Cole Parmer MSU-200 Series User manual

Installation and Operating Instructions
MSU-200 Series Compound Microscope
Model 78904-00

Introduction ____________________________________________________________________ 2
General safety instructions ________________________________________________________ 3
Dangers associated with the operation ___________________________________________________________ 3
Photobiological safety LED, important safety instructions ____________________________________________ 3
Prevention of biological and infectious hazards ____________________________________________________ 3
Disinfection and decontamination: ______________________________________________________________ 4
Model with rechargeable batteries _______________________________________________________________ 5
Environment, storage and use ___________________________________________________________________ 5
Models _________________________________________________________________________ 6
Components of the microscope ____________________________________________________ 7
Preparing the microscope for use __________________________________________________ 8
Assembling steps _____________________________________________________________________________ 8
Operation ______________________________________________________________________ 9
Setting up the illumination _____________________________________________________________________ 9
Placing the specimen slide ______________________________________________________________________10
Focusing and slide protection ___________________________________________________________________10
Adjusting the focusing tension __________________________________________________________________10
Eyepieces ____________________________________________________________________________________10
Abbe condenser ______________________________________________________________________________11
The eld (Köhler) diaphragm ____________________________________________________________________11
Adjusting the aperture diaphragm _______________________________________________________________11
Use of the S100x oil-immersion objective __________________________________________________________12
Safety device _________________________________________________________________________________12
Illumination __________________________________________________________________________________12
Maintenance and cleaning _______________________________________________________ 12
Cleaning the optics ____________________________________________________________________________12
Maintenance of the stand ______________________________________________________________________13
Changing the batteries _________________________________________________________________________13
Replacing the fuse ____________________________________________________________________________13
Accessories ____________________________________________________________________ 13
Spare Parts ____________________________________________________________________ 13
Warranty ______________________________________________________________________ 13
Contents
Introduction
Thank you for purchasing the Cole-Parmer MSU-200 Series compound binocular microscope. These entry-level upright
microscopes are developed for use at schools and laboratories. They oer corded and cordless battery operation for
added convenience.
Please read this manual carefully before using this product to ensure correct and safe usage:
• The content of this manual is subject to change without notice
• The appearance of the actual product can dier from the models described in this manual
• Not all equipment mentioned in this manual has to be part of the set you have purchased
• All optics are anti-fungus treated and anti-reection coated for maximum light throughput
2

General safety instructions
Intended use: A non-medical device
This microscope is intended for general observation of cells and tissues, with transmitted/reected illumination and
with the specimen xed on a slide.
Dangers associated with the operation
• Improper use could result in injury, malfunction or damage to property. It must be ensured that the operator
informs every user of existing hazards
• Danger of electrocution. Disconnect the power to the entire lighting system before installing, adding or
changing any component
• Not to be used in corrosive or explosive environments
• Avoid direct exposure of eyes to the collimated light beam or direct light from the light guides or bers
• To avoid a hazard to children, account for all parts and keep all packing materials in a safe place
Photobiological safety LED, important safety instructions
• Avoid direct eye exposure to any LED light source while switched on
• Before looking through the eyepieces of the microscope, lower the intensity of the LED illumination
• Avoid long and high-intensity exposure to LED light because this may cause acute damage to the retina of the eye
Prevention of biological and infectious hazards
Infectious, bacterial or viral biohazard substances under observation may be a risk to the health of humans and other
living organisms. Special precautions should be taken during in vitro medical procedures:
• Biological hazards: Keep a logbook of all the biological substances or pathogenic microorganisms that were
under observation with the microscope and show it to everybody before they use the microscope or before
they do some maintenance work on the microscope! Agents can be bacterial, spores, enveloped or non-
enveloped virus particles, fungi or protozoa.
• Contamination hazard:
• A sample that is properly enclosed with a cover glass never comes in direct contact with the microscope
parts. In that case prevention of contamination lies in the handling of the slides; as long as the slides
are decontaminated before use and are undamaged and treated normally, there is virtually zero risk of
contamination.
• A sample that is mounted on a slide without cover glass, can come in contact with components of the
microscope and may be a hazard to humans and/or the environment. Therefore, check the microscope and
accessories on possible contaminations. Clean the microscope surfaces and its components as thoroughly
as possible. Should you identify a possible contamination, inform the local responsible person in your
organization.
• Microscope operators could be contaminated from other activities and cross-contaminate components
of the microscope. Therefore, check the microscope and accessories on possible contaminations. Clean
the microscope surfaces and its components as thoroughly as possible. Should you identify a possible
contamination, inform the local responsible person in your organization. it is recommended to wear sterile
gloves when preparing the slides and handling the microscope in order to reduce contamination by the
operator.
• Infection hazard: Direct contact with the focusing knobs, stage adjustments, stage and eyepieces/tubes of
the microscope can be a potential source of bacterial and/or viral infections. The risk can be limited by using
personal eyeshades or eyepieces. You can also use personal protections such as operation gloves and/or safety
goggles, which should be changed frequently to minimize the risk.
• Disinfectant hazards: Before cleaning or disinfecting, check if the room is adequately ventilated. If not, wear
respiratory protective gear. Exposure to chemicals and aerosols can harm human eyes, skin and respiratory
system. Do not inhale vapours. During disinfection, do not eat, drink or smoke. Used disinfectants must be
disposed of according to local or national regulations for health and safety.
3

