Cole Parmer MSS-200 Series User manual

Installation and Operating Instructions
MSS-200 Series Trinocular
Stereozoom Microscopes
Models 78904-66, -69
78904-66
78904-69

Contents
1. Introduction
With your purchase of a Cole-Parmer MSS-200 Series microscope you have chosen a quality product. The MSS-200
Series stereomicroscopes are developed for professional industrial purposes, scientic research and education
because of the excellent optical and mechanical properties, while very attractively priced.
The stereomicroscope consists of two separate microscope tubes which are combined as a unit, in order to focus
them simultaneously on the object. Each tube has prisms, plan achromatic objectives and a wideeld eyepiece in
order to obtain a large, at eld of view. Both eyes are looking at the object under a dierent angle to reach a deep
stereoscopic image. The maintenance requirement is minimal when using the microscope in a proper manner.
This manual describes the construction of the microscope, how to use the microscope and its maintenance.
1. Introduction _________________________________________________________________ 2
2. General safety instructions ____________________________________________________ 3
2.1 Dangers associated with the operation ______________________________________________________ 3
2.2 Photobiological safety LED, important safety instructions _____________________________________ 3
2.3 Prevention of biological and infectious hazards ______________________________________________ 3
2.4 Disinfection and decontamination: _________________________________________________________ 4
2.5 Environment, storage and use ______________________________________________________________ 5
3. Models _____________________________________________________________________ 5
4. Components of the stereomicroscope __________________________________________ 6
4.1 Eyepieces________________________________________________________________________________ 7
4.2 Head ___________________________________________________________________________________ 7
4.3 Objectives _______________________________________________________________________________ 7
4.4 Object stage _____________________________________________________________________________ 7
4.5 Focusing ________________________________________________________________________________ 7
4.6 Magnication adjustment__________________________________________________________________ 7
4.7 Illumination______________________________________________________________________________ 8
5. Preparing the stereomicroscope for use _________________________________________ 8
6. Assembling steps ____________________________________________________________ 8
6.1 Mounting the head________________________________________________________________________ 8
6.2 Placing the eyepieces _____________________________________________________________________ 8
6.3 Connecting the microscope ________________________________________________________________ 8
7. Working with the stereomicroscope ____________________________________________8
7.1 The interpupillary distance _________________________________________________________________ 8
7.2 The correct eyepoint ______________________________________________________________________ 8
8. How to set up a stereomicroscope ______________________________________________ 9
9. Photo and video _____________________________________________________________10
9.1 Trinocular models________________________________________________________________________ 10
10. Maintenance and cleaning __________________________________________________ 11
10.1 Cleaning the optics _____________________________________________________________________ 11
10.2 Maintenance of the stand _______________________________________________________________ 11
10.3 Replacing the fuse ______________________________________________________________________ 11
11. Accessories and spare parts __________________________________________________ 11
12. Warranty __________________________________________________________________ 11
13. Notes _____________________________________________________________________12
2

