Crystal AEOLUS IS-18CA User manual

AEOLUS™ Stackable
Incubator Shaker

Note
Please read this manual carefully before installing the equipment. Please follow all
the instructions contained in this manual during operation. Otherwise, the user shall
be responsible for any consequence arising therefrom including potential bodily
harm and/or property damage.
Copyright:
1. No part of this manual may be reproduced, distributed, or transmitted in any form
or by any means, including photocopying, recording, or other electronic or
mechanical methods, without the prior written permission of the publisher, Crystal
Technology & Industries, Inc.
2. Contents contained in this manual, including all technical data, specification, and
performance figures are subject to change without notice.
Preface
Thank you for choosing Crystal Technology & Industries, Inc. AEOLUS™ CO2 Incubator
Shaker. Your trust and support are greatly appreciated.
Our Incubator Shaker Series is designed for shaking and heating samples with optional
CO2 and humidity control to meet the research and experiment needs in modern
biotechnology. Our state-of-the-art embedded microprocessor system utilized in this
machine has powerful data processing ability, outstanding stability, excellent
interference resistance, and high precision control of the temperature and shaking
performance of the instrument. Sophisticated manufacturing processes, aesthetic product
design, and a user-friendly interface are elegantly incorporated into the production of the
instrument.
The Incubator Shaker is widely used in biology, microbiology, medicine, pharmaceutics,
food science, and environmental science, which include various biological and chemical
reactions that require high precision control of temperature and shaking. Applicable
processes include bacteria culture, fermentation, and hybridization, and enzyme and cell
tissue research. It is also applicable to both static and dynamic culture of microbial cells
and strains.
Table of Contents
1. Overview
2. Unpacking Guide
3. Preparation
4. Safety Instructions
5. Installation
6. Structure
7. Control Panel
8. Settings
9. Operating Instructions
10. Maintenance
11. Troubleshooting
12. Method of Use Accessories
13. Optional Accessories
14. Warranty
P9
P13
P14
P18
P19
P20
P31
P32
P33
P35
P43
P45
P7

Note
Please read this manual carefully before installing the equipment. Please follow all
the instructions contained in this manual during operation. Otherwise, the user shall
be responsible for any consequence arising therefrom including potential bodily
harm and/or property damage.
Copyright:
1. No part of this manual may be reproduced, distributed, or transmitted in any form
or by any means, including photocopying, recording, or other electronic or
mechanical methods, without the prior written permission of the publisher, Crystal
Technology & Industries, Inc.
2. Contents contained in this manual, including all technical data, specification, and
performance figures are subject to change without notice.
Preface
Thank you for choosing Crystal Technology & Industries, Inc. AEOLUS™ CO2 Incubator
Shaker. Your trust and support are greatly appreciated.
Our Incubator Shaker Series is designed for shaking and heating samples with optional
CO2 and humidity control to meet the research and experiment needs in modern
biotechnology. Our state-of-the-art embedded microprocessor system utilized in this
machine has powerful data processing ability, outstanding stability, excellent
interference resistance, and high precision control of the temperature and shaking
performance of the instrument. Sophisticated manufacturing processes, aesthetic product
design, and a user-friendly interface are elegantly incorporated into the production of the
instrument.
The Incubator Shaker is widely used in biology, microbiology, medicine, pharmaceutics,
food science, and environmental science, which include various biological and chemical
reactions that require high precision control of temperature and shaking. Applicable
processes include bacteria culture, fermentation, and hybridization, and enzyme and cell
tissue research. It is also applicable to both static and dynamic culture of microbial cells
and strains.
Table of Contents
1. Overview
2. Unpacking Guide
3. Preparation
4. Safety Instructions
5. Installation
6. Structure
7. Control Panel
8. Settings
9. Operating Instructions
10. Maintenance
11. Troubleshooting
12. Method of Use Accessories
13. Optional Accessories
14. Warranty
P9
P13
P14
P18
P19
P20
P31
P32
P33
P35
P43
P45
P7