Disinfection and decontamination:
• Exterior casing and mechanical surfaces must be wiped with a clean cloth, dampened with a disinfectant
• Soft plastic parts and rubber surfaces can be cleaned by gently wiping a clean cloth, dampened with a
disinfectant. Discoloration can occur if alcohol is used
• The front lens of eyepieces and objectives are sensitive to chemicals. We recommend not to use aggressive
disinfectants but to use lens paper or a soft ber-free tissue, damped in cleaning solution. Cotton swabs may
also be used. We recommend you use personal eyepieces without eyeshades in order to minimize risk
• Never immerse or dip the eyepiece or objective into a disinfectant liquid! This will damage the component
• Never use abrasive compounds or cleaners that may damage and scratch optical coatings
• Properly clean and disinfect all possible contaminated surfaces of the microscope or contaminated accessories
before storing for future use. Disinfection procedures must be eective and appropriate
• Leave the disinfectant on the surface for the required exposure time, as specied by the manufacturer. If the
disinfectant evaporates before the full exposure time, reapply disinfectant on the surface
• Against bacteria, use a 70% aqueous solution of isopropanol (isopropyl alcohol) and apply for at least 30
seconds. Against viruses, we recommend to refer to specic alcohol or non-alcohol based disinfection products
for laboratories
Before returning a microscope for repair or maintenance through a Cole-Parmer dealer, an RMA (return authorization
form) together with a decontamination statement must be lled in! This document must be shipped together with the
microscope at all times
Handle with care
• This product is a high quality optical instrument. Delicate handling is required
• Avoid subjecting it to sudden shocks and impacts
• Impacts, even small ones, can aect the precision of the instrument
Handling the LED
Note: Always disconnect the power cord from your microscope before handling the LED bulb and power unit and
allow the system to cool down approximately 35 minutes to avoid burns
• Never touch the LED with your bare hands
• Dirt or ngerprints will reduce the life span and can result in uneven illumination, lowering the optical
performance
• Use only original MSU-200 Series replacement LEDs
• The use of other products may cause malfunctions and will void warranty
• During use of the microscope the power unit will get hot; never touch it while in operation and allow the system
to cool down approximately 35 minutes to avoid burns
Dirt on the lenses
• Dirt on or inside the optical components, such as eyepieces, lenses, etc., aects the image quality of your system
negatively
• Always try to prevent your microscope from getting dirty by using the dust cover, prevent leaving ngerprints
on the lenses and clean the outer surface of the lens regularly
• Cleaning optical components is a delicate matter. Please, read the cleaning instructions further on in this
manual
4

Model with rechargeable batteries
• Always disconnect the power cord from the microscope before you replace the rechargeable batteries
• The rechargeable batteries must not be thrown away as regular trash but should be taken to special waste
collection sites, according your local or national regulations
• Risk of explosion: when removing the rechargeable batteries, do not throw the batteries into re or any other
heat source
• Do not replace the rechargeable batteries with non-rechargeable batteries
• Avoid extreme environmental conditions and temperatures which could aect the rechargeable batteries and
lead to re, explosion or leakage of hazardous substances
• If the rechargeable batteries have leaked, avoid contact of the chemicals with skin, eyes and mucous
membranes
• When in contact with the chemicals, ush the aected areas immediately with plenty of fresh water and seek
medical attention
Environment, storage and use
• Maximum altitude: 2000 m
• Pollution degree: 2
• This product is a precision instrument and it should be used in a proper environment for optimal use
• Install your product indoors on a stable, vibration free and level surface in order to prevent this instrument to
fall thereby harming the operator
• Do not place the product in direct sunlight
• The ambient temperature should be between 5 to +40°C and humidity should be within 20 to 80% RH
• Although the system is anti-mold treated, installing this product in a hot, humid location may still result in the
formation of mold or condensation on lenses, impairing performance or causing malfunctions
• Never turn the right and left focus knobs in opposite directions at the same time or turn the coarse focus knob
past its farthest point as this will damage this product
• Never use undue force when turning the knobs
• Make sure that the microscope system can dissipate its heat (re hazard)
• Keep the microscope away from walls and obstructions for at least approximately 15 cm
• Never turn the microscope on when the dust cover is in place or when items are placed on the microscope
• Keep ammable uids, fabric, etc. well out of the way
Disconnect power
Always disconnect your microscope from power before doing any maintenance, cleaning, assembling or replacing
LEDs to prevent electric shocks
Prevent contact with water and other uids
Never allow water or other uids to come in contact with your microscope, this can cause short circuiting your device,
causing malfunction and damage to your system
Moving and assembling
• This microscope is a relatively heavy system, consider this when moving and installing the system
• Always lift the microscope by holding the main body and base of the microscope
• Never lift or move the microscope by its focusing knobs, stage or head
• When needed, move the microscope with two persons instead of one
5

