DMT 820MO User manual

ORGAN BATH SYSTEM –820MO
USER MANUAL, VOL. 1.2

TRADEMARKS
PowerLab® and LabChart® are registered trademarks of ADInstruments Pty Ltd.
The names of specific recording units, such as PowerLab 4/35, are trademarks of ADInstruments Pty Ltd.
Pentium is a registered trademark of the Intel Corporation. Windows 7 and Windows 10 are registered trademarks
of Microsoft Corporation.
All other trademarks are the properties of their respective owners.
DMT reserves the right to alter specifications as required.
As far as possible, this document was accurate at the time of printing.
Since then, changes may have been made to the software and hardware it
describes. New information may be supplied separately.
This documentation is provided with the DMT Organ Bath System –Model 820MO
Document Number: 820MO –UG2.2A
All rights reserved. Nopart ofthis manual may bereproducedortransmitted inanyform orbyanymeans without
the written permission of Danish Myo Technology A/S.
Every attempt is made to ensure an accurate information, misprints, construction- and specification changes can occur.
Danish Myo Technology A/S reserves the right to alter/change content as required and without any notice.
Copyright © Danish Myo Technology A/S

CONTENTS
TRADEMARKS 3
SAFETY 4
UNPACKING THEMYOGRAPH SYSTEM 5
EC DECLARATION OF CONFORMITY 6
CHAPTER 1 -SYSTEM OVERVIEW 7
1.1 Interface 7
1.2 Interface Rear Panel 8
1.3 Organ Bath Chamber - 820MO 9
CHAPTER 2 -SETTING UP 10
2.1 Setting up the Organ Bath System - 820MO 10
2.2 Setting up step-by-step 11
CHAPTER 3 - THEINTERFACE MENUS 13
3.1 Interface Menus - Navigation 13
3.2 Interface Menus – Calibrated Mode 16
CHAPTER 4 - THE ORGAN BATH CHAMBER UNIT 28
4.1 Calibration of the Force Transducer 28
4.2 Checking the Force Transducer 30
4.3 Force Transducer Replacement 31
4.4 Organ Bath Maintenance 33
4.5 Cleaning the System 34
CHAPTER 5 - MOUNTING SUPPORTS AND MUSCLE MOUNTING 36
APPENDIX 1 - SYSTEM SPECIFICATIONS 40

SAFETY
The Organ Bath System - 820MO has been designed for teaching and research only. It is not intended for clinical or
critical life-care use and should never be used for these purposes. The 820MO also should not be used for the
prevention, diagnosis, curing, treatment, or alleviation of disease, injury, or handicap.
•DO NOT OPEN THE UNIT; THE INTERNAL ELECTRONICS POSE A RISK OF ELECTRIC SHOCK.
•DO NOT USE THIS APPARATUS NEAR WATER.
•TO REDUCE THE RISK OF FIRE OR ELECTRIC SHOCK, DO NOT EXPOSE THIS APPARATUS
TO RAIN OR MOISTURE.
•OBJECTS FILLED WITH LIQUIDS SHOULD NOT BE PLACED ON THE APPARATUS.
•DO NOT BLOCK ANY VENTILATION OPENINGS. INSTALL IN ACCORDANCE WITH THE
MANUFACTURER’S INSTRUCTIONS.
•DO NOT INSTALL NEAR ANY HEAT SOURCES SUCH AS RADIATORS, HEAT REGISTERS,
STOVES, OR OTHER EQUIPMENT OR DEVICES THAT PRODUCE HEAT.
•ONLY USE ATTACHMENTS AND ACCESSORIES SPECIFIED BY THE MANUFACTURER.
•UNPLUG THIS APPARATUS DURING LIGHTNING STORMS OR WHEN UNUSED FOR LONG
PERIODS OF TIME.
•BE ADVISED THAT DIFFERENT OPERATING VOLTAGES REQUIRE THE USE OF DIFFERENT
TYPES OF LINE CORD AND ATTACHMENT PLUGS. CHECK THE VOLTAGE IN YOUR AREA
AND USE THE CORRECT TYPE. SEE THE TABLE BELOW:
Voltage
Line plug according to standard
110 - 125V
UL81 and CSA C22.2 No. 42
220 - 230V
CEE 7 page VII, SR section 107-2-D1/IEC 83, page C4
240V
BS 1363 of 1984. Specification for 13A fused plugs and
switched and unswitched socket outlets.
Protect the power cord from being walked on or pinched: particularly at power plugs and the point where they
connect to the apparatus.
Refer all servicing to qualified service personnel. Servicing is required when the apparatus has been damaged in any
way; such as, the power-supply cord or plug is damaged, liquid has spilled onto or objects have fallen into the
apparatus, the apparatus has been exposed to rain or moisture, does not operate normally, or has been dropped. 4
CAUTION

