Cole Parmer MSU-400 Series User manual
Installation and Operating Instructions
MSU-400 Series Compound Microscopes
Models 78904-02, -04, -07, -10, -13
78904-02
78904-07
78904-13
Introduction ____________________________________________________________________ 2
General safety instructions _______________________________________________________ 3
Dangers associated with the operation __________________________________________________________ 3
Photobiological safety LED, important safety instructions __________________________________________ 3
Prevention of biological and infectious hazards ___________________________________________________ 3
Disinfection and decontamination ______________________________________________________________ 4
Environment, storage and use __________________________________________________________________ 5
Models _________________________________________________________________________ 6
Objectives ______________________________________________________________________ 6
Components of the microscope ___________________________________________________ 7
Preparing the microscope for use__________________________________________________ 8
Assembling steps _____________________________________________________________________________ 8
Operation ______________________________________________________________________ 9
Setting up the illumination_____________________________________________________________________ 9
Placing the specimen slide _____________________________________________________________________ 9
Focusing and slide protection _________________________________________________________________ 10
Adjusting the focusing tension ________________________________________________________________ 10
Eyepieces___________________________________________________________________________________ 10
Abbe condenser _____________________________________________________________________________ 11
The eld (Köhler) diaphragm __________________________________________________________________ 11
Adjusting the aperture diaphragm _____________________________________________________________ 11
Use of the S100x oil-immersion objective________________________________________________________ 12
Illumination_________________________________________________________________________________ 12
Phase contrast (model 78904-10) ______________________________________________ 12-13
Supplementary manual for uorescence attachment (model 78904-13) ____________ 14-16
Maintenance and cleaning_______________________________________________________ 17
Cleaning the optics __________________________________________________________________________ 17
Maintenance of the stand _____________________________________________________________________ 17
Replacing the fuse ___________________________________________________________________________ 17
Using the Kensington™ security slot ____________________________________________________________ 17
Digital cameras ________________________________________________________________ 18
Accessories and spare parts______________________________________________________ 18
Warranty ______________________________________________________________________ 18
Notes _________________________________________________________________________ 19
Contents
Introduction
Thank you for purchasing a Cole-Parmer MSU-400 Series compound microscope. The MSU-400 series has been
designed with all kinds of Educational and Life Sciences applications and great durability in mind. This resulted
in a modern, robust and high-level microscope for advanced use, equipped with the best optical and mechanical
components. Specic attention to production methods resulted also in an excellent price/performance ratio
Please read this manual carefully before using this product to ensure correct and safe usage
• The content of this manual is subject to change without notice
• The appearance of the actual product can dier from the models described in this manual
• Not all equipment mentioned in this manual has to be part of the set you have purchased
• All optics are anti-fungus treated and anti-reection coated for maximum light throughput
2
General safety instructions
Intended use: A non-medical device
This microscope is intended for general observation of cells and tissues, with transmitted/reected illumination and
with the specimen xed on a slide.
