DMT 720MO User manual

1TISSUE BATH MYOGRAPH SYSTEM - 720MO - USER GUIDE
USER GUIDE

TISSUE BATH  720MO
USER GUIDE

3TISSUE BATH MYOGRAPH SYSTEM - 720MO - USER GUIDE
CONTENTS
Chapter 1 - tissue bath overview..............................................................................................................................................................................................3
Chapter 2 - setting up the tissue bath ..................................................................................................................................................................................4
2.1 Changing and adjusting the mounting supports .............................................................................................................................4
2.1.1 Changing the mounting supports (gure 2.1): ............................................................................................................................4
2.1.2 Fine adjusting the pins for larger vessels (gure 2.2 and 2.3) ...................................................................................................5
2.2 Calibration of the force transducer ..................................................................................................................................................5
Chapter 3 - Experimental set-up ..............................................................................................................................................................................................6
3.1 Mounting protocol ?? ........................................................................................................................................................................6
3.2 Normalization ....................................................................................................................................................................................7
3.2.1 Principles of the normalization procedure ...................................................................................................................................7
3.3 Standard start....................................................................................................................................................................................8
3.3.1 Principles of the standard start procedure ..................................................................................................................................8
3.4 Endothelium function........................................................................................................................................................................9
3.4.1 Principles of checking endothelium function...............................................................................................................................9
3.5 In vitro experiment 1: Noradrenaline contractile response............................................................................................................9
3.5.1 Background ....................................................................................................................................................................................9
3.6 In vitro experiment 2: Acetylcholine relaxation curve .................................................................................................................. 10
3.6.1 Background ................................................................................................................................................................................. 10
3.5.2 Protocol........................................................................................................................................................................................ 10
3.6.2 Protocol........................................................................................................................................................................................ 11
Chapter 4 - CleAning and maintenance ............................................................................................................................................................................. 12
4.1 Cleaning the Tissue Bath ............................................................................................................................................................... 12
4.2 Maintenance of the force transducer ........................................................................................................................................... 13
3.3.1 Checking the force transducer................................................................................................................................................... 13
4.3.1 Force transducer replacement................................................................................................................................................... 14
4.4 Maintenance of the linear slides................................................................................................................................................... 15
Appendix 1 - Buer recipes ..................................................................................................................................................................................................... 16
Appendix 2 - Normalization theory ...................................................................................................................................................................................... 18
Appendix 3 - Reading a millimetre micrometer............................................................................................................................................................... 20
Notes................................................................................................................................................................................................................................................ 21

4CHAPTER 1
CHAPTER 1  TISSUE BATH OVERVIEW
Micrometer
Pin support connected to micrometer
Force transducer pin
Pin support connected to
force transducer
Figure 1.1 Tissue Bath with close-up of chamber
Transducer house

5TISSUE BATH MYOGRAPH SYSTEM - 720MO - USER GUIDE
CHAPTER 2  SETTING UP THE TISSUE BATH
2.1 Changing and adjusting the mounting supports
Each chamber can accommodate mounting supports for larger segments (>500 µm). Because the mounting supports can be
changed easily, experiments can be performed with different vessels of varying internal diameter from 500 µm up to 10 mm.
Continuous use and repeated greasing of the transducer arm holes will cause some misalignment of the mounting supports. The
mounting supports will need occasional adjustments. Changing and adjustment of the mounting supports is performed using
the following step-by-step procedure.
OBS
THE TRANSDUCERS ARE FRAGILE AND SENSITIVE TO MECHANICAL STRAIN. BE VERY CAREFUL WHEN CHANGING OR AD
JUSTING THE MOUNTING SUPPORTS!
2.1.1 Changing the mounting supports (gure 2.1):
1. Use the micrometer to separate the supports as far apart as possible.
2. Use the small screwdriver provided to gently loosen screw “D” on the support attached on the transducer side. Screw “D” is
the screw on the support closest to the transducer.
3. Gently pull the support away from the transducer pin.
4. Loosen screw “B” on the micrometer side with the appropriate Allen key.
5. Pull the pin support away.
NOTE
NUMBER THE MOUNTING SUPPORTS WITH THE NUMBER OF THE CHAMBER THEY WERE REMOVED FROM USING A
PERMANENT MARKER. STORE THE MOUNTING SUPPORTS IN THE PROVIDED PLASTIC CASE. NUMBERING THE MOUNTING
SUPPORTS WILL SAVE TIME WHEN THE MOUNTING SUPPORTS ARE CHANGED AGAIN, MINIMIZING THE AMOUNT OF
ADJUSTMENTS NEEDED AFTER EACH CHANGE.
A B C D
Figure 2.1 Illustration of the screws for changing supports and adjustment of the pins
Micrometer