2. General safety instructions
Intended use: A non-medical device
This microscope is intended for general observation of cells and tissues, with transmitted/reected illumination and
with the specimen xed on a slide.
2.1 Dangers associated with the operation
• Improper use could result in injury, malfunction or damage to property. It must be ensured that the operator
informs every user of existing hazards
• Danger of electrocution. Disconnect the power to the entire lighting system before installing, adding or
changing any component
• Not to be used in corrosive or explosive environments
• Avoid direct exposure of eyes to the collimated light beam or direct light from the light guides or bers
• To avoid a hazard to children, account for all parts and keep all packing materials in a safe place
2.2 Photobiological safety LED, important safety instructions
• Avoid direct eye exposure to any LED light source while switched on
• Before looking through the eyepieces of the microscope, lower the intensity of the LED illumination
• Avoid long and high-intensity exposure to LED light because this may cause acute damage to the retina of the eye
2.3 Prevention of biological and infectious hazards
Infectious, bacterial or viral biohazard substances under observation may be a risk to the health of humans and other
living organisms. Special precautions should be taken during in vitro medical procedures:
• Biological hazards: Keep a logbook of all the biological substances or pathogenic microorganisms that were
under observation with the microscope and show it to everybody before they use the microscope or before
they do some maintenance work on the microscope! Agents can be bacterial, spores, enveloped or non-
enveloped virus particles, fungi or protozoa
• Contamination hazard:
• A sample that is properly enclosed with a cover glass never comes in direct contact with the microscope
parts. In that case prevention of contamination lies in the handling of the slides; as long as the slides
are decontaminated before use and are undamaged and treated normally, there is virtually zero risk of
contamination
• A sample that is mounted on a slide without cover glass, can come in contact with components of the
microscope and may be a hazard to humans and/or the environment. Therefore, check the microscope and
accessories on possible contaminations. Clean the microscope surfaces and its components as thoroughly
as possible. Should you identify a possible contamination, inform the local responsible person in your
organization
• Microscope operators could be contaminated from other activities and cross-contaminate components
of the microscope. Therefore, check the microscope and accessories on possible contaminations. Clean
the microscope surfaces and its components as thoroughly as possible. Should you identify a possible
contamination, inform the local responsible person in your organization. it is recommended to wear sterile
gloves when preparing the slides and handling the microscope in order to reduce contamination by the
operator
• Infection hazard: Direct contact with the focusing knobs, stage adjustments, stage and eyepieces/tubes of
the microscope can be a potential source of bacterial and/or viral infections. The risk can be limited by using
personal eyeshades or eyepieces. You can also use personal protections such as operation gloves and/or safety
goggles, which should be changed frequently to minimize the risk
• Disinfectant hazards: Before cleaning or disinfecting, check if the room is adequately ventilated. If not, wear
respiratory protective gear. Exposure to chemicals and aerosols can harm human eyes, skin and respiratory
system. Do not inhale vapours. During disinfection, do not eat, drink or smoke. Used disinfectants must be
disposed of according to local or national regulations for health and safety
3

2.4 Disinfection and decontamination
• Exterior casing and mechanical surfaces must be wiped with a clean cloth, dampened with a disinfectant
• Soft plastic parts and rubber surfaces can be cleaned by gently wiping a clean cloth, dampened with a
disinfectant. Discoloration can occur if alcohol is used
• The front lens of eyepieces and objectives are sensitive to chemicals. We recommend not to use aggressive
disinfectants but to use lens paper or a soft ber-free tissue, damped in cleaning solution. Cotton swabs may
also be used. We recommend you use personal eyepieces without eyeshades in order to minimize risk
• Never immerse or dip the eyepiece or objective into a disinfectant liquid! This will damage the component
• Never use abrasive compounds or cleaners that may damage and scratch optical coatings
• Properly clean and disinfect all possible contaminated surfaces of the microscope or contaminated accessories
before storing for future use. Disinfection procedures must be eective and appropriate
• Leave the disinfectant on the surface for the required exposure time, as specied by the manufacturer. If the
disinfectant evaporates before the full exposure time, reapply disinfectant on the surface
• Against bacteria, use a 70% aqueous solution of isopropanol (isopropyl alcohol) and apply for at least
30 seconds. Against viruses, we recommend to refer to specic alcohol or non-alcohol based disinfection
products for laboratories
Before returning a microscope for repair or maintenance through a Cole-Parmer dealer, an RMA (return authorization
form) together with a decontamination statement must be lled in! This document must be shipped together with the
microscope at all times.
Handle with care
• This product is a high quality optical instrument. Delicate handling is required
• Avoid subjecting it to sudden shocks and impacts
• Impacts, even small ones, can aect the precision of the instrument
Handling the LED
Note: Always disconnect the power cord from your microscope before handling the LED bulb and power unit and
allow the system to cool down approximately 35 minutes to avoid burns
• Never touch the LED with your bare hands
• Dirt or ngerprints will reduce the life span and can result in uneven illumination, lowering the optical
performance
• Use only original MSS-200 Series replacement LEDs
• The use of other products may cause malfunctions and will void warranty
• During use of the microscope the power unit will get hot; never touch it while in operation and allow the system
to cool down approximately 35 minutes to avoid burns
Dirt on the lenses
• Dirt on or inside the optical components, such as eyepieces, lenses, etc., aects the image quality of your system
negatively
• Always try to prevent your microscope from getting dirty by using the dust cover, prevent leaving ngerprints
on the lenses and clean the outer surface of the lens regularly
• Cleaning optical components is a delicate matter. Please, read the cleaning instructions further on in this
manual
4