1.2 Features
12
1.1 Description
1. Overview 1. Overview
The AEOLUS™ CO2 incubator shaker with controlled temperature, shaking, humidity,
and CO2 concentration can be stacked three units high, while occupying the floor space
of only one unit. The combination and innovation of Proportional-Integral-Derivative (PID)
and Fuzzy control technology have allowed for high precision control of the instrument’s
chamber temperature. The three axis eccentric balance mechanism is stable, reliable,
and durable. The speed control system has a speed terminal feedback system, which
ensures high precision oscillation within the instrument. An inverter compressor is used
for refrigeration, thus making cooling a highly efficient process with low energy
consumption. The instrument’s noise reduction design keeps the noise level to only
55dB. The independent modular operation design makes it easy to selectively choose
which functions to run, so it can be used as a CO2 incubator for culturing cells statically,
or as an ordinary open air shaker, or as a biochemical incubator.
This machine can run continuously, but also includes a timer which can be set up to 999
hours. 12 segment functions can be programmed to achieve diverse environment
conditions, and specific running times for each segment can be set to create the required
environments. The rail platform makes it convenient to place and retrieve samples in the
instrument. The 7 inch color touch screen provides easy and streamlined operation.
Additionally, the WiFi monitoring system can be equipped with multiple phones, allowing
multiple users to remotely monitor the same instrument. Remote monitoring also sends
timely alarm information to deal with emergency situations quickly and safely without air
intake to protect cultured cells.
ŸGood thermal insulation, air tightness and waterproof properties: The main box
body, door, and insulation materials of the instrument all use foamed polyurethane
materials, which have the advantages of lightness, sound insulation, shockproof,
electrical insulation, heat resistance, cold resistance, solvent resistance, and aging
resistance.
ŸAutomatic adjustment of PID control: Instrument adjusts itself to the appropriate
parameters according to the different user set conditions and ensures high
precision and stability of temperature.
ŸStrong cooling capacity: Chamber can be cooled to 20°C below the ambient
temperature (Lowest temp is 4℃).
ŸExcellent temperature control performance: When stable at 37°C, the uniformity in
the cavity reaches ≤ ±0.2°C.
ŸPull out rail platform: Operator can easily pull the platform out to load or unload
fixtures and then push it back and lock. Only one locking handle in the middle of the
platform provides fast and convenient operation.
ŸIntuitive visual 7 inch color touch screen: Modular display shows the different
functions.
ŸCapacitive touch screen: The capacitive screen is durable and has no reaction
delay when the screen is pressed.
ŸSimple screen interface: A variety of functions, set values, and actual values are
displayed. At a glance, the user will know all the running parameters as they are
easy to find and operate.
ŸThe display screen has an automatic lock screen, automatic sleep function, and
password protection functions: Prevent mis-operation or allowing non-authorized
personnel to perform non-permissible operations.
ŸThree units can be stacked: Instrument can be purchased as a single unit, double
stack, or triple stack with the option to stack units (up to triple stack) in the future.
ŸBi-directional ultraviolet sterilization: The chamber is equipped with two sets of UV
sterilization light components to enhance the germicidal effect and reach every part
of the chamber.
ŸInverter compressor: Noiseless, durable, highly efficient, and energy saving.
ŸUltra quiet design: The whole instrument runs very quietly; the noise level is less
than 55dB, giving the operator a quiet environment.
ŸSlow acceleration and deceleration: Shaking function starts and stops slowly to
prevent large shear forces on the bacteria or cells.
ŸHigh pressure water rinse for the platform and bottom of the chamber: Simple
cleaning that is fast and efficient.

1.2 Features
12
1.1 Description
1. Overview 1. Overview
The AEOLUS™ CO2 incubator shaker with controlled temperature, shaking, humidity,
and CO2 concentration can be stacked three units high, while occupying the floor space
of only one unit. The combination and innovation of Proportional-Integral-Derivative (PID)
and Fuzzy control technology have allowed for high precision control of the instrument’s
chamber temperature. The three axis eccentric balance mechanism is stable, reliable,
and durable. The speed control system has a speed terminal feedback system, which
ensures high precision oscillation within the instrument. An inverter compressor is used
for refrigeration, thus making cooling a highly efficient process with low energy
consumption. The instrument’s noise reduction design keeps the noise level to only
55dB. The independent modular operation design makes it easy to selectively choose
which functions to run, so it can be used as a CO2 incubator for culturing cells statically,
or as an ordinary open air shaker, or as a biochemical incubator.
This machine can run continuously, but also includes a timer which can be set up to 999
hours. 12 segment functions can be programmed to achieve diverse environment
conditions, and specific running times for each segment can be set to create the required
environments. The rail platform makes it convenient to place and retrieve samples in the
instrument. The 7 inch color touch screen provides easy and streamlined operation.
Additionally, the WiFi monitoring system can be equipped with multiple phones, allowing
multiple users to remotely monitor the same instrument. Remote monitoring also sends
timely alarm information to deal with emergency situations quickly and safely without air
intake to protect cultured cells.
ŸGood thermal insulation, air tightness and waterproof properties: The main box
body, door, and insulation materials of the instrument all use foamed polyurethane
materials, which have the advantages of lightness, sound insulation, shockproof,
electrical insulation, heat resistance, cold resistance, solvent resistance, and aging
resistance.
ŸAutomatic adjustment of PID control: Instrument adjusts itself to the appropriate
parameters according to the different user set conditions and ensures high
precision and stability of temperature.
ŸStrong cooling capacity: Chamber can be cooled to 20°C below the ambient
temperature (Lowest temp is 4℃).
ŸExcellent temperature control performance: When stable at 37°C, the uniformity in
the cavity reaches ≤ ±0.2°C.
ŸPull out rail platform: Operator can easily pull the platform out to load or unload
fixtures and then push it back and lock. Only one locking handle in the middle of the
platform provides fast and convenient operation.
ŸIntuitive visual 7 inch color touch screen: Modular display shows the different
functions.
ŸCapacitive touch screen: The capacitive screen is durable and has no reaction
delay when the screen is pressed.
ŸSimple screen interface: A variety of functions, set values, and actual values are
displayed. At a glance, the user will know all the running parameters as they are
easy to find and operate.
ŸThe display screen has an automatic lock screen, automatic sleep function, and
password protection functions: Prevent mis-operation or allowing non-authorized
personnel to perform non-permissible operations.
ŸThree units can be stacked: Instrument can be purchased as a single unit, double
stack, or triple stack with the option to stack units (up to triple stack) in the future.
ŸBi-directional ultraviolet sterilization: The chamber is equipped with two sets of UV
sterilization light components to enhance the germicidal effect and reach every part
of the chamber.
ŸInverter compressor: Noiseless, durable, highly efficient, and energy saving.
ŸUltra quiet design: The whole instrument runs very quietly; the noise level is less
than 55dB, giving the operator a quiet environment.
ŸSlow acceleration and deceleration: Shaking function starts and stops slowly to
prevent large shear forces on the bacteria or cells.
ŸHigh pressure water rinse for the platform and bottom of the chamber: Simple
cleaning that is fast and efficient.