Dangers associated with the operation
• Improper use could result in injury, malfunction or damage to property. It must be ensured that the operator
informs every user of existing hazards
• Danger of electrocution. Disconnect the power to the entire lighting system before installing, adding or
changing any component
• Not to be used in corrosive or explosive environments
• Avoid direct exposure of eyes to the collimated light beam or direct light from the light guides or bers
• To avoid a hazard to children, account for all parts and keep all packing materials in a safe place
Photobiological safety LED, important safety instructions
• Avoid direct eye exposure to any LED light source while switched on
• Before looking through the eyepieces of the microscope, lower the intensity of the LED illumination
• Avoid long and high-intensity exposure to LED light because this may cause acute damage to the retina of the eye
Prevention of biological and infectious hazards
Infectious, bacterial or viral biohazard substances under observation may be a risk to the health of humans and other
living organisms. Special precautions should be taken:
• Biological hazards: keep a logbook of all the biological substances or pathogenic microorganisms that were
under observation with the microscope and show it to everybody before they use the microscope or before
they do some maintenance work on the microscope! Agents can be bacterial, spores, enveloped or non-
enveloped virus particles, fungi or protozoa
• Contamination hazard:
• A sample that is properly enclosed with a cover glass never comes in direct contact with the microscope
parts. In that case prevention of contamination lies in the handling of the slides; as long as the slides
are decontaminated before use and are undamaged and treated normally, there is virtually zero risk of
contamination
• A sample that is mounted on a slide without cover glass, can come in contact with components of the
microscope and may be a hazard to humans and/or the environment. Therefore, check the microscope and
accessories on possible contaminations. Clean the microscope surfaces and its components as thoroughly
as possible. Should you identify a possible contamination, inform the local responsible person in your
organization
• Microscope operators could be contaminated from other activities and cross-contaminate components
of the microscope. Therefore, check the microscope and accessories on possible contaminations. Clean
the microscope surfaces and its components as thoroughly as possible. Should you identify a possible
contamination, inform the local responsible person in your organization. it is recommended to wear sterile
gloves when preparing the slides and handling the microscope in order to reduce contamination by the
operator
• Infection hazard: Direct contact with the focusing knobs, stage adjustments, stage and eyepieces/tubes of
the microscope can be a potential source of bacterial and/or viral infections. The risk can be limited by using
personal eyeshades or eyepieces. You can also use personal protections such as operation gloves and/or safety
goggles, which should be changed frequently to minimize the risk
• Disinfectant hazards: Before cleaning or disinfecting, check if the room is adequately ventilated. If not, wear
respiratory protective gear. Exposure to chemicals and aerosols can harm human eyes, skin and respiratory
system. Do not inhale vapours. During disinfection, do not eat, drink or smoke. Used disinfectants must be
disposed of according to local or national regulations for health and safety
3
Disinfection and decontamination
• Exterior casing and mechanical surfaces must be wiped with a clean cloth, dampened with a disinfectant
• Soft plastic parts and rubber surfaces can be cleaned by gently wiping a clean cloth, dampened with a
disinfectant. Discoloration can occur if alcohol is used
• The front lens of eyepieces and objectives are sensitive to chemicals. We recommend not to use aggressive
disinfectants but to use lens paper or a soft ber-free tissue, damped in cleaning solution. Cotton swabs may
also be used. We recommend you use personal eyepieces without eyeshades in order to minimize risk
• Never immerse or dip the eyepiece or objective into a disinfectant liquid! This will damage the component
• Never use abrasive compounds or cleaners that may damage and scratch optical coatings
• Properly clean and disinfect all possible contaminated surfaces of the microscope or contaminated accessories
before storing for future use. Disinfection procedures must be eective and appropriate
• Leave the disinfectant on the surface for the required exposure time, as specied by the manufacturer. If the
disinfectant evaporates before the full exposure time, reapply disinfectant on the surface
• Against bacteria, use a 70% aqueous solution of isopropanol (isopropyl alcohol) and apply for at least 30