Models
The binocular MSU-200 Series microscope comes equipped with 30° inclined tubes, wideeld WF 10x/18 mm
eyepiece(s) and semi-plan-objectives, and height-adjustable Abbe condenser N.A. 1.25 with iris diaphragm and lter
holder.
Please note: Visit coleparmer.com to nd the latest updates about the MSU-200 Series model and accessories
The S40x and S100x objectives are equipped with a spring mount, to prevent damage to the front lens and the slide.
The Numeric Aperture - N.A. – of the objective is an indication for the resolving power of the objective. The total
magnication can be calculated by multiplying the magnication of the eyepiece with the magnication of the
objective. The magnications are displayed in the table below:
Eyepiece Objective Magnication
10x 4x 40x
10x 10x 100x
10x 40x 400x
10x 100x 1000x
Model Type Magnication Working distance Stand Illumination Power Weight
78904-00 Binocular 4/10/S40/S100x 48 to 75 mm Mechanical X-Y Replaceable
1 W NeoLED
100–240 V, 50/60 Hz adapter
and internal rechargeable
batteries with charger
8.8 lb (4.0 kg)
6

A
B
C
D
E
N
F
O
M
L
K
J
G
HI
Components of the microscope
The names of the several parts are listed below:
AStand arm ITube (mono/bino/trino 360° rotatable)
B Safety device J Revolving nosepiece for 4 objectives
CTension adjustment KObjectives
D Coaxial coarse-and ne adjustment L Object stages (mechanical stage or clips)
E On/O switch (not visible) M Condenser with iris diaphragm + lter holder
FLight intensity control N Coaxial stage controls
GEyepiece(s) OLamp housing
HDioptric adjustment (bino/trino type)
7

Preparing the microscope for use
Carefully remove the items from its packaging and place them on a at, rm surface. Please do not expose the
microscope to direct sunlight, high temperatures, damp, dust or acute shakes. Make sure the table or surface is at and
horizontal
When moving the microscope, use the left hand to hold the transport handle and hold the base of the microscope
with the right hand
Caution! Hold the microscope at the top of the stand
arm when it is moved. Holding the microscope by its
stage or focusing knobs will damage the microscope
Caution! If the bacterial solution or water splashes over
the stage, objective or head, pull out the power cord
immediately and dry the microscope
Assembling steps
We always try to keep the number of assembly steps as low as possible but in some cases there are some steps to be
taken. The steps mentioned below are often not necessary but described for your convenience nonetheless
Mounting the objectives
• Rotate the coarse focusing knob to lower the stage to its lowest position
• Install the objectives into the objective nosepiece from the lowest magnication to the highest in a clockwise
direction from the rear side of the microscope. When using the microscope, start using the low magnication
objective (4X or 10X) to bring your specimen into focus and then continue with a high magnication objective
to observe
Placing the eyepieces
• Remove the cover of the eyepiece tube
• Insert the eyepiece into the eyepiece tube
• Lock the eyepieces with an Allen wrench
8