ŸAutomatic defrosting function: The machine automatically defrosts according to its
own needs, no need to enter the prescribed time or any other settings.
ŸEnergy saving control: The advanced Polyurethene material foaming process
improves the insulation performance, reduces energy consumption, and saves more
electrical energy.
ŸDC brushless motor drive: Low noise, high efficiency, no maintenance, long life,
minimal heat, no effect on the chamber temperature.
ŸDouble cross flow fan driving air circulation: Wind pressure is high, large air volume,
low noise, low heating capacity and long life.
Ÿ12 automatic programmable sections control function: User can set 12 different
speeds, temperatures, and times to automatically run the instrument at.
ŸAcousto-optic alarm and protection functions: Over temperature alarm, over speed
alarm, sensor fault alarm, timing alarm, open door alarm, over CO2 concentration
alarm, over humidity alarm, electric leakage protection, no intake alarm (Instrument
with CO2 function)
ŸOpen door protection: When the door opens, the machine stops running to protect the
safety of the operator.
ŸAutomatic recovery run: When the operation stops as a result of power failure, after
the power is supplied again, the instrument automatically resumes operation
according to the original parameters of operation.
ŸLong timing: User can set 0-999 hours and 59 minutes of operation time; the touch
screen displays the remaining time, and when time runs up the instrument prompts
"Program Finish".
ŸPowerful data storage function: Data can be stored for more than 2 years. Data is
recorded in 1 minute intervals.
ŸReal-time data query function: On the display, operator can query the running data
from the latest 7 days. Data is recorded in 1 minute intervals.
ŸU disk export data function: With a USB port, it is easy to export more than 2 years of
running data records to the USB.
ŸAlarm information recording and query function: Alarm information memory is stored
and can be queried.
ŸDisplay has a real-time temperature curve display function: Historical data and real-
time data is displayed on the same diagram display and is easy to check.
ŸDisplay can be switched from English interface to Chinese interface: If user is used to
the Chinese interface, it is easy to switch at any time.
ŸRemote WiFi monitor accessory: Instrument can be remotely controlled through
wireless LAN monitoring or internet mobile phone. Many mobile phones can monitor
the same instrument.
ŸComputer monitoring software accessory: Instrument can be monitored by a LAN
wired computer monitoring, and can remotely send alarm messages to connected
mobile phones.
1. Overview 1. Overview
34
And more on the instrument version with CO2 functions:
ŸCO2 infrared sensor: Accurate control, sensitive response, stable performance and
long life. It is not affected by any changes in temperature or humidity. Match the full
set of CO2 control accessories.
ŸMulti High-Efficiancy-Particulate-Air (HEPA) filters: Prevent gas impurities in the
cylinder or pollution source from contaminating the gas in the chamber.
ŸEasy cleaning inner chamber: It is easy to wipe and disinfect the inner chamber
without dismantling the platform to minimize the growth of bacteria.
ŸDouble hollow glass window: Heating and antifogging design allow the cell culture in
the chamber to be clear at a glance.
ŸHigh humidity, anti-rust, and anti-condensation design: Prevents the door from rusting
due to high humidity. Prevents rust in the internal chamber. Prevents the micro flow of
water on the door to the ground when the door is open.
ŸAutomatically liquid filled device accessory: The liquid can be automatically filled to
the wet disk when the passive humidifying liquid is evaporated and dried in the
chamber.
ŸActive humidification device accessory: The humidity in the chamber can be
controlled.
Procedure for testing the CO2 control function unit with RH Controller:
In order to ensure the stability of the humidity in the chamber, it is recommended that
flasks be arranged evenly and that circulation and ventilation is ensured in the chamber.
The specific layout method and the number of flasks are as follows:
15
12
80%~85%
70%
Set
RH Value
Max. QTY
of the Flask Place Test conditions
Take 250ml flask as an example:
1. Fill 250ml flask with 100ml pure water
2. Set the temperature of the instrument:
37℃
3. Set the speed of the instrument: 25rpm1
4. Ambient temperature: 20℃~25℃