UNPACKING THE ORGAN BATH SYSTEM
Take a few minutes to carefully inspect your new Organ Bath System - 820MO for damage that may have occurred
during handling and shipping. If you suspect any damage, please contact DMT immediately, and the matter will be
pursued as soon as possible. If the packing material appears damaged, please retain it until a potential claim has
beensettled.
We recommend that you store the packing material for any possible future transport of the Organ Bath System. In
case of transportation and the original packing material is unavailable, please get in touch with DMT for advice and
packinginstructions.
5

7
EC DECLARATION OF CONFORMITY
DMT A/S
Certify and declare that the following apparatus:
ORGAN BATH SYSTEM - MODEL 820MO
Restrictive use: Only for laboratory use.
Manufactured by:
DMT A/S
Rho 14
8382 Hinnerup
Denmark
Conforms with the essential requirements of the EMC Directive 2004/108/EC.
Based on the following specifications applied by:
EN 61326-1:2006
EN 61326-2-6:2006
EN 61326-2-6/Corr.:2007
And with the LVD Directive 2006/95/EC.
Based on the following specifications applied by:
EN 61010-1:2010
EN 61010-2-030:2010
General warnings regarding EMC:
Do not use this device in close proximity to sources of strong electromagnetic radiation
(e.g. unshielded intentional RF sources), as these may interfere with the proper operation.

CHAPTER 1 - SYSTEM OVERVIEW
1.1 INTERFACE
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Myo-Interface display
Heat indicator
Power indicator
Valve buttons
Figure 1.1 Interface front panel

1.2 INTERFACE REAR PANEL
8
Temperature probe
connection
pH meter connection
Transducer connections
Gas regulator
(needle valve)
Gas input
Vacuum input
ON/OFF switch
4 Recorder
outputs (analogue)
USB output
(digital)
Grounding connected to
Data Acquisition
Power connector
Figure 1.2 Interface Rear Panel

1.3 ORGAN BATH CHAMBER - 820MO
9
Figure 1.4 Close up of the 820MO myograph chamber unit
Figure 1.3 820MO Organ bath chamber unit and 820MO-XL Chamber Unit
Connection to Organ Bath Interface
Micrometer positioner
Organ bath clamp support
connected to micrometer
Force transducer pin
Organ bath clamp support
connected to force transducer

CHAPTER 2 - SETTING UP
2.1 SETTING UPTHE MUSCLE STRIP MYOGRAPH SYSTEM - 820MS
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Figure 2.1 The complete Organ Bath System –820MO