seconds. Against viruses, we recommend to refer to specic alcohol or non-alcohol based disinfection products
for laboratories
Before returning a microscope for repair or maintenance through a Cole-Parmer dealer, an RMA (return authorization
form) together with a decontamination statement must be lled in! This document must be shipped together with the
microscope at all times
Handle with care
• This product is a high quality optical instrument. Delicate handling is required
• Avoid subjecting it to sudden shocks and impacts
• Impacts, even small ones, can aect the precision of the instrument
Handling the LED
Note: Always disconnect the power cord from your microscope before handling the LED bulb and power unit and
allow the system to cool down approximately 35 minutes to avoid burns
• Never touch the LED with your bare hands
• Dirt or ngerprints will reduce the life span and can result in uneven illumination, lowering the optical
performance
• Use only original MSU-400 Series replacement LEDs
• The use of other products may cause malfunctions and will void warranty
• During use of the microscope the power unit will get hot; never touch it while in operation and allow the system
to cool down approximately 35 minutes to avoid burns
Dirt on the lenses
• Dirt on or inside the optical components, such as eyepieces, lenses, etc., aects the image quality of your system
negatively
• Always try to prevent your microscope from getting dirty by using the dust cover, prevent leaving ngerprints
on the lenses and clean the outer surface of the lens regularly
• Cleaning optical components is a delicate matter. Please, read the cleaning instructions further on in this
manual
4
Environment, storage and use
• Maximum altitude: 2000 m
• Pollution degree: 2
• This product is a precision instrument and it should be used in a proper environment for optimal use
• Install your product indoors on a stable, vibration free and level surface in order to prevent this instrument to
fall thereby harming the operator
• Do not place the product in direct sunlight
• The ambient temperature should be between 5 to +40°C and humidity should be within 20 to 80% RH
• Although the system is anti-mold treated, installing this product in a hot, humid location may still result in the
formation of mold or condensation on lenses, impairing performance or causing malfunctions
• Never turn the right and left focus knobs in opposite directions at the same time or turn the coarse focus knob
past its farthest point as this will damage this product
• Never use undue force when turning the knobs
• Make sure that the microscope system can dissipate its heat (re hazard)
• Keep the microscope away from walls and obstructions for at least approximately 15 cm
• Never turn the microscope on when the dust cover is in place or when items are placed on the microscope
• Keep ammable uids, fabric, etc. well out of the way
Disconnect power
Always disconnect your microscope from power before doing any maintenance, cleaning, assembling or replacing
LEDs to prevent electric shocks
Prevent contact with water and other uids
Never allow water or other uids to come in contact with your microscope, this can cause short circuiting your device,
causing malfunction and damage to your system
Moving and assembling
• This microscope is a relatively heavy system, consider this when moving and installing the system
• Always lift the microscope by holding the main body and base of the microscope
• Never lift or move the microscope by its focusing knobs, stage or head. When needed, move the microscope
with two persons instead of one
5
Models
The Cole-Parmer MSU-400 Series compound microscopes are equipped with HWF 10x/20 mm eyepieces, and are
available in the following brighteld and phase contrast models. Model 78904-13 includes a uorescence attachment
with four 5 W LEDs for uorescence excitation from 450 to 470 nm (blue), 515 to 535 nm (green), 390 to 400 (violet) and
360 to 370 nm (ultraviolet; and a UV protection shield.
Please note: on coleparmer.com you can nd the latest updates about MSU-400 models and accessories
Objectives
Eyepiece
magnication
Objective
magnication
Total m
magnication
10x 4x 40x
10x 10x 100x
10x 20x 200x
10x 40x 400x
10x 60x 600x
10x 100x 1000x
The total magnication of the microscope can be calculated by multiplying the magnication of the eyepiece with the
magnication of the objective. The magnications are displayed in the table. The S40x, S60x and S100x objectives are
equipped with a spring mount, to prevent damage to the front lens and the slide
Model Type Objectives Working
distance Stand Illumination Power Weight
78904-02 Binocular 4x/10x/S40x/
S100x, semi-plan 48 to 76 mm Rackless integrated
X-Y mechanical stage
3 W adjustable Kohler
NeoLED
100–240 VAC,
50/60 Hz 14.3 lb (6.5 kg)
78904-04 Trinocular 4x/10x/S40x/
S100x, semi-plan 48 to 76 mm Rackless integrated
X-Y mechanical stage
3 W adjustable Kohler
NeoLED
100–240 VAC,
50/60 Hz 14.7 lb (6.6 kg)
78904-07 Trinocular 4x/10x/S40x/