ŸAutomatic defrosting function: The machine automatically defrosts according to its
own needs, no need to enter the prescribed time or any other settings.
ŸEnergy saving control: The advanced Polyurethene material foaming process
improves the insulation performance, reduces energy consumption, and saves more
electrical energy.
ŸDC brushless motor drive: Low noise, high efficiency, no maintenance, long life,
minimal heat, no effect on the chamber temperature.
ŸDouble cross flow fan driving air circulation: Wind pressure is high, large air volume,
low noise, low heating capacity and long life.
Ÿ12 automatic programmable sections control function: User can set 12 different
speeds, temperatures, and times to automatically run the instrument at.
ŸAcousto-optic alarm and protection functions: Over temperature alarm, over speed
alarm, sensor fault alarm, timing alarm, open door alarm, over CO2 concentration
alarm, over humidity alarm, electric leakage protection, no intake alarm (Instrument
with CO2 function)
ŸOpen door protection: When the door opens, the machine stops running to protect the
safety of the operator.
ŸAutomatic recovery run: When the operation stops as a result of power failure, after
the power is supplied again, the instrument automatically resumes operation
according to the original parameters of operation.
ŸLong timing: User can set 0-999 hours and 59 minutes of operation time; the touch
screen displays the remaining time, and when time runs up the instrument prompts
"Program Finish".
ŸPowerful data storage function: Data can be stored for more than 2 years. Data is
recorded in 1 minute intervals.
ŸReal-time data query function: On the display, operator can query the running data
from the latest 7 days. Data is recorded in 1 minute intervals.
ŸU disk export data function: With a USB port, it is easy to export more than 2 years of
running data records to the USB.
ŸAlarm information recording and query function: Alarm information memory is stored
and can be queried.
ŸDisplay has a real-time temperature curve display function: Historical data and real-
time data is displayed on the same diagram display and is easy to check.
ŸDisplay can be switched from English interface to Chinese interface: If user is used to
the Chinese interface, it is easy to switch at any time.
ŸRemote WiFi monitor accessory: Instrument can be remotely controlled through
wireless LAN monitoring or internet mobile phone. Many mobile phones can monitor
the same instrument.
ŸComputer monitoring software accessory: Instrument can be monitored by a LAN
wired computer monitoring, and can remotely send alarm messages to connected
mobile phones.
1. Overview 1. Overview
34
And more on the instrument version with CO2 functions:
ŸCO2 infrared sensor: Accurate control, sensitive response, stable performance and
long life. It is not affected by any changes in temperature or humidity. Match the full
set of CO2 control accessories.
ŸMulti High-Efficiancy-Particulate-Air (HEPA) filters: Prevent gas impurities in the
cylinder or pollution source from contaminating the gas in the chamber.
ŸEasy cleaning inner chamber: It is easy to wipe and disinfect the inner chamber
without dismantling the platform to minimize the growth of bacteria.
ŸDouble hollow glass window: Heating and antifogging design allow the cell culture in
the chamber to be clear at a glance.
ŸHigh humidity, anti-rust, and anti-condensation design: Prevents the door from rusting
due to high humidity. Prevents rust in the internal chamber. Prevents the micro flow of
water on the door to the ground when the door is open.
ŸAutomatically liquid filled device accessory: The liquid can be automatically filled to
the wet disk when the passive humidifying liquid is evaporated and dried in the
chamber.
ŸActive humidification device accessory: The humidity in the chamber can be
controlled.
Procedure for testing the CO2 control function unit with RH Controller:
In order to ensure the stability of the humidity in the chamber, it is recommended that
flasks be arranged evenly and that circulation and ventilation is ensured in the chamber.
The specific layout method and the number of flasks are as follows:
15
12
80%~85%
70%
Set
RH Value
Max. QTY
of the Flask Place Test conditions
Take 250ml flask as an example:
1. Fill 250ml flask with 100ml pure water
2. Set the temperature of the instrument:
37℃
3. Set the speed of the instrument: 25rpm1
4. Ambient temperature: 20℃~25℃

Model
1.3 Specifications
0 (Stop),30~300 RPM
1. Overview 1. Overview
6
Room Temp. Minus 20℃~60℃ (Lowest Temp. is 4℃)
5
Speed
Speed Accuracy
Max. Load
Platform Dimensions
0~999Hours 59min.
Yes
Yes
Yes
Yes
1 min.
Timer
Automatic Defrost
Heat Power
Cool Power
Max Power
U Disk Download Date
USB Data Export
Date Storage Interval
Most Recent 912 Days of Data
Noise Level
Water Sparge Resistant
Interface Languages English or Chinese
Auto-run after
Power Recovery
≤1000W
IS-18CA IS-18CA
±1rpm
Model
Temp. Range
≤55db (When Compressor is Running)
≤ ±0.2°C (@37°C)
≤300W
≤800W
20kg (44.1lb)
900×550mm (35.4 x 21.6in)
Orbit Diameter
CO2 Sensor
CO2 Control Range 0~20%
Ø26mm (Ø1.02in)
Data Stored
Time, Temp, Speed,
CO2 Concentration,
RH, Instrument Status
Power Supply
Net Weight
AC110V+11V, 60Hz
195kg (430lb)
Mobile Monitoring and
WiFi Control
PC Monitor and Control
by RS485 (Option)
Optional
Optional
Optional
Optional
CO2 Display Accuracy
Temperature Accuracy
Down-Swing (Optional up-swing door for top unit of triple stack)
95%
CO2 Control Accuracy
CO2 Recovery Time
after Door Open for 30s
Max. Humidity
Door Open Direction
Programmable
Segments 12 Segments
Relative Humidity Type Active Humidification
Disinfection UV Lamps on Left and Right Walls
Parameter Protection
Screen Data Display
Password Protected
Displays Most Recent 7 Days of Data
Data Curve Display Temp. Curve, Speed Curve, CO2 Curve, RH Curve
Max. Capacity
±0.1%( @5% )
≤6min (@37℃)
≤6min(@5%)
Clamp Capacity: 25mlx134/ 50ml×134/ 100ml×60/ 150ml×60/ 200ml×60/
250ml×60/ 500ml×32/ 1000ml×22/ 2000ml×15/ 3000ml×12/ 5000ml×6
Sticky Mat: 25mlx187/ 50ml×187/ 100ml×112/ 150ml×104/ 200ml×84/
250ml×76/ 500ml×40/ 1000ml×28/ 2000ml×15/ 3000ml×12/ 5000ml×6
5~60℃
Temp Recovery after
Door Open for 30s
Display 7” Color Capacitance Touch Screen
Touch Screen Type Capacitance
Screen Auto-Lock Screen Requires Password After Set Screen Inactivity Time
Screen Auto-Sleep Screen Sleeps After Set Screen Inactivity Time
±0.1%
IR
Temperature Uniformity
Temperature Stability
RH Control
Passive Humidifier
Automatic Water
Application range of
CO2 temperature
1380×875×530(+80 w/ feet)mm (54.33×34.45×20.87(+3.15 w/ feet)in)
Internal Dimensions
External Dimensions
985×775×410mm (38.78×30.51×16.14in)
≤ ±0.2°C (@37°C)
≤ ±0.1°C (@37°C)
IS-18A
NA
NA
NA
NA
NA
NA
NA
NA
IS-18A
Time, Temp, Speed,
Instrument Status
Illumination LED Lamps on Left and Right Walls