2.2 SETTING UPSTEP-BY-STEP
This chapter contains a complete step-by-step description of setting up a complete organ bath 820MO system, as
illustrated in Figure 2.1.
1. Interface –PC Connection:
Data acquisition is possible either by connecting the 820MO Interface directly to a PC with LabChart Pro and
the DMT Device enabler installed or through the BNC connectors, and BNC cables from the 820MO Interface
e.g., to the Powerlab box and the Powerlab box is then connected using a USB cable to the computer with
LabChart installed(optional).
I. Direct PC Connection: Connect the 820MO Interface using the applied USB cable directly to the PC
with LabChart Pro (version 8 or newer) and the DMT Device enabler installed.
II. PowerLab (Optional): Connect the 820MO Interface to the PowerLab unit using BNC cables. Connect Rec 1on
the Interface to Input 1on the Power Lab, Rec 2 to Input 2, etc. Connect the PowerLab unit to one of the USB-
ports on the PC using the USB cable delivered with the PowerLab system.
2. Oxygen Supply:
Connect the gas supply (95% O2, 5% CO2 or 21% O2, 5% CO2, balance N2) with tubing running from the gas
supply to the gas inlet MARKED “OXYGEN” on the back of the Interface. This stainless steel inlet pipe will
supply oxygen to the chambers. The oxygen tubing needs to be inserted into the chamber in order to aerate
the heated buffer. Needle valves on the back of the Interface can be adjusted to regulate the amount of
bubbling that occurs. Turning the regulator clockwise increases the bubbling, while turning it counter-clockwise
decreases the bubbling (see Figure 2.2). Each regulator has a locking device attached that can be used when
the desired bubbling is achieved.
3. Vacuum Connection:
The system has a built-in manifold with separate valves that allow each chamber to be drained individually.
After connecting the vacuum source at the back of the Interface, the vacuum pipes need to be inserted into the
chambers for this feature to work properly. The pipes are inserted into the chamber by gently pulling
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NOTE: THE NEEDLE VALVES NEED TO BE GREASED (USING THE GREASE FOR THE LINEAR SLIDES
–THE BROWN GREASE) AND TURNED AT REGULAR INTERVALS TO PREVENT THEM FROM
STICKING OR PERMANENTLY LOCKING.

up on the curved part of the pipe, turning it 90° counter-clockwise and gently lowering it into the chamber, see
Figure 2.2. A chamber can then be emptied by pressing the corresponding numbered button. Pressing the “all”
button will drain all the chambers at the same time.
4. Chamber Covers : The chamber covers will help maintain the temperature and other buffer conditions (gas
tension, pH) fairly constant. Holes in the chamber covers serve different purposes, and they are illustrated in
Figure 2.3A-B. The slots allow the covers to be placed over the chamber around the support arms and gas/
vacuum tubes.
Figure 2.3A - Chamber cover Figure 2.4B - Chamber cover with built-in electrodes (optional item)
5. Initial Force Transducer Status: Prior to the shipment of the Organ Bath 820MO System, it has gone
through two days of continuous testing, including a final force transducer calibration. However, DMT
recommends that a new force transducer calibration is performed before using the organ bath system for the
first time. The force transducer calibration procedure is described indetail in the FORCE CALIBRATION sub-
menu under SETTINGS, asexplained in Chapter 3.
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NOTE: WHEN DRAINING THE CHAMBERS USING THE AUTOMATIC VACUUM FUNCTION,
PRESS THE APPROPRIATE BUTTON FOR AN ADDITIONAL 3-5 SECONDS AFTER THE INITIAL
EMPTYING. THIS WILL HELP DRAIN RESIDUAL BUFFER AND SOLUTIONS RETAINED IN THE
TUBING AND VALVES.
Figure 2.2 Oxygen supply and suction connection
Gas
Suction

CHAPTER 3 - THE INTERFACE MENUS
Chapter 3 is a complete manual for the 820MO Interface menus. This chapter contains a detailed description of
how to navigate the touch-screen menus and how to use the special features of the 820MO myograph.
3.1 INTERFACE MENUS -NAVIGATION
Menusonthe820MOInterfaceareall accessibleby atouchscreen. To
access a menu, simply touch the screen. A setting can be changed by
touchingthe“SELECT”icononthescreencorrespondingto the
“SELECT” icon on the touch screen corresponding to the desired
channel to be changed.
The line to be modified will turn blue, indicating that the Interface is
waiting for input. When “ALL” is chosen, all lines corresponding to all
4 channels will turn blue. Changing the numeric value for the chosen
parameter can be done by touching the up or down arrow keys.
Touch “ENTER” when the desired setting has been chosen. Touching
the “X” will exit the menu and automatically take the user to the
“ACTUAL FORCE” Display.
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SELECT

