stage, the stage
focusing knob will damage the microscope.
Insert the power cord into the back of the microscope and use
the cable storage system to store excessive cable while in use
or the entire cable after use (B).
Caution! If the bacterial solution or water splatters over the
stage, objective or head, pull out the power cord immediately
and dry the microscope.
For safety reasons, make sure the power switch is turned o,
the plug removed before replacing the led unit or fuse.
4.1 Assembling steps
The steps mentioned below are often not necessary but described for your convenience nonetheless:
Mounting the objectives
• Rotate the coarse focusing knob to lower the stage to the lowest position
• Install the objectives into the objective nosepiece from the lowest magnication to the highest in a clockwise
direction from the rear of the microscope. When using the microscope, start by using the low magnication
objective (4X or 10X) to search for specimen and focus, and then continue with a higher magnication objective
to observe
Assembling the microscope head
• Remove the black cover from the upper side of the stand as indicated in picture A (below), using the Allen
wrench supplied with the microscope
• Remove the transparent cover on the bottom of the head (picture B)
• Place the head of the MSU-600 on the stand and x it with the Allen wrench as indicated in picture C. The
dovetail on the bottom ts into the slot on the top of the body
8

Placing the eyepieces
• Remove the cover of eyepiece tube
• Insert the eyepiece into the eyepiece tube (picture D)
Locking the eyepieces
For models without diopter adjustment, please nd the screw for locking the eyepiece on the tube ring (picture E).
Please note that location can be slightly rotated from model to model. For models with diopter adjustment, take out
the eyepiece (picture F) and look into the tube to nd the right position of screw (picture G).
The eyeshades (optional)
Each eyepiece has a rubber eyeshade. This prevents damage to the lens, and stray light. The eyeshade can simply be
slipped over the eyepiece
Connecting the power cord
The MSU-600 series supports a wide range of operating voltages: 100 to 240 VAC. Please use a grounded power
connection
• Make sure the power switch is o before connecting
• Insert the connector of power cord into the power socket, and make sure it connects well
• Insert the other connector into the mains socket, and make sure it connects well.
Do not bend or twist the power cord, it will get damaged. Use the special cord supplied by Cole-Parmer. If it’s lost or
damaged, choose one with the same specications
9

5.0 Operation
5.1 Setting up the illumination
For optimal eect in contrast and resolution one should follow the below procedure:
• Place a specimen on the object stage and focus using the 4x objective with a fully opened iris diaphragm
• Turn light intensity to the lowest position, then look through the eyepiece(s) and turn up to comfortable
intensity level
• Turn the condenser in the highest position (for phase contrast models, please set condenser to brighteld
position)
• Close the iris diaphragm, until it is just visible on the edge of the eld of view
The microscope is properly set for use with the 4x objective. For each other magnication in brighteld use this
procedure should be repeated to ensure the best balance between contrast and resolution. Phase contrast use will be
explained further on in this manual.
Caution: The maximum light intensity when using the 4x and 10x can damage the eyes!
5.2 Placing the specimen slide
• Push the arm of the specimen holder backwards
• Release the arm slowly clamping the slide with the cover glass facing up
• Rotating the X and Y-axis knob will move the specimen to the center for alignment with the center of the
objective
5.3 Focusing and slide protection
• Select the objective 4x to the optical path
• Observe the right eyepiece with right eye. Rotate the coarse focusing knob until the image appears
• Rotate the ne focusing knob for detailed focusing
• When focused with S100x objective, lock the slide protection handle. The slide protection handle protects the
slide by limiting the travel of the table. This way the objective will not touch and damage your slides
5.4 Adjusting the focusing tension
The focusing knobs can be adjusted for tension. You can set it
from light to heavy according your own preference. Please note
that when the specimen leaves the focus plane after focusing
or the stage declines by itself, the tension should be set higher.
To tighten the focusing arm (more heavy), rotate the tension
adjustment ring according to the arrowhead pointed; to loosen
it, please turn it in the reverse direction.
Focus tension
Slide protection
10