Model
1.3 Specifications
0 (Stop),30~300 RPM
1. Overview 1. Overview
6
Room Temp. Minus 20℃~60℃ (Lowest Temp. is 4℃)
5
Speed
Speed Accuracy
Max. Load
Platform Dimensions
0~999Hours 59min.
Yes
Yes
Yes
Yes
1 min.
Timer
Automatic Defrost
Heat Power
Cool Power
Max Power
U Disk Download Date
USB Data Export
Date Storage Interval
Most Recent 912 Days of Data
Noise Level
Water Sparge Resistant
Interface Languages English or Chinese
Auto-run after
Power Recovery
≤1000W
IS-18CA IS-18CA
±1rpm
Model
Temp. Range
≤55db (When Compressor is Running)
≤ ±0.2°C (@37°C)
≤300W
≤800W
20kg (44.1lb)
900×550mm (35.4 x 21.6in)
Orbit Diameter
CO2 Sensor
CO2 Control Range 0~20%
Ø26mm (Ø1.02in)
Data Stored
Time, Temp, Speed,
CO2 Concentration,
RH, Instrument Status
Power Supply
Net Weight
AC110V+11V, 60Hz
195kg (430lb)
Mobile Monitoring and
WiFi Control
PC Monitor and Control
by RS485 (Option)
Optional
Optional
Optional
Optional
CO2 Display Accuracy
Temperature Accuracy
Down-Swing (Optional up-swing door for top unit of triple stack)
95%
CO2 Control Accuracy
CO2 Recovery Time
after Door Open for 30s
Max. Humidity
Door Open Direction
Programmable
Segments 12 Segments
Relative Humidity Type Active Humidification
Disinfection UV Lamps on Left and Right Walls
Parameter Protection
Screen Data Display
Password Protected
Displays Most Recent 7 Days of Data
Data Curve Display Temp. Curve, Speed Curve, CO2 Curve, RH Curve
Max. Capacity
±0.1%( @5% )
≤6min (@37℃)
≤6min(@5%)
Clamp Capacity: 25mlx134/ 50ml×134/ 100ml×60/ 150ml×60/ 200ml×60/
250ml×60/ 500ml×32/ 1000ml×22/ 2000ml×15/ 3000ml×12/ 5000ml×6
Sticky Mat: 25mlx187/ 50ml×187/ 100ml×112/ 150ml×104/ 200ml×84/
250ml×76/ 500ml×40/ 1000ml×28/ 2000ml×15/ 3000ml×12/ 5000ml×6
5~60℃
Temp Recovery after
Door Open for 30s
Display 7” Color Capacitance Touch Screen
Touch Screen Type Capacitance
Screen Auto-Lock Screen Requires Password After Set Screen Inactivity Time
Screen Auto-Sleep Screen Sleeps After Set Screen Inactivity Time
±0.1%
IR
Temperature Uniformity
Temperature Stability
RH Control
Passive Humidifier
Automatic Water
Application range of
CO2 temperature
1380×875×530(+80 w/ feet)mm (54.33×34.45×20.87(+3.15 w/ feet)in)
Internal Dimensions
External Dimensions
985×775×410mm (38.78×30.51×16.14in)
≤ ±0.2°C (@37°C)
≤ ±0.1°C (@37°C)
IS-18A
NA
NA
NA
NA
NA
NA
NA
NA
IS-18A
Time, Temp, Speed,
Instrument Status
Illumination LED Lamps on Left and Right Walls