S100x, plan 48 to 76 mm Rackless integrated
X-Y mechanical stage
3 W adjustable Kohler
NeoLED
100–240 VAC,
50/60 Hz 14.7 lb (6.6 kg)
78904 -10 Trinocular
10x/20x/S40x/
S100x, plan,
phase contrast
48 to 76 mm Rackless integrated
X-Y mechanical stage
3 W adjustable Kohler
NeoLED
100–240 VAC,
50/60 Hz 14.7 lb (6.6 kg)
78904-13 Trinocular 4x/10x/S40x/
S100x, plan 48 to 76 mm Rackless integrated
X-Y mechanical stage
3 W adjustable Kohler
NeoLED; four 5 W LED
Epi-illumination for
uorescence
100–240 VAC,
50/60 Hz 14.7 lb (6.6 kg)
6
A
B
C
E
D
F
G
H
P
O
R
Q
I
J
K
L
N
M
Components of the microscope
The names of the several component parts are listed below and are indicated in the picture:
A Camera focus adjustment ring J Diopter adjustment
BTrinocular tube KSlide for polarization lter
CMicroscope head LNosepiece
DTransport handle MObjectives
EHeight adjustment condenser N Stage with X-Y mechanical stage
FSlide protection handle O X-Y stage controls
G Coaxial coarse-and ne adjustment P Condenser with iris diaphragm
H Light intensity adjustment knob Q Köhler iris diaphragm
IEyepieces RCollector lens
7
A
B
A B C
Preparing the microscope for use
Carefully remove the items from its packaging and place them on a at, rm surface. Please
do not expose the microscope to direct sun light, high temperatures, damp, dust or acute
shake. Make sure the table or surface is at and horizontal
When moving the microscope, use the left hand to hold the transport handle (A) and
hold the base of the microscope with the right hand
Caution! Hold the microscope at the top of the stand arm when it is moved
Insert the power cord into the back of the microscope and use
the cable storage system (CSS) to store excessive cable while in
use or to store the cable after use (B)
Caution! If the bacterial solution or water splatters over the
stage, objective or head, pull out the power cord immediately
and dry the microscope
Assembling steps
Mounting the objectives
1. Rotate the coarse focusing knob to lower the stage to its
lowest position
2. Install the objectives into the objective nosepiece from
the lowest magnication to the highest in a clockwise direction from the rear side of the microscope. When
operating, start using the low magnication objective (4X or 10X) to search for specimen and focus, then
continue with high magnication objective to observe
The microscope head
The standard MSU-400 series conguration is supplied with the head assembled. However, if your order contains a
uorescence attachment (model 78904-13) then this should be mounted rst; see pages 14-16 for supplementary
manual.
Placing the eyepieces
The standard MSU-400 series conguration is supplied with the eyepieces assembled. However, if your order contains
additional eyepieces, please follow these steps to mount/replace them:
1. Unscrew the current eyepiece from the eyepiece tube with a screwdriver (see pictures A and B)
Note: The eyepiece tube with the diopter adjustment has three screws. In order to remove the eyepiece you need to
unscrew the one aligned with “0” (see picture C)
2. Insert the eyepiece into the eyepiece tube
3. Lock the eyepieces with a screwdriver
8
The eyeshades (optional)
The eyepieces can be equipped with optional rubber eyeshades. This prevents damage to the lens, and prevents stray
light. The eyeshade can simply be slipped over the eyepiece
Connecting the power cord
The MSU-400 microscopes support a wide range of operating voltages: from 100 to 240 VAC. Please use a grounded
power connection
• Make sure the power switch is o before connecting
• Insert the connector of the power cord into the power socket, and make sure it connects well
• Insert the other connector into the mains socket, and make sure it connects well. Do not bend or twist the
power cord, it will get damaged. Use the power cord that is supplied by Cole-Parmer. If it is lost or damaged,
choose one with the same specications
Replacement of the Power Cord
If the power cord requires replacement, ensure that it is suitably rated. If in doubt consult your local dealer or qualied
electrician
Operation
Setting up the illumination
For optimal contrast and resolution one should follow the below procedure:
1. Place a specimen on the object stage and focus using the 4x objective, with a fully opened iris diaphragm
2. Turn light intensity to lowest position, then look through the eyepiece(s) and turn up to the comfortable light
intensity level
3. Turn the condenser in the highest position (for phase contrast models, please set condenser to brighteld
position)
4. Close the iris diaphragm, until it is just visible on the edge of the eld of view
The microscope is properly set for use with the 4x objective. For each other magnication in brighteld use this
procedure should be repeated to ensure the best balance between contrast and resolution. Phase contrast set up is
explained later in this manual
Caution:
The maximum light intensity when using the 4x and 10x can damage the eyes!