5.5 Eyepieces
Using a binocular (or trinocular) tube is less tiring for the eyes than the use of a monocular tube. In order to obtain a
smooth “compound” image, one should go through the steps below.
5.5.1. The interpupillary distance
The correct interpupillary distance is reached when one round image is seen in the eld of view (see image below).
This distance can be set by either pulling the tubes towards each other or pulling them from each other. This distance
is dierent for each observer and thus should be set individually. When more users are working with the microscope
it is recommended to remember your interpupillary distance for a quick set-up during new microscopy sessions. The
MSU-600’s swiveling eyepiece tube can be rotated 360º. You can select corresponding eye point height according to
your own preference.
5.5.2. The correct eye point
The eye point is the distance from the eyepiece to the user’s pupil. To obtain the correct eye point, move the eyes
towards the eyepieces until a sharp image is reached at a full eld of view.
5.5.3. Adjusting the diopter(s)
In order to compensate for human eye dierences, distortion, thickness dierences in cover glasses and tune for the
best parfocality between objectives, one can use the diopter to do so. Take a good prepared slide for your reference:
5.5.3.1 Microscope models equipped with one diopter adjustment
• Position the diopter marking on the zero point
• Look into both eyepieces and focus on the specimen
• Close the right eye and look into the eyepiece with diopter adjustment, rotate the diopter adjustment from “+”
to “–“ until the selected area get as sharp as possible
5.5. 3.2 Microscope models equipped with two diopter adjustments
• Set (both) the diopter adjustments of the eyepieces to “0”
• Select the 10x objective, look for a interesting area on the specimen and focus on this area
• Select the 40x objective and focus on the specimen
Warning: don’t change the coarse and ne adjustment any more.
• With your dominant eye open (close your other eye), rotate the diopter adjustment from “+” to “–“ until the
selected area get as sharp as possible as with the 40x objective
• If during this operation the image becomes unsharp, take your eyes from the eyepieces and turn the diopter
adjustment, without looking into the eyepieces, a few divisions back from “-” to “+“ .
• Look into the eyepieces again and turn the diopter adjustment from “+“ to “-” until the selected area on your
specimen gets the optimal sharpness
• Repeat for your non-dominant eye, and with the second diopter
Verication:
• Take your eyes from the eyepieces and look for 2 seconds to a far point in the room in order to “reset” your eyes
• Look again into the eyepieces. If the adjustment is not good, repeat the operation until you reach the same
sharpness for the 10x and 40x objective without touching the coarse and micrometric adjustments
Field of view
before adjustment
Field of view
after adjustment
11

5.6 Abbe condenser
Beneath the object stage an Abbe condenser N.A. 12.5 is mounted. The condenser is factory pre-centered but can be
adjusted in height by means of a rack and pinion movement and knob. With this, one can focus the light on the specimen
by which the contrast can be optimized. If needed the following procedure can be used to center the condenser:
1. Move the condenser to the highest position
2. Select the 10x objective into the light path and focus the specimen
3. Rotate the eld diaphragm adjustment ring to put the eld diaphragm to the smallest position
4. Rotate the condenser up/down knob, and adjust the image to be clearest
5. Adjust the center adjustment screw and put the image to the center of the eld of view
6. Open the eld diaphragm gradually. If the image is in the center all the time and inscribed to the eld of view,
the condenser has been centered correctly
5.7 The eld (Köhler) diaphragm (A)
By limiting the diameter of the beam entering the condenser, the
eld diaphragm can prevent stray light and enhance the image
contrast. When the image is just on the edge of the eld of view, the
objective can show the best performance and obtain the clearest
image. The diaphragm is factory pre-centered.
5.8 Adjusting the Aperture Diaphragm (B)
• The diaphragm is used to adjust the Numerical Aperture of the
condenser. When the N.A. of the condenser matches the N.A.
of the objective, the highest possible resolution is obtained
• When contrast is low, rotate the diaphragm adjustment ring
to 70% to 80% of the N.A. of objective. This will improve the
contrast of the image. The diaphragm is factory pre-centered.
5.9 Use of the S100x oil-immersion objective
The MSU-600 Series is equipped with an S100x N.A. 1.25 oil
immersion objective. Please follow these instructions for use:
1. Remove the dust protection from the revolving nosepiece
to mount the S100x objective
2. Focus the image with the S40x objective
3. Turn the revolving nosepiece so the S100x objective almost reaches the click-stop
4. Put a small drop of immersion oil on the center of the slide
5. Now turn the S100x objective so that you feel the click stop
6. The front lens is in contact with the immersion oil
7. Look through the eyepiece and focus the image with the ne adjustment knobs
8. The distance between the lens of the objective and the slide is very small!
9. In case there are small bubbles visible turn the S100x objective a couple of times left/right so that the front of
the objective moves in the oil and the bubbles will disappear
10. After using the S100x objective turn the table with the ne adjustment knobs downwards until the front lens
doesn’t touch the oil any longer
11. Always clean the front lens of the S100x objective with a lens paper moistened with a drop of isopropanol
12. Clean the slide after use as well
5.10 Safety device
To prevent damage to the objective lens or the slide, all types are equipped with a pre-xed safety device. It is
recommended to use slides of 1.0 – 1.2 mm thickness in combination with cover glasses of 0.13 mm or 0.17 mm thickness.
12