78
Unpacking Procedure
Warning
ŸDue to its weight, please use a lift or a forklift or other lifting apparatus to transport or
move the instrument. DO NOT TRANSPORT OR MOVE THE INSTRUMENT WITHOUT
HELPERS.
ŸInspect contents upon receiving the instrument, if the instrument is upside down,
contact the manufacturer immediately.
ŸMake sure the power is disconnected and the instrument is not loaded when moving
the instrument. Please move instrument individually before stacking them.
2. Unpacking Guide 2. Unpacking Guide
Equipped in type of CO2 Model only
Equipped in type of CO2 Model only
Equipped in type of CO2 Model only
Equipped in type of CO2 Model only
Power Cord
Liquid Collection Tray
Fuse
Cross Screwdriver 2#
Single open-end Wrench 24
Wrench S10
User Manual
Filter
Filter Protection Package
Hood
PU Pipe (5 m)
Silicone Tube 12×2Ø
T-Connectors 14Ø
Two-way Connectors 14Ø
Three-way Connectors
1
1
1
1
1
1
1
1
1
1
4
1
1m
1
1
2
Packing List
Accessories
Platform handle backup
Name Qty Remark
Stackable Incubator Shaker
AC250V/10A
Equipped in type of CO2 Model only
1. Lift the instrument with a lift or forklift and place the instrument on a level surface with
caution.
2. Remove the protective packing material and inspect if the instrument is damaged.
3. Please find the packing list, in the user manual, in the plastic documents pouch inside
the shipment box.
3. Check the packing list in the manual to make sure there are no missing accessories;
if there are please contact Crystal Technology immediately.

78
Unpacking Procedure
Warning
ŸDue to its weight, please use a lift or a forklift or other lifting apparatus to transport or
move the instrument. DO NOT TRANSPORT OR MOVE THE INSTRUMENT WITHOUT
HELPERS.
ŸInspect contents upon receiving the instrument, if the instrument is upside down,
contact the manufacturer immediately.
ŸMake sure the power is disconnected and the instrument is not loaded when moving
the instrument. Please move instrument individually before stacking them.
2. Unpacking Guide 2. Unpacking Guide
Equipped in type of CO2 Model only
Equipped in type of CO2 Model only
Equipped in type of CO2 Model only
Equipped in type of CO2 Model only
Power Cord
Liquid Collection Tray
Fuse
Cross Screwdriver 2#
Single open-end Wrench 24
Wrench S10
User Manual
Filter
Filter Protection Package
Hood
PU Pipe (5 m)
Silicone Tube 12×2Ø
T-Connectors 14Ø
Two-way Connectors 14Ø
Three-way Connectors
1
1
1
1
1
1
1
1
1
1
4
1
1m
1
1
2
Packing List
Accessories
Platform handle backup
Name Qty Remark
Stackable Incubator Shaker
AC250V/10A
Equipped in type of CO2 Model only
1. Lift the instrument with a lift or forklift and place the instrument on a level surface with
caution.
2. Remove the protective packing material and inspect if the instrument is damaged.
3. Please find the packing list, in the user manual, in the plastic documents pouch inside
the shipment box.
3. Check the packing list in the manual to make sure there are no missing accessories;
if there are please contact Crystal Technology immediately.

9
ŸPay attention to avoid any risk of body injuries from disassembling, lifting, or
moving the instrument.
ŸThe CO2 concentration may increase and can be harmful to your health if
operating the instrument in a small and airtight room.
ŸPlease keep the room ventilated when the CO2 function is on. Additionally, please
avoid direct inhalation of the gas when opening the door.
ŸKeep the vent of the instrument away from heat sources or other vents, otherwise
the compressor cannot be started or started frequently during refrigeration.
ŸWhen the instruments are stacked, make sure the floor loading capacity is at
least 1.5 times of the instruments weight. Stack them up near the wall or on the
floor with a reinforced beam to ensure good loading capacity.
The instrument must be placed on a firm and level surface. The total weight of the
instrument when fully loaded (474 lb/unit) must be taken into consideration. There
must be sufficient space left around the instrument. There should be at least 20 cm
between the instrument and any wall or any two adjacent instruments.
3.1 Placement Conditions
Warning
3.2 Ambient Conditions
a. F or indoor use only
b. A mbient Temperature: 10℃-35℃
c. R elative Humidity: 20%-80% RH
d.Atmospheric Pressure: 75kPa-106kPa
e. Use a dedicated power outlet with a capacity no less than 1.3 kW and AC 110 V
f. The fluctuations of power supply voltage is less than 10% of rated voltage
3. Preparation3. Preparation
3.3 Space Conditions
10
●3 Down-swing-door instruments are stacked as below:
1290mm
270mm
800mm
880mm
80 530mm 530mm 530mm
1670mm
mm
1330mm
Note: When double and triple stacked, it is recommended that the second and third
instruments are not used at speeds exceeding 250 RPM.

9
ŸPay attention to avoid any risk of body injuries from disassembling, lifting, or
moving the instrument.
ŸThe CO2 concentration may increase and can be harmful to your health if
operating the instrument in a small and airtight room.
ŸPlease keep the room ventilated when the CO2 function is on. Additionally, please
avoid direct inhalation of the gas when opening the door.
ŸKeep the vent of the instrument away from heat sources or other vents, otherwise
the compressor cannot be started or started frequently during refrigeration.
ŸWhen the instruments are stacked, make sure the floor loading capacity is at
least 1.5 times of the instruments weight. Stack them up near the wall or on the
floor with a reinforced beam to ensure good loading capacity.
The instrument must be placed on a firm and level surface. The total weight of the
instrument when fully loaded (474 lb/unit) must be taken into consideration. There
must be sufficient space left around the instrument. There should be at least 20 cm
between the instrument and any wall or any two adjacent instruments.
3.1 Placement Conditions
Warning
3.2 Ambient Conditions
a. F or indoor use only
b. A mbient Temperature: 10℃-35℃
c. R elative Humidity: 20%-80% RH
d.Atmospheric Pressure: 75kPa-106kPa
e. Use a dedicated power outlet with a capacity no less than 1.3 kW and AC 110 V
f. The fluctuations of power supply voltage is less than 10% of rated voltage
3. Preparation3. Preparation
3.3 Space Conditions
10
●3 Down-swing-door instruments are stacked as below:
1290mm
270mm
800mm
880mm
80 530mm 530mm 530mm
1670mm
mm
1330mm
Note: When double and triple stacked, it is recommended that the second and third
instruments are not used at speeds exceeding 250 RPM.