Placing the specimen slide
1. Push the arm of the specimen holder backwards
2. Release the arm slowly clamping the slide with the cover glass facing up
3. Rotating the X and Y-axis knob will move the specimen to the center for alignment with the center of the
objective
9
Focusing and slide protection
1. Select the 4x objective and make sure that it is placed correctly in the optical path
2. Move the stage to the top by using the coarse adjustment knob and focus till the image appears
3. Rotate the ne focusing knob to sharpen the image
4. When you perform focusing with a S100x objective, you need to lock the slide protection handle. The slide
protection handle protects the slide by limiting the travel range of the mechanical stage. This way the objectives
will not touch or damage your slides
Adjusting the focusing tension
The tension of the focusing knobs can be adjusted. You can set it
from light to heavy according your own preference. Please note
that when the specimen leaves the focus plane after focusing
or the stage declines out of its own, then you need to adjust the
tension
To tighten the focusing knob (more heavy), rotate the tension
adjustment ring counter-clockwise; to loosen it, please turn it in
the clockwise direction
Eyepieces
Using a binocular (or trinocular) tube is less tiring for the eyes than
a monocular tube. In order to obtain a smooth “compound” image,
we recommend you to go through the below steps:
1. The interpupillary distance
The correct interpupillary distance is reached when one round image is seen in the eld of view (see image below).
This distance can be set by either pulling the tubes towards each other or pulling them away from each other. This
distance is dierent for each observer and thus should be set individually. When more users are working with the
microscope it is recommended to remember your interpupillary distance for a quick set-up during new microscopy
sessions. The MSU-400’s swiveling eyepiece tube can be rotated 360º.
You can select corresponding eye point height according to your own
preference
2. The correct eye point
The eye point is the distance from the eyepiece to the user’s pupil. To
obtain the correct eye point, move the eyes towards the eyepieces until
a sharp image is reached at a full eld of view
3. Adjusting the diopter
Using a binocular (or trinocular) tube is less tiring for the eyes than the
use of a monocular tube. In order to obtain the right interpupillary
setting, one should go through the below steps
• Set diopter adjustment ring to zero
• Close the left eye and focus the right tube by adjusting the
coarse- and ne adjustment knobs
• Close the right eye and focus the left tube with the diopter
adjustment ring
This procedure should be followed by each individual user. When
more users are working on the same microscope it is recommended
to remember your own diopter setting for a quick set-up during
new microscopy sessions
Focusing tension
Field of view
before adjustment
slide protection
Field of view
after adjustment
10
A
B
Abbe condenser
Beneath the object stage an Abbe condenser N.A. 1.25 is mounted. The condenser can be adjusted in height by
moving the rack and pinion knob beneath the mechanical stage. By adjusting the condenser you can focus the light on
the specimen for a optimized contrast. The condenser is factory pre-centered. If needed, the following procedure can
be followed to center the condenser
1. Move the condenser to its highest position
2. Select the 10x objective and place it into the light path and focus the specimen
3. Rotate the eld diaphragm adjustment ring to put the eld diaphragm to the smallest position
4. Adjust the condenser to the point where the image is the sharpest
5. Adjusting the center adjustment screw and put the image to the center of the eld of view
6. Open the eld diaphragm gradually
7. The condenser is centered correctly if the image remains in the center when you open the eld diaphragm and
inscribed to the eld of view
The eld (Köhler) diaphragm (see A in picture below)
By limiting the diameter of the beam entering the condenser, the eld diaphragm can prevent other light from
entering and increase the image contrast. When the image is just on the edge of the eld of view, the objective will
show the best performance and obtain the clearest image. The diaphragm is factory pre-centered.
Adjusting the aperture diaphragm (see B in picture below)
• The aperture diaphragm is used to select the numerical aperture (N.A.) of the illumination. When the N.A. of
illumination matches with the N.A. of the objective, you get the highest possible resolution, depth of eld and
contrastare obtained
• When contrast is low, rotate the diaphragm adjustment ring to 70%-80% of the N.A. of objective this will
improve the contrast of the image. The diaphragm is factory pre-centered
11
Use of the S100x oil-immersion objective
The MSU-400 Series microscopes are equipped with an S100x N.A. 1.25 oil immersion objective. Please follow the
below instructions on how to use this objective:
1. Remove the dust protection cap from the revolving nosepiece to mount the S100x objective
2. Focus the image with the S40x objective
3. Lock the slide protection handle
4. Turn the revolving nosepiece so the S100x objective almost reaches the click-stop
5. Put a small drop of immersion oil on the center of the slide
6. Now turn the S100x objective so that you feel the click stop
7. The front lens is in contact with the immersion oil
8. Look through the eyepiece and focus the image with the ne adjustment knobs
9. The distance between the lens of the objective and the slide is very small!
10. In case there are small bubbles visible, turn the S100x objective a couple of times from left to right so that the
front of the objective moves in the oil and the bubbles will disappear
11. After using the S100x objective, loosen the slide protection handle and turn the table with the course
adjustment knobs downwards until the front lens doesn’t touch the oil any longer. Clean the front lens of the
S100x objective
12. Always clean the front lens of the S100x objective with a piece of lens paper that is moistened with a drop of
isopropanol.
13. Clean the slide after use as well
Illumination
The illumination has the following specications:
LED : 3W NeoLED for binocular and trinocular models.
Power supply : Primary 100-240 VAC, 50/60 Hz
Fuse specication : 250V 1A
Phase contrast (model 78904-10)
Use of phase contrast
The phase contrast method was designed in 1934 by the Dutchman Frits Zernike to observe very thin or transparent
objects. This technique uses the fact that light travelling through tissue undergoes a phase shift due to diraction. By
recombining the phase shifted light with the background light, a contrasted image appears in the eyepiece.
Using the Zernike phase contrast set
Any MSU-400 model with a Zernike phase contrast set comes with the phase contrast condenser and objectives
already mounted and centered on your microscope. If you suspect misalignment or want to check the alignment
please see the next point for “centering the phase rings”. The height of the condenser can be adjusted by turning
the rack and pinion up and down. By doing this the light beam will be focused more on the specimen for maximum
resolution.
12
C
B
A
Centering the phase rings
The Zernike phase disc has ve positions: 0 for brighteld observation (this position also has an iris diaphragm), 10, 20,
40, and 100.
These positions correspond to the respective phase contrast objectives 10x, 20x, 40x and 100x.
When the condenser is in the “0” position, the objectives can be used for brighteld observation. For phase contrast,
the condenser position should match the objective used, meaning that when the condenser is in position “40” the
objective used should also be 40x.
•
Rotate the 10× innity phase contrast objective into the eld of view, then set the Zernike phase disc to position
“10” to match the objective
•
Take the eyepiece out of the tube and insert centering telescope in its place. When looking through the
centering telescope, the dark and bright ring images should coincide with each other as shown in the pictures
below. If the ring images cannot be observed clearly, focus the centering telescope rst (A) and if this does not
solve the issue then try to adjust the condenser by turning it up and down (B).
•
If the bright ring and dark ring images do not coincide as shown below, adjust the position of the ring by
moving the ring at the bottom of the condenser with the centering knobs (C). Move it until the bright and dark
ring images superimpose. Repeat for all objectives/Zernike disc positions
15
These positions correspond to the respective phase contrast objectives 10x, 20x, 40x and 100x.
When the condenser is in the “0” position, the objectives can be used for brightfield observation. For phase
contrast, the condenser position should match the objective used, meaning that when the condenser is in
position “40” the objective used should also be 40x
Rotate the 10× infinity phase contrast objective into the field of view, then set the Zernike phase disc to position
“10” to match the objective
Take the eyepiece out of the tube and insert centering telescope in its place. When looking through the centering
telescope, the dark and bright ring images should coincide with each other as shown in the pictures below. If the
ring images cannot be observed clearly, focus the centering telescope first (A) and if this does not solve the issue
then try to adjust the condenser by turning it up and down (B).
If the bright ring and dark ring images do not coincide as shown below, adjust the position of the ring by moving
the ring at the bottom of the condenser with the centering knobs (C). Move it until the bright and dark ring
images superimpose. Repeat for all objectives/Zernike disc positions
Not centered Centered properly
Not centered
15
These positions correspond to the respective phase contrast objectives 10x, 20x, 40x and 100x.
When the condenser is in the “0” position, the objectives can be used for brightfield observation. For phase
contrast, the condenser position should match the objective used, meaning that when the condenser is in
position “40” the objective used should also be 40x
Rotate the 10× infinity phase contrast objective into the field of view, then set the Zernike phase disc to position
“10” to match the objective
Take the eyepiece out of the tube and insert centering telescope in its place. When looking through the centering
telescope, the dark and bright ring images should coincide with each other as shown in the pictures below. If the
ring images cannot be observed clearly, focus the centering telescope first (A) and if this does not solve the issue
then try to adjust the condenser by turning it up and down (B).