2.5 Environment, storage and use
• Maximum altitude: 2000 m
• Pollution degree: 2
• This product is a precision instrument and it should be used in a proper environment for optimal use
• Install your product indoors on a stable, vibration free and level surface in order to prevent this instrument to
fall thereby harming the operator
• Do not place the product in direct sunlight
• The ambient temperature should be between 5 to +40°C and humidity should be within 20 to 80% RH
• Although the system is anti-mold treated, installing this product in a hot, humid location may still result in the
formation of mold or condensation on lenses, impairing performance or causing malfunctions
• Never turn the right and left focus knobs in opposite directions at the same time or turn the coarse focus knob
past its farthest point as this will damage this product
• Never use undue force when turning the knobs
• Make sure that the microscope system can dissipate its heat (re hazard)
• Keep the microscope away from walls and obstructions for at least approximately 15 cm
• Never turn the microscope on when the dust cover is in place or when items are placed on the microscope
• Keep ammable uids, fabric, etc. well out of the way
Disconnect power
Always disconnect your microscope from power before doing any maintenance, cleaning, assembling or replacing
LEDs to prevent electric shocks
Prevent contact with water and other uids
Never allow water or other uids to come in contact with your microscope, this can cause short circuiting your device,
causing malfunction and damage to your system
Moving and assembling
• This microscope is a relatively heavy system, consider this when moving and installing the system
• Always lift the microscope by holding the main body and base of the microscope
• Never lift or move the microscope by its focusing knobs, stage or head
• When needed, move the microscope with two persons instead of one
3. Models
The MSS-200 Series is available in two dierent models, with a choice of stand. They are equipped with 45° inclined
tubes and a zoom system with a pair of wideeld WF 10x/21 eyepieces.
Model Type Magnication Working distance Stand Illumination Power Weight
78904-66 Trinocular 0.7 to 4.5x 100 mm Pillar Incident (3 W LED);
transmitted (3 W LED)
100–240 VAC,
50/60 Hz 10.4 lb (4.2 kg)
78904-69 Trinocular 0.7 to 4.5x 100 mm Rack & pinion Incident (3 W LED);
transmitted (3 W LED)
100–240 VAC,
50/60 Hz 7.3 lb (3.3 kg)
5

A
B
E
D
F
C
G
H
H
L
J
M
K
N
O
P
Q
4. Components of the stereomicroscope
The names of the individual parts are listed below and are indicated in the picture:
A. Eyepiece/s
B. Diopter adjustment
C. Zoom adjustment knobs
D. Fixation screw
E. Objective
F. Brightness adjustment for transmitted
illumination
G. Object plate with transmitted illumination
below it
H. Stand base
I. Handle
J. Photo port
K. Microscope head
L. Stand (rack & pinion version)
M. Focusing knobs (only one is visible)
N. Incident illumination
O. Brightness adjustment for incident illumination/
ring illumination (if applicable)
P. Object clips
Q. On/O switch
The stereomicroscope consists of a stand with headholder/focusing system, in which the stereo head is placed and
locked with a xation screw (D).
6