●Stacking Rack (IS-A118) and 2 Down-swing-door instruments are stacked as
below:
●2 Down-swing-door instruments and 1 Up-swing-door instrument are stacked as
below:
12
11
3. Preparation3. Preparation
1060mm
530mm
1290mm
880mm
340mm 530mm 530mm
1400mm
1290mm
530mm 530mm 530mm
1332mm
800mm
270mm
2055mm
80
mm
Note: When double and triple stacked, it is recommended that the second and third
instruments are not used at speeds exceeding 250 RPM.
Note: When double and triple stacked, it is recommended that the second and third
instruments are not used at speeds exceeding 250 RPM.

●Stacking Rack (IS-A118) and 2 Down-swing-door instruments are stacked as
below:
●2 Down-swing-door instruments and 1 Up-swing-door instrument are stacked as
below:
12
11
3. Preparation3. Preparation
1060mm
530mm
1290mm
880mm
340mm 530mm 530mm
1400mm
1290mm
530mm 530mm 530mm
1332mm
800mm
270mm
2055mm
80
mm
Note: When double and triple stacked, it is recommended that the second and third
instruments are not used at speeds exceeding 250 RPM.
Note: When double and triple stacked, it is recommended that the second and third
instruments are not used at speeds exceeding 250 RPM.

: This instrument contains flammable refrigerant. Be careful when installing and
operating to prevent the risks of combustion or explosion.
: Risk of burns may be created when operating or maintaining.
: Risk of hand injuries due to improper operation or accidents when opening the
door.
Please make sure to follow the safety instructions!
● Please use a lift or a forklift or other lifting apparatus to raise or move the instrument.
● Make sure the power supply voltage matches the voltage of the instrument before it is
connected.
● The instrument must be properly placed.
● Use a grounded power socket.
● Do not drag the power cord when unplugging.
● Do not use a damaged or non-designated power cord.
● Only qualified personnel are allowed to open the control box.
● Keep the handle on the platform screwed when the instrument is running.
● Keep the room ventilated.
Disconnect the power supply in any of the situations below:
● Moving or transporting the instrument
● Opening the power box
● Changing the fuse
● Malfunctioning of the instrument
● Instrument is out of use for a long period of time
4. Safety Instructions 5. Installation
Lifting the instrument by hand should be prohibited. Please use a lift or a forklift or other
lifting apparatus to raise or move the instrument.
Note
5.1 Tool Requirements
a. H ex wrench S10. Cross screwdriver #2. Single open-end wrench 24
b. A lift or forklift or other lifting apparatus with a minimum loading capacity
of 350 kg (772 lb).
5.2 Installation
a. Ensure the installation site meets the requirement as instructed in Section 3.1.
b. First unit installation: Raise adjustable feet away from the ground.
c. Slowly and smoothly move the instrument on its wheels to its designated location.
d. Loosen the four adjustable feet with a wrench to touch the ground, keep it level, then
tighten the four adjustable feet with a wrench. The instrument needs to be stable and
cannot be moved.
14
13
Adjust the height of the feet to allow the instrument to move or be set:
Adjust all feet up to off the
ground so the instrument can
move on its wheels.
Adjust the feet down to the
ground, the instrument will be
fixed.

: This instrument contains flammable refrigerant. Be careful when installing and
operating to prevent the risks of combustion or explosion.
: Risk of burns may be created when operating or maintaining.
: Risk of hand injuries due to improper operation or accidents when opening the
door.
Please make sure to follow the safety instructions!
● Please use a lift or a forklift or other lifting apparatus to raise or move the instrument.
● Make sure the power supply voltage matches the voltage of the instrument before it is
connected.
● The instrument must be properly placed.
● Use a grounded power socket.
● Do not drag the power cord when unplugging.
● Do not use a damaged or non-designated power cord.
● Only qualified personnel are allowed to open the control box.
● Keep the handle on the platform screwed when the instrument is running.
● Keep the room ventilated.
Disconnect the power supply in any of the situations below:
● Moving or transporting the instrument
● Opening the power box
● Changing the fuse
● Malfunctioning of the instrument
● Instrument is out of use for a long period of time
4. Safety Instructions 5. Installation
Lifting the instrument by hand should be prohibited. Please use a lift or a forklift or other
lifting apparatus to raise or move the instrument.
Note
5.1 Tool Requirements
a. H ex wrench S10. Cross screwdriver #2. Single open-end wrench 24
b. A lift or forklift or other lifting apparatus with a minimum loading capacity
of 350 kg (772 lb).
5.2 Installation
a. Ensure the installation site meets the requirement as instructed in Section 3.1.
b. First unit installation: Raise adjustable feet away from the ground.
c. Slowly and smoothly move the instrument on its wheels to its designated location.
d. Loosen the four adjustable feet with a wrench to touch the ground, keep it level, then
tighten the four adjustable feet with a wrench. The instrument needs to be stable and
cannot be moved.
14
13
Adjust the height of the feet to allow the instrument to move or be set:
Adjust all feet up to off the
ground so the instrument can
move on its wheels.
Adjust the feet down to the
ground, the instrument will be
fixed.