5.11 Illumination
The illumination has the following specications:
• LED : 3W NeoLED
• Power supply : Primary 100–240 VAC, 50/60 Hz
5.12 Auto-O Function
After 20 to 30 minutes of non-use, the light source will turn o automatically.
The indicator LED (1) will ash once every 3 seconds. To turn the light back on,
press function button (2). To turn o the auto-o function, press the button
(2) for 3 seconds. This will cause the red indicator LED (1) to turn o and the
microscope light to stay on. Press the button for another 3 seconds, it will
make indicator LED (1) ash and the auto-o function is back on.
6.0 Phase contrast (model 78904-24)
6.1 Use of phase contrast
The phase contrast method was
designed in 1934 by the Dutchman
Frits Zernike to observe very thin or
transparent objects. This technique
uses the fact that light travelling
through tissue undergoes a phase
shift due to diraction.
By recombining the phase shifted
light with the background light, a
contrasted image appears in the
eyepiece.
6.2 Using the Phase contrast slider
• Keep the phase contrast slider face up (text up); insert it from left to right into the condenser slider socket as the
direction of the arrow pointed
• Each slider has 3 positions, 2 phase contrast positions and in the center of the slide the brighteld position
for normal use without phase contrast. Each phase contrast objective used has to be matched with the phase
contrast ring on the slider. For example: when the 10x phase contrast objective is used the slider should be
positioned to match the 10 phase diaphragm.
Note: The phase diaphragms in the sliders are pre-centered and do not need to be adjusted in operation
6.3 Using the Zernike phase contrast set
Any MSU-600 model with a Zernike phase contrast set comes with the condenser and objectives already mounted and
centered on your microscope. If you suspect misalignment or want to check the alignment please see the next section
for ”6.4 Centering the phase rings”. The height of condenser can be adjusted in height by means of a rack and pinion
movement. In this way the light beam is concentrated in the specimen for an optimum resolution.
13

When the condenser is in the DF or BF position the objectives can
be used for either darkeld or bright eld. For phase contrast, the
condenser position should match the objective used. Meaning
that when the condenser is in position “40” the objective used
should also be 40x
• Rotate 10× innity plan phase contrast objective into the
eld of view, then set the condenser to match the objective
(marker “10/20”)
• Take the eyepiece out of the tube and insert the centering telescope in its place. Observed from centering
telescope, the dark and bright ring images should coincide with each other as shown in the gures below. If the
ring images can’t be observed clearly, rst try and focus the centering telescope. If this does not solve the issue
raise or decline the condenser
• If the bright ring and dark ring images are not coincided as shown below, adjust the position of the ring with the
two screw keys on the side of the condenser to move the ring until bright and dark ring images superimpose.
Repeat for all objectives/Zernike disc positions
Not centered Centered properly
6.4 Centering the phase rings
The Zernike phase disc has ve positions:
• “DF” for darkeld observation (up to 400x),
• “BF” for brighteld observation, this position also has an iris diaphragm
• And “10/20”, “40” “100” which are corresponding to phase contrast observation using 10×, 20×, 40×, 100×
objectives respectively
Centration screws
Zernike condenser position indication
14