3.2 INTERFACE MENUS
POWER-UP SCREEN
After turning on the 820MO Interface, an “Introduction” screen
appears. The system is auto-calibrating the A/D converters while this
screen is displayed.
After a few seconds, the “ACTUAL FORCE” display will appear.
At any given time, if the force applied on any channel is out of range,
the force reading for the overloaded channel will turn yellow as a
warning.
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Three menus are accessible from the default “Actual Force” screen or
display. These menus are Zero, Heat, and Settings.
ZERO MENU
This menu is used to zero the output of the transducers. When using
a data acquisition program like LabChart by AD Instruments ®,
using this feature will reset the baseline of the chart traces without
affecting the calibrations or physically changing any pre-load
tensions placed on the mounted vessels.Thechannelscanbe
changedindividuallybypressing “SELECT” orallatoncebypressing
“ALL.” Pressing“ENTER” will execute the zero function and return the
user to the ACTUAL FORCE display.
HEAT MENU
The heating unit and temperature are controlled from this menu. To
turn the heat on or change the preset temperature for the system,
access the temperature control menu. Pressing the “HEAT” key will
enter the menu and allow the user to change the default system
temperature, as well as turn the heat on or off. Pressing “DEFAULT”
will automatically reset the temperature setpoint to 37°C. Manually
change the temperature by pressing the up or downarrows.
To turn the heat on, touch “ON” and the “ON” icon will turn green,
indicatingtheheathasbeenturnedon.Thesystemwill heat to the
designated temperature setpoint.
Pressing the white “X” in the red box will send the user back to the
“ACTUAL FORCE” display.
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SETTINGS MENU
The “Settings Menu” contains several sub-menus that can be
accessed to change functional aspects of the Interface. These sub-
menus include:
I. Force Calibration
II. pH Calibration
III. Force REC. OUTPUT
IV. Measurement Range
V. Interface Settings
1. Force Calibration
Entering the FORCE CALIBRATION sub-menu begins the
transducer calibration procedure. Begin the calibration procedure
by pressing “FORCE CALIBRATION” to enter the sub-menu. The
sub-menu will list all 4 chambers for calibration.
(For more information and tips, please refer to Section 4.1-
Calibration of the Force Transducer)
Tobeginthecalibration,press“SELECT” forthechamberwhich
calibration will be performed on. The text for the chamber to be
calibrated will turn blue. Pressing “ENTER” will enter the 6-step
procedure for calibrating the force transducer on the desired
chamber.
The calibration procedure is listed in 6 individual steps and needs to
be performed for each channel or transducer when calibrating the
system.
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Step 1 involves setting up the chamber for calibration. Make sure
the chamber contains the pins or clamps, depending on the type of
tissue being studied. Fill the chamber with double-distilled water for
thevolumetobeusedexperimentally.Press“NEXT STEP”.
Step 2 involves setting up the calibration kit appropriately for the
actual weight calibration. Verify that the transducer arm pin does
not touch the mountingpinforlargervessels or clamp for muscle,
asinstructed. The calibrationpinshould beascloseaspossibleto
themountingpin or clamp withouttouchingtogetthemost
accuratecalibration. Press“NEXT STEP” whenthecalibrationkit
hasbeenproperly placed.
Step 3 initiates the heating process for the chambers. For the
calibration to be accurate, the transducers must beheated tothe
experimental temperature tobe used to accommodate heat-
induced expansion of the electronic parts in thetransducer.
Otherwise, inaccurate readings and transducer drift may occur,
introducing large errors into the experiment.
.
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CHAMBER 1 CALIBRATION
Step no.:
Turn the heat on. Wait until
the temperature is stable.
NEXT STEPBACK
31 2 4 6
5
Temperature setpoint: 37.0 °C
Probe temperature: 36.8 °C
HEAT ON HEAT OFF