If the bright ring and dark ring images do not coincide as shown below, adjust the position of the ring by moving
the ring at the bottom of the condenser with the centering knobs (C). Move it until the bright and dark ring
images superimpose. Repeat for all objectives/Zernike disc positions
Not centered Centered properly
Centered properly
13
Supplementary manual for uorescence attachment (model 78904-13)
Installation of the uorescence illuminator and microscope head
Most parts are already mounted on the microscope. Only two parts are packed separately. The microscope head (B)
and the uorescence illuminator (F).
A. Eyepieces
B. Microscope head
C. Fluorescence attachment
D. Turret for uorescence lters
E. Protection screen
F. Fluorescence illuminator
G. Turret for LEDs
Installation steps
• Take the microscope with uorescence attachment, uorescence illuminator and head from its packaging
• Place the head on the uorescence attachment and fasten the screw (on the right side of the microscope) with a
hexagon wrench
• Install the eyepieces into the eyepiece tubes if these are not already installed (g 1, A)
• Mount the uorescence illuminator to the back of the uorescence attachment and fasten the screw with a
hexagon wrench
• Plug the power cord into the power supply, but don’t turn it on yet
1
14
Operation of reected illumination system (LED uorescence unit)
To get the best result when observing with uorescence please pay attention to the following:
1. Select the uorescence lter that matches the uorochrome and uorescent characteristic
2. No autouorescence specimen or device should be in the optical system, such as the cedar immersion liquid
which has cyan autouorescence
3. Always turn o the transmitted illumination before turning on the reected illumination system
Set and adjust the uorescence unit
The most important part of the unit is the uorescence lters assembly. There are four uorescence lters, Blue, Green,
Violet and Ultraviolet, mounted in the turret (g. 1, D) and are indicated as B, G, V and UV on the turret. There is also a
brighteld option and it is indicated as 0 on the turret.
The uorescence lters in the turret correspond with the LEDs in the uorescence illuminator (see table). There is a
turret (g. 1, G) on top of the uorescence illuminator and has the same indication of B, G, V and UV as the turret for the
uorescence lters.
Fluorescence lters LED light source
B B
G G
V V
UV UV
When selecting the lter, turn the turret (D) to the correct lter and choose the corresponding LED by turning the
turret on the illuminator (G).
Remark: For brighteld observation please choose position 0 on the turret (D) and switch o the
uorescence illuminator and turn on the transmitted illumination
15
Operation of the eld diaphragm
The eld diaphragm has been pre-centered and is ready to use, but due to possible vibration or inclination during
transport it may deviate, therefore always double check.
If it is not pre-centered, then please follow the following steps:
1. Use objective 10x
2. Push in the eld diaphragm switch (the one in the front, (g.2, A), a light spot will be visible in the eld of view
3. Adjust the centering if the light spot deviates from the center of the view of view (g. 3, a) by using the
adjustment screws behind the eld diaphragm switch (g. 2, B). The light spot should be centered as shown in
g. 3, b
4. Pull out the switch so that the image lls the eld of view as shown in g. 3, c
2
3
abc
16
17
RReeppllaacciinngg tthhee ffuussee
To change the fuse, please follow these procedures:
• Remove the power cord from the back of the microscope
• Locate the fuse compartment, which has a fuse image. It is typically located beneath
the power connector
• Remove the fuse compartment. To do so, insert a flat head screw driver between the
metal power tines and gently pry the fuse compartment loose with a slight downward
and outward motion
• Insert the new fuse into the compartment, and replace the fuse compartment cover
to where it originally was
• Power up the microscope and test
NNoottee::
The fuse may blow. In most cases, replacing the fuse with the correct voltage will
resolve the issue. However, should you encounter a blown fuse frequently, please contact your distributor for
further assistance
FFuussee ssppeecciiffiiccaattiioonn:: 225500VV,, 115500 mmAA
RReeppllaacciinngg//ppllaacciinngg tthhee rreecchhaarrggeeaabbllee bbaatttteerriieess ((ooppttiioonnaall))
1. Remove the power cord from the back of the microscope
2. Place the microscope on its back
3. Remove the six screws of the base of the microscope