4.1 Eyepieces
The standard MSS-200 Series is supplied with a pair of HWF 10x/21 mm eyepieces (A).
4.2 Head
Trinocular heads with 45° inclined tubes, which are rotatable over 360°. One or two eyepieces have a ± 5 diopter
adjustment (B), depending on the model. Interpupillary distance adjustable between 55 mm and 75 mm. Trinocular
head is supplied with a xed light path beam splitter (50:50).
4.3 Objectives
The standard MSS-200 Series is supplied with a 1:6.4 zoom objective with 0.7x to 4.5x magnications, a eld of view
from 29.9 mm to 4.6 mm. Working distance 100 mm.
4.4 Object stage
The rack & pinion stands and pillar stands are equipped with an object stage with a semi-transparent stage plate (G)
and two object clips (N).
4.5 Focusing
With the two coarse focusing knobs (M) the object can be sharply focused.
4.6 Magnication adjustment
• The microscopes are equipped with two plan achromatic zoom objectives. By using the zoom knobs (C) the
magnication can be adjusted in a smooth, stepless way.
4.7 Illumination
The rack & pinion stands and pillar stands are equipped with transmitted and incident 3 W LED illumination. The
illuminators can be used simultaneously, and their brightness can be adjusted using (F) and (O) knobs.
Eyepieces Zoom indication Magnication
10x 0.7x 7x
10x 0.8x 8x
10x 1x 10x
10x 1.5x 15x
Eyepieces Zoom indication Magnication
10x 2x 20x
10x 3x 30x
10x 4x 40x
10x 4.5x 45x
7

5. Preparing the stereomicroscope for use
Carefully remove the items from its packaging and place them on a at, rm surface. Please do not expose the
stereomicroscope to direct sun light, high temperatures, damp, dust or acute shaking. Make sure the table or surface is
at and horizontal. When moving the stereomicroscope, use one hand to hold the pillar or column and the other hand
to hold the base
Caution! Holding the stereomicroscope by its focusing knobs
will damage it
Caution! If the bacterial solution or water splatters over the stage plate, objective or head, pull out the
power cord immediately and dry the microscope
6. Assembling steps
6.1 Mounting the head
Remove the microscope head from its packaging and mount it on the microscope head holder. To do so, loose the
head xation screw (D) so that the head can be inserted all the way into the head holder. Once the head is in place,
tighten the head xation screw again. The photo tube of the trinocular versions is packed in the lid of the foam
packing. Take it out and screw it into the photo tube port (J)
6.2 Placing the eyepieces
Unpack the two eyepieces (A) and remove their dust covers. Then
remove the dust covers of the eyepiece tubes and insert the two
eyepieces into the tubes (the eyepieces should be pushed all the
way down, touching the tubes). The eyepieces can be secured with
a screw. The exact location of the hexagon screw may dier. Each
eyepiece has a rubber eyeshade. This prevents damage to the lens
and stray light. The eyeshade can simply be slipped over the eyepiece
6.3 Connecting the microscope
Connect the power cable to the mains supply and switch on the
microscope using the On/O switch (Q). Do not bend or twist the power cord as damage may occur. Use the power
cord supplied with the unit. If the cord requires replacement, ensure that it is suitably rated. If in doubt consult your
local dealer or qualied electrician.
7. Working with the stereomicroscope
7.1 The interpupillary distance
In order to obtain a smooth “compound” image, one should go through the steps below:
The correct interpupillary distance is reached when one round image is seen in the eld
of view (see image below). This distance can be set by either pulling the tubes towards
each other or pulling them from each other. This distance is dierent for each observer
and thus should be set individually. If several users are working with the microscope it is
recommended to remember your interpupillary distance for a quick setup during new
microscopy sessions
7.2 The correct eyepoint
The eyepoint is the distance from the eyepiece to the user’s pupil. To obtain the
correct eyepoint, move the eyes towards the eyepieces until a sharp image is
reached at a full eld of view Field of
view before
adjustment
Field of
view after
adjustment
8