e. To stack the 2nd or 3rd unit, fix it with four screws at the designated locations below
on both sides.
16
5. Installation 5. Installation
Please make sure each instrument is placed level, stable and still. Do not exceed the
maximum load of 20kg and the maximum stacked shaker speed of 250 RPM on the
second and third units.
Note
a. When stacked up to 3 units, units will need a Two-way Connector and 2 T-connectors.
b. When stacked up to 2 units, units will need a Two-way Connector and a T-connector.
5.3 Drain connection
2. Using the polyurethane tube provided,
connect the regulator to a CO2 inlet HEPA
filter first, and then to the CO2 inlet located
on the right side of the shaker. (See Figure 1)
3. Set the CO2 secondary flow control valve to 0.08~ 0.1MPa
(11.6~14.5psi).
(WARNING: Excessive pressure may cause disconnection
of internal pipes inside the CO2 incubator which will result
in leakage of CO2 gas into the surrounding environment.
High CO2 concentration can cause harm to your
health and may cause asphyxia or death. Maintenance
is required if the inline tube falls off.)
5.4 CO2 Connection
1. Install a regulator with flow control valve onto the CO2 Cylinder. Please use a
2 2
regulator with a primary preset pressure of 25MPa (250kg/cm , 3500l b/in ), and a
secondary preset pressure of 0.6MPa (2.0kg/cm, 30psiG).
Gas Inlet
PU Pipe
Filter
Protection Cover
Figure 1
Figure 2
Two-way
Connector
T-connector
15

e. To stack the 2nd or 3rd unit, fix it with four screws at the designated locations below
on both sides.
16
5. Installation 5. Installation
Please make sure each instrument is placed level, stable and still. Do not exceed the
maximum load of 20kg and the maximum stacked shaker speed of 250 RPM on the
second and third units.
Note
a. When stacked up to 3 units, units will need a Two-way Connector and 2 T-connectors.
b. When stacked up to 2 units, units will need a Two-way Connector and a T-connector.
5.3 Drain connection
2. Using the polyurethane tube provided,
connect the regulator to a CO2 inlet HEPA
filter first, and then to the CO2 inlet located
on the right side of the shaker. (See Figure 1)
3. Set the CO2 secondary flow control valve to 0.08~ 0.1MPa
(11.6~14.5psi).
(WARNING: Excessive pressure may cause disconnection
of internal pipes inside the CO2 incubator which will result
in leakage of CO2 gas into the surrounding environment.
High CO2 concentration can cause harm to your
health and may cause asphyxia or death. Maintenance
is required if the inline tube falls off.)
5.4 CO2 Connection
1. Install a regulator with flow control valve onto the CO2 Cylinder. Please use a
2 2
regulator with a primary preset pressure of 25MPa (250kg/cm , 3500l b/in ), and a
secondary preset pressure of 0.6MPa (2.0kg/cm, 30psiG).
Gas Inlet
PU Pipe
Filter
Protection Cover
Figure 1
Figure 2
Two-way
Connector
T-connector
15

1. Do not move the pressure adjustment rod on the regulator when changing the CO2
cylinder. It will affect the secondary pressure and will result in needing to reset the
valve. The inline tube might fall off if the pressure is too high and maintenance will be
required.
2. Check tube regularly to ensure it is safely connected. Change the tube if it is
damaged or aged.
3. Use the stainless steel hoop to tighten the connection point of the Polyurethane tube
and the filter to prevent the pipe from falling off and leaking gas.
4. Use more than 99.99% pure CO2. Low purity may cause blocking of the filter or
damage of the electromagnetic valve.
5.Connection components
A. Schematic diagram B. Actual picture
4. Check the tube connections for possible leakage (the connections between tube and
regulator, tube and access port, and tube and filter).
Note:
5. Installation
17 18
Polyurethane Pipe
(Inner dia.7mm,
outer dia. 10mm)
To the filter
Stainless steel hoop
To the CO2 regulator
6. Structure
●Down-swing-door
●Up-swing-door
Ventilation
Ventilation
Adjustable feet
Adjustable feet
Handle
Handle
Touchscreen Control Interface
WIFI
Interface
USB
Data Out
UV lamp switch
Power switch
Remote wifi monitor interface
(optional)
Co2 Gas Inlet
I
O
I
O
USB port
Warnings
- Make sure the gas supply meets the specified requirements.
- Make sure all the pipes are properly connected.
- Make sure the gas pressure settings are correct.
- Wrong pipe connection or pressure settings may cause CO2 leakage.
- High Co2 concentration will do harm to your health and may cause
suffocation or death.
- Alternative methods should be taken if there is no sufficient ventilation in the
room to guarantee safety. These methods include gas monitoring and alarming
system.
- Maintaining correct gas pressures can prevent gas leakage.
Touchscreen Control Interface
This manual suits for next models
1
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