Locking the eyepieces
To lock the eyepieces in binocular models, please nd the screw as indicated in picture (A). Please note that the
location can be slightly rotated.
The eyeshades (optional)
The eyepieces can be equipped with optional rubber eyeshades. These prevent damage to the lens and stray light. The
eyeshade can simply be slipped over the eyepiece
Connecting the power cord
The MSU-200 Series support a wide range of operating voltages: from 100 to 240 VAC Please use a grounded power
connection
• Make sure the power switch is o before connecting
• Insert the connector of the power cord into the power socket and make sure it connects well
• Insert the other connector into the mains socket and make sure it connects well
Do not bend or twist the power cord, it may get damaged. Use the power cord that is supplied by Cole-Parmer. If it is
lost or damaged, choose one with the same specications
Replacement of the Power Cord
If the power cord requires replacement, ensure that it is suitably rated. If in doubt consult your local dealer or qualied
electrician
Operation
Setting up the illumination
For optimal contrast and resolution one should follow the below procedure:
• Place a specimen on the object stage and focus using the 4x objective, with a fully opened iris diaphragm
• Turn light intensity to lowest position, then look through the eyepiece(s) and turn up to a comfortable light
intensity level
• Turn the condenser in the highest position
• Close the iris diaphragm, until it is just visible on the edge of the eld of view
The microscope is properly set for use with the 4x objective. For each other magnication in brighteld use, this
procedure should be repeated to ensure the best balance between contrast and resolution
Caution:
The maximum light intensity when using the 4x and 10x may cause damage to the eyes!
9

Placing the specimen slide
Slides go into the clamp of the mechanical stage (N) where it can be moved with precision into X- and Y- directions
• Push the arm of the specimen holder backwards
• Release the arm slowly clamping the slide with the cover glass facing up
• Rotating the X and Y-axis knob will move the specimen to the center for alignment with the center of the
objective
Focusing and slide protection
• Select the 4x objective and make sure that it is placed correctly in the optical path
• Move the stage to the top by using the coarse adjustment knob and focus till the image appears
• Rotate the ne focusing knob to bring the image into focus
• When you focus with a S100x objective, you need to lock the slide protection handle. The slide protection
handle protects the slide by limiting the travel range of the mechanical stage. This way the objective will not
touch or break your slides
Adjusting the focusing tension
The tension of the focusing knobs can be adjusted. You can set it from light to heavy according to your own
preference. Please note that when the specimen leaves the focus plane after focusing or the stage declines out of its
own, then you need to adjust the tension. To tighten the focusing knob (more heavy), rotate the tension adjustment
ring counter-clockwise; to loosen it, turn it in the clockwise direction.
Eyepieces
Using a binocular (or trinocular) e, we recommend you to go through the steps below:
1. The interpupillary distance
The correct interpupillary distance is reached when one round image is seen in the eld of view (see image below).
This distance can be set by either pushing the tubes towards each other or pulling them away from each other. This
distance is dierent for each observer and thus should be set individually. When more users are working with the
microscope it is recommended to remember your interpupillary distance for a quick set-up during new microscopy
sessions
2. The correct eye point
The eye point is the distance from the eyepiece to the user’s pupil. To obtain the correct eye point, move the eyes
towards the eyepieces until a sharp image is reached at a full eld of view
Field of view
before adjustment
Field of view
after adjustment
10

3. Adjusting the diopter
• Set diopter adjustment ring to zero
• Close the left eye and focus the right tube by adjusting the coarse- and ne adjustment knobs
• Close the right eye and focus the left tube with the diopter adjustment ring
This procedure should be followed by each individual user. When more users are working on the same microscope it is
recommended to remember your own diopter setting for a quick set-up during new microscopy sessions
Abbe condenser
Beneath the object stage an Abbe condenser N.A. 1.25 is mounted. The condenser can be adjusted in height by
moving the lever beneath the mechanical stage. By adjusting the condenser you can focus the light on the specimen
for an optimized contrast. The condenser is factory pre-centered. If needed, the following procedure can be followed
to center the condenser
1. Move the condenser to its highest position
2. Select the 10x objective and place it into the light path and focus on the specimen
3. Rotate the eld diaphragm adjustment ring to put the eld diaphragm to the smallest position
4. Adjust the condenser to get the sharpest image
5. Adjust the center adjustment screw and center the image in the eld of view
6. Open the eld diaphragm gradually
7. The condenser is centered correctly if the image remains in the center when you open the eld diaphragm
The eld (Köhler) diaphragm
By limiting the diameter of the beam entering the condenser, the eld diaphragm can prevent stray light and increase
the image contrast. When the image is right on the edge of the eld of view, the objective shows the best performance
and obtains the clearest image. The diaphragm is factory pre-centered
Adjusting the aperture diaphragm
1. The aperture diaphragm is used to select the
numerical aperture of the illumination. When the
N.A. of the illumination matches with the N.A.
of the objective, you get the highest possible
resolution, depth of eld and contrast
2. When contrast is low, rotate the diaphragm
adjustment ring to 70%-80% of the N.A. of
objective. This will improve the contrast of the
image. The diaphragm is factory pre-centered
11