8. How to set up a stereomicroscope
Setting up your stereomicroscope properly is essential to getting parfocal images during the entire zoom range.
It also prevents headaches, stressful eyes and fatigue. Below you will nd a setup guide to get the best out of your
microscope
Turn the diopter adjustment rings
of both eyepieces to position “0”
Put a specimen on the stage plate Turn the zoom adjustment knob to
the lowest magnication
Rotate the focusing knob
to bring the specimen into focus
Turn the zoom adjustment knob
to the highest magnication
Rotate the focusing knob to
bring the specimen into focus
Turn the zoom adjustment knob
to the lowest magnication
Rotate the diopter adjustment rings of
the left and right eyepiece to bring the
specimen into focus
Please note:
Put the zoom adjustment knob
at the highest magnication again
and check the image focusing. The
diopter adjustment is complete
when the image is accurately
focused during zooming. If not,
please repeat steps 3 to 8
9

D
C
A
B
9. Photo and video
9.1 Trinocular models
• For trinocular models using the standard supplied trinocular tube, slide the camera with mounted C-mount
adapter into the 23.2 mm tube of the photo port. For focusing, loosen the ring (A) and slowly unscrew the tube
(B) you will be able to match parfocality of the camera with the view through the eyepieces. Adjustment can
be made by raising/lowering the height of the camera (C). Take an easy-to-view specimen and focus the image
through the microscope’s eyepieces (with dioptre adjustment set on “0”). Afterwards, perform this height
adjustment procedure while watching the image on the computer screen. In this case, once you have obtained
parfocality in the device, screw the ring (A) back
• When using an optional C-mount adapter, turn ring (D) to focus
Follow the manual that comes with the camera for camera operation
10

10. Maintenance and cleaning
Always place the dust cover over your MSS-200 Series microscope after use. Always keep the eyepieces mounted on
the microscope to avoid dust entering the instrument
10.1 Cleaning the optics
When the eyepiece lenses are dirty, they can be cleaned by wiping a piece of lens paper over the surface (circular
movements). If this does not help put a drop of alcohol on the lens paper and wipe it. Never put Isopropyl or alcohol
directly on the lens! Cole-Parmer has various optical cleaning wipes available
It is not necessary - and not recommended - to clean the lens surfaces at the inner side of the objectives. Sometimes
dust can be removed with high pressured air
Caution Cleaning cloths containing plastic bers may damage the coating of the lenses!
10.2 Maintenance of the stand
• Dust can be removed with a brush. In case the stand or base plate is really dirty, then clean the surface with a
non-aggressive cleaning product
• All moving parts - like the height adjustment or the coaxial coarse and ne adjustment - contain ball bearings
that are not dust sensitive. With a drop of sewing-machine oil the bearing can be lubricated
10.3 Replacing the fuse
To change the fuse, follow the procedures below:
• Remove the power cord from the back of the microscope
• Locate the fuse compartment, which has a fuse image. It is typically
located close to the power connector
• Remove the fuse compartment. To do so, insert a at head
screwdriver behind the fuse compartment and gently pry it loose
• Insert the new fuse into the compartment, and then replace the
fuse compartment back to where it was originally
• Power up the microscope and test
• Fuse specication: 250 V F1A glass, 5 x 20 mm
Note: The fuse may blow in order to prevent internal damage to the microscope and in most cases, replacing the
fuse with a correctly rated fuse will resolve the issue. However, should you encounter a blown fuse frequently, please
contact your distributor for further assistance
11. Accessories and spare parts
For current accessories and spares, please check our website coleparmer.com
12. Warranty
These microscopes come with a ve (5) year factory warranty against manufacturing defects, covering labor and
parts. Warranty does not apply for consumable accessories and parts such as bulbs, batteries, fuses, cords, optical
components, or any add-on accessories such as mechanical specimen holders that are not built into the microscope
stage as an integral part of the original manufacture, etc. Warranty does not cover microscopes, lenses cameras or
other accessories that have become inoperable due to dirt or damage due to misuse or lack of maintenance.
Note: Buyers are responsible for return shipping and handling cost for warranty services. Warranty covers parts and
labor only.
11

13. Notes
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©2022 Cole-Parmer Instrument Company, LLC.
Toll-free: 1-800-323-4340
Phone: [phone]
Fax: [phone]
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1065Y62_M_CP_MSS-200_Series_Stereozoom Microscopes_Manual