Step 4 is the first step in the actual weight calibration process. A
4-digit number will bedisplayed inblue at the bottom of the screen.
If nothing has been perturbed during the heating process, the zero,
0 gram, or 0.00mN calibration should be stable as indicated by the
4-digitnumber,and“NEXT STEP” canbepressedafter30-40
seconds. If the 4-digit number is not stable, then wait until the
number has stopped fluctuating before pressing “NEXT STEP”.
Step 5 is the 2gram weight calibration. Place the 2gram weight in
the pan closest to the transducer to simulate a mounted muscle
pulling on the clamp attached to the transducer. Remember, a 2 gram
weight in a 90° vector is cut in half, and the transducer will only detect
1 gram or 9.81mN of force. The weight placement should cause a
positive increase in the 4-digit number. Wait at least 30 to 40 seconds
for the applied force to stabilize before pressing “NEXT STEP”. Once
the 4-digit number has stabilized, press “NEXT STEP”.
Step 6 is to verify that the calibration was performed correctly. The
“Force Chamber 1” reading should be 9.81 ± 0.1mN. If the “Force
Chamber 1” reading is off by more than 0.1mN, then remove the
weight, press “BACK” to return to Step 4, and repeat the calibration
process. If the “Force Chamber 1” reading is satisfactory, then press
“NEXT STEP”. Calibrate the other chambers in the same manner.
2.
pH Calibration Menu (Optional)
The pH Module in the 820MO is locked by a code. If the lockscreen
appears, a code is needed from DMT to unlock the pH module.
By ordering the pH sensor from DMT, the pH sensor is delivered
with the unlocking code and a quick guide on how to do it. Entering
the code will open the pH Calibrationmenu.
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The 820MO system has a build-in pH meter and a pH-meter
electrode plug-in port marked PH on the backside of the 820M
interface.
The pH electrode can be ordered at DMT by contacting your sales
representative or emailing [e-mail].
ThepHcalibrationprocedureislistedin4individualstepsandneeds
to be performed one at a time.
Step 1-Step 1involves cleaning thepH electrode andthetemperature
probe with double distilled water. When ready, Press NEXT STEP.
Step 2 - Place the pH electrode and temperature probe inthe high
buffer solution (here pH 7) and turn on stirring of the high buffer
solution.WhentherelativepHoutputinthebluelineisstable,goto
NEXT STEP.
Step 3 - Place the pH electrode and temperature probe in the low
buffer solution (here pH 4) and turn on stirring of the low buffer
solution. When the relative pH output in the blue line is stable, go
to NEXTSTEP.
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NOTE: BEFORE THE PH CALIBRATION IS PERFORMED
BE SURE TO SELECT THE WAY THE PH ELECTRODE IS
TO BE USED. SEE THE SUB- MENU PH SET-UP UNDER
INTERFACE SETTINGS.
LOGIN CODE TO pH
Call DMT A/S to get access code.
System ID number. 1056
CLR ENTER
0
12345
6 7 8 9
Step no.:
Follow the pH calibration
procedure in the User Manual.
NEXT STEP
1 2 3 4
Clean the pH electrode and the
pH CALIBRATION
temperature probe.
When ready go to next step.

Step4-Thecalibrationisnowfinished.Thevaluesinthetwobottom
lines are the actual pH and temperature reading.
3.
Force Rec. output (analog output only)
The FORCE RECORDING OUTPUT, or FORCE REC. OUT,sub-
menu determines the upper limit for force sent through the BNC
output connectors to e.g. Powerlab or a similar data acquisition
system. The factory default setting for FORCE REC. OUT is 20mN,
meaning that if the force of the mounted vessel exceeds 20mN, the
force recorded in the data acquisition software will not record more
than 20mN and will appear as a flat-line trace at 20mN even though
the force readings on the Interface may exceed 20mN. Therefore,
change the FORCE REC. OUT settings to an appropriate setting to
capture any maximal response from the mounted tissue of interest.
This value should not exceed the settings for the transducer range,
which is defined by the sub-menu, MEASUREMENT RANGE, and
is explained in the next section. As can be seen on the below
screen figure, the Force Rec Output can be set to different values
on different chambers.
The “SELECT” for a single chamber is used if different values are
needed for the four chambers andchanged by pressing the Up and
Down arrow keys and stores in the interface setting by pressing
ENTER.
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