All moving parts - like the height adjustment or the coaxial course and ne adjustment - contain ball bearings that are
not dust sensitive. With a drop of sewing-machine oil the bearing can be lubricated
7.3 Replacing the fuse
To change the fuse, following the procedure below:
• Unplug the system from power and place the microscope with back toward you
• Find the fuse cover that will appear as a round protrusion with a slot
• Use a small athead screwdriver or other at object (coin, etc) to gently push the fuse cover in and turn the
cover counter clockwise. You need to turn the cover about 3/4 of a turn
• The fuse cover will pop out with the fuse attached
• Remove the fuse from the cover and examine the fuse. If the thin piece of metal going from one end of the fuse
to the
other has a gap, then the fuse is bad
• If the fuse is bad, install a replacement fuse in the cover
• Gently push the fuse cover with the new fuse back into the
body until it is ush with the unit. Turn the cover clockwise
about 3/4 to secure the cover back into the unit
• Fuse specication: 250v 500mA
Note: Fuse may blow in order to prevent internal damage to the
microscope. And in most cases, replacing with a correctly rated fuse
will resolve the issue. However, should you encounter a blown fuse
frequently, please contact your distributor for further assistance
7.0 Maintenance and cleaning
Always place the dustcover over your MSU-600 microscope after use. Keep the eyepieces and objectives always
mounted on the microscope to avoid dust entering the instrument
7.1 Cleaning the optics
When the eyepiece lens or front lens of the 10x or S40x objective are dirty they can be cleaned by wiping a piece of
lens paper over the surface (circular movements). When this does not help put a drop of alcohol on the lens paper.
Never put isopropanol or alcohol directly on the lens!
It is not necessary – and not recommended – to clean the lens surfaces at the inner side of the objectives. Sometimes
dust can be removed with high pressured air. There will never be dust in the objectives if the objectives are not
removed from the revolving nosepiece
Caution
Cleaning cloths containing plastic bers can damage the coating of the lenses!
7.2 Maintenance of the stand
Dust can be removed with a brush. In case the stand or table is really dirty the surface can be cleaned with a non-
aggressive cleaning product
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1
2
3
g. 1
g. 2
8.0 Digital cameras
Digital cameras are designed to be used on the photo port of the
microscope head (g. 1). It is also possible to use the digital camera
in combination with a binocular head (g.2). For MSU-600 Series,
simply remove the eyepiece [1] and place the 30mm adapter ring
into the eyepiece tube [2] then place the camera with mounted
c-mount adapter in the eyepiece tube [3]. Focus the digital image
with the coarse and ne controls of the microscope.
For trinocular models, slide the camera with mounted C-mount
adapter into the 23.2 mm tube of the photo port. For focussing,
slowly unscrew the tube (A) you will be able to match parfocality
of the camera with the view through the eyepieces by moving the
camera up and down inside the 23.2 mm tube. Take an easy-to-
view specimen and focus the image through the microscope’s
eyepieces (with diopter adjustment set on “0”). Afterwards,
perform the height adjustment procedure above while watching
the image on the computer screen. In this case, once you have
obtained parfocality in the device, tighten screw (A) again. Screw
(B) is only used to x the 23.2mm tube on the photo port.
Follow the manual that comes with the camera for camera
operation
9.0 Accessories and spare parts
For current accessories and spares, please visit website coleparmer.com
10.0 Warranty
These microscopes come with a ve (5) year factory warranty against manufacturing defects, covering labor and
parts. Warranty does not apply for consumable accessories and parts such as bulbs, batteries, fuses, cords, optical
components, or any add-on accessories such as mechanical specimen holders that are not built into the microscope
stage as an integral part of the original manufacture, etc. Warranty does not cover microscopes, lenses cameras or
other accessories that have become inoperable due to dirt or damage due to misuse or lack of maintenance.
Note: Buyers are responsible for return shipping and handling cost for warranty services. Warranty covers parts and
labor only.
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Notes
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11.0 Supplementary Manuals
11.1 Supplementary Manual for Darkeld Model 78904-27
18

5. Focus the image with the least magnication
(probably with 4x lens)
11.1 Supplementary Manual for Darkeld Model 78904-27 (cont.)
Assembly and use of a darkeld condensor
Installation and setting
1. Remove the brighteld condenser by unwinding
the screw marked with the arrow. Set up the dark
eld condenser and tighten the screw
3. Lower the condenser slightly, place the slide. In
this way, the immersion oil should not yet touch
the object rinse
4. Gently turn the condenser up again. When the oil
touches the slide, it is clearly visible through the
slide
2. Turn the condenser to the highest position and
place a drop of immersion oil on the lens
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A B
6. Center the condenser with the screws of the
condenser
7. The condenser is now ready for use
8. To use the 100x lens and/or the special 100x
lens with built-in iris aperture, you must also put
immersion oil on the prepared slided
9. Focus the lens. On the S100x/1.25 oil immersion
objective (A) and S100x/1.25 IOS super contrast
objective (B) you can adjust the background by
changing the opening of the inner lens aperture.
The aperture canbe changed by turning the ring
11.1 Supplementary Manual for Darkeld Model 78904-27 (cont.)
20