Use of the S100x oil-immersion objective
Some Cole-Parmer MSU-200 Series microscopes are equipped with an S100x N.A. 1.25 oil immersion objective. Please
follow the instructions below on how to use this objective:
1. Remove the dust protection cap from the revolving nosepiece to mount the S100x objective
2. Focus the image with the S40x objective
3. Lock the slide protection handle
4. Turn the revolving nosepiece so that the S100x objective almost reaches the click-stop
5. Put a small drop of immersion oil on the center of the slide
6. Now turn the S100x objective so that you feel the click stop
7. The front lens is in contact with the immersion oil
8. Look through the eyepiece and focus on the image with the ne adjustment knobs
9. The distance between the lens of the objective and the slide is very small!
10. In case there are small bubbles visible, turn the S100x objective a couple of times from left to right so that the front
of the objective moves in the oil and the bubbles will disappear
11. After using the S100x objective, loosen the slide protection handle and turn the table with the course
adjustment knobs downwards until the front lens no longer touches the oil. Clean the front lens of the S100x
objective
12. Always clean the front lens of the S100x objective with a piece of lens paper that is moistened with a drop of
isopropanol.
13. Clean the slide after use as well
Caution
• Never put a drop of xylol or alcohol directly on the lens of the objective. It may enter the objective and
dissolve the glue that holds the lenses!
• Avoid oil contact with any of the other objectives!
Safety device
All types are equipped with a pre-xed safety device to prevent damage to the objective lens or the slide
It is recommended to use slides with a thickness of 1.0 – 1.2 mm in combination with cover glasses with a thickness of
0.13 mm or 0.17 mm
Illumination
The LED illumination of the MSU-200 Series is equipped with rechargeable batteries. The length of use after charging
is about 60 hours. The full charging time is about 10 hours. Before rst use the batteries have to be fully charged.
Connect the cable to the mains socket. The illumination has the following specications:
• LED : 1 W, 300 mA
• Charger : Primary 100-240 V, 50/60 Hz, 5 W
• Batteries : 3 NiMh, AA type, 1.2 Volt 1600 - 2600 mA
Maintenance and cleaning
Always cover your MSU-200 Series microscope with a dust cover after use. Always keep the eyepiece(s) and objectives
mounted on the microscope to avoid dust entering the instrument
Cleaning the optics
When the eyepiece lens or front lens of the 10x or S40x objective are dirty, they can be cleaned by wiping a piece of
lens paper over the surface (circular movements). When this does not help put a drop of alcohol on the lens paper and
wipe it. Never put xylol or alcohol directly on the lens!
12

It is not necessary – and not recommended – to clean the lens surfaces at the inside of the objectives. Sometimes dust
can be removed with high pressured air. There will never be dust in the objectives if the objectives are not removed
from the revolving nosepiece
Caution
Cleaning cloths containing plastic bers may damage the coating of the lenses!
Maintenance of the stand
Dust can be removed with a brush. In case the stand or table is really dirty you can clean the surface with a non-
aggressive cleaning agent. All moving parts -like the height adjustment or the coaxial course and ne adjustment- -
contain ball bearings that are not dust sensitive. With a drop of sewing-machine oil you can lubricate the bearing.
Changing the batteries
Caution:
Always remove the power cable from the mains supply!
• Remove the small lid out of the bottom cover of the microscope
• Place the batteries and put the lid back into place
Replacing the fuse
To change the fuse, please follow these procedures:
• Remove the power cord from the back of the microscope
• Locate the fuse compartment, which has a fuse image. It is typically located beneath the
power connector
• Remove the fuse compartment. To do so, insert a at head screwdriver between the metal
power tines and gently pry the fuse compartment loose with a slight downward and
outward motion
• Insert the new fuse into the compartment, and replace the fuse compartment
• Power up the microscope and test
• Fuse specication: 250 V F1A, glass 5 x 20 mm
Note: The fuse may blow. In most cases, replacing the fuse with the correct type and rating will
resolve the issue. However, should you encounter a blown fuse frequently, please contact your
distributor for further assistance.
Accessories and spare parts
For current accessories and spares, please check our website coleparmer.com
Warranty
These microscopes come with a ve (5) year factory warranty against manufacturing defects, covering labor and
parts. Warranty does not apply for consumable accessories and parts such as bulbs, batteries, fuses, cords, optical
components, or any add-on accessories such as mechanical specimen holders that are not built into the microscope
stage as an integral part of the original manufacture, etc. Warranty does not cover microscopes, lenses cameras or
other accessories that have become inoperable due to dirt or damage due to misuse or lack of maintenance.
Note: Buyers are responsible for return shipping and handling cost for warranty services. Warranty covers parts and
labor only.
13

Notes
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Phone: [phone]
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1065Y62_M_CP_MSU-200_Series_Compound Microscope_Manual