Location of screws are indicated on drawing aside
The battery compartment in located on the baseplate
4. Open battery compartment, by removing the small screw
on top, slide compartment open
5. Place batteries and close the compartment
Note: Always use high quality rechargeable batteries, preferably supplied by Euromex. Minimum 1800mA
capacity type. Charge batteries fully for 8 hours. Use the microscope untill batteries are fully depleted then
recharge again. Average use with full batteries: 8-32 hours, depending on light intensity and battery capacity and
quality
Green battery indicator: batteries are charged
Red battery indicator: batteries are being charged
Maintenance and cleaning
Always place the dust cover over your microscope after use. Always keep the eyepiece and objectives mounted on the
microscope to avoid dust entering the instrument
Cleaning the optics
When the eyepiece lens or front lens of the 10x or S40x objective are dirty, they can be cleaned by wiping a piece of
lens paper over the surface (circular movements). When this does not help put a drop of alcohol on the lens paper and
wipe it. Never put xylol or alcohol directly on the lens!
It is not necessary – and not recommended – to clean the lens surfaces at the inner side of the objectives. Sometimes
dust can be removed with high pressured air. There will never be dust in the objectives if the objectives are not
removed from the revolving nosepiece
Caution
Cleaning cloths containing plastic bers can damage the coating of the lenses!
Maintenance of the stand
Dust can be removed with a brush. In case the stand or table is really dirty then you can clean the surface with a non-
aggressive cleaning product. All moving parts like the height adjustment or the coaxial course and ne adjustment
contain ball bearings that are not dust sensitive. With a drop of sewing-machine oil you can lubricate the bearing can
be lubricated
Replacing the fuse
To change the fuse, please follow these procedures below:
• Remove the power cord from the back of the microscope
• Locate the fuse compartment, which has a fuse image. It is typically located beneath the
power connector
• Remove the fuse compartment. To do so, insert a at head screw driver in between the
metal power tines and gently pry the fuse compartment loose with a slight downward
and outward motion
• Insert the new fuse into the compartment, and then replace the fuse compartment cover
to where it originally was
• Power up the microscope and test
• Fuse specication: 250V, 150 mA
Note:The fuse may blow.in order to prevent internal damage to the microscope. And iIn most cases, replacing the
fuse with a correctly rated fuse will resolve the issue. However, should you encounter a blown fuse frequently, please
contact your distributor for further assistance
Using the Kensington™ security slot
At the backside of the microscope a Kensington™ security slot is placed, which can be used to secure the instrument
from theft using a Kensington™ lock (not supplied)
17
A
Digital cameras
For trinocular models, slide the camera with mounted C-mount adapter into
the 23,2mm tube of the photo port. Take an easy-to-view specimen and focus
the image through the microscope’s eyepieces. For focusing the camera, slowly
move tube (A) up and down while watching at the screen untill the camera view
is in focus
Follow the manual that comes with the camera for camera operation
Accessories and spare parts
For current accessories and spares, please visit our
website coleparmer.com
Warranty
These microscopes come with a ve (5) year factory warranty against manufacturing defects, covering labor and
parts. Warranty does not apply for consumable accessories and parts such as bulbs, batteries, fuses, cords, optical
components, or any add-on accessories such as mechanical specimen holders that are not built into the microscope
stage as an integral part of the original manufacture, etc. Warranty does not cover microscopes, lenses cameras or
other accessories that have become inoperable due to dirt or damage due to misuse or lack of maintenance.
Note: Buyers are responsible for return shipping and handling cost for warranty services. Warranty covers parts and
labor only.
Trinocular MSU-400 head
with camera in photo port
18
Notes
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©2022 Cole-Parmer Instrument Company, LLC.
Toll-free: 1-800-323-4340
Phone: 1-847-549-7600
Fax: 1-847-247-2929
coleparmer.com
1065Y62_M_CP_MSU-400_Series_Compound Microscopes_Manual
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